DE1901517C2 - Reagens und Verfahren zur Bestimmung von α-Amylasen oder Dextranasen - Google Patents
Reagens und Verfahren zur Bestimmung von α-Amylasen oder DextranasenInfo
- Publication number
- DE1901517C2 DE1901517C2 DE1901517A DE1901517A DE1901517C2 DE 1901517 C2 DE1901517 C2 DE 1901517C2 DE 1901517 A DE1901517 A DE 1901517A DE 1901517 A DE1901517 A DE 1901517A DE 1901517 C2 DE1901517 C2 DE 1901517C2
- Authority
- DE
- Germany
- Prior art keywords
- reagent
- groups
- atoms
- reagent according
- dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 55
- 238000000034 method Methods 0.000 title claims description 26
- 108010001682 Dextranase Proteins 0.000 title claims description 7
- 102000004139 alpha-Amylases Human genes 0.000 title description 2
- 108090000637 alpha-Amylases Proteins 0.000 title description 2
- 150000004676 glycans Chemical class 0.000 claims description 23
- 229920001282 polysaccharide Polymers 0.000 claims description 22
- 239000005017 polysaccharide Substances 0.000 claims description 22
- 108090000790 Enzymes Proteins 0.000 claims description 21
- 102000004190 Enzymes Human genes 0.000 claims description 21
- 229940088598 enzyme Drugs 0.000 claims description 21
- 125000004429 atom Chemical group 0.000 claims description 17
- 239000000126 substance Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 10
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 8
- 229920002472 Starch Polymers 0.000 claims description 7
- 239000012634 fragment Substances 0.000 claims description 7
- 235000019698 starch Nutrition 0.000 claims description 7
- 229920002307 Dextran Polymers 0.000 claims description 6
- 125000004432 carbon atom Chemical group C* 0.000 claims description 6
- 108010065511 Amylases Proteins 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 5
- 238000004132 cross linking Methods 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 4
- 229940025131 amylases Drugs 0.000 claims description 4
- 125000004430 oxygen atom Chemical group O* 0.000 claims description 3
- 229920000945 Amylopectin Polymers 0.000 claims description 2
- 229920000856 Amylose Polymers 0.000 claims description 2
- 229920001353 Dextrin Polymers 0.000 claims description 2
- 239000004375 Dextrin Substances 0.000 claims description 2
- 125000002947 alkylene group Chemical group 0.000 claims description 2
- 235000019425 dextrin Nutrition 0.000 claims description 2
- 125000001931 aliphatic group Chemical group 0.000 claims 1
- 239000004382 Amylase Substances 0.000 description 14
- 238000012360 testing method Methods 0.000 description 11
- 239000002245 particle Substances 0.000 description 9
- 230000002285 radioactive effect Effects 0.000 description 9
- 230000001588 bifunctional effect Effects 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000035484 reaction time Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- SHKUUQIDMUMQQK-UHFFFAOYSA-N 2-[4-(oxiran-2-ylmethoxy)butoxymethyl]oxirane Chemical compound C1OC1COCCCCOCC1CO1 SHKUUQIDMUMQQK-UHFFFAOYSA-N 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- YKCWQPZFAFZLBI-UHFFFAOYSA-N cibacron blue Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC=C1S(O)(=O)=O YKCWQPZFAFZLBI-UHFFFAOYSA-N 0.000 description 4
- 239000011541 reaction mixture Substances 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 2
- JQYMGXZJTCOARG-UHFFFAOYSA-N Reactive blue 2 Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC(S(O)(=O)=O)=C1 JQYMGXZJTCOARG-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- GYZLOYUZLJXAJU-UHFFFAOYSA-N diglycidyl ether Chemical compound C1OC1COCC1CO1 GYZLOYUZLJXAJU-UHFFFAOYSA-N 0.000 description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 150000003944 halohydrins Chemical class 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- DEWLEGDTCGBNGU-UHFFFAOYSA-N 1,3-dichloropropan-2-ol Chemical compound ClCC(O)CCl DEWLEGDTCGBNGU-UHFFFAOYSA-N 0.000 description 1
- IVIDDMGBRCPGLJ-UHFFFAOYSA-N 2,3-bis(oxiran-2-ylmethoxy)propan-1-ol Chemical compound C1OC1COC(CO)COCC1CO1 IVIDDMGBRCPGLJ-UHFFFAOYSA-N 0.000 description 1
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- -1 B. dextran Chemical class 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- ZFIVKAOQEXOYFY-UHFFFAOYSA-N Diepoxybutane Chemical compound C1OC1C1OC1 ZFIVKAOQEXOYFY-UHFFFAOYSA-N 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- 239000005057 Hexamethylene diisocyanate Substances 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- BHELZAPQIKSEDF-UHFFFAOYSA-N allyl bromide Chemical compound BrCC=C BHELZAPQIKSEDF-UHFFFAOYSA-N 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 235000021170 buffet Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 125000005442 diisocyanate group Chemical group 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- GKIPXFAANLTWBM-UHFFFAOYSA-N epibromohydrin Chemical compound BrCC1CO1 GKIPXFAANLTWBM-UHFFFAOYSA-N 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical class O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- RRAMGCGOFNQTLD-UHFFFAOYSA-N hexamethylene diisocyanate Chemical compound O=C=NCCCCCCN=C=O RRAMGCGOFNQTLD-UHFFFAOYSA-N 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000006855 networking Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010557 suspension polymerization reaction Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B31/00—Preparation of derivatives of starch
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B31/00—Preparation of derivatives of starch
- C08B31/003—Crosslinking of starch
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE00465/68A SE336915B (en:Method) | 1968-01-15 | 1968-01-15 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE1901517A1 DE1901517A1 (de) | 1970-07-16 |
| DE1901517C2 true DE1901517C2 (de) | 1983-01-20 |
Family
ID=20256615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE1901517A Expired DE1901517C2 (de) | 1968-01-15 | 1969-01-14 | Reagens und Verfahren zur Bestimmung von α-Amylasen oder Dextranasen |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US3676303A (en:Method) |
| JP (2) | JPS5134317B1 (en:Method) |
| BE (1) | BE726843A (en:Method) |
| CH (1) | CH499110A (en:Method) |
| DE (1) | DE1901517C2 (en:Method) |
| DK (1) | DK121822B (en:Method) |
| FI (1) | FI49883C (en:Method) |
| FR (1) | FR2000245A1 (en:Method) |
| GB (1) | GB1251433A (en:Method) |
| NL (1) | NL165218C (en:Method) |
| SE (1) | SE336915B (en:Method) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1291077B (de) * | 1966-06-08 | 1969-03-20 | Demag Zug Gmbh | Vorrichtung zur Verriegelung zweier Haengekatzfahrbahnen miteinander |
| GB1320086A (en) * | 1970-09-11 | 1973-06-13 | Boehringer Mannheim Gmbh | Agent and method for the determination of hydrolysing enzymes |
| CA1104912A (en) * | 1976-07-13 | 1981-07-14 | Robert C. Menson | Method and test kit for serum amylase assay |
| CA1095819A (en) * | 1977-01-14 | 1981-02-17 | Eastman Kodak Company | Element for analysis of liquids |
| DE2819298A1 (de) * | 1978-05-02 | 1979-11-08 | Boehringer Mannheim Gmbh | Alpha -amylasesubstrat und verfahren zu seiner herstellung |
| DE3001878A1 (de) * | 1980-01-19 | 1981-07-23 | Behringwerke Ag, 3550 Marburg | Polysaccharid-derivate, verfahren zu ihrer herstellung, ihre verwendung sowie diese enthaltende mittel |
| US4403032A (en) * | 1980-04-11 | 1983-09-06 | Wisconsin Alumni Research Foundation | Continuous spectrophotometric assay of microbial cellulase |
| US4321364A (en) * | 1980-04-17 | 1982-03-23 | Minister For Public Works For The State Of New South Wales | Preparation of soluble chromogenic substrates |
| EP0040728A1 (en) * | 1980-05-19 | 1981-12-02 | Pharmacia Diagnostics Ab | An improvement in and relating to assaying methods involving biospecific affinity reactions |
| US4859581A (en) * | 1986-03-10 | 1989-08-22 | Board Of Regents, The University Of Texas System | Endoglycosidase assay |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3089828A (en) * | 1960-12-27 | 1963-05-14 | Schwarz Biores Inc | Evaluation of proteolytic enzyme activity |
| FR1508496A (fr) * | 1966-01-20 | 1968-01-05 | Warner Lambert Pharmaceutical | Produit et procédé pour le dosage de l'amylase |
-
1968
- 1968-01-15 SE SE00465/68A patent/SE336915B/xx unknown
-
1969
- 1969-01-14 FR FR6900458A patent/FR2000245A1/fr not_active Withdrawn
- 1969-01-14 FI FI690105A patent/FI49883C/fi active
- 1969-01-14 BE BE726843A patent/BE726843A/fr unknown
- 1969-01-14 DE DE1901517A patent/DE1901517C2/de not_active Expired
- 1969-01-14 US US791177A patent/US3676303A/en not_active Expired - Lifetime
- 1969-01-14 DK DK18869AA patent/DK121822B/da not_active IP Right Cessation
- 1969-01-14 GB GB1251433D patent/GB1251433A/en not_active Expired
- 1969-01-14 CH CH42269A patent/CH499110A/fr not_active IP Right Cessation
- 1969-01-15 NL NL6900644.A patent/NL165218C/xx not_active IP Right Cessation
- 1969-01-16 JP JP44003134A patent/JPS5134317B1/ja active Pending
-
1976
- 1976-02-26 JP JP2053676A patent/JPS5498700A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE1901517A1 (de) | 1970-07-16 |
| JPS5498700A (en) | 1979-08-03 |
| SE336915B (en:Method) | 1971-07-19 |
| BE726843A (fr) | 1969-06-16 |
| US3676303A (en) | 1972-07-11 |
| GB1251433A (en:Method) | 1971-10-27 |
| JPS5134317B1 (en:Method) | 1976-09-25 |
| NL6900644A (en:Method) | 1969-07-17 |
| NL165218C (nl) | 1981-03-16 |
| FI49883B (en:Method) | 1975-06-30 |
| FI49883C (fi) | 1975-10-10 |
| FR2000245A1 (en:Method) | 1969-09-05 |
| NL165218B (nl) | 1980-10-15 |
| DK121822B (da) | 1971-12-06 |
| CH499110A (fr) | 1970-11-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |