GB1251433A - - Google Patents
Info
- Publication number
- GB1251433A GB1251433A GB1251433DA GB1251433A GB 1251433 A GB1251433 A GB 1251433A GB 1251433D A GB1251433D A GB 1251433DA GB 1251433 A GB1251433 A GB 1251433A
- Authority
- GB
- United Kingdom
- Prior art keywords
- reagent
- groups
- atoms
- indicatable
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003153 chemical reaction reagent Substances 0.000 abstract 8
- 108090000790 Enzymes Proteins 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 4
- 229940088598 enzyme Drugs 0.000 abstract 4
- 239000012634 fragment Substances 0.000 abstract 2
- 150000004676 glycans Chemical class 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 229920001282 polysaccharide Polymers 0.000 abstract 2
- 239000005017 polysaccharide Substances 0.000 abstract 2
- 230000002285 radioactive effect Effects 0.000 abstract 2
- 108010001682 Dextranase Proteins 0.000 abstract 1
- 229920001131 Pulp (paper) Polymers 0.000 abstract 1
- 102000004139 alpha-Amylases Human genes 0.000 abstract 1
- 108090000637 alpha-Amylases Proteins 0.000 abstract 1
- 229940024171 alpha-amylase Drugs 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B31/00—Preparation of derivatives of starch
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B31/00—Preparation of derivatives of starch
- C08B31/003—Crosslinking of starch
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
1,251,433. Determination of endohydrolases. PHARMACIA A.B. 14 Jan., 1969 [15 Jan., 1968], No. 2109/69. Heading B1X. [Also in Division C3] A method for determining endohydrolases capable of hydrolysing polysaccharides, in aqueous samples, comprises contacting the sample with a water-insoluble, hydrophilic, swellable, enzymatically hydrolysable, three dimensional network of molecules of a polysaccharide or enzymatically hydrolysable derivatives thereof, the molecules being crosslinked by bridges with bonds of a covalent character, in which network there is also present indicatable groups or atoms (as defined) bound by means of bonds of a covalent character, a reaction taking place between the enzyme and reagent to release water-soluble fragments of the reagent and subsequent to the enzyme having reacted upon the reagent for a determined length of time, separating undissolved reagent from the liquid with the water-soluble fragments of the reagent dissolved therein, and then determining the indicatable groups or atoms in the liquid or undissolved reagent as a measure of enzyme activity. Specified enzymes are alpha-amylase and endodextranase. The indicatable groups or atoms may be colour-producing groups, fluorescent groups or radioactive isotope atoms or groups containing radioactive isotopes. The reagent can be mixed with paper pulp to form a reagent-containing paper.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE00465/68A SE336915B (en) | 1968-01-15 | 1968-01-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1251433A true GB1251433A (en) | 1971-10-27 |
Family
ID=20256615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1251433D Expired GB1251433A (en) | 1968-01-15 | 1969-01-14 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US3676303A (en) |
| JP (2) | JPS5134317B1 (en) |
| BE (1) | BE726843A (en) |
| CH (1) | CH499110A (en) |
| DE (1) | DE1901517C2 (en) |
| DK (1) | DK121822B (en) |
| FI (1) | FI49883C (en) |
| FR (1) | FR2000245A1 (en) |
| GB (1) | GB1251433A (en) |
| NL (1) | NL165218C (en) |
| SE (1) | SE336915B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1291077B (en) * | 1966-06-08 | 1969-03-20 | Demag Zug Gmbh | Device for locking two hanging trolley tracks together |
| GB1320086A (en) * | 1970-09-11 | 1973-06-13 | Boehringer Mannheim Gmbh | Agent and method for the determination of hydrolysing enzymes |
| NL179399C (en) * | 1976-07-13 | 1986-09-01 | Du Pont | METHOD FOR DETERMINING THE AMYLASE CONTENT OF A SAMPLE AND REAGENT TEST PACKAGE THEREFOR. |
| CA1095819A (en) * | 1977-01-14 | 1981-02-17 | Eastman Kodak Company | Element for analysis of liquids |
| DE2819298A1 (en) * | 1978-05-02 | 1979-11-08 | Boehringer Mannheim Gmbh | ALPHA AMYLASE SUBSTRATE AND PROCESS FOR ITS MANUFACTURING |
| DE3001878A1 (en) * | 1980-01-19 | 1981-07-23 | Behringwerke Ag, 3550 Marburg | POLYSACCHARIDE DERIVATIVES, METHOD FOR THE PRODUCTION THEREOF, THEIR USE AND THESE AGENTS |
| US4403032A (en) * | 1980-04-11 | 1983-09-06 | Wisconsin Alumni Research Foundation | Continuous spectrophotometric assay of microbial cellulase |
| US4321364A (en) * | 1980-04-17 | 1982-03-23 | Minister For Public Works For The State Of New South Wales | Preparation of soluble chromogenic substrates |
| EP0040728A1 (en) * | 1980-05-19 | 1981-12-02 | Pharmacia Diagnostics Ab | An improvement in and relating to assaying methods involving biospecific affinity reactions |
| US4859581A (en) * | 1986-03-10 | 1989-08-22 | Board Of Regents, The University Of Texas System | Endoglycosidase assay |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3089828A (en) * | 1960-12-27 | 1963-05-14 | Schwarz Biores Inc | Evaluation of proteolytic enzyme activity |
| FR1508496A (en) * | 1966-01-20 | 1968-01-05 | Warner Lambert Pharmaceutical | Product and method for the assay of amylase |
-
1968
- 1968-01-15 SE SE00465/68A patent/SE336915B/xx unknown
-
1969
- 1969-01-14 BE BE726843A patent/BE726843A/en unknown
- 1969-01-14 FI FI690105A patent/FI49883C/en active
- 1969-01-14 FR FR6900458A patent/FR2000245A1/fr not_active Withdrawn
- 1969-01-14 DE DE1901517A patent/DE1901517C2/en not_active Expired
- 1969-01-14 US US791177A patent/US3676303A/en not_active Expired - Lifetime
- 1969-01-14 GB GB1251433D patent/GB1251433A/en not_active Expired
- 1969-01-14 CH CH42269A patent/CH499110A/en not_active IP Right Cessation
- 1969-01-14 DK DK18869AA patent/DK121822B/en not_active IP Right Cessation
- 1969-01-15 NL NL6900644.A patent/NL165218C/en not_active IP Right Cessation
- 1969-01-16 JP JP44003134A patent/JPS5134317B1/ja active Pending
-
1976
- 1976-02-26 JP JP2053676A patent/JPS5498700A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| NL6900644A (en) | 1969-07-17 |
| DK121822B (en) | 1971-12-06 |
| CH499110A (en) | 1970-11-15 |
| FI49883B (en) | 1975-06-30 |
| FR2000245A1 (en) | 1969-09-05 |
| SE336915B (en) | 1971-07-19 |
| DE1901517A1 (en) | 1970-07-16 |
| DE1901517C2 (en) | 1983-01-20 |
| BE726843A (en) | 1969-06-16 |
| NL165218C (en) | 1981-03-16 |
| US3676303A (en) | 1972-07-11 |
| JPS5134317B1 (en) | 1976-09-25 |
| FI49883C (en) | 1975-10-10 |
| NL165218B (en) | 1980-10-15 |
| JPS5498700A (en) | 1979-08-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PCNP | Patent ceased through non-payment of renewal fee |