GB1151608A - A Method for Determining Vitamin B12 - Google Patents

A Method for Determining Vitamin B12

Info

Publication number
GB1151608A
GB1151608A GB47604/67A GB4760467A GB1151608A GB 1151608 A GB1151608 A GB 1151608A GB 47604/67 A GB47604/67 A GB 47604/67A GB 4760467 A GB4760467 A GB 4760467A GB 1151608 A GB1151608 A GB 1151608A
Authority
GB
United Kingdom
Prior art keywords
vitamin
particles
sample
radioisotope
oct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB47604/67A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Health AB
Original Assignee
Pharmacia AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia AB filed Critical Pharmacia AB
Publication of GB1151608A publication Critical patent/GB1151608A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay

Abstract

1,151,608. Estimation of vitamin B 12 . PHARMACIA AB. 19 Oct., 1967 [21 Oct., 1966], No. 47604/67. Heading B1X. [Also in Division C3] Vitamin B 12 in an aqueous sample is estimated by contacting particles of a water insoluble epichlorhydrin crosslinked dextran derivative which has covalently bound to it the substance "intrinsic factor" (a muco protein from the ventriculus mucous membrane, and which is capable of binding Vitamin B 12 ; see Division C3), with the sample and with a predetermined amount of vitamin B 12 labelled with a radioisotope, and subsequently separating the particles from the sample liquid and measuring the radioactivity o,f the particles and/or of the liquid. Suitably the radioisotope is <SP>58</SP>Co. In the example a 0.05M tris-buffer at pH 7.4 containing NaCl, bovine serum albumin, and sodium azide is used as aqueous medium. The dextran reagent may be supplied in ampoules together with ampoules containing the labelled vitamin B 12 , to make a test pack.
GB47604/67A 1966-10-21 1967-10-19 A Method for Determining Vitamin B12 Expired GB1151608A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE14397/66A SE326845B (en) 1966-10-21 1966-10-21

Publications (1)

Publication Number Publication Date
GB1151608A true GB1151608A (en) 1969-05-14

Family

ID=20299036

Family Applications (1)

Application Number Title Priority Date Filing Date
GB47604/67A Expired GB1151608A (en) 1966-10-21 1967-10-19 A Method for Determining Vitamin B12

Country Status (12)

Country Link
US (1) US3505019A (en)
AT (1) AT286243B (en)
BE (1) BE705412A (en)
CH (1) CH476493A (en)
DE (1) DE1673015B1 (en)
DK (1) DK120509B (en)
ES (1) ES346273A1 (en)
FI (1) FI48509C (en)
GB (1) GB1151608A (en)
NL (1) NL159191B (en)
NO (1) NO122457B (en)
SE (1) SE326845B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3853987A (en) * 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
GB1458978A (en) * 1973-12-11 1976-12-22 Radiochemical Centre Ltd Saturation analysis
US3936440A (en) * 1974-05-22 1976-02-03 Drexel University Method of labeling complex metal chelates with radioactive metal isotopes
US4225784A (en) * 1976-06-17 1980-09-30 Smith Kline Instruments, Inc. Covalently bound biological substances to plastic materials and use in radioassay
US5227311A (en) * 1988-10-11 1993-07-13 Abbott Laboratories Intrinsic factor to determine B12
DE3900650A1 (en) * 1989-01-11 1990-07-12 Boehringer Mannheim Gmbh VITAMIN B12 DETERMINATION
US6942977B1 (en) 1991-04-09 2005-09-13 Bio-Rad Laboratories, Inc. Immunoassays for determining vitamin b12, and reagents and kits therefor

Also Published As

Publication number Publication date
DK120509B (en) 1971-06-07
NL159191B (en) 1979-01-15
NL6714282A (en) 1968-04-22
FI48509B (en) 1974-07-01
FI48509C (en) 1974-10-10
US3505019A (en) 1970-04-07
BE705412A (en) 1968-03-01
AT286243B (en) 1970-11-25
NO122457B (en) 1971-06-28
CH476493A (en) 1969-08-15
SE326845B (en) 1970-08-03
ES346273A1 (en) 1969-01-01
DE1673015B1 (en) 1972-05-04

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee