GB1358827A - Process for detecting the presence of human carcinoembryonic antigen material in blood - Google Patents

Process for detecting the presence of human carcinoembryonic antigen material in blood

Info

Publication number
GB1358827A
GB1358827A GB4418573A GB4418573A GB1358827A GB 1358827 A GB1358827 A GB 1358827A GB 4418573 A GB4418573 A GB 4418573A GB 4418573 A GB4418573 A GB 4418573A GB 1358827 A GB1358827 A GB 1358827A
Authority
GB
United Kingdom
Prior art keywords
blood
carcinoembryonic antigen
cea
human carcinoembryonic
incubating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4418573A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of GB1358827A publication Critical patent/GB1358827A/en
Expired legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/02—Column or bed processes
    • B01J47/04—Mixed-bed processes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1045—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against animal or human tumor cells or tumor cell determinants
    • A61K51/1048—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against animal or human tumor cells or tumor cell determinants the tumor cell determinant being a carcino embryonic antigen
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/014—Ion-exchange processes in general; Apparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57473—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving carcinoembryonic antigen, i.e. CEA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2123/00—Preparations for testing in vivo
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y10S436/813—Cancer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/825—Pretreatment for removal of interfering factors from sample
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/828—Cancer

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hospice & Palliative Care (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Optics & Photonics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Food Science & Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1358827 Determination of human carcinoembryonic antigen material F HOFFMANN - LA ROCHE & CO A G 1 June 1971 [1 June 1970 27 Jan 1971 12 April 1971] 44185/73 Divided out of 1356911 Heading G1B Human carcinoembryonic antigen (CEA) material in blood is detected by a method comprising (a) adding a measured amount of antibody to the CEA material to a solution of a purified perchloric acid extract of blood serum or blood plasma in a buffered solvent; (b) incubating; (c) adding a measured amount of radioactively tagged CEA material; (d) incubating; (e) adding a precipitating agent, for example zirconyl phosphate gel, to precipitate antigen-antibody complexes; (f) measuring the radio-active content of the resulting precipitate. The initial serum or plasma sample may be treated with at most 1.2M perchloric acid and centrifuged, the supernatant then being purified by dialysis. In an example goat antiserum is used in step (a).
GB4418573A 1970-06-01 1971-06-01 Process for detecting the presence of human carcinoembryonic antigen material in blood Expired GB1358827A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4252670A 1970-06-01 1970-06-01
US11028871A 1971-01-27 1971-01-27
US13340471A 1971-04-12 1971-04-12

Publications (1)

Publication Number Publication Date
GB1358827A true GB1358827A (en) 1974-07-03

Family

ID=27366145

Family Applications (4)

Application Number Title Priority Date Filing Date
GB4418773A Expired GB1358828A (en) 1970-06-01 1971-06-01 Process for detecting the presence of human carcinoembryonic antigen material or component a or b thereof in blood
GB4418573A Expired GB1358827A (en) 1970-06-01 1971-06-01 Process for detecting the presence of human carcinoembryonic antigen material in blood
GB4418473A Expired GB1356912A (en) 1970-06-01 1971-06-01 Radioiodinated derivatives of carcinoembryonic antigen materials and processes for the production thereof
GB1830971*[A Expired GB1356911A (en) 1970-06-01 1971-06-01 Purification and fractionation of carcinoembryonic antigen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB4418773A Expired GB1358828A (en) 1970-06-01 1971-06-01 Process for detecting the presence of human carcinoembryonic antigen material or component a or b thereof in blood

Family Applications After (2)

Application Number Title Priority Date Filing Date
GB4418473A Expired GB1356912A (en) 1970-06-01 1971-06-01 Radioiodinated derivatives of carcinoembryonic antigen materials and processes for the production thereof
GB1830971*[A Expired GB1356911A (en) 1970-06-01 1971-06-01 Purification and fractionation of carcinoembryonic antigen

Country Status (13)

Country Link
US (1) US3697638A (en)
JP (1) JPS542247B1 (en)
BE (1) BE767801A (en)
CA (1) CA965351A (en)
CH (4) CH596554A5 (en)
DE (1) DE2126680B2 (en)
DK (1) DK132739C (en)
FR (1) FR2095644A5 (en)
GB (4) GB1358828A (en)
IL (1) IL36950A (en)
IT (1) IT998033B (en)
NL (1) NL161457C (en)
SE (5) SE400083B (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3867363A (en) * 1970-06-01 1975-02-18 Hans John Hansen Carcinoembryonic antigens
US3956258A (en) * 1972-05-12 1976-05-11 Hoffmann-La Roche Inc. Carcinoembryonic antigens
CH583036A5 (en) * 1972-05-12 1976-12-31 Hoffmann La Roche
ZA723264B (en) * 1972-10-13 1974-08-28 H Van Til Improvements in toys
US3867518A (en) * 1973-03-09 1975-02-18 Hoffmann La Roche Radioimmunoassay for insulin
US3927193A (en) * 1973-05-18 1975-12-16 Hoffmann La Roche Localization of tumors by radiolabelled antibodies
US4022876A (en) * 1973-06-21 1977-05-10 Stanford Research Institute Mass spectrometric immunoassay
US4132769A (en) * 1974-10-30 1979-01-02 Osther Kurt B Cancer antigen, cancer therapy, and cancer diagnosis
US4152410A (en) * 1975-09-03 1979-05-01 Eisai Co., Ltd. Diagnosis reagent for neoplasm and method for diagnosis of neoplasm
US4132767A (en) * 1976-04-05 1979-01-02 Eisai Co., Ltd. Preparation of α-L antibody, purification of α-L antigen and reagent for detection of α-L antibody and α-L antigen
US4144031A (en) * 1976-04-19 1979-03-13 International Radioimmune Systems, Inc. Cell test for detecting human chorionic gonadotropin
US4140753A (en) * 1976-04-30 1979-02-20 Scripps Clinic & Research Foundation Diagnostic method and reagent
US4145336A (en) * 1976-04-30 1979-03-20 Scripps Clinic And Research Foundation Carcinoembryonic antigen isomer
US4146603A (en) * 1977-02-18 1979-03-27 Research Corporation Tumor specific glycoproteins and method for detecting tumorigenic cancers
US4234476A (en) * 1978-03-07 1980-11-18 Research Corporation Application of protein-protein interaction as an assay for the detection of cancer
US4228127A (en) * 1978-10-06 1980-10-14 International Radioimmune Systems, Inc. Kit for detecting human chorionic gonadotropin
US4241044A (en) * 1978-12-28 1980-12-23 Abbott Laboratories Method of diagnosing cancer by detecting elevated anti-T antibody activity
US4349528A (en) * 1979-11-21 1982-09-14 The Wistar Institute Monocolonal hybridoma antibody specific for high molecular weight carcinoembryonic antigen
US4299815A (en) * 1980-02-08 1981-11-10 Hoffmann-La Roche Inc. Carcinoembryonic antigen determination
US4578349A (en) * 1982-04-08 1986-03-25 Abbott Laboratories Immunoassay for carcinoembryonic antigen (CEA)
DE3276968D1 (en) * 1982-08-09 1987-09-17 Centocor Inc Immunoassay for carbohydrate antigenic determinant
DK557483A (en) * 1982-12-06 1984-06-07 Hoffmann La Roche METHOD OF DETERMINING CEA
US4962187A (en) * 1984-11-28 1990-10-09 Cota Biotech Common antigen for colorectal and mucinous ovarian tumors and process for isolating the same
US5030559A (en) * 1986-04-01 1991-07-09 Board Of Regents, The University Of Texas System Methods and compositions for the identification of metastatic human tumors
US6013772A (en) * 1986-08-13 2000-01-11 Bayer Corporation Antibody preparations specifically binding to unique determinants of CEA antigens or fragments thereof and use of the antibody preparations in immunoassays
US5217903A (en) * 1990-05-15 1993-06-08 Trustees Of Boston University Measuring connective tissue breakdown products in body fluids

Also Published As

Publication number Publication date
SE397139B (en) 1977-10-17
SE419482B (en) 1981-08-03
CH595636A5 (en) 1978-02-15
DE2126680B2 (en) 1980-10-09
GB1356912A (en) 1974-06-19
GB1356911A (en) 1974-06-19
JPS542247B1 (en) 1979-02-05
DK132739C (en) 1976-07-12
CH604665A5 (en) 1978-09-15
IL36950A (en) 1975-04-25
CH606067A5 (en) 1978-10-13
SE400083B (en) 1978-03-13
US3697638A (en) 1972-10-10
CA965351A (en) 1975-04-01
BE767801A (en) 1971-11-29
FR2095644A5 (en) 1972-02-11
SE7413186L (en) 1974-10-18
NL7107499A (en) 1971-12-03
SE7413185L (en) 1974-10-18
SE421425B (en) 1981-12-21
GB1358828A (en) 1974-07-03
DK132739B (en) 1976-02-02
IL36950A0 (en) 1971-11-29
SE419481B (en) 1981-08-03
NL161457C (en) 1980-02-15
NL161457B (en) 1979-09-17
SE7413184L (en) 1974-10-18
CH596554A5 (en) 1978-03-15
DE2126680A1 (en) 1971-12-09
IT998033B (en) 1976-01-20
SE7413187L (en) 1974-10-18

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee