GB1273506A - Reagent for the determination of alkaline phosphatase in serum - Google Patents

Reagent for the determination of alkaline phosphatase in serum

Info

Publication number
GB1273506A
GB1273506A GB6901/70A GB690170A GB1273506A GB 1273506 A GB1273506 A GB 1273506A GB 6901/70 A GB6901/70 A GB 6901/70A GB 690170 A GB690170 A GB 690170A GB 1273506 A GB1273506 A GB 1273506A
Authority
GB
United Kingdom
Prior art keywords
reagent
determination
alkaline phosphatase
serum
extinction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB6901/70A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of GB1273506A publication Critical patent/GB1273506A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1,273,506. Determination of alkaline phosphatase. MERCK PATENT G.m.b.H. 12 Feb., 1970 [4 March, 1969], No. 6901/70. Heading B1X. A reagent for determination of alkaline phosphatase in serum comprises an aqueous solution of para-nitrophenyl phosphate containing diethanolamine hydrochloride in a molar concentration of at least 1.5 and at most 4.5. In an Example the reagent also contains sodium azide and magnesium chloride and is adjusted to a pH of 9.8. In carrying out a test the serum sample and the reagent are mixed and the rate of change of extinction of the mixture is measured at 405 nm.
GB6901/70A 1969-03-04 1970-02-12 Reagent for the determination of alkaline phosphatase in serum Expired GB1273506A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19691910803 DE1910803A1 (en) 1969-03-04 1969-03-04 Method and means for determining the level of alkaline phosphatase in serum

Publications (1)

Publication Number Publication Date
GB1273506A true GB1273506A (en) 1972-05-10

Family

ID=5726994

Family Applications (1)

Application Number Title Priority Date Filing Date
GB6901/70A Expired GB1273506A (en) 1969-03-04 1970-02-12 Reagent for the determination of alkaline phosphatase in serum

Country Status (9)

Country Link
BE (1) BE746829A (en)
BR (1) BR6915155D0 (en)
CH (1) CH535433A (en)
DE (1) DE1910803A1 (en)
FR (1) FR2037607A5 (en)
GB (1) GB1273506A (en)
IL (1) IL33898A (en)
NL (1) NL7003093A (en)
SE (1) SE360929B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114624205A (en) * 2022-02-28 2022-06-14 海南绿峰资源开发有限公司 Detection method of alkaline phosphatase

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA756003B (en) * 1975-09-22 1977-04-27 Chembro Holdings Pty Ltd Measurement of alkaline phophatase levels in body fluids

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114624205A (en) * 2022-02-28 2022-06-14 海南绿峰资源开发有限公司 Detection method of alkaline phosphatase

Also Published As

Publication number Publication date
SE360929B (en) 1973-10-08
FR2037607A5 (en) 1970-12-31
DE1910803A1 (en) 1970-10-08
NL7003093A (en) 1970-09-08
BE746829A (en) 1970-09-04
BR6915155D0 (en) 1973-02-08
IL33898A0 (en) 1970-04-20
CH535433A (en) 1973-03-31
IL33898A (en) 1972-12-29

Similar Documents

Publication Publication Date Title
GB1192046A (en) Laboratory Reagent for Assay of Urea
SG38686G (en) Process and diagnostic agents for detecting redox reactons
GB1489303A (en) Reagent and method for determining leukocytes and hemoglobin in the blood
ES485763A1 (en) Method and composition for direct determination of iron in blood serum
ES8106935A1 (en) Ascorbate resistant composition, test device and method for detecting a component in a liquid test sample.
GB1361149A (en) Reagent composition and method for determining calcium
GB1312712A (en) Method of determining bilirubin
ES352693A1 (en) Product for and method of testing blood for the presence of hemoglobin s
GB1288699A (en)
GB1371209A (en) Prostatic acid phosphatase determination
GB1273506A (en) Reagent for the determination of alkaline phosphatase in serum
YU167480A (en) Rapid test for escorbic acid assay
ES435761A1 (en) Radioisotopic assay and binder therefor
ES450075A1 (en) Stable dispersions of fluorescent whitening agents of the bis-triazinylaminostilbene group in free acid form and method of preparing same
GB1396219A (en) Method and reagent for testing blood
GB1386140A (en) Test composition and device
ES434127A1 (en) Determination of serum creatinine
GB1294518A (en) Test kit for use in the determination of uric acid in blood serum
GB1278036A (en) Diagnostic device
GB1424427A (en) Determination of calcium
GB1263202A (en) Alkaline phosphatase test
GB1164255A (en) Improvements in or relating to Diagnostic Package.
GB1531530A (en) Measurement of the anti-o-streptolysin antibody content of blood
GB769289A (en) Improvements in or relating to urine calcium test
GB1219624A (en) Colour reagents for the determination of creatinine

Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee