DE1648999C3 - Verfahren zur immunochemischen Bestimmung von Antigenen und Antikörpern - Google Patents
Verfahren zur immunochemischen Bestimmung von Antigenen und AntikörpernInfo
- Publication number
- DE1648999C3 DE1648999C3 DE1648999A DE1648999A DE1648999C3 DE 1648999 C3 DE1648999 C3 DE 1648999C3 DE 1648999 A DE1648999 A DE 1648999A DE 1648999 A DE1648999 A DE 1648999A DE 1648999 C3 DE1648999 C3 DE 1648999C3
- Authority
- DE
- Germany
- Prior art keywords
- reaction
- liquid
- drop
- determination
- components
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 26
- 239000000427 antigen Substances 0.000 title claims description 7
- 102000036639 antigens Human genes 0.000 title claims description 7
- 108091007433 antigens Proteins 0.000 title claims description 7
- 230000000984 immunochemical effect Effects 0.000 title claims description 5
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 22
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 22
- 238000006243 chemical reaction Methods 0.000 claims description 18
- 210000003743 erythrocyte Anatomy 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 17
- 239000000725 suspension Substances 0.000 claims description 16
- 210000002966 serum Anatomy 0.000 claims description 13
- 238000011534 incubation Methods 0.000 claims description 11
- 210000002700 urine Anatomy 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 8
- 239000004816 latex Substances 0.000 claims description 8
- 229920000126 latex Polymers 0.000 claims description 8
- 239000011541 reaction mixture Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000004062 sedimentation Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 4
- 102000018997 Growth Hormone Human genes 0.000 claims description 3
- 108010051696 Growth Hormone Proteins 0.000 claims description 3
- 239000000122 growth hormone Substances 0.000 claims description 3
- 230000035931 haemagglutination Effects 0.000 claims description 3
- 102000006771 Gonadotropins Human genes 0.000 claims description 2
- 108010086677 Gonadotropins Proteins 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- ZNOLGFHPUIJIMJ-UHFFFAOYSA-N fenitrothion Chemical compound COP(=S)(OC)OC1=CC=C([N+]([O-])=O)C(C)=C1 ZNOLGFHPUIJIMJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000002622 gonadotropin Substances 0.000 claims description 2
- 230000002265 prevention Effects 0.000 claims description 2
- 239000013049 sediment Substances 0.000 claims description 2
- 229920001864 tannin Polymers 0.000 claims description 2
- 235000018553 tannin Nutrition 0.000 claims description 2
- 239000001648 tannin Substances 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims 5
- 239000005556 hormone Substances 0.000 claims 5
- 102000009843 Thyroglobulin Human genes 0.000 claims 2
- 108010034949 Thyroglobulin Proteins 0.000 claims 2
- 229960002175 thyroglobulin Drugs 0.000 claims 2
- 208000001204 Hashimoto Disease Diseases 0.000 claims 1
- 208000030836 Hashimoto thyroiditis Diseases 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 claims 1
- 241000219061 Rheum Species 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 244000309464 bull Species 0.000 claims 1
- 210000003161 choroid Anatomy 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 238000005194 fractionation Methods 0.000 claims 1
- 108010074605 gamma-Globulins Proteins 0.000 claims 1
- 230000002452 interceptive effect Effects 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- 206010039073 rheumatoid arthritis Diseases 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- 238000001179 sorption measurement Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 20
- 102000009151 Luteinizing Hormone Human genes 0.000 description 12
- 108010073521 Luteinizing Hormone Proteins 0.000 description 12
- 229940040129 luteinizing hormone Drugs 0.000 description 12
- 239000008363 phosphate buffer Substances 0.000 description 12
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 108091006905 Human Serum Albumin Proteins 0.000 description 6
- 102000008100 Human Serum Albumin Human genes 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 108010071390 Serum Albumin Proteins 0.000 description 3
- 102000007562 Serum Albumin Human genes 0.000 description 3
- 230000004520 agglutination Effects 0.000 description 3
- 239000003708 ampul Substances 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000007979 citrate buffer Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 2
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 108010000521 Human Growth Hormone Proteins 0.000 description 1
- 239000000854 Human Growth Hormone Substances 0.000 description 1
- 206010062767 Hypophysitis Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000002175 menstrual effect Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 210000003635 pituitary gland Anatomy 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
- Y10S436/818—Human chorionic gonadotropin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Endocrinology (AREA)
- Mycology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL6615722 | 1966-11-08 | ||
| NL6708461A NL6708461A (enExample) | 1967-06-16 | 1967-06-16 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE1648999A1 DE1648999A1 (de) | 1971-08-12 |
| DE1648999B2 DE1648999B2 (de) | 1972-10-12 |
| DE1648999C3 true DE1648999C3 (de) | 1975-09-04 |
Family
ID=26644108
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE1648999A Expired DE1648999C3 (de) | 1966-11-08 | 1967-11-08 | Verfahren zur immunochemischen Bestimmung von Antigenen und Antikörpern |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US3565987A (enExample) |
| JP (1) | JPS4937241B1 (enExample) |
| BE (1) | BE706213A (enExample) |
| CA (1) | CA937498A (enExample) |
| CH (1) | CH512071A (enExample) |
| DE (1) | DE1648999C3 (enExample) |
| DK (1) | DK127200B (enExample) |
| ES (1) | ES346848A1 (enExample) |
| FI (1) | FI48510C (enExample) |
| GB (1) | GB1210819A (enExample) |
| NL (1) | NL130516C (enExample) |
| SE (1) | SE337494B (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USRE31006E (en) * | 1968-09-24 | 1982-08-03 | Akzona Incorporated | Process for the demonstration and determination of reaction components having specific binding affinity for each other |
| US3991174A (en) * | 1970-07-20 | 1976-11-09 | Rafa Laboratories Ltd. | Method of determining concentration of luteinizing hormone in body fluid |
| JPS49117244U (enExample) * | 1973-02-05 | 1974-10-07 | ||
| US4239746A (en) * | 1973-06-30 | 1980-12-16 | Dezso Istvan Bartos | Complement fixation test employing reactants in a disposable package |
| DE2333434C3 (de) * | 1973-06-30 | 1978-04-06 | Istvan D. Dr. 5024 Pulheim Bartos | Verfahren zur Durchführung serologischer Untersuchungen nach dem Prinzip der Komplementbindungsreaktion und gebrauchsfertige Schnelltestpackung hierfür |
| US3904367A (en) * | 1973-08-15 | 1975-09-09 | Gen Electric | Contrast enhancement for immunological film detection |
| JPS5079656U (enExample) * | 1973-11-27 | 1975-07-09 | ||
| JPS5247012B2 (enExample) * | 1974-11-16 | 1977-11-29 | ||
| US4003988A (en) * | 1976-06-01 | 1977-01-18 | Warner-Lambert Company | Direct agglutination test for pregnancy |
| US4092114A (en) * | 1976-10-20 | 1978-05-30 | Fisher Scientific Company | Indirect latex test for determination of immunoglobulins |
| US4289748A (en) * | 1979-05-31 | 1981-09-15 | United States Of America | Ultrasensitive enzymatic radioimmunoassay method |
| US4282002A (en) * | 1979-09-06 | 1981-08-04 | Akzona Incorporated | Sensitized sheep stroma immunoassay for rheumatoid factor |
| US4259207A (en) * | 1979-09-19 | 1981-03-31 | American Hospital Supply Corporation | Suspending medium for immunologic reactions |
| EP0039195B1 (en) * | 1980-04-28 | 1986-06-18 | Montefiore Hospital and Medical Center | Antibody detection process |
| FR2486657A1 (fr) * | 1980-07-09 | 1982-01-15 | Pasteur Institut | Procede de detection et de dosage d'une substance biologique par erythroadsorption |
| JPS6086468A (ja) * | 1983-10-18 | 1985-05-16 | Olympus Optical Co Ltd | 抗原抗体反応の判定方法 |
| JPS61170442U (enExample) * | 1985-04-13 | 1986-10-22 | ||
| JPS6223656U (enExample) * | 1985-07-25 | 1987-02-13 | ||
| US5597709A (en) * | 1994-01-27 | 1997-01-28 | Human Genome Sciences, Inc. | Human growth hormone splice variants hGHV-2(88) and hGHV-3(53) |
| CA3078625C (en) | 2017-10-09 | 2023-01-17 | Terumo Bct Biotechnologies, Llc | Lyophilization container and method of using same |
| PT110680A (pt) * | 2018-04-11 | 2019-10-11 | Inst Politecnico De Coimbra | Reagente para a pesquisa e/ou titulação do anticorpo anti-forssman. |
| CA3224729A1 (en) | 2019-03-14 | 2020-09-17 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
-
0
- NL NL130516D patent/NL130516C/xx active
-
1967
- 1967-10-20 US US676726A patent/US3565987A/en not_active Expired - Lifetime
- 1967-10-25 CA CA003389A patent/CA937498A/en not_active Expired
- 1967-10-30 GB GB49118/67A patent/GB1210819A/en not_active Expired
- 1967-11-01 FI FI672944A patent/FI48510C/fi active
- 1967-11-06 CH CH1548367A patent/CH512071A/de not_active IP Right Cessation
- 1967-11-07 SE SE15220/67A patent/SE337494B/xx unknown
- 1967-11-07 ES ES346848A patent/ES346848A1/es not_active Expired
- 1967-11-08 BE BE706213D patent/BE706213A/xx unknown
- 1967-11-08 JP JP42071469A patent/JPS4937241B1/ja active Pending
- 1967-11-08 DK DK556467AA patent/DK127200B/da unknown
- 1967-11-08 DE DE1648999A patent/DE1648999C3/de not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DE1648999A1 (de) | 1971-08-12 |
| GB1210819A (en) | 1970-11-04 |
| JPS4937241B1 (enExample) | 1974-10-07 |
| ES346848A1 (es) | 1969-03-16 |
| US3565987A (en) | 1971-02-23 |
| DE1648999B2 (de) | 1972-10-12 |
| BE706213A (enExample) | 1968-05-08 |
| FI48510C (fi) | 1974-10-10 |
| FI48510B (enExample) | 1974-07-01 |
| CA937498A (en) | 1973-11-27 |
| SE337494B (enExample) | 1971-08-09 |
| NL130516C (enExample) | |
| DK127200B (da) | 1973-10-01 |
| CH512071A (de) | 1971-08-31 |
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| CH647869A5 (de) | Verfahren zur bestimmung eines freien chemischen stoffes. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C3 | Grant after two publication steps (3rd publication) | ||
| E77 | Valid patent as to the heymanns-index 1977 | ||
| EHJ | Ceased/non-payment of the annual fee |