CZ300084B6 - Molekuly nukleové kyseliny kódující fruktosyltransferázu, vektor, hostitelská bunka, zpusob její prípravy a použití, rostlinná bunka, rostlina, zpusob prípravy rostliny, rozmnožovací materiál, skliditelné cásti rostliny, fruktosyltransferáza, zpusob - Google Patents
Molekuly nukleové kyseliny kódující fruktosyltransferázu, vektor, hostitelská bunka, zpusob její prípravy a použití, rostlinná bunka, rostlina, zpusob prípravy rostliny, rozmnožovací materiál, skliditelné cásti rostliny, fruktosyltransferáza, zpusob Download PDFInfo
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| DE19840028A1 (de) | 1998-09-02 | 2000-03-09 | Max Planck Gesellschaft | Nucleinsäuremoleküle codierend Enzyme, die Fructosyltransferaseaktivität besitzen, und deren Verwendung |
| DE19857654A1 (de) | 1998-12-14 | 2000-06-15 | Max Planck Gesellschaft | Beeinflussung des Blühverhaltens von Pflanzen durch Expression Saccharose-spaltender Proteine |
| US6791015B2 (en) | 2000-10-30 | 2004-09-14 | E. I. Du Pont De Nemours And Company | Fructan biosynthetic enzymes |
| US20040086902A1 (en) * | 2000-12-21 | 2004-05-06 | Dirk Engels | Method for the production of polyfructans |
| US7364882B1 (en) | 2004-09-24 | 2008-04-29 | State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Portland State University | Enzymatic reduction of a nitrile containing compound to the corresponding amine |
| EA021367B1 (ru) | 2008-11-28 | 2015-06-30 | Кова Компани, Лтд. | Производное пиридин-3-карбоксамида |
| TWI744225B (zh) | 2015-02-27 | 2021-11-01 | 美商林伯士拉克許米公司 | 酪胺酸蛋白質激酶２（ｔｙｋ２）抑制劑及其用途 |
| CN109562106B (zh) | 2016-06-21 | 2023-03-21 | X4 制药有限公司 | Cxcr4抑制剂及其用途 |
| ES2987356T3 (es) | 2018-04-24 | 2024-11-14 | Merck Patent Gmbh | Compuestos antiproliferación y usos de los mismos |
| EP3670659A1 (en) | 2018-12-20 | 2020-06-24 | Abivax | Biomarkers, and uses in treatment of viral infections, inflammations, or cancer |
| EP4048788A4 (en) * | 2019-11-27 | 2023-11-29 | Revelations Biotech Pvt Ltd | NUCLEIC ACIDS, VECTORS, HOST CELLS AND METHODS FOR PRODUCING BEETA-FRUCTOFURANOSIDASE FROM ASPERGILLUS NIGER |
| CN118745445B (zh) * | 2024-08-07 | 2025-02-28 | 苏州仁晟新材料科技有限公司 | 一种丙酮酸乙酯的制备方法 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0307158A2 (en) * | 1987-09-04 | 1989-03-15 | Nihon Shokuhin Kako Co., Ltd. | Process for the preparation of branched fructooligosaccharides |
| JPH04311378A (ja) * | 1990-03-16 | 1992-11-04 | Ajinomoto Co Inc | ポリフラクタンを含有する飲料 |
| WO1994014970A1 (en) * | 1992-12-28 | 1994-07-07 | Stichting Scheikundig Onderzoek In Nederland | Method for obtaining transgenic plants showing a modified fructan pattern |
| DE19617687A1 (de) * | 1996-05-03 | 1997-11-06 | Suedzucker Ag | Verfahren zur Herstellung transgener, Inulin erzeugender Pflanzen |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU682727B2 (en) * | 1993-12-23 | 1997-10-16 | Centro De Ingenieria Genetica Y Biotecnologia | Fructosyltransferase enzyme, method for its production, and DNA encoding the enzyme |
| EP1975237B1 (en) * | 1996-03-11 | 2012-12-26 | Meiji Co., Ltd. | Beta-fructofuranosidase variants and uses thereof |
| DE19619236A1 (de) | 1996-05-13 | 1997-11-20 | Bayer Ag | Abbau von biologisch abbaubaren Polyesteramiden mit Enzymen |
| DE19708774A1 (de) * | 1997-03-04 | 1998-09-17 | Max Planck Gesellschaft | Nucleinsäuremoleküle codierend Enzyme die Fructosylpolymeraseaktivität besitzen |
| CN1154720C (zh) * | 1997-09-10 | 2004-06-23 | 明治制果株式会社 | β-呋喃果糖苷酶及其基因 |
| DE19749122A1 (de) * | 1997-11-06 | 1999-06-10 | Max Planck Gesellschaft | Nucleinsäuremoleküle codierend Enzyme, die Fructosyltransferaseaktivität besitzen |
| DE19840028A1 (de) | 1998-09-02 | 2000-03-09 | Max Planck Gesellschaft | Nucleinsäuremoleküle codierend Enzyme, die Fructosyltransferaseaktivität besitzen, und deren Verwendung |
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1998
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1999
- 1999-08-27 CN CNB998105937A patent/CN1206357C/zh not_active Expired - Lifetime
- 1999-08-27 CZ CZ20010794A patent/CZ300084B6/cs not_active IP Right Cessation
- 1999-08-27 WO PCT/EP1999/006319 patent/WO2000014246A1/de not_active Ceased
- 1999-08-27 PL PL346422A patent/PL202383B1/pl not_active IP Right Cessation
- 1999-08-27 PT PT99968682T patent/PT1108037E/pt unknown
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- 1999-08-27 BR BR9913390-3A patent/BR9913390A/pt not_active Application Discontinuation
- 1999-08-27 HU HU0104121A patent/HU228010B1/hu not_active IP Right Cessation
- 1999-08-27 DK DK99968682T patent/DK1108037T3/da active
- 1999-08-27 EP EP99968682A patent/EP1108037B1/de not_active Expired - Lifetime
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0307158A2 (en) * | 1987-09-04 | 1989-03-15 | Nihon Shokuhin Kako Co., Ltd. | Process for the preparation of branched fructooligosaccharides |
| JPH04311378A (ja) * | 1990-03-16 | 1992-11-04 | Ajinomoto Co Inc | ポリフラクタンを含有する飲料 |
| WO1994014970A1 (en) * | 1992-12-28 | 1994-07-07 | Stichting Scheikundig Onderzoek In Nederland | Method for obtaining transgenic plants showing a modified fructan pattern |
| DE19617687A1 (de) * | 1996-05-03 | 1997-11-06 | Suedzucker Ag | Verfahren zur Herstellung transgener, Inulin erzeugender Pflanzen |
| WO1997042331A1 (de) * | 1996-05-03 | 1997-11-13 | Südzucker Aktiengesellschaft | Verfahren zur herstellung transgener, inulin erzeugender pflanzen |
Non-Patent Citations (1)
| Title |
|---|
| Somiari R.I. et al.: " Cloning and sequencing of an Aspergillus niger gene coding for beta-fructofuranosidase". Biotechnol. Lett. 19(12), 1243-1247, 1997 * |
Also Published As
| Publication number | Publication date |
|---|---|
| PL346422A1 (en) | 2002-02-11 |
| HUP0104121A2 (hu) | 2002-03-28 |
| CN1317048A (zh) | 2001-10-10 |
| HUP0104121A3 (en) | 2005-11-28 |
| JP2002524079A (ja) | 2002-08-06 |
| US20100011461A1 (en) | 2010-01-14 |
| CN1206357C (zh) | 2005-06-15 |
| JP4659983B2 (ja) | 2011-03-30 |
| AR021472A1 (es) | 2002-07-24 |
| US7906707B2 (en) | 2011-03-15 |
| CZ2001794A3 (cs) | 2002-01-16 |
| US6872555B2 (en) | 2005-03-29 |
| AU5971499A (en) | 2000-03-27 |
| BR9913390A (pt) | 2001-05-22 |
| EP1108037A1 (de) | 2001-06-20 |
| EP1108037B1 (de) | 2008-10-22 |
| PT1108037E (pt) | 2008-11-03 |
| US20050278806A1 (en) | 2005-12-15 |
| ATE412056T1 (de) | 2008-11-15 |
| DE59914884D1 (de) | 2008-12-04 |
| CA2342115C (en) | 2013-02-26 |
| CA2342115A1 (en) | 2000-03-16 |
| US7588922B2 (en) | 2009-09-15 |
| ZA200101737B (en) | 2002-01-02 |
| CY1109797T1 (el) | 2014-09-10 |
| HU228010B1 (hu) | 2012-08-28 |
| ES2312225T3 (es) | 2009-02-16 |
| WO2000014246A1 (de) | 2000-03-16 |
| PL202383B1 (pl) | 2009-06-30 |
| DK1108037T3 (da) | 2009-02-16 |
| AU764741B2 (en) | 2003-08-28 |
| US20020064850A1 (en) | 2002-05-30 |
| DE19840028A1 (de) | 2000-03-09 |
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