CY1115788T1 - Μεθοδοι για παραγωγη παρασκευασματων ανασυνδυασμενων aav ιοσωματιων ουσιαστικα απαλλαγμενων απο κενα καψιδια - Google Patents

Μεθοδοι για παραγωγη παρασκευασματων ανασυνδυασμενων aav ιοσωματιων ουσιαστικα απαλλαγμενων απο κενα καψιδια

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Publication number
CY1115788T1
CY1115788T1 CY20141100981T CY141100981T CY1115788T1 CY 1115788 T1 CY1115788 T1 CY 1115788T1 CY 20141100981 T CY20141100981 T CY 20141100981T CY 141100981 T CY141100981 T CY 141100981T CY 1115788 T1 CY1115788 T1 CY 1115788T1
Authority
CY
Cyprus
Prior art keywords
aav
methods
issomatic
recoverable
preparatory
Prior art date
Application number
CY20141100981T
Other languages
English (en)
Inventor
Guang Qu
John Fraser Wright
Original Assignee
Genzyme Corporation
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=33544272&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1115788(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corporation filed Critical Genzyme Corporation
Publication of CY1115788T1 publication Critical patent/CY1115788T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16651—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material

Abstract

Μέθοδοι για διαχωρισμό AAV κενών καψιδίων από μείγματα σωματιδίων AAV φορέα και AAV κενών καψιδίων περιγράφονται. Οι μέθοδοι χρησιμοποιούν τεχνικές χρωματογραφίας στήλης και παρέχουν εμπορικώς βιώσιμα επίπεδα ανασυνδυασμένων AAV ιοσωμάτιων.
CY20141100981T 2003-05-21 2014-11-26 Μεθοδοι για παραγωγη παρασκευασματων ανασυνδυασμενων aav ιοσωματιων ουσιαστικα απαλλαγμενων απο κενα καψιδια CY1115788T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US47238403P 2003-05-21 2003-05-21
US54975604P 2004-03-02 2004-03-02
EP10179750.4A EP2277996B1 (en) 2003-05-21 2004-05-21 Methods for producing preparations of recombinant AAV virions substantially free of empty capsids

Publications (1)

Publication Number Publication Date
CY1115788T1 true CY1115788T1 (el) 2017-01-25

Family

ID=33544272

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20141100981T CY1115788T1 (el) 2003-05-21 2014-11-26 Μεθοδοι για παραγωγη παρασκευασματων ανασυνδυασμενων aav ιοσωματιων ουσιαστικα απαλλαγμενων απο κενα καψιδια

Country Status (12)

Country Link
US (7) US7261544B2 (el)
EP (4) EP2781596A1 (el)
JP (6) JP4559429B2 (el)
AT (1) ATE490307T1 (el)
CY (1) CY1115788T1 (el)
DE (1) DE602004030327D1 (el)
DK (1) DK2277996T3 (el)
ES (1) ES2521682T3 (el)
PL (2) PL2277996T3 (el)
PT (2) PT1625210E (el)
SI (1) SI2277996T1 (el)
WO (1) WO2004113494A2 (el)

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