CY1114747T1 - Χιμαιρα dna πολυμερασης φαγου φ 29 - Google Patents

Χιμαιρα dna πολυμερασης φαγου φ 29

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Publication number
CY1114747T1
CY1114747T1 CY20131101144T CY131101144T CY1114747T1 CY 1114747 T1 CY1114747 T1 CY 1114747T1 CY 20131101144 T CY20131101144 T CY 20131101144T CY 131101144 T CY131101144 T CY 131101144T CY 1114747 T1 CY1114747 T1 CY 1114747T1
Authority
CY
Cyprus
Prior art keywords
terminal
phage
chemistry
dna polymerase
dna
Prior art date
Application number
CY20131101144T
Other languages
English (en)
Inventor
Falgueras Margarita Salas
Vega José Miguel De
BOLOS JOSÉ Mª LAZARO
Davila Luis Blanco
Caballero Mario Mencia
Original Assignee
Consejo Superior De Investigaciones Cientificas (Csic)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior De Investigaciones Cientificas (Csic) filed Critical Consejo Superior De Investigaciones Cientificas (Csic)
Publication of CY1114747T1 publication Critical patent/CY1114747T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y599/00—Other isomerases (5.99)
    • C12Y599/01—Other isomerases (5.99.1)
    • C12Y599/01002—DNA topoisomerase (5.99.1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Abstract

Η παρούσα εφεύρεση περικλείεται στο πεδίο της βιοτεχνολογίας. Ειδικώς, αφορά μια χίμαιρα DΝΑ πολυμεράσης που αποτελείται από μια αμινο-τερματική (Ν-τερματική) περιοχή η οποία κωδικοποιεί μια DΝΑ πολυμεράση τύπου φ29 και μια καρβοξυλ-τερματική (C-τερματική) περιοχή η οποία περιλαμβάνει τουλάχιστον ένα πεδίο ΗhH που προδένονται μέσω μιας συνδετικής αλληλουχίας αμινοξέων και τη χρήση αυτών για την αντιγραφή, ενίσχυση ή αλληλούχηση ενός πρότυπου DΝΑ. Παρομοίως, η παρούσα εφεύρεση παρέχει μια μέθοδο για την αντιγραφή, ενίσχυση ή αλληλούχηση ενός δεοξυριβονουκλεϊνικού οξέος με την εν λόγω χίμαιρα DΝΑ πολυμεράσης και ένα κιτ για την εκτέλεση της εν λόγω μεθόδου.
CY20131101144T 2009-07-02 2013-12-18 Χιμαιρα dna πολυμερασης φαγου φ 29 CY1114747T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200930413A ES2359058B1 (es) 2009-07-02 2009-07-02 Quimera de adn polimerasa del fago ph1 29.
EP10793649.4A EP2450436B1 (en) 2009-07-02 2010-07-01 Phage phi 29 dna polymerase chimera

Publications (1)

Publication Number Publication Date
CY1114747T1 true CY1114747T1 (el) 2016-12-14

Family

ID=43410525

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20131101144T CY1114747T1 (el) 2009-07-02 2013-12-18 Χιμαιρα dna πολυμερασης φαγου φ 29

Country Status (24)

Country Link
US (2) US8404808B2 (el)
EP (1) EP2450436B1 (el)
JP (1) JP5778671B2 (el)
KR (1) KR101751554B1 (el)
CN (1) CN102648275B (el)
AU (1) AU2010267954B2 (el)
BR (1) BR112012000026A2 (el)
CA (1) CA2766725C (el)
CL (1) CL2011003312A1 (el)
CO (1) CO6480970A2 (el)
CY (1) CY1114747T1 (el)
DK (1) DK2450436T3 (el)
ES (2) ES2359058B1 (el)
HK (1) HK1173188A1 (el)
HR (1) HRP20131163T1 (el)
IL (1) IL217324A0 (el)
MX (1) MX2012000133A (el)
NZ (1) NZ597884A (el)
PL (1) PL2450436T3 (el)
PT (1) PT2450436E (el)
SG (1) SG177410A1 (el)
SI (1) SI2450436T1 (el)
SM (1) SMT201300153B (el)
WO (1) WO2011000997A1 (el)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103517993A (zh) * 2011-02-28 2014-01-15 环球基因有限公司 用于对混合群体中的靶dna进行测序的试剂盒和方法
WO2014140309A1 (en) 2013-03-15 2014-09-18 Sygnis Biotech S.L.U. Methods for amplification and sequencing using thermostable tthprimpol
US9447445B2 (en) * 2014-08-27 2016-09-20 New England Biolabs, Inc. Synthon formation
CN105483115A (zh) * 2014-09-19 2016-04-13 深圳华大基因科技有限公司 一种用于单细胞全基因组扩增的试剂盒及其应用
KR20230052995A (ko) * 2016-12-19 2023-04-20 퀀텀-에스아이 인코포레이티드 시퀀싱 반응을 위한 중합 효소
JP2022539560A (ja) 2019-06-28 2022-09-12 クアンタム－エスアイ インコーポレイテッド 配列決定反応のための重合酵素
CN110747191B (zh) * 2019-10-17 2021-08-17 深圳清华大学研究院 多肽、嵌合聚合酶及其应用
JP2023511991A (ja) 2020-01-31 2023-03-23 ティリス セラピューティクス，エセ．エレ． 閉じた直鎖ｄｎａを作製するためのプロセス
CA3164390A1 (en) 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Closed linear dna with modified nucleotides
EP4299731A1 (en) 2022-06-29 2024-01-03 ASOCIACIÓN CENTRO DE INVESTIGACIÓN COOPERATIVA EN NANOCIENCIAS "CIC nanoGUNE" Synthetic primase-polymerase and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US20060234227A1 (en) * 2002-05-14 2006-10-19 Alexei Slesarev Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes
CA2519309A1 (en) * 2003-03-25 2004-10-14 Stratagene California Dna polymerase fusions and uses thereof
JP5241493B2 (ja) * 2005-07-15 2013-07-17 アジレント・テクノロジーズ・インク Ｄｎａ結合タンパク質−ポリメラーゼのキメラ

Also Published As

Publication number Publication date
EP2450436B1 (en) 2013-10-02
CA2766725C (en) 2016-10-11
HK1173188A1 (zh) 2013-05-10
CL2011003312A1 (es) 2012-06-01
US20120190014A1 (en) 2012-07-26
HRP20131163T1 (hr) 2014-02-14
ES2359058B1 (es) 2012-03-27
KR101751554B1 (ko) 2017-06-28
CO6480970A2 (es) 2012-07-16
DK2450436T3 (da) 2014-01-13
CN102648275A (zh) 2012-08-22
KR20120052954A (ko) 2012-05-24
SG177410A1 (en) 2012-02-28
BR112012000026A2 (pt) 2015-09-08
PT2450436E (pt) 2013-12-27
US8404808B2 (en) 2013-03-26
WO2011000997A1 (es) 2011-01-06
EP2450436A4 (en) 2012-11-28
NZ597884A (en) 2013-11-29
EP2450436A1 (en) 2012-05-09
CN102648275B (zh) 2015-11-25
CA2766725A1 (en) 2011-01-06
JP2012531220A (ja) 2012-12-10
AU2010267954A1 (en) 2012-02-16
IL217324A0 (en) 2012-02-29
US20130236886A1 (en) 2013-09-12
PL2450436T3 (pl) 2014-03-31
SMT201300153B (it) 2014-03-07
ES2438786T3 (es) 2014-01-20
JP5778671B2 (ja) 2015-09-16
MX2012000133A (es) 2012-04-30
SI2450436T1 (sl) 2014-02-28
AU2010267954B2 (en) 2014-12-11
ES2359058A1 (es) 2011-05-18

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