SMT201300153B - Chimera di dna polimerasi del fago phi29 - Google Patents

Chimera di dna polimerasi del fago phi29

Info

Publication number
SMT201300153B
SMT201300153B SM201300153T SM201300153T SMT201300153B SM T201300153 B SMT201300153 B SM T201300153B SM 201300153 T SM201300153 T SM 201300153T SM 201300153 T SM201300153 T SM 201300153T SM T201300153 B SMT201300153 B SM T201300153B
Authority
SM
San Marino
Prior art keywords
chimera
phage phi29
dna polymeric
dna
polymeric
Prior art date
Application number
SM201300153T
Other languages
English (en)
Italian (it)
Inventor
Falgueras Margarita Salas
Vega Jose Miguel De
Bolos Jose M Lazaro
Davila Luis Blanco
Caballero Mario Mencia
Original Assignee
Consejo Superior Investigacion
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior Investigacion filed Critical Consejo Superior Investigacion
Publication of SMT201300153B publication Critical patent/SMT201300153B/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01DNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07007DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y599/00Other isomerases (5.99)
    • C12Y599/01Other isomerases (5.99.1)
    • C12Y599/01002DNA topoisomerase (5.99.1.2)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
SM201300153T 2009-07-02 2013-12-19 Chimera di dna polimerasi del fago phi29 SMT201300153B (it)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200930413A ES2359058B1 (es) 2009-07-02 2009-07-02 Quimera de adn polimerasa del fago ph1 29.
PCT/ES2010/070454 WO2011000997A1 (es) 2009-07-02 2010-07-01 QUIMERA DE ADN POLIMERASA DEL FAGO φ 29

Publications (1)

Publication Number Publication Date
SMT201300153B true SMT201300153B (it) 2014-03-07

Family

ID=43410525

Family Applications (1)

Application Number Title Priority Date Filing Date
SM201300153T SMT201300153B (it) 2009-07-02 2013-12-19 Chimera di dna polimerasi del fago phi29

Country Status (24)

Country Link
US (2) US8404808B2 (el)
EP (1) EP2450436B1 (el)
JP (1) JP5778671B2 (el)
KR (1) KR101751554B1 (el)
CN (1) CN102648275B (el)
AU (1) AU2010267954B2 (el)
BR (1) BR112012000026A2 (el)
CA (1) CA2766725C (el)
CL (1) CL2011003312A1 (el)
CO (1) CO6480970A2 (el)
CY (1) CY1114747T1 (el)
DK (1) DK2450436T3 (el)
ES (2) ES2359058B1 (el)
HK (1) HK1173188A1 (el)
HR (1) HRP20131163T1 (el)
IL (1) IL217324A0 (el)
MX (1) MX2012000133A (el)
NZ (1) NZ597884A (el)
PL (1) PL2450436T3 (el)
PT (1) PT2450436E (el)
SG (1) SG177410A1 (el)
SI (1) SI2450436T1 (el)
SM (1) SMT201300153B (el)
WO (1) WO2011000997A1 (el)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103517993A (zh) * 2011-02-28 2014-01-15 环球基因有限公司 用于对混合群体中的靶dna进行测序的试剂盒和方法
CN105283558B (zh) 2013-03-15 2019-05-10 西格尼斯生物技术有限责任公司 使用热稳定的TthPrimPol扩增和测序的方法
CN107075511B9 (zh) * 2014-08-27 2021-10-26 新英格兰生物实验室公司 合成子的形成
CN105483115A (zh) * 2014-09-19 2016-04-13 深圳华大基因科技有限公司 一种用于单细胞全基因组扩增的试剂盒及其应用
JP2020501573A (ja) * 2016-12-19 2020-01-23 クアンタム−エスアイ インコーポレイテッドQuantum−Si Incorporated シークエンシング反応用の重合酵素
EP3976771A1 (en) 2019-06-28 2022-04-06 Quantum-si Incorporated Polymerizing enzymes for sequencing reactions
CN110747191B (zh) * 2019-10-17 2021-08-17 深圳清华大学研究院 多肽、嵌合聚合酶及其应用
WO2021152147A1 (en) 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Closed linear dna with modified nucleotides
WO2021152146A1 (en) 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Process for the production of closed linear dna
EP4299731A1 (en) 2022-06-29 2024-01-03 ASOCIACIÓN CENTRO DE INVESTIGACIÓN COOPERATIVA EN NANOCIENCIAS "CIC nanoGUNE" Synthetic primase-polymerase and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
WO2004013279A2 (en) * 2002-05-14 2004-02-12 Fidelity Systems, Inc. Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes
WO2004087868A2 (en) * 2003-03-25 2004-10-14 Stratagene Dna polymerase fusions and uses thereof
WO2007011891A2 (en) 2005-07-15 2007-01-25 Strategene California Dna binding protein-polymerase chimeras

Also Published As

Publication number Publication date
HRP20131163T1 (hr) 2014-02-14
KR20120052954A (ko) 2012-05-24
JP2012531220A (ja) 2012-12-10
AU2010267954A1 (en) 2012-02-16
ES2438786T3 (es) 2014-01-20
DK2450436T3 (da) 2014-01-13
US8404808B2 (en) 2013-03-26
US20130236886A1 (en) 2013-09-12
HK1173188A1 (zh) 2013-05-10
NZ597884A (en) 2013-11-29
CO6480970A2 (es) 2012-07-16
US20120190014A1 (en) 2012-07-26
SI2450436T1 (sl) 2014-02-28
CY1114747T1 (el) 2016-12-14
CA2766725C (en) 2016-10-11
CN102648275A (zh) 2012-08-22
PL2450436T3 (pl) 2014-03-31
AU2010267954B2 (en) 2014-12-11
CA2766725A1 (en) 2011-01-06
IL217324A0 (en) 2012-02-29
MX2012000133A (es) 2012-04-30
EP2450436A4 (en) 2012-11-28
ES2359058A1 (es) 2011-05-18
BR112012000026A2 (pt) 2015-09-08
PT2450436E (pt) 2013-12-27
JP5778671B2 (ja) 2015-09-16
KR101751554B1 (ko) 2017-06-28
SG177410A1 (en) 2012-02-28
CL2011003312A1 (es) 2012-06-01
WO2011000997A1 (es) 2011-01-06
ES2359058B1 (es) 2012-03-27
EP2450436A1 (en) 2012-05-09
EP2450436B1 (en) 2013-10-02
CN102648275B (zh) 2015-11-25

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