MX2012000133A - Quimera de adn polimerasa del fago phi29. - Google Patents
Quimera de adn polimerasa del fago phi29.Info
- Publication number
- MX2012000133A MX2012000133A MX2012000133A MX2012000133A MX2012000133A MX 2012000133 A MX2012000133 A MX 2012000133A MX 2012000133 A MX2012000133 A MX 2012000133A MX 2012000133 A MX2012000133 A MX 2012000133A MX 2012000133 A MX2012000133 A MX 2012000133A
- Authority
- MX
- Mexico
- Prior art keywords
- dna polymerase
- terminal
- phage
- polymerase chimera
- chimera
- Prior art date
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 title abstract 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 title abstract 4
- 230000003321 amplification Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 230000010076 replication Effects 0.000 abstract 2
- 238000012163 sequencing technique Methods 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 210000004899 c-terminal region Anatomy 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/01—DNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y599/00—Other isomerases (5.99)
- C12Y599/01—Other isomerases (5.99.1)
- C12Y599/01002—DNA topoisomerase (5.99.1.2)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Abstract
La presente invención se encuadra dentro del campo de la biotecnología. Específicamente, se refiere a una quimera de ADN polimerasa que comprende una región amino-terminal (N-terminal), que codifica para una ADN polimerasa del tipo 29, y una región carboxilo-terminal (C-terminal), que comprende, al menos, un dominio HhH, que se encuentran unidas mediante una secuencia aminoacídica conectora y a su uso para la replicación, amplificación o secuenciación de un ADN molde. Asimismo, la presente invención proporciona un método para llevar a cabo la replicación, la amplificación o la secuenciación de un ácido desoxirribonucleico con la quimera de ADN polimerasa y un kit para llevar a cabo el método.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200930413A ES2359058B1 (es) | 2009-07-02 | 2009-07-02 | Quimera de adn polimerasa del fago ph1 29. |
| PCT/ES2010/070454 WO2011000997A1 (es) | 2009-07-02 | 2010-07-01 | QUIMERA DE ADN POLIMERASA DEL FAGO φ 29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2012000133A true MX2012000133A (es) | 2012-04-30 |
Family
ID=43410525
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2012000133A MX2012000133A (es) | 2009-07-02 | 2010-07-01 | Quimera de adn polimerasa del fago phi29. |
Country Status (24)
| Country | Link |
|---|---|
| US (2) | US8404808B2 (es) |
| EP (1) | EP2450436B1 (es) |
| JP (1) | JP5778671B2 (es) |
| KR (1) | KR101751554B1 (es) |
| CN (1) | CN102648275B (es) |
| AU (1) | AU2010267954B2 (es) |
| BR (1) | BR112012000026A2 (es) |
| CA (1) | CA2766725C (es) |
| CL (1) | CL2011003312A1 (es) |
| CO (1) | CO6480970A2 (es) |
| CY (1) | CY1114747T1 (es) |
| DK (1) | DK2450436T3 (es) |
| ES (2) | ES2359058B1 (es) |
| HK (1) | HK1173188A1 (es) |
| HR (1) | HRP20131163T1 (es) |
| IL (1) | IL217324A0 (es) |
| MX (1) | MX2012000133A (es) |
| NZ (1) | NZ597884A (es) |
| PL (1) | PL2450436T3 (es) |
| PT (1) | PT2450436E (es) |
| SG (1) | SG177410A1 (es) |
| SI (1) | SI2450436T1 (es) |
| SM (1) | SMT201300153B (es) |
| WO (1) | WO2011000997A1 (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012223438A1 (en) * | 2011-02-28 | 2013-09-26 | Transgenomic, Inc | Kit and method for sequencing a target DNA in a mixed population |
| EP2971080B1 (en) | 2013-03-15 | 2018-01-03 | Expedeon, S.L. | Methods for amplification and sequencing using thermostable tthprimpol |
| US9447445B2 (en) * | 2014-08-27 | 2016-09-20 | New England Biolabs, Inc. | Synthon formation |
| CN105483115A (zh) * | 2014-09-19 | 2016-04-13 | 深圳华大基因科技有限公司 | 一种用于单细胞全基因组扩增的试剂盒及其应用 |
| JP2020501573A (ja) * | 2016-12-19 | 2020-01-23 | クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ | シークエンシング反応用の重合酵素 |
| US11959105B2 (en) | 2019-06-28 | 2024-04-16 | Quantum-Si Incorporated | Polymerizing enzymes for sequencing reactions |
| CN110747191B (zh) * | 2019-10-17 | 2021-08-17 | 深圳清华大学研究院 | 多肽、嵌合聚合酶及其应用 |
| JP2023511991A (ja) | 2020-01-31 | 2023-03-23 | ティリス セラピューティクス，エセ．エレ． | 閉じた直鎖ｄｎａを作製するためのプロセス |
| JP2023511992A (ja) | 2020-01-31 | 2023-03-23 | ティリス セラピューティクス，エセ．エレ． | 修飾ヌクレオチドを用いた閉じた直鎖ｄｎａ |
| EP4299731A1 (en) | 2022-06-29 | 2024-01-03 | ASOCIACIÓN CENTRO DE INVESTIGACIÓN COOPERATIVA EN NANOCIENCIAS "CIC nanoGUNE" | Synthetic primase-polymerase and uses thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5001050A (en) | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
| US5198543A (en) | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| US6323009B1 (en) | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
| WO2004013279A2 (en) * | 2002-05-14 | 2004-02-12 | Fidelity Systems, Inc. | Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes |
| AU2004225520A1 (en) * | 2003-03-25 | 2004-10-14 | Stratagene | DNA polymerase fusions and uses thereof |
| WO2007011891A2 (en) | 2005-07-15 | 2007-01-25 | Strategene California | Dna binding protein-polymerase chimeras |
-
2009
- 2009-07-02 ES ES200930413A patent/ES2359058B1/es not_active Expired - Fee Related
-
2010
- 2010-07-01 KR KR1020127002884A patent/KR101751554B1/ko active IP Right Grant
- 2010-07-01 EP EP10793649.4A patent/EP2450436B1/en active Active
- 2010-07-01 CN CN201080039946.9A patent/CN102648275B/zh active Active
- 2010-07-01 NZ NZ597884A patent/NZ597884A/xx not_active IP Right Cessation
- 2010-07-01 DK DK10793649.4T patent/DK2450436T3/da active
- 2010-07-01 JP JP2012519025A patent/JP5778671B2/ja active Active
- 2010-07-01 PL PL10793649T patent/PL2450436T3/pl unknown
- 2010-07-01 AU AU2010267954A patent/AU2010267954B2/en active Active
- 2010-07-01 PT PT107936494T patent/PT2450436E/pt unknown
- 2010-07-01 CA CA2766725A patent/CA2766725C/en active Active
- 2010-07-01 SI SI201030435T patent/SI2450436T1/sl unknown
- 2010-07-01 MX MX2012000133A patent/MX2012000133A/es active IP Right Grant
- 2010-07-01 WO PCT/ES2010/070454 patent/WO2011000997A1/es active Application Filing
- 2010-07-01 SG SG2011097060A patent/SG177410A1/en unknown
- 2010-07-01 US US13/381,837 patent/US8404808B2/en active Active
- 2010-07-01 BR BR112012000026A patent/BR112012000026A2/pt not_active IP Right Cessation
- 2010-07-01 ES ES10793649.4T patent/ES2438786T3/es active Active
-
2011
- 2011-12-26 CL CL2011003312A patent/CL2011003312A1/es unknown
- 2011-12-29 CO CO11180682A patent/CO6480970A2/es active IP Right Grant
-
2012
- 2012-01-02 IL IL217324A patent/IL217324A0/en unknown
-
2013
- 2013-01-11 HK HK13100537.5A patent/HK1173188A1/zh not_active IP Right Cessation
- 2013-03-15 US US13/837,944 patent/US20130236886A1/en not_active Abandoned
- 2013-12-05 HR HRP20131163AT patent/HRP20131163T1/hr unknown
- 2013-12-18 CY CY20131101144T patent/CY1114747T1/el unknown
- 2013-12-19 SM SM201300153T patent/SMT201300153B/xx unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |