MX2010000232A - Metodos mejorados para la formacion de enlaces disulfuro. - Google Patents

Metodos mejorados para la formacion de enlaces disulfuro.

Info

Publication number
MX2010000232A
MX2010000232A MX2010000232A MX2010000232A MX2010000232A MX 2010000232 A MX2010000232 A MX 2010000232A MX 2010000232 A MX2010000232 A MX 2010000232A MX 2010000232 A MX2010000232 A MX 2010000232A MX 2010000232 A MX2010000232 A MX 2010000232A
Authority
MX
Mexico
Prior art keywords
formation
disulphide bonds
improved methods
methods
peptides
Prior art date
Application number
MX2010000232A
Other languages
English (en)
Inventor
Josef Prassler
Yvonne Stark
Original Assignee
Morphosys Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morphosys Ag filed Critical Morphosys Ag
Publication of MX2010000232A publication Critical patent/MX2010000232A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a métodos para la formación de enlaces disulfuro intermoleculares, que incluyen (poli)péptidos/proteínas, ácidos nucleicos, vectores, células hospederas y bacteriófagos usados en estos métodos. Además, la invención se refiere al uso de este método para el despliegue mejorado de (poli) péptidos/proteínas en la superficie de las partículas del bacteriófago.
MX2010000232A 2007-08-21 2008-08-21 Metodos mejorados para la formacion de enlaces disulfuro. MX2010000232A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95702707P 2007-08-21 2007-08-21
US98903507P 2007-11-19 2007-11-19
PCT/EP2008/060931 WO2009024593A1 (en) 2007-08-21 2008-08-21 Improved methods for the formation of disulphide bonds

Publications (1)

Publication Number Publication Date
MX2010000232A true MX2010000232A (es) 2010-06-02

Family

ID=39929757

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010000232A MX2010000232A (es) 2007-08-21 2008-08-21 Metodos mejorados para la formacion de enlaces disulfuro.

Country Status (14)

Country Link
US (1) US9062097B2 (es)
EP (1) EP2190987B1 (es)
JP (2) JP2011504722A (es)
KR (1) KR20100089816A (es)
CN (1) CN101772574B (es)
AU (1) AU2008290539B2 (es)
CA (1) CA2694364C (es)
DK (1) DK2190987T3 (es)
ES (1) ES2403904T3 (es)
IL (1) IL203093A0 (es)
MX (1) MX2010000232A (es)
RU (1) RU2010102859A (es)
WO (1) WO2009024593A1 (es)
ZA (1) ZA201000975B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2694364C (en) 2007-08-21 2016-11-22 Morphosys Ag Improved methods for the formation of disulphide bonds
DK2435568T3 (da) 2009-05-29 2014-09-08 Morphosys Ag Samling af syntetiske antistoffer til behandling af sygdom
SG10201509257VA (en) 2010-11-19 2015-12-30 Morphosys Ag A collection and methods for its use
CN107513104A (zh) * 2011-01-28 2017-12-26 加拿大国家研究委员会 免疫球蛋白结构域的工程改造
US11453883B2 (en) 2018-04-05 2022-09-27 Bio-Rad Abd Serotec Gmbh Display systems for proteins of interest

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US413276A (en) * 1889-10-22 Central-station calling apparatus
DE3852304T3 (de) 1987-03-02 1999-07-01 Enzon Lab Inc Organismus als Träger für "Single Chain Antibody Domain (SCAD)".
JP3771253B2 (ja) 1988-09-02 2006-04-26 ダイアックス コープ． 新規な結合タンパク質の生成と選択
US5545727A (en) * 1989-05-10 1996-08-13 Somatogen, Inc. DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin
US5747334A (en) 1990-02-15 1998-05-05 The University Of North Carolina At Chapel Hill Random peptide library
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5723286A (en) 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US5770434A (en) 1990-09-28 1998-06-23 Ixsys Incorporated Soluble peptides having constrained, secondary conformation in solution and method of making same
US5871974A (en) 1990-09-28 1999-02-16 Ixsys Inc. Surface expression libraries of heteromeric receptors
DK0564531T3 (da) 1990-12-03 1998-09-28 Genentech Inc Berigelsesfremgangsmåde for variantproteiner med ændrede bindingsegenskaber
GB9114003D0 (en) 1991-06-28 1991-08-14 Mastico Robert A Chimaeric protein
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US6025165A (en) 1991-11-25 2000-02-15 Enzon, Inc. Methods for producing multivalent antigen-binding proteins
GB9213601D0 (en) 1992-06-26 1992-08-12 Mastico Robert A Protein based delivery system
DE614989T1 (de) 1993-02-17 1995-09-28 Morphosys Proteinoptimierung Verfahren für in vivo Selektion von Ligandenbindende Proteine.
GB9313509D0 (en) 1993-06-30 1993-08-11 Medical Res Council Chemisynthetic libraries
US6309642B1 (en) 1997-04-28 2001-10-30 The Research And Development Institute, Inc. Peptides which mimic candida carbohydrate epitopes and their use in a vaccine
US5945311A (en) 1994-06-03 1999-08-31 GSF--Forschungszentrumfur Umweltund Gesundheit Method for producing heterologous bi-specific antibodies
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US6341256B1 (en) 1995-03-31 2002-01-22 Curagen Corporation Consensus configurational bias Monte Carlo method and system for pharmacophore structure determination
US6017754A (en) 1995-08-24 2000-01-25 Invitrogen Corporation System for isolating and identifying eukaryotic cells transfected with genes and vectors, host cells and methods thereof
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6420140B1 (en) 1996-10-11 2002-07-16 Abgenix, Inc. Production of multimeric protein by cell fusion method
US20020062010A1 (en) 1997-05-02 2002-05-23 Genentech, Inc. Method for making multispecific antibodies having heteromultimeric and common components
WO1999006587A2 (en) 1997-08-01 1999-02-11 Morphosys Ag Novel method and phage for the identification of nucleic acid sequences encoding members of a multimeric (poly)peptide complex
US6046166A (en) * 1997-09-29 2000-04-04 Jean-Louis Dasseux Apolipoprotein A-I agonists and their use to treat dyslipidemic disorders
JP4493846B2 (ja) 1998-05-13 2010-06-30 ドマンティス リミテッド 選択系
US6962702B2 (en) 1998-06-22 2005-11-08 Immunomedics Inc. Production and use of novel peptide-based agents for use with bi-specific antibodies
US7052872B1 (en) 1999-06-22 2006-05-30 Immunomedics, Inc. Bi-specific antibodies for pre-targeting diagnosis and therapy
IL142025A0 (en) 1999-07-20 2002-03-10 Morphosys Ag Novel methods for displaying (poly) peptides/proteins on bacteriophage particles via disulfide bonds
EP2357187A1 (en) 2000-12-12 2011-08-17 MedImmune, LLC Molecules with extended half-lives, compositions and uses thereof
US6833441B2 (en) 2001-08-01 2004-12-21 Abmaxis, Inc. Compositions and methods for generating chimeric heteromultimers
AU2002330162B2 (en) 2001-10-01 2008-08-07 Takeda Pharmaceutical Company Limited Multi-chain eukaryotic display vectors and uses thereof
DE50115580D1 (de) 2001-10-01 2010-09-16 Deutsches Krebsforsch Verfahren zur Herstellung von Protein-Bibliotheken und zur Selektion von Proteinen daraus
US7175983B2 (en) 2001-11-02 2007-02-13 Abmaxis, Inc. Adapter-directed display systems
US8206977B2 (en) 2002-07-30 2012-06-26 Morphosys Ag Tricistronic vectors and uses therefor
WO2005021710A2 (en) 2003-06-02 2005-03-10 University Of Miami Chimeric molecules and methods of use
WO2005063817A2 (en) 2003-12-22 2005-07-14 Amgen Inc. Methods for identifying functional antibodies
KR20070007793A (ko) 2004-02-05 2007-01-16 메사추세츠 인스티튜트 오브 테크놀로지 세포 디스플레이 라이브러리
EP2053062A1 (en) 2004-03-24 2009-04-29 Xencor, Inc. Immunoglobin variants outside the Fc region
AU2005282700A1 (en) 2004-09-02 2006-03-16 Genentech, Inc. Heteromultimeric molecules
TW200732350A (en) 2005-10-21 2007-09-01 Amgen Inc Methods for generating monovalent IgG
JP5525729B2 (ja) 2005-11-28 2014-06-18 ゲンマブ エー／エス 組換え一価抗体およびその作製方法
US20090082221A1 (en) 2006-05-04 2009-03-26 Kevin Caili Wang Cross-Species and Multi-Species Display Systems
CA2694364C (en) 2007-08-21 2016-11-22 Morphosys Ag Improved methods for the formation of disulphide bonds
EP2065386A1 (de) 2007-10-18 2009-06-03 Boehringer Ingelheim Pharma GmbH & Co. KG CGRP Antagonisten
JP5290306B2 (ja) 2007-10-19 2013-09-18 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 協調ノードの迅速な選択
US8637435B2 (en) 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
US20100317546A1 (en) 2008-01-11 2010-12-16 Morphosys Ag Display Vectors and Methods and Uses Thereof

Also Published As

Publication number Publication date
JP2015037419A (ja) 2015-02-26
AU2008290539A1 (en) 2009-02-26
EP2190987B1 (en) 2012-11-14
ZA201000975B (en) 2010-11-24
US20100190234A1 (en) 2010-07-29
JP6181626B2 (ja) 2017-08-16
CN101772574B (zh) 2016-01-06
IL203093A0 (en) 2011-08-01
US9062097B2 (en) 2015-06-23
DK2190987T3 (da) 2013-02-18
CN101772574A (zh) 2010-07-07
EP2190987A1 (en) 2010-06-02
ES2403904T3 (es) 2013-05-22
CA2694364A1 (en) 2009-02-26
KR20100089816A (ko) 2010-08-12
RU2010102859A (ru) 2011-09-27
AU2008290539B2 (en) 2013-07-25
WO2009024593A1 (en) 2009-02-26
CA2694364C (en) 2016-11-22
JP2011504722A (ja) 2011-02-17

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