MX2010000232A - Metodos mejorados para la formacion de enlaces disulfuro. - Google Patents

Metodos mejorados para la formacion de enlaces disulfuro.

Info

Publication number
MX2010000232A
MX2010000232A MX2010000232A MX2010000232A MX2010000232A MX 2010000232 A MX2010000232 A MX 2010000232A MX 2010000232 A MX2010000232 A MX 2010000232A MX 2010000232 A MX2010000232 A MX 2010000232A MX 2010000232 A MX2010000232 A MX 2010000232A
Authority
MX
Mexico
Prior art keywords
formation
disulphide bonds
improved methods
methods
peptides
Prior art date
Application number
MX2010000232A
Other languages
English (en)
Inventor
Josef Prassler
Yvonne Stark
Original Assignee
Morphosys Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morphosys Ag filed Critical Morphosys Ag
Publication of MX2010000232A publication Critical patent/MX2010000232A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a métodos para la formación de enlaces disulfuro intermoleculares, que incluyen (poli)péptidos/proteínas, ácidos nucleicos, vectores, células hospederas y bacteriófagos usados en estos métodos. Además, la invención se refiere al uso de este método para el despliegue mejorado de (poli) péptidos/proteínas en la superficie de las partículas del bacteriófago.
MX2010000232A 2007-08-21 2008-08-21 Metodos mejorados para la formacion de enlaces disulfuro. MX2010000232A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95702707P 2007-08-21 2007-08-21
US98903507P 2007-11-19 2007-11-19
PCT/EP2008/060931 WO2009024593A1 (en) 2007-08-21 2008-08-21 Improved methods for the formation of disulphide bonds

Publications (1)

Publication Number Publication Date
MX2010000232A true MX2010000232A (es) 2010-06-02

Family

ID=39929757

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010000232A MX2010000232A (es) 2007-08-21 2008-08-21 Metodos mejorados para la formacion de enlaces disulfuro.

Country Status (14)

Country Link
US (1) US9062097B2 (es)
EP (1) EP2190987B1 (es)
JP (2) JP2011504722A (es)
KR (1) KR20100089816A (es)
CN (1) CN101772574B (es)
AU (1) AU2008290539B2 (es)
CA (1) CA2694364C (es)
DK (1) DK2190987T3 (es)
ES (1) ES2403904T3 (es)
IL (1) IL203093A0 (es)
MX (1) MX2010000232A (es)
RU (1) RU2010102859A (es)
WO (1) WO2009024593A1 (es)
ZA (1) ZA201000975B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2403904T3 (es) 2007-08-21 2013-05-22 Morphosys Ag Métodos para la formación de enlaces disulfuro
MX2011012696A (es) 2009-05-29 2012-03-29 Morphosys Ag Una coleccion y metodos para su uso.
SG189950A1 (en) 2010-11-19 2013-06-28 Morphosys Ag A collection and methods for its use
SG194472A1 (en) * 2011-01-28 2013-12-30 Ca Nat Research Council Engineering of immunoglobulin domains
CN111954683A (zh) 2018-04-05 2020-11-17 生物辐射Abd瑟罗泰克有限公司 感兴趣的蛋白质的展示系统

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US413276A (en) * 1889-10-22 Central-station calling apparatus
ATE114723T1 (de) 1987-03-02 1994-12-15 Enzon Lab Inc Organismus als träger für ''single chain antibody domain (scad)''.
EP1892296A1 (en) 1988-09-02 2008-02-27 Dyax Corporation Generation and selection of recombinant varied binding proteins
US5545727A (en) * 1989-05-10 1996-08-13 Somatogen, Inc. DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin
US5747334A (en) 1990-02-15 1998-05-05 The University Of North Carolina At Chapel Hill Random peptide library
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5723286A (en) 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US5871974A (en) 1990-09-28 1999-02-16 Ixsys Inc. Surface expression libraries of heteromeric receptors
US5770434A (en) 1990-09-28 1998-06-23 Ixsys Incorporated Soluble peptides having constrained, secondary conformation in solution and method of making same
CA2405246A1 (en) 1990-12-03 1992-06-11 Genentech, Inc. Enrichment method for variant proteins with alterred binding properties
GB9114003D0 (en) 1991-06-28 1991-08-14 Mastico Robert A Chimaeric protein
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US6025165A (en) 1991-11-25 2000-02-15 Enzon, Inc. Methods for producing multivalent antigen-binding proteins
GB9213601D0 (en) 1992-06-26 1992-08-12 Mastico Robert A Protein based delivery system
CA2115811A1 (en) 1993-02-17 1994-08-18 Claus Krebber A method for in vivo selection of ligand-binding proteins
GB9313509D0 (en) 1993-06-30 1993-08-11 Medical Res Council Chemisynthetic libraries
US6309642B1 (en) 1997-04-28 2001-10-30 The Research And Development Institute, Inc. Peptides which mimic candida carbohydrate epitopes and their use in a vaccine
US5945311A (en) 1994-06-03 1999-08-31 GSF--Forschungszentrumfur Umweltund Gesundheit Method for producing heterologous bi-specific antibodies
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US6341256B1 (en) 1995-03-31 2002-01-22 Curagen Corporation Consensus configurational bias Monte Carlo method and system for pharmacophore structure determination
US6017754A (en) 1995-08-24 2000-01-25 Invitrogen Corporation System for isolating and identifying eukaryotic cells transfected with genes and vectors, host cells and methods thereof
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6420140B1 (en) 1996-10-11 2002-07-16 Abgenix, Inc. Production of multimeric protein by cell fusion method
US20020062010A1 (en) 1997-05-02 2002-05-23 Genentech, Inc. Method for making multispecific antibodies having heteromultimeric and common components
CA2297070A1 (en) 1997-08-01 1999-02-11 Morphosys Ag Novel method and phage for the identification of nucleic acid sequences encoding members of a multimeric (poly)peptide complex
US6046166A (en) * 1997-09-29 2000-04-04 Jean-Louis Dasseux Apolipoprotein A-I agonists and their use to treat dyslipidemic disorders
PT1078051E (pt) 1998-05-13 2008-03-17 Domantis Ltd Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas
US6962702B2 (en) 1998-06-22 2005-11-08 Immunomedics Inc. Production and use of novel peptide-based agents for use with bi-specific antibodies
US7052872B1 (en) 1999-06-22 2006-05-30 Immunomedics, Inc. Bi-specific antibodies for pre-targeting diagnosis and therapy
DE60041119D1 (de) * 1999-07-20 2009-01-29 Morphosys Ag Verfahren zur präsentation von (poly)peptiden/proteinen auf bakteriophagenpartikeln via disulfidbindungen
DK1355919T3 (da) 2000-12-12 2011-03-14 Medimmune Llc Molekyler med længere halveringstider, sammensætninger og anvendelser deraf
US6833441B2 (en) 2001-08-01 2004-12-21 Abmaxis, Inc. Compositions and methods for generating chimeric heteromultimers
EP2093286B1 (en) 2001-10-01 2013-02-27 Dyax Corporation Multi-chain eukaryotic display vectors and uses thereof
DE50115587D1 (de) 2001-10-01 2010-09-16 Deutsches Krebsforsch Verfahren zur Herstellung von Protein-Bibliotheken und zur Selektion von Proteinen daraus
US7175983B2 (en) * 2001-11-02 2007-02-13 Abmaxis, Inc. Adapter-directed display systems
AU2003253193B2 (en) 2002-07-30 2009-07-02 Morphosys Ag Novel tricistronic vectors and uses therefor
WO2005021710A2 (en) 2003-06-02 2005-03-10 University Of Miami Chimeric molecules and methods of use
AU2004309373A1 (en) 2003-12-22 2005-07-14 Amgen Inc. Methods for identifying functional antibodies
EP1725112A4 (en) 2004-02-05 2007-10-24 Massachusetts Inst Technology CELL DISPLAY LIBRARIES
WO2005092925A2 (en) 2004-03-24 2005-10-06 Xencor, Inc. Immunoglobulin variants outside the fc region
JP2008511337A (ja) 2004-09-02 2008-04-17 ジェネンテック・インコーポレーテッド ヘテロ多量体分子
TW200732350A (en) 2005-10-21 2007-09-01 Amgen Inc Methods for generating monovalent IgG
KR20080090406A (ko) 2005-11-28 2008-10-08 젠맵 에이/에스 재조합 １가 항체 및 그의 제조 방법
JP2009535063A (ja) 2006-05-04 2009-10-01 アブマクシス・インコーポレイテツド 異種間及び複数種提示系
ES2403904T3 (es) 2007-08-21 2013-05-22 Morphosys Ag Métodos para la formación de enlaces disulfuro
EP2065386A1 (de) 2007-10-18 2009-06-03 Boehringer Ingelheim Pharma GmbH & Co. KG CGRP Antagonisten
EP2213134B1 (en) 2007-10-19 2011-09-14 Koninklijke Philips Electronics N.V. Fast selection of cooperative nodes
US8637435B2 (en) 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
US20100317546A1 (en) 2008-01-11 2010-12-16 Morphosys Ag Display Vectors and Methods and Uses Thereof

Also Published As

Publication number Publication date
US9062097B2 (en) 2015-06-23
ZA201000975B (en) 2010-11-24
CN101772574B (zh) 2016-01-06
AU2008290539B2 (en) 2013-07-25
DK2190987T3 (da) 2013-02-18
JP2011504722A (ja) 2011-02-17
RU2010102859A (ru) 2011-09-27
AU2008290539A1 (en) 2009-02-26
CN101772574A (zh) 2010-07-07
EP2190987A1 (en) 2010-06-02
US20100190234A1 (en) 2010-07-29
EP2190987B1 (en) 2012-11-14
WO2009024593A1 (en) 2009-02-26
ES2403904T3 (es) 2013-05-22
IL203093A0 (en) 2011-08-01
KR20100089816A (ko) 2010-08-12
CA2694364C (en) 2016-11-22
JP2015037419A (ja) 2015-02-26
CA2694364A1 (en) 2009-02-26
JP6181626B2 (ja) 2017-08-16

Similar Documents

Publication Publication Date Title
WO2008140812A3 (en) Compositions and methods comprising klk3, psca, or folh1 antigen
IL250946B (en) Nanobodies against tumor necrosis factor-alpha (tnf-alpha)
WO2007001264A3 (en) Method of making recombinant human antibodies for use in biosensor technology
WO2010129304A3 (en) Method for making heteromultimeric molecules
EP2037737A4 (en) PROTEINS, NUCLEIC ACIDS FOR THEIR CODING AND CORRESPONDING USES.
JP2009225798A5 (es)
WO2010051274A3 (en) Fibronectin type iii domain based scaffold compositions, methods and uses
WO2010120514A3 (en) Antigen-binding proteins comprising recombinant protein scaffolds
MX2013003681A (es) Ácidos nucleicos manipulados y métodos de uso de los mismos.
EP2803675A3 (en) Ferroportin antibodies and methods of use
NZ596735A (en) Recombinant production of peptides
MX2021000030A (es) Metodos y composiciones que utilizan polipeptidos variantes klotho.
MX2010000232A (es) Metodos mejorados para la formacion de enlaces disulfuro.
EP4269562A3 (en) Antigen binding molecules and methods of use thereof
WO2015006728A3 (en) Lysine-specific chemoenzymatic protein modifications using microbial transglutaminase
EA201691011A1 (ru) Последовательность модифицированного эндолизина el188
EA201691004A1 (ru) Последовательность модифицированного эндолизина kz144
WO2007045019A3 (en) Polyoleosins
EP3415158A3 (en) Repeat variable diresidues for targeting nucleotides
EP2099467A4 (en) ATAP PEPTIDES, NUCLEIC ACIDS ENCODING THE SAME AND METHODS OF USING SAME
WO2014152247A8 (en) Improved tnf binding proteins
WO2007113336A3 (de) Funktionale expression von triacylglycerol-lipasen
WO2007089923A3 (en) Modifications of cst-ii for increased protein expression
MX2015009867A (es) Mutantes del factor x.
WO2009135953A3 (en) Amino acid sequences directed against integrins and uses thereof