HK1173188A1 - 噬菌體φ 聚合酶嵌合體 - Google Patents

噬菌體φ 聚合酶嵌合體

Info

Publication number
HK1173188A1
HK1173188A1 HK13100537.5A HK13100537A HK1173188A1 HK 1173188 A1 HK1173188 A1 HK 1173188A1 HK 13100537 A HK13100537 A HK 13100537A HK 1173188 A1 HK1173188 A1 HK 1173188A1
Authority
HK
Hong Kong
Prior art keywords
dna
phage
polymerase chimera
dna polymerase
chimera
Prior art date
Application number
HK13100537.5A
Other languages
English (en)
Inventor
Falgueras Margarita Salas
Vega Jos Miguel De
Bolos Jos M Lazaro
Davila Luis Blanco
Caballero Mario Mencia
Original Assignee
Consejo Superior De Investig Cientficas (Cs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior De Investig Cientficas (Cs filed Critical Consejo Superior De Investig Cientficas (Cs
Publication of HK1173188A1 publication Critical patent/HK1173188A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y599/00—Other isomerases (5.99)
    • C12Y599/01—Other isomerases (5.99.1)
    • C12Y599/01002—DNA topoisomerase (5.99.1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
HK13100537.5A 2009-07-02 2013-01-11 噬菌體φ 聚合酶嵌合體 HK1173188A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200930413A ES2359058B1 (es) 2009-07-02 2009-07-02 Quimera de adn polimerasa del fago ph1 29.
PCT/ES2010/070454 WO2011000997A1 (es) 2009-07-02 2010-07-01 QUIMERA DE ADN POLIMERASA DEL FAGO φ 29

Publications (1)

Publication Number Publication Date
HK1173188A1 true HK1173188A1 (zh) 2013-05-10

Family

ID=43410525

Family Applications (1)

Application Number Title Priority Date Filing Date
HK13100537.5A HK1173188A1 (zh) 2009-07-02 2013-01-11 噬菌體φ 聚合酶嵌合體

Country Status (24)

Country Link
US (2) US8404808B2 (zh)
EP (1) EP2450436B1 (zh)
JP (1) JP5778671B2 (zh)
KR (1) KR101751554B1 (zh)
CN (1) CN102648275B (zh)
AU (1) AU2010267954B2 (zh)
BR (1) BR112012000026A2 (zh)
CA (1) CA2766725C (zh)
CL (1) CL2011003312A1 (zh)
CO (1) CO6480970A2 (zh)
CY (1) CY1114747T1 (zh)
DK (1) DK2450436T3 (zh)
ES (2) ES2359058B1 (zh)
HK (1) HK1173188A1 (zh)
HR (1) HRP20131163T1 (zh)
IL (1) IL217324A0 (zh)
MX (1) MX2012000133A (zh)
NZ (1) NZ597884A (zh)
PL (1) PL2450436T3 (zh)
PT (1) PT2450436E (zh)
SG (1) SG177410A1 (zh)
SI (1) SI2450436T1 (zh)
SM (1) SMT201300153B (zh)
WO (1) WO2011000997A1 (zh)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2681332A1 (en) * 2011-02-28 2014-01-08 Transgenomic, Inc. Kit and method for sequencing a target dna in a mixed population
EP2971080B1 (en) 2013-03-15 2018-01-03 Expedeon, S.L. Methods for amplification and sequencing using thermostable tthprimpol
JP6594955B2 (ja) * 2014-08-27 2019-10-23 ニユー・イングランド・バイオレイブス・インコーポレイテツド シントン形成
CN105483115A (zh) * 2014-09-19 2016-04-13 深圳华大基因科技有限公司 一种用于单细胞全基因组扩增的试剂盒及其应用
US11312944B2 (en) * 2016-12-19 2022-04-26 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
EP3976771A1 (en) 2019-06-28 2022-04-06 Quantum-si Incorporated Polymerizing enzymes for sequencing reactions
CN110747191B (zh) * 2019-10-17 2021-08-17 深圳清华大学研究院 多肽、嵌合聚合酶及其应用
WO2021152147A1 (en) 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Closed linear dna with modified nucleotides
CA3164387A1 (en) 2020-01-31 2021-08-05 Julen Oyarzabal Santamarina Process for the production of closed linear dna
EP4299731A1 (en) 2022-06-29 2024-01-03 ASOCIACIÓN CENTRO DE INVESTIGACIÓN COOPERATIVA EN NANOCIENCIAS "CIC nanoGUNE" Synthetic primase-polymerase and uses thereof
WO2024138762A1 (zh) * 2022-12-30 2024-07-04 深圳华大智造科技股份有限公司 重组蛋白及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US20060234227A1 (en) * 2002-05-14 2006-10-19 Alexei Slesarev Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes
CA2519309A1 (en) * 2003-03-25 2004-10-14 Stratagene California Dna polymerase fusions and uses thereof
JP5241493B2 (ja) * 2005-07-15 2013-07-17 アジレント・テクノロジーズ・インク Ｄｎａ結合タンパク質−ポリメラーゼのキメラ

Also Published As

Publication number Publication date
JP2012531220A (ja) 2012-12-10
CN102648275B (zh) 2015-11-25
US20120190014A1 (en) 2012-07-26
AU2010267954A1 (en) 2012-02-16
IL217324A0 (en) 2012-02-29
ES2359058A1 (es) 2011-05-18
US20130236886A1 (en) 2013-09-12
EP2450436B1 (en) 2013-10-02
DK2450436T3 (da) 2014-01-13
CA2766725A1 (en) 2011-01-06
WO2011000997A1 (es) 2011-01-06
CN102648275A (zh) 2012-08-22
CY1114747T1 (el) 2016-12-14
CO6480970A2 (es) 2012-07-16
CA2766725C (en) 2016-10-11
NZ597884A (en) 2013-11-29
CL2011003312A1 (es) 2012-06-01
JP5778671B2 (ja) 2015-09-16
KR20120052954A (ko) 2012-05-24
ES2359058B1 (es) 2012-03-27
PL2450436T3 (pl) 2014-03-31
ES2438786T3 (es) 2014-01-20
US8404808B2 (en) 2013-03-26
SG177410A1 (en) 2012-02-28
HRP20131163T1 (hr) 2014-02-14
AU2010267954B2 (en) 2014-12-11
PT2450436E (pt) 2013-12-27
SI2450436T1 (sl) 2014-02-28
EP2450436A4 (en) 2012-11-28
SMT201300153B (it) 2014-03-07
KR101751554B1 (ko) 2017-06-28
BR112012000026A2 (pt) 2015-09-08
EP2450436A1 (en) 2012-05-09
MX2012000133A (es) 2012-04-30

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Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20200706