CO6480970A2 - Quimera de adn polimerasa del fago o 29 - Google Patents

Quimera de adn polimerasa del fago o 29

Info

Publication number
CO6480970A2
CO6480970A2 CO11180682A CO11180682A CO6480970A2 CO 6480970 A2 CO6480970 A2 CO 6480970A2 CO 11180682 A CO11180682 A CO 11180682A CO 11180682 A CO11180682 A CO 11180682A CO 6480970 A2 CO6480970 A2 CO 6480970A2
Authority
CO
Colombia
Prior art keywords
dna polymerase
phago
chimera
carrying
biotechnology
Prior art date
Application number
CO11180682A
Other languages
English (en)
Inventor
Falgueras Margarita Salas
Vega Jose Miguel De
Bolos Jose Ma Lazaro
Davila Luis Blanco
Caballero Mario Mencia
Original Assignee
Consejo Superior Investigaciones Cientificas Csic
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior Investigaciones Cientificas Csic filed Critical Consejo Superior Investigaciones Cientificas Csic
Publication of CO6480970A2 publication Critical patent/CO6480970A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y599/00—Other isomerases (5.99)
    • C12Y599/01—Other isomerases (5.99.1)
    • C12Y599/01002—DNA topoisomerase (5.99.1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invención se encuadra dentro del campo de la biotecnología. Específicamente se refiere a un método para llevar a cabo la replicación, la amplificación o la secuenciación de un ácido ácido cori una ADN polimerasa del tipo a29 y una kit para llevar a cabo dicho método.
CO11180682A 2009-07-02 2011-12-29 Quimera de adn polimerasa del fago o 29 CO6480970A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200930413A ES2359058B1 (es) 2009-07-02 2009-07-02 Quimera de adn polimerasa del fago ph1 29.

Publications (1)

Publication Number Publication Date
CO6480970A2 true CO6480970A2 (es) 2012-07-16

Family

ID=43410525

Family Applications (1)

Application Number Title Priority Date Filing Date
CO11180682A CO6480970A2 (es) 2009-07-02 2011-12-29 Quimera de adn polimerasa del fago o 29

Country Status (24)

Country Link
US (2) US8404808B2 (es)
EP (1) EP2450436B1 (es)
JP (1) JP5778671B2 (es)
KR (1) KR101751554B1 (es)
CN (1) CN102648275B (es)
AU (1) AU2010267954B2 (es)
BR (1) BR112012000026A2 (es)
CA (1) CA2766725C (es)
CL (1) CL2011003312A1 (es)
CO (1) CO6480970A2 (es)
CY (1) CY1114747T1 (es)
DK (1) DK2450436T3 (es)
ES (2) ES2359058B1 (es)
HK (1) HK1173188A1 (es)
HR (1) HRP20131163T1 (es)
IL (1) IL217324A0 (es)
MX (1) MX2012000133A (es)
NZ (1) NZ597884A (es)
PL (1) PL2450436T3 (es)
PT (1) PT2450436E (es)
SG (1) SG177410A1 (es)
SI (1) SI2450436T1 (es)
SM (1) SMT201300153B (es)
WO (1) WO2011000997A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103517993A (zh) * 2011-02-28 2014-01-15 环球基因有限公司 用于对混合群体中的靶dna进行测序的试剂盒和方法
CN105283558B (zh) 2013-03-15 2019-05-10 西格尼斯生物技术有限责任公司 使用热稳定的TthPrimPol扩增和测序的方法
CN107075511B9 (zh) * 2014-08-27 2021-10-26 新英格兰生物实验室公司 合成子的形成
CN105483115A (zh) * 2014-09-19 2016-04-13 深圳华大基因科技有限公司 一种用于单细胞全基因组扩增的试剂盒及其应用
JP2020501573A (ja) * 2016-12-19 2020-01-23 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ シークエンシング反応用の重合酵素
EP3976771A1 (en) 2019-06-28 2022-04-06 Quantum-si Incorporated Polymerizing enzymes for sequencing reactions
CN110747191B (zh) * 2019-10-17 2021-08-17 深圳清华大学研究院 多肽、嵌合聚合酶及其应用
WO2021152147A1 (en) 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Closed linear dna with modified nucleotides
WO2021152146A1 (en) 2020-01-31 2021-08-05 Tyris Therapeutics, S.L. Process for the production of closed linear dna
EP4299731A1 (en) 2022-06-29 2024-01-03 ASOCIACIÓN CENTRO DE INVESTIGACIÓN COOPERATIVA EN NANOCIENCIAS "CIC nanoGUNE" Synthetic primase-polymerase and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
WO2004013279A2 (en) * 2002-05-14 2004-02-12 Fidelity Systems, Inc. Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes
WO2004087868A2 (en) * 2003-03-25 2004-10-14 Stratagene Dna polymerase fusions and uses thereof
WO2007011891A2 (en) 2005-07-15 2007-01-25 Strategene California Dna binding protein-polymerase chimeras

Also Published As

Publication number Publication date
HRP20131163T1 (hr) 2014-02-14
KR20120052954A (ko) 2012-05-24
JP2012531220A (ja) 2012-12-10
AU2010267954A1 (en) 2012-02-16
ES2438786T3 (es) 2014-01-20
DK2450436T3 (da) 2014-01-13
US8404808B2 (en) 2013-03-26
US20130236886A1 (en) 2013-09-12
HK1173188A1 (zh) 2013-05-10
NZ597884A (en) 2013-11-29
US20120190014A1 (en) 2012-07-26
SI2450436T1 (sl) 2014-02-28
CY1114747T1 (el) 2016-12-14
CA2766725C (en) 2016-10-11
CN102648275A (zh) 2012-08-22
PL2450436T3 (pl) 2014-03-31
AU2010267954B2 (en) 2014-12-11
CA2766725A1 (en) 2011-01-06
IL217324A0 (en) 2012-02-29
MX2012000133A (es) 2012-04-30
EP2450436A4 (en) 2012-11-28
ES2359058A1 (es) 2011-05-18
BR112012000026A2 (pt) 2015-09-08
PT2450436E (pt) 2013-12-27
JP5778671B2 (ja) 2015-09-16
SMT201300153B (it) 2014-03-07
KR101751554B1 (ko) 2017-06-28
SG177410A1 (en) 2012-02-28
CL2011003312A1 (es) 2012-06-01
WO2011000997A1 (es) 2011-01-06
ES2359058B1 (es) 2012-03-27
EP2450436A1 (en) 2012-05-09
EP2450436B1 (en) 2013-10-02
CN102648275B (zh) 2015-11-25

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