CY1106432T1 - Μεθοδος και συσκευη για ενισχυση αλληλουχιων νουκλεϊνικου οξεως με χρηση μεταφορας θepμοτητος δια ρευματων - Google Patents

Μεθοδος και συσκευη για ενισχυση αλληλουχιων νουκλεϊνικου οξεως με χρηση μεταφορας θepμοτητος δια ρευματων

Info

Publication number
CY1106432T1
CY1106432T1 CY20071100459T CY071100459T CY1106432T1 CY 1106432 T1 CY1106432 T1 CY 1106432T1 CY 20071100459 T CY20071100459 T CY 20071100459T CY 071100459 T CY071100459 T CY 071100459T CY 1106432 T1 CY1106432 T1 CY 1106432T1
Authority
CY
Cyprus
Prior art keywords
nucleic acid
currents
heat transfer
acid sequences
amplifying nucleic
Prior art date
Application number
CY20071100459T
Other languages
English (en)
Inventor
Hyun Jin Hwang
Jeong Hee Kim
Kyunghoon Jeong
της Κορέας Δημοκρατία
Original Assignee
Ahram Biosystems Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ahram Biosystems Inc. filed Critical Ahram Biosystems Inc.
Publication of CY1106432T1 publication Critical patent/CY1106432T1/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1883—Means for temperature control using thermal insulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B01L2400/0445—Natural or forced convection

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

Η παρούσα εφεύρεση παρέχει μέθοδο ενίσχυσης αλληλουχίας νουκλεϊνικού οξέος και συσκευές γι αυτήν που είναι απλές στο σχεδιασμό και εύκολες να γίνουν σε μικρογραφία και να ολοκληρωθούν σε πολύπλοκες συσκευές με ικανότητα χρήσης DNA πολυμερασών που δεν είναι θερμοσταθερές. Στην παρούσα εφεύρεση, συνδυάζεται μία πλειονότητα πηγών θερμότητος για να παρέχεται θερμότητα σε ειδικές περιοχές ή να απομακρύνεται θερμότητα από ειδικές περιοχές του δείγματος, έτσι ώστε διατηρείται μία ειδική στο χώρο κατανομή θερμοκρασίας εντός του δείγματος με τοποθέτηση μιας περιοχής σχετικά υψηλής θερμοκρασίας χαμηλότερα στο ύψος απ' ότι μία περιοχή σχετικώς χαμηλής θερμοκρασίας.
CY20071100459T 2001-09-15 2007-04-02 Μεθοδος και συσκευη για ενισχυση αλληλουχιων νουκλεϊνικου οξεως με χρηση μεταφορας θepμοτητος δια ρευματων CY1106432T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20010057040 2001-09-15
KR20010066943 2001-10-30
PCT/KR2002/001728 WO2003025226A1 (en) 2001-09-15 2002-09-14 Method and apparatus for amplification of nucleic acid sequences by using thermal convection

Publications (1)

Publication Number Publication Date
CY1106432T1 true CY1106432T1 (el) 2011-10-12

Family

ID=26639348

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20071100459T CY1106432T1 (el) 2001-09-15 2007-04-02 Μεθοδος και συσκευη για ενισχυση αλληλουχιων νουκλεϊνικου οξεως με χρηση μεταφορας θepμοτητος δια ρευματων

Country Status (13)

Country Link
US (4) US7628961B2 (el)
EP (1) EP1434880B1 (el)
JP (2) JP4357962B2 (el)
KR (1) KR100488281B1 (el)
CN (2) CN101003825B (el)
AT (1) ATE350491T1 (el)
CY (1) CY1106432T1 (el)
DE (1) DE60217375T2 (el)
DK (1) DK1434880T3 (el)
ES (1) ES2280567T3 (el)
HK (1) HK1109425A1 (el)
PT (1) PT1434880E (el)
WO (1) WO2003025226A1 (el)

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US20040152122A1 (en) 2004-08-05
US7628961B2 (en) 2009-12-08
DK1434880T3 (da) 2007-05-14
JP2005516588A (ja) 2005-06-09
ES2280567T3 (es) 2007-09-16
ATE350491T1 (de) 2007-01-15
US20140170707A1 (en) 2014-06-19
JP4357962B2 (ja) 2009-11-04
DE60217375T2 (de) 2007-10-18
KR20030024592A (ko) 2003-03-26
US8053215B2 (en) 2011-11-08
CN1571849A (zh) 2005-01-26
JP4477089B2 (ja) 2010-06-09
CN101003825B (zh) 2012-08-22
US20100285536A1 (en) 2010-11-11
JP2009100761A (ja) 2009-05-14
CN101003825A (zh) 2007-07-25
WO2003025226A1 (en) 2003-03-27
PT1434880E (pt) 2007-04-30
US20120021463A1 (en) 2012-01-26
EP1434880A1 (en) 2004-07-07
KR100488281B1 (ko) 2005-05-10
EP1434880B1 (en) 2007-01-03
US9765376B2 (en) 2017-09-19
HK1109425A1 (en) 2008-06-06
EP1434880A4 (en) 2005-08-10
CN1304597C (zh) 2007-03-14
DE60217375D1 (de) 2007-02-15

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