CY1105772T1 - Συνθεσεις και μεθοδοι για βελτιωμενη κυτταρικη καλλιεργεια - Google Patents

Συνθεσεις και μεθοδοι για βελτιωμενη κυτταρικη καλλιεργεια

Info

Publication number
CY1105772T1
CY1105772T1 CY20061101630T CY061101630T CY1105772T1 CY 1105772 T1 CY1105772 T1 CY 1105772T1 CY 20061101630 T CY20061101630 T CY 20061101630T CY 061101630 T CY061101630 T CY 061101630T CY 1105772 T1 CY1105772 T1 CY 1105772T1
Authority
CY
Cyprus
Prior art keywords
cell culture
methods
compositions
improved cell
igf
Prior art date
Application number
CY20061101630T
Other languages
English (en)
Inventor
Arvia E. Morris
Pranhitha Reddy
Original Assignee
Immunex Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunex Corporation filed Critical Immunex Corporation
Publication of CY1105772T1 publication Critical patent/CY1105772T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Η εφεύρεση παρέχει βελτιωμένες μεθόδους παραγωγής ανασυνδυασμένης πρωτεΐνης σε κυτταρική καλλιέργεια. Ειδικότερα, η εφεύρεση σχετίζεται με την διαμόρφωση του μονοπατιού σηματοδότησης IGF-1 σε κύτταρα έτσι ώστε να βελτιωθούν τα χαρακτηριστικά παραγωγής.
CY20061101630T 1999-08-25 2006-11-13 Συνθεσεις και μεθοδοι για βελτιωμενη κυτταρικη καλλιεργεια CY1105772T1 (el)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US15064599P 1999-08-25 1999-08-25
US16894899P 1999-12-03 1999-12-03
US17194999P 1999-12-23 1999-12-23
PCT/US2000/023483 WO2001014529A1 (en) 1999-08-25 2000-08-25 Compositions and methods for improved cell culture

Publications (1)

Publication Number Publication Date
CY1105772T1 true CY1105772T1 (el) 2011-02-02

Family

ID=27387011

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20061101630T CY1105772T1 (el) 1999-08-25 2006-11-13 Συνθεσεις και μεθοδοι για βελτιωμενη κυτταρικη καλλιεργεια

Country Status (14)

Country Link
US (3) US6413744B1 (el)
EP (1) EP1210411B1 (el)
JP (1) JP2003507058A (el)
AT (1) ATE342962T1 (el)
AU (1) AU784506B2 (el)
CA (1) CA2383073A1 (el)
CY (1) CY1105772T1 (el)
DE (1) DE60031419T2 (el)
DK (1) DK1210411T3 (el)
ES (1) ES2272318T3 (el)
IL (2) IL148060A0 (el)
NZ (2) NZ529329A (el)
PT (1) PT1210411E (el)
WO (1) WO2001014529A1 (el)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US7115231B1 (en) 1998-06-09 2006-10-03 Symyx Technologies, Inc. Parallel reactor with knife-edge seal
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
IL148060A0 (en) * 1999-08-25 2002-09-12 Immunex Corp Compositions and methods for improved cell culture
IL136232A0 (en) * 2000-05-18 2001-05-20 Bar Ilan University Res Author Measurements of enzymatic activity in a single, individual cell in population
US6439036B1 (en) 2000-06-13 2002-08-27 Symyx Technologics, Inc. Method for evaluating a test fluid
US6455007B1 (en) 2000-06-13 2002-09-24 Symyx Technologies, Inc. Apparatus and method for testing compositions in contact with a porous medium
US7018589B1 (en) 2000-07-19 2006-03-28 Symyx Technologies, Inc. High pressure parallel reactor
US7091004B1 (en) 2001-06-08 2006-08-15 Immunex Corporation Regulation of translation for recombinant protein production
US20050271663A1 (en) * 2001-06-28 2005-12-08 Domantis Limited Compositions and methods for treating inflammatory disorders
US20060073141A1 (en) * 2001-06-28 2006-04-06 Domantis Limited Compositions and methods for treating inflammatory disorders
DE60236379D1 (de) * 2001-07-04 2010-06-24 Chromagenics Bv DNS-Sequenzen mit anti-repressor Aktivität
JP4192097B2 (ja) * 2001-10-25 2008-12-03 バル−イラン ユニバーシティ 相互作用型透明個別細胞バイオチッププロセッサー
US20030166147A1 (en) * 2001-12-14 2003-09-04 Lee Chichang Clonal myeloma cell lines useful for manufacturing proteins in chemically defined media
DK1520008T3 (da) 2002-07-09 2012-10-15 Baxter Int Medium frit for dyreprotein til dyrkning af celler
DE60312039T2 (de) * 2002-12-20 2007-11-08 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
IL154677A0 (en) 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
US8597597B2 (en) * 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
WO2004113492A1 (en) * 2003-06-26 2004-12-29 Molecular Cytomics Ltd. Improved materials for constructing cell-chips, cell-chip covers, cell-chip coats, processed cell-chips and uses thereof
US7888110B2 (en) 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
WO2005008626A1 (en) * 2003-07-11 2005-01-27 University Of North Carolina At Chapel Hill Methods and systems for controlling a computer using a video image and for combining the video image with a computer desktop
US20050064524A1 (en) * 2003-08-11 2005-03-24 Mordechai Deutsch Population of cells utilizable for substance detection and methods and devices using same
BRPI0506610A (pt) * 2004-02-03 2007-05-02 Chemagis Ltd montelucaste sódico amorfo, processo de preparação de montelucaste sódico amorfo, composição farmacêutica, método de tratamento utilizando a administração de montelucaste sódico amorfo, co-precipitado amorfo de montelucaste sódico e processo de preparação de co-precipitado amorfo de montelucaste sódico
US20080063251A1 (en) * 2004-07-07 2008-03-13 Mordechai Deutsch Method and Device for Identifying an Image of a Well in an Image of a Well-Bearing
US7403647B2 (en) * 2004-09-13 2008-07-22 Seng Enterprises Ltd. Method for identifying an image of a well in an image of a well-bearing component
WO2006021959A2 (en) * 2004-08-25 2006-03-02 Seng Enterprises Ltd. Method and device for isolating cells
CN101065479A (zh) * 2004-10-15 2007-10-31 蒙奈尔化学感觉中心 培养哺乳动物味细胞的方法
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060172382A1 (en) * 2004-11-08 2006-08-03 Chromagenics B.V. Selection of host cells expressing protein at high levels
PL1809750T3 (pl) * 2004-11-08 2012-08-31 Chromagenics Bv Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach
JP2008527980A (ja) * 2005-01-25 2008-07-31 アポロ ライフ サイエンシズ リミテッド 分子およびそのキメラ分子
ES2352344T3 (es) 2005-01-25 2011-02-17 Seng Enterprises Limited Dispositivo de microfluido para estudio de células.
EP1707634A1 (en) 2005-03-29 2006-10-04 Octapharma AG Method for isolation of recombinantly produced proteins
US20060232821A1 (en) * 2005-04-15 2006-10-19 Sridharan Ranganathan Parallel architecture for vector color error diffusion
WO2007002210A2 (en) * 2005-06-20 2007-01-04 Bresagen, Inc. Embryonic stem cell culture compositions and methods of use thereof
EP1739179A1 (en) 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
DE102005034043B4 (de) * 2005-07-18 2019-12-12 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Gemisch, enthaltend L-Carnitin und Trehalulose, sowie Produkt enthaltend das Gemisch
US20070141555A1 (en) * 2005-10-11 2007-06-21 Mordechai Deutsch Current damper for the study of cells
US8288120B2 (en) * 2005-11-03 2012-10-16 Seng Enterprises Ltd. Method for studying floating, living cells
EP3653699A1 (en) 2006-01-04 2020-05-20 Baxalta Incorporated Oligopeptide-free cell culture media
US20060223999A1 (en) * 2006-05-10 2006-10-05 Chemagis Ltd. Process for preparing montelukast and precursors thereof
US7655191B2 (en) 2007-05-14 2010-02-02 Symyx Solutions, Inc. Methods for chemical reactions in a parallel batch reactor
US7807109B2 (en) 2007-05-14 2010-10-05 Freeslate, Inc. Parallel batch reactor with pressure monitoring
MX2009012968A (es) * 2007-06-06 2010-04-01 Domantis Ltd Polipeptidos, dominios variables de anticuerpo y antagonistas.
RU2010102810A (ru) * 2007-06-29 2011-08-10 Ф.Хоффманн-Ля Рош Аг (Ch) Клетки сно
HUE039950T2 (hu) * 2007-10-12 2019-02-28 Hoffmann La Roche Fehérjeexpresszió több nukleinsavból
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
WO2009081409A2 (en) 2007-12-26 2009-07-02 Seng Enterprises Ltd. Device for the study of living cells
EP2257620A1 (de) * 2008-03-19 2010-12-08 Boehringer Ingelheim Pharma GmbH & Co. KG Methode zur steigerung der reklonierungseffizienz
CN102224239A (zh) * 2008-09-26 2011-10-19 先灵公司 高效价抗体生产
KR20140015152A (ko) * 2009-12-02 2014-02-06 악셀레론 파마 인코포레이티드 Ｆｃ 융합 단백질의 혈청 반감기를 증가시키는 조성물들 및 방법들
EP2767546B1 (en) 2011-02-07 2018-10-17 Cerenis Therapeutics Holding SA Lipoprotein complexes and manufacturing and uses therof
WO2012170938A1 (en) 2011-06-08 2012-12-13 Acceleron Pharma Inc. Compositions and methods for increasing serum half-life
CN104822826B (zh) 2012-10-15 2019-04-30 百时美施贵宝公司 用于蛋白产生的哺乳动物细胞培养过程
WO2015010088A1 (en) * 2013-07-19 2015-01-22 Technical University Of Denmark Methods for modeling chinese hamster ovary (cho) cell metabolism
US20180237534A1 (en) 2015-05-29 2018-08-23 Bristol-Myers Squibb Company Antibodies against ox40 and uses thereof
CN113493770A (zh) * 2020-03-18 2021-10-12 苏州大学 人源glut5稳定转染细胞株及其在筛选glut5抑制剂中的应用
WO2022140389A1 (en) 2020-12-22 2022-06-30 Amgen Inc. Cell culture method

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL87737A (en) 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
WO1991003554A1 (en) * 1989-09-01 1991-03-21 Genentech, Inc. Cells transfected with nucleic acid encoding gtp
US5605690A (en) * 1989-09-05 1997-02-25 Immunex Corporation Methods of lowering active TNF-α levels in mammals using tumor necrosis factor receptor
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
EP0496453A1 (en) 1991-01-23 1992-07-29 Merck & Co. Inc. Human glut4 gene promoter
US5262308A (en) * 1992-01-28 1993-11-16 Thomas Jefferson University Cell lines which constitutively express IGF-1 and IGF-1 R
WO1993020200A1 (en) 1992-04-02 1993-10-14 Imperial Cancer Research Technology Limited Modified cells and method of treatment
US5663314A (en) 1992-11-20 1997-09-02 The Board Of Regents Of The University Of Washington Human signal transduction MAPK kinase
EP0666312A1 (en) * 1994-02-08 1995-08-09 Wolfgang A. Renner Process for the improvement of mammalian cell growth
US5597719A (en) * 1994-07-14 1997-01-28 Onyx Pharmaceuticals, Inc. Interaction of RAF-1 and 14-3-3 proteins
AUPN442295A0 (en) * 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells
CA2298854A1 (en) 1997-09-26 1999-04-08 Onyx Pharmaceuticals, Inc. Compositions and methods for identifying pkb kinase inhibitors
IL148060A0 (en) * 1999-08-25 2002-09-12 Immunex Corp Compositions and methods for improved cell culture

Also Published As

Publication number Publication date
EP1210411A1 (en) 2002-06-05
JP2003507058A (ja) 2003-02-25
US20050170462A1 (en) 2005-08-04
US6413744B1 (en) 2002-07-02
AU7077800A (en) 2001-03-19
US6897040B2 (en) 2005-05-24
EP1210411B1 (en) 2006-10-18
DE60031419D1 (de) 2006-11-30
DE60031419T2 (de) 2007-09-27
ATE342962T1 (de) 2006-11-15
WO2001014529A1 (en) 2001-03-01
US20020160455A1 (en) 2002-10-31
US7166445B2 (en) 2007-01-23
IL148060A (en) 2009-07-20
NZ529329A (en) 2005-06-24
IL148060A0 (en) 2002-09-12
PT1210411E (pt) 2006-12-29
ES2272318T3 (es) 2007-05-01
NZ517215A (en) 2004-01-30
CA2383073A1 (en) 2001-03-01
AU784506B2 (en) 2006-04-13
WO2001014529A9 (en) 2002-09-06
DK1210411T3 (da) 2007-02-19

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