PL1809750T3 - Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach - Google Patents

Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach

Info

Publication number
PL1809750T3
PL1809750T3 PL05810935T PL05810935T PL1809750T3 PL 1809750 T3 PL1809750 T3 PL 1809750T3 PL 05810935 T PL05810935 T PL 05810935T PL 05810935 T PL05810935 T PL 05810935T PL 1809750 T3 PL1809750 T3 PL 1809750T3
Authority
PL
Poland
Prior art keywords
selection
host cells
cells expressing
high levels
expressing protein
Prior art date
Application number
PL05810935T
Other languages
English (en)
Inventor
Arie Pieter Otte
Blokland Henricus Johannes Maria Van
Theodorus Hendrikus Jacobus Kwaks
Richard George Antonius Bernardus Sewalt
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Publication of PL1809750T3 publication Critical patent/PL1809750T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
PL05810935T 2004-11-08 2005-11-07 Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach PL1809750T3 (pl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US62630104P 2004-11-08 2004-11-08
EP04105593 2004-11-08
US69661005P 2005-07-05 2005-07-05
PCT/EP2005/055794 WO2006048459A2 (en) 2004-11-08 2005-11-07 Selection of host cells expressing protein at high levels
EP05810935A EP1809750B1 (en) 2004-11-08 2005-11-07 Selection of host cells expressing protein at high levels

Publications (1)

Publication Number Publication Date
PL1809750T3 true PL1809750T3 (pl) 2012-08-31

Family

ID=36090897

Family Applications (1)

Application Number Title Priority Date Filing Date
PL05810935T PL1809750T3 (pl) 2004-11-08 2005-11-07 Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach

Country Status (14)

Country Link
US (1) US20060141577A1 (pl)
EP (1) EP1809750B1 (pl)
JP (1) JP5291341B2 (pl)
KR (1) KR101271884B1 (pl)
AU (1) AU2005300503B2 (pl)
BR (1) BRPI0517380A (pl)
CA (1) CA2581422C (pl)
EA (1) EA010863B1 (pl)
HK (1) HK1101264A1 (pl)
IL (1) IL183035A0 (pl)
MX (1) MX2007004595A (pl)
NO (1) NO344469B1 (pl)
PL (1) PL1809750T3 (pl)
WO (1) WO2006048459A2 (pl)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654442A (en) * 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
DK1573058T3 (da) * 2002-12-18 2012-01-16 Chromagenics Bv Fremgangsmåde til forbedring af proteinproduktion
EP1572994B1 (en) 2002-12-20 2007-02-21 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
NZ553700A (en) * 2004-11-08 2010-09-30 Chromagenics Bv Selection of host cells expressing protein at high levels using a selectable marker with a non-optimal start codon
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1910550A4 (en) 2005-07-21 2009-11-04 Abbott Lab MULTIGENIC EXPRESSION COMPRISING SORF CONSTRUCTS AND METHODS USING POLYPROTEINS, PRO-PROTEINS, AND PROTEOLYSIS
PT1987150E (pt) * 2006-02-21 2011-09-08 Chromagenics Bv Selecção de células hospedeiras expressando níveis elevados de proteína
EP1996705B1 (en) 2006-03-20 2011-08-31 ChromaGenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof
EP2064337B1 (en) * 2006-09-20 2012-07-18 Genzyme Corporation A facs-and reporter protein-based system for high throughput development of therapeutic proteins
US20080274137A1 (en) * 2007-05-02 2008-11-06 Jean Christophe Francis Audonnet DNA plasmids having improved expression and stability
AU2009218069B2 (en) * 2008-02-27 2014-05-01 Fuso Pharmaceutical Industries, Ltd. Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof
WO2010026443A1 (en) * 2008-09-08 2010-03-11 Cellectis Meganuclease variants cleaving a dna target sequence from a glutamine synthetase gene and uses thereof
KR101655492B1 (ko) * 2008-12-22 2016-09-07 국립대학법인 홋가이도 다이가쿠 동물세포를 사용해서 외래유전자 유래 단백질을 대량으로 생산하기 위한 발현 벡터, 및 그의 이용
US9284563B2 (en) 2009-06-15 2016-03-15 Cellagenics B.V. Stringent selectable markers
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
WO2011159157A1 (en) 2010-06-15 2011-12-22 Cellagenics B.V. Novel intergenic elements for enhancing gene expression
US9650630B2 (en) 2010-09-01 2017-05-16 Cellagenics B.V. Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression
ES2716577T3 (es) 2012-07-31 2019-06-13 Agex Therapeutics Inc Células HLA-G modificadas y métodos
MA40902B1 (fr) 2014-11-04 2018-12-31 Janssen Vaccines & Prevention Bv Vaccins hpv16 thérapeutiques
WO2017029360A1 (en) 2015-08-20 2017-02-23 Janssen Vaccines & Prevention B.V. Therapeutic hpv18 vaccines
WO2017062722A2 (en) 2015-10-09 2017-04-13 Genzyme Corporation Improved flare (flow cytometry attenuated reporter expression) technology for rapid bulk sorting
US10517944B2 (en) 2016-05-02 2019-12-31 Janssen Vaccines & Prevention B.V. Therapeutic HPV vaccine combinations
US20180119192A1 (en) 2016-10-07 2018-05-03 Genzyme Corporation Early post-transfection isolation of cells (epic) for biologics production
AU2019273200B2 (en) * 2018-05-24 2024-10-31 Fuso Pharmaceutical Industries,Ltd. Novel vector and use thereof
WO2020036181A1 (ja) * 2018-08-13 2020-02-20 Ｓｐｉｂｅｒ株式会社 細胞を単離又は同定する方法及び細胞集団
WO2020068631A1 (en) 2018-09-24 2020-04-02 Merck Sharp & Dohme Corp. Expression vectors for eukaryotic expression systems
IL302786A (en) 2020-11-19 2023-07-01 Genzyme Corp Flow cytometry Attenuated reporter expression (flaring) Multi-reporter system and methods of using it

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3700633A (en) * 1971-05-05 1972-10-24 Shell Oil Co Selectively hydrogenated block copolymers
US3821148A (en) * 1973-09-28 1974-06-28 Exxon Research Engineering Co Plasticized thermoplastic block copolymers
US4122134A (en) * 1974-02-13 1978-10-24 Sumitomo Chemical Company, Limited Method for producing transparent block copolymer resin
US3965830A (en) * 1974-12-05 1976-06-29 Usm Corporation Assembly for automatic bar tacking
US4267284A (en) * 1977-01-07 1981-05-12 Phillips Petroleum Company Tough, transparent articles from styrene polymers blended with certain block-random copolymers
US4965196A (en) 1983-01-19 1990-10-23 Genentech, Inc. Polycistronic expression vector construction
FR2569723B1 (fr) * 1984-08-31 1986-09-19 Centre Nat Rech Scient Application des antibiotiques de la famille des phleomycines a titre d'agent de selection dans le domaine du genie genetique
US4835200A (en) * 1986-12-19 1989-05-30 Shell Oil Company Color stable hot melt adhesive
US4882384A (en) * 1988-02-01 1989-11-21 Shell Oil Company Modified block copolymers
EP0484411A4 (en) * 1989-07-24 1992-06-17 Seragen, Inc. Prevention of internal initiation
US5256568A (en) * 1990-02-12 1993-10-26 Regeneron Phamaceuticals, Inc. Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers
ES2153358T3 (es) 1991-03-29 2001-03-01 Genentech Inc Procedimientos para la seleccion de celulas huespedes recombinantes que expresan altos niveles de una proteina deseada.
US6319707B1 (en) * 1991-08-12 2001-11-20 Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington Cap-independent multicistronic retroviral vectors
JP2686025B2 (ja) * 1992-08-25 1997-12-08 理化学研究所 ブラストサイジンｓデアミナーゼ遺伝子
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
DE69332859T2 (de) 1992-11-13 2003-12-18 Idec Pharmaceuticals Corp., San Diego Konsensus-kozak-sequenzen zur säugetier-exprimierung
AU6498294A (en) 1993-04-07 1994-10-24 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Dna sequence which acts as a chromatin insulator element to protect expressed genes from (cis)-acting regulatory sequences in mammalian cells
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5561053A (en) 1994-08-05 1996-10-01 Genentech, Inc. Method for selecting high-expressing host cells
US5627033A (en) 1995-03-28 1997-05-06 Research & Diagnostics Systems, Inc. Mammalian expression vectors
DE69633565T3 (de) 1995-06-15 2013-01-17 Crucell Holland B.V. Verpackungssysteme für humane, menschliche adenoviren, zur verwendung in die gentherapie
DE19523585A1 (de) * 1995-06-29 1997-01-02 Basf Ag Schlagzähe Formmasse aus Styrolpolymeren
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
DE19621688A1 (de) * 1996-05-30 1997-12-04 Basf Ag Transparente, nicht-blockende Folie
US6107477A (en) * 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences
WO1998039411A1 (en) 1997-03-04 1998-09-11 Baxter International Inc. Adenovirus e1-complementing cell lines
US5888809A (en) * 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
DE19832245A1 (de) 1998-07-17 2000-01-20 Heidelberger Druckmasch Ag Verfahren zum Bedienen von Geräten in der Reproduktionstechnik
US6689606B2 (en) 1998-07-21 2004-02-10 M.L. Laboratories Plc Polynucleotide
AU771111B2 (en) 1998-07-21 2004-03-11 Emd Millipore Corporation A polynucleotide comprising a ubiquitous chromatin opening element (UCOE)
EP1104490B8 (en) * 1998-08-14 2008-02-13 Chromagenics B.V. Method of detecting a dna sequence, a dna sequence, a method of making a dna construct and the use thereof
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6800457B2 (en) * 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2000023606A1 (en) * 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
PT1161548E (pt) 1999-04-15 2005-06-30 Crucell Holland Bv Producao de proteinas recombinantes numa celula humana utilizando sequencias codificando a proteina e1 de adenovirus
US6391981B1 (en) * 1999-04-23 2002-05-21 Kraton Polymers Us Llc Increased throughput in the manufacture of anionic polymers by reduction in polymer cement viscosity through the addition of metal alkyls
JP2003504016A (ja) 1999-06-30 2003-02-04 アメリカ合衆国 遺伝子発現の制御のための特異的エンハンサー遮断活性を有する絶縁体としてのｄｎａ結合タンパク質および配列
US6413744B1 (en) * 1999-08-25 2002-07-02 Immunex Corporation Methods and host cells for improved cell culture
EP2278021A3 (en) * 1999-11-01 2011-05-18 Novartis Vaccines and Diagnostics, Inc. Expression vectors, transfection systems, and method of use thereof
US6558948B1 (en) 1999-11-23 2003-05-06 Stefan Kochanek Permanent amniocytic cell line, its production and use for the production of gene transfer vectors
US6586205B1 (en) * 2000-02-11 2003-07-01 Millennium Pharmaceuticals, Inc. 43239 a novel GPCR-like molecule and uses thereof
WO2001059117A2 (en) * 2000-02-11 2001-08-16 Millennium Pharmaceuticals, Inc. Seven-transmembrane proteins/g-protein coupled receptors
CA2422533A1 (en) 2000-09-20 2002-03-28 Ml Laboratories Plc Artificial ubiquitous chromatin opening elements (ucoe)
JP4307079B2 (ja) 2001-01-26 2009-08-05 セレキス エスアー マトリックス付着領域およびその使用方法
US20020155540A1 (en) * 2001-02-14 2002-10-24 Malla Padidam Method to reduce transcriptional interference between tandem genes
FR2821855B1 (fr) * 2001-03-09 2004-04-02 Cayla Genes synthetiques et plasmides bacteriens depourvus de cpg
TWI377253B (en) * 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
WO2002099089A1 (en) 2001-06-04 2002-12-12 Corixa Corporation Compositions and methods for high-level, large-scale production of recombinant proteins
ATE466941T1 (de) * 2001-07-04 2010-05-15 Chromagenics Bv Dns-sequenzen mit anti-repressor-aktivität
US20040038304A1 (en) * 2002-03-28 2004-02-26 Gala Design, Inc. Antibody libraries
DE60336228D1 (de) * 2002-06-14 2011-04-14 Chromagenics Bv Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden
WO2003106674A2 (en) * 2002-06-14 2003-12-24 Chromagenics B.V. Means and methods for regulating gene expression
AU2003263161A1 (en) 2002-09-20 2004-04-08 Novo Nordisk A/S Method for making recombinant proteins
DK1573058T3 (da) * 2002-12-18 2012-01-16 Chromagenics Bv Fremgangsmåde til forbedring af proteinproduktion
EP1572994B1 (en) * 2002-12-20 2007-02-21 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
KR101269656B1 (ko) 2003-10-24 2013-05-30 셀렉시스 에스. 에이. Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현
ES2296201T3 (es) * 2004-07-08 2008-04-16 Chromagenics B.V. Secuencia novedosa para mejorar la expresion de acido nucleico.
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
NZ553700A (en) * 2004-11-08 2010-09-30 Chromagenics Bv Selection of host cells expressing protein at high levels using a selectable marker with a non-optimal start codon
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1996705B1 (en) * 2006-03-20 2011-08-31 ChromaGenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof

Also Published As

Publication number Publication date
KR20070090151A (ko) 2007-09-05
JP2008518613A (ja) 2008-06-05
WO2006048459A3 (en) 2006-08-10
CA2581422C (en) 2012-05-22
EP1809750B1 (en) 2012-03-21
BRPI0517380A (pt) 2008-10-07
MX2007004595A (es) 2007-08-15
EA010863B1 (ru) 2008-12-30
WO2006048459A2 (en) 2006-05-11
CA2581422A1 (en) 2006-05-11
IL183035A0 (en) 2007-09-20
HK1101264A1 (en) 2007-10-12
JP5291341B2 (ja) 2013-09-18
EP1809750A2 (en) 2007-07-25
NO344469B1 (no) 2019-12-30
AU2005300503B2 (en) 2010-12-16
NO20072845L (no) 2007-06-05
EA200701023A1 (ru) 2007-10-26
KR101271884B1 (ko) 2013-06-05
AU2005300503A1 (en) 2006-05-11
US20060141577A1 (en) 2006-06-29

Similar Documents

Publication Publication Date Title
HK1101264A1 (en) Selection of host cells expressing protein at high levels
HK1103944A1 (en) Novel canola protein isolate
EP1733039A4 (en) IMPROVED BACTERIA CELL FOR DIRECT EXPRESSION OF PEPTIDES
EP1766073A4 (en) IMPROVING PROTEIN EXPRESSION
GB0422901D0 (en) Lipocalin protein
GB0426182D0 (en) Photoporation of cells
GB0416830D0 (en) Host cell
GB0423974D0 (en) Proteins
GB0416845D0 (en) Host cell
GB0417887D0 (en) Protein
GB0416035D0 (en) Protein
GB0401882D0 (en) Protein
AU2004902617A0 (en) Protein Evolution
AU2004902367A0 (en) Protein Evolution
GB0415161D0 (en) Proteins
GB0418248D0 (en) Proteins
GB0412570D0 (en) Proteins
GB0411678D0 (en) Proteins
GB0418666D0 (en) Proteins
GB0423002D0 (en) Proteins
GB0405885D0 (en) Proteins
GB0405643D0 (en) Proteins
GB0413697D0 (en) Protein
GB0401311D0 (en) Protein
GB0402088D0 (en) Protein