NO20072845L - Seksjon av vertsceller som uttrykker hoye nivaer av proteiner - Google Patents

Seksjon av vertsceller som uttrykker hoye nivaer av proteiner

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Publication number
NO20072845L
NO20072845L NO20072845A NO20072845A NO20072845L NO 20072845 L NO20072845 L NO 20072845L NO 20072845 A NO20072845 A NO 20072845A NO 20072845 A NO20072845 A NO 20072845A NO 20072845 L NO20072845 L NO 20072845L
Authority
NO
Norway
Prior art keywords
polypeptide
interest
selectable marker
host cells
cells expressing
Prior art date
Application number
NO20072845A
Other languages
English (en)
Other versions
NO344469B1 (no
Inventor
Arie Pieter Otte
Theodorus Hendrikus Jaco Kwaks
Richard George Antonius Sewalt
Henricus Johannes Mar Blokland
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Publication of NO20072845L publication Critical patent/NO20072845L/no
Publication of NO344469B1 publication Critical patent/NO344469B1/no

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Oppfinnelsen skaffer til veie et DNA-molekyl som består av en multisistronisk transkripsjonsenhet som koder for i) et selektabelt markørpolypeptid som virker funksjonelt i en eukaryot vertscelle, og for ii) et polypeptid av interesse, der polypeptidet av interesse besitter en translasjons-initierings-sekvens separat fra den tilhørende det selektable markørpolypeptidet, karakterisert ved at den kodende sekvensne for polypeptidet av interesse befinner seg nedstrøms for den kodende sekvensen til den selektable markøren i nevnte multisistroniske transkripsjonsenhet, og der nukleinsyre-sekvensen som koder for det selektable markørpolypeptidet består av en mutasjon som senker translasjons-effektiviteten til den selektable markøren i en eukaryot vertscelle. Oppfinnelsen skaffer også til veie metoder for å oppnå vertsceller som uttrykker et polypeptid av interesse, der nevnte vertsceller består av DNA-molekylene, i henhold til den foreliggende oppfinnelsen. Oppfinnelsen sørger videre for produksjon av polypeptidet av interesse, og innebærer dyrking av vertsceller som består av DNA-molekylene, i henhold til den foreliggende oppfinnelsen. ?? ?? ?? ??
NO20072845A 2004-11-08 2007-06-05 Seksjon av vertsceller som uttrykker høye nivåer av proteiner NO344469B1 (no)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US62630104P 2004-11-08 2004-11-08
EP04105593 2004-11-08
US69661005P 2005-07-05 2005-07-05
PCT/EP2005/055794 WO2006048459A2 (en) 2004-11-08 2005-11-07 Selection of host cells expressing protein at high levels

Publications (2)

Publication Number Publication Date
NO20072845L true NO20072845L (no) 2007-06-05
NO344469B1 NO344469B1 (no) 2019-12-30

Family

ID=36090897

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20072845A NO344469B1 (no) 2004-11-08 2007-06-05 Seksjon av vertsceller som uttrykker høye nivåer av proteiner

Country Status (13)

Country Link
US (1) US20060141577A1 (no)
EP (1) EP1809750B1 (no)
JP (1) JP5291341B2 (no)
KR (1) KR101271884B1 (no)
AU (1) AU2005300503B2 (no)
BR (1) BRPI0517380A (no)
CA (1) CA2581422C (no)
EA (1) EA010863B1 (no)
IL (1) IL183035A0 (no)
MX (1) MX2007004595A (no)
NO (1) NO344469B1 (no)
PL (1) PL1809750T3 (no)
WO (1) WO2006048459A2 (no)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654442A (en) * 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
EP1573058B1 (en) * 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
WO2004056986A2 (en) 2002-12-20 2004-07-08 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
SI1809750T1 (sl) 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
RU2478709C2 (ru) 2005-07-21 2013-04-10 Эбботт Лэборетриз ЭКСПРЕССИЯ МНОЖЕСТВА ГЕНОВ, ВКЛЮЧАЯ sORF-КОНСТРУКЦИИ, И СПОСОБЫ ЭКСПРЕССИРОВАНИЯ ИММУНОГЛОБУЛИНА
PL1987150T3 (pl) * 2006-02-21 2011-10-31 Chromagenics Bv Selekcja komórek gospodarza wyrażających białka na wysokim poziomach
US7968700B2 (en) 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof
ES2388003T3 (es) * 2006-09-20 2012-10-05 Genzyme Corporation Un sistema basado en FACS y en la proteína indicadora para un desarrollo de alto rendimiento de proteínas terapéuticas
US20080274137A1 (en) * 2007-05-02 2008-11-06 Jean Christophe Francis Audonnet DNA plasmids having improved expression and stability
CA2716766A1 (en) 2008-02-27 2009-09-03 Fuso Pharmaceutical Industries, Ltd. Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof
EP2352821B1 (en) * 2008-09-08 2016-11-23 Cellectis Meganuclease variants cleaving a dna target sequence from a glutamine synthetase gene and uses thereof
CN102325881B (zh) * 2008-12-22 2014-08-20 国立大学法人北海道大学 用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用
WO2010147464A1 (en) 2009-06-15 2010-12-23 Cellagenics B.V. Use of a cysteine synthesizing enzyme as selectable marker
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
EP2582816B1 (en) 2010-06-15 2017-12-20 Cellagenics B.V. Novel intergenic elements for enhancing gene expression
CN103180443B (zh) 2010-09-01 2016-01-13 萨拉基尼克有限公司 用于增强基因表达的来自核糖体蛋白启动子的核酸片段
KR102366081B1 (ko) 2012-07-31 2022-02-23 에이지엑스 쎄라퓨틱스, 인크. Hla g-변형된 세포 및 방법
EP3421046A1 (en) 2014-11-04 2019-01-02 Janssen Vaccines & Prevention B.V. Therapeutic hpv16 vaccines
EA037295B1 (ru) 2015-08-20 2021-03-05 Янссен Вэксинс Энд Превеншн Б.В. Терапевтические вакцины против hpv18
US11635363B2 (en) 2015-10-09 2023-04-25 Genzyme Corporation FLARE (flow cytometry attenuated reporter expression) technology for rapid bulk sorting
BR112018072372A2 (pt) 2016-05-02 2019-02-19 Janssen Vaccines & Prevention B.V. combinações de vacina terapêutica contra o hpv
US20180119192A1 (en) 2016-10-07 2018-05-03 Genzyme Corporation Early post-transfection isolation of cells (epic) for biologics production
JP7264353B2 (ja) * 2018-05-24 2023-04-25 国立大学法人北海道大学 新規ベクターおよびその利用
JP7402453B2 (ja) * 2018-08-13 2023-12-21 Ｓｐｉｂｅｒ株式会社 細胞を単離又は同定する方法及び細胞集団
EP3856910B1 (en) * 2018-09-24 2024-11-20 Merck Sharp & Dohme LLC Expression vectors for eukaryotic expression systems
MX2023005938A (es) 2020-11-19 2023-05-29 Genzyme Corp Sistema de indicadores multiples de expresion atenuada de indicadores por citometria de flujo (flare) y sus metodos de uso.

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3700633A (en) * 1971-05-05 1972-10-24 Shell Oil Co Selectively hydrogenated block copolymers
US3821148A (en) * 1973-09-28 1974-06-28 Exxon Research Engineering Co Plasticized thermoplastic block copolymers
US4122134A (en) * 1974-02-13 1978-10-24 Sumitomo Chemical Company, Limited Method for producing transparent block copolymer resin
US3965830A (en) * 1974-12-05 1976-06-29 Usm Corporation Assembly for automatic bar tacking
US4267284A (en) * 1977-01-07 1981-05-12 Phillips Petroleum Company Tough, transparent articles from styrene polymers blended with certain block-random copolymers
US4965196A (en) 1983-01-19 1990-10-23 Genentech, Inc. Polycistronic expression vector construction
FR2569723B1 (fr) * 1984-08-31 1986-09-19 Centre Nat Rech Scient Application des antibiotiques de la famille des phleomycines a titre d'agent de selection dans le domaine du genie genetique
US4835200A (en) * 1986-12-19 1989-05-30 Shell Oil Company Color stable hot melt adhesive
US4882384A (en) * 1988-02-01 1989-11-21 Shell Oil Company Modified block copolymers
EP0484411A4 (en) * 1989-07-24 1992-06-17 Seragen, Inc. Prevention of internal initiation
US5256568A (en) * 1990-02-12 1993-10-26 Regeneron Phamaceuticals, Inc. Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers
DK0724639T3 (da) 1991-03-29 2001-03-05 Genentech Inc Fremgangsmåder til udvælgelse af rekombinante værtsceller, der udtrykker høje niveauer af et ønsket protein
US6319707B1 (en) * 1991-08-12 2001-11-20 Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington Cap-independent multicistronic retroviral vectors
JP2686025B2 (ja) * 1992-08-25 1997-12-08 理化学研究所 ブラストサイジンｓデアミナーゼ遺伝子
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
DE69332859T2 (de) 1992-11-13 2003-12-18 Idec Pharmaceuticals Corp., San Diego Konsensus-kozak-sequenzen zur säugetier-exprimierung
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
WO1994023046A1 (en) 1993-04-07 1994-10-13 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Dna sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5561053A (en) 1994-08-05 1996-10-01 Genentech, Inc. Method for selecting high-expressing host cells
US5627033A (en) * 1995-03-28 1997-05-06 Research & Diagnostics Systems, Inc. Mammalian expression vectors
IL160406A0 (en) 1995-06-15 2004-07-25 Crucell Holland Bv A cell harbouring nucleic acid encoding adenoritus e1a and e1b gene products
DE19523585A1 (de) * 1995-06-29 1997-01-02 Basf Ag Schlagzähe Formmasse aus Styrolpolymeren
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
DE19621688A1 (de) * 1996-05-30 1997-12-04 Basf Ag Transparente, nicht-blockende Folie
US6107477A (en) * 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences
CA2283253A1 (en) 1997-03-04 1998-09-11 Baxter International Inc. Adenovirus e1-complementing cell lines
US5888809A (en) * 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
DE19832245A1 (de) 1998-07-17 2000-01-20 Heidelberger Druckmasch Ag Verfahren zum Bedienen von Geräten in der Reproduktionstechnik
KR100795626B1 (ko) 1998-07-21 2008-01-17 코브라 바이오매뉴팩쳐링 피엘씨. 도처에 있는 염색질 개방 요소(유씨오이)를 포함하는폴리뉴클레오티드
ES2375266T3 (es) 1998-07-21 2012-02-28 Millipore Corporation Un polinucleótido que comprende un elemento de apertura de cromatina ubicuo (ucoe).
DE69936867T2 (de) * 1998-08-14 2008-04-30 Chromagenics B.V. Dns sequenz, verfahren für dessen nachweis und herstellung und benutzung
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6800457B2 (en) * 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2000023606A1 (en) * 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
IL145849A0 (en) 1999-04-15 2002-07-25 Crucell Holland Bv Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein
US6391981B1 (en) * 1999-04-23 2002-05-21 Kraton Polymers Us Llc Increased throughput in the manufacture of anionic polymers by reduction in polymer cement viscosity through the addition of metal alkyls
JP2003504016A (ja) 1999-06-30 2003-02-04 アメリカ合衆国 遺伝子発現の制御のための特異的エンハンサー遮断活性を有する絶縁体としてのｄｎａ結合タンパク質および配列
NZ529329A (en) * 1999-08-25 2005-06-24 Immunex Corp Compositions and methods for improved cell culture
EP2278022A3 (en) * 1999-11-01 2011-05-18 Novartis Vaccines and Diagnostics, Inc. Expression vectors, transfection systems, and method of use thereof
US6558948B1 (en) 1999-11-23 2003-05-06 Stefan Kochanek Permanent amniocytic cell line, its production and use for the production of gene transfer vectors
US6586205B1 (en) * 2000-02-11 2003-07-01 Millennium Pharmaceuticals, Inc. 43239 a novel GPCR-like molecule and uses thereof
AU2001238178A1 (en) * 2000-02-11 2001-08-20 Millennium Pharmaceuticals, Inc. Seven-transmembrane proteins/g-protein coupled receptors
CN1461343B (zh) 2000-09-20 2012-10-31 Emd密理博公司 多核苷酸
CA2435972C (en) 2001-01-26 2011-09-13 University Of Lausanne Matrix attachment regions and methods for use thereof
US20020155540A1 (en) * 2001-02-14 2002-10-24 Malla Padidam Method to reduce transcriptional interference between tandem genes
FR2821855B1 (fr) * 2001-03-09 2004-04-02 Cayla Genes synthetiques et plasmides bacteriens depourvus de cpg
TWI377253B (en) * 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
WO2002099089A1 (en) 2001-06-04 2002-12-12 Corixa Corporation Compositions and methods for high-level, large-scale production of recombinant proteins
ATE466943T1 (de) * 2001-07-04 2010-05-15 Chromagenics Bv Dns-sequenzen mit anti-repressor aktivität
US20040038304A1 (en) * 2002-03-28 2004-02-26 Gala Design, Inc. Antibody libraries
EP1513936A2 (en) * 2002-06-14 2005-03-16 Chromagenics B.V. Use of repression blocking sequences in methods for enhancing gene expression
NZ537073A (en) * 2002-06-14 2006-08-31 Chromagenics B A method for simultaneous production of multiple proteins; vectors and cells for use therein
AU2003263161A1 (en) 2002-09-20 2004-04-08 Novo Nordisk A/S Method for making recombinant proteins
EP1573058B1 (en) * 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
WO2004056986A2 (en) * 2002-12-20 2004-07-08 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
CA2535836C (en) 2003-10-24 2014-02-04 Nicolas Mermod High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of mar sequences
ES2296201T3 (es) * 2004-07-08 2008-04-16 Chromagenics B.V. Secuencia novedosa para mejorar la expresion de acido nucleico.
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
SI1809750T1 (sl) * 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
US7968700B2 (en) * 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof

Also Published As

Publication number Publication date
WO2006048459A3 (en) 2006-08-10
MX2007004595A (es) 2007-08-15
JP5291341B2 (ja) 2013-09-18
IL183035A0 (en) 2007-09-20
AU2005300503A1 (en) 2006-05-11
EP1809750A2 (en) 2007-07-25
AU2005300503B2 (en) 2010-12-16
EP1809750B1 (en) 2012-03-21
EA200701023A1 (ru) 2007-10-26
KR20070090151A (ko) 2007-09-05
EA010863B1 (ru) 2008-12-30
CA2581422C (en) 2012-05-22
WO2006048459A2 (en) 2006-05-11
BRPI0517380A (pt) 2008-10-07
US20060141577A1 (en) 2006-06-29
HK1101264A1 (en) 2007-10-12
JP2008518613A (ja) 2008-06-05
PL1809750T3 (pl) 2012-08-31
CA2581422A1 (en) 2006-05-11
NO344469B1 (no) 2019-12-30
KR101271884B1 (ko) 2013-06-05

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