CR10380A - Procesos de purificacion para aislar virus purificado de la estomatitis vesicular provenientes de cultivo celular. - Google Patents

Procesos de purificacion para aislar virus purificado de la estomatitis vesicular provenientes de cultivo celular.

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Publication number
CR10380A
CR10380A CR10380A CR10380A CR10380A CR 10380 A CR10380 A CR 10380A CR 10380 A CR10380 A CR 10380A CR 10380 A CR10380 A CR 10380A CR 10380 A CR10380 A CR 10380A
Authority
CR
Costa Rica
Prior art keywords
cell culture
vsv
purification processes
vesicular stomatitis
purified viruses
Prior art date
Application number
CR10380A
Other languages
English (en)
Inventor
Yun Kang
Mark William Cutler
Amadou Affrey Ouattara
Kristen Elissa Syvertsen
Original Assignee
Wyeth Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp filed Critical Wyeth Corp
Publication of CR10380A publication Critical patent/CR10380A/es

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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5252—Virus inactivated (killed)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5254—Virus avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20241—Use of virus, viral particle or viral elements as a vector
    • C12N2760/20243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20251—Methods of production or purification of viral material
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20251—Methods of production or purification of viral material
    • C12N2760/20252—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Son procesos de purificacion para obtener virus de estomatitis vasicular (VSV) novedosos de pureza mejorada. Mas particularmente, en ciertas modalidades, se describe un proceso para purificar VSV del fluido de cultivo celular de un cultivo celular mamifero infectado con VSV, el proceso comprende: clarificar el fluido de cultivo celular mediante centrifugacion a baja velocidad y recuperar el VSV en el sobrenadante; filtrar el sobrenadante a traves de un filtro de 0.2 a 0.45 pm y recuperar el VSV en la solucion filtrada.
CR10380A 2006-04-20 2008-10-20 Procesos de purificacion para aislar virus purificado de la estomatitis vesicular provenientes de cultivo celular. CR10380A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US79337606P 2006-04-20 2006-04-20

Publications (1)

Publication Number Publication Date
CR10380A true CR10380A (es) 2008-12-02

Family

ID=38625574

Family Applications (1)

Application Number Title Priority Date Filing Date
CR10380A CR10380A (es) 2006-04-20 2008-10-20 Procesos de purificacion para aislar virus purificado de la estomatitis vesicular provenientes de cultivo celular.

Country Status (19)

Country Link
US (1) US7875446B2 (es)
EP (1) EP2007883B1 (es)
JP (1) JP5129805B2 (es)
KR (1) KR101385927B1 (es)
CN (1) CN101426905B (es)
AT (1) ATE539149T1 (es)
AU (1) AU2007240797B2 (es)
BR (1) BRPI0710820A2 (es)
CA (1) CA2648792C (es)
CR (1) CR10380A (es)
DK (1) DK2007883T3 (es)
ES (1) ES2377356T3 (es)
IL (1) IL194629A (es)
MX (1) MX2008013494A (es)
PL (1) PL2007883T3 (es)
PT (1) PT2007883E (es)
RU (1) RU2484135C2 (es)
SI (1) SI2007883T1 (es)
WO (1) WO2007123961A2 (es)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103555670B (zh) * 2004-12-23 2015-08-12 米迪缪尼有限公司 用于病毒增殖的非致瘤性mdck细胞系
EP2069480B1 (en) 2006-09-15 2014-03-19 MedImmune, LLC Mdck cell lines supporting viral growth to high titers and bioreactor process using the same
EP2173861B2 (en) * 2007-06-15 2017-02-22 Amgen Inc. Methods of treating cell culture media for use in a bioreactor
ES2401162T3 (es) * 2007-10-02 2013-04-17 Ptc Phage Technology Center Gmbh Procedimiento y sistema para la purificación a escala industrial de bacteriófagos destinados para terapia con bacteriófagos
KR20110004354A (ko) * 2007-12-21 2011-01-13 와이어쓰 엘엘씨 유전자 변형된 약독화 수포성 구내염 바이러스, 이의 조성물 및 사용 방법
US9169491B2 (en) * 2008-06-18 2015-10-27 Oxford Biomedica (Uk) Limited Virus purification
CN103952376A (zh) * 2008-09-24 2014-07-30 米迪缪尼有限公司 培养细胞、增殖和纯化病毒的方法
KR20110074563A (ko) * 2008-09-24 2011-06-30 메디뮨 엘엘씨 바이러스의 정제 방법
EP2346891A1 (en) * 2008-10-17 2011-07-27 Percivia LLC. Clarification process at higher cell density
US20100143889A1 (en) * 2008-12-02 2010-06-10 Mayo Foundation For Medical Education And Research Rhabdoviridae virus preparations
JP5843615B2 (ja) * 2009-02-06 2016-01-13 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム 密度勾配超遠心分離によるウイルスまたはウイルス抗原の精製
MX2012004711A (es) 2009-10-20 2012-05-23 Abbott Lab Aislamiento y purificacion de los anticuerpos anti-il-13 al usar cromatografia de afinidad con proteina a.
CN102191226A (zh) * 2010-03-08 2011-09-21 北京清大天一科技有限公司 细胞培养混合物的分离纯化方法
CN103052399B (zh) 2010-08-12 2016-06-29 依生生物制药控股有限公司 用于减少病毒组合物中dna杂质的方法
US9044498B2 (en) 2010-12-02 2015-06-02 Oncolytics Biotech Inc. Lyophilized viral formulations
JP6034798B2 (ja) 2010-12-02 2016-11-30 オンコリティクス バイオテク，インコーポレーテッド 液体ウイルス製剤
JP6027104B2 (ja) * 2011-07-08 2016-11-16 イー・エム・デイー・ミリポア・コーポレイシヨン 使い捨てバイオテクノロジー方法のための改良されたデプスフィルター
US9610346B2 (en) 2012-03-23 2017-04-04 International Aids Vaccine Initiative Recombinant viral vectors
US9005888B2 (en) 2012-06-14 2015-04-14 System Biosciences, Llc Methods for microvesicle isolation and selective removal
CN110172405A (zh) * 2012-08-20 2019-08-27 泰尔茂比司特公司 浓缩穿过细胞生长腔室循环的流体的组分
AU2013306080B2 (en) * 2012-08-24 2019-01-31 Global Life Sciences Solutions Usa Llc Virus purification and formulation process
FR3010720B1 (fr) 2013-09-16 2017-08-11 Genethon Procede de production de virus enveloppes
FR3014901B1 (fr) * 2013-12-17 2017-06-09 Genethon Procede de purification de virus ou vecteurs viraux enveloppes
US10640531B2 (en) * 2014-05-21 2020-05-05 Unchained Labs Systems and methods for exchange of buffer solutions
CN106857498B (zh) * 2016-12-27 2021-05-14 科兴（大连）疫苗技术有限公司 一种不含二甲基亚砜的细胞冻存保护液及其应用
CN107964037A (zh) * 2017-05-16 2018-04-27 浙江海隆生物科技有限公司 基于cho细胞表达系统的重组猪瘟e2蛋白的纯化方法及应用
CN110317832B (zh) 2018-03-28 2022-07-05 西比曼生物科技（香港）有限公司 Gmp级规模化制备重组慢病毒载体的纯化制剂的方法
CN110317791A (zh) 2018-03-29 2019-10-11 西比曼生物科技（香港）有限公司 Gmp级无血清悬浮细胞大规模生产慢病毒的方法
EP3823677A4 (en) * 2018-07-19 2022-06-01 Helixmith Co., Ltd. FREEZE-DRIED PHARMACEUTICAL COMPOSITIONS INTENDED FOR NAKED-DNA GENE THERAPY
CA3107462A1 (en) * 2018-07-24 2020-01-30 Voyager Therapeutics, Inc. Systems and methods for producing gene therapy formulations
TW202043466A (zh) 2019-01-25 2020-12-01 德商百靈佳殷格翰國際股份有限公司 編碼ccl21之重組棒狀病毒
CN110305850A (zh) * 2019-05-15 2019-10-08 苏州奥特铭医药科技有限公司 一种利用293细胞生产制备溶瘤病毒的方法
BR112022025251A2 (pt) * 2020-07-10 2023-01-17 Boehringer Ingelheim Int Processo para produzir rabdovírus purificado a partir de cultura celular

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4556556A (en) * 1984-03-23 1985-12-03 Advanced Genetics Research Institute Vaccine for the prevention of vesicular stomatitis virus infection
ES2210273T5 (es) 1994-07-18 2010-03-29 Conzelmann, Karl-Klaus, Prof. Dr. Virus con arn de cadena negativa no segmentado recombinante infeccioso.
US7153510B1 (en) 1995-05-04 2006-12-26 Yale University Recombinant vesiculoviruses and their uses
FR2737730B1 (fr) 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
US6261823B1 (en) * 1996-12-13 2001-07-17 Schering Corporation Methods for purifying viruses
EP1032269B1 (en) * 1997-10-09 2007-08-29 Wellstat Biologics Corporation Treatment of neoplasms with interferon-sensitive, clonal viruses
KR100991683B1 (ko) * 1998-02-17 2010-11-04 쉐링 코포레이션 바이러스 제제의 농축방법
AU2002362761A1 (en) * 2001-10-09 2003-04-22 University Of Miami Generation of virus-like particles by vsv
US20030180936A1 (en) 2002-03-15 2003-09-25 Memarzadeh Bahram Eric Method for the purification, production and formulation of oncolytic adenoviruses
US20030175688A1 (en) 2002-03-15 2003-09-18 Rukmini Pennathur-Das Method for the purification and production of oncolytic adenoviruses
WO2003093463A1 (en) * 2002-04-30 2003-11-13 Oncolytics Biotech Inc. Improved viral purification methods
US20050196854A1 (en) * 2002-05-14 2005-09-08 Konz John O.Jr. Methods of adenovirus purification
US20050009168A1 (en) 2003-05-12 2005-01-13 Robbins Joan Marie Methods and apparatus for Adeno associated virus purification
DE102004004043B4 (de) 2004-01-27 2013-04-18 Apanovis Biotechnologie Gmbh Reinigung von hochmolekularen Verbindungen mittels Affinitätsmembranchromatographie
CN1922308A (zh) * 2004-02-23 2007-02-28 克鲁塞尔荷兰公司 病毒纯化方法
KR101255016B1 (ko) * 2004-04-09 2013-04-17 와이어쓰 엘엘씨 소포성 구내염 바이러스의 상승적 감쇠, 그의 벡터 및 그의 면역원성 조성물
US20090042274A1 (en) 2004-07-27 2009-02-12 Genomidea Inc. Method of Purifying Virus Envelope

Also Published As

Publication number Publication date
DK2007883T3 (da) 2012-02-06
RU2008140137A (ru) 2010-05-27
AU2007240797A1 (en) 2007-11-01
RU2484135C2 (ru) 2013-06-10
ATE539149T1 (de) 2012-01-15
MX2008013494A (es) 2009-01-26
CN101426905A (zh) 2009-05-06
CN101426905B (zh) 2013-03-27
SI2007883T1 (sl) 2012-02-29
KR20080111544A (ko) 2008-12-23
ES2377356T3 (es) 2012-03-26
WO2007123961A3 (en) 2008-03-06
JP5129805B2 (ja) 2013-01-30
US20070249019A1 (en) 2007-10-25
KR101385927B1 (ko) 2014-04-24
IL194629A (en) 2013-11-28
PT2007883E (pt) 2012-02-17
IL194629A0 (en) 2011-08-01
EP2007883A2 (en) 2008-12-31
JP2009534030A (ja) 2009-09-24
PL2007883T3 (pl) 2012-07-31
EP2007883B1 (en) 2011-12-28
US7875446B2 (en) 2011-01-25
AU2007240797B2 (en) 2013-05-23
CA2648792A1 (en) 2007-11-01
CA2648792C (en) 2016-06-21
BRPI0710820A2 (pt) 2011-08-23
WO2007123961A2 (en) 2007-11-01

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