CN207294780U - A kind of classification inoculation apparatus of fermentation tank - Google Patents

A kind of classification inoculation apparatus of fermentation tank Download PDF

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Publication number
CN207294780U
CN207294780U CN201721231024.3U CN201721231024U CN207294780U CN 207294780 U CN207294780 U CN 207294780U CN 201721231024 U CN201721231024 U CN 201721231024U CN 207294780 U CN207294780 U CN 207294780U
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China
Prior art keywords
tube
fermentation tank
inoculated
classification inoculation
strain transfer
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CN201721231024.3U
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Chinese (zh)
Inventor
谭宏宇
张宏达
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YICHANG HEC CHANGJIANG PHARMACEUTICAL Co.,Ltd.
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Guangdong HEC Pharmaceutical
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Abstract

The utility model belongs to technical field of biological fermentation, more particularly to a kind of classification inoculation apparatus of fermentation tank, the classification inoculation apparatus includes strain transfer tube, inoculated tube and cleaning mouthpiece, described strain transfer tube one end is connected with fermentation tank, the other end is connected with inoculated tube and cleaning mouthpiece, the strain transfer tube and the angle of fermentation tank joint and horizontal plane are more than 0 °, less than 90 °.The classification inoculation apparatus is off-loadable when not being inoculated with, and is blocked the hole that connects in fermentation tank with blind plate.This classification inoculation apparatus need not open fermentation tank when being inoculated with, it can be achieved that online sterilizing and on-line cleaning, simple in structure, easy to use, greatly microbiological contamination can be avoided to pollute, have good application prospect.

Description

A kind of classification inoculation apparatus of fermentation tank
Technical field
The utility model belongs to technical field of biological fermentation, is specifically related to a kind of fermentation classification inoculation apparatus.
Background technology
At present, the vaccination ways of prevalence are to use four valve groups in the world:When Shaking culture terminates, in superclean bench or life In thing safety cabinet, the bacterium solution in shaking flask is poured into the container in four valve groups, by container closure, then four valve groups are installed to waiting Kind stainless cylinder of steel pipeline on, then sterilize to stainless cylinder of steel, after sterilizing cooling, then open container and stainless cylinder of steel it Between valve, make bacterium solution flow into stainless cylinder of steel inside, complete inoculation.In addition, the common vaccination ways in domestic laboratory are flame Inoculation, the common vaccination ways of factory are inoculated with for flame laminar flow.
The shortcomings that four valve group vaccination ways is, bacterium solution must be placed in the container in four valve groups 1 it is small when or so, influence Bacterial concentration and activity;The shortcomings that flame inoculation and flame laminar flow are inoculated with is that both are non-closed systems, and it is miscellaneous to there is introducing The risk of bacterium.
Utility model content
The utility model aim is to avoid above-mentioned vaccination ways in risk present on inoculation technique, there is provided one kind inoculation Device, this classification inoculation apparatus need not open fermentation tank when being inoculated with, it can be achieved that online sterilizing and on-line cleaning, simple in structure, uses It is convenient, greatly microbiological contamination can be avoided to pollute.
The utility model is to avoid the technical solution used by above-mentioned risk from being:
A kind of classification inoculation apparatus of fermentation tank, it is characterised in that the classification inoculation apparatus includes strain transfer tube, inoculated tube (4) And cleaning mouthpiece (5), described strain transfer tube one end are connected with fermentation tank (1), the other end connects with inoculated tube (4) and cleaning Mouth pipe (5) is connected, and the angle of strain transfer tube and fermentation tank (1) joint and horizontal plane is more than 0 °, less than 90 °.
Preferably, the strain transfer tube is a straight tube (3).
Preferably, the strain transfer tube is a U-tube (8) and a straight tube (3).
Further, the strain transfer tube be connected to fermentation tank (1) height 80% and more than.
Further, the strain transfer tube is equipped with diaphragm valve (2), the strain transfer tube at nearly fermentation tank (1) end It is connected with one end of the diaphragm valve (2) by clip (6), fermentation tank (1) and the other end of the diaphragm valve (2) pass through clip (6) it is connected.
Further, the inoculated tube (4) with the strain transfer tube by being weldingly connected, it is described cleaning mouthpiece (5) It is connected with the strain transfer tube by clip (6).
Further, the inoculated tube (4) and described cleaning mouthpiece (5) upper surface are respectively equipped with a blind plate (7), described Inoculated tube (4) and cleaning mouthpiece (5) upper surface are connected with the blind plate (7) by clip (6), the cleaning mouthpiece In in set thermometer (9).
Further, inoculated tube (4) bore mutually agrees with inoculation bottle bore, and the inoculated tube (4) is highly less than etc. In 3 times of inoculated tube (4) diameters.
Preferably, the classification inoculation apparatus is through between Shaking culture and sweathouse.
Further, the inoculated tube (4) and the cleaning mouthpiece (5) are in clean unit.
The utility model has the advantages that:
Easy to operate, when inoculation, need not open fermentation tank, the risk of infection miscellaneous bacteria be reduced, in preferred embodiments of the present invention In, classification inoculation apparatus can run through between sweathouse and culture, and inoculation can be completed directly between culture, cut down the number of intermediate links, and reduce Risk, can also realize that on-line cleaning and online sterilizing require, largely avoid microbiological contamination pollution, the classification inoculation apparatus is not connecing It is off-loadable during kind, the hole that connects in fermentation tank is blocked with blind plate, application is strong.
Brief description of the drawings
Fig. 1 is classification inoculation apparatus structure diagram.
Legend:Fermentation tank -1, diaphragm valve -2, straight tube -3, inoculated tube -4, cleaning mouthpiece -5, clip -6, blind plate -7 are U-shaped Pipe -8, thermometer -9.
Embodiment
The utility model, can be with technical solution according to the present utility model and actual conditions from the limitation of following embodiments To determine specific embodiment.
Embodiment 1
In the present embodiment, there is a kind of inoculating device of fermentation tank fermentation tank 1, strain transfer tube 3, inoculated tube 4, cleaning to connect Mouth pipe 5, clip 6 and blind plate 7, strain transfer tube 3 are connected to 85% position of 1 height of fermentation tank, 3 one end of strain transfer tube Be connected with fermentation tank 1, the other end and inoculated tube 4 and cleaning mouthpiece 5 are connected, strain transfer tube 3 and 1 connecting place of fermentation tank and The angle of horizontal plane is 5 °.Inoculated tube 4 and 5 upper surface of cleaning mouthpiece are respectively equipped with a blind plate 7, inoculated tube 4 and cleaning interface The upper surface of pipe 5 is connected with blind plate 7 by clip 6.Recirculated water is cleaned since flowing into cleaning mouthpiece 5, high after cleaning Temperature sterilizing.When preparing to be inoculated with, the blind plate 7 of 4 upper surface of inoculated tube is removed, the ventilated membrane of inoculation bottle bottleneck is thrown off, by inoculation bottle It is upside down in inoculated tube 4, inoculation bottle seed liquor is flowed into fermentation tank 1 by gravity along strain transfer tube, completes inoculation.
Embodiment 2
In the present embodiment, a kind of inoculating device of fermentation tank has fermentation tank 1, strain transfer tube 3, diaphragm valve 2, inoculated tube 4th, mouthpiece 5, clip 6 and blind plate 7 are cleaned, adjacent with sweathouse between factory's fermentation plant, Shaking culture, sweathouse leans on wall There is a stainless steel fermentation tank 1 in wall side, and strain transfer tube 3 is through between Shaking culture and sweathouse, 3 one end of strain transfer tube It is connected with fermentation tank 1, the other end is connected with inoculated tube 4 and cleaning mouthpiece 5, and strain transfer tube 3 is connected to the height of fermentation tank 1 80% position, strain transfer tube 3 is equipped with diaphragm valve 2 at nearly 1 end of fermentation tank, and 2 one end of diaphragm valve passes through card with strain transfer tube Hoop 6 is connected, and the other end is connected with fermentation tank 1 by clip 6, strain transfer tube 3 and 1 connecting place of fermentation tank and horizontal plane Angle is 85 °.Inoculated tube 4 and cleaning mouthpiece 5 are in local A grades of environment between Shaking culture, inoculated tube 4 and cleaning interface 5 upper surface of pipe is respectively equipped with a blind plate 7, and inoculated tube 4 and cleaning 5 upper surface of mouthpiece are connected by clip 6 with blind plate 7, be inoculated with 4 bore of pipe mutually agrees with inoculation bottle bore, highly 3 times for 4 diameter of mouthpiece.Strain transfer tube 3 and stainless steel fermentation tank 1 one Rise and carry out on-line cleaning and online sterilizing, and keep positive pressure.During cleaning, the blind plate 7 of 5 upper surface of cleaning mouthpiece, circulation are opened Water is cleaned since flowing into cleaning mouthpiece 5, high-temperature sterilization after cleaning.The temperature cleaned in mouthpiece 5 can be utilized during sterilizing Whether degree 9 thermometrics of meter, observation temperature meet that sterilizing requires.When preparing to be inoculated with, the blind plate 7 of 4 upper surface of inoculated tube is removed, is thrown off The ventilated membrane of supporting inoculation bottle bottleneck, is tightened after inoculation bottle is upside down in inoculated tube 4 with 4 bore of inoculated tube, inoculation bottle Seed liquor is flowed into fermentation tank 1 by gravity along strain transfer tube 3, completes inoculation.
3 attached drawing of embodiment shows a kind of inoculating device of fermentation tank structure of the utility model, and there is fermentation tank 1, strain to pass Defeated pipe (including U-tube 8 and straight tube 3), diaphragm valve 2, inoculated tube 4, cleaning mouthpiece 5, clip 6, blind plate 7 and thermometer 8, Factory's fermentation plant, adjacent with sweathouse between Shaking culture, sweathouse has a stainless steel fermentation tank 1, strain by wall side Transfer tube (including U-tube 8 and straight tube 3) is through between Shaking culture and sweathouse, strain transfer tube (including U-tube 8 and straight tube 3) one end is connected with fermentation tank 1, and the other end is connected with inoculated tube 4 and cleaning mouthpiece 5, strain transfer tube (including 8 He of U-tube Straight tube 3) 90% position of 1 height of fermentation tank is connected to, strain transfer tube (including U-tube 8 and straight tube 3) is in nearly fermentation tank 1 End is equipped with diaphragm valve 2, and 2 one end of diaphragm valve is connected with strain transfer tube by clip 6, and the other end passes through clip 6 with fermentation tank 1 It is connected, strain transfer tube (including U-tube 8 and straight tube 3) and the angle of 1 connecting place of fermentation tank and horizontal plane are 45 °, inoculated tube 4 and cleaning mouthpiece 5 be in local A grade environment between Shaking culture, inoculated tube 4 is set respectively with mouthpiece 5 upper surface is cleaned There is a blind plate 7, inoculated tube 4 and clean the upper surface of mouthpiece 5 and be connected with blind plate 7 by clip 6,4 bore of inoculated tube and be inoculated with Bottleneck footpath is mutually agreed with, highly 2.5 times for 4 diameter of mouthpiece.Strain transfer tube (including U-tube 8 and straight tube 3) is sent out with stainless steel Fermentation tank 1 carries out on-line cleaning and online sterilizing together, and keeps positive pressure.During cleaning, the blind plate of 5 upper surface of cleaning mouthpiece is opened 7, recirculated water is cleaned since flowing into cleaning mouthpiece 5, high-temperature sterilization after cleaning.It can be utilized during sterilizing and clean mouthpiece 5 In 9 thermometric of thermometer, observation temperature whether meet sterilizing require.When preparing to be inoculated with, the membrane in diaphragm valve 2 is opened Plate, removes the blind plate 7 of 4 upper surface of inoculated tube, throws off the ventilated membrane of the inoculation bottle bottleneck supporting with 4 bore of inoculated tube, will be inoculated with Bottle is tightened after being upside down in inoculated tube 4, and inoculation bottle seed liquor is by gravity along strain transfer tube (including U-tube 8 and straight tube 3) Flow into fermentation tank 1, complete inoculation.In offline cleaning, cleaning mouthpiece 5 can be disassembled cleaning.Diaphragm valve 2 is long-term Damaged during use, can be dismantled and be replaced by clip 6.

Claims (10)

  1. A kind of 1. classification inoculation apparatus of fermentation tank, it is characterised in that the classification inoculation apparatus include strain transfer tube, inoculated tube (4) with And cleaning mouthpiece (5), described strain transfer tube one end are connected with fermentation tank (1), the other end and inoculated tube (4) and cleaning interface Manage (5) to be connected, the angle of strain transfer tube and fermentation tank (1) joint and horizontal plane is more than 0 °, less than 90 °.
  2. 2. classification inoculation apparatus according to claim 1, it is characterised in that the strain transfer tube is a straight tube (3).
  3. 3. classification inoculation apparatus according to claim 1, it is characterised in that the strain transfer tube is for a U-tube (8) and always Manage (3).
  4. 4. classification inoculation apparatus according to claim 1, it is characterised in that the strain transfer tube is connected to fermentation tank (1) height Degree 80% and more than.
  5. 5. classification inoculation apparatus according to claim 1, it is characterised in that the strain transfer tube is set at nearly fermentation tank (1) end There is diaphragm valve (2), the strain transfer tube is connected with one end of the diaphragm valve (2) by clip (6), fermentation tank (1) and institute The other end for stating diaphragm valve (2) is connected by clip (6).
  6. 6. classification inoculation apparatus according to claim 1, it is characterised in that the inoculated tube (4) is led to the strain transfer tube Cross and be weldingly connected, the cleaning mouthpiece (5) is connected with the strain transfer tube by clip (6), in the cleaning mouthpiece Equipped with internal thermometer (9).
  7. 7. classification inoculation apparatus according to claim 1, it is characterised in that the inoculated tube (4) and the cleaning mouthpiece (5) Upper surface is respectively equipped with a blind plate (7), the inoculated tube (4) and cleaning mouthpiece (5) upper surface and the blind plate (7) is logical Clip (6) is crossed to be connected.
  8. 8. classification inoculation apparatus according to claim 1, it is characterised in that inoculated tube (4) bore and inoculation bottle bore phase Agree with, the inoculated tube (4) is highly less than or equal to 3 times of inoculated tube (4) diameters.
  9. 9. according to the classification inoculation apparatus described in claim 1-8 any one claims, it is characterised in that the classification inoculation apparatus passes through It is through between Shaking culture and sweathouse.
  10. 10. classification inoculation apparatus according to claim 9, it is characterised in that the inoculated tube (4) and the cleaning mouthpiece (5) it is in clean unit.
CN201721231024.3U 2017-09-22 2017-09-22 A kind of classification inoculation apparatus of fermentation tank Active CN207294780U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201721231024.3U CN207294780U (en) 2017-09-22 2017-09-22 A kind of classification inoculation apparatus of fermentation tank

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201721231024.3U CN207294780U (en) 2017-09-22 2017-09-22 A kind of classification inoculation apparatus of fermentation tank

Publications (1)

Publication Number Publication Date
CN207294780U true CN207294780U (en) 2018-05-01

Family

ID=62442537

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201721231024.3U Active CN207294780U (en) 2017-09-22 2017-09-22 A kind of classification inoculation apparatus of fermentation tank

Country Status (1)

Country Link
CN (1) CN207294780U (en)

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Date Code Title Description
GR01 Patent grant
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract

Assignee: YICHANG HEC CHANGJIANG PHARMACEUTICAL Co.,Ltd.

Assignor: SUNSHINE LAKE PHARMA Co.,Ltd.

Contract record no.: X2020980002998

Denomination of utility model: Inoculation device of fermentation cylinder

Granted publication date: 20180501

License type: Exclusive License

Record date: 20200611

EE01 Entry into force of recordation of patent licensing contract
EC01 Cancellation of recordation of patent licensing contract

Assignee: YICHANG HEC CHANGJIANG PHARMACEUTICAL Co.,Ltd.

Assignor: SUNSHINE LAKE PHARMA Co.,Ltd.

Contract record no.: X2020980002998

Date of cancellation: 20200824

EC01 Cancellation of recordation of patent licensing contract
TR01 Transfer of patent right

Effective date of registration: 20200916

Address after: 443300, No. 38, Binjiang Road, Yidu, Hubei, Yichang

Patentee after: YICHANG HEC CHANGJIANG PHARMACEUTICAL Co.,Ltd.

Address before: 523808 Guangdong city of Dongguan province Hubei Songshan Industrial Park Industrial Road No. 1

Patentee before: SUNSHINE LAKE PHARMA Co.,Ltd.

TR01 Transfer of patent right