CN1942579B - 将氧化还原活性氨基酸位点特异地掺入蛋白质 - Google Patents

将氧化还原活性氨基酸位点特异地掺入蛋白质 Download PDF

Info

Publication number
CN1942579B
CN1942579B CN2004800301376A CN200480030137A CN1942579B CN 1942579 B CN1942579 B CN 1942579B CN 2004800301376 A CN2004800301376 A CN 2004800301376A CN 200480030137 A CN200480030137 A CN 200480030137A CN 1942579 B CN1942579 B CN 1942579B
Authority
CN
China
Prior art keywords
trna
quadrature
cell
amino acids
codon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2004800301376A
Other languages
English (en)
Chinese (zh)
Other versions
CN1942579A (zh
Inventor
L·阿方达
P·G·舒尔茨
张致文
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Institute filed Critical Scripps Research Institute
Publication of CN1942579A publication Critical patent/CN1942579A/zh
Application granted granted Critical
Publication of CN1942579B publication Critical patent/CN1942579B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN2004800301376A 2003-10-14 2004-10-13 将氧化还原活性氨基酸位点特异地掺入蛋白质 Expired - Fee Related CN1942579B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51153203P 2003-10-14 2003-10-14
US60/511,532 2003-10-14
PCT/US2004/034089 WO2005038002A2 (en) 2003-10-14 2004-10-13 Site-specific incorporation of redox active amino acids into proteins

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN200910004743A Division CN101532046A (zh) 2003-10-14 2004-10-13 将氧化还原活性氨基酸位点特异地掺入蛋白质

Publications (2)

Publication Number Publication Date
CN1942579A CN1942579A (zh) 2007-04-04
CN1942579B true CN1942579B (zh) 2013-06-26

Family

ID=34465242

Family Applications (2)

Application Number Title Priority Date Filing Date
CN2004800301376A Expired - Fee Related CN1942579B (zh) 2003-10-14 2004-10-13 将氧化还原活性氨基酸位点特异地掺入蛋白质
CN200910004743A Pending CN101532046A (zh) 2003-10-14 2004-10-13 将氧化还原活性氨基酸位点特异地掺入蛋白质

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN200910004743A Pending CN101532046A (zh) 2003-10-14 2004-10-13 将氧化还原活性氨基酸位点特异地掺入蛋白质

Country Status (14)

Country Link
US (5) US8008041B2 (enExample)
EP (1) EP1687401B1 (enExample)
JP (1) JP5657850B2 (enExample)
CN (2) CN1942579B (enExample)
AU (1) AU2004282577B2 (enExample)
BR (1) BRPI0415308A (enExample)
CA (1) CA2541166A1 (enExample)
DK (1) DK1687401T3 (enExample)
ES (1) ES2396712T3 (enExample)
IL (3) IL174947A (enExample)
MX (1) MXPA06004087A (enExample)
PL (1) PL1687401T3 (enExample)
PT (1) PT1687401E (enExample)
WO (1) WO2005038002A2 (enExample)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
CA2500653A1 (en) * 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
ATE542894T1 (de) 2003-04-17 2012-02-15 Scripps Research Inst Ausweitung des eukaryotischen genetischen codes
EP2392923B1 (en) 2003-06-18 2014-05-21 The Scripps Research Institute Method of producing proteins comprising unnatural amino acid
JP5192150B2 (ja) 2003-07-07 2013-05-08 ザ スクリプス リサーチ インスティチュート 直交リシルｔＲＮＡとアミノアシルｔＲＮＡシンテターゼの対の組成物及びその使用
CA2567624A1 (en) * 2004-05-25 2005-12-08 The Scripps Research Institute Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
DE102004031258A1 (de) * 2004-06-29 2006-02-09 Jennissen, Herbert P., Prof. Dr. Proteinhybride mit polyhydroxyaromatischen Aminosäure-Epitopen
US20060110784A1 (en) * 2004-09-22 2006-05-25 The Scripps Research Institute Site-specific labeling of proteins for NMR studies
MX2007004892A (es) 2004-10-27 2007-08-16 Scripps Research Inst Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales.
AU2006304110B2 (en) * 2005-10-12 2012-02-02 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
US7776535B2 (en) * 2006-02-06 2010-08-17 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
WO2007103490A2 (en) * 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
US7790847B2 (en) 2006-03-16 2010-09-07 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
KR20090024706A (ko) * 2006-05-23 2009-03-09 더 스크립스 리서치 인스티튜트 유전자적으로 코딩된 형광 쿠마린 아미노산
PL2052083T3 (pl) 2006-09-21 2014-06-30 Scripps Research Inst Genetycznie programowana ekspresja selektywnie siarczanowych białek u eubakterii
JP2010506591A (ja) 2006-10-18 2010-03-04 ザ スクリップス リサーチ インスティテュート 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み
WO2008137452A1 (en) * 2007-05-04 2008-11-13 Kenergy Royalty Company, Llc Implantable high efficiency digital stimulation device
US20090208994A1 (en) * 2007-10-25 2009-08-20 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
EP2217702A4 (en) * 2007-11-02 2012-05-16 Scripps Research Inst DIRECTED EVOLUTION USING PROTEINS WITH NON-NATURAL AMINO ACIDS
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
BRPI0819929A2 (pt) * 2007-12-11 2014-10-14 Scripps Research Inst Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotrófica
WO2009099672A2 (en) * 2008-02-08 2009-08-13 The Scripps Research Institute Breaking immunological tolerance with a genetically encoded unnatural amino acid
EP2373224A1 (en) * 2008-12-09 2011-10-12 Wilson-Cook Medical Inc. Apparatus and methods for controlled release of tacking devices
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
EP2394323B1 (en) * 2009-02-04 2017-05-24 Ben-gurion University Of The Negev Research And Development Authority Systems and methods for bio-electricity production
US10233249B2 (en) 2013-03-19 2019-03-19 Beijing Shenogen Pharma Group Ltd. Antibodies and methods for treating estrogen receptor-associated diseases
US20150331624A1 (en) * 2014-05-19 2015-11-19 Kabushiki Kaisha Toshiba Host-controlled flash translation layer snapshot
KR101956042B1 (ko) * 2017-07-13 2019-03-08 서강대학교산학협력단 표적 단백질 내 l-디하이드록시페닐알라닌의 도입 방법
US12134725B2 (en) 2019-06-17 2024-11-05 Merck Patent Gmbh Liquid crystal-based light valve

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1231697A (zh) * 1996-08-29 1999-10-13 麻省理工学院 体内蛋白质合成的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
CA2500653A1 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
DK1578949T3 (da) 2002-10-16 2009-07-27 Scripps Research Inst Stedspecifik optagelse af keto-aminnosyrer hos proteiner
MXPA05006836A (es) 2002-12-22 2005-08-16 Scripps Research Inst Colecciones ordenadas de proteinas.
ATE542894T1 (de) 2003-04-17 2012-02-15 Scripps Research Inst Ausweitung des eukaryotischen genetischen codes
EP2392923B1 (en) 2003-06-18 2014-05-21 The Scripps Research Institute Method of producing proteins comprising unnatural amino acid
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
JP5192150B2 (ja) 2003-07-07 2013-05-08 ザ スクリプス リサーチ インスティチュート 直交リシルｔＲＮＡとアミノアシルｔＲＮＡシンテターゼの対の組成物及びその使用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1231697A (zh) * 1996-08-29 1999-10-13 麻省理工学院 体内蛋白质合成的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Q57834(登录号).EBI,1997, *

Also Published As

Publication number Publication date
US20110008782A1 (en) 2011-01-13
BRPI0415308A (pt) 2006-12-05
WO2005038002A3 (en) 2006-11-23
EP1687401B1 (en) 2012-11-21
JP5657850B2 (ja) 2015-01-21
DK1687401T3 (da) 2013-02-04
ES2396712T3 (es) 2013-02-25
AU2004282577B2 (en) 2009-10-29
IL174947A0 (en) 2006-08-20
CN101532046A (zh) 2009-09-16
CA2541166A1 (en) 2005-04-28
US8114654B2 (en) 2012-02-14
US20120100570A1 (en) 2012-04-26
US20090298124A1 (en) 2009-12-03
WO2005038002A2 (en) 2005-04-28
MXPA06004087A (es) 2006-06-27
JP2007508034A (ja) 2007-04-05
US20070009990A1 (en) 2007-01-11
EP1687401A2 (en) 2006-08-09
CN1942579A (zh) 2007-04-04
PL1687401T3 (pl) 2013-04-30
IL174947A (en) 2011-11-30
US8008041B2 (en) 2011-08-30
US7494796B2 (en) 2009-02-24
US20050227318A1 (en) 2005-10-13
IL206889A0 (en) 2010-12-30
PT1687401E (pt) 2013-02-26
EP1687401A4 (en) 2007-08-01
US7811801B2 (en) 2010-10-12
IL201705A0 (en) 2011-08-01
AU2004282577A1 (en) 2005-04-28
US9783798B2 (en) 2017-10-10

Similar Documents

Publication Publication Date Title
CN1942579B (zh) 将氧化还原活性氨基酸位点特异地掺入蛋白质
CN101405401B (zh) 含非天然氨基酸苯基硒代半胱氨酸的蛋白质的遗传编程表达
CN101151366B (zh) 将炔基氨基酸体内掺入真细菌的蛋白
CN102827827B (zh) 体内掺入非天然氨基酸的正交翻译组分
JP5192150B2 (ja) 直交リシルｔＲＮＡとアミノアシルｔＲＮＡシンテターゼの対の組成物及びその使用
KR20090094812A (ko) 포유동물 세포에서 비천연 아미노산의 단백질 내로의 유전적 도입
EP1914314A2 (en) Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20130626

Termination date: 20151013

EXPY Termination of patent right or utility model