CA2541166A1 - Site-specific incorporation of redox active amino acids into proteins - Google Patents

Site-specific incorporation of redox active amino acids into proteins Download PDF

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Publication number
CA2541166A1
CA2541166A1 CA002541166A CA2541166A CA2541166A1 CA 2541166 A1 CA2541166 A1 CA 2541166A1 CA 002541166 A CA002541166 A CA 002541166A CA 2541166 A CA2541166 A CA 2541166A CA 2541166 A1 CA2541166 A1 CA 2541166A1
Authority
CA
Canada
Prior art keywords
trna
amino acid
cell
orthogonal
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002541166A
Other languages
English (en)
French (fr)
Inventor
Lital Alfonta
Peter G. Schultz
Zhiwen Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2541166A1 publication Critical patent/CA2541166A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA002541166A 2003-10-14 2004-10-13 Site-specific incorporation of redox active amino acids into proteins Abandoned CA2541166A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51153203P 2003-10-14 2003-10-14
US60/511,532 2003-10-14
PCT/US2004/034089 WO2005038002A2 (en) 2003-10-14 2004-10-13 Site-specific incorporation of redox active amino acids into proteins

Publications (1)

Publication Number Publication Date
CA2541166A1 true CA2541166A1 (en) 2005-04-28

Family

ID=34465242

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002541166A Abandoned CA2541166A1 (en) 2003-10-14 2004-10-13 Site-specific incorporation of redox active amino acids into proteins

Country Status (14)

Country Link
US (5) US8008041B2 (enExample)
EP (1) EP1687401B1 (enExample)
JP (1) JP5657850B2 (enExample)
CN (2) CN101532046A (enExample)
AU (1) AU2004282577B2 (enExample)
BR (1) BRPI0415308A (enExample)
CA (1) CA2541166A1 (enExample)
DK (1) DK1687401T3 (enExample)
ES (1) ES2396712T3 (enExample)
IL (3) IL174947A (enExample)
MX (1) MXPA06004087A (enExample)
PL (1) PL1687401T3 (enExample)
PT (1) PT1687401E (enExample)
WO (1) WO2005038002A2 (enExample)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL158418A0 (en) * 2001-04-19 2004-05-12 Scripps Research Inst In vivo incorporation of unnatural amino acids
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
BRPI0411475A (pt) 2003-06-18 2006-07-25 Scripps Research Inst adições de código genético de aminoácido reativo não natural
EP1666604B1 (en) * 2003-07-07 2008-02-13 The Scripps Research Institute Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
AU2005248392A1 (en) * 2004-05-25 2005-12-08 Irm, Llc Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
DE102004031258A1 (de) * 2004-06-29 2006-02-09 Jennissen, Herbert P., Prof. Dr. Proteinhybride mit polyhydroxyaromatischen Aminosäure-Epitopen
WO2006036779A2 (en) * 2004-09-22 2006-04-06 The Scripps Research Institute Site-specific labeling of proteins for nmr studies
EP2383340B1 (en) * 2004-10-27 2014-07-16 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US8367588B2 (en) * 2005-10-12 2013-02-05 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
US7776535B2 (en) 2006-02-06 2010-08-17 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
WO2007103490A2 (en) * 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
BRPI0709603A2 (pt) * 2006-03-16 2011-07-19 Scripps Research Inst expressão de proteìnas geneticamente programada contendo o aminoácido não natural fenilselenocisteìna
BRPI0711811A2 (pt) * 2006-05-23 2011-12-06 Scripps Research Inst aminoácidos de cumarina fluorescente geneticamente codificados
RU2009110152A (ru) * 2006-09-21 2010-10-27 Зе Скрипс Ресеч Инститьют (Us) Генетически закодированная формула селективно-сульфатных белков в эубактерии
KR20090094812A (ko) 2006-10-18 2009-09-08 더 스크립스 리서치 인스티튜트 포유동물 세포에서 비천연 아미노산의 단백질 내로의 유전적 도입
WO2008137452A1 (en) * 2007-05-04 2008-11-13 Kenergy Royalty Company, Llc Implantable high efficiency digital stimulation device
EP2217707B1 (en) 2007-10-25 2014-10-15 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
EP2220218A4 (en) * 2007-11-02 2010-12-08 Scripps Research Inst GENETICALLY ENCODED BORONATE AMINO ACID
US9249408B2 (en) * 2007-11-02 2016-02-02 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
CA2708760A1 (en) * 2007-12-11 2009-06-18 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
BRPI0906388A2 (pt) * 2008-02-08 2015-07-07 Scripps Research Inst Quebra de tolerância imunológica com um aminoácido não natural geneticamente codificado
EP2373224A1 (en) * 2008-12-09 2011-10-12 Wilson-Cook Medical Inc. Apparatus and methods for controlled release of tacking devices
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
WO2010089741A1 (en) * 2009-02-04 2010-08-12 Ben-Gurion University Of The Negev Research And Development Authority Systems and methods for bio-electricity production
US10233249B2 (en) 2013-03-19 2019-03-19 Beijing Shenogen Pharma Group Ltd. Antibodies and methods for treating estrogen receptor-associated diseases
US20150331624A1 (en) * 2014-05-19 2015-11-19 Kabushiki Kaisha Toshiba Host-controlled flash translation layer snapshot
KR101956042B1 (ko) * 2017-07-13 2019-03-08 서강대학교산학협력단 표적 단백질 내 l-디하이드록시페닐알라닌의 도입 방법
CN114008176A (zh) 2019-06-17 2022-02-01 默克专利股份有限公司 基于液晶的光阀

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5879905A (en) * 1996-08-29 1999-03-09 Massachusetts Institute Of Technology In vivo method of initiating protein synthesis with an amino acid other than methionine
IL158418A0 (en) * 2001-04-19 2004-05-12 Scripps Research Inst In vivo incorporation of unnatural amino acids
MXPA05003978A (es) 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
WO2004035743A2 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Site specific incorporation of keto amino acids into proteins
MXPA05006836A (es) 2002-12-22 2005-08-16 Scripps Research Inst Colecciones ordenadas de proteinas.
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
BRPI0411475A (pt) 2003-06-18 2006-07-25 Scripps Research Inst adições de código genético de aminoácido reativo não natural
US20060160175A1 (en) 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
EP1666604B1 (en) 2003-07-07 2008-02-13 The Scripps Research Institute Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Also Published As

Publication number Publication date
CN1942579A (zh) 2007-04-04
US20090298124A1 (en) 2009-12-03
ES2396712T3 (es) 2013-02-25
JP5657850B2 (ja) 2015-01-21
MXPA06004087A (es) 2006-06-27
WO2005038002A2 (en) 2005-04-28
IL201705A0 (en) 2011-08-01
AU2004282577A1 (en) 2005-04-28
DK1687401T3 (da) 2013-02-04
AU2004282577B2 (en) 2009-10-29
US8114654B2 (en) 2012-02-14
IL174947A0 (en) 2006-08-20
US20070009990A1 (en) 2007-01-11
US20120100570A1 (en) 2012-04-26
PL1687401T3 (pl) 2013-04-30
JP2007508034A (ja) 2007-04-05
US20110008782A1 (en) 2011-01-13
BRPI0415308A (pt) 2006-12-05
CN101532046A (zh) 2009-09-16
US7811801B2 (en) 2010-10-12
CN1942579B (zh) 2013-06-26
IL174947A (en) 2011-11-30
WO2005038002A3 (en) 2006-11-23
EP1687401A2 (en) 2006-08-09
US20050227318A1 (en) 2005-10-13
PT1687401E (pt) 2013-02-26
US7494796B2 (en) 2009-02-24
EP1687401A4 (en) 2007-08-01
US9783798B2 (en) 2017-10-10
IL206889A0 (en) 2010-12-30
EP1687401B1 (en) 2012-11-21
US8008041B2 (en) 2011-08-30

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EP1666604B1 (en) Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
EP1991680B1 (en) System for the expression of orthogonal translation components in eubacterial host cells
US7824893B2 (en) In vivo incorporation of alkynyl amino acids into proteins in eubacteria
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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20140821