CN1636051A

CN1636051A - 编码碳代谢和能量产生的蛋白质的基因

Info

Publication number: CN1636051A
Application number: CNA028245237A
Authority: CN
Inventors: O·策尔德尔; M·蓬佩尤斯; H·施罗德; B·克勒格尔; C·克洛普罗格; G·哈伯豪尔
Original assignee: BASF SE
Current assignee: BASF SE
Priority date: 2001-11-05
Filing date: 2002-10-31
Publication date: 2005-07-06
Also published as: EP1444351B1; US20070037261A1; WO2003040291A2; WO2003040291A3; DE10154270A1; DE50212612D1; ATE403730T1; US20070054382A1; AU2002346803A1; EP1944374A1; BR0213773A; US7355030B2; US7141664B2; US20070054383A1; US7355031B2; KR20050042248A; EP1444351A2; ZA200404423B; US7355029B2; US20050014233A1

Abstract

本发明涉及新的核酸分子，涉及其用于构建遗传改良的微生物的用途，还涉及借助于所述遗传改良的微生物制备精细化学品的方法，尤其是制备氨基酸的方法。

Description

编码碳代谢和能量产生的蛋白质的基因

发明背景

细胞中天然进行的代谢过程所产生的特定产物和副产物已用于工业的许多分支中，包括食品工业、动物饲料工业、化妆品工业及药学工业。这些分子合起来称为“精细化学品”，其包括有机酸(蛋白原的和非蛋白原的氨基酸)、核苷酸和核苷、脂类和脂肪酸、二醇、碳水化合物、芳香化合物、维生素和辅因子、以及酶。它们最好的产生是通过大规模培养已研发的在每一特定情况下可产生并分泌大量目标分子的细菌。一种对此目的尤其有用的生物体是谷氨酸棒状杆菌(Corynebacterium glutamicum)，其为革兰氏阳性的非致病菌。通过菌株选择，已开发了许多突变菌株，它们产生多种目标化合物。但是，为提高特定分子产量的菌株选择是一种费时且困难的方法。

发明简述

本发明提供了新的可用于谷氨酸棒状杆菌或相关菌种的鉴定或分类的核酸分子。谷氨酸棒状杆菌为革兰氏阳性需氧细菌，其广泛用在工业上用于大规模产生多种精细化学品，也用于降解碳氢化合物(如在原油泄漏物中)，以及用于氧化萜类化合物。本发明的核酸分子因此可用于鉴定能如通过发酵法用来产生精细化学品的微生物。虽然谷氨酸棒状杆菌自身为非致病的，但它与其它棒状杆菌如对人致病的重要病原菌白喉棒状杆菌(Corynebacterium diphtheriae)(白喉的病原菌)有亲缘关系。所以鉴定存在棒状杆菌的能力也具有显著的临床重要性，例如诊断应用。而且，所述核酸分子可作为对谷氨酸棒状杆菌或相关生物进行基因组作图的参考点。

这些新核酸分子编码的蛋白文中称为糖代谢和氧化磷酸化(SMP)蛋白。这些SMP蛋白可例如在谷氨酸棒状杆菌中于碳化合物如糖的代谢或通过如氧化磷酸化产生能量分子的过程中起作用。由于可得到用于谷氨酸棒状杆菌中的克隆载体(如在Sinskey等人，美国专利号4,649,119中所公开的)和用于遗传操作谷氨酸棒状杆菌和相关短杆菌种(如lactofermentum)的技术(Yoshihama等人，细菌学杂志(J.Bacteriol.)162：(1985)591-597；Katsumata等人，细菌学杂志(J.Bacteriol.)159：(1984)306-311；和Santamaria等人，普通微生物学杂志(J.Gen.Microbiol.)130：(1984)2237-2246)，本发明的核酸分子可用于该生物体的遗传操作，使其成为一种或多种精细化学品的较好和更有效的生产者。通过操作本发明的基因可直接或间接地导致精细化学品的生产率或生产效率提高。

通过许多机制修饰本发明的SMP蛋白可直接影响包含该经修饰蛋白的谷氨酸棒状杆菌中精细化学品的产量、生产率和/或生产效率。高能碳分子如糖的降解以及像NADH和FADH₂这样的化合物通过氧化磷酸化转化为具高能磷酸键的化合物导致许多化合物如丙酮酸、ATP、NADH和许多糖中间体自身即为所希望的精细化学品。而且，这些代谢途径所产生的能量分子(如ATP)以及还原型等同物(如NADH或NADPH)在细胞中用于驱动细胞中能量逆差的反应。此类能量逆差的反应包括精细化学品的许多生物合成途径。通过提高细胞利用特定糖的能力(例如，通过操纵细胞中参与将所述糖降解并转化为能量的基因)，可增加用于进行能量逆差的、但为所需要的代谢反应(如目标精细化学品的生物合成)的能量。

诱变本发明的一个或多个SMP蛋白也可产生活性改变的SMP蛋白，其中所述活性改变的SMP蛋白间接影响谷氨酸棒状杆菌中一种或多种目标精细化学品的生产率。例如，可通过提高对一种或多种糖的利用效率(以便增加将所述糖转化为可利用的能量分子)或通过增加将还原型等同物转化为可利用能量分子的效率(例如，通过提高氧化磷酸化效率或ATP合酶的活性)，从而增加这些高能化合物的量，其中所述高能化合物可用于细胞驱动通常为能量逆差的代谢过程。这些过程包括细胞壁的构建、转录、翻译以及细胞生长和细胞分裂所需的化合物(如核苷酸、氨基酸、维生素、脂类等)的生物合成(Lengeler等(1999)，原核生物学(Biology of Prokaryotes)，Thieme Verlag：Stuttgart，88-109页；913-918页；875-899页)。这些经修饰细胞生长和增殖的改善使得可在大规模培养物中增加所述细胞的成活力并且可提高它们的分裂速率，从而相对大量的细胞可在发酵培养物中存活。产量、生产率或生产效率可得以提高，这至少是由于存在大量在所有情况下均产生目标精细化学品的活细胞。在糖代谢中产生的许多降解产物也被细胞用作产生其它目标产物(如精细化学品)的前体或中间体。细胞代谢糖能力的提高增加了细胞可用于其它过程的所述降解产物的量。

本发明提供了这样的新核酸分子，其编码的蛋白文中称为SMP蛋白，所述蛋白例如可在谷氨酸棒状杆菌中于碳化合物如糖的代谢或通过如氧化磷酸化产生能量分子的过程中起作用。编码SMP蛋白的核酸分子文中称为SMP核酸分子。在一个优选的实施方案中，SMP蛋白参与将碳分子及其降解产物转化为细胞用于代谢过程的能量。此类蛋白的实例为被表1中所示基因编码的蛋白。

因此，本发明的一个方面涉及这样的分离的核酸分子(例如cDNAs)，其包含编码SMP蛋白或其生物学活性部分的核苷酸序列和适合作为检测或扩增编码SMP的核酸(例如DNA或mRNA)的引物或杂交探针的核酸片段。在尤其优选的实施方案中，该分离的核酸分子包含任何集合A所列出的核苷酸序列或其编码区或其互补序列。在其它优选的实施方案中，该分离的核酸分子编码集合B中列出的任何氨基酸序列。本发明优选的SMP蛋白同样优选地具有至少一种本文所描述的SMP活性。

集合A定义了下面的表1中描述的序列表的核酸序列以及相关位置的序列修饰。

集合B定义了下面的表1中描述的序列表的多肽序列以及相关位置的序列修饰。

在另一个实施方案中，分离的核酸分子长度至少为15个核苷酸并且在严紧条件下与含有集合A的核苷酸序列的核酸分子杂交。分离的核酸分子优选与天然存在的核酸分子对应。分离的核酸更优选编码天然存在的谷氨酸棒状杆菌SMP蛋白或其生物活性部分。

本发明的另一个方面涉及包含本发明核酸分子的载体(例如重组表达载体)，还涉及已导入所述载体的宿主细胞。在一个实施方案中，通过使用在合适的培养基中培养的宿主细胞制备了SMP蛋白。然后可从培养基或宿主细胞中分离该SMP蛋白。

本发明的另一个方面涉及已导入SMP基因的或其中SMP基因被修饰的经遗传修饰的微生物。在一个实施方案中，通过导入至少一种作为转基因的编码突变SMP序列的本发明核酸分子而修饰了所述微生物的基因组。在另一个实施方案中，通过用经修饰的SMP基因进行同源重组而修饰(例如功能性破坏)了所述微生物基因组中的内源性SMP基因。在一个优选的实施方案中，微生物属于棒状杆菌属或短杆菌(Brevibacterium)属，尤其优选谷氨酸棒状杆菌。在一个优选的实施方案中，该微生物也被用于制备目标化合物，如氨基酸、尤其是赖氨酸。

另一个优选的实施方案是具有多于一种的集合A中所描述核酸分子的宿主细胞。可用技术人员已知的多种方法制备这些宿主细胞。例如，可通过携带几种本发明核酸分子的载体转染这些细胞。然而，也可使用每次导入一种本发明核酸分子的载体从而同时或相继使用多种载体。从而可构建携带许多、高达数百的本发明核酸序列的宿主细胞。这种积累通常能够对宿主细胞精细化学品的生产率产生超加性效果。

本发明的另一方面涉及分离的SMP蛋白或其部分，例如其生物活性部分。在一个优选的实施方案中，分离的SMP蛋白或其部分可在谷氨酸棒状杆菌中于碳化合物如糖的代谢或通过如氧化磷酸化产生能量分子(如ATP)的过程中起作用。在另一个优选的实施方案中，分离的SMP蛋白或其部分与集合B的氨基酸序列足够同源，由此该蛋白或其部分仍然能够在谷氨酸棒状杆菌中于碳化合物如糖的代谢或通过如氧化磷酸化产生能量分子(如ATP)的过程中起作用。

此外，本发明涉及分离的SMP蛋白制品。在优选的实施方案中，该SMP蛋白含有集合B的氨基酸序列。在另一个优选的实施方案中，本发明涉及分离的全长蛋白，其和集合B的全长氨基酸序列(其由集合A中的可读框编码)充分同源。

可以将SMP多肽或其生物活性部分与非SMP多肽功能性地连接以产生融合蛋白。在优选的实施方案中，该融合蛋白具有不同于单独的SMP蛋白的活性。在其它优选的实施方案中，该融合蛋白可在谷氨酸棒状杆菌中于碳化合物如糖的代谢或通过如氧化磷酸化产生能量分子(如ATP)的过程中起作用。在尤其优选的实施方案中，所述融合蛋白向宿主细胞的整合调节细胞对目的化合物的生产率。

本发明的另一个方面涉及制备精细化学品的制备方法。该方法涉及含有载体的细胞的培养，这导致本发明SMP核酸分子的表达而产生精细化学品。在一个优选的实施方案中，该方法还包括得到含有此类载体的细胞的步骤，所述细胞用载体转染导致SMP核酸表达。在另一个优选的实施方案中，所述方法还包括从培养物中得到精细化学品的步骤。在一个优选的实施方案中，该细胞属于棒状杆菌属或短杆菌属。

本发明的另一个方面涉及调节来自微生物的分子生产率的方法。这些方法包括将细胞与调节SMP蛋白活性或SMP核酸表达的物质接触，使得和没有所述物质存在时相比细胞相关的活性受到调节。在一个优选的实施方案中，谷氨酸棒状杆菌细胞中一个或多个碳代谢途径或通过如氧化磷酸化产生能量分子(如ATP)的过程受到调节，使得该微生物的目标精细化学品的产量或生产率提高。调节SMP蛋白活性的物质刺激例如SMP蛋白的活性或SMP核酸的表达。刺激SMP蛋白活性或SMP核酸表达的物质的例子包括小分子、活性SMP蛋白和编码SMP蛋白并已导入细胞的核酸分子。抑制SMP活性或SMP表达的物质的例子包括小分子和反义SMP核酸分子。

本发明的另一个方面涉及调节细胞的目的化合物产量的方法，该方法包括向细胞中导入SMP野生型基因或SMP突变基因，所述基因仍位于分立的质粒上或者整合到宿主细胞的基因组中。向基因组的整合可随机发生或者通过同源重组发生，由此天然基因被整合的拷贝所代替，导致细胞的目标化合物的生产率被调节。在一个优选的实施方案中，所述产量增加了。在另一个优选的实施方案中，化学品是精细化学品，其在一个特别优选的实施方案中是氨基酸。在一个特别优选的实施方案中，该氨基酸是L-赖氨酸。

发明详述

本发明提供了在谷氨酸棒状杆菌中参与碳化合物如糖的代谢以及参与通过如氧化磷酸化过程产生能量分子(如ATP)的SMP核酸和SMP蛋白分子。本发明的分子可用于直接调节(例如，当过表达或优化糖酵解途径中的蛋白对经修饰的谷氨酸棒状杆菌的例如丙酮酸的产量、生产率和/或生产效率具有直接影响时)或通过导致目的化合物的产量、生产率和/或生产效率增加的间接作用(例如，当调节参与氧化磷酸化的蛋白导致进行必需的代谢过程和其它细胞功能如核酸和蛋白质生物合成以及转录/翻译可获得的能量发生变化时)调节微生物如谷氨酸棒状杆菌的精细化学品的生产率。本发明的诸方面进一步阐述如下。

I.精细化学品

术语“精细化学品”为本领域所认知，其包括已用于多种工业的由生物体产生的分子，其中所述工业例如但不限于药学工业、农业工业和化妆品工业。此类化合物包括有机酸如酒石酸、衣康酸和二氨基庚二酸、蛋白原的和非蛋白原的氨基酸、嘌呤和嘧啶碱基、核苷和核苷酸(如在Rehm等人编辑的生物技术(Biotechnology)第6卷中Kuninaka，A.(1996)核苷酸和相关化合物(Nucleotides and related compounds)，561-612页，VCH：Weinheim及其中所含参考文献中所述)、脂类、饱和和不饱和脂肪酸(如花生四烯酸)、二醇(如丙二醇和丁二醇)、碳水化合物(如透明质酸和海藻糖)、芳香化合物(如芳香胺、香草醛和靛蓝)、维生素和辅因子(如在Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)，A27卷，“维生素”(“Vitamins”)，443-613页(1996)VCH：Weinheim及其中的参考文献；以及Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病”论文集(“Nutrition，Lipids，Health，and Disease”Proceedings ofthe UNESCO/Confederation of Scientific and Technological Associationsin Malaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press，(1995))、酶以及在Gutcho(1983)通过发酵的化学品(Chemicals by Fermentation)，Noyes DataCorporation，ISBN：0818805086和其中的参考文献中所述的所有其它化学品。这些精细化学品中的某些化学品的代谢和用途进一步阐述如下。

A.氨基酸代谢和用途

氨基酸包含所有蛋白质的基本结构单元，并且为所有生物体的正常细胞功能所必需。术语“氨基酸”为本领域所认知。蛋白原的氨基酸有20种，为蛋白质的结构单元，在蛋白质中它们通过肽键连接，非蛋白原的氨基酸(已知成百种非蛋白原的氨基酸)在蛋白质中通常不存在(参见Ulmann工业化学百科全书(Ulmann工业化学百科全书(Ulmann’s Encyclopediaof Industrial Chemistry))，A2卷，57-97页，VCH：Weinheim(1985))。氨基酸可为D-或L-构型，尽管在天然存在的蛋白质中发现的唯一类型通常为L-氨基酸。已充分说明了20种蛋白原氨基酸中每一种在原核和真核细胞中的生物合成和降解途径(参见例如，Stryer，L.生物化学(Biochemistry)，第三版，578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸)，这样称呼是因为由于它们生物合成的复杂性，必须从食物中摄取它们，它们被简单的生物合成途径转化为其余11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)。高等动物能够合成这些氨基酸中的一部分氨基酸，但必需氨基酸必须从食物中摄取，以进行正常的蛋白质合成。

除了它们在蛋白质生物合成中的功能外，这些氨基酸本身还是令人感兴趣的化学品，并且许多已发现在食品、动物饲料、化学品、化妆品、农业和药学工业中具有多种应用。赖氨酸不仅对人的营养而且对单胃动物如禽和猪都是一种重要的氨基酸。谷氨酸盐是最常用的调味添加剂(谷氨酸单钠盐，MSG)，并且广泛用于食品工业，天冬氨酸、苯丙氨酸、甘氨酸和半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸均用于药学工业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸和丙氨酸用于药学工业和化妆品工业。苏氨酸、色氨酸和D/L-甲硫氨酸是广泛使用的动物饲料添加剂。(Rehm等人(编辑)，生物技术(Biotechnology)，第6卷，第14a章中的Leuchtenberger，W.(1996)Amino aids-technicalproduction and use，466-502页，VCH：Weinheim)。还发现这些氨基酸可用作前体，用于合成合成的氨基酸和蛋白质，如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟色氨以及在其他Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)，A2卷，57-97页，VCH：Weinheim，1985中所述的物质。

在可产生它们的生物体如细菌中已充分描述了这些天然氨基酸的生物合成(细菌氨基酸生物合成及其调节的综述，见Umbarger，H.E.(1978)Ann.Rev.Biochem.47：533-606)。谷氨酸通过还原胺化α-酮戊二酸(柠檬酸循环中的中间体)而合成。谷氨酰胺、脯氨酸和精氨酸接下来由谷氨酸产生。丝氨酸的生物合成为三步，由3-磷酸甘油(糖酵解中间体)开始，在氧化、氨基转移和水解步骤后产生该氨基酸。半胱氨酸和甘氨酸两者均产生自丝氨酸；在丝氨酸转羟甲基酶催化的反应中前者通过高半胱氨酸与丝氨酸缩合，后者通过侧链β-碳原子转移至四氢叶酸。苯丙氨酸和酪氨酸在9步的生物合成途径中合成自糖分解和磷酸戊糖途径的前体赤藓糖4-磷酸和磷酸烯醇丙酮酸，它们的不同仅在预苯酸合成后的最后两步。色氨酸也从这两种起始分子产生，但其合成为11步的途径。酪氨酸也可在苯丙氨酸羟化酶催化的反应中由苯丙氨酸合成。丙氨酸、缬氨酸和亮氨酸均为丙酮酸盐(糖酵解的终产物)的生物合成产物。天冬氨酸形成自草酰乙酸(柠檬酸循环的中间体)。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸均通过转化天冬氨酸而形成。异亮氨酸从苏氨酸形成。组氨酸在一个复杂的9步途径中从一种活化糖——5-磷酸核糖-1-焦磷酸形成。

为细胞蛋白质合成所需的过量氨基酸不能被贮藏，并被降解以提供用于细胞主要代谢途径的中间体(综述参见Stryer，L.生物化学(Biochemistry)第三版，21章，“氨基酸降解和尿素循环”495-516页(1988))。虽然细胞可将不想要的氨基酸转化为有用的代谢中间体，从所需用于合成氨基酸的能量、前体分子和酶来说，氨基酸的产生是昂贵的。因此对于氨基酸的生物合成受到反馈抑制调节并不让人吃惊，其中特定氨基酸的存在起到减缓或完全终止其自身产生的作用(关于氨基酸生物合成途径中的反馈机制的综述参见Stryer，L.生物化学(Biochemistry)第三版，24章：”氨基酸和血红素的生物合成”575-600页(1988))。因此，任一特定氨基酸的输出受到细胞中存在的该氨基酸量的限制。

B.维生素、辅因子和营养成分的代谢和用途

维生素、辅因子和营养成分包含高等动物不能合成且必须摄取的另一分子，虽然它们易于被其它生物体如细菌合成。这些分子自身为生物活性物质或为在多种代谢途径中作为电子载体或中间体的生物活性物质的前体。除了它们的营养价值外，这些化合物作为着色剂、抗氧化剂和催化剂或其它操作辅助剂也具有重要的工业价值(这些化合物的结构、活性和工业应用的概述参见例如，Ullman工业化学百科全书(Ullman’s Encyclopediaof Industrial Chemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996)。术语”维生素”为本领域认知，包括生物体行使正常功能所需的营养素，且该生物体自身不能合成它们。维生素组和包含辅因子和营养性化合物。术语“辅因子”包括进行正常酶促活性所需的非蛋白原化合物。此类化合物可为有机的或无机的，本发明的辅因子优选有机的。术语“营养成分”包括促进植物和动物尤其是人的健康食物添加剂。此类分子的实例为维生素、抗氧化剂和某些脂类(如多不饱和脂肪酸)。

已大量描述了这些分子在可产生它们的生物体如细菌中的生物合成(Ullman工业化学百科全书(Ullman’s Encyclopedia of IndustrialChemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(BiochemicalPathways：An Atlas of Biochemistry and Molecular Biology)，John Wiley& Sons；Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病”论文集(“Nutrition，Lipids，Health，and Disease”Proceedings of theUNESCO/Confederation of Scientific and Technological Associations inMalaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press：Champaign，IL X，374 S)。

硫胺素(维生素B₁)由嘧啶和噻唑部分通过化学偶联产生。核黄素(维生素B₂)由鸟苷-5’-三磷酸(GTP)和核糖-5’-磷酸合成。核黄素接下来用于合成黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。合起来称为‘维生素B₆’的化合物类(例如，吡哆素、吡哆胺、5’-磷酸吡哆醛和商业上使用的维生素B₆盐酸盐)均为共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸盐(泛酸(R)-(+)-N-(2，4-二羟基-3，3-二甲基-1-氧代丁基)-β-丙氨酸)可通过化学合成或发酵产生。泛酸生物合成的最后步骤由ATP-驱动的β-丙氨酸和泛解酸的缩合组成。负责转化为泛解酸、β-丙氨酸和泛酸缩合生物合成步骤的酶是已知的。泛酸盐的代谢活性形式为辅酶A，其生物合成由5步酶促步骤进行。泛酸盐、5’-磷酸吡哆醛、半胱氨酸和ATP为辅酶A的前体。这些酶不仅催化泛酸盐的形成，而且催化(R)-泛解酸、(R)-pantolacton、(R)-泛醇(维生素B₅原)、泛酰巯基乙胺(及其衍生物)和辅酶A的产生。

已详细研究了微生物中生物素从前体分子庚二酰辅酶A的生物合成，并鉴定了几种所涉及的基因。发现许多相应的蛋白质也参与Fe-簇合成，属于nifS蛋白质类。硫辛酸衍生自辛酸，并在能量代谢中作为辅酶，此时其成为丙酮酸脱氢酶复合体和α-酮戊二酸脱氢酶复合体的一部分。叶酸盐为一组这样的物质，其均为叶酸衍生物，来自于L-谷氨酸、对氨基苯甲酸和6-甲基蝶呤。已详细研究了某些微生物中始于生物转化代谢中间体鸟苷-5’-三磷酸(GTP)、L-谷氨酸和对氨基苯甲酸的叶酸及其衍生物的生物合成。

类可啉(如钴胺素且尤其是维生素B₁₂)和卟啉属于一组四吡咯环体系的化学品。维生素B₁₂的生物合成太复杂，目前尚未彻底解释清楚，但许多涉及的酶和物质已知。烟酸(烟酸盐)和烟酰胺为吡啶衍生物，也称为‘烟酸’。烟酸为重要的辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)和它们的还原形式的前体。

这些化合物的大规模产生很大程度上依赖于无细胞的化学合成，虽然这些化学品中的一些如核黄素、维生素B₆、泛酸盐和生物素也已通过微生物的大规模培养物产生。只有维生素B₁₂是仅通过发酵产生的，因为其合成的复杂性。体外方法需要耗费材料和时间，通常以高的代价耗费。

C.嘌呤、嘧啶、核苷和核苷酸的代谢和用途

嘌呤和嘧啶代谢基因及它们相应的蛋白质为治疗肿瘤和病毒感染的重要靶标。术语“嘌呤”或“嘧啶”包括构成核酸、辅酶和核苷酸的含氮碱基。术语“核苷酸”包括核酸分子的基本结构单元，其由含氮碱基、戊糖(对RNA而言，该糖为核糖，对DNA而言，该糖为D-脱氧核糖)和磷酸组成。术语“核苷”包括作为核苷酸前体的分子，但其不含核苷酸所具有的磷酸单元。通过抑制这些分子的生物合成，或抑制它们形成核酸分子的代谢，可抑制RNA和DNA合成；通过以靶向癌细胞的方式抑制这种活性，可抑制肿瘤细胞分裂和复制的能力。

此外，有不形成核酸分子但作为能量贮存体(即AMP)或作为辅酶(即FAD和NAD)的核苷酸。

一些出版物已描述了这些化学品通过影响嘌呤和/或嘧啶代谢对这些医学适应症的用途(例如Christopherson，R.I.和Lyons，S.D.(1990)“作为化学治疗剂的嘧啶和嘌呤从头生物合成的强力抑制剂”医学研究述评(Med.Res.Reviews)10：505-548)。对参与嘌呤和嘧啶代谢的酶的研究集中在研发可用作如免疫抑制剂或抗增生剂的新药上(Smith，J.L.，(1995)“核苷酸合成中的酶”结构生物学最新观点(Curr.Opin.Struct.Biol.)5：752-757；(1995)Biochem Soc.Transact.23：877-902)。但是，嘌呤和嘧啶碱基、核苷和核苷酸具有其它应用：用作一些精细化学品(例如，硫胺素、S-腺苷-甲硫氨酸、叶酸盐或核黄素)生物合成的中间体，作为细胞的能量载体(例如，ATP或GTP)，和本身作为化学品，通常作为增味剂(例如，IMP或GMP)或用于一些医药用途(参见例如，Kuninaka，A.(1996)生物技术中的核苷酸和相关化合物(Nucleotides and Related Compounds inBiotechnology)第6卷，Rehm等人编辑.VCH：Weinheim，561-612页)。同样，参与嘌呤、嘧啶、核苷或核苷酸代谢的酶越来越多地用作靶，研发用于作物保护的抗这些酶的化学品包括杀真菌剂、除草剂和杀虫剂。

已阐释了这些化合物在细菌中的代谢(综述参见例如，核酸研究和分子生物学进展(Progress in Nucleic Acid Research and Molecular Biology)，第42卷，Academic Press中的Zalkin，H.和Dixon，J.E.(1992)“嘌呤核苷酸的从头生物合成”，259-287页；和生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，Wiley：纽约一书中的Michal，G.(1999)“核苷酸和核苷”，第8章)。嘌呤代谢已是深入研究的主题，其对细胞的正常功能是必需的。高等动物中受损的嘌呤代谢可引起严重疾病，如痛风。嘌呤核苷酸从核糖-5-磷酸开始，经过一系列步骤，通过中间体化合物次黄苷-5’-磷酸(IMP)，产生鸟苷-5’-单磷酸(GMP)或腺苷-5’-单磷酸(AMP)，由此易于形成用作核苷酸的三磷酸形式。这些化合物也用作能量贮存体，这样它们的降解可提供细胞内用于许多不同生物化学过程的能量。嘧啶的生物合成由核糖-5-磷酸形成尿苷-5’-单磷酸(UMP)而进行。UMP接下来转化为胞苷-5’-三磷酸(CTP)。所有这些核苷酸的脱氧形式通过一步还原步骤从核苷酸的二磷酸核糖形式产生为核苷酸的二磷酸脱氧核糖形式。通过磷酸化后，这些分子可参与DNA合成。

D.海藻糖的代谢和用途

海藻糖由两个葡萄糖分子通过α，α-1，1连接组成。它通常作为甜味剂用于食品工业，为干的或冷冻食品和饮料中的添加剂。但它也用于药学、化妆品和生物技术工业(参见例如，Nishimoto等人(1998)美国专利号5,759,610；Singer，M.A.和Lindquist，S.生物技术趋势(Trends Biotech.)16(1998)：460-467；Paiva，C.L.A.和Panek，A.D.Biotech.Ann.Rev.2(1996)：293-314；和Shiosaka，M.J.Japan 172：(1997)97-102)。海藻糖通过酶从许多微生物产生，并天然释放入环境介质中，从中可用本领域公知的方法收集海藻糖。

II.糖和碳分子的用途和氧化磷酸化

碳对所有有机物质的形成均是至关重要的物质，因此必须具有营养地摄入，这不仅用于谷氨酸棒状杆菌的生长和分裂，而且用于该微生物超量产生精细化学品。糖类如单糖、二糖或多糖为特别好的碳源，标准的生长培养基因此通常包含一种或多种的葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉和纤维素(Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)(1987)，A9卷，“酶”(“Enzymes”)，VCH：Weinheim)。作为备选，可在培养基中使用较复杂的糖化合物如糖蜜或糖精制中的副产物。除了糖外，也可用其它的化合物作为备选碳源，例如醇类(如甲醇或乙醇)、烃类、糖醇、脂肪酸和有机酸(如醋酸或乳酸)。有关培养物中的碳源及微生物对其利用的概览见Ullmann工业化学百科全书(Ullmann’s Encyclopedia ofIndustrial Chemistry)(1987)，A9卷，“酶”(“Enzymes”)，VCH：Weinheim；Stoppok，E.和Buchholz，K.(1996)，“发酵应用中基于糖的原材料”，见生物技术(Biotechnology)(Rehm，H.J.等编辑)第6卷，VCH：Weinheim，S.5-29；Rehm，H.J.(1980)，Industrielle Mikrobiologie，Springer：Berlin；Batholomew，W.H.和Reiman，H.B.(1979)，发酵过程的经济学，见Peppler，H.J.和Perlman，D.编辑的微生物技术(MicrobialTechnology)，第二版，2卷，18章，Academic Press：New York；以及Kockova-Kratachvilowa，A.(1981)，1卷，1章，Verlag Chemie：Weinheim。

所述的高能碳分子被摄入后，必须经过加工，这样它们可通过糖的主代谢途径之一降解。这些途径直接导致有用的降解产物，如5-磷酸核糖和烯醇丙酮酸磷酸，其中所述烯醇丙酮酸磷酸接下来可转化为丙酮酸。在细菌中三个最重要的糖代谢途径为Embden-Meyerhoff-Parnas(EMP)途径(也称为糖酵解或果糖二磷酸途径)、己糖单磷酸(HMP)途径(也称为戊糖二级代谢途径或磷酸戊糖途径)和Entner-Doudoroff(ED)途径(综述见Michal，G.(1999)，生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，Wiley：New York，以及Stryer，L.(1988)，生物化学(Biochemistry)，13-19章，Freeman：New York，和其中的参考文献)。

EMP途径将己糖分子转化为丙酮酸，该过程产生2个ATP分子和2个NADH分子。从葡糖-1-磷酸(其从培养基中直接摄入，或备选地可从糖原、淀粉或纤维素产生)开始，该葡萄糖分子异构化为果糖-6-磷酸、磷酸化并裂解为2个3-磷酸甘油醛分子，每个3-磷酸甘油醛分子具有3个碳原子。经过脱氢作用、磷酸化作用和接下来重排产生丙酮酸。

HMP途径将葡萄糖转化为还原型等同物如NADH，并产生戊糖和四糖化合物，它们在许多其它代谢途径中作为中间体和前体被需要。在HMP途径中，葡糖-6-磷酸通过两次连续的脱氢酶反应(也释放两个NADPH分子)和一步羧基化转化为5-磷酸核酮糖。5-磷酸核酮糖也可转化为5-磷酸木酮糖和5-磷酸核糖；在一系列的生物化学步骤中，前者可成为能进入EMP途径的葡糖-6-磷酸，而后者通常用作细胞中其它生物合成途径的中间体。

ED途径的起始化合物为葡萄糖或葡糖酸，其随后磷酸化并脱水以产生2-脱氢-3-脱氧-6-磷酸葡糖酸。葡糖醛酸和半乳糖醛酸也可通过较复杂的生物化学途径转化为2-脱氢-3-脱氧-6-磷酸葡糖酸。该产物分子然后裂解为3-磷酸甘油醛和丙酮酸；3-磷酸甘油醛本身可转化为丙酮酸。

EMP和HMP途径有许多相同的特性，其中包括相同的中间体和酶。EMP途径提供最大量的ATP，但既不产生5-磷酸核糖(一种重要的前体，例如用于核酸的生物合成)，也不产生赤藓糖4-磷酸(其对氨基酸生物合成是重要的)。利用葡萄糖时仅使用EMP途径的微生物因此不能在以葡萄糖作为唯一碳源的简单培养基上生长。它们被称为在合成培养基上不易生长的生物，它们的生长需要提供在酵母提取物中存在的复合有机化合物。

相反，HMP途径产生为核酸和氨基酸生物合成所需的全部前体，但仅提供EMP途径所提供的ATP能量的一半。所述HMP途径也产生NADPH，后者可用于生物合成途径中的氧化还原反应。但是，所述HMP途径不直接产生丙酮酸，结果所述微生物必须也拥有部分的EMP途径。因此许多微生物在进化过程中以拥有这两个途径的方式发育并不令人吃惊。

由所述途径产生的丙酮酸分子通过Krebs循环(也称为柠檬酸循环或三羧酸循环(TCA循环))易于转化为能量。在该过程中，丙酮酸首先脱羧，产生一分子的NADH、一分子乙酰辅酶A和一分子CO₂。乙酰辅酶A中的乙酰基随后与包含4个碳原子的草酰乙酸反应，产生柠檬酸，所述柠檬酸为具有6个碳原子的有机酸。最后，草酰乙酸再生，并可重新用作乙酰基受体，由此完成循环。TCA循环中中间体氧化所释放的电子转移至NAD⁺，产生NADH。

在呼吸作用进行期间，电子从NADH转移至分子氧或其它末端电子受体。该过程由呼吸链催化，其中所述呼吸链为一电子运送体系，其包含整合膜蛋白和膜相联蛋白。该体系有两个基本任务：首先，接受来自电子供体的电子并将它们转移至电子受体，其次，它通过合成ATP保存电子转移过程中释放的部分能量。已知几种类型的氧化/还原酶和电子转运蛋白参与所述过程，包括NADH脱氢酶、含黄素的电子转移分子、铁硫蛋白和细胞色素。NADH脱氢酶位于质膜的胞质表面，将氢原子从NADH转移至黄素蛋白，黄素蛋白接下来接受来自NADH的电子。黄素蛋白为一组电子转移分子，其具有推定的黄素基团，当接受和转移电子时，该基团或被还原或被氧化。已知有三种黄素参与这些反应：核黄素、黄素腺嘌呤二核苷酸(FAD)和黄素单核苷酸(FMN)。铁硫蛋白包含多个不与血红素基团结合但可参与脱水和再水化反应的铁和硫原子。琥珀酸脱氢酶和顺乌头酸酶为铁硫蛋白的实例；它们的铁硫复合体作为完整电子转运链的部分可接受和转移电子。细胞色素为包含铁卟啉环(血红素)的蛋白。存在许多不同类的细胞色素，不同之处在于它们的还原电势。功能上而言，这些细胞色素形成了在其中电子可转移至具有更大正还原电势的其它细胞色素的路径。另一类非蛋白的电子转移分子已知为：脂溶性醌类(如辅酶Q)。这些分子也起着氢受体和电子供体的作用。

呼吸链的作用产生了跨细胞膜的质子梯度，从而导致了质子动力。细胞利用该动力通过跨膜的ATP合酶合成ATP。所述ATP合酶为多蛋白复合体，其中H⁺分子跨膜的转运引起其胞内亚单位的物理运动并同时引起ADP磷酸化形成ATP(综述见Fillingame，R.H.和Divall，S.(1999)NovartisFound Symp.221：218-229，229-234)。

不为己糖的碳物质同样可作为细胞的碳源和能源。这些物质可首先在葡糖异生途径中转化为己糖，然后降解产生能量，在所述的葡糖异生途径中细胞首先合成葡萄糖。该反应的起始物质为烯醇丙酮酸磷酸(PEP)，其为糖酵解的最重要中间体之一。PEP除了来自糖外，也可从其他物质如乙酸形成，或通过草酰乙酸(其本身为TCA循环的中间体)的脱羧反应形成。葡糖6-磷酸可通过反向糖酵解形成(使用不同于原来糖酵解的酶级联)。将丙酮酸转化为葡萄糖需要使用6个高能磷酸键，而在葡萄糖转化为丙酮酸期间糖酵解只产生2个ATP。葡萄糖的完全氧化(糖酵解、将丙酮酸转化为乙酰辅酶A、柠檬酸循环和氧化磷酸化)产生36-38个ATP，这样在葡糖异生过程中高能磷酸键的净损失不会超过通过氧化葡萄糖产生的所述高能分子。

III.本发明的组成要件和方法

本发明至少部分是基于本文中称作SMP核酸和SMP蛋白分子的新分子的检测，在谷氨酸棒状杆菌中这些分子参与将糖转化为有用的降解产物和能量(如能量)或参与其它过程如氧化磷酸化产生有用的高能分子(如ATP)。在一个实施方案中，SMP分子在谷氨酸棒状杆菌中参与碳化合物如糖的代谢或通过如氧化磷酸化过程产生能量分子(如ATP)。在一个优选的实施方案中，本发明SMP分子的活性对谷氨酸棒状杆菌中碳代谢和能量产生的作用影响所述生物体目标精细化学品的生产率。在一个尤其优选的实施方案中，本发明SMP分子活性的调节是以在谷氨酸棒状杆菌中本发明SMP蛋白参与的代谢和能量途径被调节的方式进行的，使得本发明SMP蛋白的产量、生产率和/或生产效率被调节，由此直接或间接调节谷氨酸棒状杆菌中目标精细化学品的产量、生产率或生产效率。

术语“SMP蛋白”或“SMP多肽”包含可在谷氨酸棒状杆菌中于碳化合物如糖的代谢或通过如氧化磷酸化产生能量分子(如ATP)的过程中起作用的蛋白。SMP蛋白的例子包括表1和集合A中所列出的SMP基因编码的那些蛋白。术语“SMP基因”和“SMP核酸序列”包括编码SMP蛋白的核酸序列，其含有一个编码区和相应的非翻译5’和3’序列区。SMP基因的例子在表1中列出。术语“生产率”为本领域已知并且包括发酵产物的浓度(例如在预定的时间段和预定的发酵体积产生的目标精细化学品(例如kg产物/h/l))的浓度。术语“生产效率”包括得到特定产物量所需时间(例如，细胞达到精细化学品的特定生产率所需时间)。术语“产量”或“产物/碳产量”为本领域已知并且包括将碳源转化成产物(即精细化学品)的效率。这例如通常表达为kg产物/kg碳源。增加化合物的产量或生产率为在预定期间内及特定的培养体积中增加了所得分子的量或该化合物适宜得到的分子的量。术语“生物合成”和“生物合成途径”为本领域已知并且包括细胞在例如多步过程或高度调节的过程中从中间物合成化合物，优选有机化合物。术语“降解”和“降解途径”为本领域已知并且包括细胞在例如多步过程或高度调节的过程中将化合物，优选有机化合物裂解成降解产物(用更通俗的术语：更小或更简单的分子)。术语“降解产物”为本领域已知并且包括化合物的降解产物，这些降解产物本身可为合适的前体(起始点)或中间体，为细胞生物合成其他化合物所需。术语“代谢”为本领域已知并且包括生物体中发生的全部生物化学反应。特定化合物的代谢(例如氨基酸如甘氨酸的代谢)因此包括细胞中该化合物的所有生物合成、修饰和降解途径。

在另一个实施方案中，微生物如谷氨酸棒状杆菌中本发明的SMP分子能够调节目标分子例如精细化学品的生产率。通过一些机制修饰本发明的SMP蛋白可直接影响包含所述经修饰蛋白的谷氨酸棒状杆菌菌株中精细化学品的产量、生产率和/或生产效率。高能碳分子如糖的降解以及化合物如NADH和FADH₂通过氧化磷酸化转化为更有用的化合物导致许多化合物如丙酮酸、ATP、NADH和许多糖中间体自身即为所希望的精细化学品。而且，这些代谢途径所产生的能量分子(如ATP)以及还原型等同物(如NADH或NADPH)在细胞中用于驱动能量逆差的反应。此类能量逆差的反应包括精细化学品的许多生物合成途径。通过提高细胞利用特定糖的能力(例如，通过操纵细胞中参与所述糖降解和转化的基因)，可增加用于进行能量逆差的、但为所需要的代谢反应(如目标精细化学品的生物合成)的能量。

诱变本发明的一个或多个SMP蛋白也可产生活性改变的SMP蛋白，其中所述活性改变的SMP蛋白间接影响谷氨酸棒状杆菌中一种或多种目标精细化学品的生产率。例如，可通过提高对一种或多种糖的利用效率(以便增加将所述糖转化为可利用的能量分子)或通过增加将还原型等同物转化为可利用能量分子的效率(例如，通过提高氧化磷酸化效率或ATP合酶的活性)，从而增加这些高能化合物的量，其中所述高能化合物可用于细胞驱动通常为能量逆差的代谢过程。这些过程包括细胞壁的构建、转录、翻译以及细胞生长和细胞分裂所需的化合物(如核苷酸、氨基酸、维生素、脂类等)的生物合成(Lengeler等(1999)，原核生物学(Biology of Prokaryotes)，Thieme Verlag：Stuttgart，88-109页；913-918页；875-899页)。这些经修饰细胞生长和增殖的改善使得可在大规模培养物中增加所述细胞的成活力并且可提高它们的分裂速率，从而相对大量的细胞可在发酵培养物中存活。产量、生产率或生产效率可得以提高，这至少是由于存在大量在所有情况下均产生目标精细化学品的活细胞。在糖代谢中产生的许多降解产物也被细胞用作产生其它目标产物(如精细化学品)的前体或中间体。例如，许多氨基酸自糖酵解或TCA循环通常产生的化合物直接合成(例如，丝氨酸是从糖酵解中间体3-磷酸甘油合成的)。由此，通过提高将糖转化为有用的能量分子的效率，可增加有用的降解产物的量。

制备本发明核酸序列的一个适宜的起点是谷氨酸棒状杆菌菌株的基因组，可以从美国典型培养物保藏中心得到该菌株(名称是ATCC 13032)。

用常规方法，通过表1所示的修饰可以从这些核酸序列制备本发明的核酸序列。

本发明的SMP蛋白或其生物学活性部分或片段可在谷氨酸棒状杆菌中参与碳化合物如糖的代谢或通过如氧化磷酸化过程产生能量分子(如ATP)或可以具有表1列出的一种或多种活性。

下面的分段详细描述本发明的各方面：

A.分离的核酸分子

本发明的一个方面涉及编码SMP分子或其生物活性部分的分离核酸分子及核酸分子片段，其中所述核酸分子片段足以用作杂交探针或引物，用来鉴定或扩增编码SMP的核酸(例如SMP DNA)。如此处所用，术语“核酸分子”是指包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)和用核苷酸类似物产生的DNA或RNA类似物。该术语也包括位于基因编码区3’和5’端的非翻译区：基因编码区5’端上游的至少约100个核苷酸序列和基因编码区3’端下游的至少约20个核苷酸序列。核酸分子可为单链或双链，但优选地为双链DNA。“分离的”核酸分子为从其它核酸分子分离的核酸分子，其存在于核酸的天然源中。优选地，“分离的”核酸不具有该核酸所来自的生物体基因组DNA中天然存在于该核酸侧翼的序列(即位于该核酸5’和3’端的序列)。例如，在不同实施方案中，该分离的SMP核酸分子可包含少于约5kb，4kb，3kb，2kb，1kb，0.5kb或0.1kb的该核酸所来自的细胞(如谷氨酸棒状杆菌细胞)基因组DNA中天然存在于该核酸分子侧翼的核苷酸序列。而且，“分离的”核酸分子如cDNA分子当通过重组技术产生时可基本上不含有其它细胞物质或培养基，当通过化学合成产生时，可基本上不含有化学前体或其它化学品。

本发明的核酸分子，如具有的核苷酸序列为集合A的核酸分子或其部分可用标准的分子生物学技术和本文提供的序列信息制备。例如，使用集合A全部或部分序列作为杂交探针和标准的杂交技术(例如，如在Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：A Laboratory Manual).第二版，冷泉港实验室，冷泉港实验室出版社，冷泉港，纽约，1989中所述)，可自谷氨酸棒状杆菌文库分离谷氨酸棒状杆菌SMP cDNA。而且，包含集合A的全部序列或其部分的核酸分子可使用根据该序列设计的寡核苷酸引物，应用聚合酶链反应分离(例如，包含集合A的全部或部分序列的核酸分子可使用根据该同样序列设计的寡核苷酸引物，应用聚合酶链反应分离)。例如，可从正常的内皮细胞分离mRNA(例如，通过Chirgwin等人(1979)生物化学(Biochemistry)18：5294-5299的硫氰酸胍提取法)以及用反转录酶(例如，莫洛尼鼠类白血病毒反转录酶，可从Gibco/BRL，Bethesda，MD获得；或AMV反转录酶，可从Seikagaku America，Inc.，St.Petersburg，FL获得)可制备cDNA。用于聚合酶链反应扩增的合成寡核苷酸引物可根据集合A所示的核苷酸序列之一设计。本发明的核酸可用cDNA或另外用基因组DNA作为模板、使用合适的寡核苷酸引物按照标准的PCR扩增技术来扩增。这样扩增的核酸可克隆入合适的载体并通过DNA序列分析鉴定。对应于SMP核苷酸序列的寡核苷酸可通过标准的合成技术制备，例如用DNA自动合成仪制备。

在一个优选的实施方案中，本发明的分离核酸分子包含集合A中所示的核苷酸序列之一。

在另一个优选的实施方案中，本发明的分离的核酸分子含有与任一集合A中所示核苷酸序列或其部分互补的核酸分子，所述核酸分子与集合A中所示任一核苷酸序列足够互补使其与集合A中所示任一序列杂交而得到稳定双链体。

在一个实施方案中，本发明的核酸分子编码这样的蛋白质或其部分，其包括的氨基酸序列充分同源于集合B的一个氨基酸序列，以至于该蛋白质或其部分仍然能够在谷氨酸棒状杆菌中起作用，其参与碳化合物如糖的代谢或通过如氧化磷酸化过程产生能量分子(如ATP)。此处所用术语“充分同源”是指蛋白质或其部分的氨基酸序列具有最小数的与集合B中氨基酸序列相同或等同的氨基酸残基(例如，氨基酸残基具有与集合B中序列之一的氨基酸残基相似的侧链)，以至于该蛋白质或其部分能够在谷氨酸棒状杆菌中起作用，其参与碳化合物如糖的代谢或通过如氧化磷酸化过程产生能量分子(如ATP)。此类糖代谢途径或能量产生系统中的蛋白质成员如此处所述，可参与一种或多种精细化学品的产生和分泌。此类活性的实例也在此进行了描述。因此，“SMP蛋白的功能”涉及直接或间接地对一种或多种精细化学品的产量、生产率和/或生产效率起作用。SMP蛋白活性的实例在表1中给出。

本发明SMP核酸分子编码的蛋白部分优选地是任何SMP蛋白的具生物学活性的部分。文中所用术语“SMP蛋白的生物学活性部分”包含SMP蛋白的一部分，例如结构域/基序，其在谷氨酸棒状杆菌中参与碳化合物如糖的代谢或参与能量产生途径或者具有表1中所示活性。为了确定SMP蛋白或者其生物学活性部分能否在谷氨酸棒状杆菌中参与碳化合物的转运和代谢或高能分子的产生，可以进行酶活性分析。这些分析方法在实施例部分的实施例8中详细描述，技术人员熟知这些方法。

除了群体中存在的SMP序列的天然变体外，技术人员还理解可通过突变向集合A的核苷酸序列中导入变化，由此导致所编码的SMP蛋白的氨基酸序列改变，但SMP蛋白的功能不变。例如，可在集合A的核苷酸序列中进行在“非必需”氨基酸残基处导致氨基酸替换的核苷酸替换。可以修饰任一SMP蛋白(集合B)的野生型序列中的“非必需”氨基酸残基而不改变所述SMP蛋白的活性，但“必需”氨基酸残基为SMP蛋白活性所需。然而，其它氨基酸残基(例如，在具有SMP活性的结构域中不保守或仅为半保守的那些氨基酸残基)对活性可为非必需的，并因此可被改变，而此类氨基酸残基的改变不改变SMP活性。

编码同源于集合B的蛋白质序列的SMP蛋白的分离核酸分子可通过将一个或多个核苷酸替换、添加或删除导入集合A的核苷酸序列而产生，这样将一个或多个氨基酸的替换、添加或删除导入所编码的蛋白质。可通过标准技术将突变导入集合A的核苷酸序列之一，如定点诱变和PCR-介导的诱变。优选地，在一处或多处预测的非必需氨基酸残基处进行保守氨基酸替换。“保守氨基酸替换”为将氨基酸残基用具有相似侧链的氨基酸残基替换。具有相似侧链的氨基酸残基类已在本领域定义过。这些分类包括具有碱性侧链的氨基酸(例如，赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如，天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如，甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如，丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支侧链的氨基酸(例如，苏氨酸、缬氨酸、异亮氨酸)和具有芳香侧链的氨基酸(例如，酪氨酸、苯丙氨酸、色氨酸、组氨酸)。因此，在SMP蛋白中预测的非必需氨基酸残基优选地用来自同一侧链类的另一氨基酸残基替换。在另一实施方案中，可如通过饱和诱变沿着全部或部分SMP编码序列随机导入突变，并对所得突变体筛选文中所述的SMP活性，以鉴定保留SMP活性的突变体。对任一集合A的序列诱变后，可重组表达编码蛋白，并用例如文中所述的试验(参见实施例部分的实施例8)确定所述蛋白质的活性。

B.重组表达载体和宿主细胞

本发明的另一方面涉及包含编码SMP蛋白(或其部分)的核酸或包含基因组合且其中至少一种基因编码SMP蛋白的载体，优选表达载体。如此处所用，术语“载体”是指这样的核酸分子，其可转运与其连接的另一核酸。一种类型的载体为“质粒”，是指其中可连接额外DNA片段的环状双链DNA环。另一种类型的载体为病毒载体，其中可将额外DNA片段连接入病毒基因组。某些载体可在它们所导入的宿主细胞中自主复制(例如，具有细菌复制起点的细菌载体和游离型哺乳动物载体)。其它载体(例如，非游离型哺乳动物载体)在导入宿主细胞后整合入宿主细胞的基因组，并因此随着宿主基因组而复制。而且，某些载体可指导与它们操作性连接的基因的表达。此类载体此处称为“表达载体”。一般，用于重组DNA技术中的表达载体通常为质粒形式。在本说明书中，“质粒”和“载体”可互换使用，因为质粒是最经常使用的载体形式。但是，本发明旨在包括其它形式的表达载体，如病毒载体(例如，复制缺陷型反转录病毒、腺病毒和腺相关病毒)，它们起到类似的作用。

本发明的重组表达载体以适于在宿主细胞中表达核酸的形式包含本发明的核酸，这就是说重组表达载体包括一个或多个调节序列，其选择是基于用作表达的宿主细胞进行的，它们可操作地与待表达的核酸序列连接。在重组表达载体中，术语“功能性地连接”是指目的核苷酸序列与调节序列以允许该核苷酸序列表达(例如，在体外转录/翻译系统中，或当载体导入宿主细胞时在宿主细胞中表达)的方式连接。术语“调节序列”是指包括启动子、增强子和其它表达调控元件(例如，多聚腺苷酸化信号)。此类调节序列如在Goeddel；基因表达技术：酶学方法(GeneExpressionTechnology：Methods in Enzymology)185，Academic Press，SanDiego，CA(1990)中描述。调节序列包括在许多类型宿主细胞中控制核苷酸序列组成型表达的调节序列和仅在某些宿主细胞中控制核苷酸序列直接表达的调节序列。本领域普通技术人员了解到表达载体的设计可取决于如对欲转化宿主细胞的选择、所需的蛋白质表达程度等因素。本发明的表达载体可导入宿主细胞，以产生由此处所述核酸所编码的蛋白质或肽，包括融合蛋白或融合肽(例如，SMP蛋白、SMP蛋白的突变形式、融合蛋白等)。

本发明的重组表达载体可设计用于在原核或真核细胞中表达SMP蛋白。例如，SMP基因可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞(使用杆状病毒表达载体)、酵母细胞和其它真菌细胞(参见Romanos，M.A.等人(1992)“在酵母中外源基因的表达：综述”(“Foreign gene Expression inyeast：a review”)，酵母(Yeast)8：423-488；J.W.Bennet & L.L.Lasure编辑的更多的用于真菌的基因操作(More Gene Manipulations in Fungi)，Academic Press：San Diego一书中的van den Hondel，C.A.M.J.J.等人(1991)“丝状真菌中的异源基因表达”(“Heterologous gene Expression infilamentous fungi”)，396-428页；以及Peberdy，J.F.等人编辑的真菌的应用分子遗传学(Applied Molecular Genetics of Fungi)，CambridgeUniversity Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(Gene transfersystems and vector development for filamentous fungi)，1-28页)、藻类和单细胞植物细胞(参见Schmidt，R.和Willmitzer，L.(1988)，根瘤农杆菌介导的在鼠耳芥叶和子叶外植体中的高效转化，植物细胞报道(Plant CellRep.)：583-586)、或哺乳动物细胞中表达。合适的宿主细胞在Goeddel，基因表达技术：酶学方法(Gene Expression Technology：Methods inEnzymology)185，Academic Press，San Diego，CA(1990)中进一步进行了讨论。另外，可体外转录并翻译重组表达载体，如使用T7启动子调节序列和T7聚合酶。

在原核生物中蛋白质的表达主要用含有组成型或诱导型启动子的载体进行，控制融合或非融合蛋白的表达。融合载体将一些氨基酸添加至其中编码的蛋白质上，通常添加至重组蛋白的氨基末端。此类融合载体一般具有三个任务：1)用于增强重组蛋白的表达；2)用于增加重组蛋白的溶解性；和3)在亲和层析中作为配体而利于重组蛋白的纯化。通常，于融合部分和重组蛋白的连接处导入蛋白酶剪切位点到融合表达载体中，这使得在该融合蛋白纯化后，可将重组蛋白从融合单元分离。这些酶和它们的对应识别序列包括Xa因子、凝血酶和肠激酶。

通常的融合表达载体包括pGEX(Pharmacia Biotech Inc；Smith，D.B.和Johnson，K.S.(1988)基因(Gene)67：31-40)、pMAL(New EnglandBiolabs，Beverly，MA)和pRIT5(Pharmacia，Piscataway，NJ)，它们分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白和蛋白A结合至重组靶蛋白。在一个实施方案中，将SMP蛋白的编码序列克隆入pGEX表达载体，以产生编码融合蛋白的载体，其从N-末端至C-末端包含GST-凝血酶切割位点-X蛋白。该融合蛋白使用谷胱甘肽-琼脂糖树脂通过亲和层析纯化。通过将该融合蛋白用凝血酶切割可得到不与GST融合的重组SMP蛋白。

合适的诱导型非融合大肠杆菌表达载体的实例包括pTrc(Amann等人(1988)基因(Gene)69：301-315)和pET 11d(Studier等人，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)60-89)。pTrc载体中目标基因的表达依赖于宿主RNA聚合酶从杂合的trp-lac融合启动子的转录。pET 11d载体中目标基因的表达依赖于共表达的病毒RNA聚合酶(T7 gn1)介导的从T7-gn10-lac融合启动子的转录。该病毒聚合酶由宿主菌株BL21(DE3)或HMS174(DE3)中居住的、具有位于lacUV 5启动子转录调控下的T7 gn1基因的λ前噬菌体提供。

最大化重组蛋白表达的一个策略是将该蛋白质在具有对所述重组蛋白进行蛋白酶切割的能力缺损的宿主细菌中表达(Gottesman，S.，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)119-128)。另一策略是改变欲插入表达载体的核酸的核酸序列，以使用于每一氨基酸的单个密码子为选择用于表达的细菌如谷氨酸棒状杆菌所优选使用的密码子(Wada等人(1992)核酸研究(Nucleic Acids Res.)20：2111-2118)。本发明核酸序列的此改变可应用标准的DNA合成技术实施。

在另一实施方案中，SMP蛋白表达载体为酵母表达载体。用于在酿酒酵母中表达的载体包括pYepSec1(Baldari，等人(1987)Embo J.6：229-234)，pMFa(Kurjan和Herskowitz，(1982)细胞(Cell)30：933-943)，pJRY88(Schultz等人(1987)基因(Gene)54：113-123)，和pYES2(InvitrogenCorporation，San Diego，CA)。适用于其它真菌如丝状真菌的载体和用于构建载体的方法包括在：J.F.Peberdy等人编辑的真菌的应用分子遗传学(Applied Molecular Genetics of Fungi)，Cambridge University Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(Gene transfer systems and vectordevelopment for filamentous fungi)，1-28页中详述的那些。

另外，本发明的SMP蛋白可使用杆状病毒表达载体在昆虫细胞中表达。可用于在培养的昆虫细胞(例如Sf 9细胞)中表达蛋白质的杆状病毒载体包括pAc系列(Smith等人(1983)Mol.Cell Biol.3：2156-2165)和pVL系列(Lucklow和Summers(1989)病毒学(Virology)170：31-39)。

在另一实施方案中，本发明的SMP蛋白可在单细胞植物细胞(如藻类)或在来自高等植物(如种子植物，诸如作物)的植物细胞中表达。植物表达载体的实例包括在：Becker，D.，Kemper，E.，Schell，J.和Masterson，R.(1992)“具有靠近左边界的选择性标记的新植物双元载体(“New plantbinary vectors with selectable markers located proximal to the leftborder”)，植物分子生物学(Plant Mol.Biol.)20：1195-1197；和Bevan，M.W.(1984)“用于植物转化的双元农杆菌载体”(“Binary Agrobacteriumvectors for plant transformation”)，核酸研究(Nucl.Acid.Res.)12：8711-8721中详述的那些。

在另一实施方案中，本发明的核酸用哺乳动物表达载体在哺乳动物细胞中表达。哺乳动物表达载体的实例包括pCDM8(Seed，B.(1987)自然(Nature)329：840)和pMT2PC(Kaufman等人(1987)EMBO J.6：187-195)。当用于哺乳动物细胞时，表达载体的调控功能通常由病毒调控元件提供。例如，常用的启动子来自多瘤、腺病毒2、巨细胞病毒和猿猴病毒40。用于原核和真核细胞的其它合适表达系统参见Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：ALaboratory Manual)，第二版，Cold Spring Harbor Laboratory，ColdSpring Harbor Laboratory Press，Cold Spring Harbor，NY，1989中的第16和17章。

在另一实施方案中，重组哺乳动物表达载体可优选地在特定细胞类型中指导核酸的表达(例如，使用组织-特异性调控元件来表达核酸)。组织-特异性调控元件为本领域公知。合适的组织-特异性启动子的非限制性实例包括白蛋白启动子(肝-特异性；Pinkert等人(1987)基因进展(Genes Dev.)1：268-277)、淋巴-特异性启动子(Calame and Eaton(1988)Adv.Immunol.43：235-275)，特别是T细胞受体启动子(Winoto和Baltimore(1989)EMBOJ.8：729-733)和免疫球蛋白启动子(Banerji等人(1983)细胞(Cell)33：729-740；Queen和Baltimore(1983)细胞(Cell)33：741-748)、神经元-特异性启动子(例如，神经丝启动子；Byrne和Ruddle(1989)PNAS86：5473-5477)、胰-特异性启动子(Edlund等人(1985)科学(Science)230：912-916)、以及乳腺-特异性启动子(例如，奶乳清启动子；美国专利号4,873,316和欧洲申请公开号264,166)。也同样包括调节发育的启动子，如鼠hox启动子(Kessel和Gruss(1990)科学(Science)249：374-379)和甲胎蛋白启动子(Campes和Tilghman(1989)基因进展(Genes Dev.)3：537-546)。

本发明还提供了含本发明的DNA分子的重组表达载体，其中该DNA分子以反义方向克隆在表达载体中。也就是，DNA分子功能性地连接到调控序列上，该连接方式使得可以表达产生与SMP mRNA反义的RNA分子(通过转录该DNA分子)。可以对功能性地连接反义方向克隆的核酸的调控序列进行选择以控制反义RNA分子在多种细胞类型中持续表达，例如，可选择病毒启动子和/或增强子或调控序列以控制反义RNA实现组成型组织特异性或细胞类型特异性表达。反义表达载体的形式可为重组质粒、噬菌粒或减毒病毒，其中在高效调控区域的控制下产生反义核酸，其活性由载体所导入细胞的类型决定。反义基因的基因表达调控的讨论见Weintraub，H.等人，反义RNA作为遗传分析的分子工具，Reviews-Trendsin Genetics，卷1(1)1986。

本发明的另一方面涉及已导入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在此处可互换使用。当然，该术语不仅指特定的受试细胞，也指该细胞的后代或潜在后代。由于突变或环境因素在传代过程中可发生某些改变，这样，后代实际上可能并非与亲代细胞相同，但其仍包括在此处所用术语的范围之内。

宿主细胞可为任意的原核或真核细胞。例如，SMP蛋白可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞、酵母或哺乳动物细胞(如中国仓鼠卵巢(CHO)细胞或COS细胞)中表达。其他合适的宿主细胞为技术人员公知。与谷氨酸棒状杆菌有关的、可常规用作本发明核酸和蛋白质分子的宿主细胞的微生物如表3所示。

载体DNA可通过常规转化或转染技术导入原核或真核细胞中。如此处所用，术语“转化”和“转染”意指本领域内公知的各种将外源核酸(例如DNA)导入宿主细胞的技术，包括磷酸钙或氯化钙共沉淀、DEAE-葡聚糖-介导的转染、脂转染、电穿孔。转化或转染宿主细胞的合适方法可在Sambrook等人(分子克隆：实验室手册(Molecular Cloning：A LaboratoryManual).第二版，Cold Spring Harbor Laboratory，Cold Spring HarborLaboratory Press，Cold Spring Harbor，NY，1989)和其它的实验室手册中找到。

对于哺乳动物细胞的稳定转染，已知根据所用的表达载体和所用的转染技术，仅一小部分的细胞可将外源DNA整合到基因组中。一般将编码选择标记的基因(如抗生素抗性基因)与目的基因一同导入宿主细胞以鉴定和筛选整合体。优选的选择标记包括那些可赋予药物抗性的基因，所述药物如G418、潮霉素和氨甲蝶呤。编码选择标记的核酸可与编码SMP蛋白质的基因位于同一载体上被导入宿主细胞或可以位于不同的载体上导入。经导入核酸稳定转染的细胞可通过药物筛选进行鉴定(例如，掺入了选择标志基因的细胞将存活，而其他细胞死亡)。

通过制备出含至少部分SMP基因的载体，所述部分SMP基因中已经导入缺失、添加或替代因而可以改变SMP基因(如功能性破坏)来生成同源重组微生物。优选地该SMP基因为谷氨酸棒状杆菌SMP基因，但也可为来自相关细菌或甚至来自哺乳动物、酵母或昆虫的同系物。在一个优选的实施方案中，载体设计为在同源重组后可功能性破坏内源SMP基因(即，不再编码功能蛋白质；也称为“敲除”载体)。另外，载体可设计为在同源重组后内源SMP基因发生突变或改变但仍编码功能蛋白质(例如，可改变上游调控区域由此改变内源SMP蛋白的表达)。在此同源重组载体中，改变的SMP基因部分在其5′和3′端侧翼为SMP基因的其它核酸，从而允许在载体携带的外源SMP基因和微生物中的内源SMP基因之间发生同源重组。此其它的两翼SMP核酸的长度应足以使与内源基因的重组成功进行。通常，载体内包括几千碱基的侧翼DNA(5′及3′端)(参见例如，Thomas，K.R.，和Capecchi，M.R.(1987)细胞(Cell)51：503中对同源重组载体的描述)。使用本领域公知的方法，将该载体导入微生物(例如，通过电穿孔)和细胞，筛选其中导入的SMP基因已与内源SMP基因同源重组的微生物和细胞。

在另一实施方案中，产生的重组微生物可含有可调控导入基因表达的所选系统。例如，将在载体中包含的SMP基因插入乳糖操纵子的控制下，使得SMP基因仅在存在IPTG时表达。此类调控系统为本领域熟知。

本发明的宿主细胞如培养的原核或真核宿主细胞可用于产生(即表达)SMP蛋白。因此，本发明还提供了使用本发明的宿主细胞来产生SMP蛋白的方法。在一个实施方案中，该方法包括在合适的培养基中培养本发明的宿主细胞(其中已导入了编码SMP蛋白的重组表达载体，或其中基因组已导入了编码野生型或改变的SMP蛋白的基因)，直至产生SMP蛋白。在另一实施方案中，该方法还包括从培养基或宿主细胞分离SMP蛋白。

C.本发明的用途和方法

文中所述的核酸分子、蛋白质、蛋白质同系物、融合蛋白、引物、载体和宿主细胞可用于一种或多种下述方法中：鉴定谷氨酸棒状杆菌及相关生物体；绘制与谷氨酸棒状杆菌相关的生物体的基因组图谱；鉴定并定位目标谷氨酸棒状杆菌序列；进化研究；确定为SMP蛋白所需的功能区域；调节SMP蛋白活性；调节一种或多种糖的代谢；调节细胞中高能分子的产生(即，调节ATP、NADPH的产生)；以及调节目标化合物如精细化学品的细胞生产率。本发明的SMP核酸分子具有多种用途。首先，它们可用于鉴定生物体为谷氨酸棒状杆菌或其近亲。它们也可用于在混合的微生物群体中鉴定谷氨酸棒状杆菌或其相关菌的存在。本发明提供了许多谷氨酸棒状杆菌基因的核酸序列。通过将微生物单一群体或混合群体的培养物提取的基因组DNA在严格条件下与包含对谷氨酸棒状杆菌独特的谷氨酸棒状杆菌基因区域的探针杂交，可确定该生物体是否存在。虽然谷氨酸棒状杆菌自身为非致病的，但它与致病种如白喉棒状杆菌有亲缘关系。这种生物体的检测具有显著的临床重要性。

本发明的核酸和蛋白质分子也可用作基因组特异性区域的标记。它不仅可用于基因组图谱，也可用于谷氨酸棒状杆菌蛋白质的功能研究。例如，可切割谷氨酸棒状杆菌基因组，并将片段与DNA-结合蛋白孵育来确定与特定的谷氨酸棒状杆菌DNA-结合蛋白结合的基因组区域。另外，那些结合蛋白的片段可用本发明的核酸分子探测，优选地用具有易于检测的标记物的核酸分子探测；此核酸分子与基因组片段的结合可将片段定位于谷氨酸棒状杆菌的基因组图谱中，并且当用不同的酶进行多次时，利于快速确定蛋白质结合的核酸序列。而且，本发明的核酸分子可充分同源于亲缘种的序列，这样这些核酸分子可用作在亲缘菌如乳糖发酵短杆菌中构建基因组图谱的标记。

本发明的SMP核酸分子也用于进化研究和蛋白质结构研究。大量的原核和真核细胞应用本发明分子参与的代谢过程；通过将本发明核酸分子的序列与那些来自其它生物体的编码类似酶的序列比较，可评估所述生物体的进化关系。所以，此比较使得可评估哪些区域保守和哪些区域不保守，这有利于确定那些对酶功能所必需的蛋白质区域。这种确定对蛋白质工程研究有价值，并可指出该蛋白质可耐受何种诱变且不丢失其功能。

本发明SMP核酸分子的操作可导致产生与野生型SMP蛋白功能不同的SMP蛋白。这些蛋白质可在功效或活性上提高，在细胞内可以较通常多的量存在，或可在功效或活性上降低。

通过许多机制修饰本发明的SMP蛋白可直接影响包含该经修饰蛋白的谷氨酸棒状杆菌中精细化学品的产量、生产率和/或生产效率。高能碳分子如糖的降解以及像NADH和FADH₂这样的化合物通过氧化磷酸化转化为更有用的化合物导致许多化合物如丙酮酸、ATP、NADH和许多糖中间体自身即为所希望的精细化学品。而且，这些代谢途径所产生的能量分子(如ATP)以及还原型等同物(如NADH或NADPH)在细胞中用于驱动细胞中能量逆差的反应。此类能量逆差的反应包括精细化学品的许多生物合成途径。通过提高细胞利用特定糖的能力(例如，通过操纵细胞中参与所述糖降解并转化为能量的基因)，可增加用于进行能量逆差的、但为所需要的代谢反应(如目标精细化学品的生物合成)的能量。

而且，对参与糖利用的一个或多个途径的调节可优化包含在糖分子中的能量转化，用于产生一种或多种精细化学品。例如，对参与如葡糖异生的酶活性降低导致细胞可获得更多的ATP用于驱动目标生物化学反应(如精细化学品的生物合成)。也可调节来自糖的能量分子的总生产率，以确保细胞对来自每一糖分子的能量产生最大化。对糖的非有效利用可导致过多的CO₂和过多的能量产生，从而引起无价值的代谢循环。改善糖分子的代谢应使得细胞能更有效地发挥作用并使用较少的碳分子。这导致精细化学品∶糖分子的比率提高(提高的碳产率)，并使得在大规模发酵培养经这种方式修饰的谷氨酸棒状杆菌时可减少必须加入培养基中的糖量。

诱变本发明的一个或多个SMP蛋白也可产生活性改变的SMP蛋白，其中所述活性改变的SMP蛋白间接影响谷氨酸棒状杆菌中一种或多种目标精细化学品的生产率。例如，可通过提高对一种或多种糖的利用效率(以便增加将所述糖转化为可利用的能量分子)或通过增加将还原型等同物转化为可利用能量分子的效率(例如，通过提高氧化磷酸化效率或ATP合酶的活性)，从而增加这些高能化合物的量，其中所述高能化合物可用于细胞驱动通常为能量逆差的代谢过程。这些过程包括细胞壁的构建、转录、翻译以及细胞生长和细胞分裂所需的化合物(如核苷酸、氨基酸、维生素、脂类等)的生物合成(Lengeler等(1999)，原核生物学(Biology ofProkaryotes)，Thieme Verlag：Stuttgart，88-109页；913-918页；875-899页)。这些经修饰细胞生长和增殖的改善使得可在大规模培养物中增加所述细胞的成活力并且可提高它们的分裂速率，从而相对大量的细胞可在发酵培养物中存活。产量、生产率或生产效率可得以提高，这至少是由于存在大量在所有情况下均产生目标精细化学品的活细胞。

而且，在糖代谢中产生的许多降解产物本身也被细胞用作产生许多其它有用化合物的前体或中间体，其中一部分本身为精细化学品。例如，丙酮酸转化为丙氨酸，5-磷酸核糖为构成例如核苷酸分子所需的成分。因此糖代谢的程度和效率对细胞中这些降解产物的可用性有深刻的影响。通过提高现存途径的效率(例如通过修饰参与这些途径中的酶而优化酶活性)或通过提高参与所述途径的酶的可用性(例如通过增加细胞中存在的酶的数量)来提高细胞加工糖的能力，从而使得可增加所述降解产物在细胞中的可用性，接下来增加了细胞中多种其它所希望化合物(例如精细化学品)的生产率。

上述用于增加谷氨酸棒状杆菌目标化合物产量的SMP蛋白的诱变策略不用于限制；对这些诱变策略的变化对技术人员是显而易见的。本文公开的这些策略和机制使得可以使用本发明的核酸和蛋白质分子以产生表达突变的SMP核酸和蛋白质分子的谷氨酸棒状杆菌或相关的细菌菌株，从而提高目标化合物的产量、生产率和/或产生效率。该目标化合物可为谷氨酸棒状杆菌的任一天然产物，其包括生物合成途径的终产物和天然代谢途径的中间体，该目标化合物也可为在谷氨酸棒状杆菌代谢中非天然存在的分子，但该分子为本发明的谷氨酸棒状杆菌菌株产生。

不应将下面的实施例理解为对限制对本发明的进一步阐述。在该申请中引用的所有参考文献、专利申请、专利和公开的专利申请的全部内容此处引用作为参考。

实施例

实施例1：谷氨酸棒状杆菌ATCC 13032的总基因组DNA的制备

谷氨酸棒状杆菌(ATCC 13032)的培养物在30℃于BHI培养基(Difco)中剧烈振摇过夜。离心收获细胞，弃上清并将细胞重悬于5ml缓冲液-I(培养物原初体积的5％—所有陈述的体积按100ml培养物体积计算)。缓冲液-I的组成：140.34g/l蔗糖，2.46g/l MgSO₄·7H₂O，10ml/l KH₂PO₄溶液(100g/l，用KOH调节至pH 6.7)，50ml/l M12浓缩物(10g/l(NH₄)₂SO₄，1g/lNaCl，2g/l MgSO₄·7H₂O，0.2g/l CaCl₂，0.5g/l酵母提取物(Difco)，10ml/l痕量元素混合物(200mg/l FeSO₄·H₂O，10mg/l ZnSO₄·7 H₂O，3mg/lMnCl₂·4 H₂O，30mg/l H₃BO₃，20mg/l CoCl₂·6H₂O，1mg/l NiCl₂·6 H₂O，3mg/l Na₂MoO₄·2 H₂O，500mg/l络合剂(EDTA或柠檬酸)，100ml/l维生素-混合物(0.2mg/l生物素，0.2mg/l叶酸，20mg/l对氨基苯甲酸，20mg/l核黄素，40mg/l Ca-泛酸盐，140mg/l烟酸，40mg/l维生素B6盐酸盐，200mg/l肌醇)。向悬液中加入溶菌酶至终浓度2.5mg/ml。在37℃孵育约4小时后，细胞壁降解，离心收获所得的原生质体。沉淀用5ml缓冲液-I洗一次并用5ml TE-缓冲液(10mM Tris-HCl，1mM EDTA，pH 8)洗一次。将沉淀重悬于4ml TE-缓冲液，并加入0.5ml SDS溶液(10％)和0.5ml NaCl溶液(5M)。加入蛋白酶K至终浓度200μg/ml后，将悬液在37℃孵育约18小时。使用标准方法用酚、酚-氯仿-异戊醇和氯仿-异戊醇提取而纯化DNA。然后，加入1/50体积的3M醋酸钠和2体积的乙醇，于-20℃孵育30分钟，并在高速离心机上使用SS34转子(Sorvall)于12,000转/分离心30分钟，沉淀DNA。将该DNA溶于含20μg/ml RNaseA的1ml TE-缓冲液中，并用1000ml TE-缓冲液于4℃透析至少3小时。在此期间，更换缓冲液3次。向分装的0.4ml透析过的DNA溶液中加入0.4ml 2M LiCl和0.8ml乙醇。于-20℃孵育30分钟后，离心(13,000转/分，Biofuge Fresco，Heraeus，Hanau，德国)收集DNA。将该DNA沉淀溶于TE-缓冲液。用此方法制备的DNA可用于所有目的，包括Southern印迹或构建基因组文库。

实施例2：在大肠杆菌中构建谷氨酸棒状杆菌(ATCC13032)的基因组文库

从如实施例1所述制备的DNA开始，按照公知的且已建立的方法(参见例如，Sambrook，J.等人(1989)“分子克隆：实验室手册”(”MolecularCloning：A Laboratory Manual”)，Cold Spring Harbor Laboratory Press，或Ausubel，F.M.等人(1994)“分子生物学最新方法”(”Current Protocolsin Molecular Biology”)，John Wiley & Sons.)构建粘粒和质粒文库。

可使用任一质粒或粘粒。特别使用的是质粒pBR322(Sutcliffe，J.G.(1979)美国国家科学院院报(Proc.Natl.Acad.Sci.USA)，75：3737-3741)；pACYC177(Change & Cohen(1978)细菌学杂志(J.Bacteriol)134：1141-1156)、pBS系列的质粒(pBSSK+，pBSSK-及其它；Stratagene，LaJolla，USA)、或粘粒如SuperCos1(Stratagene，LaJolla，USA)或Lorist6(Gibson，T.J.，Rosenthal A.和Waterson，R.H.(1987)基因(Gene)53：283-286)。

实施例3：DNA序列测定和功能的计算分析

如实施例2所述的基因组文库按照标准方法用于DNA序列测定，尤其是使用ABI377序列测定仪通过链终止法测定(参见例如，Fleischman，R.D.等人(1995)“流感嗜血菌(Haemophilus Influenzae)Rd的全基因组随机序列测定和组装”，科学(Science)，269：496-512)。使用具有以下核苷酸序列的测序引物：5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。

实施例4：体内诱变

谷氨酸棒状杆菌的体内诱变可通过将质粒(或其它载体)DNA在不能维持其遗传信息完整性的大肠杆菌或其它微生物(例如Bacillus spp.或酵母如酿酒酵母)中传代而实施。常见的突变基因菌株在用于DNA修复系统的基因中有突变(例如mutHLS，mutD，mutT等；参考文献见大肠杆菌和沙门菌(Escherichia coli and Salmonella)，ASM：Washington一书中的Rupp，W.D.(1996)DNA修复机制，2277-2294页)。此类菌株为技术人员熟知。此类菌株的用途在例如Greener，A.和Callahan，M.(1994)Strategies 7：32-34中进行了阐述。

实施例5：在大肠杆菌和谷氨酸棒状杆菌之间的DNA转移

几种棒状杆菌和短杆菌种类含有自主复制的内源质粒(例如pHM1519或pBL1)(综述参见例如，Martin，J.F.等人(1987)生物技术(Biotechnology)，5：137-146)。使用标准的大肠杆菌载体易于构建大肠杆菌和谷氨酸棒状杆菌的穿梭载体(Sambrook，J.等人(1989)，“分子克隆：实验室手册”(“Molecular Cloning：A Laboratory Manual”)，Cold Spring HarborLaboratory Press，或Ausubel，F.M.等人(1994)“最新分子生物学方法”(“Current Protocols in Molecular Biology”)，John Wiley & Sons)，其中加入复制原点和来自谷氨酸棒状杆菌的适当标记。此类复制原点优选地取自从棒状杆菌和短杆菌分离的内源质粒。这些种类中作为转化标记尤其有用的是卡那霉素抗性基因(如衍生自Tn5或Tn903转座子的基因)或氯霉素抗性基因(Winnacker，E.L.(1987)”From Genes to Clones-Introductionto Gene Technology，VCH，Weinheim)。在文献中有许多在大肠杆菌和谷氨酸棒状杆菌中均复制的多种穿梭载体的例子，且其可用于多个目的，包括基因过表达(参考文献参见例如，Yoshihama，M.等人(1985)细菌学杂志(J.Bacteriol.)162：591-597，Martin J.F.等人(1987)生物技术(Biotechnology)，5：137-146和Eikmanns，B.J.等人(1992)基因(Gene)，102：93-98)。

使用标准技术，可将目的基因克隆入上述的穿梭载体之一并可将此杂合载体导入谷氨酸棒状杆菌菌株。谷氨酸棒状杆菌的转化可通过原生质体转化(Kastsumata，R.等人(1984)细菌学杂志(J.Bacteriol.)159，306-311)、电穿孔(Liebl，E.等人(1989)FEMS Microbiol.Letters，53：399-303)以及当使用特别的载体时，也通过接合(如在Sch_fer，A等人(1990)细菌学杂志(J.Bacteriol.)172：1663-1666中所述)来完成。也可转移谷氨酸棒状杆菌的穿梭载体至大肠杆菌，其为通过制备来自谷氨酸棒状杆菌的质粒DNA(使用本领域公知的标准方法)并将其转化至大肠杆菌。该转化步骤可使用标准方法进行，但有利的是使用Mcr-缺陷的大肠杆菌菌株，如NM522(Gough & Murray(1983)分子生物学杂志(J.Mol.Biol.)166：1-19)。

实施例6：突变蛋白质的表达的确定

在转化的宿主细胞中对突变蛋白质活性的观察依赖于突变蛋白质以与野生型蛋白质相似的方式和相似的量表达的事实。确定突变基因转录水平(指示可用于翻译为基因产物的mRNA量)的有用方法是进行Northern印迹(参考文献见例如，Ausubel等人(1988)分子生物学最新方法(CurrentProtocols in Molecular Biology)，Wiley：New York)，其中设计为与目的基因结合的引物用可检测标志(通常为放射性的或化学发光的)标记，这样当提取生物体培养物的总RNA、在凝胶上运行、转移至稳定的基质并与该探针孵育时，探针的结合和结合量指示了该基因的存在和其mRNA量。该信息是突变基因转录程度的证据。通过几种方法可从谷氨酸棒状杆菌中分离总细胞RNA，这些方法均为本领域熟知，如在Bormann，E.R.等人(1992)分子微生物学(Mol.Microbiol.)6：317-326中所描述。

为了确定从该mRNA翻译的蛋白质的存在或相对量，可使用标准技术如Western印迹(参见例如，Ausubel等人(1988)分子生物学最新方法(Current Protocols in Molecular Biology)，Wiley：New York)。在该方法中，提取细胞总蛋白质、通过凝胶电泳分离、转移至基质如硝化纤维并与探针孵育，其中所述探针如与目标蛋白质特异性结合的抗体。该探针通常用易于检测到的化学发光或显色标记物标记。所观察到的标记物的存在和量指示了在细胞中目标突变蛋白质的存在和量。

实施例7：遗传修饰的谷氨酸棒状杆菌的生长—培养基和培养条件

遗传修饰的棒状杆菌培养在合成或天然的生长培养基中。用于棒状杆菌的许多种不同生长培养基为人们熟知并易于获得(Lieb等人(1989)Appl.Microbiol.Biotechnol.，32：205-210；von der Osten等人(1998)Biotechnology Letters，11：11-16；Patent DE 4,120,867；在原核生物(TheProcaryotes)，Volume II，Balows，A.等人编辑.Springer-Verlag一书中的Liebl(1992)“棒状杆菌属”(“The Genus Corynebacteria”))。这些培养基由一种或多种碳源、氮源、无机盐、维生素和痕量元素组成。优选的碳源是糖类，如单糖、二糖或或多糖。优良碳源的实例是葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉和纤维素。也可通过复合化合物如糖蜜或其它来自糖加工的副产品提供糖至培养基。提供不同碳源的混合物也可是有利的。其它可能的碳源为醇类和有机酸，如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物，或为包含这些化合物的物质。示例性氮源包括氨气或胺盐如NH₄Cl或(NH₄)₂SO₄、NH₄OH、硝酸盐、尿素、氨基酸和复合氮源如玉米浆、大豆粉、大豆蛋白质、酵母提取物、肉羹等。

可包括在培养基中的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的氯化物、亚磷酸盐或硫酸盐。可向培养基中加入螯合物以维持溶液中的金属离子。尤其有用的螯合物包括二羟基酚，如儿茶酚或原儿茶酚盐，和有机酸如柠檬酸。培养基一般也包括其它生长因子如维生素或生长促进剂，其实例包括生物素、核黄素、硫胺素、叶酸、烟酸、泛酸盐和维生素B₆。生长因子和盐通常来自复合的培养基组成，如酵母提取物、糖蜜、玉米浆等。培养基化合物的精确组成强烈取决于直接实验并在每一具体情况下单独确定。有关培养基优化的信息在课本“Applied Microbiol.Physiology，A Practical Approach”(P.M.Rhodes，P.F.Stanbury编辑，IRL Press(1997)53-73页，ISBN 0 19 963577 3)中可找到。也可从供应商处得到生长培养基，如Standard 1(Merck)或BHI(brain heart infusion，DIFCO)和其它。

通过加热(1.5巴，121℃加热20分钟)或过滤除菌对所有的培养基组成成份进行灭菌。组成成份可一起灭菌，或需要的话分开灭菌。所有的培养基组成成份可在培养开始时存在，或它们可任选地连续加入或分批加入。

对每一实验分别定义培养条件。温度应在15℃和45℃的范围之间。在实验中温度可保持恒定或可改变。培养基的pH应在5至8.5的范围之间，优选地在7.0附近，可通过向培养基中添加缓冲液而维持。用于此目的的示例性缓冲液为磷酸钾缓冲液。可备选地或同时使用合成缓冲液如MOPS、HEPES、ACES和其它。在生长过程中通过加入NaOH或NH₄OH也可维持恒定的培养pH。如果使用复合的培养基组成如酵母提取物，可减轻对额外的缓冲液的需要，因为许多复合化合物具有高缓冲能力。如果使用发酵罐培养微生物，用气态氨也可控制pH。

培养时间通常在几小时到几天之间。选择培养时间以允许肉汤中累积最大量的产物。可在多种容器如不同规格的微滴定板、玻璃管、玻璃烧瓶或玻璃或金属发酵罐中实施公开的生长实验。对于筛选大量的克隆，应在微滴定板、玻璃管或在有或没有挡板的摇瓶中培养微生物。优选地使用100ml摇瓶，其中装有10％(体积)的所需生长培养基。该烧瓶应在旋转振荡器(振幅25mm)上以100-300转/分摇动。可通过维持潮湿环境减少蒸发损失；备选地，对蒸发损失应进行数学上的校正。

如果试验遗传修饰的克隆，也应试验未修饰的对照克隆或含基本质粒的无任何插入的对照克。使用已于30℃孵育的在琼脂板上生长的细胞接种培养基至OD₆₀₀ 0.5-1.5，其中所述琼脂板如CM板(10g/l葡萄糖，2.5g/lNaCl，2g/l尿素，10g/l聚胨，5g/l酵母抽取物，5g/l肉羹，22g/l琼脂，用2M NaOH调节至pH 6.8)。培养基的接种通过导入来自CM板的谷氨酸棒状杆菌细胞的盐水悬液或添加该细菌的原液体培养物而完成。

实施例8：突变蛋白质功能的体外分析

本领域已建立了对酶活性和动力学的确定。确定特定的改变酶的活性的实验应加以修改，使适应野生型酶特异活性的确定，这在本领域普通技术人员的能力范围内。可在下列参考文献中找到对酶从总体上的概观，以及有关结构、动力学、原理、方法、应用和对许多酶活性进行确定的例子的具体说明，例如Dixon，M.和Webb，E.C.，(1979)酶(Enzymes).Longmans：伦敦；Fersht，(1985)酶结构和机制(Enzyme Structure andMechanism).Freeman：纽约；Walsh，(1979)酶促反应机制(EnzymaticReaction Mechanisms).Freeman：San Francisco；Price，N.C.，Stevens，L.(1982)酶学基础(Fundamentals of Enzymology).Oxford Univ.Press：Oxford；Boyer，P.D.编辑(1983)酶(The Enzymes)，第三版，Academic Press：纽约；Bisswanger，H.，(1994)酶动力学(Enzymkinetik)，第二版.VCH：Weinheim(ISBN 3527300325)；Bergmeyer，H.U.，Bergmeyer，J.，Graβl，M.编辑(1983-1986)酶促分析方法(Methods of Enzymatic Analysis)，第三版，I-XII卷，Verlag Chemie：Weinheim；以及Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)(1987)A9卷，“酶”.VCH：Weinheim，352-363页。

可通过几种已建立的方法测定结合DNA的蛋白质的活性，如DNA带移动试验(也称为凝胶阻滞试验)。此类蛋白质对其它分子表达的影响可使用报告基因试验测定(如在Kolmar，H.等人(1995)EMBO J.14：3895-3904及其中所引用的参考文献中所述)。报告基因测试系统为人们所熟知，并被建立以应用于原核和真核细胞，使用的酶如β-半乳糖苷酶、绿色荧光蛋白和几种其它的酶。

可根据如在生物膜，分子结构和功能(Biomembranes，MolecularStructure and Function)，Springer：Heidelberg一书中的Gennis，R.B.(1989)”Pores，Channels and Transporters”，85-137；199-234和270-322页中所述的技术进行膜转运蛋白活性的测定。

实施例9：分析突变蛋白质对目标产物产生的影响

谷氨酸棒状杆菌中的遗传修饰对目标化合物(如氨基酸)产生的影响可通过将经修饰的微生物在合适的条件(如上文中所描述的那些条件)下生长并分析用于增加目标产物(即氨基酸)产生的培养基和/或细胞组成来确定。此类分析技术为技术人员熟知，包括光谱学、薄层色谱法、多种染色法、酶促和微生物学方法、以及分析色谱法如高效液相色谱法(参见例如，Ullman工业化学百科全书(Ullman，Encyclopedia of IndustrialChemistry)，A2卷，89-90和443-613页，VCH：Weinheim(1985)；Fallon，A.等人(1987)生物化学和分子生物学实验室技术(Laboratory Techniques inBiochemistry and Molecular Biology)中的“HPLC在生物化学中的应用”(“Applications of HPLC in Biochemistry”)，17卷；Rehm等人(1993)生物技术(Biotechnology)，3卷，III章“产物回收和纯化”(”Productrecovery and purification”)，469-714页，VCH：Weinheim；Belter，P.A.等人(1988)生物分离：生物技术中的下游加工(Bioseparations：downstreamprocessing for biotechnology)，John Wiley and Sons；Kennedy，J.F.和Cabral，J.M.S.(1992)生物物质的回收方法(Recovery processes forbiological materials)John Wiley and Sons；在Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)一书中的Shaeiwitz，J.A.和Henry，J.D.(1988)生物化学分离(Biochemical separations)，B3卷，11章，1-27页，VCH：Weinheim；以及Dechow，F.J.(1989)生物技术中的分离和纯化技术(Separation and purification techniques inbiotechnology)，Noyes Publications.)。

除了测定发酵终产物外，还可分析用于产生目标化合物的代谢途径的其它组成如中间体和副产物来确定化合物的总体产生力、化合物的产率和/或生产效率。分析方法包括测定培养基中的营养素水平(例如，糖类、碳水化合物、氮源、磷酸盐和其它离子)、测定生物量组成和生长、分析生物合成途径中共同代谢物的产生及测定发酵过程中产生的气体。这些测定的标准方法在应用微生物生理学，实用方法(Applied Microbial Physiology，APractical Approach)，P.M.Rhodes和P.F.Stanbury编辑，IRL Press，103-129；131-163和165-192页(ISBN：0199635773)及其引用的参考文献中列出。

实施例10：从谷氨酸棒状杆菌培养物中纯化目标产物

可通过多种本领域熟知的方法实施从谷氨酸棒状杆菌细胞或上述培养物上清回收目标产物。如果目标产物不从细胞中分泌，可低速离心从培养物中收获细胞、用标准技术如机械力或超声裂解细胞。离心去除细胞碎片，保留含有可溶蛋白质的上清级分用于进一步纯化目标化合物。如果化合物从谷氨酸棒状杆菌细胞分泌，则低速离心从培养物中去除细胞，并保留上清级分用于进一步纯化。

将来自两种中任一纯化方法的上清级分用合适的树脂进行层析，其中目标分子保留在层析树脂上而样本中的许多杂质不保留在层析树脂上，或者杂质保留在层析树脂上而样本不保留。此层析步骤必需时可重复，其中使用相同或不同的层析树脂。技术人员在选择合适的层析树脂以及将它们最有效地用于特定的待纯化分子方面是很精通的。可通过过滤或超滤浓缩纯化产物，并贮存在使产物稳定性最高的温度下。

有大量的纯化方法为本领域公知，上述纯化方法不用于限制。此类纯化技术例如在Bailey，J.E.和Ollis，D.F.生物化学工程基础(BiochemicalEngineering Fundamentals)，McGraw-Hill：纽约(1986)中描述。

分离化合物的特性和纯度可通过本领域的标准技术确定。这些技术包括高效液相色谱(HPLC)、光谱法、染色法、薄层色谱法、NIRS、酶促试验或微生物学方法。此类分析方法在Patek等人(1994)Appl.Environ.Microbiol.60：133-140；Malakhova等人(1996)生物技术(Biotekhnologiya)11：27-32；和Schmidt等人(1998)生物加工工程(Bioprocess Engineer)19：67-70.Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)(1996)A27卷，VCH：Weinheim，89-90页，521-540页，540-547页，559-566页，575-581页和581-587页；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，John Wiley and Sons；生物化学和分子生物学中的实验室技术(Laboratory Techniques in Biochemistry and Molecular Biology)，17卷中的Fallon，A.等人(1987)HPLC在生物化学中的应用(Applications ofHPLC in Biochemistry)。

等同物

技术人员知道或通过简单的常规方法可确定许多于文中所描述本发明具体实施方案的等同物。此类等同物旨在包括在下面的专利权利要求书中。

表1中的信息理解如下：

在第1列中，“DNA ID”，每种情况下相关数字指所公开序列表的SEQ ID NO。所以，“DNA ID”列中的“5”指SEQ ID NO：5。

在第2列中，“AA ID”，每种情况下相关数字指所公开序列的SEQ IDNO。“AA ID”列中的“6”指SEQ ID NO：6。

在第3列中，列出了“标识”——每个序列的明确内部名称。

在第4列中，“AA pos”，每种情况下相关数字指相同行中多肽序列“AA ID”的氨基酸位置。所以，“AA pos”列中“26”是相应所示的多肽序列的氨基酸位置26。位置计算从N末端的+1开始。

在第5列中，“AA野生型”，每种情况下相关数字指相应野生型菌株在第4列中所示位置的氨基酸(用单字母代码)。

在第6列中，“AA突变株”，每种情况下相关数字指相应突变菌株在第4列中所示位置的氨基酸(用单字母代码)。

在第7列中，列出了“功能”——相应多肽序列的生理学功能。

蛋白原氨基酸的单字母代码：

A 丙氨酸

C 半胱氨酸

D 天冬氨酸

E 谷氨酸

F 苯丙氨酸

G 甘氨酸

H 组氨酸

I 异亮氨酸

K 赖氨酸

L 亮氨酸

M 甲硫氨酸

N 天冬酰胺

P 脯氨酸

Q 谷氨酰胺

R 精氨酸

S 丝氨酸

T 苏氨酸

V 缬氨酸

W 色氨酸

Y 酪氨酸

表1

编码碳代谢和能量产生的蛋白质的基因

DNA ID： AA ID：标识 AA位置： AA野生型 AA突变体功能：

1 2 RXA00149 75 P S 甲基丙二酸单酰CoA变位酶(EC 5.4.99.2)

413 G E 甲基丙二酸单酰CoA变位酶(EC 5.4.99.2)

417 A V 甲基丙二酸单酰CoA变位酶(EC 5.4.99.2)

3 4 RXA00196 83 G E 5-磷酸1-脱氧-D-木酮糖还原异构酶(EC 1.1.1.-)

5 6 RXA00224 54 G S 电子转移黄素蛋白α亚单位

7 8 RXA00235 223 E K 烯醇酶(EC 4.2.1.11)

9 10 RXA00296 918 C Y 厌氧性3-磷酸甘油脱氢酶亚单位C/甘醇酸酯氧化酶，铁硫亚单位

11 12 RXA00389 55 A T 氧代戊二酸半醛脱氢酶(EC 1.2.1.-)

481 G E 氧代戊二酸半醛脱氢酶(EC 1.2.1.-)

13 14 RXA00412 346 G S 结合ATP的ABC转运蛋白

15 16 RXA00483 387 G D 核苷二磷酸糖差向异构酶

17 18 RXA00511 23 A T 磷酸葡糖胺变位酶(EC 5.4.2.-)/乙酰葡糖胺磷酸变位酶(EC 5.4.2.3)/

磷酸甘露糖变位酶(EC 5.4.2.8)

19 20 RXA00526 58 D N 结合ATP的ABC转运蛋白

21 22 RXA00683 239 D N 烯醇丙酮酸磷酸合酶(EC 2.7.9.2)

23 24 RXA00684 421 S F 细胞色素P450 116(EC 1.14.-.-)

25 26 RXA00783 44 P S 琥珀酰-CoA合成酶β链(EC 6.2.1.5)

66 G E 琥珀酰-CoA合成酶β链(EC 6.2.1.5)

192 A T 琥珀酰-CoA合成酶β链(EC 6.2.1.5)

27 28 RXA00825 26 E K DTDP-葡萄糖4，6-脱水酶(EC 4.2.1.46)

29 30 RXA00868 379 E K IOLD蛋白

31 32 RXA00999 158 P S 6-磷酸葡糖酸脱氢酶，脱羧基(EC 1.1.1.44)

361 S F 6-磷酸葡糖酸脱氢酶，脱羧基(EC 1.1.1.44)

33 34 RXA01017 37 V I 推定的胞质蛋白

35 36 RXA01025 264 G S 3-磷酸甘油脱氢酶(NAD(P)+)(EC 1.1.1.94)

37 38 RXA01175 25 G S 磷酸-2-脱氢-3-脱氧庚糖酸醛缩酶(EC 4.1.2.15)

105 A V 磷酸-2-脱氢-3-脱氧庚糖酸醛缩酶(EC 4.1.2.15)

314 G D 磷酸-2-脱氢-3-脱氧庚糖酸醛缩酶(EC 4.1.2.15)

39 40 RXA01350 313 T I 苹果酸脱氢酶(EC 1.1.1.37)

41 42 RXA01392 120 D T 谷胱甘肽S-转移酶(EC 2.5.1.18)

125 R P 谷胱甘肽S-转移酶(EC 2.5.1.18)

126 F L 谷胱甘肽S-转移酶(EC 2.5.1.18)

128 D R 谷胱甘肽S-转移酶(EC 2.5.1.18)

188 R C 谷胱甘肽S-转移酶(EC 2.5.1.18)

43 44 RXA01436 342 D N 乙酸激酶(EC 2.7.2.1)

45 46 RXA01478 255 A T 葡糖淀粉酶G1和G2前体(EC 3.2.1.3)

47 48 RXA01554 124 G D 葡聚糖内-1，3-β-葡糖苷酶A1前体(EC 3.2.1.39)

486 G R 葡聚糖内-1，3-β-葡糖苷酶A1前体(EC 3.2.1.39)

49 50 RXA01562 59 V I 1-脱氧木酮糖-5-磷酸合酶

51 52 RXA01569 383 G D DTDP-4-脱氢鼠李糖3，5-差向异构酶(EC 5.1.3.13)/DTDP-4-脱氢鼠李

糖还原酶(EC 1.1.1.133)

53 54 RXA01693 102 A T 推定的胞质蛋白

55 56 RXA01814 53 S N 磷酸葡糖变位酶(EC 5.4.2.2)/磷酸甘露糖变位酶(EC 5.4.2.8)

57 58 RXA01882 109 L P 1-磷酸果糖激酶(EC 2.7.1.56)

59 60 RXA01887 142 G D 肌醇2-脱氢酶(EC 1.1.1.18)

61 62 RXA02056 1059 P L 2-氧代戊二酸脱氢酶E1组分(EC 1.2.4.2)

1121 A V 2-氧代戊二酸脱氢酶E1组分(EC 1.2.4.2)

63 64 RXA02063 137 G D 葡糖-1-磷酸腺苷酰转移酶(EC 2.7.7.27)

65 66 RXA02100 730 A T 糖元磷酸化酶(EC 2.4.1.1)

67 68 RXA02144 262 T I 甲代二氢醌-细胞色素C还原酶铁硫亚单位

69 70 RXA02149 213 A V 葡糖激酶(EC 2.7.1.2)

71 72 RXA02196 198 T I 磷酸甘醇酸磷酸酶(EC 3.1.3.18)

73 74 RXA02206 73 G D 氧化还原酶(EC 1.1.1.-)

114 A T 氧化还原酶(EC 1.1.1.-)

314 R C 氧化还原酶(EC 1.1.1.-)

75 76 RXA02399 348 A T 异柠檬酸裂合酶(EC 4.1.3.1)

77 78 RXA02404 51 E K 苹果酸合酶(EC 4.1.3.2)

79 80 RXA02414 140 G S 整合的膜蛋白(菱形家族)

81 82 RXA02434 525 P S 转运蛋白

83 84 RXA02440 41 T M 结合D-核糖的周质蛋白前体

85 86 RXA02474 7 E K (S，S)-丁烷-2，3-二醇脱氢酶(EC 1.1.1.76)

87 88 RXA02480 248 P S 细胞色素C氧化酶多肽I(EC 1.9.3.1)

89 90 RXA02560 138 G E 氧不敏感的NAD(P)H硝基还原酶(EC 1.-.-.-)/二氢蝶啶还原酶(EC

1.6.99.7)

192 P S 氧不敏感的NAD(P)H硝基还原酶(EC 1.-.-.-)/二氢蝶啶还原酶(EC

1.6.99.7)

91 92 RXA02591 377 P S 烯醇丙酮酸磷酸羧激酶[GTP](EC 4.1.1.32)

93 94 RXA02654 56 T I 葡萄糖1-脱氢酶(EC 1.1.1.47)

95 96 RXA02694 74 A V L-乳酸脱氢酶(EC 1.1.1.27)

97 98 RXA02735 27 P S 6-磷酸葡糖酸内酯酶(EC 3.1.1.31)

99 100 RXA02739 327 A T 转酮酶(EC 2.2.1.1)

101 102 RXA02740 313 G D 正铁血红素IX法呢基转移酶(EC 2.5.1.-)

103 104 RXA03030 162 P L 周质β-葡糖苷酶(EC 3.2.1.21)/β-木糖苷酶前体(EC 3.2.1.37)

105 106 RXA03083 351 A V 二氢硫辛酰胺脱氢酶(EC 1.8.1.4)

107 108 RXA03150 223 G V 醛脱氢酶(EC 1.2.1.3)

109 110 RXA03216 93 D N IOLE蛋白

111 112 RXA03388 129 A V UTP-葡糖-1-磷酸尿苷酰转移酶(EC 2.7.7.9)

113 114 RXA04088 39 E K 内-1，4-β-木聚糖酶A(EC 3.2.1.8)

115 116 RXA04279 480 R H 2-氧代戊二酸脱氢酶复合体(EC 2.3.1.61)中的二氢硫辛酰胺琥珀酰转

移酶组分(E2)

序列表

<110>巴斯福股份公司

<120>编码碳代谢和能量产生的蛋白质的基因

<130>O.Z.0050/52973

<160>116

<210>1

<211>1978

<212>DNA

<213>谷氨酸棒状杆菌(Corynebacterium glutamicum)

<220>

<221>CDS

<222>(101)..(1948)

<223>RXA00149

<400>1

ttttcgtagg taaacacagg tgaaggcttt acaagcttgt gaactcccta cacaaaagca 60

atccaatagc tatccataag caagagaaag taagtctacg ttg act gat ctc aca 115

Leu Thr Asp Leu Thr

1 5

aag act gcg gtg ccc gag gaa ctt tca gag aac ctc gaa act tgg tac 163

Lys Thr Ala Val Pro Glu Glu Leu Ser Glu Asn Leu Glu Thr Trp Tyr

10 15 20

aag gct gtg gcc ggt gtt ttc gcg cgc aca cag aaa aaa gac atc ggc 211

Lys Ala Val Ala Gly Val Phe Ala Arg Thr Gln Lys Lys Asp Ile Gly

25 30 35

gac att gcc gta gat gtg tgg aag aaa ctc atc gtc act aca ccg gat 259

Asp Ile Ala Val Asp Val Trp Lys Lys Leu Ile Val Thr Thr Pro Asp

40 45 50

ggt gtt gat atc aat ccg ctg tac acc aga gca gat gag tcc cag agg 307

Gly Val Asp Ile Asn Pro Leu Tyr Thr Arg Ala Asp Glu Ser Gln Arg

55 60 65

aaa ttc act gag gtt cct ggt gag ttt ccc ttc act agg gga acc act 355

Lys Phe Thr Glu Val Pro Gly Glu Phe Pro Phe Thr Arg Gly Thr Thr

70 75 80 85

gtt gat ggt gaa cgc gtt ggt tgg ggt gtt act gag act ttc gga cat 403

Val Asp Gly Glu Arg Val Gly Trp Gly Val Thr Glu Thr Phe Gly His

90 95 100

gac agc ccg aag aat atc aac gct gcg gtg ctg aat gct ctg aat tct 451

Asp Ser Pro Lys Asn Ile Asn Ala Ala Val Leu Asn Ala Leu Asn Ser

105 110 115

ggc acc acc aca ttg ggt ttt gag ttc tct gag gaa ttc acg gca gct 499

Gly Thr Thr Thr Leu Gly Phe Glu Phe Ser Glu Glu Phe Thr Ala Ala

120 125 130

gat ctt aaa gtt gct ctc gaa ggc gtg tat ctc aac atg gct ccg ttg 547

Asp Leu Lys Val Ala Leu Glu Gly Val Tyr Leu Asn Met Ala Pro Leu

135 140 145

ctg att cat gcg ggt gga tcc acg tca gag gtt gca gcg gcg ttg tat 595

Leu Ile His Ala Gly Gly Ser Thr Ser Glu Val Ala Ala Ala Leu Tyr

150 155 160 165

acg ttg gcg gag gaa gcc gga acg ttt ttt gct gcg ttg acc ttg ggt 643

Thr Leu Ala Glu Glu Ala Gly Thr Phe Phe Ala Ala Leu Thr Leu Gly

170 175 180

tct cgt cct ttg acg gcg cag gtt gat ggt tcg cac agt gac acc att 691

Ser Arg Pro Leu Thr Ala Gln Val Asp Gly Ser His Ser Asp Thr Ile

185 190 195

gaa gaa gca gtt cag ttg gca gtg aat gct tcc aag cgt gcg aat gtg 739

Glu Glu Ala Val Gln Leu Ala Val Asn Ala Ser Lys Arg Ala Asn Val

200 205 210

cgc gct atc ttg gtg gat ggt tcc agt ttt tcc aac cag ggc gcg tcg 787

Arg Ala Ile Leu Val Asp Gly Ser Ser Phe Ser Asn Gln Gly Ala Ser

215 220 225

gat gct caa gaa att ggt cta agt atc gcc gcc ggt gtg gat tat gtc 835

Asp Ala Gln Glu Ile Gly Leu Ser Ile Ala Ala Gly Val Asp Tyr Val

230 235 240 245

cgt cgc ttg gtc gat gca ggc ctt tcc acg gaa gct gca ctt aag cag 883

Arg Arg Leu Val Asp Ala Gly Leu Ser Thr Glu Ala Ala Leu Lys Gln

250 255 260

gtg gcg ttc cgt ttt gcg gtc acc gat gag cag ttc gcg cag att tct 931

Val Ala Phe Arg Phe Ala Val Thr Asp Glu Gln Phe Ala Gln Ile Ser

265 270 275

aag ctg cgt gtg gct cga cgt ctg tgg gcc agg gtg tgt gag gtg ctt 979

Lys Leu Arg Val Ala Arg Arg Leu Trp Ala Arg Val Cys Glu Val Leu

280 285 290

ggt ttt cca gag ctg gcc gta gca cca cag cat gcg gtg act gca cga 1027

Gly Phe Pro Glu Leu Ala Val Ala Pro Gln His Ala Val Thr Ala Arg

295 300 305

gcg atg ttt agc cag cgt gat ccg tgg gtg aat atg ctg cgc agt act 1075

Ala Met Phe Ser Gln Arg Asp Pro Trp Val Asn Met Leu Arg Ser Thr

310 315 320 325

gtt gca gct ttc gct gca ggc gtc ggt gga gca acc gat gtg gag gtt 1123

Val Ala Ala Phe Ala Ala Gly Val Gly Gly Ala Thr Asp Val Glu Val

330 335 340

cgt act ttt gat gat gcg atc cca gat gga gtt cct gga gtg tcg agg 1171

Arg Thr Phe Asp Asp Ala Ile Pro Asp Gly Val Pro Gly Val Ser Arg

345 350 355

aat ttc gct cac cgc atc gcg cgc aat act aat ttg ttg ttg cta gaa 1219

Asn Phe Ala His Arg Ile Ala Arg Asn Thr Asn Leu Leu Leu Leu Glu

360 365 370

gag tca cat ctt ggt cac gtg gtt gat cct gct ggt gga tca tat ttc 1267

Glu Ser His Leu Gly His Val Val Asp Pro Ala Gly Gly Ser Tyr Phe

375 380 385

gtg gag agc ttc acc gat gat cta gcg gag aag gcg tgg gct gtg ttc 1315

Val Glu Ser Phe Thr Asp Asp Leu Ala Glu Lys Ala Trp Ala Val Phe

390 395 400 405

agt ggc atc gaa gct gag ggc gga tac agt gca gct tgt gca tcc ggc 1363

Ser Gly Ile Glu Ala Glu Gly Gly Tyr Ser Ala Ala Cys Ala Ser Gly

410 415 420

acg gtg act gcc atg ctt gat cag acg tgg gag cag act cgc gct gat 1411

Thr Val Thr Ala Met Leu Asp Gln Thr Trp Glu Gln Thr Arg Ala Asp

425 430 435

gtg gcg tcg aga aag aag aag ctc act gga att aat gag ttc ccg aac 1459

Val Ala Ser Arg Lys Lys Lys Leu Thr Gly Ile Asn Glu Phe Pro Asn

440 445 450

ttg gcg gag tct ccg ctg cca gct gat cgt cgg gta gaa cct gca ggt 1507

Leu Ala Glu Ser Pro Leu Pro Ala Asp Arg Arg Val Glu Pro Ala Gly

455 460 465

gtg cgt cga tgg gca gcg gat ttt gaa gcg ctg cgc aat cgt tcg gat 1555

Val Arg Arg Trp Ala Ala Asp Phe Glu Ala Leu Arg Asn Arg Ser Asp

470 475 480 485

gct ttc ttg gaa aag aac ggc gcg agg cca cag atc acg atg att cct 1603

Ala Phe Leu Glu Lys Asn Gly Ala Arg Pro Gln Ile Thr Met Ile Pro

490 495 500

ctg gga ccg ttg tcc aag cac aat att cgc act ggt ttt act tcc aac 1651

Leu Gly Pro Leu Ser Lys His Asn Ile Arg Thr Gly Phe Thr Ser Asn

505 510 515

ctg ttg gct tcc ggt ggc att gaa gca atc aac ccg ggt caa ctt gtt 1699

Leu Leu Ala Ser Gly Gly Ile Glu Ala Ile Asn Pro Gly Gln Leu Val

520 525 530

ccc ggc act gac gct ttt gca gaa gct gca cag gcc gca ggc att gta 1747

Pro Gly Thr Asp Ala Phe Ala Glu Ala Ala Gln Ala Ala Gly Ile Val

535 540 545

gtg gtg tgt gga acg gac caa gag tat gcc gaa acg ggg gag gga gcc 1795

Val Val Cys Gly Thr Asp Gln Glu Tyr Ala Glu Thr Gly Glu Gly Ala

550 555 560 565

gtc gaa aag ctc cgc gaa gcg ggc gtt gag cgc atc ctg ctt gct ggc 1843

Val Glu Lys Leu Arg Glu Ala Gly Val Glu Arg Ile Leu Leu Ala Gly

570 575 580

gcg ccg aag agc ttt gag ggc agc gcg cat gcg ccc gat ggt tat ttg 1891

Ala Pro Lys Ser Phe Glu Gly Ser Ala His Ala Pro Asp Gly Tyr Leu

585 590 595

aac atg aca att gat gcc gcg gcg acg ctg gct gac ctg cta gat gct 1939

Asn Met Thr Ile Asp Ala Ala Ala Thr Leu Ala Asp Leu Leu Asp Ala

600 605 610

ttg gga gct taaatcatga cgtcgatccc taatttttca 1978

Leu Gly Ala

615

<210>2

<211>616

<212>PRT

<213>谷氨酸棒状杆菌

<400>2

Leu Thr Asp Leu Thr Lys Thr Ala Val Pro Glu Glu Leu Ser Glu Asn

1 5 10 15

Leu Glu Thr Trp Tyr Lys Ala Val Ala Gly Val Phe Ala Arg Thr Gln

20 25 30

Lys Lys Asp Ile Gly Asp Ile Ala Val Asp Val Trp Lys Lys Leu Ile

35 40 45

Val Thr Thr Pro Asp Gly Val Asp Ile Asn Pro Leu Tyr Thr Arg Ala

50 55 60

Asp Glu Ser Gln Arg Lys Phe Thr Glu Val Pro Gly Glu Phe Pro Phe

65 70 75 80

Thr Arg Gly Thr Thr Val Asp Gly Glu Arg Val Gly Trp Gly Val Thr

85 90 95

Glu Thr Phe Gly His Asp Ser Pro Lys Asn Ile Asn Ala Ala Val Leu

100 105 110

Asn Ala Leu Asn Ser Gly Thr Thr Thr Leu Gly Phe Glu Phe Ser Glu

115 120 125

Glu Phe Thr Ala Ala Asp Leu Lys Val Ala Leu Glu Gly Val Tyr Leu

130 135 140

Asn Met Ala Pro Leu Leu Ile His Ala Gly Gly Ser Thr Ser Glu Val

145 150 155 160

Ala Ala Ala Leu Tyr Thr Leu Ala Glu Glu Ala Gly Thr Phe Phe Ala

165 170 175

Ala Leu Thr Leu Gly Ser Arg Pro Leu Thr Ala Gln Val Asp Gly Ser

180 185 190

His Ser Asp Thr Ile Glu Glu Ala Val Gln Leu Ala Val Asn Ala Ser

195 200 205

Lys Arg Ala Asn Val Arg Ala Ile Leu Val Asp Gly Ser Ser Phe Ser

210 215 220

Asn Gln Gly Ala Ser Asp Ala Gln Glu Ile Gly Leu Ser Ile Ala Ala

225 230 235 240

Gly Val Asp Tyr Val Arg Arg Leu Val Asp Ala Gly Leu Ser Thr Glu

245 250 255

Ala Ala Leu Lys Gln Val Ala Phe Arg Phe Ala Val Thr Asp Glu Gln

260 265 270

Phe Ala Gln Ile Ser Lys Leu Arg Val Ala Arg Arg Leu Trp Ala Arg

275 280 285

Val Cys Glu Val Leu Gly Phe Pro Glu Leu Ala Val Ala Pro Gln His

290 295 300

Ala Val Thr Ala Arg Ala Met Phe Ser Gln Arg Asp Pro Trp Val Asn

305 310 315 320

Met Leu Arg Ser Thr Val Ala Ala Phe Ala Ala Gly Val Gly Gly Ala

325 330 335

Thr Asp Val Glu Val Arg Thr Phe Asp Asp Ala Ile Pro Asp Gly Val

340 345 350

Pro Gly Val Ser Arg Asn Phe Ala His Arg Ile Ala Arg Asn Thr Asn

355 360 365

Leu Leu Leu Leu Glu Glu Ser His Leu Gly His Val Val Asp Pro Ala

370 375 380

Gly Gly Ser Tyr Phe Val Glu Ser Phe Thr Asp Asp Leu Ala Glu Lys

385 390 395 400

Ala Trp Ala Val Phe Ser Gly Ile Glu Ala Glu Gly Gly Tyr Ser Ala

405 410 415

Ala Cys Ala Ser Gly Thr Val Thr Ala Met Leu Asp Gln Thr Trp Glu

420 425 430

Gln Thr Arg Ala Asp Val Ala Ser Arg Lys Lys Lys Leu Thr Gly Ile

435 440 445

Asn Glu Phe Pro Asn Leu Ala Glu Ser Pro Leu Pro Ala Asp Arg Arg

450 455 460

Val Glu Pro Ala Gly Val Arg Arg Trp Ala Ala Asp Phe Glu Ala Leu

465 470 475 480

Arg Asn Arg Ser Asp Ala Phe Leu Glu Lys Asn Gly Ala Arg Pro Gln

485 490 495

Ile Thr Met Ile Pro Leu Gly Pro Leu Ser Lys His Asn Ile Arg Thr

500 505 510

Gly Phe Thr Ser Asn Leu Leu Ala Ser Gly Gly Ile Glu Ala Ile Asn

515 520 525

Pro Gly Gln Leu Val Pro Gly Thr Asp Ala Phe Ala Glu Ala Ala Gln

530 535 540

Ala Ala Gly Ile Val Val Val Cys Gly Thr Asp Gln Glu Tyr Ala Glu

545 550 555 560

Thr Gly Glu Gly Ala Val Glu Lys Leu Arg Glu Ala Gly Val Glu Arg

565 570 575

Ile Leu Leu Ala Gly Ala Pro Lys Ser Phe Glu Gly Ser Ala His Ala

580 585 590

Pro Asp Gly Tyr Leu Asn Met Thr Ile Asp Ala Ala Ala Thr Leu Ala

595 600 605

Asp Leu Leu Asp Ala Leu Gly Ala

610 615

<210>3

<211>1306

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1276)

<223>RXA00196

<400>3

actgcgcttt cgcagtcatc atgcaggcat aacctgaaac ccatccgttt ggattgcccc 60

aaatgggtgt agtgggtgcg tttacccaac aagtgcaaga atg gga gtc gtg act 115

Met Gly Val Val Thr

1 5

aaa aag atc ctt att ttg gga agc act ggt tcg att gga act cag gcg 163

Lys Lys Ile Leu Ile Leu Gly Ser Thr Gly Ser Ile Gly Thr Gln Ala

10 15 20

ctg gac gtt att gct gat aat tca gac aag ttt gag gtg gtg ggt atc 211

Leu Asp Val Ile Ala Asp Asn Ser Asp Lys Phe Glu Val Val Gly Ile

25 30 35

gct gcg ggc ggt tct cag cca gac ctc gtt att tcg cag gcg cag cag 259

Ala Ala Gly Gly Ser Gln Pro Asp Leu Val Ile Ser Gln Ala Gln Gln

40 45 50

ttg ggg ctg gct gca gac aag gtt gcg gtt gct gat gca cag gct gcc 307

Leu Gly Leu Ala Ala Asp Lys Val Ala Val Ala Asp Ala Gln Ala Ala

55 60 65

gca gta att tcg aag gct ctc ggc ggc gag atc atc tct gga acc gat 355

Ala Val Ile Ser Lys Ala Leu Gly Gly Glu Ile Ile Ser Gly Thr Asp

70 75 80 85

gct gcg aag att ctg gtg gaa acc aca aag gcc gac act gtg ctt aat 403

Ala Ala Lys Ile Leu Val Glu Thr Thr Lys Ala Asp Thr Val Leu Asn

90 95 100

gct ctg gtt ggt tct ttg ggg ctt gcg gca acg ctg gcc act ctg gaa 451

Ala Leu Val Gly Ser Leu Gly Leu Ala Ala Thr Leu Ala Thr Leu Glu

105 110 115

tct ggt gcg cat ctt gcc ttg gct aac aaa gaa tcg ctg gtt gcc ggt 499

Ser Gly Ala His Leu Ala Leu Ala Asn Lys Glu Ser Leu Val Ala Gly

120 125 130

ggt gag ttt gtt acc tca aag gca aag ctg ggg cag atc att ccg gtc 547

Gly Glu Phe Val Thr Ser Lys Ala Lys Leu Gly Gln Ile Ile Pro Val

135 140 145

gat tcg gag cac tct gcc atg gcg cag tgt ttg cgt tcg ggt act cgt 595

Asp Ser Glu His Ser Ala Met Ala Gln Cys Leu Arg Ser Gly Thr Arg

150 155 160 165

gat gag gtt gcg cgg att gtg ctg aca gct tcg ggc gga cct ttc agg 643

Asp Glu Val Ala Arg Ile Val Leu Thr Ala Ser Gly Gly Pro Phe Arg

170 175 180

ggc tgg acc agg gag aag atg tgg gag gtg act ccc gag cag gca gca 691

Gly Trp Thr Arg Glu Lys Met Trp Glu Val Thr Pro Glu Gln Ala Ala

185 190 195

gcg cac cca acg tgg gcg atg ggg cag atg aac acg ttg aac tcc gcc 739

Ala His Pro Thr Trp Ala Met Gly Gln Met Asn Thr Leu Asn Ser Ala

200 205 210

acc ctt att aat aaa ggc ctc gaa ctc atc gag gcg acc ctg ctg ttt 787

Thr Leu Ile Asn Lys Gly Leu Glu Leu Ile Glu Ala Thr Leu Leu Phe

215 220 225

gaa acg gat gcg gat ctc att gat gtg acg gtg cat ccg cag tcg atc 835

Glu Thr Asp Ala Asp Leu Ile Asp Val Thr Val His Pro Gln Ser Ile

230 235 240 245

atc cac tcc atg atc acg ttt acg gat ggt gcg acc atc gcg cag gcg 883

Ile His Ser Met Ile Thr Phe Thr Asp Gly Ala Thr Ile Ala Gln Ala

250 255 260

tcg cca cca tcg atg aaa ctg ccg atc gcg ttg gcg ctt gat tgg cca 931

Ser Pro Pro Ser Met Lys Leu Pro Ile Ala Leu Ala Leu Asp Trp Pro

265 270 275

cat cgg gtg ccg aag gct cag ccg gcg ctg gat ttc acc gct gct cat 979

His Arg Val Pro Lys Ala Gln Pro Ala Leu Asp Phe Thr Ala Ala His

280 285 290

acc tgg gct ttt gag ccg gtg gat gat gcc gca ttc cct gcg gtg cag 1027

Thr Trp Ala Phe Glu Pro Val Asp Asp Ala Ala Phe Pro Ala Val Gln

295 300 305

ctg gct agg cac gtc gca aag caa aaa ggc acg tac ccc gcg gtg tat 1075

Leu Ala Arg His Val Ala Lys Gln Lys Gly Thr Tyr Pro Ala Val Tyr

310 315 320 325

aac gcc gcc aac gag gag gcg gct gag gcg ttt ttg cgc ggg cga atc 1123

Asn Ala Ala Asn Glu Glu Ala Ala Glu Ala Phe Leu Arg Gly Arg Ile

330 335 340

aag ttt ccg cag atc gtg gac gtg gtg gac gag gtc ctc caa gga gct 1171

Lys Phe Pro Gln Ile Val Asp Val Val Asp Glu Val Leu Gln Gly Ala

345 350 355

tct cag ttt gct ggt gta gca tca cac gtc gat gat att ttg gca acc 1219

Ser Gln Phe Ala Gly Val Ala Ser His Val Asp Asp Ile Leu Ala Thr

360 365 370

gaa tct gag gca cgc gcg cgt gcg aat gct ttg atc aac cgg ttg gca 1267

Glu Ser Glu Ala Arg Ala Arg Ala Asn Ala Leu Ile Asn Arg Leu Ala

375 380 385

acc aac ttg taagctaagg agcttccgcc tcgtggcagc 1306

Thr Asn Leu

390

<210>4

<211>392

<212>PRT

<213>谷氨酸棒状杆菌

<400>4

Met Gly Val Val Thr Lys Lys Ile Leu Ile Leu Gly Ser Thr Gly Ser

1 5 10 15

Ile Gly Thr Gln Ala Leu Asp Val Ile Ala Asp Asn Ser Asp Lys Phe

20 25 30

Glu Val Val Gly Ile Ala Ala Gly Gly Ser Gln Pro Asp Leu Val Ile

35 40 45

Ser Gln Ala Gln Gln Leu Gly Leu Ala Ala Asp Lys Val Ala Val Ala

50 55 60

Asp Ala Gln Ala Ala Ala Val Ile Ser Lys Ala Leu Gly Gly Glu Ile

65 70 75 80

Ile Ser Gly Thr Asp Ala Ala Lys Ile Leu Val Glu Thr Thr Lys Ala

85 90 95

Asp Thr Val Leu Asn Ala Leu Val Gly Ser Leu Gly Leu Ala Ala Thr

100 105 110

Leu Ala Thr Leu Glu Ser Gly Ala His Leu Ala Leu Ala Asn Lys Glu

115 120 125

Ser Leu Val Ala Gly Gly Glu Phe Val Thr Ser Lys Ala Lys Leu Gly

130 135 140

Gln Ile Ile Pro Val Asp Ser Glu His Ser Ala Met Ala Gln Cys Leu

145 150 155 160

Arg Ser Gly Thr Arg Asp Glu Val Ala Arg Ile Val Leu Thr Ala Ser

165 170 175

Gly Gly Pro Phe Arg Gly Trp Thr Arg Glu Lys Met Trp Glu Val Thr

180 185 190

Pro Glu Gln Ala Ala Ala His Pro Thr Trp Ala Met Gly Gln Met Asn

195 200 205

Thr Leu Asn Ser Ala Thr Leu Ile Asn Lys Gly Leu Glu Leu Ile Glu

210 215 220

Ala Thr Leu Leu Phe Glu Thr Asp Ala Asp Leu Ile Asp Val Thr Val

225 230 235 240

His Pro Gln Ser Ile Ile His Ser Met Ile Thr Phe Thr Asp Gly Ala

245 250 255

Thr Ile Ala Gln Ala Ser Pro Pro Ser Met Lys Leu Pro Ile Ala Leu

260 265 270

Ala Leu Asp Trp Pro His Arg Val Pro Lys Ala Gln Pro Ala Leu Asp

275 280 285

Phe Thr Ala Ala His Thr Trp Ala Phe Glu Pro Val Asp Asp Ala Ala

290 295 300

Phe Pro Ala Val Gln Leu Ala Arg His Val Ala Lys Gln Lys Gly Thr

305 310 315 320

Tyr Pro Ala Val Tyr Asn Ala Ala Asn Glu Glu Ala Ala Glu Ala Phe

325 330 335

Leu Arg Gly Arg Ile Lys Phe Pro Gln Ile Val Asp Val Val Asp Glu

340 345 350

Val Leu Gln Gly Ala Ser Gln Phe Ala Gly Val Ala Ser His Val Asp

355 360 365

Asp Ile Leu Ala Thr Glu Ser Glu Ala Arg Ala Arg Ala Asn Ala Leu

370 375 380

Ile Asn Arg Leu Ala Thr Asn Leu

385 390

<210>5

<211>1081

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1051)

<223>RXA00224

<400>5

gtgatgtcat tggtgcatcg ggtgctgctg aaaagattgc tgagtacctc gcttcagaga 60

acctcatcta gccactatct tcacaaagga gaacatttaa atg tct att tct tat 115

Met Ser Ile Ser Tyr

1 5

gtg ctg gtt gag cag cta gat ggc cgc cca gaa cca gtt acc ctt gaa 163

Val Leu Val Glu Gln Leu Asp Gly Arg Pro Glu Pro Val Thr Leu Glu

10 15 20

ttg atc act gct gct cgc gca ctc ggt gac gtc gtt gcc gtt gtc gtt 211

Leu Ile Thr Ala Ala Arg Ala Leu Gly Asp Val Val Ala Val Val Val

25 30 35

ggc gag cca ggt gcc ggc gta aac ctt gct gct gag ctc ggc aat tgg 259

Gly Glu Pro Gly Ala Gly Val Asn Leu Ala Ala Glu Leu Gly Asn Trp

40 45 50

ggt gca gca cag gtt gtt tcc gct gaa atc tct ggc gct tcc aac cgt 307

Gly Ala Ala Gln Val Val Ser Ala Glu Ile Ser Gly Ala Ser Asn Arg

55 60 65

ttg atc ttg cct gct gtt gat gcg ctg cac att ttg gct gcg aac aac 355

Leu Ile Leu Pro Ala Val Asp Ala Leu His Ile Leu Ala Ala Asn Asn

70 75 80 85

cca ggt cca att gtt atc gct gca act gca agc ggt aat gag atc gct 403

Pro Gly Pro Ile Val Ile Ala Ala Thr Ala Ser Gly Asn Glu Ile Ala

90 95 100

ggt cgt ttg gct gcc cgt ttg gct tct ggt gtg ctc acc gat gtc gtc 451

Gly Arg Leu Ala Ala Arg Leu Ala Ser Gly Val Leu Thr Asp Val Val

105 110 115

gga atc aat gcc gac cgc acc gca cag cag tcc att ttc ggc gac acc 499

Gly Ile Asn Ala Asp Arg Thr Ala Gln Gln Ser Ile Phe Gly Asp Thr

120 125 130

att cag gtg tcc gct gca gtt ggt ggc gct tca ccg ctg tac acc ctg 547

Ile Gln Val Ser Ala Ala Val Gly Gly Ala Ser Pro Leu Tyr Thr Leu

135 140 145

cgt cca ggt gcc ctt gat ggc gtg gcc gtt cct gca acc ggt gaa ttg 595

Arg Pro Gly Ala Leu Asp Gly Val Ala Val Pro Ala Thr Gly Glu Leu

150 155 160 165

gca acc att gag atc cca ggc gca acc gcc aag gat gtc acc atc acc 643

Ala Thr Ile Glu Ile Pro Gly Ala Thr Ala Lys Asp Val Thr Ile Thr

170 175 180

tcc ttc acg cca agc acc cag agc gat cgc cct gag ctg cca cag gca 691

Ser Phe Thr Pro Ser Thr Gln Ser Asp Arg Pro Glu Leu Pro Gln Ala

185 190 195

aag gtc gtt atc gca ggt gga cgt ggt gtc gga agc gaa gaa aac ttc 739

Lys Val Val Ile Ala Gly Gly Arg Gly Val Gly Ser Glu Glu Asn Phe

200 205 210

cgc agc atc gtt gaa cca ctg gca gat gca ttg ggc ggt gcc gtt ggc 787

Arg Ser Ile Val Glu Pro Leu Ala Asp Ala Leu Gly Gly Ala ValGly

215 220 225

gca acc cgc gac gcc gtt gat ctg ggc tac tac cca ggc gag tac cag 835

Ala Thr Arg Asp Ala Val Asp Leu Gly Tyr Tyr Pro Gly Glu Tyr Gln

230 235 240 245

gtt ggt cag acc ggt gtc acc gtg tcc cca gac ctc tac atc ggc ctc 883

Val Gly Gln Thr Gly Val Thr Val Ser Pro Asp Leu Tyr Ile Gly Leu

250 255 260

ggc att tcc ggt gca att cag cac act tct ggt atg cag acc gca aag 931

Gly Ile Ser Gly Ala Ile Gln His Thr Ser Gly Met Gln Thr Ala Lys

265 270 275

aag gtt att gtg atc aac aac gat gag gac gcg ccg atc ttc cag att 979

Lys Val Ile Val Ile Asn Asn Asp Glu Asp Ala Pro Ile Phe Gln Ile

280 285 290

gcg gac ctc ggt gtc gtt ggc gac ctc ttt gac atc gcc cct gcg ctc 1027

Ala Asp Leu Gly Val Val Gly Asp Leu Phe Asp Ile Ala Pro Ala Leu

295 300 305

atc gaa gag atc aac aag cgc aag taggagtttt gaacactttt tatctggacc 1081

Ile Glu Glu Ile Asn Lys Arg Lys

310 315

<210>6

<211>317

<212>PRT

<213>谷氨酸棒状杆菌

<400>6

Met Ser Ile Ser Tyr Val Leu Val Glu Gln Leu Asp Gly Arg Pro Glu

1 5 10 15

Pro Val Thr Leu Glu Leu Ile Thr Ala Ala Arg Ala Leu Gly Asp Val

20 25 30

Val Ala Val Val Val Gly Glu Pro Gly Ala Gly Val Asn Leu Ala Ala

35 40 45

Glu Leu Gly Asn Trp Gly Ala Ala Gln Val Val Ser Ala Glu Ile Ser

50 55 60

Gly Ala Ser Asn Arg Leu Ile Leu Pro Ala Val Asp Ala Leu His Ile

65 70 75 80

Leu Ala Ala Asn Asn Pro Gly Pro Ile Val Ile Ala Ala Thr Ala Ser

85 90 95

Gly Asn Glu Ile Ala Gly Arg Leu Ala Ala Arg Leu Ala Ser Gly Val

100 105 110

Leu Thr Asp Val Val Gly Ile Asn Ala Asp Arg Thr Ala Gln Gln Ser

115 120 125

Ile Phe Gly Asp Thr Ile Gln Val Ser Ala Ala Val Gly Gly Ala Ser

130 135 140

Pro Leu Tyr Thr Leu Arg Pro Gly Ala Leu Asp Gly Val Ala Val Pro

145 150 155 160

Ala Thr Gly Glu Leu Ala Thr Ile Glu Ile Pro Gly Ala Thr Ala Lys

165 170 175

Asp Val Thr Ile Thr Ser Phe Thr Pro Ser Thr Gln Ser Asp Arg Pro

180 185 190

Glu Leu Pro Gln Ala Lys Val Val Ile Ala Gly Gly Arg Gly Val Gly

195 200 205

Ser Glu Glu Asn Phe Arg Ser Ile Val Glu Pro Leu Ala Asp Ala Leu

210 215 220

Gly Gly Ala Val Gly Ala Thr Arg Asp Ala Val Asp Leu Gly Tyr Tyr

225 230 235 240

Pro Gly Glu Tyr Gln Val Gly Gln Thr Gly Val Thr Val Ser Pro Asp

245 250 255

Leu Tyr Ile Gly Leu Gly Ile Ser Gly Ala Ile Gln His Thr Ser Gly

260 265 270

Met Gln Thr Ala Lys Lys Val Ile Val Ile Asn Asn Asp Glu Asp Ala

275 280 285

Pro Ile Phe Gln Ile Ala Asp Leu Gly Val Val Gly Asp Leu Phe Asp

290 295 300

Ile Ala Pro Ala Leu Ile Glu Glu Ile Asn Lys Arg Lys

305 310 315

<210>7

<211>1405

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1375)

<223>RXA00235

<400>7

cgaaacaaga ttcgtgcaac aattgggtgt agacgtgatt gaagacattt gatcacgtga 60

ataattctag ttagctccca agttggcata ggaggccaca gtg gct gaa atc atg 115

Val Ala Glu Ile Met

1 5

cac gta ttc gct cgc gaa att ctc gac tcc cgc ggt aac cca acc gtc 163

His Val Phe Ala Arg Glu Ile Leu Asp Ser Arg Gly Asn Pro Thr Val

10 15 20

gag gca gag gtt ttc ctg gat gac ggt tcc cac ggt gtc gca ggt gtt 211

Glu Ala Glu Val Phe Leu Asp Asp Gly Ser His Gly Val Ala Gly Val

25 30 35

cca tcc ggc gca tcc acc ggc gtc cac gag gct cat gag ctg cgt gac 259

Pro Ser Gly Ala Ser Thr Gly Val His Glu Ala His Glu Leu Arg Asp

40 45 50

ggt ggc gat cgc tac ctg ggc aag ggc gtt ttg aag gca gtt gaa aac 307

Gly Gly Asp Arg Tyr Leu Gly Lys Gly Val Leu Lys Ala Val Glu Asn

55 60 65

gtc aac gaa gaa atc ggc gac gag ctc gct ggc cta gag gct gac gat 355

Val Asn Glu Glu Ile Gly Asp Glu Leu Ala Gly Leu Glu Ala Asp Asp

70 75 80 85

cag cgc ctc atc gac gaa gca atg atc aag ctt gat ggc acc gcc aac 403

Gln Arg Leu Ile Asp Glu Ala Met Ile Lys Leu Asp Gly Thr Ala Asn

90 95 100

aag tcc cgc ctg ggt gca aac gca atc ctt ggt gtt tcc atg gct gtt 451

Lys Ser Arg Leu Gly Ala Asn Ala Ile Leu Gly Val Ser Met Ala Val

105 110 115

gca aag gct gct gct gat tcc gca ggc ctc cca ctg ttc cgc tac atc 499

Ala Lys Ala Ala Ala Asp Ser Ala Gly Leu Pro Leu Phe Arg Tyr Ile

120 125 130

ggt gga cca aac gca cac gtt ctt cca gtt cca atg atg aac atc atc 547

Gly Gly Pro Asn Ala His Val Leu Pro Val Pro Met Met Asn Ile Ile

135 140 145

acc ggt ggc gct cac gct gac tcc ggt gtt gac gtt cag gaa ttc atg 595

Thr Gly Gly Ala His Ala Asp Ser Gly Val Asp Val Gln Glu Phe Met

150 155 160 165

atc gct cca atc ggt gca gag acc ttc tct gag gct ctc cgc aac ggc 643

Ile Ala Pro Ile Gly Ala Glu Thr Phe Ser Glu Ala Leu Arg Asn Gly

170 175 180

gcg gag gtc tac cac gca ctg aag tcc gtc atc aag gaa aag ggc ctg 691

Ala Glu Val Tyr His Ala Leu Lys Ser Val Ile Lys Glu Lys Gly Leu

185 190 195

tcc acc gga ctt ggc gat gag ggc ggc ttc gct cct tcc gtc ggc tcc 739

Ser Thr Gly Leu GIy Asp Glu Gly Gly Phe Ala Pro Ser Val Gly Ser

200 205 210

acc cgt gag gct ctt gac ctt atc gtt gag gca atc gag aag gct ggc 787

Thr Arg Glu Ala Leu Asp Leu Ile Val Glu Ala Ile Glu Lys Ala Gly

215 220 225

ttc acc cca ggc aag gac atc gct ctt gct ctg gac gtt gct tcc tct 835

Phe Thr Pro Gly Lys Asp Ile Ala Leu Ala Leu Asp Val Ala Ser Ser

230 235 240 245

gag ttc ttc aag gac ggc acc tac cac ttc gaa ggt ggc cag cac tcc 883

Glu Phe Phe Lys Asp Gly Thr Tyr His Phe Glu Gly Gly Gln His Ser

250 255 260

gca gct gag atg gca aac gtt tac gct gag ctc gtt gac gcg tac cca 931

Ala Ala Glu Met Ala Asn Val Tyr Ala Glu Leu Val Asp Ala Tyr Pro

265 270 275

atc gtc tcc atc gag gac cca ctg cag gaa gat gac tgg gag ggt tac 979

Ile Val Ser Ile Glu Asp Pro Leu Gln Glu Asp Asp Trp Glu Gly Tyr

280 285 290

acc aac ctc acc gca acc atc ggc gac aag gtt cag atc gtt ggc gac 1027

Thr Asn Leu Thr Ala Thr Ile Gly Asp Lys Val Gln Ile Val Gly Asp

295 300 305

gac ttc ttc gtc acc aac cct gag cgc ctg aag gag ggc atc gct aag 1075

Asp Phe Phe Val Thr Asn Pro Glu Arg Leu Lys Glu Gly Ile Ala Lys

310 315 320 325

aag gct gcc aac tcc atc ctg gtt aag gtg aac cag atc ggt acc ctc 1123

Lys Ala Ala Asn Ser Ile Leu Val Lys Val Asn Gln Ile Gly Thr Leu

330 335 340

acc gag acc ttc gac gct gtc gac atg gct cac cgc gca ggc tac acc 1171

Thr Glu Thr Phe Asp Ala Val Asp Met Ala His Arg Ala Gly Tyr Thr

345 350 355

tcc atg atg tcc cac cgt tcc ggt gag acc gag gac acc acc att gct 1219

Ser Met Met Ser His Arg Ser Gly Glu Thr Glu Asp Thr Thr Ile Ala

360 365 370

gac ctc gca gtt gca ctc aac tgt ggc cag atc aag act ggt gct cca 1267

Asp Leu Ala Val Ala Leu Asn Cys Gly Gln Ile Lys Thr Gly Ala Pro

375 380 385

gca cgt tcc gac cgt gtc gca aag tac aac cag ctt ctc cgc atc gag 1315

Ala Arg Ser Asp Arg Val Ala Lys Tyr Asn Gln Leu Leu Arg Ile Glu

390 395 400 405

cag ctg ctt ggc gac gcc ggc gtc tac gca ggt cgc agc gca ttc cca 1363

Gln Leu Leu Gly Asp Ala Gly Val Tyr Ala Gly Arg Ser Ala Phe Pro

410 415 420

cgc ttt cag ggc taaataaaag cgcttttcga cgcccggtaa 1405

Arg Phe Gln Gly

425

<210>8

<211>425

<212>PRT

<213>谷氨酸棒状杆菌

<400>8

Val Ala Glu Ile Met His Val Phe Ala Arg Glu Ile Leu Asp Ser Arg

1 5 10 15

Gly Asn Pro Thr Val Glu Ala Glu Val Phe Leu Asp Asp Gly Ser His

20 25 30

Gly Val Ala Gly Val Pro Ser Gly Ala Ser Thr Gly Val His Glu Ala

35 40 45

His Glu Leu Arg Asp Gly Gly Asp Arg Tyr Leu Gly Lys Gly Val Leu

50 55 60

Lys Ala Val Glu Asn Val Asn Glu Glu Ile Gly Asp Glu Leu Ala Gly

65 70 75 80

Leu Glu Ala Asp Asp Gln Arg Leu Ile Asp Glu Ala Met Ile Lys Leu

85 90 95

Asp Gly Thr Ala Asn Lys Ser Arg Leu Gly Ala Asn Ala Ile Leu Gly

100 105 110

Val Ser Met Ala Val Ala Lys Ala Ala Ala Asp Ser Ala Gly Leu Pro

115 120 125

Leu Phe Arg Tyr Ile Gly Gly Pro Asn Ala His Val Leu Pro Val Pro

130 135 140

Met Met Asn Ile Ile Thr Gly Gly Ala His Ala Asp Ser Gly Val Asp

145 150 155 160

Val Gln Glu Phe Met Ile Ala Pro Ile Gly Ala Glu Thr Phe Ser Glu

165 170 175

Ala Leu Arg Asn Gly Ala Glu Val Tyr His Ala Leu Lys Ser Val Ile

180 185 190

Lys Glu Lys Gly Leu Ser Thr Gly Leu Gly Asp Glu Gly Gly Phe Ala

195 200 205

Pro Ser Val Gly Ser Thr Arg Glu Ala Leu Asp Leu Ile Val Glu Ala

210 215 220

Ile Glu Lys Ala Gly Phe Thr Pro Gly Lys Asp Ile Ala Leu Ala Leu

225 230 235 240

Asp Val Ala Ser Ser Glu Phe Phe Lys Asp Gly Thr Tyr His Phe Glu

245 250 255

Gly Gly Gln His Ser Ala Ala Glu Met Ala Asn Val Tyr Ala Glu Leu

260 265 270

Val Asp Ala Tyr Pro Ile Val Ser Ile Glu Asp Pro Leu Gln Glu Asp

275 280 285

Asp Trp Glu Gly Tyr Thr Asn Leu Thr Ala Thr Ile Gly Asp Lys Val

290 295 300

Gln Ile Val Gly Asp Asp Phe Phe Val Thr Asn Pro Glu Arg Leu Lys

305 310 315 320

Glu Gly Ile Ala Lys Lys Ala Ala Asn Ser Ile Leu Val Lys Val Asn

325 330 335

Gln Ile Gly Thr Leu Thr Glu Thr Phe Asp Ala Val Asp Met Ala His

340 345 350

Arg Ala Gly Tyr Thr Ser Met Met Ser His Arg Ser Gly Glu Thr Glu

355 360 365

Asp Thr Thr Ile Ala Asp Leu Ala Val Ala Leu Asn Cys Gly Gln Ile

370 375 380

Lys Thr Gly Ala Pro Ala Arg Ser Asp Arg Val Ala Lys Tyr Asn Gln

385 390 395 400

Leu Leu Arg Ile Glu Gln Leu Leu Gly Asp Ala Gly Val Tyr Ala Gly

405 410 415

Arg Ser Ala Phe Pro Arg Phe Gln Gly

420 425

<210>9

<211>2974

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2944)

<223>RXA00296

<400>9

tttttgttgg catgtctgac attatcgcac aattcaccac agtaaccggt aacatgttac 60

tcaaagcccg cttggatcga atttcacaaa aggacccacc atg act cac acc atc 115

Met Thr His Thr Ile

1 5

aaa ttc aac aga ctc gac cca gaa gta ttt agc cag cat tct cgc gcg 163

Lys Phe Asn Arg Leu Asp Pro Glu Val Phe Ser Gln His Ser Arg Ala

10 15 20

aag ctg cgc acg gat atg aca acc cgt gca gca tat tct tct gat gca 211

Lys Leu Arg Thr Asp Met Thr Thr Arg Ala Ala Tyr Ser Ser Asp Ala

25 30 35

gga att ttt aga cgc gtc cct gca gct gta gct gaa cca gaa aat gtg 259

Gly Ile Phe Arg Arg Val Pro Ala Ala Val Ala Glu Pro Glu Asn Val

40 45 50

gaa caa att cgt gat gcc att gct gtt gcg gtg gca cga ggg tgg tct 307

Glu Gln Ile Arg Asp Ala Ile Ala Val Ala Val Ala Arg Gly Trp Ser

55 60 65

gtt gtt ggg cgc ggt gga gga agc tcg gtt gct gga aat gcg atc ggt 355

Val Val Gly Arg Gly Gly Gly Ser Ser Val Ala Gly Asn Ala Ile Gly

70 75 80 85

gaa ggt ttg atc atc gat acg tca cgc tat ttc aac cgc att tta gat 403

Glu Gly Leu Ile Ile Asp Thr Ser Arg Tyr Phe Asn Arg Ile Leu Asp

90 95 100

att gat cca gtt gca caa act gca gtt gtg gaa ccc ggt gtg gtg tgt 451

Ile Asp Pro Val Ala Gln Thr Ala Val Val Glu Pro Gly Val Val Cys

105 110 115

gat gcc ttg cgc gat gca gcc gca gaa ttc gga tta act tac ggc ccg 499

Asp Ala Leu Arg Asp Ala Ala Ala Glu Phe Gly Leu Thr Tyr Gly Pro

120 125 130

gat cct tcc acg cat tcc cgg tgc acg atc ggt ggc atg gtt gcc aac 547

Asp Pro Ser Thr His Ser Arg Cys Thr Ile Gly Gly Met Val Ala Asn

135 140 145

aat gcg tgt ggt tca cac tcg gtt gca ttc ggt aca gct gcg gaa aat 595

Asn Ala Cys Gly Ser His Ser Val Ala Phe Gly Thr Ala Ala Glu Asn

150 155 160 165

ctc gtg gat gtc acg ctc atg ctc agc gat ggc cga gaa gtc act gtg 643

Leu Val Asp Val Thr Leu Met Leu Ser Asp Gly Arg Glu Val Thr Val

170 175 180

aca aaa gat ggc tgc gat gat gct gag atc aat cag aag ctc acc gac 691

Thr Lys Asp Gly Cys Asp Asp Ala Glu Ile Asn Gln Lys Leu Thr Asp

185 190 195

tta gcg tcc aag aat cag gac ctt att agt aaa gaa ctg ggt cgt ttc 739

Leu Ala Ser Lys Asn Gln Asp Leu Ile Ser Lys Glu Leu Gly Arg Phe

200 205 210

cct cgc caa gtg tcg ggc tac ggt ttg cat tat ctt gcc cac gac atg 787

Pro Arg Gln Val Ser Gly Tyr Gly Leu His Tyr Leu Ala His Asp Met

215 220 225

gcc aaa gca atg gcg ggc acc gag gga acc att gga atc att act cgg 835

Ala Lys Ala Met Ala Gly Thr Glu Gly Thr Ile Gly Ile Ile Thr Arg

230 235 240 245

ttg acg gtg aag ttg gtt cca aca ccc aaa gtg aaa gcg ctt gct gtc 883

Leu Thr Val Lys Leu Val Pro Thr Pro Lys Val Lys Ala Leu Ala Val

250 255 260

ctg gct ttc gac acg gtt ttt gac gcc gcc cga gca gcc gcc aaa ttg 931

Leu Ala Phe Asp Thr Val Phe Asp Ala Ala Arg Ala Ala Ala Lys Leu

265 270 275

cga ctg cct ggg gta gca acc att gaa ggc atg ggc gga gat ctc ctc 979

Arg Leu Pro Gly Val Ala Thr Ile Glu Gly Met Gly Gly Asp Leu Leu

280 285 290

gct gcg ctg cgc agt aaa cag gga caa tca gaa gct ggg cag aat ctt 1027

Ala Ala Leu Arg Ser Lys Gln Gly Gln Ser Glu Ala Gly Gln Asn Leu

295 300 305

cca gga aac cgc atc ggc att gaa gcc ggc gga tgg ttg tac tgc gag 1075

Pro Gly Asn Arg Ile Gly Ile Glu Ala Gly Gly Trp Leu Tyr Cys Glu

310 315 320 325

aca gga agt gac acc ctg cag gcc gcg gta caa gcc gcc gag gaa gtc 1123

Thr Gly Ser Asp Thr Leu Gln Ala Ala Val Gln Ala Ala Glu Glu Val

330 335 340

gca acc gcc gtt gac acc att gat tac gtg gtc gtg tct gag cct tct 1171

Ala Thr Ala Val Asp Thr Ile Asp Tyr Val Val Val Ser Glu Pro Ser

345 350 355

gaa atg cgg gaa ttg tgg cgc atc cgt gaa tcc tcg gcg ggc att gtc 1219

Glu Met Arg Glu Leu Trp Arg Ile Arg Glu Ser Ser Ala Gly Ile Val

360 365 370

acg cgc tta gct gat ggt ggg gaa gcg tgg ccg aat tgg gaa gac tcg 1267

Thr Arg Leu Ala Asp Gly Gly Glu Ala Trp Pro Asn Trp Glu Asp Ser

375 380 385

gcg gtg cct cca gag aat tta gct gat tat ctc cgc gat ctt tat gcg 1315

Ala Val Pro Pro Glu Asn Leu Ala Asp Tyr Leu Arg Asp Leu Tyr Ala

390 395 400 405

ctg atg gat aag ttc gat tac cag ggt att cca ttt gga cac ttt gga 1363

Leu Met Asp Lys Phe Asp Tyr Gln Gly Ile Pro Phe Gly His Phe Gly

410 415 420

gaa ggc tgc gtc cac gtt cgc atc agt ttt gat ttc tct acc aag gaa 1411

Glu Gly Cys Val His Val Arg Ile Ser Phe Asp Phe Ser Thr Lys Glu

425 430 435

ggc ctg aag aaa ttc gag gcg ttc atg aat gaa gcc tcc acc ttg gtg 1459

Gly Leu Lys Lys Phe Glu Ala Phe Met Asn Glu Ala Ser Thr Leu Val

440 445 450

gcg tct tat ggt ggc agc ctc tcg ggc gag cat gga gac ggt cgc gcc 1507

Ala Ser Tyr Gly Gly Ser Leu Ser Gly Glu His Gly Asp Gly Arg Ala

455 460 465

cgc tca tcc ttc ctt gac cgc atg tat tca gca gaa atg cgt gca ctc 1555

Arg Ser Ser Phe Leu Asp Arg Met Tyr Ser Ala Glu Met Arg Ala Leu

470 475 480 485

ttc gaa gaa ttc aag ctg att ttc gat ccc cag cgc atc ttc aat ccg 1603

Phe Glu Glu Phe Lys Leu Ile Phe Asp Pro Gln Arg Ile Phe Asn Pro

490 495 500

gga gtg ttg gtc tgg gca gat cct gtc atg caa gga ctt cgc atg gac 1651

Gly Val Leu Val Trp Ala Asp Pro Val Met Gln Gly Leu Arg Met Asp

505 510 515

ccg ggc cag cgc gcc ctc gac atc acg ccc gta cac aaa ttc tct aaa 1699

Pro Gly Gln Arg Ala Leu Asp Ile Thr Pro Val His Lys Phe Ser Lys

520 525 530

gac aaa ggt tcc atg atc aac gcg gtg aat cgc tgc gtg ggt gta tcc 1747

Asp Lys Gly Ser Met Ile Asn Ala Val Asn Arg Cys Val Gly Val Ser

535 540 545

gca tgc cgc tca gaa tcc gac gcg atg tgc ccg tcc ttc caa atc acc 1795

Ala Cys Arg Ser Glu Ser Asp Ala Met Cys Pro Ser Phe Gln Ile Thr

550 555 560 565

ggc gac gaa gta cat tcc acc aga ggc cgc gcc cgc ttg ctc tct gag 1843

Gly Asp Glu Val His Ser Thr Arg Gly Arg Ala Arg Leu Leu Ser Glu

570 575 580

atg ttc cgc ggt gaa tcc atc gcc gac ggc tac cgc agc gaa gaa gtc 1891

Met Phe Arg Gly Glu Ser Ile Ala Asp Gly Tyr Arg Ser Glu Glu Val

585 590 595

aat gaa gcc ctt gac ctg tgc ctt tcc tgc aaa gca tgc gca tcg gaa 1939

Asn Glu Ala Leu Asp Leu Cys Leu Ser Cys Lys Ala Cys Ala Ser Glu

600 605 610

tgt cca gtc aac gtc gac atg tcc acc tac aaa gcc gaa ttc ctg gac 1987

Cys Pro Val Asn Val Asp Met Ser Thr Tyr Lys Ala Glu Phe Leu Asp

615 620 625

aaa cac tac gcc ggc cga ctt cgc ccc atg gcc cat tac gtc atg ggc 2035

Lys His Tyr Ala Gly Arg Leu Arg Pro Met Ala His Tyr Val Met Gly

630 635 640 645

tgg ctg ccg ctt ctg gga cac gtt gcc cat aaa ata ccg ctt ctt cct 2083

Trp Leu Pro Leu Leu Gly His Val Ala His Lys Ile Pro Leu Leu Pro

650 655 660

acg ctt atc gac gcc acc atg cag tca gca ctc acc gcc cca gtg gtg 2131

Thr Leu Ile Asp Ala Thr Met Gln Ser Ala Leu Thr Ala Pro Val Val

665 670 675

cgc aag gtc ggc ggg ctc gct gat cgc ccg ttg att tcc ttc gcc cac 2179

Arg Lys Val Gly Gly Leu Ala Asp Arg Pro Leu Ile Ser Phe Ala His

680 685 690

cgc tcg ctg cgc aag tac aag ccg aag aaa aac tca ggt gaa acg gtg 2227

Arg Ser Leu Arg Lys Tyr Lys Pro Lys Lys Asn Ser Gly Glu Thr Val

695 700 705

gtg ctg tgg ccc gat tcc ttc aac acc aac ctc gac acc gga cca gct 2275

Val Leu Trp Pro Asp Ser Phe Asn Thr Asn Leu Asp Thr Gly Pro Ala

710 715 720 725

cac gca gcg atc aaa act ctt gaa gcc ctc ggt tac aac gtg gtc atc 2323

His Ala Ala Ile Lys Thr Leu Glu Ala Leu Gly Tyr Asn Val Val Ile

730 735 740

cca gat ggc ttc gtc tgc tgt gga ctc acc tgg cat tcc acc ggc caa 2371

Pro Asp Gly Phe Val Cys Cys Gly Leu Thr Trp His Ser Thr Gly Gln

745 750 755

ttg agc atg aca aag aaa gtc cta gaa caa acg gcg aaa gtg atg aaa 2419

Leu Ser Met Thr Lys Lys Val Leu Glu Gln Thr Ala Lys Val Met Lys

760 765 770

ccc tac ctg gac caa ggt cta aca gtc gtt ggt ttg gaa cct tcg tgc 2467

Pro Tyr Leu Asp Gln Gly Leu Thr Val Val Gly Leu Glu Pro Ser Cys

775 780 785

acc gtc atg ctt caa gat gag gca aca gaa ctc tcc gat aac cct gat 2515

Thr Val Met Leu Gln Asp Glu Ala Thr Glu Leu Ser Asp Asn Pro Asp

790 795 800 805

ctg gca cgc ctt gca gca ctg acc aaa cca ttc gct gag gtc atc gca 2563

Leu Ala Arg Leu Ala Ala Leu Thr Lys Pro Phe Ala Glu Val Ile Ala

810 815 820

cca aag atc acc gag cta gtc gag tct gga agc ctc cag cta aca gaa 2611

Pro Lys Ile Thr Glu Leu Val Glu Ser Gly Ser Leu Gln Leu Thr Glu

825 830 835

tca act gcg ctt acc cag gtg cac tgc cac gag cgt tcg cta ggc gac 2659

Ser Thr Ala Leu Thr Gln Val His Cys His Glu Arg Ser Leu Gly Asp

840 845 850

cca caa caa tcg gca ctc gtt ctt gaa gct ttg ggt gta aaa gat gaa 2707

Pro Gln Gln Ser Ala Leu Val Leu Glu Ala Leu Gly ValLys Asp Glu

855 860 865

caa att gcc act ggt tgt tgc ggg ctt gcc gga aac tgg ggc ttt gaa 2755

Gln Ile Ala Thr Gly Cys Cys Gly Leu Ala Gly Asn Trp Gly Phe Glu

870 875 880 885

aaa gac cac gct gaa atg tcc ttc gca ctt ggt gaa cga gag ctg ttc 2803

Lys Asp His Ala Glu Met Ser Phe Ala Leu Gly Glu Arg Glu Leu Phe

890 895 900

ccc aag gtc aga aaa gca gaa gga cat gtg att gct gac ggt ttc tcc 2851

Pro Lys Val Arg Lys Ala Glu Gly His Val Ile Ala Asp Gly Phe Ser

905 910 915

tgc cgc acc cag atc gaa caa ggc acc gga aaa caa gca acg cac ctt 2899

Cys Arg Thr Gln Ile Glu Gln Gly Thr Gly Lys Gln Ala Thr His Leu

920 925 930

gca gag gtg gtc tta agc atc ttg gag caa aac aac atg gca caa 2944

Ala Glu Val Val Leu Ser Ile Leu Glu Gln Asn Asn Met Ala Gln

935 940 945

taacgatcat gcaacaggtg ctcatgggtt 2974

<210>10

<211>948

<212>PRT

<213>谷氨酸棒状杆菌

<400>10

Met Thr His Thr Ile Lys Phe Asn Arg Leu Asp Pro Glu Val Phe Ser

1 5 10 15

Gln His Ser Arg Ala Lys Leu Arg Thr Asp Met Thr Thr Arg Ala Ala

20 25 30

Tyr Ser Ser Asp Ala Gly Ile Phe Arg Arg Val Pro Ala Ala Val Ala

35 40 45

Glu Pro Glu Asn Val Glu Gln Ile Arg Asp Ala Ile Ala Val Ala Val

50 55 60

Ala Arg Gly Trp Ser Val Val Gly Arg Gly Gly Gly Ser Ser Val Ala

65 70 75 80

Gly Asn Ala Ile Gly Glu Gly Leu Ile Ile Asp Thr Ser Arg Tyr Phe

85 90 95

Asn Arg Ile Leu Asp Ile Asp Pro Val Ala Gln Thr Ala Val Val Glu

100 105 110

Pro Gly Val Val Cys Asp Ala Leu Arg Asp Ala Ala Ala Glu Phe Gly

115 120 125

Leu Thr Tyr Gly Pro Asp Pro Ser Thr His Ser Arg Cys Thr Ile Gly

130 135 140

Gly Met Val Ala Asn Asn Ala Cys Gly Ser His Ser Val Ala Phe Gly

145 150 155 160

Thr Ala Ala Glu Asn Leu Val Asp Val Thr Leu Met Leu Ser Asp Gly

165 170 175

Arg Glu Val Thr Val Thr Lys Asp Gly Cys Asp Asp Ala Glu Ile Asn

180 185 190

Gln Lys Leu Thr Asp Leu Ala Ser Lys Asn Gln Asp Leu Ile Ser Lys

195 200 205

Glu Leu Gly Arg Phe Pro Arg Gln Val Ser Gly Tyr Gly Leu His Tyr

210 215 220

Leu Ala His Asp Met Ala Lys Ala Met Ala Gly Thr Glu Gly Thr Ile

225 230 235 240

Gly Ile Ile Thr Arg Leu Thr Val Lys Leu Val Pro Thr Pro Lys Val

245 250 255

Lys Ala Leu Ala Val Leu Ala Phe Asp Thr Val Phe Asp Ala Ala Arg

260 265 270

Ala Ala Ala Lys Leu Arg Leu Pro Gly Val Ala Thr Ile Glu Gly Met

275 280 285

Gly Gly Asp Leu Leu Ala Ala Leu Arg Ser Lys Gln Gly Gln Ser Glu

290 295 300

Ala Gly Gln Asn Leu Pro Gly Asn Arg Ile Gly Ile Glu Ala Gly Gly

305 310 315 320

Trp Leu Tyr Cys Glu Thr Gly Ser Asp Thr Leu Gln Ala Ala Val Gln

325 330 335

Ala Ala Glu Glu Val Ala Thr Ala Val Asp Thr Ile Asp Tyr Val Val

340 345 350

Val Ser Glu Pro Ser Glu Met Arg Glu Leu Trp Arg Ile Arg Glu Ser

355 360 365

Ser Ala Gly Ile Val Thr Arg Leu Ala Asp Gly Gly Glu Ala Trp Pro

370 375 380

Asn Trp Glu Asp Ser Ala Val Pro Pro Glu Asn Leu Ala Asp Tyr Leu

385 390 395 400

Arg Asp Leu Tyr Ala Leu Met Asp Lys Phe Asp Tyr Gln Gly Ile Pro

405 410 415

Phe Gly His Phe Gly Glu Gly Cys Val His Val Arg Ile Ser Phe Asp

420 425 430

Phe Ser Thr Lys Glu Gly Leu Lys Lys Phe Glu Ala Phe Met Asn Glu

435 440 445

Ala Ser Thr Leu Val Ala Ser Tyr Gly Gly Ser Leu Ser Gly Glu His

450 455 460

Gly Asp Gly Arg Ala Arg Ser Ser Phe Leu Asp Arg Met Tyr Ser Ala

465 470 475 480

Glu Met Arg Ala Leu Phe Glu Glu Phe Lys Leu Ile Phe Asp Pro Gln

485 490 495

Arg Ile Phe Asn Pro Gly Val Leu Val Trp Ala Asp Pro Val Met Gln

500 505 510

Gly Leu Arg Met Asp Pro Gly Gln Arg Ala Leu Asp Ile Thr Pro Val

515 520 525

His Lys Phe Ser Lys Asp Lys Gly Ser Met Ile Asn Ala Val Asn Arg

530 535 540

Cys Val Gly Val Ser Ala Cys Arg Ser Glu Ser Asp Ala Met Cys Pro

545 550 555 560

Ser Phe Gln Ile Thr Gly Asp Glu Val His Ser Thr Arg Gly Arg Ala

565 570 575

Arg Leu Leu Ser Glu Met Phe Arg Gly Glu Ser Ile Ala Asp Gly Tyr

580 585 590

Arg Ser Glu Glu Val Asn Glu Ala Leu Asp Leu Cys Leu Ser Cys Lys

595 600 605

Ala Cys Ala Ser Glu Cys Pro Val Asn Val Asp Met Ser Thr Tyr Lys

610 615 620

Ala Glu Phe Leu Asp Lys His Tyr Ala Gly Arg Leu Arg Pro Met Ala

625 630 635 640

His Tyr Val Met Gly Trp Leu Pro Leu Leu Gly His Val Ala His Lys

645 650 655

Ile Pro Leu Leu Pro Thr Leu Ile Asp Ala Thr Met Gln Ser Ala Leu

660 665 670

Thr Ala Pro Val Val Arg Lys Val Gly Gly Leu Ala Asp Arg Pro Leu

675 680 685

Ile Ser Phe Ala His Arg Ser Leu Arg Lys Tyr Lys Pro Lys Lys Asn

690 695 700

Ser Gly Glu Thr Val Val Leu Trp Pro Asp Ser Phe Asn Thr Asn Leu

705 710 715 720

Asp Thr Gly Pro Ala His Ala Ala Ile Lys Thr Leu Glu Ala Leu Gly

725 730 735

Tyr Asn Val Val Ile Pro Asp Gly Phe Val Cys Cys Gly Leu Thr Trp

740 745 750

His Ser Thr Gly Gln Leu Ser Met Thr Lys Lys Val Leu Glu Gln Thr

755 760 765

Ala Lys Val Met Lys Pro Tyr Leu Asp Gln Gly Leu Thr Val Val Gly

770 775 780

Leu Glu Pro Ser Cys Thr Val Met Leu Gln Asp Glu Ala Thr Glu Leu

785 790 795 800

Ser Asp Asn Pro Asp Leu Ala Arg Leu Ala Ala Leu Thr Lys Pro Phe

805 810 815

Ala Glu Val Ile Ala Pro Lys Ile Thr Glu Leu Val Glu Ser Gly Ser

820 825 830

Leu Gln Leu Thr Glu Ser Thr Ala Leu Thr Gln Val His Cys His Glu

835 840 845

Arg Ser Leu Gly Asp Pro Gln Gln Ser Ala Leu Val Leu Glu Ala Leu

850 855 860

Gly Val Lys Asp Glu Gln Ile Ala Thr Gly Cys Cys Gly Leu Ala Gly

865 870 875 880

Asn Trp Gly Phe Glu Lys Asp His Ala Glu Met Ser Phe Ala Leu Gly

885 890 895

Glu Arg Glu Leu Phe Pro Lys Val Arg Lys Ala Glu Gly His Val Ile

900 905 910

Ala Asp Gly Phe Ser Cys Arg Thr Gln Ile Glu Gln Gly Thr Gly Lys

915 920 925

Gln Ala Thr His Leu Ala Glu Val Val Leu Ser Ile Leu Glu Gln Asn

930 935 940

Asn Met Ala Gln

945

<210>11

<211>1690

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1660)

<223>RXA00389

<400>11

ccaccactgc gtaacctttc cgagcaagat atcgcggacc tgtcggattt gcttgccacc 60

tctggcgcag gttcctaccg ccttcagttg aggtgaaagc atg atc acc gca acc 115

Met Ile Thr Ala Thr

1 5

gca ctg cat ggg tgt tca ctg att gat ggc gag tgg gtc gct gga aaa 163

Ala Leu His Gly Cys Ser Leu Ile Asp Gly Glu Trp Val Ala Gly Lys

10 15 20

aat ggt gag att aca gga ttc gat ccg cgc acc aat gcg agt ctg aac 211

Asn Gly Glu Ile Thr Gly Phe Asp Pro Arg Thr Asn Ala Ser Leu Asn

25 30 35

cct tcc tac tct tta gca aac agc gca cag ctg cgc gcc gcc aca aca 259

Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln Leu Arg Ala Ala Thr Thr

40 45 50

tcg gcg aag cga gct ttt gaa agc tac cga ctc act act cca gag gtt 307

Ser Ala Lys Arg Ala Phe Glu Ser Tyr Arg Leu Thr Thr Pro Glu Val

55 60 65

aga gca gat ttc ctg gat tcc atc gct gac aac atc gat gcg cta tcc 355

Arg Ala Asp Phe Leu Asp Ser Ile Ala Asp Asn Ile Asp Ala Leu Ser

70 75 80 85

ggc gag atc gtg caa cgg gcg agc ctg gag aca ggt ttg gga act acc 403

Gly Glu Ile Val Gln Arg Ala Ser Leu Glu Thr Gly Leu Gly Thr Thr

90 95 100

cga ctc aca ggc gaa gta gcc cgc acc agc aac cag ctc cgc ctg ttt 451

Arg Leu Thr Gly Glu Val Ala Arg Thr Ser Asn Gln Leu Arg Leu Phe

105 110 115

gca gaa acc gtg aga agc gga cag ttc cac cga gta cgc att gaa cga 499

Ala Glu Thr Val Arg Ser Gly Gln Phe His Arg Val Arg Ile Glu Arg

120 125 130

gga ccg cgg att gat ctt cgc cag cgt cag gtt ccg ttg gga cca gtc 547

Gly Pro Arg Ile Asp Leu Arg Gln Arg Gln Val Pro Leu Gly Pro Val

135 140 145

gcg gta ttc ggg gca agc aac ttc ccc gtc gct ttc tct act gct ggt 595

Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala Phe Ser Thr Ala Gly

150 155 160 165

ggc gat aca gca tca gcg ttg gct gca ggc tgc cct gtg gtt ttt aag 643

Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys Pro Val Val Phe Lys

170 175 180

gcg cat aat gcg cac cct gga aca gct gag ctc gtc ggg caa gcg gtg 691

Ala His Asn Ala His Pro Gly Thr Ala Glu Leu Val Gly Gln Ala Val

185 190 195

cgg gga gcc gtc gaa aag cat gag ttt gat gct ggt gtg ttt aac ctt 739

Arg Gly Ala Val Glu Lys His Glu Phe Asp Ala Gly Val Phe Asn Leu

200 205 210

gtc tac ggc cgt ggc gtg gaa att ggc cag gag ctg gct gcg gat ccg 787

Val Tyr Gly Arg Gly Val Glu Ile Gly Gln Glu Leu Ala Ala Asp Pro

215 220 225

aat atc acg gca atc ggt ttt acc ggt tca cgc cag ggt ggt ttg gca 835

Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg Gln Gly Gly Leu Ala

230 235 240 245

ctg tca cag act gcg ttt agc cgc cca gtt ccc gtt cca gtc ttt gca 883

Leu Ser Gln Thr Ala Phe Ser Arg Pro Val Pro Val Pro Val Phe Ala

250 255 260

gaa atg agt gcc acc aac cct gtg ttc gtc ttc ccc ggc gcg ctg gcg 931

Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe Pro Gly Ala Leu Ala

265 270 275

gat ttg gat gca tcg agt tcc ttg gct gag gcg ttt acc gct tcc gtc 979

Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala Phe Thr Ala Ser Val

280 285 290

acc ggc agt tcc ggg caa ttg tgc acc aag cct ggc ctc gtt ttc atc 1027

Thr Gly Ser Ser Gly Gln Leu Cys Thr Lys Pro Gly Leu Val Phe Ile

295 300 305

ccg cgc ggt gtt gtt ggt gat gct ttt gtg gcg ctc gta gca gcc aaa 1075

Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala Leu ValAla Ala Lys

310 315 320 325

ttt aaa gaa acc acg ggt caa acg atg ctc acg caa ggc atc gct cag 1123

Phe Lys Glu Thr Thr Gly Gln Thr Met Leu Thr Gln Gly Ile Ala Gln

330 335 340

gca tgg cag cgc gga gtc gac aac ctt gca gca cag cca agt gta aaa 1171

Ala Trp Gln Arg Gly Val Asp Asn Leu Ala Ala Gln Pro Ser Val Lys

345 350 355

atc ctc gcc caa ggc acc ccc gga gat gga gag aac gcg ccg ggc ccg 1219

Ile Leu Ala Gln Gly Thr Pro Gly Asp Gly Glu Asn Ala Pro Gly Pro

360 365 370

gtg gtg ttt gaa agt gat gtg cag gcg ttg cta aat aat gtg gtg ttg 1267

Val Val Phe Glu Ser Asp Val Gln Ala Leu Leu Asn Asn Val Val Leu

375 380 385

cag gaa gaa atc ttc ggt gcg gca tcg ctg gtg gtg cgt tat gat tcc 1315

Gln Glu Glu Ile Phe Gly Ala Ala Ser Leu Val Val Arg Tyr Asp Ser

390 395 400 405

ccg gat caa ctc cac caa gta gcc aat tca ctc gag gga caa tta aca 1363

Pro Asp Gln Leu His Gln Val Ala Asn Ser Leu Glu Gly Gln Leu Thr

410 415 420

gcc acg atc cac gca tcc cag gat gat ttc cag gaa gtc tcg aaa ctt 1411

Ala Thr Ile His Ala Ser Gln Asp Asp Phe Gln Glu Val Ser Lys Leu

425 430 435

atc ccc ctc ttg gag gat ctc gcg ggc cgt gtt ctt tac ggc ggc tgg 1459

Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val Leu Tyr Gly Gly Trp

440 445 450

cca acg ggt gtg gaa gtt ggg cac acg gtt atc cat gga ggc cct tat 1507

Pro Thr Gly Val Glu Val Gly His Thr Val Ile His Gly Gly Pro Tyr

455 460 465

ccg gcg acc tca aat gcg cag tcg aca agt gtt gga acc ctg gca atc 1555

Pro Ala Thr Ser Asn Ala Gln Ser Thr Ser Val Gly Thr Leu Ala Ile

470 475 480 485

gag aga ttt atg cgc ccg gtt tct tat caa act ttc ccg gct gag ctg 1603

Glu Arg Phe Met Arg Pro Val Ser Tyr Gln Thr Phe Pro Ala Glu Leu

490 495 500

ctt cca gat cca gtt tct gag gcg aat aaa tgg gct gta cct cgg gaa 1651

Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp Ala Val Pro Arg Glu

505 510 515

ata gac cgt taatagctgg tctttacatt tgccagaaag 1690

Ile Asp Arg

520

<210>12

<211>520

<212>PRT

<213>谷氨酸棒状杆菌

<400>12

Met Ile Thr Ala Thr Ala Leu His Gly Cys Ser Leu Ile Asp Gly Glu

1 5 10 15

Trp Val Ala Gly Lys Asn Gly Glu Ile Thr Gly Phe Asp Pro Arg Thr

20 25 30

Asn Ala Ser Leu Asn Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln Leu

35 40 45

Arg Ala Ala Thr Thr Ser Ala Lys Arg Ala Phe Glu Ser Tyr Arg Leu

50 55 60

Thr Thr Pro Glu Val Arg Ala Asp Phe Leu Asp Ser Ile Ala Asp Asn

65 70 75 80

Ile Asp Ala Leu Ser Gly Glu Ile Val Gln Arg Ala Ser Leu Glu Thr

85 90 95

Gly Leu Gly Thr Thr Arg Leu Thr Gly Glu Val Ala Arg Thr Ser Asn

100 105 110

Gln Leu Arg Leu Phe Ala Glu Thr Val Arg Ser Gly Gln Phe His Arg

115 120 125

Val Arg Ile Glu Arg Gly Pro Arg Ile Asp Leu Arg Gln Arg Gln Val

130 135 140

Pro Leu Gly Pro Val Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala

145 150 155 160

Phe Ser Thr Ala Gly Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys

165 170 175

Pro Val Val Phe Lys Ala His Asn Ala His Pro Gly Thr Ala Glu Leu

180 185 190

Val Gly Gln Ala Val Arg Gly Ala Val Glu Lys His Glu Phe Asp Ala

195 200 205

Gly Val Phe Asn Leu Val Tyr Gly Arg Gly Val Glu Ile Gly Gln Glu

210 215 220

Leu Ala Ala Asp Pro Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg

225 230 235 240

Gln Gly Gly Leu Ala Leu Ser Gln Thr Ala Phe Ser Arg Pro Val Pro

245 250 255

Val Pro Val Phe Ala Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe

260 265 270

Pro Gly Ala Leu Ala Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala

275 280 285

Phe Thr Ala Ser Val Thr Gly Ser Ser Gly Gln Leu Cys Thr Lys Pro

290 295 300

Gly Leu Val Phe Ile Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala

305 310 315 320

Leu Val Ala Ala Lys Phe Lys Glu Thr Thr Gly Gln Thr Met Leu Thr

325 330 335

Gln Gly Ile Ala Gln Ala Trp Gln Arg Gly Val Asp Asn Leu Ala Ala

340 345 350

Gln Pro Ser Val Lys Ile Leu Ala Gln Gly Thr Pro Gly Asp Gly Glu

355 360 365

Asn Ala Pro Gly Pro Val Val Phe Glu Ser Asp Val Gln Ala Leu Leu

370 375 380

Asn Asn Val Val Leu Gln Glu Glu Ile Phe Gly Ala Ala Ser Leu Val

385 390 395 400

Val Arg Tyr Asp Ser Pro Asp Gln Leu His Gln Val Ala Asn Ser Leu

405 410 415

Glu Gly Gln Leu Thr Ala Thr Ile His Ala Ser Gln Asp Asp Phe Gln

420 425 430

Glu Val Ser Lys Leu Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val

435 440 445

Leu Tyr Gly Gly Trp Pro Thr Gly Val Glu Val Gly His Thr Val Ile

450 455 460

His Gly Gly Pro Tyr Pro Ala Thr Ser Asn Ala Gln Ser Thr Ser Val

465 470 475 480

Gly Thr Leu Ala Ile Glu Arg Phe Met Arg Pro Val Ser Tyr Gln Thr

485 490 495

Phe Pro Ala Glu Leu Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp

500 505 510

Ala Val Pro Arg Glu Ile Asp Arg

515 520

<210>13

<211>1261

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1231)

<223>RXA00412

<400>13

caatcacagc ctgaaaatca gactgtgaac ttcaaacgcc ccgcttggaa tcttttgacg 60

aacaccacgt cgcgtacgct tcctcggggc gttaaactat ttg tct tcc agc ttt 115

Leu Ser Ser Ser Phe

1 5

tgt ccc ccg act ttt gta cga atc gag gac acc gtc gtg tca cac acc 163

Cys Pro Pro Thr Phe Val Arg Ile Glu Asp Thr Val Val Ser His Thr

10 15 20

gcg tcc aca ccg acg cca gag gaa tac tcc gcg cag caa ccc agc acc 211

Ala Ser Thr Pro Thr Pro Glu Glu Tyr Ser Ala Gln Gln Pro Ser Thr

25 30 35

cag ggc act cgc gtt gag ttc cgc ggc ata acc aaa gtc ttt agc aac 259

Gln Gly Thr Arg Val Glu Phe Arg Gly Ile Thr Lys Val Phe Ser Asn

40 45 50

aat aaa tct gct aaa acc acc gcg ctt gat aat gtc act ctc acc gta 307

Asn Lys Ser Ala Lys Thr Thr Ala Leu Asp Asn Val Thr Leu Thr Val

55 60 65

gaa ccc ggt gag gta atc ggc atc atc ggt tac tct ggc gcc ggc aag 355

Glu Pro Gly Glu Val Ile Gly Ile Ile Gly Tyr Ser Gly Ala Gly Lys

70 75 80 85

tcc act ctt gtc cgc ctc atc aat ggc ctt gac tcc ccc acg agc ggt 403

Ser Thr Leu Val Arg Leu Ile Asn Gly Leu Asp Ser Pro Thr Ser Gly

90 95 100

tcg ttg ctg ctc aac ggc acc gac atc gtc gga atg ccc gag tct aag 451

Ser Leu Leu Leu Asn Gly Thr Asp Ile Val Gly Met Pro Glu Ser Lys

105 110 115

ctg cgt aaa ctg cgc agt aat atc ggc atg att ttc cag cag ttc aac 499

Leu Arg Lys Leu Arg Ser Asn Ile Gly Met Ile Phe Gln Gln Phe Asn

120 125 130

ctg ttc cag tcg cgt act gcg gct gga aat gtg gag tac ccg ctg gaa 547

Leu Phe Gln Ser Arg Thr Ala Ala Gly Asn Val Glu Tyr Pro Leu Glu

135 140 145

gtt gcc aag atg gac aag gca gct cgt aaa gct cgc gtg caa gaa atg 595

Val Ala Lys Met Asp Lys Ala Ala Arg Lys Ala Arg Val Gln Glu Met

150 155 160 165

ctc gag ttc gtc ggc ctg ggc gac aaa ggc aaa aac tac ccc gag cag 643

Leu Glu Phe Val Gly Leu Gly Asp Lys Gly Lys Asn Tyr Pro Glu Gln

170 175 180

ctg tcg ggc ggc cag aag cag cgc gtc ggc att gcc cgt gca ctg gcc 691

Leu Ser Gly Gly Gln Lys Gln Arg Val Gly Ile Ala Arg Ala Leu Ala

185 190 195

acc aat cca acg ctt ttg ctt gcc gac gaa gcc acc tcc gct ttg gac 739

Thr Asn Pro Thr Leu Leu Leu Ala Asp Glu Ala Thr Ser Ala Leu Asp

200 205 210

cca gaa acc acc cat gaa gtt ctg gag ctg ctg cgc aag gta aac cgc 787

Pro Glu Thr Thr His Glu Val Leu Glu Leu Leu Arg Lys Val Asn Arg

215 220 225

gaa ctg ggc atc acc atc gtt gtg atc acc cac gaa atg gaa gtt gtg 835

Glu Leu Gly Ile Thr Ile Val Val Ile Thr His Glu Met Glu Val Val

230 235 240 245

cgt tcc atc gca gac aag gtt gct gtg atg gaa tcc ggc aaa gtt gtg 883

Arg Ser Ile Ala Asp Lys Val Ala Val Met Glu Ser Gly Lys Val Val

250 255 260

gaa tac ggc agc gtc tac gag gtg ttc tcc aat cca caa aca cag gtt 931

Glu Tyr Gly Ser Val Tyr Glu Val Phe Ser Asn Pro Gln Thr Gln Val

265 270 275

gct caa aag ttc gtg gcc acc gcg ctg cgt aac acc cca gac caa gtg 979

Ala Gln Lys Phe Val Ala Thr Ala Leu Arg Asn Thr Pro Asp Gln Val

280 285 290

gaa tcg gaa gat ctg ctt agc cat gag gga cgt ctg ttc acc att gat 1027

Glu Ser Glu Asp Leu Leu Ser His Glu Gly Arg Leu Phe Thr Ile Asp

295 300 305

ctg act gaa acg tcc ggc ttc ttt gca gca acc gct cgt gct gcc gaa 1075

Leu Thr Glu Thr Ser Gly Phe Phe Ala Ala Thr Ala Arg Ala Ala Glu

310 315 320 325

caa ggt gct ttt gtc aac atc gtt cac ggt ggc gtg acc acc ttg caa 1123

Gln Gly Ala Phe Val Asn Ile Val His Gly Gly Val Thr Thr Leu Gln

330 335 340

cgc caa tca ttt ggc aaa atg act gtt cga ctc acc ggc aac acc gct 1171

Arg Gln Ser Phe Gly Lys Met Thr Val Arg Leu Thr Gly Asn Thr Ala

345 350 355

gcg att gaa gag ttc tat caa acc ttg acc aag acc acg acc atc aag 1219

Ala Ile Glu Glu Phe Tyr Gln Thr Leu Thr Lys Thr Thr Thr Ile Lys

360 365 370

gag atc acc cga tgaacgagat gatcctcgca gctgactgga 1261

Glu Ile Thr Arg

375

<210>14

<211>377

<212>PRT

<213>谷氨酸棒状杆菌

<400>14

Leu Ser Ser Ser Phe Cys Pro Pro Thr Phe Val Arg Ile Glu Asp Thr

1 5 10 15

Val Val Ser His Thr Ala Ser Thr Pro Thr Pro Glu Glu Tyr Ser Ala

20 25 30

Gln Gln Pro Ser Thr Gln Gly Thr Arg Val Glu Phe Arg Gly Ile Thr

35 40 45

Lys Val Phe Ser Asn Asn Lys Ser Ala Lys Thr Thr Ala Leu Asp Asn

50 55 60

Val Thr Leu Thr Val Glu Pro Gly Glu Val Ile Gly Ile Ile Gly Tyr

65 70 75 80

Ser Gly Ala Gly Lys Ser Thr Leu Val Arg Leu Ile Asn Gly Leu Asp

85 90 95

Ser Pro Thr Ser Gly Ser Leu Leu Leu Asn Gly Thr Asp Ile Val Gly

100 105 110

Met Pro Glu Ser Lys Leu Arg Lys Leu Arg Ser Asn Ile Gly Met Ile

115 120 125

Phe Gln Gln Phe Asn Leu Phe Gln Ser Arg Thr Ala Ala Gly Asn Val

130 135 140

Glu Tyr Pro Leu Glu Val Ala Lys Met Asp Lys Ala Ala Arg Lys Ala

145 150 155 160

Arg Val Gln Glu Met Leu Glu Phe Val Gly Leu Gly Asp Lys Gly Lys

165 170 175

Asn Tyr Pro Glu Gln Leu Ser Gly Gly Gln Lys Gln Arg Val Gly Ile

180 185 190

Ala Arg Ala Leu Ala Thr Asn Pro Thr Leu Leu Leu Ala Asp Glu Ala

195 200 205

Thr Ser Ala Leu Asp Pro Glu Thr Thr His Glu Val Leu Glu Leu Leu

210 215 220

Arg Lys Val Asn Arg Glu Leu Gly Ile Thr Ile Val Val Ile Thr His

225 230 235 240

Glu Met Glu Val Val Arg Ser Ile Ala Asp Lys Val Ala Val Met Glu

245 250 255

Ser Gly Lys Val Val Glu Tyr Gly Ser Val Tyr Glu Val Phe Ser Asn

260 265 270

Pro Gln Thr Gln Val Ala Gln Lys Phe Val Ala Thr Ala Leu Arg Asn

275 280 285

Thr Pro Asp Gln Val Glu Ser Glu Asp Leu Leu Ser His Glu Gly Arg

290 295 300

Leu Phe Thr Ile Asp Leu Thr Glu Thr Ser Gly Phe Phe Ala Ala Thr

305 310 315 320

Ala Arg Ala Ala Glu Gln Gly Ala Phe Val Asn Ile Val His Gly Gly

325 330 335

Val Thr Thr Leu Gln Arg Gln Ser Phe Gly Lys Met Thr Val Arg Leu

340 345 350

Thr Gly Asn Thr Ala Ala Ile Glu Glu Phe Tyr Gln Thr Leu Thr Lys

355 360 365

Thr Thr Thr Ile Lys Glu Ile Thr Arg

370 375

<210>15

<211>1669

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1639)

<223>RXA00483

<400>15

ttattacttc taaaagtata gtagatagtt gtggatgggt gaagaatttc atagaaatcg 60

cactcgattc actaaagacc caagagtaaa atcccaggat ttg ctt ata ctt gcg 115

Leu Leu Ile Leu Ala

1 5

ctc atg gat aat caa ctt cgt ccc act ttg cat tat caa gct caa aac 163

Leu Met Asp Asn Gln Leu Arg Pro Thr Leu His Tyr Gln Ala Gln Asn

10 15 20

ccg cac cgg cga gtg ctg gtc acc ggt gcg aca ggc tac att ggc ggc 211

Pro His Arg Arg Val Leu Val Thr Gly Ala Thr Gly Tyr Ile Gly Gly

25 30 35

agg ttg att act gag tta ctt gct gcc ggt ttc caa gtt cgg gcc acc 259

Arg Leu Ile Thr Glu Leu Leu Ala Ala Gly Phe Gln Val Arg Ala Thr

40 45 50

tcg agg aaa aaa aca agt ctt cag cgc ttt gac tgg tac gag gac gtc 307

Ser Arg Lys Lys Thr Ser Leu Gln Arg Phe Asp Trp Tyr Glu Asp Val

55 60 65

gag gca gtg gaa gcg gat ctg act gac gcg act gag tta gat acg tta 355

Glu Ala Val Glu Ala Asp Leu Thr Asp Ala Thr Glu Leu Asp Thr Leu

70 75 80 85

ttt aag gat gta gac gtt gtt tac tat cta gtg cat tcc atg gga ggt 403

Phe Lys Asp Val Asp Val Val Tyr Tyr Leu Val His Ser Met Gly Gly

90 95 100

aag aat gtt gat ttt gaa gag caa gag caa cgc act gct gaa aat gta 451

Lys Asn Val Asp Phe Glu Glu Gln Glu Gln Arg Thr Ala Glu Asn Val

105 110 115

att caa gct gct gat caa gcc ggg ata aaa cag att gtc tac ctt tcc 499

Ile Gln Ala Ala Asp Gln Ala Gly Ile Lys Gln Ile Val Tyr Leu Ser

120 125 130

ggc tta cac ccg cgt aat cga aaa ata gaa gaa cta tct aag cac atg 547

Gly Leu His Pro Arg Asn Arg Lys Ile Glu Glu Leu Ser Lys His Met

135 140 145

cgc tca cgg gaa aag gtc gcc cag att ttg ctg gca ggc cag aca cca 595

Arg Ser Arg Glu Lys Val Ala Gln Ile Leu Leu Ala Gly Gln Thr Pro

150 155 160 165

gct tta att tta agg gct gcc aca att att ggt tcc ggc tct gca tca 643

Ala Leu Ile Leu Arg Ala Ala Thr Ile Ile Gly Ser Gly Ser Ala Ser

170 175 180

ttt gaa ata atc cgt cat ctc acg gag cgt ttg cct aga atg ata gcg 691

Phe Glu Ile Ile Arg His Leu Thr Glu Arg Leu Pro Arg Met Ile Ala

185 190 195

cct cag tgg att act aat cag att gag cct tta gca ata cgg gat gtt 739

Pro Gln Trp Ile Thr Asn Gln Ile Glu Pro Leu Ala Ile Arg Asp Val

200 205 210

ttg cat tac cta atc tcg gcg gct gat tta aag gat cca gtc aac cgc 787

Leu His Tyr Leu Ile Ser Ala Ala Asp Leu Lys Asp Pro Val Asn Arg

215 220 225

tcc tgc gat att ggg tgt gga aag tcg tat gaa ttt gcg gat cta ttg 835

Ser Cys Asp Ile Gly Cys Gly Lys Ser Tyr Glu Phe Ala Asp Leu Leu

230 235 240 245

cgt atc tat gcc gat gtt cgg gga ctg aaa cgt cat gta aat tcc gta 883

Arg Ile Tyr Ala Asp Val Arg Gly Leu Lys Arg His Val Asn Ser Val

250 255 260

cct ctc aat ttg ccc atg gac aag cta tcc ggt ctt tgg att agt cta 931

Pro Leu Asn Leu Pro Met Asp Lys Leu Ser Gly Leu Trp Ile Ser Leu

265 270 275

gtg aca cct gtt cca ttt caa ttg tct ttc cct tta gct caa tca atg 979

Val Thr Pro Val Pro Phe Gln Leu Ser Phe Pro Leu Ala Gln Ser Met

280 285 290

gct gag gat gcc gtc act gaa gag cac agc att aaa gat att att tca 1027

Ala Glu Asp Ala Val Thr Glu Glu His Ser Ile Lys Asp Ile Ile Ser

295 300 305

gat cca ccc gat ggt ttt att gag tat cgg gaa gca gtg gag ctg gca 1075

Asp Pro Pro Asp Gly Phe Ile Glu Tyr Arg Glu Ala Val Glu Leu Ala

310 315 320 325

tta gct gca gaa ttt gat cgt gga gtt cca acg tca tgg gat cga agc 1123

Leu Ala Ala Glu Phe Asp Arg Gly Val Pro Thr Ser Trp Asp Arg Ser

330 335 340

tgg act gta caa caa ccg tgg gct ggc cag cct acc gat cca gag tgg 1171

Trp Thr Val Gln Gln Pro Trp Ala Gly Gln Pro Thr Asp Pro Glu Trp

345 350 355

gcg ggc aaa gct gta tat gaa gac gtc cgc aca gaa gat act gat ctc 1219

Ala Gly Lys Ala Val Tyr Glu Asp Val Arg Thr Glu Asp Thr Asp Leu

360 365 370

cga gca gcg cag gtc tgg ccg atc att gaa ggt ttg ggt ggc gtg aac 1267

Arg Ala Ala Gln Val Trp Pro Ile Ile Glu Gly Leu Gly Gly Val Asn

375 380 385

ggc tgg tat tct gca cca ctg cta tgg cga ttg cgg ggt atc gct gac 1315

Gly Trp Tyr Ser Ala Pro Leu Leu Trp Arg Leu Arg Gly Ile Ala Asp

390 395 400 405

aga ctc atc ggc ggt cca ggt ttg ggc gga cgg cgg gat ccc cgt cat 1363

Arg Leu Ile Gly Gly Pro Gly Leu Gly Gly Arg Arg Asp Pro Arg His

410 415 420

ttg aaa ctt ggg gat cgc att gat tgg tgg cgg gtt act gag atc gat 1411

Leu Lys Leu Gly Asp Arg Ile Asp Trp Trp Arg Val Thr Glu Ile Asp

425 430 435

cca cca cat aga tta gtg ctc acc gca gag atg aaa gta gat ggt ggc 1459

Pro Pro His Arg Leu Val Leu Thr Ala Glu Met Lys Val Asp Gly Gly

440 445 450

gct tgg ctg atc ctg gaa gtt gcg gac aag gaa aat ggc gga tgt act 1507

Ala Trp Leu Ile Leu Glu Val Ala Asp Lys Glu Asn Gly Gly Cys Thr

455 460 465

tat acc cag cgc gca ata ttt gag ccg aag ggt ttg ccc ggt tat ctc 1555

Tyr Thr Gln Arg Ala Ile Phe Glu Pro Lys Gly Leu Pro Gly Tyr Leu

470 475 480 485

tat tgg tgg gtt gtt tca ccg ttc cat gcg att att ttt cct tat atg 1603

Tyr Trp Trp Val Val Ser Pro Phe His Ala Ile Ile Phe Pro Tyr Met

490 495 500

cgt tcg aat att tta aaa gct gcg cgt aaa ctc act taatcgcaga 1649

Arg Ser Asn Ile Leu Lys Ala Ala Arg Lys Leu Thr

505 510

gtaggcgtct aaagcaattt 1669

<210>16

<211>513

<212>PRT

<213>谷氨酸棒状杆菌

<400>16

Leu Leu Ile Leu Ala Leu Met Asp Asn Gln Leu Arg Pro Thr Leu His

1 5 10 15

Tyr Gln Ala Gln Asn Pro His Arg Arg Val Leu Val Thr Gly Ala Thr

20 25 30

Gly Tyr Ile Gly Gly Arg Leu Ile Thr Glu Leu Leu Ala Ala Gly Phe

35 40 45

Gln Val Arg Ala Thr Ser Arg Lys Lys Thr Ser Leu Gln Arg Phe Asp

50 55 60

Trp Tyr Glu Asp Val Glu Ala Val Glu Ala Asp Leu Thr Asp Ala Thr

65 70 75 80

Glu Leu Asp Thr Leu Phe Lys Asp Val Asp Val Val Tyr Tyr Leu Val

85 90 95

His Ser Met Gly Gly Lys Asn Val Asp Phe Glu Glu Gln Glu Gln Arg

100 105 110

Thr Ala Glu Asn Val Ile Gln Ala Ala Asp Gln Ala Gly Ile Lys Gln

115 120 125

Ile Val Tyr Leu Ser Gly Leu His Pro Arg Asn Arg Lys Ile Glu Glu

130 135 140

Leu Ser Lys His Met Arg Ser Arg Glu Lys Val Ala Gln Ile Leu Leu

145 150 155 160

Ala Gly Gln Thr Pro Ala Leu Ile Leu Arg Ala Ala Thr Ile Ile Gly

165 170 175

Ser Gly Ser Ala Ser Phe Glu Ile Ile Arg His Leu Thr Glu Arg Leu

180 185 190

Pro Arg Met Ile Ala Pro Gln Trp Ile Thr Asn Gln Ile Glu Pro Leu

195 200 205

Ala Ile Arg Asp Val Leu His Tyr Leu Ile Ser Ala Ala Asp Leu Lys

210 215 220

Asp Pro Val Asn Arg Ser Cys Asp Ile Gly Cys Gly Lys Ser Tyr Glu

225 230 235 240

Phe Ala Asp Leu Leu Arg Ile Tyr Ala Asp Val Arg Gly Leu Lys Arg

245 250 255

His Val Asn Ser Val Pro Leu Asn Leu Pro Met Asp Lys Leu Ser Gly

260 265 270

Leu Trp Ile Ser Leu Val Thr Pro Val Pro Phe Gln Leu Ser Phe Pro

275 280 285

Leu Ala Gln Ser Met Ala Glu Asp Ala Val Thr Glu Glu His Ser Ile

290 295 300

Lys Asp Ile Ile Ser Asp Pro Pro Asp Gly Phe Ile Glu Tyr Arg Glu

305 310 315 320

Ala Val Glu Leu Ala Leu Ala Ala Glu Phe Asp Arg Gly Val Pro Thr

325 330 335

Ser Trp Asp Arg Ser Trp Thr Val Gln Gln Pro Trp Ala Gly Gln Pro

340 345 350

Thr Asp Pro Glu Trp Ala Gly Lys Ala Val Tyr Glu Asp Val Arg Thr

355 360 365

Glu Asp Thr Asp Leu Arg Ala Ala Gln Val Trp Pro Ile Ile Glu Gly

370 375 380

Leu Gly Gly Val Asn Gly Trp Tyr Ser Ala Pro Leu Leu Trp Arg Leu

385 390 395 400

Arg Gly Ile Ala Asp Arg Leu Ile Gly Gly Pro Gly Leu Gly Gly Arg

405 410 415

Arg Asp Pro Arg His Leu Lys Leu Gly Asp Arg Ile Asp Trp Trp Arg

420 425 430

Val Thr Glu Ile Asp Pro Pro His Arg Leu Val Leu Thr Ala Glu Met

435 440 445

Lys Val Asp Gly Gly Ala Trp Leu Ile Leu Glu Val Ala Asp Lys Glu

450 455 460

Asn Gly Gly Cys Thr Tyr Thr Gln Arg Ala Ile Phe Glu Pro Lys Gly

465 470 475 480

Leu Pro Gly Tyr Leu Tyr Trp Trp Val Val Ser Pro Phe His Ala Ile

485 490 495

Ile Phe Pro Tyr Met Arg Ser Asn Ile Leu Lys Ala Ala Arg Lys Leu

500 505 510

Thr

<210>17

<211>1498

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1468)

<223>RXA00511

<400>17

gggttttccg cattcttggg gtttcttgtg acttagggac ttgggtttac acagctcaac 60

cgaagaaaaa ttgttcaata gagttttgaa caatgcgatc ttg gcc agg ccg tgc 115

Leu Ala Arg Pro Cys

1 5

ata att aat cgc atg act cga cta ttt gga act gat ggc gtc cgc gga 163

Ile Ile Asn Arg Met Thr Arg Leu Phe Gly Thr Asp Gly Val Arg Gly

10 15 20

cta gcc aat gaa gta ctc acc gca cct ttg gcc ttg aag ctg ggg gcc 211

Leu Ala Asn Glu Val Leu Thr Ala Pro Leu Ala Leu Lys Leu Gly Ala

25 30 35

gct gca gct cac gta ctt acc gca gag aaa cgg gta gat gga cgt cgc 259

Ala Ala Ala His Val Leu Thr Ala Glu Lys Arg Val Asp Gly Arg Arg

40 45 50

ccg gtt gcg atc gtt ggt agg gat cct cga gtc tct gga gag atg ctc 307

Pro Val Ala Ile Val Gly Arg Asp Pro Arg Val Ser Gly Glu Met Leu

55 60 65

gcg gca gca ctt tca gca ggc atg gcc agc cag ggt gtt gat gtc att 355

Ala Ala Ala Leu Ser Ala Gly Met Ala Ser Gln Gly Val Asp Val Ile

70 75 80 85

cgt gtt ggt gtc atc cca acc cct gct gtt gca ttc ctc acc gat gat 403

Arg Val Gly Val Ile Pro Thr Pro Ala Val Ala Phe Leu Thr Asp Asp

90 95 100

tat ggc gct gac atg ggc gtg atg att tct gca tcc cac aac cca atg 451

Tyr Gly Ala Asp Met Gly Val Met Ile Ser Ala Ser His Asn Pro Met

105 110 115

ccg gac aac gga atc aag ttc ttc tct gca ggt gga cac aag ctt cca 499

Pro Asp Asn Gly Ile Lys Phe Phe Ser Ala Gly Gly His Lys Leu Pro

120 125 130

gac cat gtg gaa gac gag att gag cgt gtt atg gac agc ttg cca gca 547

Asp His Val Glu Asp Glu Ile Glu Arg Val Met Asp Ser Leu Pro Ala

135 140 145

gaa ggc cca aca ggg cat gga gtt ggc cgt gtc atc gaa gaa gca acc 595

Glu Gly Pro Thr Gly His Gly Val Gly Arg Val Ile Glu Glu Ala Thr

150 155 160 165

gat gca cag gac cgt tac cta gag cac ctg aag gaa gct gtt cct acg 643

Asp Ala Gln Asp Arg Tyr Leu Glu His Leu Lys Glu Ala Val Pro Thr

170 175 180

tca ctt gaa ggc atc aag att gtt gtg gat gca gcc aat ggt gcg gca 691

Ser Leu Glu Gly Ile Lys Ile Val Val Asp Ala Ala Asn Gly Ala Ala

185 190 195

agt gtt gta gct cca acg gct tat gag gct gcg ggt gca act gta att 739

Ser Val Val Ala Pro Thr Ala Tyr Glu Ala Ala Gly Ala Thr Val Ile

200 205 210

gct att cat aac aag cca gac tca tac aac atc aac atg gac tgc ggt 787

Ala Ile His Asn Lys Pro Asp Ser Tyr Asn Ile Asn Met Asp Cys Gly

215 220 225

tcc acc cac att gat cag gtg cag gcg gca gtc ctg aag cac ggt gct 835

Ser Thr His Ile Asp Gln Val Gln Ala Ala Val Leu Lys His Gly Ala

230 235 240 245

gac ctt gga ctc gcg cat gac ggt gat gct gac cgt tgt ttg gct gtg 883

Asp Leu Gly Leu Ala His Asp Gly Asp Ala Asp Arg Cys Leu Ala Val

250 255 260

gac aag gat ggc aac ctt gtt gat ggt gac caa atc atg gcg ctg tta 931

Asp Lys Asp Gly Asn Leu Val Asp Gly Asp Gln Ile Met Ala Leu Leu

265 270 275

gcc att gcg atg aaa gaa aac ggc gag ctg cgc aag aac acc ctc gtg 979

Ala Ile Ala Met Lys Glu Asn Gly Glu Leu Arg Lys Asn Thr Leu Val

280 285 290

ggc act gtc atg agc aac ctg gga ttg aag att gct atg gat gaa gcc 1027

Gly Thr Val Met Ser Asn Leu Gly Leu Lys Ile Ala Met Asp Glu Ala

295 300 305

gga att aca ctg cgt acc acc aag gta gga gac cgc tac gtg ctg gaa 1075

Gly Ile Thr Leu Arg Thr Thr Lys Val Gly Asp Arg Tyr Val Leu Glu

310 315 320 325

gac ctc aat gca ggt gga ttc agc ctg ggc ggc gag caa tct ggc cac 1123

Asp Leu Asn Ala Gly Gly Phe Ser Leu Gly Gly Glu Gln Ser Gly His

330 335 340

att gtt ctt cca gat cat ggc acc act ggc gat gga act ttg act ggt 1171

Ile Val Leu Pro Asp His Gly Thr Thr Gly Asp Gly Thr Leu Thr Gly

345 350 355

ctt tcc atc atg gcg cgc atg gct gaa acc gga aag tcc ttg ggc gag 1219

Leu Ser Ile Met Ala Arg Met Ala Glu Thr Gly Lys Ser Leu Gly Glu

360 365 370

ttg gca caa gct atg acg gtg ctg cca cag gtt ctg atc aat gtg cca 1267

Leu Ala Gln Ala Met Thr Val Leu Pro Gln Val Leu Ile Asn Val Pro

375 380 385

gtt tcg gat aag tcc acc atc gtg agc cac cca agc gtt gtg gct gcg 1315

Val Ser Asp Lys Ser Thr Ile Val Ser His Pro Ser Val Val Ala Ala

390 395 400 405

atc gcg gaa gca gaa gct gag ttg ggc gcc acc ggt cgc gtt ctt ctt 1363

Ile Ala Glu Ala Glu Ala Glu Leu Gly Ala Thr Gly Arg Val Leu Leu

410 415 420

cgt gct tct ggc acc gaa gag ctt ttc cgc gtg atg gtt gag gct gga 1411

Arg Ala Ser Gly Thr Glu Glu Leu Phe Arg Val Met Val Glu Ala Gly

425 430 435

gac aag gaa caa gct cgt cgt atc gcg gga cgt ctt gct gca gtg gtt 1459

Asp Lys Glu Gln Ala Arg Arg Ile Ala Gly Arg Leu Ala Ala Val Val

440 445 450

gca gaa gtc taattcactt cagtcacagc gcaacgcgat 1498

Ala Glu Val

455

<210>18

<211>456

<212>PRT

<213>谷氨酸棒状杆菌

<400>18

Leu Ala Arg Pro Cys Ile Ile Asn Arg Met Thr Arg Leu Phe Gly Thr

1 5 10 15

Asp Gly Val Arg Gly Leu Ala Asn Glu Val Leu Thr Ala Pro Leu Ala

20 25 30

Leu Lys Leu Gly Ala Ala Ala Ala His Val Leu Thr Ala Glu Lys Arg

35 40 45

Val Asp Gly Arg Arg Pro Val Ala Ile Val Gly Arg Asp Pro Arg Val

50 55 60

Ser Gly Glu Met Leu Ala Ala Ala Leu Ser Ala Gly Met Ala Ser Gln

65 70 75 80

Gly Val Asp Val Ile Arg Val Gly Val Ile Pro Thr Pro Ala Val Ala

85 90 95

Phe Leu Thr Asp Asp Tyr Gly Ala Asp Met Gly Val Met Ile Ser Ala

100 105 110

Ser His Asn Pro Met Pro Asp Asn Gly Ile Lys Phe Phe Ser Ala Gly

115 120 125

Gly His Lys Leu Pro Asp His Val Glu Asp Glu Ile Glu Arg Val Met

130 135 140

Asp Ser Leu Pro Ala Glu Gly Pro Thr Gly His Gly Val Gly Arg Val

145 150 155 160

Ile Glu Glu Ala Thr Asp Ala Gln Asp Arg Tyr Leu Glu His Leu Lys

165 170 175

Glu Ala Val Pro Thr Ser Leu Glu Gly Ile Lys Ile Val Val Asp Ala

180 185 190

Ala Asn Gly Ala Ala Ser Val Val Ala Pro Thr Ala Tyr Glu Ala Ala

195 200 205

Gly Ala Thr Val Ile Ala Ile His Asn Lys Pro Asp Ser Tyr Asn Ile

210 215 220

Asn Met Asp Cys Gly Ser Thr His Ile Asp Gln Val Gln Ala Ala Val

225 230 235 240

Leu Lys His Gly Ala Asp Leu Gly Leu Ala His Asp Gly Asp Ala Asp

245 250 255

Arg Cys Leu Ala Val Asp Lys Asp Gly Asn Leu Val Asp Gly Asp Gln

260 265 270

Ile Met Ala Leu Leu Ala Ile Ala Met Lys Glu Asn Gly Glu Leu Arg

275 280 285

Lys Asn Thr Leu Val Gly Thr Val Met Ser Asn Leu Gly Leu Lys Ile

290 295 300

Ala Met Asp Glu Ala Gly Ile Thr Leu Arg Thr Thr Lys Val Gly Asp

305 310 315 320

Arg Tyr Val Leu Glu Asp Leu Asn Ala Gly Gly Phe Ser Leu Gly Gly

325 330 335

Glu Gln Ser Gly His Ile Val Leu Pro Asp His Gly Thr Thr Gly Asp

340 345 350

Gly Thr Leu Thr Gly Leu Ser Ile Met Ala Arg Met Ala Glu Thr Gly

355 360 365

Lys Ser Leu Gly Glu Leu Ala Gln Ala Met Thr Val Leu Pro Gln Val

370 375 380

Leu Ile Asn Val Pro Val Ser Asp Lys Ser Thr Ile Val Ser His Pro

385 390 395 400

Ser Val Val Ala Ala Ile Ala Glu Ala Glu Ala Glu Leu Gly Ala Thr

405 410 415

Gly Arg Val Leu Leu Arg Ala Ser Gly Thr Glu Glu Leu Phe Arg Val

420 425 430

Met Val Glu Ala Gly Asp Lys Glu Gln Ala Arg Arg Ile Ala Gly Arg

435 440 445

Leu Ala Ala Val Val Ala Glu Val

450 455

<210>19

<211>820

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(790)

<223>RXA00526

<400>19

ggtggagcag gcggcggctc cttttagtcc tgcggcccct tttgaccctg cagcccctgc 60

cgtttctgcc aagcaaaccg tgggccaggt gatttagcct atg agc ctc atc gaa 115

Met Ser Leu Ile Glu

1 5

atg cga aat att gtc aag acc tac aac att gga tct gaa ggt gaa ctc 163

Met Arg Asn Ile Val Lys Thr Tyr Asn Ile Gly Ser Glu Gly Glu Leu

10 15 20

acc gtg ttg cac ggt gtg gat ttc cat gtg gac cgt ggc gaa ttc gtg 211

Thr Val Leu His Gly Val Asp Phe His Val Asp Arg Gly Glu Phe Val

25 30 35

tcg gtt gtg ggt acg tcc ggc tca ggt aaa tca acg atg atg aac atc 259

Ser Val Val Gly Thr Ser Gly Ser Gly Lys Ser Thr Met Met Asn Ile

40 45 50

att ggg ttg ttg gat aag cca act gat ggc acg tac acc ttg gat ggc 307

Ile Gly Leu Leu Asp Lys Pro Thr Asp Gly Thr Tyr Thr Leu Asp Gly

55 60 65

gtg gat gtg ttg gat atc agc gat gat gct ttg gcg agc cac cgc gct 355

Val Asp Val Leu Asp Ile Ser Asp Asp Ala Leu Ala Ser His Arg Ala

70 75 80 85

aaa tcg att ggt ttt gtg ttt cag aac ttc aat ctg att ggc cgg atc 403

Lys Ser Ile Gly Phe Val Phe Gln Asn Phe Asn Leu Ile Gly Arg Ile

90 95 100

gat gcg ttg aag aat gtg gaa atg ccc atg atg tat gcg ggc att ccg 451

Asp Ala Leu Lys Asn Val Glu Met Pro Met Met Tyr Ala Gly Ile Pro

105 110 115

gct aag cag cgg aga agt cgt gcg gtt gaa tta ttg gaa atg gtc ggg 499

Ala Lys Gln Arg Arg Ser Arg Ala Val Glu Leu Leu Glu Met Val Gly

120 125 130

atg ggt gag cgt ctc aac cat gag ccc aat gag ctt tcg ggt ggt cag 547

Met Gly Glu Arg Leu Asn His Glu Pro Asn Glu Leu Ser Gly Gly Gln

135 140 145

aag cag cgc gtg gcc att gct cgc gcg ttg gcg aac gat cct gag atc 595

Lys Gln Arg Val Ala Ile Ala Arg Ala Leu Ala Asn Asp Pro Glu Ile

150 155 160 165

att ctt gct gat gaa cca act ggt gcg ttg gat tct gca acg ggc cgg 643

Ile Leu Ala Asp Glu Pro Thr Gly Ala Leu Asp Ser Ala Thr Gly Arg

170 175 180

atg gtg atg gat att ttc cac cag ctc aac aag gag cag ggc aaa acc 691

Met Val Met Asp Ile Phe His Gln Leu Asn Lys Glu Gln Gly Lys Thr

185 190 195

atc gtg ttt att act cac aac cct gag ctt gct gat gaa tct gat cgg 739

Ile Val Phe Ile Thr His Asn Pro Glu Leu Ala Asp Glu Ser Asp Arg

200 205 210

gtg gtc acc atg gtt gac ggg cgc atc att ggg tct gag gtg aaa cac 787

Val Val Thr Met Val Asp Gly Arg Ile Ile Gly Ser Glu Val Lys His

215 220 225

tca tgagccttgc agaatcaatt cttttggcgc 820

Ser

230

<210>20

<211>230

<212>PRT

<213>谷氨酸棒状杆菌

<400>20

Met Ser Leu Ile Glu Met Arg Asn Ile Val Lys Thr Tyr Asn Ile Gly

1 5 10 15

Ser Glu Gly Glu Leu Thr Val Leu His Gly Val Asp Phe His Val Asp

20 25 30

Arg Gly Glu Phe Val Ser Val Val Gly Thr Ser Gly Ser Gly Lys Ser

35 40 45

Thr Met Met Asn Ile Ile Gly Leu Leu Asp Lys Pro Thr Asp Gly Thr

50 55 60

Tyr Thr Leu Asp Gly Val Asp Val Leu Asp Ile Ser Asp Asp Ala Leu

65 70 75 80

Ala Ser His Arg Ala Lys Ser Ile Gly Phe Val Phe Gln Asn Phe Asn

85 90 95

Leu Ile Gly Arg Ile Asp Ala Leu Lys Asn Val Glu Met Pro Met Met

100 105 110

Tyr Ala Gly Ile Pro Ala Lys Gln Arg Arg Ser Arg Ala Val Glu Leu

115 120 125

Leu Glu Met Val Gly Met Gly Glu Arg Leu Asn His Glu Pro Asn Glu

130 135 140

Leu Ser Gly Gly Gln Lys Gln Arg Val Ala Ile Ala Arg Ala Leu Ala

145 150 155 160

Asn Asp Pro Glu Ile Ile Leu Ala Asp Glu Pro Thr Gly Ala Leu Asp

165 170 175

Ser Ala Thr Gly Arg Met Val Met Asp Ile Phe His Gln Leu Asn Lys

180 185 190

Glu Gln Gly Lys Thr Ile Val Phe Ile Thr His Asn Pro Glu Leu Ala

195 200 205

Asp Glu Ser Asp Arg Val Val Thr Met Val Asp Gly Arg Ile Ile Gly

210 215 220

Ser Glu Val Lys His Ser

225 230

<210>21

<211>1222

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1192)

<223>RXA00683

<400>21

ggacaaagct atcgggttcc gggagaacct ctccttccgc gtccccactt ctgttcccgt 60

gacttggaac gcttaacgct ttattaaata aggagacacc atg acc aac agt ttg 115

Met Thr Asn Ser Leu

1 5

aac atc ccg ttt gtc cag cgc ttc gat gaa ggc ctg gat cct gtt cta 163

Asn Ile Pro Phe Val Gln Arg Phe Asp Glu Gly Leu Asp Pro Val Leu

10 15 20

gaa gta ctc ggt ggc aag ggc gct tca cta gtc acc atg aca gat gct 211

Glu Val Leu Gly Gly Lys Gly Ala Ser Leu Val Thr Met Thr Asp Ala

25 30 35

gga atg ccc gtt cca cct gga ttt gtg gtc act act gcc agc ttt gat 259

Gly Met Pro Val Pro Pro Gly Phe Val Val Thr Thr Ala Ser Phe Asp

40 45 50

gaa ttc atc cgt gaa gca ggg gtt gct gaa cac atc gat aaa ttc cta 307

Glu Phe Ile Arg Glu Ala Gly Val Ala Glu His Ile Asp Lys Phe Leu

55 60 65

aac gat ctc gat gca gaa gat gtt aag gaa gtg gat cga gtt tct gcg 355

Asn Asp Leu Asp Ala Glu Asp Val Lys Glu Val Asp Arg Val Ser Ala

70 75 80 85

atc atc cgc gat gag ctg tgc agt ctt gac gtt cca gag aat gct cgt 403

Ile Ile Arg Asp Glu Leu Cys Ser Leu Asp Val Pro Glu Asn Ala Arg

90 95 100

ttc gca gtg cac cag gct tat cgc gat ctc atg gaa cga tgc ggt ggc 451

Phe Ala Val His Gln Ala Tyr Arg Asp Leu Met Glu Arg Cys Gly Gly

105 110 115

gac gtc ccg gtt gct gtc cgg tca tcg gcc act gcc gaa gat ctg ccc 499

Asp Val Pro Val Ala Val Arg Ser Ser Ala Thr Ala Glu Asp Leu Pro

120 125 130

gat gct tcc ttc gca ggg caa cag gac acc tat ctg tgg caa gtc ggt 547

Asp Ala Ser Phe Ala Gly Gln Gln Asp Thr Tyr Leu Trp Gln Val Gly

135 140 145

ttg agc gct gtc act gaa cac atc cgt aaa tgc tgg gct tcg ctg ttc 595

Leu Ser Ala Val Thr Glu His Ile Arg Lys Cys Trp Ala Ser Leu Phe

150 155 160 165

act tcc cgt gcc att atc tac cgt ctg aaa aac aac atc ccc aat gag 643

Thr Ser Arg Ala Ile Ile Tyr Arg Leu Lys Asn Asn Ile Pro Asn Glu

170 175 180

ggc ctc tcc atg gcg gta gtt gtt caa aaa atg gtc aac tct cgt gtc 691

Gly Leu Ser Met Ala Val Val Val Gln Lys Met Val Asn Ser Arg Val

185 190 195

gca ggc gtg gca atc act atg aat cct tcc aac ggc gac cgc tcg aag 739

Ala Gly Val Ala Ile Thr Met Asn Pro Ser Asn Gly Asp Arg Ser Lys

200 205 210

atc acc atc gat tcc tca tgg ggt gtt ggt gaa atg gtg gtc tca ggt 787

Ile Thr Ile Asp Ser Ser Trp Gly Val Gly Glu Met Val Val Ser Gly

215 220 225

gaa gtg aca cca gac aat atc ttg ctg gac aag atc acg ctg cag gtt 835

Glu Val Thr Pro Asp Asn Ile Leu Leu Asp Lys Ile Thr Leu Gln Val

230 235 240 245

gtc tcc gaa cac att gga agc aaa cac gct gaa ctc atc ccc gat gcc 883

Val Ser Glu His Ile Gly Ser Lys His Ala Glu Leu Ile Pro Asp Ala

250 255 260

acc agt gga agc ctc gtg gaa aag ccc gtt gat gaa gaa cgc gca aac 931

Thr Ser Gly Ser Leu Val Glu Lys Pro Val Asp Glu Glu Arg Ala Asn

265 270 275

cgc cgc agt ctg act gat gag gaa atg ctc gct gtg gca caa atg gct 979

Arg Arg Ser Leu Thr Asp Glu Glu Met Leu Ala Val Ala Gln Met Ala

280 285 290

aag cgt gca gaa aaa cac tac aag tgc cca caa gat atc gaa tgg gcg 1027

Lys Arg Ala Glu Lys His Tyr Lys Cys Pro Gln Asp Ile Glu Trp Ala

295 300 305

ctg gac gct gat ctg cca gat gga gaa aac ctt ctg tta ttg caa tcc 1075

Leu Asp Ala Asp Leu Pro Asp Gly Glu Asn Leu Leu Leu Leu Gln Ser

310 315 320 325

cgc ccg gaa act atc cac tcc aac ggt gtg aag aag gaa acc cca act 1123

Arg Pro Glu Thr Ile His Ser Asn Gly Val Lys Lys Glu Thr Pro Thr

330 335 340

ccg cag gct gcc aaa acc ata ggc acc ttc gat ttc agc tca atc acc 1171

Pro Gln Ala Ala Lys Thr Ile Gly Thr Phe Asp Phe Ser Ser Ile Thr

345 350 355

gtc gca atg acc ggc acg aag taaaaccacc gcatcttttc gtcgaaaagc 1222

Val Ala Met Thr Gly Thr Lys

360

<210>22

<211>364

<212>PRT

<213>谷氨酸棒状杆菌

<400>22

Met Thr Asn Ser Leu Asn Ile Pro Phe Val Gln Arg Phe Asp Glu Gly

1 5 10 15

Leu Asp Pro Val Leu Glu Val Leu Gly Gly Lys Gly Ala Ser Leu Val

20 25 30

Thr Met Thr Asp Ala Gly Met Pro Val Pro Pro Gly Phe Val Val Thr

35 40 45

Thr Ala Ser Phe Asp Glu Phe Ile Arg Glu Ala Gly Val Ala Glu His

50 55 60

Ile Asp Lys Phe Leu Asn Asp Leu Asp Ala Glu Asp Val Lys Glu Val

65 70 75 80

Asp Arg Val Ser Ala Ile Ile Arg Asp Glu Leu Cys Ser Leu Asp Val

85 90 95

Pro Glu Asn Ala Arg Phe Ala Val His Gln Ala Tyr Arg Asp Leu Met

100 105 110

Glu Arg Cys Gly Gly Asp Val Pro Val Ala Val Arg Ser Ser Ala Thr

115 120 125

Ala Glu Asp Leu Pro Asp Ala Ser Phe Ala Gly Gln Gln Asp Thr Tyr

130 135 140

Leu Trp Gln Val Gly Leu Ser Ala Val Thr Glu His Ile Arg Lys Cys

145 150 155 160

Trp Ala Ser Leu Phe Thr Ser Arg Ala Ile Ile Tyr Arg Leu Lys Asn

165 170 175

Asn Ile Pro Asn Glu Gly Leu Ser Met Ala Val Val Val Gln Lys Met

180 185 190

Val Asn Ser Arg Val Ala Gly Val Ala Ile Thr Met Asn Pro Ser Asn

195 200 205

Gly Asp Arg Ser Lys Ile Thr Ile Asp Ser Ser Trp Gly Val Gly Glu

210 215 220

Met Val Val Ser Gly Glu Val Thr Pro Asp Asn Ile Leu Leu Asp Lys

225 230 235 240

Ile Thr Leu Gln Val Val Ser Glu His Ile Gly Ser Lys His Ala Glu

245 250 255

Leu Ile Pro Asp Ala Thr Ser Gly Ser Leu Val Glu Lys Pro Val Asp

260 265 270

Glu Glu Arg Ala Asn Arg Arg Ser Leu Thr Asp Glu Glu Met Leu Ala

275 280 285

Val Ala Gln Met Ala Lys Arg Ala Glu Lys His Tyr Lys Cys Pro Gln

290 295 300

Asp Ile Glu Trp Ala Leu Asp Ala Asp Leu Pro Asp Gly Glu Asn Leu

305 310 315 320

Leu Leu Leu Gln Ser Arg Pro Glu Thr Ile His Ser Asn Gly Val Lys

325 330 335

Lys Glu Thr Pro Thr Pro Gln Ala Ala Lys Thr Ile Gly Thr Phe Asp

340 345 350

Phe Ser Ser Ile Thr Val Ala Met Thr Gly Thr Lys

355 360

<210>23

<211>1414

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1384)

<223>RXA00684

<400>23

agtcacacct aaaagtgata gccatcacga atctttagga aaagtgattc aaacttcact 60

gtgatcggct tcggccacac acaagtgtca ggagatgaca atg act tcc cag act 115

Met Thr Ser Gln Thr

1 5

tcc caa caa tcc acc tca acc ggt gga tgc cca ttc ggg cac aca tca 163

Ser Gln Gln Ser Thr Ser Thr Gly Gly Cys Pro Phe Gly His Thr Ser

10 15 20

gag tcc acc agc cat cac ggc tac cag cct ttc gat atg cac aac ccg 211

Glu Ser Thr Ser His His Gly Tyr Gln Pro Phe Asp Met His Asn Pro

25 30 35

ttt cct gca tat aaa gaa ctc cgt cag gaa gag cca gtg atg ttc gat 259

Phe Pro Ala Tyr Lys Glu Leu Arg Gln Glu Glu Pro Val Met Phe Asp

40 45 50

gag cgc atc ggc tac tgg gtg gta acc aaa tat gac gac atc aaa acc 307

Glu Arg Ile Gly Tyr Trp Val Val Thr Lys Tyr Asp Asp Ile Lys Thr

55 60 65

acc ttt gat gac tgg gaa aca ttc tcc tct gaa aat gca caa gcc cca 355

Thr Phe Asp Asp Trp Glu Thr Phe Ser Ser Glu Asn Ala Gln Ala Pro

70 75 80 85

gtc cgc aag cgt gga cct cag gca acc caa atc atg acc gat ggc ggc 403

Val Arg Lys Arg Gly Pro Gln Ala Thr Gln Ile Met Thr Asp Gly Gly

90 95 100

ttc act gca tac tcc gga tta tca gct cgt att cca cca gag cac acc 451

Phe Thr Ala Tyr Ser Gly Leu Ser Ala Arg Ile Pro Pro Glu His Thr

105 110 115

cgc atc cgc gca atc gca caa aag gcc ttc acg cca cgc cgc tat aaa 499

Arg Ile Arg Ala Ile Ala Gln Lys Ala Phe Thr Pro Arg Arg Tyr Lys

120 125 130

gca ctc gaa cca gat atc cga gca atg gtg att gat cgt gtg gag aaa 547

Ala Leu Glu Pro Asp Ile Arg Ala Met Val Ile Asp Arg Val Glu Lys

135 140 145

atg ttg gcg aat gat caa cac gtc ggc gat atg gtg tca gat ctt gcc 595

Met Leu Ala Asn Asp Gln His Val Gly Asp Met Val Ser Asp Leu Ala

150 155 160 165

tac gac att cca acc atc acg atc ctg acg ctg atc ggt gca gat att 643

Tyr Asp Ile Pro Thr Ile Thr Ile Leu Thr Leu Ile Gly Ala Asp Ile

170 175 180

tcc atg gtg gac acc tac aag cgg tgg tca gat tcc cgt gcg gcc atg 691

Ser Met Val Asp Thr Tyr Lys Arg Trp Ser Asp Ser Arg Ala Ala Met

185 190 195

acc tgg ggc gat ctt agt gat gaa gag cag atc cca cac gca cac aat 739

Thr Trp Gly Asp Leu Ser Asp Glu Glu Gln Ile Pro His Ala His Asn

200 205 210

ttg gtt gag tac tgg cag gaa tgc caa cgc atg gta gct gat gca cat 787

Leu Val Glu Tyr Trp Gln Glu Cys Gln Arg Met Val Ala Asp Ala His

215 220 225

gca cac ggt ggc gac aac ctc acc gct gat cta gtg cga gca cag caa 835

Ala His Gly Gly Asp Asn Leu Thr Ala Asp Leu Val Arg Ala Gln Gln

230 235 240 245

gag ggt caa gaa atc acc gat cat gag att gct tct ttg ctg tac tcc 883

Glu Gly Gln Glu Ile Thr Asp His Glu Ile Ala Ser Leu Leu Tyr Ser

250 255 260

ctg ctt ttt gcg ggg cac gaa aca acc acc acg ttg atc tcc aat tgt 931

Leu Leu Phe Ala Gly His Glu Thr Thr Thr Thr Leu Ile Ser Asn Cys

265 270 275

ttc cga gtt ctc ctc gat cat cca gag cag tgg caa gcc att cta gag 979

Phe Arg Val Leu Leu Asp His Pro Glu Gln Trp Gln Ala Ile Leu Glu

280 285 290

aat cca aaa ctg att cct gcg gca gtg gat gag gtc ttg cgg tac tcc 1027

Asn Pro Lys Leu Ile Pro Ala Ala Val Asp Glu Val Leu Arg Tyr Ser

295 300 305

ggc tcg atc gtg ggg tgg cgt cga aaa gca tta aaa gac acc gag atc 1075

Gly Ser Ile Val Gly Trp Arg Arg Lys Ala Leu Lys Asp Thr Glu Ile

310 315 320 325

ggc ggc gtt gcc att aag gaa ggc gat ggt gtt ctg ctg ctc atg ggt 1123

Gly Gly Val Ala Ile Lys Glu Gly Asp Gly Val Leu Leu Leu Met Gly

330 335 340

tcc gcg aac cgc gat gaa gct cgc ttt gaa aat ggc gag gaa ttc gat 1171

Ser Ala Asn Arg Asp Glu Ala Arg Phe Glu Asn Gly Glu Glu Phe Asp

345 350 355

atc agc cgc gct aat gcg cgc gag cac ctg tct ttt ggt ttc ggc atc 1219

Ile Ser Arg Ala Asn Ala Arg Glu His Leu Ser Phe Gly Phe Gly Ile

360 365 370

cac tat tgc cta gga aac atg ctg gcc aaa ctt caa gcc aag atc tgt 1267

His Tyr Cys Leu Gly Asn Met Leu Ala Lys Leu Gln Ala Lys Ile Cys

375 380 385

ctc gag gaa gtc acc agg ctt gtt cct tcc ctg cac ttg gtt gcg gac 1315

Leu Glu Glu Val Thr Arg Leu Val Pro Ser Leu His Leu Val Ala Asp

390 395 400 405

aaa gct atc ggg ttc cgg gag aac ctc tcc ttc cgc gtc ccc act tct 1363

Lys Ala Ile Gly Phe Arg Glu Asn Leu Ser Phe Arg Val Pro Thr Ser

410 415 420

gtt ccc gtg act tgg aac gct taacgcttta ttaaataagg agacaccatg 1414

Val Pro Val Thr Trp Asn Ala

425

<210>24

<211>428

<212>PRT

<213>谷氨酸棒状杆菌

<400>24

Met Thr Ser Gln Thr Ser Gln Gln Ser Thr Ser Thr Gly Gly Cys Pro

1 5 10 15

Phe Gly His Thr Ser Glu Ser Thr Ser His His Gly Tyr Gln Pro Phe

20 25 30

Asp Met His Asn Pro Phe Pro Ala Tyr Lys Glu Leu Arg Gln Glu Glu

35 40 45

Pro Val Met Phe Asp Glu Arg Ile Gly Tyr Trp Val Val Thr Lys Tyr

50 55 60

Asp Asp Ile Lys Thr Thr Phe Asp Asp Trp Glu Thr Phe Ser Ser Glu

65 70 75 80

Asn Ala Gln Ala Pro Val Arg Lys Arg Gly Pro Gln Ala Thr Gln Ile

85 90 95

Met Thr Asp Gly Gly Phe Thr Ala Tyr Ser Gly Leu Ser Ala Arg Ile

100 105 110

Pro Pro Glu His Thr Arg Ile Arg Ala Ile Ala Gln Lys Ala Phe Thr

115 120 125

Pro Arg Arg Tyr Lys Ala Leu Glu Pro Asp Ile Arg Ala Met Val Ile

130 135 140

Asp Arg Val Glu Lys Met Leu Ala Asn Asp Gln His Val Gly Asp Met

145 150 155 160

Val Ser Asp Leu Ala Tyr Asp Ile Pro Thr Ile Thr Ile Leu Thr Leu

165 170 175

Ile Gly Ala Asp Ile Ser Met Val Asp Thr Tyr Lys Arg Trp Ser Asp

180 185 190

Ser Arg Ala Ala Met Thr Trp Gly Asp Leu Ser Asp Glu Glu Gln Ile

195 200 205

Pro His Ala His Asn Leu Val Glu Tyr Trp Gln Glu Cys Gln Arg Met

210 215 220

Val Ala Asp Ala His Ala His Gly Gly Asp Asn Leu Thr Ala Asp Leu

225 230 235 240

Val Arg Ala Gln Gln Glu Gly Gln Glu Ile Thr Asp His Glu Ile Ala

245 250 255

Ser Leu Leu Tyr Ser Leu Leu Phe Ala Gly His Glu Thr Thr Thr Thr

260 265 270

Leu Ile Ser Asn Cys Phe Arg Val Leu Leu Asp His Pro Glu Gln Trp

275 280 285

Gln Ala Ile Leu Glu Asn Pro Lys Leu Ile Pro Ala Ala Val Asp Glu

290 295 300

Val Leu Arg Tyr Ser Gly Ser Ile Val Gly Trp Arg Arg Lys Ala Leu

305 310 315 320

Lys Asp Thr Glu Ile Gly Gly Val Ala Ile Lys Glu Gly Asp Gly Val

325 330 335

Leu Leu Leu Met Gly Ser Ala Asn Arg Asp Glu Ala Arg Phe Glu Asn

340 345 350

Gly Glu Glu Phe Asp Ile Ser Arg Ala Asn Ala Arg Glu His Leu Ser

355 360 365

Phe Gly Phe Gly Ile His Tyr Cys Leu Gly Asn Met Leu Ala Lys Leu

370 375 380

Gln Ala Lys Ile Cys Leu Glu Glu Val Thr Arg Leu Val Pro Ser Leu

385 390 395 400

His Leu Val Ala Asp Lys Ala Ile Gly Phe Arg Glu Asn Leu Ser Phe

405 410 415

Arg Val Pro Thr Ser Val Pro Val Thr Trp Asn Ala

420 425

<210>25

<211>1402

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1372)

<223>RXA00783

<400>25

aaagttccca aggggtgggg gctgagcacc acggatccaa ttttgttgca atttgcaaag 60

tttacagtgt tagacttcac aatacgatca tattggtgag ttg aaa cac tta ctt 115

Leu Lys His Leu Leu

1 5

tta cgg gaa gac ttt gtt aaa gac gca gaa ggc tct aag cat ggg ccg 163

Leu Arg Glu Asp Phe Val Lys Asp Ala Glu Gly Ser Lys His Gly Pro

10 15 20

gaa atg gaa ttg gca gtg gat ctt ttt gaa tac caa gca cgg gac ctc 211

Glu Met Glu Leu Ala Val Asp Leu Phe Glu Tyr Gln Ala Arg Asp Leu

25 30 35

ttt gaa acc cat ggt gtg cca gtg ttg aag gga att gtg gca tca aca 259

Phe Glu Thr His Gly Val Pro Val Leu Lys Gly Ile Val Ala Ser Thr

40 45 50

cca gag gcg gcg agg aaa gcg gct gag gaa atc ggc gga ctg acc gtc 307

Pro Glu Ala Ala Arg Lys Ala Ala Glu Glu Ile Gly Gly Leu Thr Val

55 60 65

gtc aag gct cag gtc aag gtg ggc gga cgt ggc aag gcg ggt ggc gtc 355

Val Lys Ala Gln Val Lys Val Gly Gly Arg Gly Lys Ala Gly Gly Val

70 75 80 85

cgt gtg gca ccg acg tcg gct cag gct ttt gat gct gcg gat gcg att 403

Arg Val Ala Pro Thr Ser Ala Gln Ala Phe Asp Ala Ala Asp Ala Ile

90 95 100

ctc ggc atg gat atc aaa gga cac act gtt aat cag gtg atg gtg gcg 451

Leu Gly Met Asp Ile Lys Gly His Thr Val Asn Gln Val Met Val Ala

105 110 115

cag ggc gct gac att gct gag gaa tac tat ttc tcc att ttg ttg gat 499

Gln Gly Ala Asp Ile Ala Glu Glu Tyr Tyr Phe Ser Ile Leu Leu Asp

120 125 130

cgc gcg aat cgt tcg tat ctg gct atg tgc tct gtt gaa ggt ggc atg 547

Arg Ala Asn Arg Ser Tyr Leu Ala Met Cys Ser Val Glu Gly Gly Met

135 140 145

gag atc gag atc ctg gcg aag gaa aag cct gaa gct ttg gca aag gtg 595

Glu Ile Glu Ile Leu Ala Lys Glu Lys Pro Glu Ala Leu Ala Lys Val

150 155 160 165

gaa gtg gat ccc ctc act ggt att gat gag gac aaa gcg cgg gag att 643

Glu Val Asp Pro Leu Thr Gly Ile Asp Glu Asp Lys Ala Arg Glu Ile

170 175 180

gtc act gct gct ggc ttt gaa act gag gtg gca gag aaa gtc att ccg 691

Val Thr Ala Ala Gly Phe Glu Thr Glu Val Ala Glu Lys Val Ile Pro

185 190 195

gtg ctg atc aag atc tgg cag gtg tat tac gaa gag gaa gca aca ctc 739

Val Leu Ile Lys Ile Trp Gln Val Tyr Tyr Glu Glu Glu Ala Thr Leu

200 205 210

gtt gag gtg aac ccg ttg gtg ctc acg gat gac ggc gat gtg att gcg 787

Val Glu Val Asn Pro Leu Val Leu Thr Asp Asp Gly Asp Val Ile Ala

215 220 225

ctt gat ggc aag atc acg ctg gat gat aac gct gat ttc cgc cat gat 835

Leu Asp Gly Lys Ile Thr Leu Asp Asp Asn Ala Asp Phe Arg His Asp

230 235 240 245

aac cgt ggt gcg ttg gct gaa tct gcc ggt ggc ttg gac att ttg gaa 883

Asn Arg Gly Ala Leu Ala Glu Ser Ala Gly Gly Leu Asp Ile Leu Glu

250 255 260

ctg aag gcc aag aag aat gat ctg aac tac gtg aaa ctt gat ggc tct 931

Leu Lys Ala Lys Lys Asn Asp Leu Asn Tyr Val Lys Leu Asp Gly Ser

265 270 275

gtg ggc atc att ggc aat ggt gca ggt ttg gtg atg tcc acg ttg gat 979

Val Gly Ile Ile Gly Asn Gly Ala Gly Leu Val Met Ser Thr Leu Asp

280 285 290

atc gtg gct gca gct ggt gaa cgc cat ggt ggg cag cgc ccc gcg aac 1027

Ile Val Ala Ala Ala Gly Glu Arg His Gly Gly Gln Arg Pro Ala Asn

295 300 305

ttc cta gac att ggt ggc gga gca tca gct gaa tcg atg gct gct ggt 1075

Phe Leu Asp Ile Gly Gly Gly Ala Ser Ala Glu Ser Met Ala Ala Gly

310 315 320 325

ctc gat gtg atc ctt ggg gat agc cag gta cgc agt gtg ttt gtg aat 1123

Leu Asp Val Ile Leu Gly Asp Ser Gln Val Arg Ser Val Phe Val Asn

330 335 340

gtg ttt ggt ggc atc acc gcg tgt gat gtg gtg gca aag gga atc gtt 1171

Val Phe Gly Gly Ile Thr Ala Cys Asp Val Val Ala Lys Gly Ile Val

345 350 355

gga gct ttg gat gtg ctc ggc gat caa gca acg aag cct ctt gtg gtg 1219

Gly Ala Leu Asp Val Leu Gly Asp Gln Ala Thr Lys Pro Leu Val Val

360 365 370

cgc ctt gat ggc aac aac gtg gtg gaaggc aga cga atc ctc gcg gaa 1267

Arg Leu Asp Gly Asn Asn Val Val Glu Gly Arg Arg Ile Leu Ala Glu

375 380 385

tat aac cac cct ttg gtc acc gtt gtg gag ggt atg gat gca gcg gct 1315

Tyr Asn His Pro Leu Val Thr Val Val Glu Gly Met Asp Ala Ala Ala

390 395 400 405

gat cac gct gcc cat ttg gcc aat ctt gcc cag cac ggc cag ttc gca 1363

Asp His Ala Ala His Leu Ala Asn Leu Ala Gln His Gly Gln Phe Ala

410 415 420

acc gct aat tagttaagga gcacctgttt aatcatgtct 1402

Thr Ala Asn

<210>26

<211>424

<212>PRT

<213>谷氨酸棒状杆菌

<400>26

Leu Lys His Leu Leu Leu Arg Glu Asp Phe Val Lys Asp Ala Glu Gly

1 5 10 15

Ser Lys His Gly Pro Glu Met Glu Leu Ala Val Asp Leu Phe Glu Tyr

20 25 30

Gln Ala Arg Asp Leu Phe Glu Thr His Gly Val Pro Val Leu Lys Gly

35 40 45

Ile Val Ala Ser Thr Pro Glu Ala Ala Arg Lys Ala Ala Glu Glu Ile

50 55 60

Gly Gly Leu Thr Val Val Lys Ala Gln Val Lys Val Gly Gly Arg Gly

65 70 75 80

Lys Ala Gly Gly Val Arg Val Ala Pro Thr Ser Ala Gln Ala Phe Asp

85 90 95

Ala Ala Asp Ala Ile Leu Gly Met Asp Ile Lys Gly His Thr Val Asn

100 105 110

Gln Val Met Val Ala Gln Gly Ala Asp Ile Ala Glu Glu Tyr Tyr Phe

115 120 125

Ser Ile Leu Leu Asp Arg Ala Asn Arg Ser Tyr Leu Ala Met Cys Ser

130 135 140

Val Glu Gly Gly Met Glu Ile Glu Ile Leu Ala Lys Glu Lys Pro Glu

145 150 155 160

Ala Leu Ala Lys Val Glu Val Asp Pro Leu Thr Gly Ile Asp Glu Asp

165 170 175

Lys Ala Arg Glu Ile Val Thr Ala Ala Gly Phe Glu Thr Glu Val Ala

180 185 190

Glu Lys Val Ile Pro Val Leu Ile Lys Ile Trp Gln Val Tyr Tyr Glu

195 200 205

Glu Glu Ala Thr Leu Val Glu Val Asn Pro Leu Val Leu Thr Asp Asp

210 215 220

Gly Asp Val Ile Ala Leu Asp Gly Lys Ile Thr Leu Asp Asp Asn Ala

225 230 235 240

Asp Phe Arg His Asp Asn Arg Gly Ala Leu Ala Glu Ser Ala Gly Gly

245 250 255

Leu Asp Ile Leu Glu Leu Lys Ala Lys Lys Asn Asp Leu Asn Tyr Val

260 265 270

Lys Leu Asp Gly Ser Val Gly Ile Ile Gly Asn Gly Ala Gly Leu Val

275 280 285

Met Ser Thr Leu Asp Ile Val Ala Ala Ala Gly Glu Arg His Gly Gly

290 295 300

Gln Arg Pro Ala Asn Phe Leu Asp Ile Gly Gly Gly Ala Ser Ala Glu

305 310 315 320

Ser Met Ala Ala Gly Leu Asp Val Ile Leu Gly Asp Ser Gln Val Arg

325 330 335

Ser Val Phe Val Asn Val Phe Gly Gly Ile Thr Ala Cys Asp Val Val

340 345 350

Ala Lys Gly Ile Val Gly Ala Leu Asp Val Leu Gly Asp Gln Ala Thr

355 360 365

Lys Pro Leu Val Val Arg Leu Asp Gly Asn Asn Val Val Glu Gly Arg

370 375 380

Arg Ile Leu Ala Glu Tyr Asn His Pro Leu Val Thr Val Val Glu Gly

385 390 395 400

Met Asp Ala Ala Ala Asp His Ala Ala His Leu Ala Asn Leu Ala Gln

405 410 415

His Gly Gln Phe Ala Thr Ala Asn

420

<210>27

<211>1072

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1042)

<223>RXA00825

<400>27

gctcagttac ccgttcatgc tgggctttgg tgcggtgatg gcaactattt gtctgatcat 60

tgtgagtttt agtgcacgcc gattctgaga aacaactaaa gtg agc cac atg cgc 115

Val Ser His Met Arg

1 5

aca gta gtt acc ggc ggt gcc ggc ttc atc gga tcc cat ctc gtt gac 163

Thr Val Val Thr Gly Gly Ala Gly Phe Ile Gly Ser His Leu Val Asp

10 15 20

ctt ttg atc aag gaa ggc cac gag gtc gtt gtg atc gat aac ctc tcc 211

Leu Leu Ile Lys Glu Gly His Glu Val Val Val Ile Asp Asn Leu Ser

25 30 35

cgc gga cgc ctg gag aat ctc tcc gat gcg gaa gcc acc gga aaa ctc 259

Arg Gly Arg Leu Glu Asn Leu Ser Asp Ala Glu Ala Thr Gly Lys Leu

40 45 50

acc ttt gtg gaa gcc gat ctt ctc gac gtt gat ttc aac gag ttt cta 307

Thr Phe Val Glu Ala Asp Leu Leu Asp Val Asp Phe Asn Glu Phe Leu

55 60 65

gga acc cac aag cct gag gtt att ttc cac ctg gca gcg caa atc gat 355

Gly Thr His Lys Pro Glu Val Ile Phe His Leu Ala Ala Gln Ile Asp

70 75 80 85

gtg cgc cac tct gtt gta gat cct ctt cac gac gcc gaa acc aac att 403

Val Arg His Ser Val Val Asp Pro Leu His Asp Ala Glu Thr Asn Ile

90 95 100

ttg tcc acc atc cgc atc gct gac gct gcc cgc cag cac ggt gtt cgc 451

Leu Ser Thr Ile Arg Ile Ala Asp Ala Ala Arg Gln His Gly Val Arg

105 110 115

aag gtt gtc ttt acc tcc tca ggc ggt tcc att tac ggt gag cct tcg 499

Lys Val Val Phe Thr Ser Ser Gly Gly Ser Ile Tyr Gly Glu Pro Ser

120 125 130

gaa ttc cca gtt gat gaa acc gtg cca gtg gat cca cat tcc cct tat 547

Glu Phe Pro Val Asp Glu Thr Val Pro Val Asp Pro His Ser Pro Tyr

135 140 145

gcg gca tcc aag gtg tcc ggt gaa att tac ctg aac acc ttc cgc cac 595

Ala Ala Ser Lys Val Ser Gly Glu Ile Tyr Leu Asn Thr Phe Arg His

150 155 160 165

ctg tac ggc tta gac tgt tct cac atc gca ccg gca aat gtt tac ggc 643

Leu Tyr Gly Leu Asp Cys Ser His Ile Ala Pro Ala Asn Val Tyr Gly

170 175 180

cca cgc caa gat cca cac ggt gaa gca gga gtt gtg gcc att ttc gcg 691

Pro Arg Gln Asp Pro His Gly Glu Ala Gly Val Val Ala Ile Phe Ala

185 190 195

ctg cga ctt ctg gga ggc ctg gac acc aag gta ttc ggc gac ggc gga 739

Leu Arg Leu Leu Gly Gly Leu Asp Thr Lys Val Phe Gly Asp Gly Gly

200 205 210

aac acc cgc gac tac gtc tac gtc ggt gac gta gtt cgt gct ttc tac 787

Asn Thr Arg Asp Tyr Val Tyr Val Gly Asp Val Val Arg Ala Phe Tyr

215 220 225

ctg gct tct ggg gaa atc ggt ggg gga gag cgc ttc aac att ggc acc 835

Leu Ala Ser Gly Glu Ile Gly Gly Gly Glu Arg Phe Asn Ile Gly Thr

230 235 240 245

tct gtg gaa acc tct gac cgc cag ctg cac acc ctc gtg gcc act gcg 883

Ser Val Glu Thr Ser Asp Arg Gln Leu His Thr Leu Val Ala Thr Ala

250 255 260

gca ggt tcc aaa gat gat cct gaa tat gca cct gca cgt ctc ggc gat 931

Ala Gly Ser Lys Asp Asp Pro Glu Tyr Ala Pro Ala Arg Leu Gly Asp

265 270 275

gtg cca cgc agt gca ctc agc ttc ggc aag gcc aaa gag gtg ctt ggt 979

Val Pro Arg Ser Ala Leu Ser Phe Gly Lys Ala Lys Glu Val Leu Gly

280 285 290

tgg gag cct gag gtg aac atc gaa caa ggt gtg gcc aag act gtg gag 1027

Trp Glu Pro Glu Val Asn Ile Glu Gln Gly Val Ala Lys Thr Val Glu

295 300 305

tac ttc cgc act cac taggggaaaa tccaccacaa atcgctctga 1072

Tyr Phe Arg Thr His

310

<210>28

<211>314

<212>PRT

<213>谷氨酸棒状杆菌

<400>28

Val Ser His Met Arg Thr Val Val Thr Gly Gly Ala Gly Phe Ile Gly

1 5 10 15

Ser His Leu Val Asp Leu Leu Ile Lys Glu Gly His Glu Val Val Val

20 25 30

Ile Asp Asn Leu Ser Arg Gly Arg Leu Glu Asn Leu Ser Asp Ala Glu

35 40 45

Ala Thr Gly Lys Leu Thr Phe Val Glu Ala Asp Leu Leu Asp Val Asp

50 55 60

Phe Asn Glu Phe Leu Gly Thr His Lys Pro Glu Val Ile Phe His Leu

65 70 75 80

Ala Ala Gln Ile Asp Val Arg His Ser Val Val Asp Pro Leu His Asp

85 90 95

Ala Glu Thr Asn Ile Leu Ser Thr Ile Arg Ile Ala Asp Ala Ala Arg

100 105 110

Gln His Gly Val Arg Lys Val Val Phe Thr Ser Ser Gly Gly Ser Ile

115 120 125

Tyr Gly Glu Pro Ser Glu Phe Pro Val Asp Glu Thr Val Pro Val Asp

130 135 140

Pro His Ser Pro Tyr Ala Ala Ser Lys Val Ser Gly Glu Ile Tyr Leu

145 150 155 160

Asn Thr Phe Arg His Leu Tyr Gly Leu Asp Cys Ser His Ile Ala Pro

165 170 175

Ala Asn Val Tyr Gly Pro Arg Gln Asp Pro His Gly Glu Ala Gly Val

180 185 190

Val Ala Ile Phe Ala Leu Arg Leu Leu Gly Gly Leu Asp Thr Lys Val

195 200 205

Phe Gly Asp Gly Gly Asn Thr Arg Asp Tyr Val Tyr Val Gly Asp Val

210 215 220

Val Arg Ala Phe Tyr Leu Ala Ser Gly Glu Ile Gly Gly Gly Glu Arg

225 230 235 240

Phe Asn Ile Gly Thr Ser Val Glu Thr Ser Asp Arg Gln Leu His Thr

245 250 255

Leu Val Ala Thr Ala Ala Gly Ser Lys Asp Asp Pro Glu Tyr Ala Pro

260 265 270

Ala Arg Leu Gly Asp Val Pro Arg Ser Ala Leu Ser Phe Gly Lys Ala

275 280 285

Lys Glu Val Leu Gly Trp Glu Pro Glu Val Asn Ile Glu Gln Gly Val

290 295 300

Ala Lys Thr Val Glu Tyr Phe Arg Thr His

305 310

<210>29

<211>2041

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101).. (2011)

<223>RXA00868

<400>29

cgatgaccca gcgcacgcct gggttcgaga tacatggacc gggcaagcat ttgatgatcg 60

cttgccatat gagaacgcaa acaaggaggg ataaaatttc atg gct gaa acg aag 115

Met Ala Glu Thr Lys

1 5

aga atg aca gtt agc cag gca ctg gtt gaa ttc ctt ggt cac cag tgg 163

Arg Met Thr Val Ser Gln Ala Leu Val Glu Phe Leu Gly His Gln Trp

10 15 20

act gtc gac ggc gat atc cgc gag cgc acc att cca ggc atg ttc gga 211

Thr Val Asp Gly Asp Ile Arg Glu Arg Thr Ile Pro Gly Met Phe Gly

25 30 35

att ttc gga cac gga aac gtt gct ggc att ggc cag gca ctc aag cag 259

Ile Phe Gly His Gly Asn Val Ala Gly Ile Gly Gln Ala Leu Lys Gln

40 45 50

tac aac gtt gaa caa cct gag ctc atg ccg tac tac cag gct cgt aat 307

Tyr Asn Val Glu Gln Pro Glu Leu Met Pro Tyr Tyr Gln Ala Arg Asn

55 60 65

gag cag gcg atg gtg cac cag tct gtt gga tat gca cgc atg cac cgc 355

Glu Gln Ala Met Val His Gln Ser Val Gly Tyr Ala Arg Met His Arg

70 75 80 85

cgt cgt ggc aca tac gca tct gcc gca tct gtt gga ccc ggc gcg acc 403

Arg Arg Gly Thr Tyr Ala Ser Ala Ala Ser Val Gly Pro Gly Ala Thr

90 95 100

aac ctg tta acc ggt gcg gct ctt gct acc acc aac cgt ttg cca gcg 451

Asn Leu Leu Thr Gly Ala Ala Leu Ala Thr Thr Asn Arg Leu Pro Ala

105 110 115

ttg ctg ctg cct agt gat act ttt gcc acc cgc gtg gcg gat cca gtg 499

Leu Leu Leu Pro Ser Asp Thr Phe Ala Thr Arg Val Ala Asp Pro Val

120 125 130

ttg cag cag ttg gag cag cca tgg gat atc ggg ctg acg gtt aat gat 547

Leu Gln Gln Leu Glu Gln Pro Trp Asp Ile Gly Leu Thr Val Asn Asp

135 140 145

gct ttc cgc cct gtg tct aag ttc ttt gat cgg gtg cag cgc ccg gag 595

Ala Phe Arg Pro Val Ser Lys Phe Phe Asp Arg Val Gln Arg Pro Glu

150 155 160 165

cag ttg ttc tct att gcg ttg gct gcg atg cgt gtg ttg act gat ccc 643

Gln Leu Phe Ser Ile Ala Leu Ala Ala Met Arg Val Leu Thr Asp Pro

170 175 180

gca gaa acc ggt gcg gtc acc att gcg ctt cca gaa gat gtg cag gct 691

Ala Glu Thr Gly Ala Val Thr Ile Ala Leu Pro Glu Asp Val Gln Ala

185 190 195

gaa atg ctc gat gtg ccg gtg gag ttc ttg cag gat cgt gag tgg cac 739

Glu Met Leu Asp Val Pro Val Glu Phe Leu Gln Asp Arg Glu Trp His

200 205 210

att agg cgc cca cgt cca gag cgt gct gcg ttg gct cgt gcg att gaa 787

Ile Arg Arg Pro Arg Pro Glu Arg Ala Ala Leu Ala Arg Ala Ile Glu

215 220 225

gtc atc aaa aac gct aag aat ccg atg atc att gct ggt ggc gga gtg 835

Val Ile Lys Asn Ala Lys Asn Pro Met Ile Ile Ala Gly Gly Gly Val

230 235 240 245

ttg tac tcc gat gcg gaa acg cag ctg cag gca ctt gtg gag cag act 883

Leu Tyr Ser Asp Ala Glu Thr Gln Leu Gln Ala Leu Val Glu Gln Thr

250 255 260

ggc att cca gtg ggt acc tcc caa gct ggt ggt ggc gtg ttg gcg tgg 931

Gly Ile Pro Val Gly Thr Ser Gln Ala Gly Gly Gly Val Leu Ala Trp

265 270 275

gat cat gca caa aac tta ggt ggt gtg ggt gcc acc gga acg ttg gct 979

Asp His Ala Gln Asn Leu Gly Gly Val Gly Ala Thr Gly Thr Leu Ala

280 285 290

gcc aac cgc att gcg ggt gat gct gat gtg atc atc ggt atc ggt act 1027

Ala Asn Arg Ile Ala Gly Asp Ala Asp Val Ile Ile Gly Ile Gly Thr

295 300 305

cgt tac agc gat ttc acc acc gca tct cgc act gcg ttc caa aac cct 1075

Arg Tyr Ser Asp Phe Thr Thr Ala Ser Arg Thr Ala Phe Gln Asn Pro

310 315 320 325

gat gtc acc ttc atc aac atc aat gtt gct tcc ttc gat gct tac aag 1123

Asp Val Thr Phe Ile Asn Ile Asn Val Ala Ser Phe Asp Ala Tyr Lys

330 335 340

cat ggc act cag ttg cct gtg att gca gat gca cgc gag gca att gtg 1171

His Gly Thr Gln Leu Pro Val Ile Ala Asp Ala Arg Glu Ala Ile Val

345 350 355

gag ctt gct gaa gcc ctg cag gga ttc acc gtg gca gag gat tac gcg 1219

Glu Leu Ala Glu Ala Leu Gln Gly Phe Thr Val Ala Glu Asp Tyr Ala

360 365 370

cag cgc atc gcg aag gaa aag gct gcg tgg gac gca gaa gta gat aag 1267

Gln Arg Ile Ala Lys Glu Lys Ala Ala Trp Asp Ala Glu Val Asp Lys

375 380 385

tct ttt gcc ccc tcc ggt ctt gcg ctg cct gga cag ccg gag atc atc 1315

Ser Phe Ala Pro Ser Gly Leu Ala Leu Pro Gly Gln Pro Glu Ile Ile

390 395 400 405

ggc gcg gtg cag gcg tcg aca agc gaa aaa gac gtc att gtg cag gcc 1363

Gly Ala Val Gln Ala Ser Thr Ser Glu Lys Asp Val Ile Val Gln Ala

410 415 420

gct gga tcc ttg cct ggt gac ctg cac aag ctg tgg cgt gtg cgc gat 1411

Ala Gly Ser Leu Pro Gly Asp Leu His Lys Leu Trp Arg Val Arg Asp

425 430 435

gcg ctg ggc tac cac gtg gaa tat gcg ttc tcg tgc atg ggc tat gaa 1459

Ala Leu Gly Tyr His Val Glu Tyr Ala Phe Ser Cys Met Gly Tyr Glu

440 445 450

atc gcg ggc ggt atc ggc gcg aag cgt gga ctt gat gcc gca ggc gat 1507

Ile Ala Gly Gly Ile Gly Ala Lys Arg Gly Leu Asp Ala Ala Gly Asp

455 460 465

gac cgc gac gtg gtg atc atg gtt ggt gat ggg tcc tac ctc atg ctc 1555

Asp Arg Asp Val Val Ile Met Val Gly Asp Gly Ser Tyr Leu Met Leu

470 475 480 485

aac act gag ctg gtc acg gcc gtg gca gaa ggt atc aag gtg att gtg 1603

Asn Thr Glu Leu Val Thr Ala Val Ala Glu Gly Ile Lys Val Ile Val

490 495 500

gtg ctc atc caa aac cac ggt tat gcc tcc atc ggc cac ctg tct gaa 1651

Val Leu Ile Gln Asn His Gly Tyr Ala Ser Ile Gly His Leu Ser Glu

505 510 515

act gtc ggt tcg cag cgt ttt ggt act tgg tac cgc gaa tat gac gct 1699

Thr Val Gly Ser Gln Arg Phe Gly Thr Trp Tyr Arg Glu Tyr Asp Ala

520 525 530

gag gcg aaa aac ttc cag ggc gag cag att ctg cct gtt gac ctg gcg 1747

Glu Ala Lys Asn Phe Gln Gly Glu Gln Ile Leu Pro Val Asp Leu Ala

535 540 545

atg aat gca cgc agc tac ggc atg gat gtc att gaa gtg gaa cca agc 1795

Met Asn Ala Arg Ser Tyr Gly Met Asp Val Ile Glu Val Glu Pro Ser

550 555 560 565

gcg aat gcg atc gag gat ctc aaa gca gcg atg gca acc gcg aag gct 1843

Ala Asn Ala Ile Glu Asp Leu Lys Ala Ala Met Ala Thr Ala Lys Ala

570 575 580

tcg gag aaa tcc acc ttc atc cac atc aac agc gat ccg ttg atc tac 1891

Ser Glu Lys Ser Thr Phe Ile His Ile Asn Ser Asp Pro Leu Ile Tyr

585 590 595

gca cca gac ggt gct ggt tgg tgg gac gtg ccg gtg tcg gag acg tcc 1939

Ala Pro Asp Gly Ala Gly Trp Trp Asp Val Pro Val Ser Glu Thr Ser

600 605 610

act ctg gat agc acc aac gcg gct cgt gaa gat tac ctg aaa aac caa 1987

Thr Leu Asp Ser Thr Asn Ala Ala Arg Glu Asp Tyr Leu Lys Asn Gln

615 620 625

gcc ctc cag cgt ccg ctg ctc ggc taaaccagtt ggctaaacca aaacgtattt 2041

Ala Leu Gln Arg Pro Leu Leu Gly

630 635

<210>30

<211>637

<212>PRT

<213>谷氨酸棒状杆菌

<400>30

Met Ala Glu Thr Lys Arg Met Thr Val Ser Gln Ala Leu Val Glu Phe

1 5 10 15

Leu Gly His Gln Trp Thr Val Asp Gly Asp Ile Arg Glu Arg Thr Ile

20 25 30

Pro Gly Met Phe Gly Ile Phe Gly His Gly Asn Val Ala Gly Ile Gly

35 40 45

Gln Ala Leu Lys Gln Tyr Asn Val Glu Gln Pro Glu Leu Met Pro Tyr

50 55 60

Tyr Gln Ala Arg Asn Glu Gln Ala Met Val His Gln Ser Val Gly Tyr

65 70 75 80

Ala Arg Met His Arg Arg Arg Gly Thr Tyr Ala Ser Ala Ala Ser Val

85 90 95

Gly Pro Gly Ala Thr Asn Leu Leu Thr Gly Ala Ala Leu Ala Thr Thr

100 105 110

Asn Arg Leu Pro Ala Leu Leu Leu Pro Ser Asp Thr Phe Ala Thr Arg

115 120 125

Val Ala Asp Pro Val Leu Gln Gln Leu Glu Gln Pro Trp Asp Ile Gly

130 135 140

Leu Thr Val Asn Asp Ala Phe Arg Pro Val Ser Lys Phe Phe Asp Arg

145 150 155 160

Val Gln Arg Pro Glu Gln Leu Phe Ser Ile Ala Leu Ala Ala Met Arg

165 170 175

Val Leu Thr Asp Pro Ala Glu Thr Gly Ala Val Thr Ile Ala Leu Pro

180 185 190

Glu Asp Val Gln Ala Glu Met Leu Asp Val Pro Val Glu Phe Leu Gln

195 200 205

Asp Arg Glu Trp His Ile Arg Arg Pro Arg Pro Glu Arg Ala Ala Leu

210 215 220

Ala Arg Ala Ile Glu Val Ile Lys Asn Ala Lys Asn Pro Met Ile Ile

225 230 235 240

Ala Gly Gly Gly Val Leu Tyr Ser Asp Ala Glu Thr Gln Leu Gln Ala

245 250 255

Leu Val Glu Gln Thr Gly Ile Pro Val Gly Thr Ser Gln Ala Gly Gly

260 265 270

Gly Val Leu Ala Trp Asp His Ala Gln Asn Leu Gly Gly Val Gly Ala

275 280 285

Thr Gly Thr Leu Ala Ala Asn Arg Ile Ala Gly Asp Ala Asp Val Ile

290 295 300

Ile Gly Ile Gly Thr Arg Tyr Ser Asp Phe Thr Thr Ala Ser Arg Thr

305 310 315 320

Ala Phe Gln Asn Pro Asp Val Thr Phe Ile Asn Ile Asn Val Ala Ser

325 330 335

Phe Asp Ala Tyr Lys His Gly Thr Gln Leu Pro Val Ile Ala Asp Ala

340 345 350

Arg Glu Ala Ile Val Glu Leu Ala Glu Ala Leu Gln Gly Phe Thr Val

355 360 365

Ala Glu Asp Tyr Ala Gln Arg Ile Ala Lys Glu Lys Ala Ala Trp Asp

370 375 380

Ala Glu Val Asp Lys Ser Phe Ala Pro Ser Gly Leu Ala Leu Pro Gly

385 390 395 400

Gln Pro Glu Ile Ile Gly Ala Val Gln Ala Ser Thr Ser Glu Lys Asp

405 410 415

Val Ile Val Gln Ala Ala Gly Ser Leu Pro Gly Asp Leu His Lys Leu

420 425 430

Trp Arg Val Arg Asp Ala Leu Gly Tyr His Val Glu Tyr Ala Phe Ser

435 440 445

Cys Met Gly Tyr Glu Ile Ala Gly Gly Ile Gly Ala Lys Arg Gly Leu

450 455 460

Asp Ala Ala Gly Asp Asp Arg Asp Val Val Ile Met Val Gly Asp Gly

465 470 475 480

Ser Tyr Leu Met Leu Asn Thr Glu Leu Val Thr Ala Val Ala Glu Gly

485 490 495

Ile Lys Val Ile Val Val Leu Ile Gln Asn His Gly Tyr Ala Ser Ile

500 505 510

Gly His Leu Ser Glu Thr Val Gly Ser Gln Arg Phe Gly Thr Trp Tyr

515 520 525

Arg Glu Tyr Asp Ala Glu Ala Lys Asn Phe Gln Gly Glu Gln Ile Leu

530 535 540

Pro Val Asp Leu Ala Met Asn Ala Arg Ser Tyr Gly Met Asp Val Ile

545 550 555 560

Glu Val Glu Pro Ser Ala Asn Ala Ile Glu Asp Leu Lys Ala Ala Met

565 570 575

Ala Thr Ala Lys Ala Ser Glu Lys Ser Thr Phe Ile His Ile Asn Ser

580 585 590

Asp Pro Leu Ile Tyr Ala Pro Asp Gly Ala Gly Trp Trp Asp Val Pro

595 600 605

Val Ser Glu Thr Ser Thr Leu Asp Ser Thr Asn Ala Ala Arg Glu Asp

610 615 620

Tyr Leu Lys Asn Gln Ala Leu Gln Arg Pro Leu Leu Gly

625 630 635

<210>31

<211>1606

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1576)

<223>RXA00999

<400>31

cggcctaaaa tggttggatt ttcacctcct gtgacctggt aaaatcgcca ctacccccaa 60

atggtcacac cttttaggcc gattttgctg acaccgggct atg ccg tca agt acg 115

Met Pro Ser Ser Thr

1 5

atc aat aac atg act aat gga gat aat ctc gca cag atc ggc gtt gta 163

Ile Asn Asn Met Thr Asn Gly Asp Asn Leu Ala Gln Ile Gly Val Val

10 15 20

ggc cta gca gta atg ggc tca aac ctc gcc cgc aac ttc gcc cgc aac 211

Gly Leu Ala Val Met Gly Ser Asn Leu Ala Arg Asn Phe Ala Arg Asn

25 30 35

ggc aac act gtc gct gtc tac aac cgc agc act gac aaa acc gac aag 259

Gly Asn Thr Val Ala Val Tyr Asn Arg Ser Thr Asp Lys Thr Asp Lys

40 45 50

ctc atc gcc gat cac ggc tcc gaa ggc aac ttc atc cct tct gca acc 307

Leu Ile Ala Asp His Gly Ser Glu Gly Asn Phe Ile Pro Ser Ala Thr

55 60 65

gtc gaa gag ttc gta gca tcc ctg gaa aag cca cgc cgc gcc atc atc 355

Val Glu Glu Phe Val Ala Ser Leu Glu Lys Pro Arg Arg Ala Ile Ile

70 75 80 85

atg gtt cag gct ggt aac gcc acc gac gca gtc atc aac cag ctg gca 403

Met Val Gln Ala Gly Asn Ala Thr Asp Ala Val Ile Asn Gln Leu Ala

90 95 100

gat gcc atg gac gaa ggc gac atc atc atc gac ggc ggc aac gcc ctc 451

Asp Ala Met Asp Glu Gly Asp Ile Ile Ile Asp Gly Gly Asn Ala Leu

105 110 115

tac acc gac acc att cgt cgc gag aag gaa atc tcc gca cgc ggt ctc 499

Tyr Thr Asp Thr Ile Arg Arg Glu Lys Glu Ile Ser Ala Arg Gly Leu

120 125 130

cacttc gtc ggt gct ggt atc tcc ggc ggc gaa gaa ggc gca ctc aac 547

His Phe Val Gly Ala Gly Ile Ser Gly Gly Glu Glu Gly Ala Leu Asn

135 140 145

ggc cca tcc atc atg cct ggt ggc cca gca aag tcc tac gag tcc ctc 595

Gly Pro Ser Ile Met Pro Gly Gly Pro Ala Lys Ser Tyr Glu Ser Leu

150 155 160 165

gga cca ctg ctt gag tcc atc gct gcc aac gtt gac ggc acc cca tgt 643

Gly Pro Leu Leu Glu Ser Ile Ala Ala Asn Val Asp Gly Thr Pro Cys

170 175 180

gtc acc cac atc ggc cca gac ggc gcc ggc cacttc gtc aag atg gtc 691

Val Thr His Ile Gly Pro Asp Gly Ala Gly His Phe Val Lys Met Val

185 190 195

cac aac ggc atc gag tac gcc gac atg cag gtc atc ggc gag gca tac 739

His Asn Gly Ile Glu Tyr Ala Asp Met Gln Val Ile Gly Glu Ala Tyr

200 205 210

cac ctt ctc cgc tac gca gca ggc atg cag cca gct gaa atc gct gag 787

His Leu Leu Arg Tyr Ala Ala Gly Met Gln Pro Ala Glu Ile Ala Glu

215 220 225

gtt ttc aag gaa tgg aac gca ggc gac ctg gat tcc tac ctc atc gaa 835

Val Phe Lys Glu Trp Asn Ala Gly Asp Leu Asp Ser Tyr Leu Ile Glu

230 235 240 245

atc acc gca gag gtt ctc tcc cag gtg gat gct gaa acc ggc aag cca 883

Ile Thr Ala Glu Val Leu Ser Gln Val Asp Ala Glu Thr Gly Lys Pro

250 255 260

cta atc gac gtc atc gtt gac gct gca ggt cag aag ggc acc gga cgt 931

Leu Ile Asp Val Ile Val Asp Ala Ala Gly Gln Lys Gly Thr Gly Arg

265 270 275

tgg acc gtc aag gct gct ctt gat ctg ggt att gct acc acc ggc atc 979

Trp Thr Val Lys Ala Ala Leu Asp Leu Gly Ile Ala Thr Thr Gly Ile

280 285 290

ggc gaa gct gtt ttc gca cgt gca ctc tcc ggc gca acc agc cag cgc 1027

Gly Glu Ala Val Phe Ala Arg Ala Leu Ser Gly Ala Thr Ser Gln Arg

295 300 305

gct gca gca cag ggc aac cta cct gca ggt gtc ctc acc gat ctg gaa 1075

Ala Ala Ala Gln Gly Asn Leu Pro Ala Gly Val Leu Thr Asp Leu Glu

310 315 320 325

gca ctt ggc gtg gac aag gca cag ttc gtc gaa gac gtt cgc cgt gca 1123

Ala Leu Gly Val Asp Lys Ala Gln Phe Val Glu Asp Val Arg Arg Ala

330 335 340

ctg tac gca tcc aag ctt gtt gct tac gca cag ggc ttc gac gag atc 1171

Leu Tyr Ala Ser Lys Leu Val Ala Tyr Ala Gln Gly Phe Asp Glu Ile

345 350 355

aag gct ggc tcc gac gag aac aac tgg gac gtt gac cct cgc gac ctc 1219

Lys Ala Gly Ser Asp Glu Asn Asn Trp Asp Val Asp Pro Arg Asp Leu

360 365 370

gct acc atc tgg cgc ggc ggc tgc atc att cgc gct aag ttc ctc aac 1267

Ala Thr Ile Trp Arg Gly Gly Cys Ile Ile Arg Ala Lys Phe Leu Asn

375 380 385

cgc atc gtc gaa gca tac gat gca aac gct gaa ctt gag tcc ctg ctg 1315

Arg Ile Val Glu Ala Tyr Asp Ala Asn Ala Glu Leu Glu Ser Leu Leu

390 395 400 405

ctc gat cct tac ttc aag agc gag ctc ggc gac ctc atc gat tca tgg 1363

Leu Asp Pro Tyr Phe Lys Ser Glu Leu Gly Asp Leu Ile Asp Ser Trp

410 415 420

cgt cgc gtg att gtc acc gcc acc cag ctt ggc ctg cca atc cca gtg 1411

Arg Arg Val Ile Val Thr Ala Thr Gln Leu Gly Leu Pro Ile Pro Val

425 430 435

ttc gct tcc tcc ctg tcc tac tac gac agc ctg cgt gca gag cgt ctg 1459

Phe Ala Ser Ser Leu Ser Tyr Tyr Asp Ser Leu Arg Ala Glu Arg Leu

440 445 450

cca gca gcc ctg atc caa gga cag cgc gac ttc ttc ggt gcg cac acc 1507

Pro Ala Ala Leu Ile Gln Gly Gln Arg Asp Phe Phe Gly Ala His Thr

455 460 465

tac aag cgc atc gac aag gat ggc tcc ttc cac acc gag tgg tcc ggc 1555

Tyr Lys Arg Ile Asp Lys Asp Gly Ser Phe His Thr Glu Trp Ser Gly

470 475 480 485

gac cgc tcc gag gtt gaa gct taaaggctct ccttttaaca caacgccaaa 1606

Asp Arg Ser Glu Val Glu Ala

490

<210>32

<211>492

<212>PRT

<213>谷氨酸棒状杆菌

<400>32

Met Pro Ser Ser Thr Ile Asn Asn Met Thr Asn Gly Asp Asn Leu Ala

1 5 10 15

Gln Ile Gly Val Val Gly Leu Ala Val Met Gly Ser Asn Leu Ala Arg

20 25 30

Asn Phe Ala Arg Asn Gly Asn Thr Val Ala Val Tyr Asn Arg Ser Thr

35 40 45

Asp Lys Thr Asp Lys Leu Ile Ala Asp His Gly Ser Glu Gly Asn Phe

50 55 60

Ile Pro Ser Ala Thr Val Glu Glu Phe Val Ala Ser Leu Glu Lys Pro

65 70 75 80

Arg Arg Ala Ile Ile Met Val Gln Ala Gly Asn Ala Thr Asp Ala Val

85 90 95

Ile Asn Gln Leu Ala Asp Ala Met Asp Glu Gly Asp Ile Ile Ile Asp

100 105 110

Gly Gly Asn Ala Leu Tyr Thr Asp Thr Ile Arg Arg Glu Lys Glu Ile

115 120 125

Ser Ala Arg Gly Leu His Phe Val Gly Ala Gly Ile Ser Gly Gly Glu

130 135 140

Glu Gly Ala Leu Asn Gly Pro Ser Ile Met Pro Gly Gly Pro Ala Lys

145 150 155 160

Ser Tyr Glu Ser Leu Gly Pro Leu Leu Glu Ser Ile Ala Ala Asn Val

165 170 175

Asp Gly Thr Pro Cys Val Thr His Ile Gly Pro Asp Gly Ala Gly His

180 185 190

Phe Val Lys Met Val His Asn Gly Ile Glu Tyr Ala Asp Met Gln Val

195 200 205

Ile Gly Glu Ala Tyr His Leu Leu Arg Tyr Ala Ala Gly Met Gln Pro

210 215 220

Ala Glu Ile Ala Glu Val Phe Lys Glu Trp Asn Ala Gly Asp Leu Asp

225 230 235 240

Ser Tyr Leu Ile Glu Ile Thr Ala Glu Val Leu Ser Gln Val Asp Ala

245 250 255

Glu Thr Gly Lys Pro Leu Ile Asp Val Ile Val Asp Ala Ala Gly Gln

260 265 270

Lys Gly Thr Gly Arg Trp Thr Val Lys Ala Ala Leu Asp Leu Gly Ile

275 280 285

Ala Thr Thr Gly Ile Gly Glu Ala Val Phe Ala Arg Ala Leu Ser Gly

290 295 300

Ala Thr Ser Gln Arg Ala Ala Ala Gln Gly Asn Leu Pro Ala Gly Val

305 310 315 320

Leu Thr Asp Leu Glu Ala Leu Gly Val Asp Lys Ala Gln Phe Val Glu

325 330 335

Asp Val Arg Arg Ala Leu Tyr Ala Ser Lys Leu Val Ala Tyr Ala Gln

340 345 350

Gly Phe Asp Glu Ile Lys Ala Gly Ser Asp Glu Asn Asn Trp Asp Val

355 360 365

Asp Pro Arg Asp Leu Ala Thr Ile Trp Arg Gly Gly Cys Ile Ile Arg

370 375 380

Ala Lys Phe Leu Asn Arg Ile Val Glu Ala Tyr Asp Ala Asn Ala Glu

385 390 395 400

Leu Glu Ser Leu Leu Leu Asp Pro Tyr Phe Lys Ser Glu Leu Gly Asp

405 410 415

Leu Ile Asp Ser Trp Arg Arg Val Ile Val Thr Ala Thr Gln Leu Gly

420 425 430

Leu Pro Ile Pro Val Phe Ala Ser Ser Leu Ser Tyr Tyr Asp Ser Leu

435 440 445

Arg Ala Glu Arg Leu Pro Ala Ala Leu Ile Gln Gly Gln Arg Asp Phe

450 455 460

Phe Gly Ala His Thr Tyr Lys Arg Ile Asp Lys Asp Gly Ser Phe His

465 470 475 480

Thr Glu Trp Ser Gly Asp Arg Ser Glu Val Glu Ala

485 490

<210>33

<211>739

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(709)

<223>RXA01017

<400>33

gaaatttgag ggggcgctac ccttagaagg tgcgcaatga caccacgata gttcgcgcct 60

agtgtggatt gctagaaaac tttaagaaag aggaaataat atg gct caa aaa gta 115

Met Ala Gln Lys Val

1 5

acc ttc tgg ttc gat acc acc tgc cca ttc tgc tgg gtc acc tcc cgc 163

Thr Phe Trp Phe Asp Thr Thr Cys Pro Phe Cys Trp Val Thr Ser Arg

10 15 20

tgg att aag gaa gtc gaa caa gtc cgc gat att gaa atc cag tgg gtt 211

Trp Ile Lys Glu Val Glu Gln Val Arg Asp Ile Glu Ile Gln Trp Val

25 30 35

cca atg agc ctc gct gtc cta aac gaa ggc cgt gat ctc cca gag gat 259

Pro Met Ser Leu Ala Val Leu Asn Glu Gly Arg Asp Leu Pro Glu Asp

40 45 50

tac aag gag cgc atg aag gct gca tgg gga cca gca cgc gtt ttc gca 307

Tyr Lys Glu Arg Met Lys Ala Ala Trp Gly Pro Ala Arg Val Phe Ala

55 60 65

gct gtc gcc acc gac cat gct gac aag ctc ggc gac ctg tac acc gca 355

Ala Val Ala Thr Asp His Ala Asp Lys Leu Gly Asp Leu Tyr Thr Ala

70 75 80 85

atg ggt acc cgc atc cac aac gac ggt cgc gga cca atc gaa ggt tcc 403

Met Gly Thr Arg Ile His Asn Asp Gly Arg Gly Pro Ile Glu Gly Ser

90 95 100

ttc aat gat gtc atc gca gag gca ctt gaa gag gtc ggc cta gac gct 451

Phe Asn Asp Val Ile Ala Glu Ala Leu Glu Glu Val Gly Leu Asp Ala

105 110 115

gca ctt ggt gaa gtt gca gac acc acc gaa tgg gac gac gca ctt cgc 499

Ala Leu Gly Glu Val Ala Asp Thr Thr Glu Trp Asp Asp Ala Leu Arg

120 125 130

gca ttc cac cag acc gca atg gac gag gtc ggc aac gat gtc gga acc 547

Ala Phe His Gln Thr Ala Met Asp Glu Val Gly Asn Asp Val Gly Thr

135 140 145

cca gtg gtc aag ctc ggc gac acc gct ttc ttc ggc cca gtg ctc acc 595

Pro Val Val Lys Leu Gly Asp Thr Ala Phe Phe Gly Pro Val Leu Thr

150 155 160 165

cgc atc cca cgc ggc gag gaa gca gga gag atc ttc gac gct tcc ttc 643

Arg Ile Pro Arg Gly Glu Glu Ala Gly Glu Ile Phe Asp Ala Ser Phe

170 175 180

aag ctc gca agc tat ccc cac ttc ttt gaa atc aag cgc agc cgc act 691

Lys Leu Ala Ser Tyr Pro His Phe Phe Glu Ile Lys Arg Ser Arg Thr

185 190 195

gag aac cca cag ttc gac taattaacgc tgtctctgct tattaaacac 739

Glu Asn Pro Gln Phe Asp

200

<210>34

<211>203

<212>PRT

<213>谷氨酸棒状杆菌

<400>34

Met Ala Gln Lys Val Thr Phe Trp Phe Asp Thr Thr Cys Pro Phe Cys

1 5 10 15

Trp Val Thr Ser Arg Trp Ile Lys Glu Val Glu Gln Val Arg Asp Ile

20 25 30

Glu Ile Gln Trp Val Pro Met Ser Leu Ala Val Leu Asn Glu Gly Arg

35 40 45

Asp Leu Pro Glu Asp Tyr Lys Glu Arg Met Lys Ala Ala Trp Gly Pro

50 55 60

Ala Arg Val Phe Ala Ala Val Ala Thr Asp His Ala Asp Lys Leu Gly

65 70 75 80

Asp Leu Tyr Thr Ala Met Gly Thr Arg Ile His Asn Asp Gly Arg Gly

85 90 95

Pro Ile Glu Gly Ser Phe Asn Asp Val Ile Ala Glu Ala Leu Glu Glu

100 105 110

Val Gly Leu Asp Ala Ala Leu Gly Glu Val Ala Asp Thr Thr Glu Trp

115 120 125

Asp Asp Ala Leu Arg Ala Phe His Gln Thr Ala Met Asp Glu Val Gly

130 135 140

Asn Asp Val Gly Thr Pro Val Val Lys Leu Gly Asp Thr Ala Phe Phe

145 150 155 160

Gly Pro Val Leu Thr Arg Ile Pro Arg Gly Glu Glu Ala Gly Glu Ile

165 170 175

Phe Asp Ala Ser Phe Lys Leu Ala Ser Tyr Pro His Phe Phe Glu Ile

180 185 190

Lys Arg Ser Arg Thr Glu Asn Pro Gln Phe Asp

195 200

<210>35

<211>1126

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1096)

<223>RXA01025

<400>35

gggcagcagc ggcaggtttc caggaggttt ccatgcgggt ggcttgggac atgggctaac 60

ctgagacggt taaatatcgt tttcgaaagg tgggtttcgc gtg gtt tct gta agc 115

Val Val Ser Val Ser

1 5

gtg atg ggt gca ggt tcc tgg gga acc acg ttg gcc aag gtc ttc tct 163

Val Met Gly Ala Gly Ser Trp Gly Thr Thr Leu Ala Lys Val Phe Ser

10 15 20

gat gct ggc aac gct gtg acg ttg tgg gcg agg cgg gaa gag ttg gca 211

Asp Ala Gly Asn Ala Val Thr Leu Trp Ala Arg Arg Glu Glu Leu Ala

25 30 35

agc acc atc cgt gac agc cat gaa aac cgt gat tac ctt ccg ggg att 259

Ser Thr Ile Arg Asp Ser His Glu Asn Arg Asp Tyr Leu Pro Gly Ile

40 45 50

acg ttg ccg gag tcg ctg cag gtc aca tca tcg gca acg gag gct tta 307

Thr Leu Pro Glu Ser Leu Gln Val Thr Ser Ser Ala Thr Glu Ala Leu

55 60 65

gag ggc gca gcc att gtg gtg ttg gcg att cct tcg cag gcg ttg cgt 355

Glu Gly Ala Ala Ile Val Val Leu Ala Ile Pro Ser Gln Ala Leu Arg

70 75 80 85

ggc aat ttg gcg gag tgg aaa gag acg atc ccg cag gat gcg acc ttg 403

Gly Asn Leu Ala Glu Trp Lys Glu Thr Ile Pro Gln Asp Ala Thr Leu

90 95 100

gtg tcc ttg gct aaa ggt att gaa aag ggc acg cac ctg cgg atg agt 451

Val Ser Leu Ala Lys Gly Ile Glu Lys Gly Thr His Leu Arg Met Ser

105 110 115

gaa gtg atc gcg gag gtg acg gaa gcg gat cct tca cgc atc gcg gtg 499

Glu Val Ile Ala Glu Val Thr Glu Ala Asp Pro Ser Arg Ile Ala Val

120 125 130

ttg tcg ggg cca aac ctt gct cgt gag atc gcg gag ggg cag cct gca 547

Leu Ser Gly Pro Asn Leu Ala Arg Glu Ile Ala Glu Gly Gln Pro Ala

135 140 145

gct acg gtg att gct tgc cct gat gaa aac cga gcg aaa ctt gtg cag 595

Ala Thr Val Ile Ala Cys Pro Asp Glu Asn Arg Ala Lys Leu Val Gln

150 155 160 165

gct gca gtg gct gcg ccg tat ttc cgc ccg tac acc aac act gat gtg 643

Ala Ala Val Ala Ala Pro Tyr Phe Arg Pro Tyr Thr Asn Thr Asp Val

170 175 180

gtg ggc act gaa atc ggt ggt gcg tgt aag aac gtc atc gcg ctg gcc 691

Val Gly Thr Glu Ile Gly Gly Ala Cys Lys Asn Val Ile Ala Leu Ala

185 190 195

tgt ggt att tcc cat ggt tac ggc ctg ggt gag aac acc aat gca tcg 739

Cys Gly Ile Ser His Gly Tyr Gly Leu Gly Glu Asn Thr Asn Ala Ser

200 205 210

ttg att act cgt ggc ctt gca gag atc gca cgc ctc ggt gcc aca ttg 787

Leu Ile Thr Arg Gly Leu Ala Glu Ile Ala Arg Leu Gly Ala Thr Leu

215 220 225

ggt gcg gat gcg aag act ttt tct ggc ctt gcg gga atg ggc gac ttg 835

Gly Ala Asp Ala Lys Thr Phe Ser Gly Leu Ala Gly Met Gly Asp Leu

230 235 240 245

gtg gct acg tgt tca tca ccg ctg tcg cgt aac cgc agc ttc ggt gag 883

Val Ala Thr Cys Ser Ser Pro Leu Ser Arg Asn Arg Ser Phe Gly Glu

250 255 260

cgt ttg ggt cag ggt gaa tcc cta gag aag gct cgc gag gca acc aat 931

Arg Leu Gly Gln Gly Glu Ser Leu Glu Lys Ala Arg Glu Ala Thr Asn

265 270 275

ggt cag gtt gcg gag ggt gtt att tcc tcg cag tcg att ttt gat ctt 979

Gly Gln Val Ala Glu Gly Val Ile Ser Ser Gln Ser Ile Phe Asp Leu

280 285 290

gcc acc aag ctt ggt gtg gag atg ccg atc acc cag gct gtc tac ggt 1027

Ala Thr Lys Leu Gly Val Glu Met Pro Ile Thr Gln Ala Val Tyr Gly

295 300 305

gtg tgc cac cga gat atg aaa gta act gac atg att gtg gct ctc atg 1075

Val Cys His Arg Asp Met Lys Val Thr Asp Met Ile Val Ala Leu Met

310 315 320 325

ggc agg tct aag aag gct gag tagtcttagg ttgtaagctt caatgctgtg 1126

Gly Arg Ser Lys Lys Ala Glu

330

<210>36

<211>332

<212>PRT

<213>谷氨酸棒状杆菌

<400>36

Val Val Ser Val Ser Val Met Gly Ala Gly Ser Trp Gly Thr Thr Leu

1 5 10 15

Ala Lys Val Phe Ser Asp Ala Gly Asn Ala Val Thr Leu Trp Ala Arg

20 25 30

Arg Glu Glu Leu Ala Ser Thr Ile Arg Asp Ser His Glu Asn Arg Asp

35 40 45

Tyr Leu Pro Gly Ile Thr Leu Pro Glu Ser Leu Gln Val Thr Ser Ser

50 55 60

Ala Thr Glu Ala Leu Glu Gly Ala Ala Ile Val Val Leu Ala Ile Pro

65 70 75 80

Ser Gln Ala Leu Arg Gly Asn Leu Ala Glu Trp Lys Glu Thr Ile Pro

85 90 95

Gln Asp Ala Thr Leu Val Ser Leu Ala Lys Gly Ile Glu Lys Gly Thr

100 105 110

His Leu Arg Met Ser Glu Val Ile Ala Glu Val Thr Glu Ala Asp Pro

115 120 125

Ser Arg Ile Ala Val Leu Ser Gly Pro Asn Leu Ala Arg Glu Ile Ala

130 135 140

Glu Gly Gln Pro Ala Ala Thr Val Ile Ala Cys Pro Asp Glu Asn Arg

145 150 155 160

Ala Lys Leu Val Gln Ala Ala Val Ala Ala Pro Tyr Phe Arg Pro Tyr

165 170 175

Thr Asn Thr Asp Val Val Gly Thr Glu Ile Gly Gly Ala Cys Lys Asn

180 185 190

Val Ile Ala Leu Ala Cys Gly Ile Ser His Gly Tyr Gly Leu Gly Glu

195 200 205

Asn Thr Asn Ala Ser Leu Ile Thr Arg Gly Leu Ala Glu Ile Ala Arg

210 215 220

Leu Gly Ala Thr Leu Gly Ala Asp Ala Lys Thr Phe Ser Gly Leu Ala

225 230 235 240

Gly Met Gly Asp Leu Val Ala Thr Cys Ser Ser Pro Leu Ser Arg Asn

245 250 255

Arg Ser Phe Gly Glu Arg Leu Gly Gln Gly Glu Ser Leu Glu Lys Ala

260 265 270

Arg Glu Ala Thr Asn Gly Gln Val Ala Glu Gly Val Ile Ser Ser Gln

275 280 285

Ser Ile Phe Asp Leu Ala Thr Lys Leu Gly Val Glu Met Pro Ile Thr

290 295 300

Gln Ala Val Tyr Gly Val Cys His Arg Asp Met Lys Val Thr Asp Met

305 310 315 320

Ile Val Ala Leu Met Gly Arg Ser Lys Lys Ala Glu

325 330

<210>37

<211>1528

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1498)

<223>RXA01175

<400>37

gggtaaaagg gatcgcgtct cttagtcgat ctgctcccat tccataggtt gggagaaaac 60

ttaactgtta ttccattcct taaaacaccg atatcgtgct atg aat agg ggt gtg 115

Met Asn Arg Gly Val

1 5

agt tgg aca gtt gat atc cct aaa gaa gtt ctc cct gat ttg cca cca 163

Ser Trp Thr Val Asp Ile Pro Lys Glu Val Leu Pro Asp Leu Pro Pro

10 15 20

ttg cca gaa ggc atg cag cag cag ttc gag gac acc att tcc cgt gac 211

Leu Pro Glu Gly Met Gln Gln Gln Phe Glu Asp Thr Ile Ser Arg Asp

25 30 35

gct aag cag caa cct acg tgg gat cgt gca cag gca gaa aac gtg cgc 259

Ala Lys Gln Gln Pro Thr Trp Asp Arg Ala Gln Ala Glu Asn Val Arg

40 45 50

aag atc ctt gag tcg gtt cct cca atc gtt gtt gcc cct gag gta ctt 307

Lys Ile Leu Glu Ser Val Pro Pro Ile Val Val Ala Pro Glu Val Leu

55 60 65

gag ctg aag cag aag ctt gct gat gtt gcc aac ggt aag gcc ttc ctc 355

Glu Leu Lys Gln Lys Leu Ala Asp Val Ala Asn Gly Lys Ala Phe Leu

70 75 80 85

ttg cag ggt ggt gac tgt gcg gaa act ttc gag tca aac act gag ccg 403

Leu Gln Gly Gly Asp Cys Ala Glu Thr Phe Glu Ser Asn Thr Glu Pro

90 95 100

cac att cgc gcc aac gta aag act ctg ctg cag atg gca gtt gtt ttg 451

His Ile Arg Ala Asn Val Lys Thr Leu Leu Gln Met Ala Val Val Leu

105 110 115

acc tac ggt gca tcc act cct gtg atc aag atg gct cgt att gct ggt 499

Thr Tyr Gly Ala Ser Thr Pro Val Ile Lys Met Ala Arg Ile Ala Gly

120 125 130

cag tac gca aag cct cgc tct tct gat ctg gat gga aat ggt ctg cca 547

Gln Tyr Ala Lys Pro Arg Ser Ser Asp Leu Asp Gly Asn Gly Leu Pro

135 140 145

aac tac cgt ggc gat atc gtc aac ggt gtg gag gca acc cca gag gct 595

Asn Tyr Arg Gly Asp Ile Val Asn Gly Val Glu Ala Thr Pro Glu Ala

150 155 160 165

cgt cgc cac gat cct gcc cgc atg atc cgt gct tac gct aac gct tct 643

Arg Arg His Asp Pro Ala Arg Met Ile Arg Ala Tyr Ala Asn Ala Ser

170 175 180

gct gcg atg aac ttg gtg cgc gcg ctc acc agc tct ggc acc gct gat 691

Ala Ala Met Asn Leu Val Arg Ala Leu Thr Ser Ser Gly Thr Ala Asp

185 190 195

ctt tac cgt ctc agc gag tgg aac cgc gag ttc gtt gcg aac tcc cca 739

Leu Tyr Arg Leu Ser Glu Trp Asn Arg Glu Phe Val Ala Asn Ser Pro

200 205 210

gct ggt gca cgc tac gag gct ctt gct cgt gag atc gac tcc ggt ctg 787

Ala Gly Ala Arg Tyr Glu Ala Leu Ala Arg Glu Ile Asp Ser Gly Leu

215 220 225

cgc ttc atg gaa gca tgt ggc gtg tcc gat gag tcc ctg cgt gct gca 835

Arg Phe Met Glu Ala Cys Gly Val Ser Asp Glu Ser Leu Arg Ala Ala

230 235 240 245

gat atc tac tgc tcc cac gag gct ttg ctg gtg gat tac gag cgt tcc 883

Asp Ile Tyr Cys Ser His Glu Ala Leu Leu Val Asp Tyr Glu Arg Ser

250 255 260

atg ctg cgt ctt gca acc gat gag gaa ggc aac gag gaa ctt tac gat 931

Met Leu Arg Leu Ala Thr Asp Glu Glu Gly Asn Glu Glu Leu Tyr Asp

265 270 275

ctt tca gct cac cag ctg tgg atc ggc gag cgc acc cgt ggc atg gat 979

Leu Ser Ala His Gln Leu Trp Ile Gly Glu Arg Thr Arg Gly Met Asp

280 285 290

gat ttc cat gtg aac ttc gca tcc atg atc tct aac cca atc ggc atc 1027

Asp Phe His Val Asn Phe Ala Ser Met Ile Ser Asn Pro Ile Gly Ile

295 300 305

aag att ggt cct ggt atc acc cct gaa gag gct gtt gca Lac gct gac 1075

Lys Ile Gly Pro Gly Ile Thr Pro Glu Glu Ala Val Ala Tyr Ala Asp

310 315 320 325

aag ctc gat ccg aac ttc gag cct ggc cgt ttg acc atc gtt gct cgc 1123

Lys Leu Asp Pro Asn Phe Glu Pro Gly Arg Leu Thr Ile Val Ala Arg

330 335 340

atg ggc cac gac aag gtt cgc tcc gta ctt cct ggt gtt atc cag gct 1171

Met Gly His Asp Lys Val Arg Ser Val Leu Pro Gly Val Ile Gln Ala

345 350 355

gtt gag gca tcc gga cac aag gtt att tgg cag tcc gat ccg atg cac 1219

Val Glu Ala Ser Gly His Lys Val Ile Trp Gln Ser Asp Pro Met His

360 365 370

ggc aac act ttc acc gca tcc aat ggc tac aag acc cgt cacttc gac 1267

Gly Asn Thr Phe Thr Ala Ser Asn Gly Tyr Lys Thr Arg His Phe Asp

375 380 385

aag gtt atc gat gag gtc cag ggc ttc ttc gag gtc cac cgc gca ttg 1315

Lys Val Ile Asp Glu Val Gln Gly Phe Phe Glu Val His Arg Ala Leu

390 395 400 405

ggc acc cac cca ggc gga atc cac att gag ttc act ggt gaa gat gtc 1363

Gly Thr His Pro Gly Gly Ile His Ile Glu Phe Thr Gly Glu Asp Val

410 415 420

acc gag tgc ctc ggt ggc gct gaa gac atc acc gat gtt gat ctg cca 1411

Thr Glu Cys Leu Gly Gly Ala Glu Asp Ile Thr Asp Val Asp Leu Pro

425 430 435

ggc cgc tac gag tcc gca tgc gat cct cgc ctg aac act cag cag tct 1459

Gly Arg Tyr Glu Ser Ala Cys Asp Pro Arg Leu Asn Thr Gln Gln Ser

440 445 450

ttg gag ttg gct ttc ctc gtt gca gaa atg ctg cgt aac taagctttta 1508

Leu Glu Leu Ala Phe Leu Val Ala Glu Met Leu Arg Asn

455 460 465

gaccaactct aaaaaagcta 1528

<210>38

<211>466

<212>PRT

<213>谷氨酸棒状杆菌

<400>38

Met Asn Arg Gly Val Ser Trp Thr Val Asp Ile Pro Lys Glu Val Leu

1 5 10 15

Pro Asp Leu Pro Pro Leu Pro Glu Gly Met Gln Gln Gln Phe Glu Asp

20 25 30

Thr Ile Ser Arg Asp Ala Lys Gln Gln Pro Thr Trp Asp Arg Ala Gln

35 40 45

Ala Glu Asn Val Arg Lys Ile Leu Glu Ser Val Pro Pro Ile Val Val

50 55 60

Ala Pro Glu Val Leu Glu Leu Lys Gln Lys Leu Ala Asp Val Ala Asn

65 70 75 80

Gly Lys Ala Phe Leu Leu Gln Gly Gly Asp Cys Ala Glu Thr Phe Glu

85 90 95

Ser Asn Thr Glu Pro His Ile Arg Ala Asn Val Lys Thr Leu Leu Gln

100 105 110

Met Ala Val Val Leu Thr Tyr Gly Ala Ser Thr Pro Val Ile Lys Met

115 120 125

Ala Arg Ile Ala Gly Gln Tyr Ala Lys Pro Arg Ser Ser Asp Leu Asp

130 135 140

Gly Asn Gly Leu Pro Asn Tyr Arg Gly Asp Ile Val Asn Gly Val Glu

145 150 155 160

Ala Thr Pro Glu Ala Arg Arg His Asp Pro Ala Arg Met Ile Arg Ala

165 170 175

Tyr Ala Asn Ala Ser Ala Ala Met Asn Leu Val Arg Ala Leu Thr Ser

180 185 190

Ser Gly Thr Ala Asp Leu Tyr Arg Leu Ser Glu Trp Asn Arg Glu Phe

195 200 205

Val Ala Asn Ser Pro Ala Gly Ala Arg Tyr Glu Ala Leu Ala Arg Glu

210 215 220

Ile Asp Ser Gly Leu Arg Phe Met Glu Ala Cys Gly Val Ser Asp Glu

225 230 235 240

Ser Leu Arg Ala Ala Asp Ile Tyr Cys Ser His Glu Ala Leu Leu Val

245 250 255

Asp Tyr Glu Arg Ser Met Leu Arg Leu Ala Thr Asp Glu Glu Gly Asn

260 265 270

Glu Glu Leu Tyr Asp Leu Ser Ala His Gln Leu Trp Ile Gly Glu Arg

275 280 285

Thr Arg Gly Met Asp Asp Phe His Val Asn Phe Ala Ser Met Ile Ser

290 295 300

Asn Pro Ile Gly Ile Lys Ile Gly Pro Gly Ile Thr Pro Glu Glu Ala

305 310 315 320

Val Ala Tyr Ala Asp Lys Leu Asp Pro Asn Phe Glu Pro Gly Arg Leu

325 330 335

Thr Ile Val Ala Arg Met Gly His Asp Lys Val Arg Ser Val Leu Pro

340 345 350

Gly Val Ile Gln Ala Val Glu Ala Ser Gly His Lys Val Ile Trp Gln

355 360 365

Ser Asp Pro Met His Gly Asn Thr Phe Thr Ala Ser Asn Gly Tyr Lys

370 375 380

Thr Arg His Phe Asp Lys Val Ile Asp Glu Val Gln Gly Phe Phe Glu

385 390 395 400

Val His Arg Ala Leu Gly Thr His Pro Gly Gly Ile His Ile Glu Phe

405 410 415

Thr Gly Glu Asp Val Thr Glu Cys Leu Gly Gly Ala Glu Asp Ile Thr

420 425 430

Asp Val Asp Leu Pro Gly Arg Tyr Glu Ser Ala Cys Asp Pro Arg Leu

435 440 445

Asn Thr Gln Gln Ser Leu Glu Leu Ala Phe Leu Val Ala Glu Met Leu

450 455 460

Arg Asn

465

<210>39

<211>1237

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1207)

<223>RXA01350

<400>39

agctgacaac tataaatggt gcccgatatc aggaaaaatt gcttgcacac cgcgccgatt 60

ccccatgatg ccctaacatc ttgcaggtga ggggtacata ttg ggg caa ttc ggg 115

Leu Gly Gln Phe Gly

1 5

ggt aat ttt gca gta tcg tca aga tca ccc aaa act ggt ggc tgt tct 163

Gly Asn Phe Ala Val Ser Ser Arg Ser Pro Lys Thr Gly Gly Cys Ser

10 15 20

ctt tta agc ggg ata gca tgg gtt ctt aga gga ccc cct aca agg att 211

Leu Leu Ser Gly Ile Ala Trp Val Leu Arg Gly Pro Pro Thr Arg Ile

25 30 35

gag gat tgt tta atg aat tcc ccg cag aac gtc tcc acc aag aag gtc 259

Glu Asp Cys Leu Met Asn Ser Pro Gln Asn Val Ser Thr Lys Lys Val

40 45 50

acc gtc acc ggc gca gct ggt caa atc tct tat tca ctg ttg tgg cgc 307

Thr Val Thr Gly Ala Ala Gly Gln Ile Ser Tyr Ser Leu Leu Trp Arg

55 60 65

atc gcc aac ggt gaa gta ttc ggc acc gac acc cct gta gaa ctg aaa 355

Ile Ala Asn Gly Glu Val Phe Gly Thr Asp Thr Pro Val Glu Leu Lys

70 75 80 85

ctt ctg gag atc cct cag gct ctt ggc ggg gca gag ggt gtg gct atg 403

Leu Leu Glu Ile Pro Gln Ala Leu Gly Gly Ala Glu Gly Val Ala Met

90 95 100

gaa ctt ctg gat tct gcc ttc ccc ctc ctg cga aac atc acc atc acc 451

Glu Leu Leu Asp Ser Ala Phe Pro Leu Leu Arg Asn Ile Thr Ile Thr

105 110 115

gcg gat gcc aat gag gca ttc gac ggc gct aat gcg gcg ttt ttg gtc 499

Ala Asp Ala Asn Glu Ala Phe Asp Gly Ala Asn Ala Ala Phe Leu Val

120 125 130

ggt gcg aag cct cgc gga aaa ggc gaa gag cgc gca gat ttg ctg gct 547

Gly Ala Lys Pro Arg Gly Lys Gly Glu Glu Arg Ala Asp Leu Leu Ala

135 140 145

aac aac ggc aag att ttc gga cct caa ggt aaa gct atc aat gac aac 595

Asn Asn Gly Lys Ile Phe Gly Pro Gln Gly Lys Ala Ile Asn Asp Asn

150 155 160 165

gcc gca gat gac att cgt gtc cta gtt gtt gga aac cca gcg aac acc 643

Ala Ala Asp Asp Ile Arg Val Leu Val Val Gly Asn Pro Ala Asn Thr

170 175 180

aac gcg ttg att gct tca gct gcg gcc cca gat gtt cca gca tcc cgc 691

Asn Ala Leu Ile Ala Ser Ala Ala Ala Pro Asp Val Pro Ala Ser Arg

185 190 195

ttc aac gca atg atg cgc ctt gat cac aac cgt gcg atc tcc cag ctg 739

Phe Asn Ala Met Met Arg Leu Asp His Asn Arg Ala Ile Ser Gln Leu

200 205 210

gcc acc aag ctt ggc cgt gga tct gcg gaa ttt aac aac att gtg gtc 787

Ala Thr Lys Leu Gly Arg Gly Ser Ala Glu Phe Asn Asn Ile Val Val

215 220 225

tgg gga aat cac tcc gca acc cag ttc cca gac atc acc tac gca acc 835

Trp Gly Asn His Ser Ala Thr Gln Phe Pro Asp Ile Thr Tyr Ala Thr

230 235 240 245

gtt ggt gga gaa aag gtc act gac ctg gtt gat cac gat tgg tat gtg 883

Val Gly Gly Glu Lys Val Thr Asp Leu Val Asp His Asp Trp Tyr Val

250 255 260

gag gag ttc att cct cgc gtg gct aac cgt ggc gct gaa atc att gag 931

Glu Glu Phe Ile Pro Arg Val Ala Asn Arg Gly Ala Glu Ile Ile Glu

265 270 275

gtc cgt gga aag tct tct gca gct tct gca gca tcc tct gcg att gat 979

Val Arg Gly Lys Ser Ser Ala Ala Ser Ala Ala Ser Ser Ala Ile Asp

280 285 290

cac atg cgc gat tgg gta cag ggc acc gag gcg tgg tcc tct gcg gca 1027

His Met Arg Asp Trp Val Gln Gly Thr Glu Ala Trp Ser Ser Ala Ala

295 300 305

att cct tcc acc ggt gca tac ggc att cct gag ggc att ttt gtc ggt 1075

Ile Pro Ser Thr Gly Ala Tyr Gly Ile Pro Glu Gly Ile Phe Val Gly

310 315 320 325

ctg cca acc gta tcc cgc aac ggt gag tgg gaa atc gtt gaa ggc ctg 1123

Leu Pro Thr Val Ser Arg Asn Gly Glu Trp Glu Ile Val Glu Gly Leu

330 335 340

gag att tcc gat ttc cag cgc gcc cgc atc gac gcg aat gct cag gaa 1171

Glu Ile Ser Asp Phe Gln Arg Ala Arg Ile Asp Ala Asn Ala Gln Glu

345 350 355

ttg cag gcc gag cgc gag gca gtg cgc gac ttg ctc taatctttaa 1217

Leu Gln Ala Glu Arg Glu Ala Val Arg Asp Leu Leu

360 365

cgcatgactt cgcttttcga 1237

<210>40

<211>369

<212>PRT

<213>谷氨酸棒状杆菌

<400>40

Leu Gly Gln Phe Gly Gly Asn Phe Ala Val Ser Ser Arg Ser Pro Lys

1 5 10 15

Thr Gly Gly Cys Ser Leu Leu Ser Gly Ile Ala Trp Val Leu Arg Gly

20 25 30

Pro Pro Thr Arg Ile Glu Asp Cys Leu Met Asn Ser Pro Gln Asn Val

35 40 45

Ser Thr Lys Lys Val Thr Val Thr Gly Ala Ala Gly Gln Ile Ser Tyr

50 55 60

Ser Leu Leu Trp Arg Ile Ala Asn Gly Glu Val Phe Gly Thr Asp Thr

65 70 75 80

Pro Val Glu Leu Lys Leu Leu Glu Ile Pro Gln Ala Leu Gly Gly Ala

85 90 95

Glu Gly Val Ala Met Glu Leu Leu Asp Ser Ala Phe Pro Leu Leu Arg

100 105 110

Asn Ile Thr Ile Thr Ala Asp Ala Asn Glu Ala Phe Asp Gly Ala Asn

115 120 125

Ala Ala Phe Leu Val Gly Ala Lys Pro Arg Gly Lys Gly Glu Glu Arg

130 135 140

Ala Asp Leu Leu Ala Asn Asn Gly Lys Ile Phe Gly Pro Gln Gly Lys

145 150 155 160

Ala Ile Asn Asp Asn Ala Ala Asp Asp Ile Arg Val Leu Val Val Gly

165 170 175

Asn Pro Ala Asn Thr Asn Ala Leu Ile Ala Ser Ala Ala Ala Pro Asp

180 185 190

Val Pro Ala Ser Arg Phe Asn Ala Met Met Arg Leu Asp His Asn Arg

195 200 205

Ala Ile Ser Gln Leu Ala Thr Lys Leu Gly Arg Gly Ser Ala Glu Phe

210 215 220

Asn Asn Ile Val Val Trp Gly Asn His Ser Ala Thr Gln Phe Pro Asp

225 230 235 240

Ile Thr Tyr Ala Thr Val Gly Gly Glu Lys Val Thr Asp Leu Val Asp

245 250 255

His Asp Trp Tyr Val Glu Glu Phe Ile Pro Arg Val Ala Asn Arg Gly

260 265 270

Ala Glu Ile Ile Glu Val Arg Gly Lys Ser Ser Ala Ala Ser Ala Ala

275 280 285

Ser Ser Ala Ile Asp His Met Arg Asp Trp Val Gln Gly Thr Glu Ala

290 295 300

Trp Ser Ser Ala Ala Ile Pro Ser Thr Gly Ala Tyr Gly Ile Pro Glu

305 310 315 320

Gly Ile Phe Val Gly Leu Pro Thr Val Ser Arg Asn Gly Glu Trp Glu

325 330 335

Ile Val Glu Gly Leu Glu Ile Ser Asp Phe Gln Arg Ala Arg Ile Asp

340 345 350

Ala Asn Ala Gln Glu Leu Gln Ala Glu Arg Glu Ala Val Arg Asp Leu

355 360 365

Leu

<210>41

<211>1207

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1177)

<223>RXA01392

<400>41

gtctgcaatc accccgaaca tttgttcaat cgttgatttt cattccactt cgtaatattg 60

ttgacatatc atctaaattt ccaagagagg acaccacaca gtg gct aac acg tca 115

Val Ala Asn Thr Ser

1 5

tcc gat tgg gca ggc gcc cca caa aat gca tca gca gac ggc gag ttc 163

Ser Asp Trp Ala Gly Ala Pro Gln Asn Ala Ser Ala Asp Gly Glu Phe

10 15 20

gtt cgc gat acc aac tac atc gat gac cgc atc gtc gca gac gtt cca 211

Val Arg Asp Thr Asn Tyr Ile Asp Asp Arg Ile Val Ala Asp Val Pro

25 30 35

gcg gga tcc gaa cca att gct cag gaa gat ggc act ttc cat tgg cct 259

Ala Gly Ser Glu Pro Ile Ala Gln Glu Asp Gly Thr Phe His Trp Pro

40 45 50

gtc gag gct ggt cgc tac cgt tta gtc gct gcc cgc gca tgt cca tgg 307

Val Glu Ala Gly Arg Tyr Arg Leu Val Ala Ala Arg Ala Cys Pro Trp

55 60 65

gca cac cgc act gtt atc acc cgt cgt ctt ctc ggc ctg gag aac gtg 355

Ala His Arg Thr Val Ile Thr Arg Arg Leu Leu Gly Leu Glu Asn Val

70 75 80 85

atc tcg ctt ggt ctg acc ggc ccg act cac gac gtt cgt tcc tgg act 403

Ile Ser Leu Gly Leu Thr Gly Pro Thr His Asp Val Arg Ser Trp Thr

90 95 100

ttc gat tta gat cca aac cat ctt gat ccc gtg ctg cag att cct cgt 451

Phe Asp Leu Asp Pro Asn His Leu Asp Pro Val Leu Gln Ile Pro Arg

105 110 115

cta cag gac gcg tat ttc aac cgc ttc ccc gat tac ccg cgc ggc att 499

Leu Gln Asp Ala Tyr Phe Asn Arg Phe Pro Asp Tyr Pro Arg Gly Ile

120 125 130

act gtc cca gcg ctc gtg gag gaa tcg tct aag aag gtc gtc acc aac 547

Thr Val Pro Ala Leu Val Glu Glu Ser Ser Lys Lys Val Val Thr Asn

135 140 145

gat tac cct tcc atc acc atc gat ttc aat ctt gag tgg aag cag ttc 595

Asp Tyr Pro Ser Ile Thr Ile Asp Phe Asn Leu Glu Trp Lys Gln Phe

150 155 160 165

cac cgt gag ggt gcg cct aac ctc tac ccc gcg gag ctg cgc gag gag 643

His Arg Glu Gly Ala Pro Asn Leu Tyr Pro Ala Glu Leu Arg Glu Glu

170 175 180

atg gcg ccg gtg atg aag cgc atc ttc act gag gtc aac aac ggc gta 691

Met Ala Pro Val Met Lys Arg Ile Phe Thr Glu Val Asn Asn Gly Val

185 190 195

tac agg acc ggc ttt gcc ggt agc cag gaa gcg cac aac gag gcg tac 739

Tyr Arg Thr Gly Phe Ala Gly Ser Gln Glu Ala His Asn Glu Ala Tyr

200 205 210

aag cgg ctt tgg gtt gcg ttg gac tgg cta gaa gat cgc tta tcg acg 787

Lys Arg Leu Trp Val Ala Leu Asp Trp Leu Glu Asp Arg Leu Ser Thr

215 220 225

cga cgt tac ctc atg ggg gat cac atc acc gag gcg gat atc cgc ctc 835

Arg Arg Tyr Leu Met Gly Asp His Ile Thr Glu Ala Asp Ile Arg Leu

230 235 240 245

tac cca acc ctc gtg cgt ttc gat gcc gtc tac cac gga cac ttc aag 883

Tyr Pro Thr Leu Val Arg Phe Asp Ala Val Tyr His Gly His Phe Lys

250 255 260

tgt ggc cgc aac aag atc acc gaa atg ccg aat cta tgg ggc tac ctg 931

Cys Gly Arg Asn Lys Ile Thr Glu Met Pro Asn Leu Trp Gly Tyr Leu

265 270 275

cgg gat ctt ttc cag acc cca ggc ttt ggc gac acc acc gat ttc acc 979

Arg Asp Leu Phe Gln Thr Pro Gly Phe Gly Asp Thr Thr Asp Phe Thr

280 285 290

gaa atc aag cag cac tac tac atc acc cac gcg gag att aac ccc acc 1027

Glu Ile Lys Gln His Tyr Tyr Ile Thr His Ala Glu Ile Asn Pro Thr

295 300 305

cgg atc gtt cca gtc gga cca gat ctg tct ggt ttc gcg aca cca cac 1075

Arg Ile Val Pro Val Gly Pro Asp Leu Ser Gly Phe Ala Thr Pro His

310 315 320 325

ggc cgt gaa aag ctc ggc gga tcc cca ttt gct gaa ggt gtt act ctg 1123

Gly Arg Glu Lys Leu Gly Gly Ser Pro Phe Ala Glu Gly Val Thr Leu

330 335 340

cct ggc cca att cct gcg ggc gaa gaa gtg aaa aac cct gaa cct ttt 1171

Pro Gly Pro Ile Pro Ala Gly Glu Glu Val Lys Asn Pro Glu Pro Phe

345 350 355

cag aag taactaaggc cgcaatccct cgattgctgc 1207

Gln Lys

<210>42

<211>359

<212>PRT

<213>谷氨酸棒状杆菌

<400>42

Val Ala Asn Thr Ser Ser Asp Trp Ala Gly Ala Pro Gln Asn Ala Ser

1 5 10 15

Ala Asp Gly Glu Phe Val Arg Asp Thr Asn Tyr Ile Asp Asp Arg Ile

20 25 30

Val Ala Asp Val Pro Ala Gly Ser Glu Pro Ile Ala Gln Glu Asp Gly

35 40 45

Thr Phe His Trp Pro Val Glu Ala Gly Arg Tyr Arg Leu Val Ala Ala

50 55 60

Arg Ala Cys Pro Trp Ala His Arg Thr Val Ile Thr Arg Arg Leu Leu

65 70 75 80

Gly Leu Glu Asn Val Ile Ser Leu Gly Leu Thr Gly Pro Thr His Asp

85 90 95

Val Arg Ser Trp Thr Phe Asp Leu Asp Pro Asn His Leu Asp Pro Val

100 105 110

Leu Gln Ile Pro Arg Leu Gln Asp Ala Tyr Phe Asn Arg Phe Pro Asp

115 120 125

Tyr Pro Arg Gly Ile Thr Val Pro Ala Leu Val Glu Glu Ser Ser Lys

130 135 140

Lys Val Val Thr Asn Asp Tyr Pro Ser Ile Thr Ile Asp Phe Asn Leu

145 150 155 160

Glu Trp Lys Gln Phe His Arg Glu Gly Ala Pro Asn Leu Tyr Pro Ala

165 170 175

Glu Leu Arg Glu Glu Met Ala Pro Val Met Lys Arg Ile Phe Thr Glu

180 185 190

Val Asn Asn Gly Val Tyr Arg Thr Gly Phe Ala Gly Ser Gln Glu Ala

195 200 205

His Asn Glu Ala Tyr Lys Arg Leu Trp Val Ala Leu Asp Trp Leu Glu

210 215 220

Asp Arg Leu Ser Thr Arg Arg Tyr Leu Met Gly Asp His Ile Thr Glu

225 230 235 240

Ala Asp Ile Arg Leu Tyr Pro Thr Leu Val Arg Phe Asp Ala Val Tyr

245 250 255

His Gly His Phe Lys Cys Gly Arg Asn Lys Ile Thr Glu Met Pro Asn

260 265 270

Leu Trp Gly Tyr Leu Arg Asp Leu Phe Gln Thr Pro Gly Phe Gly Asp

275 280 285

Thr Thr Asp Phe Thr Glu Ile Lys Gln His Tyr Tyr Ile Thr His Ala

290 295 300

Glu Ile Asn Pro Thr Arg Ile Val Pro Val Gly Pro Asp Leu Ser Gly

305 310 315 320

Phe Ala Thr Pro His Gly Arg Glu Lys Leu Gly Gly Ser Pro Phe Ala

325 330 335

Glu Gly Val Thr Leu Pro Gly Pro Ile Pro Ala Gly Glu Glu Val Lys

340 345 350

Asn Pro Glu Pro Phe Gln Lys

355

<210>43

<211>1321

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1291)

<223>RXA01436

<400>43

gcctaaacaa accagtcaac gacctttccc gtggcgcaac agtccctgac atcgtcaaca 60

cagtagccat cacagcaatt caggcaggag gacgcagcta atg gca ttg gca ctt 115

Met Ala Leu Ala Leu

1 5

gtt ttg aac tcc ggt tca tct tcc atc aaa ttc cag ctg gtc aac ccc 163

Val Leu Asn Ser Gly Ser Ser Ser Ile Lys Phe Gln Leu Val Asn Pro

10 15 20

gaa aac tct gcc atc gac gag cca tat gtt tct ggt ctt gtg gag cag 211

Glu Asn Ser Ala Ile Asp Glu Pro Tyr Val Ser Gly Leu Val Glu Gln

25 30 35

att ggt gag cca aac ggc cgc atc gta ctc aaa ata gag ggt gaa aaa 259

Ile Gly Glu Pro Asn Gly Arg Ile Val Leu Lys Ile Glu Gly Glu Lys

40 45 50

tat acc cta gag aca ccc atc gca gat cac tcc gaa ggc cta aac ctg 307

Tyr Thr Leu Glu Thr Pro Ile Ala Asp His Ser Glu Gly Leu Asn Leu

55 60 65

gcg ttc gat ctc atg gac cag cac aac tgt ggt cct tcc caa ctg gaa 355

Ala Phe Asp Leu Met Asp Gln His Asn Cys Gly Pro Ser Gln Leu Glu

70 75 80 85

atc acc gca gtt gga cac cgc gtg gtc cac ggc gga atc ttg ttc tcc 403

Ile Thr Ala Val Gly His Arg Val Val His Gly Gly Ile Leu Phe Ser

90 95 100

gca ccg gaa ctt atc act gat gaa atc gtg gaa atg atc cgc gat ctc 451

Ala Pro Glu Leu Ile Thr Asp Glu Ile Val Glu Met Ile Arg Asp Leu

105 110 115

att cca ctc gca cca ctg cac aac cct gca aac gtt gac ggc att gat 499

Ile Pro Leu Ala Pro Leu His Asn Pro Ala Asn Val Asp Gly Ile Asp

120 125 130

gtt gct cga aaa att ctc ccc gat gtc cca cac gta gct gtc ttt gac 547

Val Ala Arg Lys Ile Leu Pro Asp Val Pro His Val Ala Val Phe Asp

135 140 145

acc ggt ttc ttc cac tca ctt cca cca gca gct gcg ctg tat gcc atc 595

Thr Gly Phe Phe His Ser Leu Pro Pro Ala Ala Ala Leu Tyr Ala Ile

150 155 160 165

aac aag gat gtc gca gct gaa cac gga atc agg cgc tat ggt ttc cac 643

Asn Lys Asp Val Ala Ala Glu His Gly Ile Arg Arg Tyr Gly Phe His

170 175 180

ggc acc tcc cat gaa ttt gtg tcc aag cgc gtg gtg gaa att ctg gaa 691

Gly Thr Ser His Glu Phe Val Ser Lys Arg Val Val Glu Ile Leu Glu

185 190 195

aag ccc acc gaa gac atc aac acc atc acc ttc cac ctg ggc aac ggc 739

Lys Pro Thr Glu Asp Ile Asn Thr Ile Thr Phe His Leu Gly Asn Gly

200 205 210

gca tcc atg gct gct gtt caa ggt ggc cgt gcg gta gat act tcc atg 787

Ala Ser Met Ala Ala Val Gln Gly Gly Arg Ala Val Asp Thr Ser Met

215 220 225

ggt atg aca cct ctc gcg ggc ctt gtc atg ggt acc cga agc ggt gac 835

Gly Met Thr Pro Leu Ala Gly Leu Val Met Gly Thr Arg Ser Gly Asp

230 235 240 245

att gat cca ggt atc gtc ttc cac ctt tcc cgc acc gct ggc atg agc 883

Ile Asp Pro Gly Ile Val Phe His Leu Ser Arg Thr Ala Gly Met Ser

250 255 260

atc gat gag atc gat aat ctg ctg aac aaa aag tcg ggt gta aag gga 931

Ile Asp Glu Ile Asp Asn Leu Leu Asn Lys Lys Ser Gly Val Lys Gly

265 270 275

ctt tcc ggt gtt aat gat ttc cgt gaa ctg cgg gaa atg atc gac aac 979

Leu Ser Gly Val Asn Asp Phe Arg Glu Leu Arg Glu Met Ile Asp Asn

280 285 290

aat gat caa gat gcc tgg tcc gcg tac aac att tac ata cac caa ctc 1027

Asn Asp Gln Asp Ala Trp Ser Ala Tyr Asn Ile Tyr Ile His Gln Leu

295 300 305

cgc cgc tac ctc ggt tcc tac atg gtg gca ctg gga cgg gta gac acc 1075

Arg Arg Tyr Leu Gly Ser Tyr Met Val Ala Leu Gly Arg Val Asp Thr

310 315 320 325

atc gtg ttc acc gcc ggt gtc ggt gaa aat gcc cag ttt gtc cgt gag 1123

Ile Val Phe Thr Ala Gly Val Gly Glu Asn Ala Gln Phe Val Arg Glu

330 335 340

gat gcc ttg gca ggt ttg gaa atg tac gga att gag atc gat cca gag 1171

Asp Ala Leu Ala Gly Leu Glu Met Tyr Gly Ile Glu Ile Asp Pro Glu

345 350 355

cgt aac gca ttg cca aac gat ggt cct cga ttg att tcc acc gat gcc 1219

Arg Asn Ala Leu Pro Asn Asp Gly Pro Arg Leu Ile Ser Thr Asp Ala

360 365 370

tcc aag gtg aag gtg ttt gtt att cca act aat gaa gag tta gct atc 1267

Ser Lys Val Lys Val Phe Val Ile Pro Thr Asn Glu Glu Leu Ala Ile

375 380 385

gct agg tac gcg gtg aag ttc gct tagctctcct ggttaggatc caccacaaat 1321

Ala Arg Tyr Ala Val Lys Phe Ala

390 395

<210>44

<211>397

<212>PRT

<213>谷氨酸棒状杆菌

<400>44

Met Ala Leu Ala Leu Val Leu Asn Ser Gly Ser Ser Ser Ile Lys Phe

1 5 10 15

Gln Leu Val Asn Pro Glu Asn Ser Ala Ile Asp Glu Pro Tyr Val Ser

20 25 30

Gly Leu Val Glu Gln Ile Gly Glu Pro Asn Gly Arg Ile Val Leu Lys

35 40 45

Ile Glu Gly Glu Lys Tyr Thr Leu Glu Thr Pro Ile Ala Asp His Ser

50 55 60

Glu Gly Leu Asn Leu Ala Phe Asp Leu Met Asp Gln His Asn Cys Gly

65 70 75 80

Pro Ser Gln Leu Glu Ile Thr Ala Val Gly His Arg Val Val His Gly

85 90 95

Gly Ile Leu Phe Ser Ala Pro Glu Leu Ile Thr Asp Glu Ile Val Glu

100 105 110

Met Ile Arg Asp Leu Ile Pro Leu Ala Pro Leu His Asn Pro Ala Asn

115 120 125

Val Asp Gly Ile Asp Val Ala Arg Lys Ile Leu Pro Asp Val Pro His

130 135 140

Val Ala Val Phe Asp Thr Gly Phe Phe His Ser Leu Pro Pro Ala Ala

145 150 155 160

Ala Leu Tyr Ala Ile Asn Lys Asp Val Ala Ala Glu His Gly Ile Arg

165 170 175

Arg Tyr Gly Phe His Gly Thr Ser His Glu Phe Val Ser Lys Arg Val

180 185 190

Val Glu Ile Leu Glu Lys Pro Thr Glu Asp Ile Asn Thr Ile Thr Phe

195 200 205

His Leu Gly Asn Gly Ala Ser Met Ala Ala Val Gln Gly Gly Arg Ala

210 215 220

Val Asp Thr Ser Met Gly Met Thr Pro Leu Ala Gly Leu Val Met Gly

225 230 235 240

Thr Arg Ser Gly Asp Ile Asp Pro Gly Ile Val Phe His Leu Ser Arg

245 250 255

Thr Ala Gly Met Ser Ile Asp Glu Ile Asp Asn Leu Leu Asn Lys Lys

260 265 270

Ser Gly Val Lys Gly Leu Ser Gly Val Asn Asp Phe Arg Glu Leu Arg

275 280 285

Glu Met Ile Asp Asn Asn Asp Gln Asp Ala Trp Ser Ala Tyr Asn Ile

290 295 300

Tyr Ile His Gln Leu Arg Arg Tyr Leu Gly Ser Tyr Met Val Ala Leu

305 310 315 320

Gly Arg Val Asp Thr Ile Val Phe Thr Ala Gly Val Gly Glu Asn Ala

325 330 335

Gln Phe Val Arg Glu Asp Ala Leu Ala Gly Leu Glu Met Tyr Gly Ile

340 345 350

Glu Ile Asp Pro Glu Arg Asn Ala Leu Pro Asn Asp Gly Pro Arg Leu

355 360 365

Ile Ser Thr Asp Ala Ser Lys Val Lys Val Phe Val Ile Pro Thr Asn

370 375 380

Glu Glu Leu Ala Ile Ala Arg Tyr Ala Val Lys Phe Ala

385 390 395

<210>45

<211>2056

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2026)

<223>RXA01478

<400>45

tggcagagca ttcgactgtt aatcgaaggg tcactggttc gagcccagtt ggaggagcaa 60

tacacacagc ccgccgtttt tcttaaaacg gcgggttttg ttg tgg agg ggc gcg 115

Leu Trp Arg Gly Ala

1 5

tcg aaa agc aat ttt ttt tcc aag ata gct cac ttt att gga gtc acc 163

Ser Lys Ser Asn Phe Phe Ser Lys Ile Ala His Phe Ile Gly Val Thr

10 15 20

tgg cct gaa atc ctc tac tct ggg cgc atg acc att cca gga gct tcc 211

Trp Pro Glu Ile Leu Tyr Ser Gly Arg Met Thr Ile Pro Gly Ala Ser

25 30 35

aca cag act gat atc cct ctg gac aca ctt ctt gag gat tac gcg cta 259

Thr Gln Thr Asp Ile Pro Leu Asp Thr Leu Leu Glu Asp Tyr Ala Leu

40 45 50

ctg tca gac act cac acc ggc gct ctg ctg tcc aac atg ggc agt ttg 307

Leu Ser Asp Thr His Thr Gly Ala Leu Leu Ser Asn Met Gly Ser Leu

55 60 65

gac tgg ttg tgc ctg cct cgt ttt gat tcc caa gcc atg ttc acc agg 355

Asp Trp Leu Cys Leu Pro Arg Phe Asp Ser Gln Ala Met Phe Thr Arg

70 75 80 85

ctg ctt ggt gat cgc gag cac gga cac tgg agt ttg cgt gtc cca ggt 403

Leu Leu Gly Asp Arg Glu His Gly His Trp Ser Leu Arg Val Pro Gly

90 95 100

ggt gag gtg atc agc caa aac tac ctc ggc gat tcc ttc gtg gtg cag 451

Gly Glu Val Ile Ser Gln Asn Tyr Leu Gly Asp Ser Phe Val Val Gln

105 110 115

acc gtg tgg cgt tca gag acc ggt act gcc cgg gtt gtt gat ttc atg 499

Thr Val Trp Arg Ser Glu Thr Gly Thr Ala Arg Val Val Asp Phe Met

120 125 130

cca att cac ggt caa gaa caa ccc gat atc acc gac ctg gtg cgc tct 547

Pro Ile His Gly Gln Glu Gln Pro Asp Ile Thr Asp Leu Val Arg Ser

135 140 145

gtg cac tgc gtg gaa ggc gaa gtg gat gtg gaa tcg atc ctg cgc ctg 595

Val His Cys Val Glu Gly Glu Val Asp Val Glu Ser Ile Leu Arg Leu

150 155 160 165

cgt ttt gat tat ggc gag tcc act ccg tat ttc cgc acc agc act gtc 643

Arg Phe Asp Tyr Gly Glu Ser Thr Pro Tyr Phe Arg Thr Ser Thr Val

170 175 180

gac ggc atc agc atc gtg cag gct gtc gcc ggc ccc aat gcg gta tat 691

Asp Gly Ile Ser Ile Val Gln Ala Val Ala Gly Pro Asn Ala Val Tyr

185 190 195

gtt cgt gga cct gag atg cca cac cgc cct gca aag gat tgt cac agt 739

Val Arg Gly Pro Glu Met Pro His Arg Pro Ala Lys Asp Cys His Ser

200 205 210

ggc acc ttc cac ctg acg gcc ggc gaa tcc gtg gaa tgg gtt ctc acc 787

Gly Thr Phe His Leu Thr Ala Gly Glu Ser Val Glu Trp Val Leu Thr

215 220 225

tgg gca ccg tcg ttc gaa ccg cat ccc ccc atg ccg gat tac acc cgc 835

Trp Ala Pro Ser Phe Glu Pro His Pro Pro Met Pro Asp Tyr Thr Arg

230 235 240 245

tct ttg gag agc acc ttg agc ttc tgg gca tca tgg gtt gaa gag ctc 883

Ser Leu Glu Ser Thr Leu Ser Phe Trp Ala Ser Trp Val Glu Glu Leu

250 255 260

ccc cac cag cgc ctc tac gac gct gaa gtc cgc cgc tcc atg ctc gta 931

Pro His Gln Arg Leu Tyr Asp Ala Glu Val Arg Arg Ser Met Leu Val

265 270 275

ctg cgc gcc ttg acc gat cta caa acc ggt ggc atc gtg gcc gca ccg 979

Leu Arg Ala Leu Thr Asp Leu Gln Thr Gly Gly Ile Val Ala Ala Pro

280 285 290

acc acc tca cta cca gag gat ttc gga ggc atc cgt aac tgg gac tac 1027

Thr Thr Ser Leu Pro Glu Asp Phe Gly Gly Ile Arg Asn Trp Asp Tyr

295 300 305

cgc tac gtg tgg ctg cgc gac tcc gca ctc acc att gaa gcc ctc gtg 1075

Arg Tyr Val Trp Leu Arg Asp Ser Ala Leu Thr Ile Glu Ala Leu Val

310 315 320 325

gaa tac gga ttc tcc caa gca gcc ctc caa tgg cgc acc tgg ctg ctg 1123

Glu Tyr Gly Phe Ser Gln Ala Ala Leu Gln Trp Arg Thr Trp Leu Leu

330 335 340

cgc gcc atc gca ggc gac ccg gaa aac ctc cgc atc atg tat ggc ctc 1171

Arg Ala Ile Ala Gly Asp Pro Glu Asn Leu Arg Ile Met Tyr Gly Leu

345 350 355

ggc ggc gaa cga cac ctc cct gaa cgc gaa ctc caa cac ctg cgc gga 1219

Gly Gly Glu Arg His Leu Pro Glu Arg Glu Leu Gln His Leu Arg Gly

360 365 370

tac gaa aac tcc gtg cct gtt cgc gtt ggc aat gga gcc gcc gaa caa 1267

Tyr Glu Asn Ser Val Pro Val Arg Val Gly Asn Gly Ala Ala Glu Gln

375 380 385

tac caa gca gat gtc gtc ggc gaa gta atg gtc gcg ctt gaa acc atc 1315

Tyr Gln Ala Asp Val Val Gly Glu Val Met Val Ala Leu Glu Thr Ile

390 395 400 405

cgc cgc gcc ggg tgc ctc gag gac gaa ttc tcc tgg ggc atg caa aaa 1363

Arg Arg Ala Gly Cys Leu Glu Asp Glu Phe Ser Trp Gly Met Gln Lys

410 415 420

gcc atc ctc gat ttc caa gaa gcc aac ttc gac cgc aag gat caa ggc 1411

Ala Ile Leu Asp Phe Gln Glu Ala Asn Phe Asp Arg Lys Asp Gln Gly

425 430 435

atc tgg gaa atg cgc tcc gaa ccg caa tat ttc acc cac ggc cgc gcc 1459

Ile Trp Glu Met Arg Ser Glu Pro Gln Tyr Phe Thr His Gly Arg Ala

440 445 450

atg atg tgg gcc ggc ttc gac cgc ggc atc aaa gcc atc gaa gaa ttc 1507

Met Met Trp Ala Gly Phe Asp Arg Gly Ile Lys Ala Ile Glu Glu Phe

455 460 465

aac ctc gac ggc ccc atc gag cgc tgg cgt gaa ctc cgc gcc aaa ctc 1555

Asn Leu Asp Gly Pro Ile Glu Arg Trp Arg Glu Leu Arg Ala Lys Leu

470 475 480 485

cgc gaa gaa atc atg acc aac ggc ttc aac gaa gag atc caa tcc ttc 1603

Arg Glu Glu Ile Met Thr Asn Gly Phe Asn Glu Glu Ile Gln Ser Phe

490 495 500

acc cag tgc tac gac aac acc caa gtc gac gcc tcg ctg ctt cag ctc 1651

Thr Gln Cys Tyr Asp Asn Thr Gln Val Asp Ala Ser Leu Leu Gln Leu

505 510 515

gcc caa ata ggc ttc atc ggc ttc gac gat cca aaa atg ctc agc acc 1699

Ala Gln Ile Gly Phe Ile Gly Phe Asp Asp Pro Lys Met Leu Ser Thr

520 525 530

gta gcg cgc att gag caa gag ctt ctc gac gcc cac ggc ttt ctt cac 1747

Val Ala Arg Ile Glu Gln Glu Leu Leu Asp Ala His Gly Phe Leu His

535 540 545

agg tac cac acc gac ggg tct gac ggc ctt gcc ggc gac gaa tac ccc 1795

Arg Tyr His Thr Asp Gly Ser Asp Gly Leu Ala Gly Asp Glu Tyr Pro

550 555 560 565

ttc ctc atc tgt tca ttc tgg ctg gta gaa caa tac gca agc tcc aac 1843

Phe Leu Ile Cys Ser Phe Trp Leu Val Glu Gln Tyr Ala Ser Ser Asn

570 575 580

cgc ctc gac gag gcc aaa gaa aag atg aac cgc atc ctt gcc gtc caa 1891

Arg Leu Asp Glu Ala Lys Glu Lys Met Asn Arg Ile Leu Ala Val Gln

585 590 595

agc cca ctt ggc cta ctg gct gag gaa tac tcc acc cac cat ggc agg 1939

Ser Pro Leu Gly Leu Leu Ala Glu Glu Tyr Ser Thr His His Gly Arg

600 605 610

ctc gct gga aac tac cct cag gcc ttt tcc cac att ggt ctg atc agc 1987

Leu Ala Gly Asn Tyr Pro Gln Ala Phe Ser His Ile Gly Leu Ile Ser

615 620 625

gct gcc cgt gcc ata aat ttc gaa gaa gcg cga aac agg tagagtctaa 2036

Ala Ala Arg Ala Ile Asn Phe Glu Glu Ala Arg Asn Arg

630 635 640

ggtgtcattc ttggtacaag 2056

<210>46

<211>642

<212>PRT

<213>谷氨酸棒状杆菌

<400>46

Leu Trp Arg Gly Ala Ser Lys Ser Asn Phe Phe Ser Lys Ile Ala His

1 5 10 15

Phe Ile Gly Val Thr Trp Pro Glu Ile Leu Tyr Ser Gly Arg Met Thr

20 25 30

Ile Pro Gly Ala Ser Thr Gln Thr Asp Ile Pro Leu Asp Thr Leu Leu

35 40 45

Glu Asp Tyr Ala Leu Leu Ser Asp Thr His Thr Gly Ala Leu Leu Ser

50 55 60

Asn Met Gly Ser Leu Asp Trp Leu Cys Leu Pro Arg Phe Asp Ser Gln

65 70 75 80

Ala Met Phe Thr Arg Leu Leu Gly Asp Arg Glu His Gly His Trp Ser

85 90 95

Leu Arg Val Pro Gly Gly Glu Val Ile Ser Gln Asn Tyr Leu Gly Asp

100 105 110

Ser Phe Val Val Gln Thr Val Trp Arg Ser Glu Thr Gly Thr Ala Arg

115 120 125

Val Val Asp Phe Met Pro Ile His Gly Gln Glu Gln Pro Asp Ile Thr

130 135 140

Asp Leu Val Arg Ser Val His Cys Val Glu Gly Glu Val Asp Val Glu

145 150 155 160

Ser Ile Leu Arg Leu Arg Phe Asp Tyr Gly Glu Ser Thr Pro Tyr Phe

165 170 175

Arg Thr Ser Thr Val Asp Gly Ile Ser Ile Val Gln Ala Val Ala Gly

180 185 190

Pro Asn Ala Val Tyr Val Arg Gly Pro Glu Met Pro His Arg Pro Ala

195 200 205

Lys Asp Cys His Ser Gly Thr Phe His Leu Thr Ala Gly Glu Ser Val

210 215 220

Glu Trp Val Leu Thr Trp Ala Pro Ser Phe Glu Pro His Pro Pro Met

225 230 235 240

Pro Asp Tyr Thr Arg Ser Leu Glu Ser Thr Leu Ser Phe Trp Ala Ser

245 250 255

Trp Val Glu Glu Leu Pro His Gln Arg Leu Tyr Asp Ala Glu Val Arg

260 265 270

Arg Ser Met Leu Val Leu Arg Ala Leu Thr Asp Leu Gln Thr Gly Gly

275 280 285

Ile Val Ala Ala Pro Thr Thr Ser Leu Pro Glu Asp Phe Gly Gly Ile

290 295 300

Arg Asn Trp Asp Tyr Arg Tyr Val Trp Leu Arg Asp Ser Ala Leu Thr

305 310 315 320

Ile Glu Ala Leu Val Glu Tyr Gly Phe Ser Gln Ala Ala Leu Gln Trp

325 330 335

Arg Thr Trp Leu Leu Arg Ala Ile Ala Gly Asp Pro Glu Asn Leu Arg

340 345 350

Ile Met Tyr Gly Leu Gly Gly Glu Arg His Leu Pro Glu Arg Glu Leu

355 360 365

Gln His Leu Arg Gly Tyr Glu Asn Ser Val Pro Val Arg Val Gly Asn

370 375 380

Gly Ala Ala Glu Gln Tyr Gln Ala Asp Val Val Gly Glu Val Met Val

385 390 395 400

Ala Leu Glu Thr Ile Arg Arg Ala Gly Cys Leu Glu Asp Glu Phe Ser

405 410 415

Trp Gly Met Gln Lys Ala Ile Leu Asp Phe Gln Glu Ala Asn Phe Asp

420 425 430

Arg Lys Asp Gln Gly Ile Trp Glu Met Arg Ser Glu Pro Gln Tyr Phe

435 440 445

Thr His Gly Arg Ala Met Met Trp Ala Gly Phe Asp Arg Gly Ile Lys

450 455 460

Ala Ile Glu Glu Phe Asn Leu Asp Gly Pro Ile Glu Arg Trp Arg Glu

465 470 475 480

Leu Arg Ala Lys Leu Arg Glu Glu Ile Met Thr Asn Gly Phe Asn Glu

485 490 495

Glu Ile Gln Ser Phe Thr Gln Cys Tyr Asp Asn Thr Gln Val Asp Ala

500 505 510

Ser Leu Leu Gln Leu Ala Gln Ile Gly Phe Ile Gly Phe Asp Asp Pro

515 520 525

Lys Met Leu Ser Thr Val Ala Arg Ile Glu Gln Glu Leu Leu Asp Ala

530 535 540

His Gly Phe Leu His Arg Tyr His Thr Asp Gly Ser Asp Gly Leu Ala

545 550 555 560

Gly Asp Glu Tyr Pro Phe Leu Ile Cys Ser Phe Trp Leu Val Glu Gln

565 570 575

Tyr Ala Ser Ser Asn Arg Leu Asp Glu Ala Lys Glu Lys Met Asn Arg

580 585 590

Ile Leu Ala Val Gln Ser Pro Leu Gly Leu Leu Ala Glu Glu Tyr Ser

595 600 605

Thr His His Gly Arg Leu Ala Gly Asn Tyr Pro Gln Ala Phe Ser His

610 615 620

Ile Gly Leu Ile Ser Ala Ala Arg Ala Ile Asn Phe Glu Glu Ala Arg

625 630 635 640

Asn Arg

<210>47

<211>2272

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2242)

<223>RXA01554

<400>47

tggcttaaac cttatttgta gttgtcaata aatatgaaat tccttagcaa cttgtttaaa 60

tagacgtata aacaagtttg aaaaaggaag gttatccatc ttg aaa aag cat gtg 115

Leu Lys Lys His Val

1 5

acc tca gcc gtt acc gcc gtg gtg acg gct ttt tca acg gct gcg ctt 163

Thr Ser Ala Val Thr Ala Val Val Thr Ala Phe Ser Thr Ala Ala Leu

10 15 20

ggt tta agt att gcc gtt tct cct gct gtt gcc caa gtg gct aat cca 211

Gly Leu Ser Ile Ala Val Ser Pro Ala Val Ala Gln Val Ala Asn Pro

25 30 35

gct cca gat ctt tct gcg ccg tat aca tgg gtg gaa gag ttt gat tcc 259

Ala Pro Asp Leu Ser Ala Pro Tyr Thr Trp Val Glu Glu Phe Asp Ser

40 45 50

gag gat gct ctc aaa ggg tgg aac att ttc cgc cag cca gat tat ggc 307

Glu Asp Ala Leu Lys Gly Trp Asn Ile Phe Arg Gln Pro Asp Tyr Gly

55 60 65

agc gac aaa gtt ctc tat acc gaa gat gct tta agt atc gaa gat ggc 355

Ser Asp Lys Val Leu Tyr Thr Glu Asp Ala Leu Ser Ile Glu Asp Gly

70 75 80 85

aag ctc acc atc acc act cag cgc cac tgc gtt gac gaa gac ttc gcg 403

Lys Leu Thr Ile Thr Thr Gln Arg His Cys Val Asp Glu Asp Phe Ala

90 95 100

atc agt gat cct gtc aac cgc gga aag ctc aat gac agc acc gcg caa 451

Ile Ser Asp Pro Val Asn Arg Gly Lys Leu Asn Asp Ser Thr Ala Gln

105 110 115

gtt gaa cct tgt gct cca ggt cag ttt gaa aag ttc acc agt gcg cgc 499

Val Glu Pro Cys Ala Pro Gly Gln Phe Glu Lys Phe Thr Ser Ala Arg

120 125 130

atc gtc act ccg aaa att gct cgt gga gag ttc gac ctt tct gtc act 547

Ile Val Thr Pro Lys Ile Ala Arg Gly Glu Phe Asp Leu Ser Val Thr

135 140 145

gca act ctt aac acc ggt ggc gtc gaa ggt gtc cga agt gcc att tgg 595

Ala Thr Leu Asn Thr Gly Gly Val Glu Gly Val Arg Ser Ala Ile Trp

150 155 160 165

atg caa aac ggt gaa cag gcg tgt tcc tca gca acc aac aat ggc ctc 643

Met Gln Asn Gly Glu Gln Ala Cys Ser Ser Ala Thr Asn Asn Gly Leu

170 175 180

tac gga gaa cta gac ctg gta gag cac ttt tct tac gat ctt cgc tcg 691

Tyr Gly Glu Leu Asp Leu Val Glu His Phe Ser Tyr Asp Leu Arg Ser

185 190 195

cca tgg tct cca tca aac acc cac ttg ggt tgt gat cct gaa agt gtc 739

Pro Trp Ser Pro Ser Asn Thr His Leu Gly Cys Asp Pro Glu Ser Val

200 205 210

aac ggc acc aac cgt gca cct cgt gaa ctt aaa cta gat gag tca ctc 787

Asn Gly Thr Asn Arg Ala Pro Arg Glu Leu Lys Leu Asp Glu Ser Leu

215 220 225

gat ggc gtg gag cac acc tgg act gtg agc acc acc cgc gac ggc gtt 835

Asp Gly Val Glu His Thr Trp Thr Val Ser Thr Thr Arg Asp Gly Val

230 235 240 245

gag tac ttc att gat gat gag gcg att aac cgc cag tca tgg cgc aac 883

Glu Tyr Phe Ile Asp Asp Glu Ala Ile Asn Arg Gln Ser Trp Arg Asn

250 255 260

gat gtc act ttg ggg cat gcc gaa att gat gat ttc ggg atc tcc gcg 931

Asp Val Thr Leu Gly His Ala Glu Ile Asp Asp Phe Gly Ile Ser Ala

265 270 275

cag acg ttt gat gag atc gtc gac cgc gaa tgg act ctc act ctt aat 979

Gln Thr Phe Asp Glu Ile Val Asp Arg Glu Trp Thr Leu Thr Leu Asn

280 285 290

caa aag gta gaa agc gcc gac tgg gca aaa cca cgt tcc tct gag gaa 1027

Gln Lys Val Glu Ser Ala Asp Trp Ala Lys Pro Arg Ser Ser Glu Glu

295 300 305

gat ttc cca gtc cgg tcc atg gtg att gac cgc atc gag gtc acc gga 1075

Asp Phe Pro Val Arg Ser Met Val Ile Asp Arg Ile Glu Val Thr Gly

310 315 320 325

tct ccc gca gta tct gaa gac acc ccc atg cca gat acc acc cag ctt 1123

Ser Pro Ala Val Ser Glu Asp Thr Pro Met Pro Asp Thr Thr Gln Leu

330 335 340

ttg acc caa gac act ctg gaa tac ctc ggt cgc atg cca gtg ctg gaa 1171

Leu Thr Gln Asp Thr Leu Glu Tyr Leu Gly Arg Met Pro Val Leu Glu

345 350 355

cgc tac gag cca gca agt gct gat ttt gcc gat ggc cgc agg cct tcc 1219

Arg Tyr Glu Pro Ala Ser Ala Asp Phe Ala Asp Gly Arg Arg Pro Ser

360 365 370

tgg aac tac ttc aat ttg aag gaa tcg tgg cag aat cca gaa ctc gag 1267

Trp Asn Tyr Phe Asn Leu Lys Glu Ser Trp Gln Asn Pro Glu Leu Glu

375 380 385

caa cgc cca gaa gct gtc gaa ttc gtt gat gga cgc atg gat atc gtg 1315

Gln Arg Pro Glu Ala Val Glu Phe Val Asp Gly Arg Met Asp Ile Val

390 395 400 405

acc cgt cgc cac tgt ctg gcc acc act gat gac atc gcc act ccg gaa 1363

Thr Arg Arg His Cys Leu Ala Thr Thr Asp Asp Ile Ala Thr Pro Glu

410 415 420

aac gca cag gag gaa ccg tgc gcg ccg ggt gag gtg aca cgc tac agc 1411

Asn Ala Gln Glu Glu Pro Cys Ala Pro Gly Glu Val Thr Arg Tyr Ser

425 430 435

tca gcg cgt gtc cac ctt cca gag atc ccc gcc ggc aac ttc cgg ctc 1459

Ser Ala Arg Val His Leu Pro Glu Ile Pro Ala Gly Asn Phe Arg Leu

440 445 450

acc gtg cgc gca cgg gcg cag tcc gaa gag ctt gtc gac ggc gtc cgc 1507

Thr Val Arg Ala Arg Ala Gln Ser Glu Glu Leu Val Asp Gly Val Arg

455 460 465

ccc gct atc tgg atg cag aac aat acc aac ttc tgt gct gac aac gat 1555

Pro Ala Ile Trp Met Gln Asn Asn Thr Asn Phe Cys Ala Asp Asn Asp

470 475 480 485

gga cgc cct tat ggt gaa ctg gat att act gag ttc tac agc tct cgt 1603

Gly Arg Pro Tyr Gly Glu Leu Asp Ile Thr Glu Phe Tyr Ser Ser Arg

490 495 500

gtg aac acc cag tac tcg gca gta cac ctt gga tgt gct ggc aac cgc 1651

Val Asn Thr Gln Tyr Ser Ala Val His Leu Gly Cys Ala Gly Asn Arg

505 510 515

cca gag atg aag ctt cgc caa atg gaa atg gaa gag tcc atg ttt ggg 1699

Pro Glu Met Lys Leu Arg Gln Met Glu Met Glu Glu Ser Met Phe Gly

520 525 530

gat tgg cat gac tgg ggc gtc gaa gtc ttc gac ggc cag atc gta ttc 1747

Asp Trp His Asp Trp Gly Val Glu Val Phe Asp Gly Gln Ile Val Phe

535 540 545

acc att gac ggc aag gca gta act tcc tct ggc aaa gat gtc ttt ggc 1795

Thr Ile Asp Gly Lys Ala Val Thr Ser Ser Gly Lys Asp Val Phe Gly

550 555 560 565

aac tct gtt acc cca gcc gct gca cct ctt cgc ccc gcg cac ttc aag 1843

Asn Ser Val Thr Pro Ala Ala Ala Pro Leu Arg Pro Ala His Phe Lys

570 575 580

ttg tcg gaa gag gaa tac cgt gaa gtc atc ggg cag cct tgg cac ctt 1891

Leu Ser Glu Glu Glu Tyr Arg Glu Val Ile Gly Gln Pro Trp His Leu

585 590 595

att ttg aac acc atg gtg gag cag tct ggc aaa gac agc tgg att aca 1939

Ile Leu Asn Thr Met Val Glu Gln Ser Gly Lys Asp Ser Trp Ile Thr

600 605 610

gcg gtc gac aat aac gag gcg ttc cca gaa cac cgc ttc caa att gac 1987

Ala Val Asp Asn Asn Glu Ala Phe Pro Glu His Arg Phe Gln Ile Asp

615 620 625

cat gtg gca gta gat atc gag tct gac tct gtg gac aat gta tgg cct 2035

His Val Ala Val Asp Ile Glu Ser Asp Ser Val Asp Asn Val Trp Pro

630 635 640 645

gac gct gcg aat gaa atc cca gac aat gtt ggt att gaa gac tct gat 2083

Asp Ala Ala Asn Glu Ile Pro Asp Asn Val Gly Ile Glu Asp Ser Asp

650 655 660

gat ggc agc gac ctg gag gtt ggt tcg acc gga agc tct aca gct gag 2131

Asp Gly Ser Asp Leu Glu Val Gly Ser Thr Gly Ser Ser Thr Ala Glu

665 670 675

acc gtg agc tgg atc tcg ttg ttc acc gcg ttg agc tcg ctg gtc ttc 2179

Thr Val Ser Trp Ile Ser Leu Phe Thr Ala Leu Ser Ser Leu Val Phe

680 685 690

aca ctg gct ctc aat caa gaa gca ttg cag aat ttg att aat cag ttc 2227

Thr Leu Ala Leu Asn Gln Glu Ala Leu Gln Asn Leu Ile Asn Gln Phe

695 700 705

atg aga cag ttc aag taatccttgt gggcgctttg tctacttcac 2272

Met Arg Gln Phe Lys

710

<210>48

<211>714

<212>PRT

<213>谷氨酸棒状杆菌

<400>48

Leu Lys Lys His Val Thr Ser Ala Val Thr Ala Val Val Thr Ala Phe

1 5 10 15

Ser Thr Ala Ala Leu Gly Leu Ser Ile Ala Val Ser Pro Ala Val Ala

20 25 30

Gln Val Ala Asn Pro Ala Pro Asp Leu Ser Ala Pro Tyr Thr Trp Val

35 40 45

Glu Glu Phe Asp Ser Glu Asp Ala Leu Lys Gly Trp Asn Ile Phe Arg

50 55 60

Gln Pro Asp Tyr Gly Ser Asp Lys Val Leu Tyr Thr Glu Asp Ala Leu

65 70 75 80

Ser Ile Glu Asp Gly Lys Leu Thr Ile Thr Thr Gln Arg His Cys Val

85 90 95

Asp Glu Asp Phe Ala Ile Ser Asp Pro Val Asn Arg Gly Lys Leu Asn

100 105 110

Asp Ser Thr Ala Gln Val Glu Pro Cys Ala Pro Gly Gln Phe Glu Lys

115 120 125

Phe Thr Ser Ala Arg Ile Val Thr Pro Lys Ile Ala Arg Gly Glu Phe

130 135 140

Asp Leu Ser Val Thr Ala Thr Leu Asn Thr Gly Gly Val Glu Gly Val

145 150 155 160

Arg Ser Ala Ile Trp Met Gln Asn Gly Glu Gln Ala Cys Ser Ser Ala

165 170 175

Thr Asn Asn Gly Leu Tyr Gly Glu Leu Asp Leu Val Glu His Phe Ser

180 185 190

Tyr Asp Leu Arg Ser Pro Trp Ser Pro Ser Asn Thr His Leu Gly Cys

195 200 205

Asp Pro Glu Ser Val Asn Gly Thr Asn Arg Ala Pro Arg Glu Leu Lys

210 215 220

Leu Asp Glu Ser Leu Asp Gly Val Glu His Thr Trp Thr Val Ser Thr

225 230 235 240

Thr Arg Asp Gly Val Glu Tyr Phe Ile Asp Asp Glu Ala Ile Asn Arg

245 250 255

Gln Ser Trp Arg Asn Asp Val Thr Leu Gly His Ala Glu Ile Asp Asp

260 265 270

Phe Gly Ile Ser Ala Gln Thr Phe Asp Glu Ile Val Asp Arg Glu Trp

275 280 285

Thr Leu Thr Leu Asn Gln Lys Val Glu Ser Ala Asp Trp Ala Lys Pro

290 295 300

Arg Ser Ser Glu Glu Asp Phe Pro Val Arg Ser Met Val Ile Asp Arg

305 310 315 320

Ile Glu Val Thr Gly Ser Pro Ala Val Ser Glu Asp Thr Pro Met Pro

325 330 335

Asp Thr Thr Gln Leu Leu Thr Gln Asp Thr Leu Glu Tyr Leu Gly Arg

340 345 350

Met Pro Val Leu Glu Arg Tyr Glu Pro Ala Ser Ala Asp Phe Ala Asp

355 360 365

Gly Arg Arg Pro Ser Trp Asn Tyr Phe Asn Leu Lys Glu Ser Trp Gln

370 375 380

Asn Pro Glu Leu Glu Gln Arg Pro Glu Ala Val Glu Phe Val Asp Gly

385 390 395 400

Arg Met Asp Ile Val Thr Arg Arg His Cys Leu Ala Thr Thr Asp Asp

405 410 415

Ile Ala Thr Pro Glu Asn Ala Gln Glu Glu Pro Cys Ala Pro Gly Glu

420 425 430

Val Thr Arg Tyr Ser Ser Ala Arg Val His Leu Pro Glu Ile Pro Ala

435 440 445

Gly Asn Phe Arg Leu Thr Val Arg Ala Arg Ala Gln Ser Glu Glu Leu

450 455 460

Val Asp Gly Val Arg Pro Ala Ile Trp Met Gln Asn Asn Thr Asn Phe

465 470 475 480

Cys Ala Asp Asn Asp Gly Arg Pro Tyr Gly Glu Leu Asp Ile Thr Glu

485 490 495

Phe Tyr Ser Ser Arg Val Asn Thr Gln Tyr Ser Ala Val His Leu Gly

500 505 510

Cys Ala Gly Asn Arg Pro Glu Met Lys Leu Arg Gln Met Glu Met Glu

515 520 525

Glu Ser Met Phe Gly Asp Trp His Asp Trp Gly Val Glu Val Phe Asp

530 535 540

Gly Gln Ile Val Phe Thr Ile Asp Gly Lys Ala Val Thr Ser Ser Gly

545 550 555 560

Lys Asp Val Phe Gly Asn Ser Val Thr Pro Ala Ala Ala Pro Leu Arg

565 570 575

Pro Ala His Phe Lys Leu Ser Glu Glu Glu Tyr Arg Glu Val Ile Gly

580 585 590

Gln Pro Trp His Leu Ile Leu Asn Thr Met Val Glu Gln Ser Gly Lys

595 600 605

Asp Ser Trp Ile Thr Ala Val Asp Asn Asn Glu Ala Phe Pro Glu His

610 615 620

Arg Phe Gln Ile Asp His Val Ala Val Asp Ile Glu Ser Asp Ser Val

625 630 635 640

Asp Asn Val Trp Pro Asp Ala Ala Asn Glu Ile Pro Asp Asn Val Gly

645 650 655

Ile Glu Asp Ser Asp Asp Gly Ser Asp Leu Glu Val Gly Ser Thr Gly

660 665 670

Ser Ser Thr Ala Glu Thr Val Ser Trp Ile Ser Leu Phe Thr Ala Leu

675 680 685

Ser Ser Leu Val Phe Thr Leu Ala Leu Asn Gln Glu Ala Leu Gln Asn

690 695 700

Leu Ile Asn Gln Phe Met Arg Gln Phe Lys

705 710

<210>49

<211>2038

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2008)

<223>RXA01562

<400>49

gcaggtgcac atttgttttg tcacctgcac aaaagtgtcg ccagcccgat acttgtacaa 60

ccgtccgcat ccgagaagca aaggtgtctg actcgcgcca atg gga att ctg aac 115

Met Gly Ile Leu Asn

1 5

agt att tca aca cct gct gac tta aag gcc ctt aat gat gag gat ttg 163

Ser Ile Ser Thr Pro Ala Asp Leu Lys Ala Leu Asn Asp Glu Asp Leu

10 15 20

gac gct ctt gcc aaa gaa atc cga act ttc ctg gtc gat aaa gtc gca 211

Asp Ala Leu Ala Lys Glu Ile Arg Thr Phe Leu Val Asp Lys Val Ala

25 30 35

gca act ggt ggc cac tta ggt cca aat ttg ggc gta gtg gaa tta acc 259

Ala Thr Gly Gly His Leu Gly Pro Asn Leu Gly Val Val Glu Leu Thr

40 45 50

atc ggt ctt cat cga gtt ttc gat tcg cct caa gac ccg atc atc ttt 307

Ile Gly Leu His Arg Val Phe Asp Ser Pro Gln Asp Pro Ile Ile Phe

55 60 65

gat act tct cac cag tcc tat gtg cat aag atc ctg acg ggt cgc gct 355

Asp Thr Ser His Gln Ser Tyr Val His Lys Ile Leu Thr Gly Arg Ala

70 75 80 85

aaa gat ttt gat tct ttg cgt caa aaa gat ggc ctt tct ggt tac acc 403

Lys Asp Phe Asp Ser Leu Arg Gln Lys Asp Gly Leu Ser Gly Tyr Thr

90 95 100

tgc cgt gct gaa agt gag cac gat tgg act gag tct tcg cat gct tcg 451

Cys Arg Ala Glu Ser Glu His Asp Trp Thr Glu Ser Ser His Ala Ser

105 110 115

gcg gcc ttg tct tat gcg gat ggt ttg tct aaa gcc aag cag ttg gat 499

Ala Ala Leu Ser Tyr Ala Asp Gly Leu Ser Lys Ala Lys Gln Leu Asp

120 125 130

ggc gat acc acg cat agt gtg gtt gct gtc gtt ggt gat ggc gct cta 547

Gly Asp Thr Thr His Ser Val Val Ala Val Val Gly Asp Gly Ala Leu

135 140 145

act ggc ggc atg tgt tgg gaa gca ctg aac aat att gct gct ggt aaa 595

Thr Gly Gly Met Cys Trp Glu Ala Leu Asn Asn Ile Ala Ala Gly Lys

150 155 160 165

gac cgc aaa gtt gtt gtc gta gtc aat gac aat ggc cgg agt tat tct 643

Asp Arg Lys Val Val Val Val Val Asn Asp Asn Gly Arg Ser Tyr Ser

170 175 180

cca acc att ggc gga ttt gcg gaa aac ctt gcg ggc ctt cgc atg cag 691

Pro Thr Ile Gly Gly Phe Ala Glu Asn Leu Ala Gly Leu Arg Met Gln

185 190 195

cct ttc tat gat cgc ttc atg gaa aag ggc aag acg tcc ctg aaa tcc 739

Pro Phe Tyr Asp Arg Phe Met Glu Lys Gly Lys Thr Ser Leu Lys Ser

200 205 210

atg ggg tgg gta ggg gag cgt act ttt gaa gcg ctc cat gca ttt aaa 787

Met Gly Trp Val Gly Glu Arg Thr Phe Glu Ala Leu His Ala Phe Lys

215 220 225

gaa ggt gtg aag agc acc gtc att ccc acc gaa atg ttc cct gaa ctg 835

Glu Gly Val Lys Ser Thr Val Ile Pro Thr Glu Met Phe Pro Glu Leu

230 235 240 245

ggc atg aaa tac gtg ggt ccg gtt gat gga cat aac caa aaa gct gtc 883

Gly Met Lys Tyr Val Gly Pro Val Asp Gly His Asn Gln Lys Ala Val

250 255 260

gac aat gcg ctg aaa tac gct cat gat tat gat ggc ccc atc atc gtg 931

Asp Asn Ala Leu Lys Tyr Ala His Asp Tyr Asp Gly Pro Ile Ile Val

265 270 275

cac atg gtc acc gaa aag ggt cgt ggt tac gcg cct gct gag cag gat 979

His Met Val Thr Glu Lys Gly Arg Gly Tyr Ala Pro Ala Glu Gln Asp

280 285 290

ttg gac gaa ttg atg cac tcc acg ggc gtc atc gat ccg ctc aca gga 1027

Leu Asp Glu Leu Met His Ser Thr Gly Val Ile Asp Pro Leu Thr Gly

295 300 305

gct cct aaa tct gca tca aag ccc ggt tgg acc tct gtg ttc agc gat 1075

Ala Pro Lys Ser Ala Ser Lys Pro Gly Trp Thr Ser Val Phe Ser Asp

310 315 320 325

gag ctg gtc aag att ggt gcg cag aat gaa aac gtt gtt gcc atc acc 1123

Glu Leu Val Lys Ile Gly Ala Gln Asn Glu Asn Val Val Ala Ile Thr

330 335 340

gcc gcg atg gca ggt cct acc ggt ctg tcc aag ttc gaa gcc aat ttc 1171

Ala Ala Met Ala Gly Pro Thr Gly Leu Ser Lys Phe Glu Ala Asn Phe

345 350 355

ccc aac cga ttc ttt gat gtc ggc att gct gag cag cac gcg gta act 1219

Pro Asn Arg Phe Phe Asp Val Gly Ile Ala Glu Gln His Ala Val Thr

360 365 370

tct gcc gca ggc ctc gca ttg ggt gga aaa cac cct gtg gtg gct att 1267

Ser Ala Ala Gly Leu Ala Leu Gly Gly Lys His Pro Val Val Ala Ile

375 380 385

tac tcc acg ttc ttg aac cgc gct ttt gat cag ctg ctc atg gat gtg 1315

Tyr Ser Thr Phe Leu Asn Arg Ala Phe Asp Gln Leu Leu Met Asp Val

390 395 400 405

ggc atg ctc aac cag cct gtt act ttg gtg ctt gat cgc tca ggt gtc 1363

Gly Met Leu Asn Gln Pro Val Thr Leu Val Leu Asp Arg Ser Gly Val

410 415 420

acg ggt tcg gat gga gcg agc cac aat ggc gtc tgg gat atg gcg ctg 1411

Thr Gly Ser Asp Gly Ala Ser His Asn Gly Val Trp Asp Met Ala Leu

425 430 435

acc tcg atc gtt cca ggc gtg cag gtg gcg gca cca cgt gat gag gat 1459

Thr Ser Ile Val Pro Gly Val Gln Val Ala Ala Pro Arg Asp Glu Asp

440 445 450

tcc ttg cgt gag ctg ctc aat gag gct att tcc atc gat gat ggc ccc 1507

Ser Leu Arg Glu Leu Leu Asn Glu Ala Ile Ser Ile Asp Asp Gly Pro

455 460 465

aca gtt gtg cgt ttc ccc aag ggc gac ttg cca act cca att gtt gct 1555

Thr Val Val Arg Phe Pro Lys Gly Asp Leu Pro Thr Pro Ile Val Ala

470 475 480 485

atc gac acc ttg gaa gac ggc gtg gat gtc ctc gca tat gaa gac gcc 1603

Ile Asp Thr Leu Glu Asp Gly Val Asp Val Leu Ala Tyr Glu Asp Ala

490 495 500

act gac gtt gaa tca acc gac gat gcg cca tca gtt ctc atc att gcg 1651

Thr Asp Val Glu Ser Thr Asp Asp Ala Pro Ser Val Leu Ile Ile Ala

505 510 515

gta ggc gag cgc gca act gtt gca ctt gac gtt gct tcc agg att aaa 1699

Val Gly Glu Arg Ala Thr Val Ala Leu Asp Val Ala Ser Arg Ile Lys

520 525 530

cag cac ggc gtg aac gtc acg gtt gtt gac ccc cgc tgg att gtc ccc 1747

Gln His Gly Val Asn Val Thr Val Val Asp Pro Arg Trp Ile Val Pro

535 540 545

atc ccg cag tcc ttg gtc gcg ctg tct gat gat cat gac ctc gtg atc 1795

Ile Pro Gln Ser Leu Val Ala Leu Ser Asp Asp His Asp Leu Val Ile

550 555 560 565

acc atc gaa gac ggc gtc atc cac ggc ggc gtg gga tcc ttg ctc tct 1843

Thr Ile Glu Asp Gly Val Ile His Gly Gly Val Gly Ser Leu Leu Ser

570 575 580

gat gcg ctt aac gcc tct gag gtg gat acc cct cgc cga caa atc gcc 1891

Asp Ala Leu Asn Ala Ser Glu Val Asp Thr Pro Arg Arg Gln Ile Ala

585 590 595

gtg ccc cag aag tac ctg gat cac gcg tcc cgc aat gaa gtg ctc gcc 1939

Val Pro Gln Lys Tyr Leu Asp His Ala Ser Arg Asn Glu Val Leu Ala

600 605 610

gat tat ggc ctc gac gcc gac ggc att gaa acc act gtt gtt gga tgg 1987

Asp Tyr Gly Leu Asp Ala Asp Gly Ile Glu Thr Thr Val Val Gly Trp

615 620 625

ctg gat tcc ctg ttc ggg gaa taaaaccctg cttatcgacg ccgccctcca 2038

Leu Asp Ser Leu Phe Gly Glu

630 635

<210>50

<211>636

<212>PRT

<213>谷氨酸棒状杆菌

<400>50

Met Gly Ile Leu Asn Ser Ile Ser Thr Pro Ala Asp Leu Lys Ala Leu

1 5 10 15

Asn Asp Glu Asp Leu Asp Ala Leu Ala Lys Glu Ile Arg Thr Phe Leu

20 25 30

Val Asp Lys Val Ala Ala Thr Gly Gly His Leu Gly Pro Asn Leu Gly

35 40 45

Val Val Glu Leu Thr Ile Gly Leu His Arg Val Phe Asp Ser Pro Gln

50 55 60

Asp Pro Ile Ile Phe Asp Thr Ser His Gln Ser Tyr Val His Lys Ile

65 70 75 80

Leu Thr Gly Arg Ala Lys Asp Phe Asp Ser Leu Arg Gln Lys Asp Gly

85 90 95

Leu Ser Gly Tyr Thr Cys Arg Ala Glu Ser Glu His Asp Trp Thr Glu

100 105 110

Ser Ser His Ala Ser Ala Ala Leu Ser Tyr Ala Asp Gly Leu Ser Lys

115 120 125

Ala Lys Gln Leu Asp Gly Asp Thr Thr His Ser Val Val Ala Val Val

130 135 140

Gly Asp Gly Ala Leu Thr Gly Gly Met Cys Trp Glu Ala Leu Asn Asn

145 150 155 160

Ile Ala Ala Gly Lys Asp Arg Lys Val Val Val Val Val Asn Asp Asn

165 170 175

Gly Arg Ser Tyr Ser Pro Thr Ile Gly Gly Phe Ala Glu Asn Leu Ala

180 185 190

Gly Leu Arg Met Gln Pro Phe Tyr Asp Arg Phe Met Glu Lys Gly Lys

195 200 205

Thr Ser Leu Lys Ser Met Gly Trp Val Gly Glu Arg Thr Phe Glu Ala

21 215 220

Leu His Ala Phe Lys Glu Gly Val Lys Ser Thr Val Ile Pro Thr Glu

225 230 235 240

Met Phe Pro Glu Leu Gly Met Lys Tyr Val Gly Pro Val Asp Gly His

245 250 255

Asn Gln Lys Ala Val Asp Asn Ala Leu Lys Tyr Ala His Asp Tyr Asp

260 265 270

Gly Pro Ile Ile Val His Met Val Thr Glu Lys Gly Arg Gly Tyr Ala

275 280 285

Pro Ala Glu Gln Asp Leu Asp Glu Leu Met His Ser Thr Gly Val Ile

290 295 300

Asp Pro Leu Thr Gly Ala Pro Lys Ser Ala Ser Lys Pro Gly Trp Thr

305 310 315 320

Ser Val Phe Ser Asp Glu Leu Val Lys Ile Gly Ala Gln Asn Glu Asn

325 330 335

Val Val Ala Ile Thr Ala Ala Met Ala Gly Pro Thr Gly Leu Ser Lys

340 345 350

Phe Glu Ala Asn Phe Pro Asn Arg Phe Phe Asp Val Gly Ile Ala Glu

355 360 365

Gln His Ala Val Thr Ser Ala Ala Gly Leu Ala Leu Gly Gly Lys His

370 375 380

Pro Val Val Ala Ile Tyr Ser Thr Phe Leu Asn Arg Ala Phe Asp Gln

385 390 395 400

Leu Leu Met Asp Val Gly Met Leu Asn Gln Pro Val Thr Leu Val Leu

405 410 415

Asp Arg Ser Gly Val Thr Gly Ser Asp Gly Ala Ser His Asn Gly Val

420 425 430

Trp Asp Met Ala Leu Thr Ser Ile Val Pro Gly Val Gln Val Ala Ala

435 440 445

Pro Arg Asp Glu Asp Ser Leu Arg Glu Leu Leu Asn Glu Ala Ile Ser

450 455 460

Ile Asp Asp Gly Pro Thr Val Val Arg Phe Pro Lys Gly Asp Leu Pro

465 470 475 480

Thr Pro Ile Val Ala Ile Asp Thr Leu Glu Asp Gly Val Asp Val Leu

485 490 495

Ala Tyr Glu Asp Ala Thr Asp Val Glu Ser Thr Asp Asp Ala Pro Ser

500 505 510

Val Leu Ile Ile Ala Val Gly Glu Arg Ala Thr Val Ala Leu Asp Val

515 520 525

Ala Ser Arg Ile Lys Gln His Gly Val Asn Val Thr Val Val Asp Pro

530 535 540

Arg Trp Ile Val Pro Ile Pro Gln Ser Leu Val Ala Leu Ser Asp Asp

545 550 555 560

His Asp Leu Val Ile Thr Ile Glu Asp Gly Val Ile His Gly Gly Val

565 570 575

Gly Ser Leu Leu Ser Asp Ala Leu Asn Ala Ser Glu Val Asp Thr Pro

580 585 590

Arg Arg Gln Ile Ala Val Pro Gln Lys Tyr Leu Asp His Ala Ser Arg

595 600 605

Asn Glu Val Leu Ala Asp Tyr Gly Leu Asp Ala Asp Gly Ile Glu Thr

610 615 620

Thr Val Val Gly Trp Leu Asp Ser Leu Phe Gly Glu

625 630 635

<210>51

<211>1489

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1459)

<223>RXA01569

<400>51

aaggcctaga gcagaccatc gattggtacc gcgaaaacga ggcctggtgg cgccctgcca 60

agaacaacgt cgaagctacc tacgctaagc agggacaata atg gaa tac ggt aaa 115

Met Glu Tyr Gly Lys

1 5

caa ctc acc tcc cac acc acc gac atc gaa ggc cta ctg gtt ttc gat 163

Gln Leu Thr Ser His Thr Thr Asp Ile Glu Gly Leu Leu Val Phe Asp

10 15 20

ttc ccc gtc cac ggc gac aac cgc ggc tgg ttc aag gaa aat tgg cag 211

Phe Pro Val His Gly Asp Asn Arg Gly Trp Phe Lys Glu Asn Trp Gln

25 30 35

cgc acc aag atg acc aac ctg ggg ctg ccc gat ttt ggc ccc gtc caa 259

Arg Thr Lys Met Thr Asn Leu Gly Leu Pro Asp Phe Gly Pro Val Gln

40 45 50

aac aac atg agt ttc aac gcc acc gcc ggc acg act cgc ggc atg cac 307

Asn Asn Met Ser Phe Asn Ala Thr Ala Gly Thr Thr Arg Gly Met His

55 60 65

gct gag ccg tgg gat aaa ttt gtg tcc gtc gcg gtg ggt tcc gtt ttc 355

Ala Glu Pro Trp Asp Lys Phe Val Ser Val Ala Val Gly Ser Val Phe

70 75 80 85

gga gct tgg gtg gat ctg cgc gcg ggc tcg agc acg tac ggt aac gtc 403

Gly Ala Trp Val Asp Leu Arg Ala Gly Ser Ser Thr Tyr Gly Asn Val

90 95 100

gta acg caa aaa att acc cct gac gtg gga gtt tac gtc ccg cgt ggt 451

Val Thr Gln Lys Ile Thr Pro Asp Val Gly Val Tyr Val Pro Arg Gly

105 110 115

gtg gca aac ggc ttc cag gcg ctc gag gac ggc acg ctg tac acc tac 499

Val Ala Asn Gly Phe Gln Ala Leu Glu Asp Gly Thr Leu Tyr Thr Tyr

120 125 130

ctc gtc aac gat cat tgg tcc ccc gac gcg cat tac gcc aac gtc aac 547

Leu Val Asn Asp His Trp Ser Pro Asp Ala His Tyr Ala Asn Val Asn

135 140 145

ctc aac atg atc gac tgg ccg ctg ccc atc acc gag atc tcc gaa aaa 595

Leu Asn Met Ile Asp Trp Pro Leu Pro Ile Thr Glu Ile Ser Glu Lys

150 155 160 165

gat aaa aaa cat cca gcg ctt atc gac gcc acc ccc ctg ccc gcc cgc 643

Asp Lys Lys His Pro Ala Leu Ile Asp Ala Thr Pro Leu Pro Ala Arg

170 175 180

aag gtt ctc gtg gtc ggc gcc ggc gga caa ctg gga acc gcg cta cgc 691

Lys Val Leu Val Val Gly Ala Gly Gly Gln Leu Gly Thr Ala Leu Arg

185 190 195

gcg cag ttc cca gac gcg gaa ttt gtc acg cgc caa gaa ctc gat atc 739

Ala Gln Phe Pro Asp Ala Glu Phe Val Thr Arg Gln Glu Leu Asp Ile

200 205 210

acc tca gat ctc acc gag gct cgc gcg tgg aaa caa tac tcc acc atc 787

Thr Ser Asp Leu Thr Glu Ala Arg Ala Trp Lys Gln Tyr Ser Thr Ile

215 220 225

ata aac gcc gcc gcc tac act gcc gtt gac cag gca gaa cac gac cgc 835

Ile Asn Ala Ala Ala Tyr Thr Ala Val Asp Gln Ala Glu His Asp Arg

230 235 240 245

gca gca gcg tgg gac atc aac gca gcg gca gtg gct aac ctc gcg acc 883

Ala Ala Ala Trp Asp Ile Asn Ala Ala Ala Val Ala Asn Leu Ala Thr

250 255 260

atc gcg cgc gac aac aac ctc acc ctc gtg cac gtg tcc tca gat tat 931

Ile Ala Arg Asp Asn Asn Leu Thr Leu Val His Val Ser Ser Asp Tyr

265 270 275

gtc ttc gac ggt gcg gcc gaa tcc tac gat gaa aac gca ccg ttt tcc 979

Val Phe Asp Gly Ala Ala Glu Ser Tyr Asp Glu Asn Ala Pro Phe Ser

280 285 290

cca ctc ggc gtg tac ggc caa tcc aaa gca gcc ggc gac atc gca gcc 1027

Pro Leu Gly Val Tyr Gly Gln Ser Lys Ala Ala Gly Asp Ile Ala Ala

295 300 305

acc acc gca ccg cgc cac tac att gtg cgc acc agc tgg gtg att ggc 1075

Thr Thr Ala Pro Arg His Tyr Ile Val Arg Thr Ser Trp Val Ile Gly

310 315 320 325

gat ggc aat aat ttt gtc cgc acc atg aaa tcc ctc gac gaa cgc ggc 1123

Asp Gly Asn Asn Phe Val Arg Thr Met Lys Ser Leu Asp Glu Arg Gly

330 335 340

atc gca cca tca gta gtt gat gat caa atc ggc cgc cta tcc ttc acc 1171

Ile Ala Pro Ser Val Val Asp Asp Gln Ile Gly Arg Leu Ser Phe Thr

345 350 355

gaa gac atc gca gcc ggc atc gcg cac ctt ttg gaa gtg ggt gca gca 1219

Glu Asp Ile Ala Ala Gly Ile Ala His Leu Leu Glu Val Gly Ala Ala

360 365 370

tat ggc acc tac aac ctc acc aac acc ggc gaa ccc gca agc tgg gcc 1267

Tyr Gly Thr Tyr Asn Leu Thr Asn Thr Gly Glu Pro Ala Ser Trp Ala

375 380 385

gat gtt gcc cgc gca gta ttt tcc gac ccc acc aaa gtt acc ggc gtg 1315

Asp Val Ala Arg Ala Val Phe Ser Asp Pro Thr Lys Val Thr Gly Val

390 395 400 405

agc acc gcc gag tac ttc gcc aac aaa gac gca gcg ccc cgc cca ctg 1363

Ser Thr Ala Glu Tyr Phe Ala Asn Lys Asp Ala Ala Pro Arg Pro Leu

410 415 420

aac tcc gtt ttg gat ctc ggc aaa atc gaa gcc acc gga ttt agc gca 1411

Asn Ser Val Leu Asp Leu Gly Lys Ile Glu Ala Thr Gly Phe Ser Ala

425 430 435

ccg acc tgg cag acc cgc ctc aac gac tac ctc aag gaa ctc tca aag 1459

Pro Thr Trp Gln Thr Arg Leu Asn Asp Tyr Leu Lys Glu Leu Ser Lys

440 445 450

tgaaaggcat catcctcgca ggtggctccg 1489

<210>52

<211>453

<212>PRT

<213>谷氨酸棒状杆菌

<400>52

Met Glu Tyr Gly Lys Gln Leu Thr Ser His Thr Thr Asp Ile Glu Gly

1 5 10 15

Leu Leu Val Phe Asp Phe Pro Val His Gly Asp Asn Arg Gly Trp Phe

20 25 30

Lys Glu Asn Trp Gln Arg Thr Lys Met Thr Asn Leu Gly Leu Pro Asp

35 40 45

Phe Gly Pro Val Gln Asn Asn Met Ser Phe Asn Ala Thr Ala Gly Thr

50 55 60

Thr Arg Gly Met His Ala Glu Pro Trp Asp Lys Phe Val Ser Val Ala

65 70 75 80

Val Gly Ser Val Phe Gly Ala Trp Val Asp Leu Arg Ala Gly Ser Ser

85 90 95

Thr Tyr Gly Asn Val Val Thr Gln Lys Ile Thr Pro Asp Val Gly Val

100 105 110

Tyr Val Pro Arg Gly Val Ala Asn Gly Phe Gln Ala Leu Glu Asp Gly

115 120 125

Thr Leu Tyr Thr Tyr Leu Val Asn Asp His Trp Ser Pro Asp Ala His

130 135 140

Tyr Ala Asn Val Asn Leu Asn Met Ile Asp Trp Pro Leu Pro Ile Thr

145 150 155 160

Glu Ile Ser Glu Lys Asp Lys Lys His Pro Ala Leu Ile Asp Ala Thr

165 170 175

Pro Leu Pro Ala Arg Lys Val Leu Val Val Gly Ala Gly Gly Gln Leu

180 185 190

Gly Thr Ala Leu Arg Ala Gln Phe Pro Asp Ala Glu Phe Val Thr Arg

195 200 205

Gln Glu Leu Asp Ile Thr Ser Asp Leu Thr Glu Ala Arg Ala Trp Lys

210 215 220

Gln Tyr Ser Thr Ile Ile Asn Ala Ala Ala Tyr Thr Ala Val Asp Gln

225 230 235 240

Ala Glu His Asp Arg Ala Ala Ala Trp Asp Ile Asn Ala Ala Ala Val

245 250 255

Ala Asn Leu Ala Thr Ile Ala Arg Asp Asn Asn Leu Thr Leu Val His

260 265 270

Val Ser Ser Asp Tyr Val Phe Asp Gly Ala Ala Glu Ser Tyr Asp Glu

275 280 285

Asn Ala Pro Phe Ser Pro Leu Gly Val Tyr Gly Gln Ser Lys Ala Ala

290 295 300

Gly Asp Ile Ala Ala Thr Thr Ala Pro Arg His Tyr Ile Val Arg Thr

305 310 315 320

Ser Trp Val Ile Gly Asp Gly Asn Asn Phe Val Arg Thr Met Lys Ser

325 330 335

Leu Asp Glu Arg Gly Ile Ala Pro Ser Val Val Asp Asp Gln Ile Gly

340 345 350

Arg Leu Ser Phe Thr Glu Asp Ile Ala Ala Gly Ile Ala His Leu Leu

355 360 365

Glu Val Gly Ala Ala Tyr Gly Thr Tyr Asn Leu Thr Asn Thr Gly Glu

370 375 380

Pro Ala Ser Trp Ala Asp Val Ala Arg Ala Val Phe Ser Asp Pro Thr

385 390 395 400

Lys Val Thr Gly Val Ser Thr Ala Glu Tyr Phe Ala Asn Lys Asp Ala

405 410 415

Ala Pro Arg Pro Leu Asn Ser Val Leu Asp Leu Gly Lys Ile Glu Ala

420 425 430

Thr Gly Phe Ser Ala Pro Thr Trp Gln Thr Arg Leu Asn Asp Tyr Leu

435 440 445

Lys Glu Leu Ser Lys

450

<210>53

<211>1552

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1522)

<223>RXA01693

<400>53

aggagactgg ctggattatt ggctggtttt cttgggaaat cgtcatgggc attaatccta 60

gtcccaacaa ttgccagaac ccgaacacta ggcttgaacc atg aat act gca cct 115

Met Asn Thr Ala Pro

1 5

ttc aaa ctc gaa gct gac ttc gca tca gcc ctg ccc acc atg gca gcc 163

Phe Lys Leu Glu Ala Asp Phe Ala Ser Ala Leu Pro Thr Met Ala Ala

10 15 20

ccc tgg caa ggt gag gaa gcc ccc aac cct gag ctc gtg att tta aat 211

Pro Trp Gln Gly Glu Glu Ala Pro Asn Pro Glu Leu Val Ile Leu Asn

25 30 35

gac gac ctc gcc tac agc ctc ggg ctt gat ccg aca tgg ctt cgc aca 259

Asp Asp Leu Ala Tyr Ser Leu Gly Leu Asp Pro Thr Trp Leu Arg Thr

40 45 50

cct gag ggc gtt caa ttt ctt ctc gga ctc aac ccc gag ccc tta aca 307

Pro Glu Gly Val Gln Phe Leu Leu Gly Leu Asn Pro Glu Pro Leu Thr

55 60 65

aaa gca gtt gcg cag gcc tat tcc ggc cac caa ttc gga cag ttt gtg 355

Lys Ala Val Ala Gln Ala Tyr Ser Gly His Gln Phe Gly Gln Phe Val

70 75 80 85

gca agc ctt ggt gat ggc cga gcg ctt ctt ctc ggc gaa gcc cgc tca 403

Ala Ser Leu Gly Asp Gly Arg Ala Leu Leu Leu Gly Glu Ala Arg Ser

90 95 100

gct gac ggc gta ctg cat gat atc cac ctc aaa gga tct gga cga acc 451

Ala Asp Gly Val Leu His Asp Ile His Leu Lys Gly Ser Gly Arg Thr

105 110 115

caa ttc tcc cga gga gcc gat gga cgc gcc gtc ctt ggc ccc gtc tta 499

Gln Phe Ser Arg Gly Ala Asp Gly Arg Ala Val Leu Gly Pro Val Leu

120 125 130

cgc gaa tac atc atc tcc gaa gcg atg cat gca ctt ggt gtt ccc acc 547

Arg Glu Tyr Ile Ile Ser Glu Ala Met His Ala Leu Gly Val Pro Thr

135 140 145

acc agg tca ctt gca gta att agc acc ggt agg aaa atc caa cga gga 595

Thr Arg Ser Leu Ala Val Ile Ser Thr Gly Arg Lys Ile Gln Arg Gly

150 155 160 165

agc gta gcc cca ggc gca gtc ctt gtt cga gta gca acc agc ctc att 643

Ser Val Ala Pro Gly Ala Val Leu Val Arg Val Ala Thr Ser Leu Ile

170 175 180

cga gtc gga tcc ttc caa tac tcc aac atc tct ggt ggc atc gaa cta 691

Arg Val Gly Ser Phe Gln Tyr Ser Asn Ile Ser Gly Gly Ile Glu Leu

185 190 195

tct caa cac ctg gcg aac tat acg atc acc agg cat ttc cct tcg ttg 739

Ser Gln His Leu Ala Asn Tyr Thr Ile Thr Arg His Phe Pro Ser Leu

200 205 210

gta gct gaa cta tcc gca cca acc ccc gca act tat gta tca ctg ttt 787

Val Ala Glu Leu Ser Ala Pro Thr Pro Ala Thr Tyr Val Ser Leu Phe

215 220 225

aaa gcg att ctt cag cgc caa gca gac acc gtt gga aaa tgg acc agg 835

Lys Ala Ile Leu Gln Arg Gln Ala Asp Thr Val Gly Lys Trp Thr Arg

230 235 240 245

ctg ggt ttc gtt cac gga gcc ctc aac aca gac aac acg ttg ata tcc 883

Leu Gly Phe Val His Gly Ala Leu Asn Thr Asp Asn Thr Leu Ile Ser

250 255 260

gga gaa act gtt gac tac ggc cca tgc gct ttc atg gag cgc tac cgt 931

Gly Glu Thr Val Asp Tyr Gly Pro Cys Ala Phe Met Glu Arg Tyr Arg

265 270 275

ggc gac gcg aaa ttt agc tcc atc gac act tat ggt cgc tac aaa ttt 979

Gly Asp Ala Lys Phe Ser Ser Ile Asp Thr Tyr Gly Arg Tyr Lys Phe

280 285 290

gaa aac caa cct atg atc ctc gga tgg aac atg gcc cgc ctc gta gaa 1027

Glu Asn Gln Pro Met Ile Leu Gly Trp Asn Met Ala Arg Leu Val Glu

295 300 305

acc ctc ctc cca ctc ctg ggc gcc aca cca gac gaa ggc atg aca gca 1075

Thr Leu Leu Pro Leu Leu Gly Ala Thr Pro Asp Glu Gly Met Thr Ala

310 315 320 325

gcc caa gaa gct ctc gta gaa ttc gat gac ctc tgc gaa caa gca atc 1123

Ala Gln Glu Ala Leu Val Glu Phe Asp Asp Leu Cys Glu Gln Ala Ile

330 335 340

cga aaa gaa ttc gcc act gca ctg ggc ctt gac gag tca gac acc ggc 1171

Arg Lys Glu Phe Ala Thr Ala Leu Gly Leu Asp Glu Ser Asp Thr Gly

345 350 355

acg gta gag cag ttc cgt gaa ctg ctc tac ctc cat aac ccc gac atc 1219

Thr Val Glu Gln Phe Arg Glu Leu Leu Tyr Leu His Asn Pro Asp Ile

360 365 370

acc acg ctg ctg cgc gca ctc acc gac aac acc gca cca ccg agt ggc 1267

Thr Thr Leu Leu Arg Ala Leu Thr Asp Asn Thr Ala Pro Pro Ser Gly

375 380 385

ttt gaa gca ttc gtt cac gac tgg aaa acc caa gac cca gat atc gaa 1315

Phe Glu Ala Phe Val His Asp Trp Lys Thr Gln Asp Pro Asp Ile Glu

390 395 400 405

gca atg cga gca gta aat cca ctt ttc att cca cgc aat cac ctc gtg 1363

Ala Met Arg Ala Val Asn Pro Leu Phe Ile Pro Arg Asn His Leu Val

410 415 420

gaa gct gct ctc gca gac gca gtt gaa ggg aat cta gaa aag ttc cac 1411

Glu Ala Ala Leu Ala Asp Ala Val Glu Gly Asn Leu Glu Lys Phe His

425 430 435

gaa ctc ctc gct gct gtc acc aat cct ttt gat cca act gcg ggc ccc 1459

Glu Leu Leu Ala Ala Val Thr Asn Pro Phe Asp Pro Thr Ala Gly Pro

440 445 450

gat gaa cta cgc ctg cca agc gaa gaa gga ttt gaa gaa gac tac atg 1507

Asp Glu Leu Arg Leu Pro Ser Glu Glu Gly Phe Glu Glu Asp Tyr Met

455 460 465

acc ttc tgc ggt acc taggacagat ggtggggcag acgtcggggt 1552

Thr Phe Cys Gly Thr

470

<210>54

<211>474

<212>PRT

<213>谷氨酸棒状杆菌

<400>54

Met Asn Thr Ala Pro Phe Lys Leu Glu Ala Asp Phe Ala Ser Ala Leu

1 5 10 15

Pro Thr Met Ala Ala Pro Trp Gln Gly Glu Glu Ala Pro Asn Pro Glu

20 25 30

Leu Val Ile Leu Asn Asp Asp Leu Ala Tyr Ser Leu Gly Leu Asp Pro

35 40 45

Thr Trp Leu Arg Thr Pro Glu Gly Val Gln Phe Leu Leu Gly Leu Asn

50 55 60

Pro Glu Pro Leu Thr Lys Ala Val Ala Gln Ala Tyr Ser Gly His Gln

65 70 75 80

Phe Gly Gln Phe Val Ala Ser Leu Gly Asp Gly Arg Ala Leu Leu Leu

85 90 95

Gly Glu Ala Arg Ser Ala Asp Gly Val Leu His Asp Ile His Leu Lys

100 105 110

Gly Ser Gly Arg Thr Gln Phe Ser Arg Gly Ala Asp Gly Arg Ala Val

115 120 125

Leu Gly Pro Val Leu Arg Glu Tyr Ile Ile Ser Glu Ala Met His Ala

130 135 140

Leu Gly Val Pro Thr Thr Arg Ser Leu Ala Val Ile Ser Thr Gly Arg

145 150 155 160

Lys Ile Gln Arg Gly Ser Val Ala Pro Gly Ala Val Leu Val Arg Val

165 170 175

Ala Thr Ser Leu Ile Arg Val Gly Ser Phe Gln Tyr Ser Asn Ile Ser

180 185 190

Gly Gly Ile Glu Leu Ser Gln His Leu Ala Asn Tyr Thr Ile Thr Arg

195 200 205

His Phe Pro Ser Leu Val Ala Glu Leu Ser Ala Pro Thr Pro Ala Thr

210 215 220

Tyr Val Ser Leu Phe Lys Ala Ile Leu Gln Arg Gln Ala Asp Thr Val

225 230 235 240

Gly Lys Trp Thr Arg Leu Gly Phe Val His Gly Ala Leu Asn Thr Asp

245 250 255

Asn Thr Leu Ile Ser Gly Glu Thr Val Asp Tyr Gly Pro Cys Ala Phe

260 265 270

Met Glu Arg Tyr Arg Gly Asp Ala Lys Phe Ser Ser Ile Asp Thr Tyr

275 280 285

Gly Arg Tyr Lys Phe Glu Asn Gln Pro Met Ile Leu Gly Trp Asn Met

290 295 300

Ala Arg Leu Val Glu Thr Leu Leu Pro Leu Leu Gly Ala Thr Pro Asp

305 310 315 320

Glu Gly Met Thr Ala Ala Gln Glu Ala Leu Val Glu Phe Asp Asp Leu

325 330 335

Cys Glu Gln Ala Ile Arg Lys Glu Phe Ala Thr Ala Leu Gly Leu Asp

340 345 350

Glu Ser Asp Thr Gly Thr Val Glu Gln Phe Arg Glu Leu Leu Tyr Leu

355 360 365

His Asn Pro Asp Ile Thr Thr Leu Leu Arg Ala Leu Thr Asp Asn Thr

370 375 380

Ala Pro Pro Ser Gly Phe Glu Ala Phe Val His Asp Trp Lys Thr Gln

385 390 395 400

Asp Pro Asp Ile Glu Ala Met Arg Ala Val Asn Pro Leu Phe Ile Pro

405 410 415

Arg Asn His Leu Val Glu Ala Ala Leu Ala Asp Ala Val Glu Gly Asn

420 425 430

Leu Glu Lys Phe His Glu Leu Leu Ala Ala Val Thr Asn Pro Phe Asp

435 440 445

Pro Thr Ala Gly Pro Asp Glu Leu Arg Leu Pro Ser Glu Glu Gly Phe

450 455 460

Glu Glu Asp Tyr Met Thr Phe Cys Gly Thr

465 470

<210>55

<211>1792

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1762)

<223>RXA01814

<400>55

tgttaagcca ccctactccg tgaattttgc cgtatctcgt gcgcacaatt gcttttgagg 60

ggaagatgaa gagaaagtat tggtgtttta aggagcaaac atg gca cat gaa cgc 115

Met Ala His Glu Arg

1 5

gcc ggg caa ctc gcc caa cca gaa gat ctc atc gat gtt gcg gaa ctg 163

Ala Gly Gln Leu Ala Gln Pro Glu Asp Leu Ile Asp Val Ala Glu Leu

10 15 20

gtc acc gca tat ttc acc cgc aag ccg gac gtg aac aac cct gat cag 211

Val Thr Ala Tyr Phe Thr Arg Lys Pro Asp Val Asn Asn Pro Asp Gln

25 30 35

cag gtc gct ttc ggc acc tcc gga cac cgt ggc tcc gcg ctg gac agc 259

Gln Val Ala Phe Gly Thr Ser Gly His Arg Gly Ser Ala Leu Asp Ser

40 45 50

gct ttc aac gag gac cac atc ctg gca acc acc cag gcg atc gtc gac 307

Ala Phe Asn Glu Asp His Ile Leu Ala Thr Thr Gln Ala Ile Val Asp

55 60 65

tac cgc aac cag cag cca aaa aac tgg gtc ggc ccg ctg ttt atc ggc 355

Tyr Arg Asn Gln Gln Pro Lys Asn Trp Val Gly Pro Leu Phe Ile Gly

70 75 80 85

cgc gat acg cac gcg ctg tcc gaa cca gcg atg atc agc gcg ctt gag 403

Arg Asp Thr His Ala Leu Ser Glu Pro Ala Met Ile Ser Ala Leu Glu

90 95 100

gtc ctc att gcc aac gac gtc gaa gtg ctt gtc gac gcc gac ggc cgc 451

Val Leu Ile Ala Asn Asp Val Glu Val Leu Val Asp Ala Asp Gly Arg

105 110 115

tac acc ccg acg ccc gca gtg tcc cac gcg atc cta cga cac aac gat 499

Tyr Thr Pro Thr Pro Ala Val Ser His Ala Ile Leu Arg His Asn Asp

120 125 130

ggc atc atc ctt ggc acc gca gga ccc tcc cgc ccc tac gcc gac ggc 547

Gly Ile Ile Leu Gly Thr Ala Gly Pro Ser Arg Pro Tyr Ala Asp Gly

135 140 145

atc gtg atc acc cca tcc cac aac cct cct cgt gat ggc gga ttc aaa 595

Ile Val Ile Thr Pro Ser His Asn Pro Pro Arg Asp Gly Gly Phe Lys

150 155 160 165

tac aac cca gcc aac ggt ggc cct gca gat acc gac gcc acc gac tgg 643

Tyr Asn Pro Ala Asn Gly Gly Pro Ala Asp Thr Asp Ala Thr Asp Trp

170 175 180

atc gcc aac cgc gcc aac gat att ctg cgc ggc gac ctt gca gac gtg 691

Ile Ala Asn Arg Ala Asn Asp Ile Leu Arg Gly Asp Leu Ala Asp Val

185 190 195

aag cga gtt cca gtt tcc ggt gtc ctc gac gag cgc acc act gcc tac 739

Lys Arg Val Pro Val Ser Gly Val Leu Asp Glu Arg Thr Thr Ala Tyr

200 205 210

gac ttc aag ggc att tac atc gct gac ctg cca aac gtg gtc aac atc 787

Asp Phe Lys Gly Ile Tyr Ile Ala Asp Leu Pro Asn Val Val Asn Ile

215 220 225

gat gcc atc cgc gaa gct ggt gtt cga atc ggc gca gac cca atg ggt 835

Asp Ala Ile Arg Glu Ala Gly Val Arg Ile Gly Ala Asp Pro Met Gly

230 235 240 245

ggc gca tcc gtg gat tac tgg ggt gcc atc gca gaa acc cat ggc ctc 883

Gly Ala Ser Val Asp Tyr Trp Gly Ala Ile Ala Glu Thr His Gly Leu

250 255 260

aac ctc acc gtg gtc aac cca cac gtt gat tcc acc ttc cgc ttc atg 931

Asn Leu Thr Val Val Asn Pro His Val Asp Ser Thr Phe Arg Phe Met

265 270 275

aca ttg gac acc gac ggc aag atc cgc atg gac tgc tcc agc cca cac 979

Thr Leu Asp Thr Asp Gly Lys Ile Arg Met Asp Cys Ser Ser Pro His

280 285 290

gca atg gca tcg ctg att gac aac cga gac aag ttc gat gtg gca acc 1027

Ala Met Ala Ser Leu Ile Asp Asn Arg Asp Lys Phe Asp Val Ala Thr

295 300 305

ggc aac gac gcc gac gcc gac cgc cac ggc atc gtc acc cca gac gct 1075

Gly Asn Asp Ala Asp Ala Asp Arg His Gly Ile Val Thr Pro Asp Ala

310 315 320 325

ggc ttg atg aac ccc aac cac tac ctc gca gta gca att gag tac ctc 1123

Gly Leu Met Asn Pro Asn His Tyr Leu Ala Val Ala Ile Glu Tyr Leu

330 335 340

ttt gct cac cgc cca ggt tgg tcc gca gat acc gca gtg ggc aaa acc 1171

Phe Ala His Arg Pro Gly Trp Ser Ala Asp Thr Ala Val Gly Lys Thr

345 350 355

ctg gtc agc tcc tcc atg atc gac cgc gtt gtg gcg cag ctt ggc cgc 1219

Leu Val Ser Ser Ser Met Ile Asp Arg Val Val Ala Gln Leu Gly Arg

360 365 370

acc ctc gtt gag gtt cca gtc gga ttc aag tgg ttt gtc cca ggt ttg 1267

Thr Leu Val Glu Val Pro Val Gly Phe Lys Trp Phe Val Pro Gly Leu

375 380 385

atc tcc ggc gaa atc gga ttc ggt ggt gaa gaa tcc gca ggt gca tcc 1315

Ile Ser Gly Glu Ile Gly Phe Gly Gly Glu Glu Ser Ala Gly Ala Ser

390 395 400 405

ttc ctc cgc atg gac ggc acc acc tgg tcc acc gac aag gac ggc ctc 1363

Phe Leu Arg Met Asp Gly Thr Thr Trp Ser Thr Asp Lys Asp Gly Leu

410 415 420

atc ctt gac ctc ctg gca gct gag atc att gca gta acc ggc aag acc 1411

Ile Leu Asp Leu Leu Ala Ala Glu Ile Ile Ala Val Thr Gly Lys Thr

425 430 435

cca tca cag cgc tac gca gaa ctc gcc gaa gaa ttc ggt gca cct gcc 1459

Pro Ser Gln Arg Tyr Ala Glu Leu Ala Glu Glu Phe Gly Ala Pro Ala

440 445 450

tac gcc cgc acc gat gca gaa gcc aac cga gaa caa aag gcc atc ctg 1507

Tyr Ala Arg Thr Asp Ala Glu Ala Asn Arg Glu Gln Lys Ala Ile Leu

455 460 465

aag gca ctg tcc cca gaa cag gtc acc gcc acc gaa cta gcc ggc gaa 1555

Lys Ala Leu Ser Pro Glu Gln Val Thr Ala Thr Glu Leu Ala Gly Glu

470 475 480 485

gca atc acc gct aag ctc acc gaa gct ccc ggc aat ggc gca gcc atc 1603

Ala Ile Thr Ala Lys Leu Thr Glu Ala Pro Gly Asn Gly Ala Ala Ile

490 495 500

gga gga cta aaa gtg acc acc gaa aac gcc tgg ttc gca gca cgc cca 1651

Gly Gly Leu Lys Val Thr Thr Glu Asn Ala Trp Phe Ala Ala Arg Pro

505 510 515

tcc ggc acc gaa gac aag tac aag atc tac gca gaa tcc ttc aag ggc 1699

Ser Gly Thr Glu Asp Lys Tyr Lys Ile Tyr Ala Glu Ser Phe Lys Gly

520 525 530

gaa gag cac ctc gcc cag gtt cag aag gaa gcc caa gcg ttg gtc agc 1747

Glu Glu His Leu Ala Gln Val Gln Lys Glu Ala Gln Ala Leu Val Ser

535 540 545

gaa gta ctc gga cag taaaactgcg gacttgctga caagtttcac 1792

Glu Val Leu Gly Gln

550

<210>56

<211>554

<212>PRT

<213>谷氨酸棒状杆菌

<400>56

Met Ala His Glu Arg Ala Gly Gln Leu Ala Gln Pro Glu Asp Leu Ile

1 5 10 15

Asp Val Ala Glu Leu Val Thr Ala Tyr Phe Thr Arg Lys Pro Asp Val

20 25 30

Asn Asn Pro Asp Gln Gln Val Ala Phe Gly Thr Ser Gly His Arg Gly

35 40 45

Ser Ala Leu Asp Ser Ala Phe Asn Glu Asp His Ile Leu Ala Thr Thr

50 55 60

Gln Ala Ile Val Asp Tyr Arg Asn Gln Gln Pro Lys Asn Trp Val Gly

65 70 75 80

Pro Leu Phe Ile Gly Arg Asp Thr His Ala Leu Ser Glu Pro Ala Met

85 90 95

Ile Ser Ala Leu Glu Val Leu Ile Ala Asn Asp Val Glu Val Leu Val

100 105 110

Asp Ala Asp Gly Arg Tyr Thr Pro Thr Pro Ala Val Ser His Ala Ile

115 120 125

Leu Arg His Asn Asp Gly Ile Ile Leu Gly Thr Ala Gly Pro Ser Arg

130 135 140

Pro Tyr Ala Asp Gly Ile Val Ile Thr Pro Ser His Asn Pro Pro Arg

145 150 155 160

Asp Gly Gly Phe Lys Tyr Asn Pro Ala Asn Gly Gly Pro Ala Asp Thr

165 170 175

Asp Ala Thr Asp Trp Ile Ala Asn Arg Ala Asn Asp Ile Leu Arg Gly

180 185 190

Asp Leu Ala Asp Val Lys Arg Val Pro Val Ser Gly Val Leu Asp Glu

195 200 205

Arg Thr Thr Ala Tyr Asp Phe Lys Gly Ile Tyr Ile Ala Asp Leu Pro

210 215 220

Asn Val Val Asn Ile Asp Ala Ile Arg Glu Ala Gly Val Arg Ile Gly

225 230 235 240

Ala Asp Pro Met Gly Gly Ala Ser Val Asp Tyr Trp Gly Ala Ile Ala

245 250 255

Glu Thr His Gly Leu Asn Leu Thr Val Val Asn Pro His Val Asp Ser

260 265 270

Thr Phe Arg Phe Met Thr Leu Asp Thr Asp Gly Lys Ile Arg Met Asp

275 280 285

Cys Ser Ser Pro His Ala Met Ala Ser Leu Ile Asp Asn Arg Asp Lys

290 295 300

Phe Asp Val Ala Thr Gly Asn Asp Ala Asp Ala Asp Arg His Gly Ile

305 310 315 320

Val Thr Pro Asp Ala Gly Leu Met Asn Pro Asn His Tyr Leu Ala Val

325 330 335

Ala Ile Glu Tyr Leu Phe Ala His Arg Pro Gly Trp Ser Ala Asp Thr

340 345 350

Ala Val Gly Lys Thr Leu Val Ser Ser Ser Met Ile Asp Arg Val Val

355 360 365

Ala Gln Leu Gly Arg Thr Leu Val Glu Val Pro Val Gly Phe Lys Trp

370 375 380

Phe Val Pro Gly Leu Ile Ser Gly Glu Ile Gly Phe Gly Gly Glu Glu

385 390 395 400

Ser Ala Gly Ala Ser Phe Leu Arg Met Asp Gly Thr Thr Trp Ser Thr

405 410 415

Asp Lys Asp Gly Leu Ile Leu Asp Leu Leu Ala Ala Glu Ile Ile Ala

420 425 430

Val Thr Gly Lys Thr Pro Ser Gln Arg Tyr Ala Glu Leu Ala Glu Glu

435 440 445

Phe Gly Ala Pro Ala Tyr Ala Arg Thr Asp Ala Glu Ala Asn Arg Glu

450 455 460

Gln Lys Ala Ile Leu Lys Ala Leu Ser Pro Glu Gln Val Thr Ala Thr

465 470 475 480

Glu Leu Ala Gly Glu Ala Ile Thr Ala Lys Leu Thr Glu Ala Pro Gly

485 490 495

Asn Gly Ala Ala Ile Gly Gly Leu Lys Val Thr Thr Glu Asn Ala Trp

500 505 510

Phe Ala Ala Arg Pro Ser Gly Thr Glu Asp Lys Tyr Lys Ile Tyr Ala

515 520 525

Glu Ser Phe Lys Gly Glu Glu His Leu Ala Gln Val Gln Lys Glu Ala

530 535 540

Gln Ala Leu Val Ser Glu Val Leu Gly Gln

545 550

<210>57

<211>1120

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1090)

<223>RXA01882

<400>57

accctttttc caccttggct gattttgatg cccttgttac cgatgaccac acgctagatt 60

ttccagtttt gcccgaccac aactttcagg tggtaacccc atg atc atc aca ttc 115

Met Ile Ile Thr Phe

1 5

acc cca aac ccg agt att gat tcc acg ctg tcg ctc ggc gaa gag ctc 163

Thr Pro Asn Pro Ser Ile Asp Ser Thr Leu Ser Leu Gly Glu Glu Leu

10 15 20

tcc cgt gga tcc gtc caa cga ctt gat tcc gtc acc gct gtc gca ggt 211

Ser Arg Gly Ser Val Gln Arg Leu Asp Ser Val Thr Ala Val Ala Gly

25 30 35

ggt aaa ggc atc aat gtc gcc cac gct gtc ttg ctt gcg ggc ttt gaa 259

Gly Lys Gly Ile Asn Val Ala His Ala Val Leu Leu Ala Gly Phe Glu

40 45 50

acc ttg gct gtg ttc cca gcc ggc aag ctc gac ccc ttc gtc cca ctg 307

Thr Leu Ala Val Phe Pro Ala Gly Lys Leu Asp Pro Phe Val Pro Leu

55 60 65

gtc cgc gac atc ggc ttg ccc gtg gaa act gtt gtg atc aac aag aac 355

Val Arg Asp Ile Gly Leu Pro Val Glu Thr Val Val Ile Asn Lys Asn

70 75 80 85

gtc cgc acc aac acc aca gtc acc gaa ccg gac ggc acc acc acc aag 403

Val Arg Thr Asn Thr Thr Val Thr Glu Pro Asp Gly Thr Thr Thr Lys

90 95 100

ctc aac ggc ccc ggc gcg ccg ctc agc gag cag aag ctc cgt agc ttg 451

Leu Asn Gly Pro Gly Ala Pro Leu Ser Glu Gln Lys Leu Arg Ser Leu

105 110 115

gaa aag gtg ctt atc gac gcg ctc cgc ccc gaa gtc acc tgg gtt gtc 499

Glu Lys Val Leu Ile Asp Ala Leu Arg Pro Glu Val Thr Trp Val Val

120 125 130

ctg gcg ggc tcg ctg cca cca ggg gca cca gtt gac tgg tac gcg cgt 547

Leu Ala Gly Ser Leu Pro Pro Gly Ala Pro Val Asp Trp Tyr Ala Arg

135 140 145

ctc acc gcg ttg atc cat tca gca cgc cct gac gtt cgc gtg gct gtc 595

Leu Thr Ala Leu Ile His Ser Ala Arg Pro Asp Val Arg Val Ala Val

150 155 160 165

gat acc tca gac aag cca ctg atg gcg ttg ggc gag agc ttg gat aca 643

Asp Thr Ser Asp Lys Pro Leu Met Ala Leu Gly Glu Ser Leu Asp Thr

170 175 180

cct ggc gct gct ccg aac ctg att aag cca aat ggt ctg gaa ctg ggc 691

Pro Gly Ala Ala Pro Asn Leu Ile Lys Pro Asn Gly Leu Glu Leu Gly

185 190 195

cag ctg gct aac act gat ggt gaa gag ctg gag gcg cgt gct gcg caa 739

Gln Leu Ala Asn Thr Asp Gly Glu Glu Leu Glu Ala Arg Ala Ala Gln

200 205 210

ggc gat tac gac gcc atc atc gca gct gcg gac gta ctg gtt aac cgt 787

Gly Asp Tyr Asp Ala Ile Ile Ala Ala Ala Asp Val Leu Val Asn Arg

215 220 225

ggc atc gaa cag gtg ctt gtc acc ttg ggt gcc gca gga gcg gtg ttg 835

Gly Ile Glu Gln Val Leu Val Thr Leu Gly Ala Ala Gly Ala Val Leu

230 235 240 245

gtc aac gca gaa ggt gcg tgg act gct act tct cca aag att gat gtt 883

Val Asn Ala Glu Gly Ala Trp Thr Ala Thr Ser Pro Lys Ile Asp Val

250 255 260

gta tcc acc gtt gga gct gga gac tgt gct ctt gca ggt ttt gtt atg 931

Val Ser Thr Val Gly Ala Gly Asp Cys Ala Leu Ala Gly Phe Val Met

265 270 275

gca cgt tcc cag aag aaa aca ctg gag gaa tct ctg ctg aat gcc gtg 979

Ala Arg Ser Gln Lys Lys Thr Leu Glu Glu Ser Leu Leu Asn Ala Val

280 285 290

tct tac ggc tcg act gcg gcg tct ctt cct ggc act acc att cct cgt 1027

Ser Tyr Gly Ser Thr Ala Ala Ser Leu Pro Gly Thr Thr Ile Pro Arg

295 300 305

cct gac caa ctc gcc aca gct ggt gca acg gtc acc caa gtc aaa gga 1075

Pro Asp Gln Leu Ala Thr Ala Gly Ala Thr Val Thr Gln Val Lys Gly

310 315 320 325

ttg aaa gaa tca gca tgaatagcgt aaataattcc tcgcttgtcc 1120

Leu Lys Glu Ser Ala

330

<210>58

<211>330

<212>PRT

<213>谷氨酸棒状杆菌

<400>58

Met Ile Ile Thr Phe Thr Pro Asn Pro Ser Ile Asp Ser Thr Leu Ser

1 5 10 15

Leu Gly Glu Glu Leu Ser Arg Gly Ser Val Gln Arg Leu Asp Ser Val

20 25 30

Thr Ala Val Ala Gly Gly Lys Gly Ile Asn Val Ala His Ala Val Leu

35 40 45

Leu Ala Gly Phe Glu Thr Leu Ala Val Phe Pro Ala Gly Lys Leu Asp

50 55 60

Pro Phe Val Pro Leu Val Arg Asp Ile Gly Leu Pro Val Glu Thr Val

65 70 75 80

Val Ile Asn Lys Asn Val Arg Thr Asn Thr Thr Val Thr Glu Pro Asp

85 90 95

Gly Thr Thr Thr Lys Leu Asn Gly Pro Gly Ala Pro Leu Ser Glu Gln

100 105 110

Lys Leu Arg Ser Leu Glu Lys Val Leu Ile Asp Ala Leu Arg Pro Glu

115 120 125

Val Thr Trp Val Val Leu Ala Gly Ser Leu Pro Pro Gly Ala Pro Val

130 135 140

Asp Trp Tyr Ala Arg Leu Thr Ala Leu Ile His Ser Ala Arg Pro Asp

145 150 155 160

Val Arg Val Ala Val Asp Thr Ser Asp Lys Pro Leu Met Ala Leu Gly

165 170 175

Glu Ser Leu Asp Thr Pro Gly Ala Ala Pro Asn Leu Ile Lys Pro Asn

180 185 190

Gly Leu Glu Leu Gly Gln Leu Ala Asn Thr Asp Gly Glu Glu Leu Glu

195 200 205

Ala Arg Ala Ala Gln Gly Asp Tyr Asp Ala Ile Ile Ala Ala Ala Asp

210 215 220

Val Leu Val Asn Arg Gly Ile Glu Gln Val Leu Val Thr Leu Gly Ala

225 230 235 240

Ala Gly Ala Val Leu Val Asn Ala Glu Gly Ala Trp Thr Ala Thr Ser

245 250 255

Pro Lys Ile Asp Val Val Ser Thr Val Gly Ala Gly Asp Cys Ala Leu

260 265 270

Ala Gly Phe Val Met Ala Arg Ser Gln Lys Lys Thr Leu Glu Glu Ser

275 280 285

Leu Leu Asn Ala Val Ser Tyr Gly Ser Thr Ala Ala Ser Leu Pro Gly

290 295 300

Thr Thr Ile Pro Arg Pro Asp Gln Leu Ala Thr Ala Gly Ala Thr Val

305 310 315 320

Thr Gln Val Lys Gly Leu Lys Glu Ser Ala

325 330

<210>59

<211>1141

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1111)

<223>RXA01887

<400>59

catctttaca ggaaacccct tgacggcatc aatgggtggt atctagtatc tactagaacg 60

ttatagtaga acgttctagt aaaacttgga aggatgaaaa atg tca gtc aaa ctt 115

Met Ser Val Lys Leu

1 5

gcc ctc atc ggt gct gga cgc atc gga tca aat cac gca cgc ctg atc 163

Ala Leu Ile Gly Ala Gly Arg Ile Gly Ser Asn His Ala Arg Leu Ile

10 15 20

aca aac cac gtg atc ggc tct gaa ctg gtc gcc gtc gtt gac cca act 211

Thr Asn His Val Ile Gly Ser Glu Leu Val Ala Val Val Asp Pro Thr

25 30 35

ccc aac gca gaa acc ctc gct gat gaa ttg ggc gcc gtt gcg ttc tct 259

Pro Asn Ala Glu Thr Leu Ala Asp Glu Leu Gly Ala Val Ala Phe Ser

40 45 50

aac cca gat gac gtc ctg acc cgc gat gac att gac gcg gtt ttg att 307

Asn Pro Asp Asp Val Leu Thr Arg Asp Asp Ile Asp Ala Val Leu Ile

55 60 65

gct aca cca gca cga acc cac gcg gat ctc gtg gtc aaa gca gcg gca 355

Ala Thr Pro Ala Arg Thr His Ala Asp Leu Val Val Lys Ala Ala Ala

70 75 80 85

gcg ggc aag cac gtg ttt gtg gaa aag ccc atg gcc gtc acc ctc gag 403

Ala Gly Lys His Val Phe Val Glu Lys Pro Met Ala Val Thr Leu Glu

90 95 100

gac gca gat cgt gcc atc aac gca gca cgc gaa gca aac act gtc ctg 451

Asp Ala Asp Arg Ala Ile Asn Ala Ala Arg Glu Ala Asn Thr Val Leu

105 110 115

cag gtg ggc ttc aat cgt cgt ttc gcg gca ggt ttc gct gca gca cgc 499

Gln Val Gly Phe Asn Arg Arg Phe Ala Ala Gly Phe Ala Ala Ala Arg

120 125 130

gca cgc att gac gca ggc gat atc ggc acc cca cag ctg ctt cgt tcc 547

Ala Arg Ile Asp Ala Gly Asp Ile Gly Thr Pro Gln Leu Leu Arg Ser

135 140 145

gtg acc cgc gat cca gga cca ttc acc gct gac ccc aac aag atc cct 595

Val Thr Arg Asp Pro Gly Pro Phe Thr Ala Asp Pro Asn Lys Ile Pro

150 155 160 165

cag tgg acc atc ttc ctg gaa acc ctc atc cac gat ttc gat gcg ctg 643

Gln Trp Thr Ile Phe Leu Glu Thr Leu Ile His Asp Phe Asp Ala Leu

170 175 180

tgc tac ctc aac cca ggc gca acc cca gtg gaa gta acc gct cac gct 691

Cys Tyr Leu Asn Pro Gly Ala Thr Pro Val Glu Val Thr Ala His Ala

185 190 195

gat tgc ctc gtc gtt cca gaa gct gct ggc act ggc ttc ctc gac acc 739

Asp Cys Leu Val Val Pro Glu Ala Ala Gly Thr Gly Phe Leu Asp Thr

200 205 210

gca gtg gtg act gtc cgt ttt gat aac gga gca att ggt act gca gaa 787

Ala Val Val Thr Val Arg Phe Asp Asn Gly Ala Ile Gly Thr Ala Glu

215 220 225

gca agc ttc agc gca gcc tat ggt tat gac gtt cgc ggt gaa gtc ttc 835

Ala Ser Phe Ser Ala Ala Tyr Gly Tyr Asp Val Arg Gly Glu Val Phe

230 235 240 245

gga tcc aag ggc atg atg acc gca ggc gac gcg cgc gca acc aac atg 883

Gly Ser Lys Gly Met Met Thr Ala Gly Asp Ala Arg Ala Thr Asn Met

250 255 260

act ttc tac ggc gct gag ggc atc gcg gct gcc acc tta cgc gcg gat 931

Thr Phe Tyr Gly Ala Glu Gly Ile Ala Ala Ala Thr Leu Arg Ala Asp

265 270 275

acc gat ctg ctc tcc gat gct tac cga gct gaa ttc caa gct ttc gtc 979

Thr Asp Leu Leu Ser Asp Ala Tyr Arg Ala Glu Phe Gln Ala Phe Val

280 285 290

gac tcc atc cgt acc aac acc cct tcc aag gtt cca ggc gaa gct gca 1027

Asp Ser Ile Arg Thr Asn Thr Pro Ser Lys Val Pro Gly Glu Ala Ala

295 300 305

cgc acc gca cta ctc atc gca ctc ggc gcc atc cga agc gta gaa acc 1075

Arg Thr Ala Leu Leu Ile Ala Leu Gly Ala Ile Arg Ser Val Glu Thr

310 315 320 325

ggc gca acc atc aac ctt gct gaa agc atc gag gtt taaccatgac 1121

Gly Ala Thr Ile Asn Leu Ala Glu Ser Ile Glu Val

330 335

ttttaaactc gcagcatgcg 1141

<210>60

<211>337

<212>PRT

<213>谷氨酸棒状杆菌

<400>60

Met Ser Val Lys Leu Ala Leu Ile Gly Ala Gly Arg Ile Gly Ser Asn

1 5 10 15

His Ala Arg Leu Ile Thr Asn His Val Ile Gly Ser Glu Leu Val Ala

20 25 30

Val Val Asp Pro Thr Pro Asn Ala Glu Thr Leu Ala Asp Glu Leu Gly

35 40 45

Ala Val Ala Phe Ser Asn Pro Asp Asp Val Leu Thr Arg Asp Asp Ile

50 55 60

Asp Ala Val Leu Ile Ala Thr Pro Ala Arg Thr His Ala Asp Leu Val

65 70 75 80

Val Lys Ala Ala Ala Ala Gly Lys His Val Phe Val Glu Lys Pro Met

85 90 95

Ala Val Thr Leu Glu Asp Ala Asp Arg Ala Ile Asn Ala Ala Arg Glu

100 105 110

Ala Asn Thr Val Leu Gln Val Gly Phe Asn Arg Arg Phe Ala Ala Gly

115 120 125

Phe Ala Ala Ala Arg Ala Arg Ile Asp Ala Gly Asp Ile Gly Thr Pro

130 135 140

Gln Leu Leu Arg Ser Val Thr Arg Asp Pro Gly Pro Phe Thr Ala Asp

145 150 155 160

Pro Asn Lys Ile Pro Gln Trp Thr Ile Phe Leu Glu Thr Leu Ile His

165 170 175

Asp Phe Asp Ala Leu Cys Tyr Leu Asn Pro Gly Ala Thr Pro Val Glu

180 185 190

Val Thr Ala His Ala Asp Cys Leu Val Val Pro Glu Ala Ala Gly Thr

195 200 205

Gly Phe Leu Asp Thr Ala Val Val Thr Val Arg Phe Asp Asn Gly Ala

210 215 220

Ile Gly Thr Ala Glu Ala Ser Phe Ser Ala Ala Tyr Gly Tyr Asp Val

225 230 235 240

Arg Gly Glu Val Phe Gly Ser Lys Gly Met Met Thr Ala Gly Asp Ala

245 250 255

Arg Ala Thr Asn Met Thr Phe Tyr Gly Ala Glu Gly Ile Ala Ala Ala

260 265 270

Thr Leu Arg Ala Asp Thr Asp Leu Leu Ser Asp Ala Tyr Arg Ala Glu

275 280 285

Phe Gln Ala Phe Val Asp Ser Ile Arg Thr Asn Thr Pro Ser Lys Val

290 295 300

Pro Gly Glu Ala Ala Arg Thr Ala Leu Leu Ile Ala Leu Gly Ala Ile

305 310 315 320

Arg Ser Val Glu Thr Gly Ala Thr Ile Asn Leu Ala Glu Ser Ile Glu

325 330 335

Val

<210>61

<211>3853

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(3823)

<223>RXA02056

<400>61

gtggcattta atggcctgac acgttatttt taggagaact gtcaacaaat taatgctaca 60

actggggctt aggcataatc agccaacgac caacgttaca gtg gat aaa aca aag 115

Val Asp Lys Thr Lys

1 5

ctc aat aaa ccc tca aga agc aag gaa aag agg cga gta cct gcc gtg 163

Leu Asn Lys Pro Ser Arg Ser Lys Glu Lys Arg Arg Val Pro Ala Val

10 15 20

agc agc gct agt act ttc ggc cag aat gcg tgg ctg gta gac gag atg 211

Ser Ser Ala Ser Thr Phe Gly Gln Asn Ala Trp Leu Val Asp Glu Met

25 30 35

ttc cag cag ttc cag aag gac ccc aag tcc gtg gac aag gaa tgg aga 259

Phe Gln Gln Phe Gln Lys Asp Pro Lys Ser Val Asp Lys Glu Trp Arg

40 45 50

gaa ctc ttt gag gcg cag ggg gga cca aat act acc ccc gct aca aca 307

Glu Leu Phe Glu Ala Gln Gly Gly Pro Asn Thr Thr Pro Ala Thr Thr

55 60 65

gaa gca cag cct tca gcg ccc aag gag tct gcg aaa cca gca cca aag 355

Glu Ala Gln Pro Ser Ala Pro Lys Glu Ser Ala Lys Pro Ala Pro Lys

70 75 80 85

gct gcc cct gca gcc aag gca gca ccg cgc gta gaa acc aag ccg gcc 403

Ala Ala Pro Ala Ala Lys Ala Ala Pro Arg Val Glu Thr Lys Pro Ala

90 95 100

gac aag acc gcc cct aag gcc aag gag tcc tca gtg cca cag caa cct 451

Asp Lys Thr Ala Pro Lys Ala Lys Glu Ser Ser Val Pro Gln Gln Pro

105 110 115

aag ctt ccg gag cca gga caa acc cca atc agg ggt att ttc aag tcc 499

Lys Leu Pro Glu Pro Gly Gln Thr Pro Ile Arg Gly Ile Phe Lys Ser

120 125 130

atc gcg aag aac atg gat atc tcc ctg gaa atc cca acc gca acc tcg 547

Ile Ala Lys Asn Met Asp Ile Ser Leu Glu Ile Pro Thr Ala Thr Ser

135 140 145

gtt cgc gat atg cca gct cgc ctc atg ttc gaa aac cgc gcg atg gtc 595

Val Arg Asp Met Pro Ala Arg Leu Met Phe Glu Asn Arg Ala Met Val

150 155 160 165

aac gat cag ctc aag cgc acc cgc ggt ggc aag atc tcc ttc acc cac 643

Asn Asp Gln Leu Lys Arg Thr Arg Gly Gly Lys Ile Ser Phe Thr His

170 175 180

atc att ggc tac gcc atg gtg aag gca gtc atg gct cac ccg gac atg 691

Ile Ile Gly Tyr Ala Met Val Lys Ala Val Met Ala His Pro Asp Met

185 190 195

aac aac tcc tac gac gtc atc gac ggc aag cca acc ctg atc gtg cct 739

Asn Asn Ser Tyr Asp Val Ile Asp Gly Lys Pro Thr Leu Ile Val Pro

200 205 210

gag cac atc aac ctg ggc ctt gct atc gac ctt cct cag aag gac ggc 787

Glu His Ile Asn Leu Gly Leu Ala Ile Asp Leu Pro Gln Lys Asp Gly

215 220 225

tcc cgc gca ctt gtc gta gca gcc atc aag gaa acc gag aag atg aac 835

Ser Arg Ala Leu Val Val Ala Ala Ile Lys Glu Thr Glu Lys Met Asn

230 235 240 245

ttc tcc gag ttc ctc gca gcc tac gaa gac atc gtg gca cgc tcc cgc 883

Phe Ser Glu Phe Leu Ala Ala Tyr Glu Asp Ile Val Ala Arg Ser Arg

250 255 260

aag ggc aag ctc acc atg gat gac tac cag ggc gtt acc gtt tcc ttg 931

Lys Gly Lys Leu Thr Met Asp Asp Tyr Gln Gly Val Thr Val Ser Leu

265 270 275

acc aac cca ggt ggc atc ggt acc cgc cac tct gtt cca cgt cta acc 979

Thr Asn Pro Gly Gly Ile Gly Thr Arg His Ser Val Pro Arg Leu Thr

280 285 290

aag ggc cag ggc acc atc atc ggt gtc ggt tcc atg gat tac cca gca 1027

Lys Gly Gln Gly Thr Ile Ile Gly Val Gly Ser Met Asp Tyr Pro Ala

295 300 305

gag ttc cag ggc gct tca gaa gac cgc ctt gca gag ctc ggc gtt ggc 1075

Glu Phe Gln Gly Ala Ser Glu Asp Arg Leu Ala Glu Leu Gly Val Gly

310 315 320 325

aaa ctt gtc acc atc acc tcc acc tac gat cac cgc gtg atc cag ggt 1123

Lys Leu Val Thr Ile Thr Ser Thr Tyr Asp His Arg Val Ile Gln Gly

330 335 340

gct gtg tcc ggt gaa ttc ctg cgc acc atg tct cgc ctg ctc acc gat 1171

Ala Val Ser Gly Glu Phe Leu Arg Thr Met Ser Arg Leu Leu Thr Asp

345 350 355

gat tcc ttc tgg gat gag atc ttc gac gca atg aac gtt cct tac acc 1219

Asp Ser Phe Trp Asp Glu Ile Phe Asp Ala Met Asn Val Pro Tyr Thr

360 365 370

cca atg cgt tgg gca cag gac gtt cca aac acc ggt gtt gat aag aac 1267

Pro Met Arg Trp Ala Gln Asp Val Pro Asn Thr Gly Val Asp Lys Asn

375 380 385

acc cgc gtc atg cag ctc att gag gca tac cgc tcc cgt gga cac ctc 1315

Thr Arg Val Met Gln Leu Ile Glu Ala Tyr Arg Ser Arg Gly His Leu

390 395 400 405

atc gct gac acc aac cca ctt tca tgg gtt cag cct ggc atg cca gtt 1363

Ile Ala Asp Thr Asn Pro Leu Ser Trp Val Gln Pro Gly Met Pro Val

410 415 420

cca gac cac cgc gac ctc gac atc gag acc cac aac ctg acc atc tgg 1411

Pro Asp His Arg Asp Leu Asp Ile Glu Thr His Asn Leu Thr Ile Trp

425 430 435

gat ctg gac cgt acc ttc aac gtc ggt ggc ttc ggc ggc aag gag acc 1459

Asp Leu Asp Arg Thr Phe Asn Val Gly Gly Phe Gly Gly Lys Glu Thr

440 445 450

atg acc ctg cgc gag gta ctg tcc cgc ctc cgc gct gcg tac acc ctc 1507

Met Thr Leu Arg Glu Val Leu Ser Arg Leu Arg Ala Ala Tyr Thr Leu

455 460 465

aag gtc ggc tcc gaa tac acc cac atc ctg gac cgc gac gag cgc acc 1555

Lys Val Gly Ser Glu Tyr Thr His Ile Leu Asp Arg Asp Glu Arg Thr

470 475 480 485

tgg ctg cag gac cgc ctc gag gcc gga atg cca aag cca acc cag gca 1603

Trp Leu Gln Asp Arg Leu Glu Ala Gly Met Pro Lys Pro Thr Gln Ala

490 495 500

gag cag aag tac atc ctg cag aag ctg aac gcc gcg gag gct ttc gag 1651

Glu Gln Lys Tyr Ile Leu Gln Lys Leu Asn Ala Ala Glu Ala Phe Glu

505 510 515

aac ttc ctg cag acc aag tac gtc ggc cag aag cgc ttc tcc ctc gaa 1699

Asn Phe Leu Gln Thr Lys Tyr Val Gly Gln Lys Arg Phe Ser Leu Glu

520 525 530

ggt gca gaa gca ctt atc cca ctg atg gac tcc gcc atc gac acc gcc 1747

Gly Ala Glu Ala Leu Ile Pro Leu Met Asp Ser Ala Ile Asp Thr Ala

535 540 545

gca ggc caa ggc ctc gac gaa gtt gtc atc ggt atg cca cac cgt ggt 1795

Ala Gly Gln Gly Leu Asp Glu Val Val Ile Gly Met Pro His Arg Gly

550 555 560 565

cgc ctc aac gtg ctg ttc aac atc gtg ggc aag cca ctg gca tcc atc 1843

Arg Leu Asn Val Leu Phe Asn Ile Val Gly Lys Pro Leu Ala Ser Ile

570 575 580

ttc aac gag ttt gaa ggc caa atg gag cag ggc cag atc ggt ggc tcc 1891

Phe Asn Glu Phe Glu Gly Gln Met Glu Gln Gly Gln Ile Gly Gly Ser

585 590 595

ggt gac gtg aag tac cac ctc ggt tcc gaa ggc cag cac ctg cag atg 1939

Gly Asp Val Lys Tyr His Leu Gly Ser Glu Gly Gln His Leu Gln Met

600 605 610

ttc ggc gac ggc gag atc aag gtc tcc ctg act gct aac ccg tcc cac 1987

Phe Gly Asp Gly Glu Ile Lys Val Ser Leu Thr Ala Asn Pro Ser His

615 620 625

ctg gaa gct gtt aac cca gtg atg gaa ggt atc gtc cgc gca aag cag 2035

Leu Glu Ala Val Asn Pro Val Met Glu Gly Ile Val Arg Ala Lys Gln

630 635 640 645

gac tac ctg gac aag ggc gta gac ggc aag act gtt gtg cca ctg ctg 2083

Asp Tyr Leu Asp Lys Gly Val Asp Gly Lys Thr Val Val Pro Leu Leu

650 655 660

ctc cac ggt gac gct gca ttc gca ggc ctg ggc atc gtg cca gaa acc 2131

Leu His Gly Asp Ala Ala Phe Ala Gly Leu Gly Ile Val Pro Glu Thr

665 670 675

atc aac ctg gct aag ctg cgt ggc tac gac gtc ggc ggc acc atc cac 2179

Ile Asn Leu Ala Lys Leu Arg Gly Tyr Asp Val Gly Gly Thr Ile His

680 685 690

atc gtg gtg aac aac cag atc ggc ttc acc acc acc cca gac tcc agc 2227

Ile Val Val Asn Asn Gln Ile Gly Phe Thr Thr Thr Pro Asp Ser Ser

695 700 705

cgc tcc atg cac tac gca acc gac tac gcc aag gca ttc ggc tgc cca 2275

Arg Ser Met His Tyr Ala Thr Asp Tyr Ala Lys Ala Phe Gly Cys Pro

710 715 720 725

gtc ttc cac gtc aac ggc gac gac cca gag gca gtt gtc tgg gtt ggc 2323

Val Phe His Val Asn Gly Asp Asp Pro Glu Ala Val Val Trp Val Gly

730 735 740

cag ctg gcc acc gag tac cgt cgt cgc ttc ggc aag gac gtc ttc atc 2371

Gln Leu Ala Thr Glu Tyr Arg Arg Arg Phe Gly Lys Asp Val Phe Ile

745 750 755

gac ctc gtc tgc tac cgc ctc cgc ggc cac aac gaa gct gat gat cct 2419

Asp Leu Val Cys Tyr Arg Leu Arg Gly His Asn Glu Ala Asp Asp Pro

760 765 770

tcc atg acc cag cca aag atg tat gag ctc atc acc ggc cgc gag acc 2467

Ser Met Thr Gln Pro Lys Met Tyr Glu Leu Ile Thr Gly Arg Glu Thr

775 780 785

gtt cgt gct cag tac acc gaa gac ctg ctc gga cgt gga gac ctc tcc 2515

Val Arg Ala Gln Tyr Thr Glu Asp Leu Leu Gly Arg Gly Asp Leu Ser

790 795 800 805

aac gaa gat gca gaa gca gtc gtc cgc gac ttc cac gac cag atg gaa 2563

Asn Glu Asp Ala Glu Ala Val Val Arg Asp Phe His Asp Gln Met Glu

810 815 820

tct gtg ttc aac gaa gtc aag gaa ggc ggc aag aag cag gct gag gca 2611

Ser Val Phe Asn Glu Val Lys Glu Gly Gly Lys Lys Gln Ala Glu Ala

825 830 835

cag acc ggc atc acc ggc tcc cag aag ctt cca cac ggc ctt gag acc 2659

Gln Thr Gly Ile Thr Gly Ser Gln Lys Leu Pro His Gly Leu Glu Thr

840 845 850

aac atc tcc cgt gaa gag ctc ctg gaa ctg gga cag gct ttc gcc aac 2707

Asn Ile Ser Arg Glu Glu Leu Leu Glu Leu Gly Gln Ala Phe Ala Asn

855 860 865

acc cca gaa ggc ttc aac tac cac cca cgt gtg gct ccc gtt gct aag 2755

Thr Pro Glu Gly Phe Asn Tyr His Pro Arg Val Ala Pro Val Ala Lys

870 875 880 885

aag cgc gtc tcc tct gtc acc gaa ggt ggc atc gac tgg gca tgg ggc 2803

Lys Arg Val Ser Ser Val Thr Glu Gly Gly Ile Asp Trp Ala Trp Gly

890 895 900

gag ctc ctc gcc ttc ggt tcc ctg gct aac tcc ggc cgc ttg gtt cgc 2851

Glu Leu Leu Ala Phe Gly Ser Leu Ala Asn Ser Gly Arg Leu Val Arg

905 910 915

ctt gca ggt gaa gat tcc cgc cgc ggt acc ttc acc cag cgc cac gca 2899

Leu Ala Gly Glu Asp Ser Arg Arg Gly Thr Phe Thr Gln Arg His Ala

920 925 930

gtt gcc atc gac cca gcg acc gct gaa gag ttc aac cca ctc cac gag 2947

Val Ala Ile Asp Pro Ala Thr Ala Glu Glu Phe Asn Pro Leu His Glu

935 940 945

ctt gca cag tcc aag ggc aac aac ggt aag ttc ctg gtc tac aac tcc 2995

Leu Ala Gln Ser Lys Gly Asn Asn Gly Lys Phe Leu Val Tyr Asn Ser

950 955 960 965

gca ctg acc gag tac gca ggc atg ggc ttc gag tac ggc tac tcc gta 3043

Ala Leu Thr Glu Tyr Ala Gly Met Gly Phe Glu Tyr Gly Tyr Ser Val

970 975 980

gga aac gaa gac tcc atc gtt gca tgg gaa gca cag ttc ggc gac ttc 3091

Gly Asn Glu Asp Ser Ile Val Ala Trp Glu Ala Gln Phe Gly Asp Phe

985 990 995

gcc aac ggc gct cag acc atc atc gat gag tac gtc tcc tca ggc gaa 3139

Ala Asn Gly Ala Gln Thr Ile Ile Asp Glu Tyr Val Ser Ser Gly Glu

1000 1005 1010

gct aag tgg ggc cag acc tcc aag ctg atc ctt ctg ctg cct cac ggc 3187

Ala Lys Trp Gly Gln Thr Ser Lys Leu Ile Leu Leu Leu Pro His Gly

1015 1020 1025

tac gaa ggc cag ggc cca gac cac tct tcc gca cgt atc gag cgc ttc 3235

Tyr Glu Gly Gln Gly Pro Asp His Ser Ser Ala Arg Ile Glu Arg Phe

1030 1035 1040 1045

ctg cag ctg tgc gct gag ggt tcc atg act gtt gct cag cca tcc acc 3283

Leu Gln Leu Cys Ala Glu Gly Ser Met Thr Val Ala Gln Pro Ser Thr

1050 1055 1060

cca gca aac cac ttc cac cta ctg cgt cgt cac gct ctg tcc gac ctg 3331

Pro Ala Asn His Phe His Leu Leu Arg Arg His Ala Leu Ser Asp Leu

1065 1070 1075

aag cgt cca ctg gtt atc ttc acc ccg aag tcc atg ctg cgt aac aag 3379

Lys Arg Pro Leu Val Ile Phe Thr Pro Lys Ser Met Leu Arg Asn Lys

1080 1085 1090

gct gct gcc tcc gca cca gaa gac ttc act gag gtc acc aag ttc cag 3427

Ala Ala Ala Ser Ala Pro Glu Asp Phe Thr Glu Val Thr Lys Phe Gln

1095 1100 1105

tcc gtg atc aac gat cca aac gtt gca gat gca gcc aag gtg aag aag 3475

Ser Val Ile Asn Asp Pro Asn Val Ala Asp Ala Ala Lys Val Lys Lys

1110 1115 1120 1125

gtc atg ctg gtc tcc ggc aag ctg tac tac gaa ttg gca aag cgc aag 3523

Val Met Leu Val Ser Gly Lys Leu Tyr Tyr Glu Leu Ala Lys Arg Lys

1130 1135 1140

gag aag gac gga cgc gac gac atc gcg atc gtt cgt atc gaa atg ctc 3571

Glu Lys Asp Gly Arg Asp Asp Ile Ala Ile Val Arg Ile Glu Met Leu

1145 1150 1155

cac cca att ccg ttc aac cgc atc tcc gag gct ctt gcc ggc tac cct 3619

His Pro Ile Pro Phe Asn Arg Ile Ser Glu Ala Leu Ala Gly Tyr Pro

1160 1165 1170

aac gct gag gaa gtc ctc ttc gtt cag gat gag cca gca aac cag ggc 3667

Asn Ala Glu Glu Val Leu Phe Val Gln Asp Glu Pro Ala Asn Gln Gly

1175 1180 1185

cca tgg ccg ttc tac cag gag cac ctc cca gag ctg atc ccg aac atg 3715

Pro Trp Pro Phe Tyr Gln Glu His Leu Pro Glu Leu Ile Pro Asn Met

1190 1195 1200 1205

cca aag atg cgc cgc gtt tcc cgc cgc gct cag tcc tcc acc gca act 3763

Pro Lys Met Arg Arg Val Ser Arg Arg Ala Gln Ser Ser Thr Ala Thr

1210 1215 1220

ggt gtt gcc aag gtg cac cag ctg gag gag aag cag ctt atc gac gag 3811

Gly Val Ala Lys Val His Gln Leu Glu Glu Lys Gln Leu Ile Asp Glu

1225 1230 1235

gct ttc gag gct taagtcttta tagtcctgca ctagcctaga 3853

Ala Phe Glu Ala

1240

<210>62

<211>1241

<212>PRT

<213>谷氨酸棒状杆菌

<400>62

Val Asp Lys Thr Lys Leu Asn Lys Pro Ser Arg Ser Lys Glu Lys Arg

1 5 10 15

Arg Val Pro Ala Val Ser Ser Ala Ser Thr Phe Gly Gln Asn Ala Trp

20 25 30

Leu Val Asp Glu Met Phe Gln Gln Phe Gln Lys Asp Pro Lys Ser Val

35 40 45

Asp Lys Glu Trp Arg Glu Leu Phe Glu Ala Gln Gly Gly Pro Asn Thr

50 55 60

Thr Pro Ala Thr Thr Glu Ala Gln Pro Ser Ala Pro Lys Glu Ser Ala

65 70 75 80

Lys Pro Ala Pro Lys Ala Ala Pro Ala Ala Lys Ala Ala Pro Arg Val

85 90 95

Glu Thr Lys Pro Ala Asp Lys Thr Ala Pro Lys Ala Lys Glu Ser Ser

100 105 110

Val Pro Gln Gln Pro Lys Leu Pro Glu Pro Gly Gln Thr Pro Ile Arg

115 120 125

Gly Ile Phe Lys Ser Ile Ala Lys Asn Met Asp Ile Ser Leu Glu Ile

130 135 140

Pro Thr Ala Thr Ser Val Arg Asp Met Pro Ala Arg Leu Met Phe Glu

145 150 155 160

Asn Arg Ala Met Val Asn Asp Gln Leu Lys Arg Thr Arg Gly Gly Lys

165 170 175

Ile Ser Phe Thr His Ile Ile Gly Tyr Ala Met Val Lys Ala Val Met

180 185 190

Ala His Pro Asp Met Asn Asn Ser Tyr Asp Val Ile Asp Gly Lys Pro

195 200 205

Thr Leu Ile Val Pro Glu His Ile Asn Leu Gly Leu Ala Ile Asp Leu

210 215 220

Pro Gln Lys Asp Gly Ser Arg Ala Leu Val Val Ala Ala Ile Lys Glu

225 230 235 240

Thr Glu Lys Met Asn Phe Ser Glu Phe Leu Ala Ala Tyr Glu Asp Ile

245 250 255

Val Ala Arg Ser Arg Lys Gly Lys Leu Thr Met Asp Asp Tyr Gln Gly

260 265 270

Val Thr Val Ser Leu Thr Asn Pro Gly Gly Ile Gly Thr Arg His Ser

275 280 285

Val Pro Arg Leu Thr Lys Gly Gln Gly Thr Ile Ile Gly Val Gly Ser

290 295 300

Met Asp Tyr Pro Ala Glu Phe Gln Gly Ala Ser Glu Asp Arg Leu Ala

305 310 315 320

Glu Leu Gly Val Gly Lys Leu Val Thr Ile Thr Ser Thr Tyr Asp His

325 330 335

Arg Val Ile Gln Gly Ala Val Ser Gly Glu Phe Leu Arg Thr Met Ser

340 345 350

Arg Leu Leu Thr Asp Asp Ser Phe Trp Asp Glu Ile Phe Asp Ala Met

355 360 365

Asn Val Pro Tyr Thr Pro Met Arg Trp Ala Gln Asp Val Pro Asn Thr

370 375 380

Gly Val Asp Lys Asn Thr Arg Val Met Gln Leu Ile Glu Ala Tyr Arg

385 390 395 400

Ser Arg Gly His Leu Ile Ala Asp Thr Asn Pro Leu Ser Trp Val Gln

405 410 415

Pro Gly Met Pro Val Pro Asp His Arg Asp Leu Asp Ile Glu Thr His

420 425 430

Asn Leu Thr Ile Trp Asp Leu Asp Arg Thr Phe Asn Val Gly Gly Phe

435 440 445

Gly Gly Lys Glu Thr Met Thr Leu Arg Glu Val Leu Ser Arg Leu Arg

450 455 460

Ala Ala Tyr Thr Leu Lys Val Gly Ser Glu Tyr Thr His Ile Leu Asp

465 470 475 480

Arg Asp Glu Arg Thr Trp Leu Gln Asp Arg Leu Glu Ala Gly Met Pro

485 490 495

Lys Pro Thr Gln Ala Glu Gln Lys Tyr Ile Leu Gln Lys Leu Asn Ala

500 505 510

Ala Glu Ala Phe Glu Asn Phe Leu Gln Thr Lys Tyr Val Gly Gln Lys

515 520 525

Arg Phe Ser Leu Glu Gly Ala Glu Ala Leu Ile Pro Leu Met Asp Ser

530 535 540

Ala Ile Asp Thr Ala Ala Gly Gln Gly Leu Asp Glu Val Val Ile Gly

545 550 555 560

Met Pro His Arg Gly Arg Leu Asn Val Leu Phe Asn Ile Val Gly Lys

565 570 575

Pro Leu Ala Ser Ile Phe Asn Glu Phe Glu Gly Gln Met Glu Gln Gly

580 585 590

Gln Ile Gly Gly Ser Gly Asp Val Lys Tyr His Leu Gly Ser Glu Gly

595 600 605

Gln His Leu Gln Met Phe Gly Asp Gly Glu Ile Lys Val Ser Leu Thr

610 615 620

Ala Asn Pro Ser His Leu Glu Ala Val Asn Pro Val Met Glu Gly Ile

625 630 635 640

Val Arg Ala Lys Gln Asp Tyr Leu Asp Lys Gly Val Asp Gly Lys Thr

645 650 655

Val Val Pro Leu Leu Leu His Gly Asp Ala Ala Phe Ala Gly Leu Gly

660 665 670

Ile Val Pro Glu Thr Ile Asn Leu Ala Lys Leu Arg Gly Tyr Asp Val

675 680 685

Gly Gly Thr Ile His Ile Val Val Asn Asn Gln Ile Gly Phe Thr Thr

690 695 700

Thr Pro Asp Ser Ser Arg Ser Met His Tyr Ala Thr Asp Tyr Ala Lys

705 710 715 720

Ala Phe Gly Cys Pro Val Phe His Val Asn Gly Asp Asp Pro Glu Ala

725 730 735

Val Val Trp Val Gly Gln Leu Ala Thr Glu Tyr Arg Arg Arg Phe Gly

740 745 750

Lys Asp Val Phe Ile Asp Leu Val Cys Tyr Arg Leu Arg Gly His Asn

755 760 765

Glu Ala Asp Asp Pro Ser Met Thr Gln Pro Lys Met Tyr Glu Leu Ile

770 775 780

Thr Gly Arg Glu Thr Val Arg Ala Gln Tyr Thr Glu Asp Leu Leu Gly

785 790 795 800

Arg Gly Asp Leu Ser Asn Glu Asp Ala Glu Ala Val Val Arg Asp Phe

805 810 815

His Asp Gln Met Glu Ser Val Phe Asn Glu Val Lys Glu Gly Gly Lys

820 825 830

Lys Gln Ala Glu Ala Gln Thr Gly Ile Thr Gly Ser Gln Lys Leu Pro

835 840 845

His Gly Leu Glu Thr Asn Ile Ser Arg Glu Glu Leu Leu Glu Leu Gly

850 855 860

Gln Ala Phe Ala Asn Thr Pro Glu Gly Phe Asn Tyr His Pro Arg Val

865 870 875 880

Ala Pro Val Ala Lys Lys Arg Val Ser Ser Val Thr Glu Gly Gly Ile

885 890 895

Asp Trp Ala Trp Gly Glu Leu Leu Ala Phe Gly Ser Leu Ala Asn Ser

900 905 910

Gly Arg Leu Val Arg Leu Ala Gly Glu Asp Ser Arg Arg Gly Thr Phe

915 920 925

Thr Gln Arg His Ala Val Ala Ile Asp Pro Ala Thr Ala Glu Glu Phe

930 935 940

Asn Pro Leu His Glu Leu Ala Gln Ser Lys Gly Asn Asn Gly Lys Phe

945 950 955 960

Leu Val Tyr Asn Ser Ala Leu Thr Glu Tyr Ala Gly Met Gly Phe Glu

965 970 975

Tyr Gly Tyr Ser Val Gly Asn Glu Asp Ser Ile Val Ala Trp Glu Ala

980 985 990

Gln Phe Gly Asp Phe Ala Asn Gly Ala Gln Thr Ile Ile Asp Glu Tyr

995 1000 1005

Val Ser Ser Gly Glu Ala Lys Trp Gly Gln Thr Ser Lys Leu Ile Leu

1010 1015 1020

Leu Leu Pro His Gly Tyr Glu Gly Gln Gly Pro Asp His Ser Ser Ala

1025 1030 1035 1040

Arg Ile Glu Arg Phe Leu Gln Leu Cys Ala Glu Gly Ser Met Thr Val

1045 1050 1055

Ala Gln Pro Ser Thr Pro Ala Asn His Phe His Leu Leu Arg Arg His

1060 1065 1070

Ala Leu Ser Asp Leu Lys Arg Pro Leu Val Ile Phe Thr Pro Lys Ser

1075 1080 1085

Met Leu Arg Asn Lys Ala Ala Ala Ser Ala Pro Glu Asp Phe Thr Glu

1090 1095 1100

Val Thr Lys Phe Gln Ser Val Ile Asn Asp Pro Asn Val Ala Asp Ala

1105 1110 1115 1120

Ala Lys Val Lys Lys Val Met Leu Val Ser Gly Lys Leu Tyr Tyr Glu

1125 1130 1135

Leu Ala Lys Arg Lys Glu Lys Asp Gly Arg Asp Asp Ile Ala Ile Val

1140 1145 1150

Arg Ile Glu Met Leu His Pro Ile Pro Phe Asn Arg Ile Ser Glu Ala

1155 1160 1165

Leu Ala Gly Tyr Pro Asn Ala Glu Glu Val Leu Phe Val Gln Asp Glu

1170 1175 1180

Pro Ala Asn Gln Gly Pro Trp Pro Phe Tyr Gln Glu His Leu Pro Glu

1185 1190 1195 1200

Leu Ile Pro Asn Met Pro Lys Met Arg Arg Val Ser Arg Arg Ala Gln

1205 1210 1215

Ser Ser Thr Ala Thr Gly Val Ala Lys Val His Gln Leu Glu Glu Lys

1220 1225 1230

Gln Leu Ile Asp Glu Ala Phe Glu Ala

1235 1240

<210>63

<211>1381

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1351)

<223>RXA02063

<400>63

atctaaaaac agttgcgcag cgataccgaa atgggggcat taaaaggggc tatcattcgg 60

accccaaaac gatgtttaga caatttgtta cccagctttc atg cgg gat agt tat 115

Met Arg Asp Ser Tyr

1 5

ttt gcc ttt atg gtt aag ggt gtg aag ggt aga cca aat gtt cta gca 163

Phe Ala Phe Met Val Lys Gly Val Lys Gly Arg Pro Asn Val Leu Ala

10 15 20

atc gtt ctc gca ggt ggc gag ggc aaa cga ctt ttt ccg ttg acg gag 211

Ile Val Leu Ala Gly Gly Glu Gly Lys Arg Leu Phe Pro Leu Thr Glu

25 30 35

gac cga gct aag cct gcg gtc cca ttc ggc gga act tac aga ttg atc 259

Asp Arg Ala Lys Pro Ala Val Pro Phe Gly Gly Thr Tyr Arg Leu Ile

40 45 50

gac ttt gtt ttg tcg aac ctg gtg aac tcc gga ttc ctc aag atc gcg 307

Asp Phe Val Leu Ser Asn Leu Val Asn Ser Gly Phe Leu Lys Ile Ala

55 60 65

gta ctg act cag tac aag tcg cat tca ttg gat agg cat att tca ttg 355

Val Leu Thr Gln Tyr Lys Ser His Ser Leu Asp Arg His Ile Ser Leu

70 75 80 85

tcg tgg aac gtg tct ggt cca acg ggg cag tac att gct tct gtt cct 403

Ser Trp Asn Val Ser Gly Pro Thr Gly Gln Tyr Ile Ala Ser Val Pro

90 95 100

gcg cag cag cgc ctg ggc aag cga tgg ttc act ggt tcc gcg gat gca 451

Ala Gln Gln Arg Leu Gly Lys Arg Trp Phe Thr Gly Ser Ala Asp Ala

105 110 115

att ttg cag tct ctg aac ttg atc tct gat gag aaa ccg gat tat gtc 499

Ile Leu Gln Ser Leu Asn Leu Ile Ser Asp Glu Lys Pro Asp Tyr Val

120 125 130

atc gtt ttc ggc gcg gac cac gtg tat cgc atg gac cca agc cag atg 547

Ile Val Phe Gly Ala Asp His Val Tyr Arg Met Asp Pro Ser Gln Met

135 140 145

cta gat gag cac att gca tct ggt cgc gcg gtg tct gtg gca ggt att 595

Leu Asp Glu His Ile Ala Ser Gly Arg Ala Val Ser Val Ala Gly Ile

150 155 160 165

cgc gtt cca cgt gag gaa gca act gcg ttt ggt tgc atc cag tcc gat 643

Arg Val Pro Arg Glu Glu Ala Thr Ala Phe Gly Cys Ile Gln Ser Asp

170 175 180

gtc gac ggc aac ata acc gag ttc ttg gaa aag cca gct gac cct ccg 691

Val Asp Gly Asn Ile Thr Glu Phe Leu Glu Lys Pro Ala Asp Pro Pro

185 190 195

gga acc cct gat gat cct gac atg act tac gcg tcg atg ggt aac tac 739

Gly Thr Pro Asp Asp Pro Asp Met Thr Tyr Ala Ser Met Gly Asn Tyr

200 205 210

att ttc acc act gaa gcc ctg atc cag gcg ctg aaa gat gat gaa aat 787

Ile Phe Thr Thr Glu Ala Leu Ile Gln Ala Leu Lys Asp Asp Glu Asn

215 220 225

aac gag aac agt gat cat gac atg ggc gga gac atc att ccg tac ttc 835

Asn Glu Asn Ser Asp His Asp Met Gly Gly Asp Ile Ile Pro Tyr Phe

230 235 240 245

gtc tct cgc aat gat gcg cat gtt tat gat ttc tcc gga aac att gtt 883

Val Ser Arg Asn Asp Ala His Val Tyr Asp Phe Ser Gly Asn Ile Val

250 255 260

cct ggc gca act gag cgt gac aag ggc tac tgg cgc gac gtc ggt acc 931

Pro Gly Ala Thr Glu Arg Asp Lys Gly Tyr Trp Arg Asp Val Gly Thr

265 270 275

att gat gcg ttc tac gag tgc cac atg gac ctg att tcc gtg cac cca 979

Ile Asp Ala Phe Tyr Glu Cys His Met Asp Leu Ile Ser Val His Pro

280 285 290

atc ttc aat ctg tat aac tct gag tgg cca atc cac acg acc tct gaa 1027

Ile Phe Asn Leu Tyr Asn Ser Glu Trp Pro Ile His Thr Thr Ser Glu

295 300 305

ggt aac ttg cct ccg gct aag ttc gtt cgg ggc ggt atc gcg cag tcg 1075

Gly Asn Leu Pro Pro Ala Lys Phe Val Arg Gly Gly Ile Ala Gln Ser

310 315 320 325

tcg atg gtg tct tca ggt tcc atc att tct gct ggg act gtt cgc aac 1123

Ser Met Val Ser Ser Gly Ser Ile Ile Ser Ala Gly Thr Val Arg Asn

330 335 340

tcc gtg ctg tcc aac aac gtt gtc gtc gaa gag ggc gca acg gtg gaa 1171

Ser Val Leu Ser Asn Asn Val Val Val Glu Glu Gly Ala Thr Val Glu

345 350 355

ggt gca gtg ctg atg cca ggc gtg cgc atc ggt aag ggt gct gtt gtc 1219

Gly Ala Val Leu Met Pro Gly Val Arg Ile Gly Lys Gly Ala Val Val

360 365 370

cgc cat gcg att ctg gac aag aac gtg gtt gtc cgc gac gga gag ctc 1267

Arg His Ala Ile Leu Asp Lys Asn Val Val Val Arg Asp Gly Glu Leu

375 380 385

atc ggt gtc gac caa gtg cgc gat gcg cag cgc ttc aag gtg agc gcc 1315

Ile Gly Val Asp Gln Val Arg Asp Ala Gln Arg Phe Lys Val Ser Ala

390 395 400 405

ggc ggc gtc gtg gtt gtc ggt aag aac cag gta gtc taaacgggaa 1361

Gly Gly Val Val Val Val Gly Lys Asn Gln Val Val

410 415

agggacctta aaaggtccct 1381

<210>64

<211>417

<212>PRT

<213>谷氨酸棒状杆菌

<400>64

Met Arg Asp Ser Tyr Phe Ala Phe Met Val Lys Gly Val Lys Gly Arg

1 5 10 15

Pro Asn Val Leu Ala Ile Val Leu Ala Gly Gly Glu Gly Lys Arg Leu

20 25 30

Phe Pro Leu Thr Glu Asp Arg Ala Lys Pro Ala Val Pro Phe Gly Gly

35 40 45

Thr Tyr Arg Leu Ile Asp Phe Val Leu Ser Asn Leu Val Asn Ser Gly

50 55 60

Phe Leu Lys Ile Ala Val Leu Thr Gln Tyr Lys Ser His Ser Leu Asp

65 70 75 80

Arg His Ile Ser Leu Ser Trp Asn Val Ser Gly Pro Thr Gly Gln Tyr

85 90 95

Ile Ala Ser Val Pro Ala Gln Gln Arg Leu Gly Lys Arg Trp Phe Thr

100 105 110

Gly Ser Ala Asp Ala Ile Leu Gln Ser Leu Asn Leu Ile Ser Asp Glu

115 120 125

Lys Pro Asp Tyr Val Ile Val Phe Gly Ala Asp His Val Tyr Arg Met

130 135 140

Asp Pro Ser Gln Met Leu Asp Glu His Ile Ala Ser Gly Arg Ala Val

145 150 155 160

Ser Val Ala Gly Ile Arg Val Pro Arg Glu Glu Ala Thr Ala Phe Gly

165 170 175

Cys Ile Gln Ser Asp Val Asp Gly Asn Ile Thr Glu Phe Leu Glu Lys

180 185 190

Pro Ala Asp Pro Pro Gly Thr Pro Asp Asp Pro Asp Met Thr Tyr Ala

195 200 205

Ser Met Gly Asn Tyr Ile Phe Thr Thr Glu Ala Leu Ile Gln Ala Leu

210 215 220

Lys Asp Asp Glu Asn Asn Glu Asn Ser Asp His Asp Met Gly Gly Asp

225 230 235 240

Ile Ile Pro Tyr Phe Val Ser Arg Asn Asp Ala His Val Tyr Asp Phe

245 250 255

Ser Gly Asn Ile Val Pro Gly Ala Thr Glu Arg Asp Lys Gly Tyr Trp

260 265 270

Arg Asp Val Gly Thr Ile Asp Ala Phe Tyr Glu Cys His Met Asp Leu

275 280 285

Ile Ser Val His Pro Ile Phe Asn Leu Tyr Asn Ser Glu Trp Pro Ile

290 295 300

His Thr Thr Ser Glu Gly Asn Leu Pro Pro Ala Lys Phe Val Arg Gly

305 310 315 320

Gly Ile Ala Gln Ser Ser Met Val Ser Ser Gly Ser Ile Ile Ser Ala

325 330 335

Gly Thr Val Arg Asn Ser Val Leu Ser Asn Asn Val Val Val Glu Glu

340 345 350

Gly Ala Thr Val Glu Gly Ala Val Leu Met Pro Gly Val Arg Ile Gly

355 360 365

Lys Gly Ala Val Val Arg His Ala Ile Leu Asp Lys Asn Val Val Val

370 375 380

Arg Asp Gly Glu Leu Ile Gly Val Asp Gln Val Arg Asp Ala Gln Arg

385 390 395 400

Phe Lys Val Ser Ala Gly Gly Val Val Val Val Gly Lys Asn Gln Val

405 410 415

Val

<210>65

<211>2680

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2650)

<223>RXA02100

<400>65

cacatcaccg tttcacttcc gggtgtctca acagatgaac ttctgaaccg ataactcatt 60

ctggtagcag gaaccccaat cttaggctta gtttagggct ttg aaa cca gta ggc 115

Leu Lys Pro Val Gly

1 5

aca gta cag ttc aag aat cag ctc cca act caa ctc cag cca ctc gtg 163

Thr Val Gln Phe Lys Asn Gln Leu Pro Thr Gln Leu Gln Pro Leu Val

10 15 20

aag ctg gcg cat aat ctg cgc tgg tca tgg cgt gag gaa gca aag cag 211

Lys Leu Ala His Asn Leu Arg Trp Ser Trp Arg Glu Glu Ala Lys Gln

25 30 35

ctt ttc cgc gac atc gac cca gaa ttg tgg gaa cag ctc gac gaa gat 259

Leu Phe Arg Asp Ile Asp Pro Glu Leu Trp Glu Gln Leu Asp Glu Asp

40 45 50

cct aag cag atg ctt ctc cag gca cca gca act cgt ttg cag ttc ctc 307

Pro Lys Gln Met Leu Leu Gln Ala Pro Ala Thr Arg Leu Gln Phe Leu

55 60 65

gca ggc gat gat gag tac cta ggt cga gtc aac gcc gag gag caa aac 355

Ala Gly Asp Asp Glu Tyr Leu Gly Arg Val Asn Ala Glu Glu Gln Asn

70 75 80 85

ctc agc gaa tac ctc agc gac aag ctg tgg tac cag gac acc gca gat 403

Leu Ser Glu Tyr Leu Ser Asp Lys Leu Trp Tyr Gln Asp Thr Ala Asp

90 95 100

gca acc gat gct gtc gga gat cca ctc gtt gcg tac ttc tcc atg gag 451

Ala Thr Asp Ala Val Gly Asp Pro Leu Val Ala Tyr Phe Ser Met Glu

105 110 115

ttt ggc att cac cca agc ctg cca atc tac tct ggc gga ctt ggt gtg 499

Phe Gly Ile His Pro Ser Leu Pro Ile Tyr Ser Gly Gly Leu Gly Val

120 125 130

ctt gcg ggc gag aac atg aag tct gca tct gac ttg ggt gtg cca ctg 547

Leu Ala Gly Glu Asn Met Lys Ser Ala Ser Asp Leu Gly Val Pro Leu

135 140 145

atc ggt gtt ggt ttg ctc tac acc cac ggc tac ttc acc cag tca ctg 595

Ile Gly Val Gly Leu Leu Tyr Thr His Gly Tyr Phe Thr Gln Ser Leu

150 155 160 165

tcc ggt gac ggt tgg cag cag gaa gag tac aag tac cac gat cca gca 643

Ser Gly Asp Gly Trp Gln Gln Glu Glu Tyr Lys Tyr His Asp Pro Ala

170 175 180

gaa ctg ccg att gag gca gtt aaa gat aag aac ggc gag cag gtc act 691

Glu Leu Pro Ile Glu Ala Val Lys Asp Lys Asn Gly Glu Gln Val Thr

185 190 195

gtt tct gtc acc tac cca ggt gcg cag gaa gta aag att gca ctg tgg 739

Val Ser Val Thr Tyr Pro Gly Ala Gln Glu Val Lys Ile Ala Leu Trp

200 205 210

gta gca aac gtt ggc cgc atc cca ttg ctg ctg ctt gat acc aac atc 787

Val Ala Asn Val Gly Arg Ile Pro Leu Leu Leu Leu Asp Thr Asn Ile

215 220 225

gag gca aac cca gaa gag ctc cgc aac gtt act gac cgc ctg tac ggt 835

Glu Ala Asn Pro Glu Glu Leu Arg Asn Val Thr Asp Arg Leu Tyr Gly

230 235 240 245

ggc gac aat gag cac cgc atc aag cag gaa ctc gtt ctc ggt gtt ggt 883

Gly Asp Asn Glu His Arg Ile Lys Gln Glu Leu Val Leu Gly Val Gly

250 255 260

ggc gtc cgc gct gtc aac gca ttc tgc gaa gct cgt ggt ctg aag cgc 931

Gly Val Arg Ala Val Asn Ala Phe Cys Glu Ala Arg Gly Leu Lys Arg

265 270 275

tca tct gtt gca cac ctc aac gaa ggc cac gca ggt ttc ctg acc ctg 979

Ser Ser Val Ala His Leu Asn Glu Gly His Ala Gly Phe Leu Thr Leu

280 285 290

gag cgt atc cgc gag cgc atc gca gag ggc atg gag tac cca gca gca 1027

Glu Arg Ile Arg Glu Arg Ile Ala Glu Gly Met Glu Tyr Pro Ala Ala

295 300 305

ttc gag cag gtt cgt gcg tcc aac atc ttc acc acc cac acc cca gtc 1075

Phe Glu Gln Val Arg Ala Ser Asn Ile Phe Thr Thr His Thr Pro Val

310 315 320 325

cca gca ggc atc gac cgc ttc gac atg gag atg gtg cgt cgt tat ctc 1123

Pro Ala Gly Ile Asp Arg Phe Asp Met Glu Met Val Arg Arg Tyr Leu

330 335 340

ggt ggc ggt cag cca gaa gat cag cag ctg tgc gtt ggt gtt cca att 1171

Gly Gly Gly Gln Pro Glu Asp Gln Gln Leu Cys Val Gly Val Pro Ile

345 350 355

gag aag gca ctt gag ctt ggt caa gag tcc gat cca cac cgc ttc aac 1219

Glu Lys Ala Leu Glu Leu Gly Gln Glu Ser Asp Pro His Arg Phe Asn

360 365 370

atg gct cat atg ggc ctt cgc gcg agc caa cat gct aat ggc gtc gca 1267

Met Ala His Met Gly Leu Arg Ala Ser Gln His Ala Asn Gly Val Ala

375 380 385

aag ctt cat ggt gaa gta agc cgt gac atg ttc gcc ggc ctg tac ccc 1315

Lys Leu His Gly Glu Val Ser Arg Asp Met Phe Ala Gly Leu Tyr Pro

390 395 400 405

gga tat gag cct cgt gaa gtg ccc atc ggg cac gtc acc aac ggt gtt 1363

Gly Tyr Glu Pro Arg Glu Val Pro Ile Gly His Val Thr Asn Gly Val

410 415 420

cac ctg ccg acg tgg gtc aag cca gag atg aag gaa ctc atc gat cgc 1411

His Leu Pro Thr Trp Val Lys Pro Glu Met Lys Glu Leu Ile Asp Arg

425 430 435

gtc act ggc ggc gct gat ctt gcg gtt gct gat tct tgg tca aac cca 1459

Val Thr Gly Gly Ala Asp Leu Ala Val Ala Asp Ser Trp Ser Asn Pro

440 445 450

cag gct gtc gag tct gag aag atc tgg aag gtg cgc aac aag ttc cgt 1507

Gln Ala Val Glu Ser Glu Lys Ile Trp Lys Val Arg Asn Lys Phe Arg

455 460 465

gct gac cta gtg gag gtt gct cgc gct gca act gca aag tcc tgg tct 1555

Ala Asp Leu Val Glu Val Ala Arg Ala Ala Thr Ala Lys Ser Trp Ser

470 475 480 485

cac cgt gga cac acc gaa gca gaa ctt gcg tgg acc tcc cgc gtt ctg 1603

His Arg Gly His Thr Glu Ala Glu Leu Ala Trp Thr Ser Arg Val Leu

490 495 500

gat cca aac gtg ctg acc att ggt ttc gca cgt cgc gta tcc acc tac 1651

Asp Pro Asn Val Leu Thr Ile Gly Phe Ala Arg Arg Val Ser Thr Tyr

505 510 515

aag cgc ttg acc ttg atg ctg cgc aac cct gaa cgc ctg cgt tcc atc 1699

Lys Arg Leu Thr Leu Met Leu Arg Asn Pro Glu Arg Leu Arg Ser Ile

520 525 530

ttg ctt aat gag gaa cgc cca gtt cag ttc gtt att gct ggt aag gca 1747

Leu Leu Asn Glu Glu Arg Pro Val Gln Phe Val Ile Ala Gly Lys Ala

535 540 545

cac cca cat gac atg ggt ggc aag aag ctc atg cag gaa atc gtc cac 1795

His Pro His Asp Met Gly Gly Lys Lys Leu Met Gln Glu Ile Val His

550 555 560 565

ttc gct gat caa gct ggt gtc cgt gac cgt ttc ctc ttc ctg cct gat 1843

Phe Ala Asp Gln Ala Gly Val Arg Asp Arg Phe Leu Phe Leu Pro Asp

570 575 580

tac gac atc aac ctg gcc agc tac ctg atc tct ggt gct gac gtg tgg 1891

Tyr Asp Ile Asn Leu Ala Ser Tyr Leu Ile Ser Gly Ala Asp Val Trp

585 590 595

ctg aac aac cca gtg cgc cct cag gaa gca tcg gga acc tcc ggt atg 1939

Leu Asn Asn Pro Val Arg Pro Gln Glu Ala Ser Gly Thr Ser Gly Met

600 605 610

aag gcc gtc atg aat ggt ggc ctg acc ctg tcc atc tct gat ggt tgg 1987

Lys Ala Val Met Asn Gly Gly Leu Thr Leu Ser Ile Ser Asp Gly Trp

615 620 625

tgg gat gaa atg cct aag gag acc acc ggc tgg acc atc cca acc gtt 2035

Trp Asp Glu Met Pro Lys Glu Thr Thr Gly Trp Thr Ile Pro Thr Val

630 635 640 645

gag tcc cag gac ttg gaa tgc cgc gac cac ctg gaa tcc cag gcg ctg 2083

Glu Ser Gln Asp Leu Glu Cys Arg Asp His Leu Glu Ser Gln Ala Leu

650 655 660

tac gac ctg ctg gaa aac gaa gtt gca ccg ctg ttt tac aag cgc gac 2131

Tyr Asp Leu Leu Glu Asn Glu Val Ala Pro Leu Phe Tyr Lys Arg Asp

665 670 675

aag aac ggc atc cca cag gac tgg ctg gac ctg gtt cgc gaa tcc tgg 2179

Lys Asn Gly Ile Pro Gln Asp Trp Leu Asp Leu Val Arg Glu Ser Trp

680 685 690

acc acc ctg tca cca atg gtc acc tcc acc cgc atg gtg cgc gac tac 2227

Thr Thr Leu Ser Pro Met Val Thr Ser Thr Arg Met Val Arg Asp Tyr

695 700 705

acc acc cag tac tac cgc cca acc aaa cac cag gca gag ctc att gcg 2275

Thr Thr Gln Tyr Tyr Arg Pro Thr Lys His Gln Ala Glu Leu Ile Ala

710 715 720 725

cag cct gca gaa gca gcg gat tac gcg gca tgg ctt gag cac atc aaa 2323

Gln Pro Ala Glu Ala Ala Asp Tyr Ala Ala Trp Leu Glu His Ile Lys

730 735 740

gca gag tgg gct ggc gtc aag gtc tca gac ctg aag atc agc gag agc 2371

Ala Glu Trp Ala Gly Val Lys Val Ser Asp Leu Lys Ile Ser Glu Ser

745 750 755

gcc atc acg gcg cag gag ctt gaa gtc agc gtt cgc gtt gat tcc ggt 2419

Ala Ile Thr Ala Gln Glu Leu Glu Val Ser Val Arg Val Asp Ser Gly

760 765 770

tcg ctt aac gac gac gag ttc caa gct cag gca ctc ttt ggt gcg ctc 2467

Ser Leu Asn Asp Asp Glu Phe Gln Ala Gln Ala Leu Phe Gly Ala Leu

775 780 785

gga cac aac ggt gac atc gaa gat cca gaa atc acc gtt ttg acc cca 2515

Gly His Asn Gly Asp Ile Glu Asp Pro Glu Ile Thr Val Leu Thr Pro

790 795 800 805

cgc ggc gat ggc gcc tac gcg gca aag gtc agc act gac ctg cca ggc 2563

Arg Gly Asp Gly Ala Tyr Ala Ala Lys Val Ser Thr Asp Leu Pro Gly

810 815 820

aac tac ggc atc act gcc cgc gtt gtt cca aac aac agg atg ctg gtc 2611

Asn Tyr Gly Ile Thr Ala Arg Val Val Pro Asn Asn Arg Met Leu Val

825 830 835

agc cca gcg gaa acc cgc ctg atc acc tac ttg gag aac tagggcgaaa 2660

Ser Pro Ala Glu Thr Arg Leu Ile Thr Tyr Leu Glu Asn

840 845 850

ctagctttac caaaaggccg 2680

<210>66

<211>850

<212>PRT

<213>谷氨酸棒状杆菌

<400>66

Leu Lys Pro Val Gly Thr Val Gln Phe Lys Asn Gln Leu Pro Thr Gln

1 5 10 15

Leu Gln Pro Leu Val Lys Leu Ala His Asn Leu Arg Trp Ser Trp Arg

20 25 30

Glu Glu Ala Lys Gln Leu Phe Arg Asp Ile Asp Pro Glu Leu Trp Glu

35 40 45

Gln Leu Asp Glu Asp Pro Lys Gln Met Leu Leu Gln Ala Pro Ala Thr

50 55 60

Arg Leu Gln Phe Leu Ala Gly Asp Asp Glu Tyr Leu Gly Arg Val Asn

65 70 75 80

Ala Glu Glu Gln Asn Leu Ser Glu Tyr Leu Ser Asp Lys Leu Trp Tyr

85 90 95

Gln Asp Thr Ala Asp Ala Thr Asp Ala Val Gly Asp Pro Leu Val Ala

100 105 110

Tyr Phe Ser Met Glu Phe Gly Ile His Pro Ser Leu Pro Ile Tyr Ser

115 120 125

Gly Gly Leu Gly Val Leu Ala Gly Glu Asn Met Lys Ser Ala Ser Asp

130 135 140

Leu Gly Val Pro Leu Ile Gly Val Gly Leu Leu Tyr Thr His Gly Tyr

145 150 155 160

Phe Thr Gln Ser Leu Ser Gly Asp Gly Trp Gln Gln Glu Glu Tyr Lys

165 170 175

Tyr His Asp Pro Ala Glu Leu Pro Ile Glu Ala Val Lys Asp Lys Asn

180 185 190

Gly Glu Gln Val Thr Val Ser Val Thr Tyr Pro Gly Ala Gln Glu Val

195 200 205

Lys Ile Ala Leu Trp Val Ala Asn Val Gly Arg Ile Pro Leu Leu Leu

210 215 220

Leu Asp Thr Asn Ile Glu Ala Asn Pro Glu Glu Leu Arg Asn Val Thr

225 230 235 240

Asp Arg Leu Tyr Gly Gly Asp Asn Glu His Arg Ile Lys Gln Glu Leu

245 250 255

Val Leu Gly Val Gly Gly Val Arg Ala Val Asn Ala Phe Cys Glu Ala

260 265 270

Arg Gly Leu Lys Arg Ser Ser Val Ala His Leu Asn Glu Gly His Ala

275 280 285

Gly Phe Leu Thr Leu Glu Arg Ile Arg Glu Arg Ile Ala Glu Gly Met

290 295 300

Glu Tyr Pro Ala Ala Phe Glu Gln Val Arg Ala Ser Asn Ile Phe Thr

305 310 315 320

Thr His Thr Pro Val Pro Ala Gly Ile Asp Arg Phe Asp Met Glu Met

325 330 335

Val Arg Arg Tyr Leu Gly Gly Gly Gln Pro Glu Asp Gln Gln Leu Cys

340 345 350

Val Gly Val Pro Ile Glu Lys Ala Leu Glu Leu Gly Gln Glu Ser Asp

355 360 365

Pro His Arg Phe Asn Met Ala His Met Gly Leu Arg Ala Ser Gln His

370 375 380

Ala Asn Gly Val Ala Lys Leu His Gly Glu Val Ser Arg Asp Met Phe

385 390 395 400

Ala Gly Leu Tyr Pro Gly Tyr Glu Pro Arg Glu Val Pro Ile Gly His

405 410 415

Val Thr Asn Gly Val His Leu Pro Thr Trp Val Lys Pro Glu Met Lys

420 425 430

Glu Leu Ile Asp Arg Val Thr Gly Gly Ala Asp Leu Ala Val Ala Asp

435 440 445

Ser Trp Ser Asn Pro Gln Ala Val Glu Ser Glu Lys Ile Trp Lys Val

450 455 460

Arg Asn Lys Phe Arg Ala Asp Leu Val Glu Val Ala Arg Ala Ala Thr

465 470 475 480

Ala Lys Ser Trp Ser His Arg Gly His Thr Glu Ala Glu Leu Ala Trp

485 490 495

Thr Ser Arg Val Leu Asp Pro Asn Val Leu Thr Ile Gly Phe Ala Arg

500 505 510

Arg Val Ser Thr Tyr Lys Arg Leu Thr Leu Met Leu Arg Asn Pro Glu

515 520 525

Arg Leu Arg Ser Ile Leu Leu Asn Glu Glu Arg Pro Val Gln Phe Val

530 535 540

Ile Ala Gly Lys Ala His Pro His Asp Met Gly Gly Lys Lys Leu Met

545 550 555 560

Gln Glu Ile Val His Phe Ala Asp Gln Ala Gly Val Arg Asp Arg Phe

565 570 575

Leu Phe Leu Pro Asp Tyr Asp Ile Asn Leu Ala Ser Tyr Leu Ile Ser

580 585 590

Gly Ala Asp Val Trp Leu Asn Asn Pro Val Arg Pro Gln Glu Ala Ser

595 600 605

Gly Thr Ser Gly Met Lys Ala Val Met Asn Gly Gly Leu Thr Leu Ser

610 615 620

Ile Ser Asp Gly Trp Trp Asp Glu Met Pro Lys Glu Thr Thr Gly Trp

625 630 635 640

Thr Ile Pro Thr Val Glu Ser Gln Asp Leu Glu Cys Arg Asp His Leu

645 650 655

Glu Ser Gln Ala Leu Tyr Asp Leu Leu Glu Asn Glu Val Ala Pro Leu

660 665 670

Phe Tyr Lys Arg Asp Lys Asn Gly Ile Pro Gln Asp Trp Leu Asp Leu

675 680 685

Val Arg Glu Ser Trp Thr Thr Leu Ser Pro Met Val Thr Ser Thr Arg

690 695 700

Met Val Arg Asp Tyr Thr Thr Gln Tyr Tyr Arg Pro Thr Lys His Gln

705 710 715 720

Ala Glu Leu Ile Ala Gln Pro Ala Glu Ala Ala Asp Tyr Ala Ala Trp

725 730 735

Leu Glu His Ile Lys Ala Glu Trp Ala Gly Val Lys Val Ser Asp Leu

740 745 750

Lys Ile Ser Glu Ser Ala Ile Thr Ala Gln Glu Leu Glu Val Ser Val

755 760 765

Arg Val Asp Ser Gly Ser Leu Asn Asp Asp Glu Phe Gln Ala Gln Ala

770 775 780

Leu Phe Gly Ala Leu Gly His Asn Gly Asp Ile Glu Asp Pro Glu Ile

785 790 795 800

Thr Val Leu Thr Pro Arg Gly Asp Gly Ala Tyr Ala Ala Lys Val Ser

805 810 815

Thr Asp Leu Pro Gly Asn Tyr Gly Ile Thr Ala Arg Val Val Pro Asn

820 825 830

Asn Arg Met Leu Val Ser Pro Ala Glu Thr Arg Leu Ile Thr Tyr Leu

835 840 845

Glu Asn

850

<210>67

<211>1411

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1381)

<223>RXA02144

<400>67

agaaggacat catcgccttc atcaagtcca ccaaggagac tccatcacct ggtggttact 60

cactcggtag cttgggccca gtggctgagg gtctgttcat gtg ggt att cgg cat 115

Val Gly Ile Arg His

1 5

ctt ggt cct cgt ggc cgc cgc tat gtg gat tgg atc acg ttc atg agt 163

Leu Gly Pro Arg Gly Arg Arg Tyr Val Asp Trp Ile Thr Phe Met Ser

10 15 20

aac aac aac gac aaa cag tac aca acc caa gaa ctc aac gcg atg agc 211

Asn Asn Asn Asp Lys Gln Tyr Thr Thr Gln Glu Leu Asn Ala Met Ser

25 30 35

aat gag gat ctt gca cga ctt ggt aca gag ctg gac gac gtt acc att 259

Asn Glu Asp Leu Ala Arg Leu Gly Thr Glu Leu Asp Asp Val Thr Ile

40 45 50

gca tac cgc aag gaa cgt ttc cca atc gct aat gac cca gct gag aag 307

Ala Tyr Arg Lys Glu Arg Phe Pro Ile Ala Asn Asp Pro Ala Glu Lys

55 60 65

cgc gct gca cgt gca gtt act ttc tgg cta gtc ctc ggc atc att ggt 355

Arg Ala Ala Arg Ala Val Thr Phe Trp Leu Val Leu Gly Ile Ile Gly

70 75 80 85

gga ctt ggg ttc ctg gct acc tac att ttc tgg cct tgg gag tac aag 403

Gly Leu Gly Phe Leu Ala Thr Tyr Ile Phe Trp Pro Trp Glu Tyr Lys

90 95 100

gca cac gga gat gaa ggt ctc ctg gcg tac acc ttg tac acc cca atg 451

Ala His Gly Asp Glu Gly Leu Leu Ala Tyr Thr Leu Tyr Thr Pro Met

105 110 115

ctg ggt att act tcc ggt ctt tgc atc ctg tcc ctg gga ttt gca gtt 499

Leu Gly Ile Thr Ser Gly Leu Cys Ile Leu Ser Leu Gly Phe Ala Val

120 125 130

gtc ctt tat gtc aag aag ttc att cca gag gaa atc gca gta cag cgt 547

Val Leu Tyr Val Lys Lys Phe Ile Pro Glu Glu Ile Ala Val Gln Arg

135 140 145

cgc cac gac ggt cct tct gaa gaa gtt gac cgc cgc acc atc gtt gca 595

Arg His Asp Gly Pro Ser Glu Glu Val Asp Arg Arg Thr Ile Val Ala

150 155 160 165

ctt ctc aat gac tct tgg cag acc tct act ctt ggt cgt cgc aag ctg 643

Leu Leu Asn Asp Ser Trp Gln Thr Ser Thr Leu Gly Arg Arg Lys Leu

170 175 180

atc atg gga ctt gca ggt ggc gga gca gta ctg gcc ggc ctg acc atc 691

Ile Met Gly Leu Ala Gly Gly Gly Ala Val Leu Ala Gly Leu Thr Ile

185 190 195

atc gct cca atg ggc ggt atg atc aag aac cct tgg aat cct aag gaa 739

Ile Ala Pro Met Gly Gly Met Ile Lys Asn Pro Trp Asn Pro Lys Glu

200 205 210

ggc cca atg gac gtt cag ggt gac ggc acc ctg tgg act tcc ggt tgg 787

Gly Pro Met Asp Val Gln Gly Asp Gly Thr Leu Trp Thr Ser Gly Trp

215 220 225

act ctc gtt gag aac gac gtc aag gtt tac ctc ggc cgc gac act gca 835

Thr Leu Val Glu Asn Asp Val Lys Val Tyr Leu Gly Arg Asp Thr Ala

230 235 240 245

gca att gcg gag tcc cac acc gat gca acc ggt gag cac tgg tca acc 883

Ala Ile Ala Glu Ser His Thr Asp Ala Thr Gly Glu His Trp Ser Thr

250 255 260

act ggt gtt tcc cgc ctg gtt cgt atg cgc cca gaa gat ctg gca gca 931

Thr Gly Val Ser Arg Leu Val Arg Met Arg Pro Glu Asp Leu Ala Ala

265 270 275

gca tcc atg gaa act gtc ttc cca ctt cca gct gaa atg gtg aac gac 979

Ala Ser Met Glu Thr Val Phe Pro Leu Pro Ala Glu Met Val Asn Asp

280 285 290

ggt gct gaa tac gat cct gcg aag gac gtc tac gag cac caa atg cac 1027

Gly Ala Glu Tyr Asp Pro Ala Lys Asp Val Tyr Glu His Gln Met His

295 300 305

tcg gtg cac ggc cca cgc aac gca gtt atg ttg atc cgt ctc cgt acc 1075

Ser Val His Gly Pro Arg Asn Ala Val Met Leu Ile Arg Leu Arg Thr

310 315 320 325

gct gac gct gaa aag gtt atc gaa cgc gaa ggc cag gag tcc ttc cac 1123

Ala Asp Ala Glu Lys Val Ile Glu Arg Glu Gly Gln Glu Ser Phe His

330 335 340

tac ggt gac tac tac gct tac tcc aag att tgt aca cac att ggt tgc 1171

Tyr Gly Asp Tyr Tyr Ala Tyr Ser Lys Ile Cys Thr His Ile Gly Cys

345 350 355

cca acc tca ctg tac gag gct cag acc aat cgt att ctg tgc cca tgt 1219

Pro Thr Ser Leu Tyr Glu Ala Gln Thr Asn Arg Ile Leu Cys Pro Cys

360 365 370

cac cag tcg cag ttt gac gca ttg cac tac gga aag cca gtc ttt gga 1267

His Gln Ser Gln Phe Asp Ala Leu His Tyr Gly Lys Pro Val Phe Gly

375 380 385

cct gct gcc cgt gca ctg cca cag ctg cca att acc gtt gat gaa gag 1315

Pro Ala Ala Arg Ala Leu Pro Gln Leu Pro Ile Thr Val Asp Glu Glu

390 395 400 405

ggc tac ctc atc gcc gct ggt aac ttc att gag cca ctc ggc cct gca 1363

Gly Tyr Leu Ile Ala Ala Gly Asn Phe Ile Glu Pro Leu Gly Pro Ala

410 415 420

ttc tgg gag cgt aag tca tgagtctagc taccgtggga aacaatcttg 1411

Phe Trp Glu Arg Lys Ser

425

<210>68

<211>427

<212>PRT

<213>谷氨酸棒状杆菌

<400>68

Val Gly Ile Arg His Leu Gly Pro Arg Gly Arg Arg Tyr Val Asp Trp

1 5 10 15

Ile Thr Phe Met Ser Asn Asn Asn Asp Lys Gln Tyr Thr Thr Gln Glu

20 25 30

Leu Asn Ala Met Ser Asn Glu Asp Leu Ala Arg Leu Gly Thr Glu Leu

35 40 45

Asp Asp Val Thr Ile Ala Tyr Arg Lys Glu Arg Phe Pro Ile Ala Asn

50 55 60

Asp Pro Ala Glu Lys Arg Ala Ala Arg Ala Val Thr Phe Trp Leu Val

65 70 75 80

Leu Gly Ile Ile Gly Gly Leu Gly Phe Leu Ala Thr Tyr Ile Phe Trp

85 90 95

Pro Trp Glu Tyr Lys Ala His Gly Asp Glu Gly Leu Leu Ala Tyr Thr

100 105 110

Leu Tyr Thr Pro Met Leu Gly Ile Thr Ser Gly Leu Cys Ile Leu Ser

115 120 125

Leu Gly Phe Ala Val Val Leu Tyr Val Lys Lys Phe Ile Pro Glu Glu

130 135 140

Ile Ala Val Gln Arg Arg His Asp Gly Pro Ser Glu Glu Val Asp Arg

145 150 155 160

Arg Thr Ile Val Ala Leu Leu Asn Asp Ser Trp Gln Thr Ser Thr Leu

165 170 175

Gly Arg Arg Lys Leu Ile Met Gly Leu Ala Gly Gly Gly Ala Val Leu

180 185 190

Ala Gly Leu Thr Ile Ile Ala Pro Met Gly Gly Met Ile Lys Asn Pro

195 200 205

Trp Asn Pro Lys Glu Gly Pro Met Asp Val Gln Gly Asp Gly Thr Leu

210 215 220

Trp Thr Ser Gly Trp Thr Leu Val Glu Asn Asp Val Lys Val Tyr Leu

225 230 235 240

Gly Arg Asp Thr Ala Ala Ile Ala Glu Ser His Thr Asp Ala Thr Gly

245 250 255

Glu His Trp Ser Thr Thr Gly Val Ser Arg Leu Val Arg Met Arg Pro

260 265 270

Glu Asp Leu Ala Ala Ala Ser Met Glu Thr Val Phe Pro Leu Pro Ala

275 280 285

Glu Met Val Asn Asp Gly Ala Glu Tyr Asp Pro Ala Lys Asp Val Tyr

290 295 300

Glu His Gln Met His Ser Val His Gly Pro Arg Asn Ala Val Met Leu

305 310 315 320

Ile Arg Leu Arg Thr Ala Asp Ala Glu Lys Val Ile Glu Arg Glu Gly

325 330 335

Gln Glu Ser Phe His Tyr Gly Asp Tyr Tyr Ala Tyr Ser Lys Ile Cys

340 345 350

Thr His Ile Gly Cys Pro Thr Ser Leu Tyr Glu Ala Gln Thr Asn Arg

355 360 365

Ile Leu Cys Pro Cys His Gln Ser Gln Phe Asp Ala Leu His Tyr Gly

370 375 380

Lys Pro Val Phe Gly Pro Ala Ala Arg Ala Leu Pro Gln Leu Pro Ile

385 390 395 400

Thr Val Asp Glu Glu Gly Tyr Leu Ile Ala Ala Gly Asn Phe Ile Glu

405 410 415

Pro Leu Gly Pro Ala Phe Trp Glu Arg Lys Ser

420 425

<210>69

<211>1099

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1069)

<223>RXA02149

<400>69

ttgcagagtg aaccacgatg atggttggac agctgttgat agctaatctt tgaaagatta 60

aattcaccta aatcctgtgt agaacgcgag gggcactctt atg cca caa aaa ccg 115

Met Pro Gln Lys Pro

1 5

gcc agt ttc gcg gtg ggc ttt gac atc ggc ggc acc aac atg cga gcc 163

Ala Ser Phe Ala Val Gly Phe Asp Ile Gly Gly Thr Asn Met Arg Ala

10 15 20

ggg ctt gtc gac gaa tcc ggg cgc atc gtg acc agt ttg tcg gcg ccg 211

Gly Leu Val Asp Glu Ser Gly Arg Ile Val Thr Ser Leu Ser Ala Pro

25 30 35

tcg ccg cgc acg acg cag gca atg gaa cag ggg att ttt gat cta gtc 259

Ser Pro Arg Thr Thr Gln Ala Met Glu Gln Gly Ile Phe Asp Leu Val

40 45 50

gaa cag ctc aag gcc gaa tac ccg gtt ggt gct gtg gga ctt gcc gtc 307

Glu Gln Leu Lys Ala Glu Tyr Pro Val Gly Ala Val Gly Leu Ala Val

55 60 65

gcg gga ttt ttg gat cct gag tgc gag gtt gtt cga ttt gcc ccg cac 355

Ala Gly Phe Leu Asp Pro Glu Cys Glu Val Val Arg Phe Ala Pro His

70 75 80 85

ctt cct tgg cgc gat gag cca gtg cgt gaa aag ttg gaa aac ctt ttg 403

Leu Pro Trp Arg Asp Glu Pro Val Arg Glu Lys Leu Glu Asn Leu Leu

90 95 100

ggc ctg cct gtt cgt ttg gaa cat gat gcc aac tca gca gcg tgg ggt 451

Gly Leu Pro Val Arg Leu Glu His Asp Ala Asn Ser Ala Ala Trp Gly

105 110 115

gag cat cgt ttt ggt gca gct caa ggc gct gac aac tgg gtt ttg ttg 499

Glu His Arg Phe Gly Ala Ala Gln Gly Ala Asp Asn Trp Val Leu Leu

120 125 130

gca ctc ggc act gga att ggt gca gcg ctg att gaa aaa ggc gaa att 547

Ala Leu Gly Thr Gly Ile Gly Ala Ala Leu Ile Glu Lys Gly Glu Ile

135 140 145

tac cgt ggt gca tat ggc acg gca cca gaa ttt ggt cat ttg cgt gtt 595

Tyr Arg Gly Ala Tyr Gly Thr Ala Pro Glu Phe Gly His Leu Arg Val

150 155 160 165

gtt cgt ggc gga cgc gca tgt gcg tgt ggc aaa gaa ggc tgc ctg gag 643

Val Arg Gly Gly Arg Ala Cys Ala Cys Gly Lys Glu Gly Cys Leu Glu

170 175 180

cgt tac tgt tcc ggt act gcc ttg gtt tac act gcg cgt gaa ttg gct 691

Arg Tyr Cys Ser Gly Thr Ala Leu Val Tyr Thr Ala Arg Glu Leu Ala

185 190 195

tcg cat ggc tca ttc cgc aac agc ggg ctg ttt gac aag atc aaa gcc 739

Ser His Gly Ser Phe Arg Asn Ser Gly Leu Phe Asp Lys Ile Lys Ala

200 205 210

gat ccg aat tcc atc aat gga aaa acg atc act gcg gca gcg cgc caa 787

Asp Pro Asn Ser Ile Asn Gly Lys Thr Ile Thr Ala Ala Ala Arg Gln

215 220 225

gaa gac cca ctt gct ctc gcc gtt ctg gaa gat ttc agc gag tgg ctg 835

Glu Asp Pro Leu Ala Leu Ala Val Leu Glu Asp Phe Ser Glu Trp Leu

230 235 240 245

ggc gaa act ttg gcg atc att gct gat gtc ctt gac cca ggc atg atc 883

Gly Glu Thr Leu Ala Ile Ile Ala Asp Val Leu Asp Pro Gly Met Ile

250 255 260

atc att ggt ggc gga ctg tcc aat gct gcc gac ctt tat ttg gat cgc 931

Ile Ile Gly Gly Gly Leu Ser Asn Ala Ala Asp Leu Tyr Leu Asp Arg

265 270 275

tcg gtc aac cac tat tcc acc cgc atc gtc ggc gca gga tat cgc cct 979

Ser Val Asn His Tyr Ser Thr Arg Ile Val Gly Ala Gly Tyr Arg Pro

280 285 290

ttg gca cgc gtt gcc aca gct cag ttg ggt gcg gat gct ggc atg atc 1027

Leu Ala Arg Val Ala Thr Ala Gln Leu Gly Ala Asp Ala Gly Met Ile

295 300 305

ggt gtc gct gat cta gct cga cgc tct gta gtg gaa gcc aac 1069

Gly Val Ala Asp Leu Ala Arg Arg Ser Val Val Glu Ala Asn

310 315 320

taggtgtttt tcggtgggct gcgatgacgc 1099

<210>70

<211>323

<212>PRT

<213>谷氨酸棒状杆菌

<400>70

Met Pro Gln Lys Pro Ala Ser Phe Ala Val Gly Phe Asp Ile Gly Gly

1 5 10 15

Thr Asn Met Arg Ala Gly Leu Val Asp Glu Ser Gly Arg Ile Val Thr

20 25 30

Ser Leu Ser Ala Pro Ser Pro Arg Thr Thr Gln Ala Met Glu Gln Gly

35 40 45

Ile Phe Asp Leu Val Glu Gln Leu Lys Ala Glu Tyr Pro Val Gly Ala

50 55 60

Val Gly Leu Ala Val Ala Gly Phe Leu Asp Pro Glu Cys Glu Val Val

65 70 75 80

Arg Phe Ala Pro His Leu Pro Trp Arg Asp Glu Pro Val Arg Glu Lys

85 90 95

Leu Glu Asn Leu Leu Gly Leu Pro Val Arg Leu Glu His Asp Ala Asn

100 105 110

Ser Ala Ala Trp Gly Glu His Arg Phe Gly Ala Ala Gln Gly Ala Asp

115 120 125

Asn Trp Val Leu Leu Ala Leu Gly Thr Gly Ile Gly Ala Ala Leu Ile

130 135 140

Glu Lys Gly Glu Ile Tyr Arg Gly Ala Tyr Gly Thr Ala Pro Glu Phe

145 150 155 160

Gly His Leu Arg Val Val Arg Gly Gly Arg Ala Cys Ala Cys Gly Lys

165 170 175

Glu Gly Cys Leu Glu Arg Tyr Cys Ser Gly Thr Ala Leu Val Tyr Thr

180 185 190

Ala Arg Glu Leu Ala Ser His Gly Ser Phe Arg Asn Ser Gly Leu Phe

195 200 205

Asp Lys Ile Lys Ala Asp Pro Asn Ser Ile Asn Gly Lys Thr Ile Thr

210 215 220

Ala Ala Ala Arg Gln Glu Asp Pro Leu Ala Leu Ala Val Leu Glu Asp

225 230 235 240

Phe Ser Glu Trp Leu Gly Glu Thr Leu Ala Ile Ile Ala Asp Val Leu

245 250 255

Asp Pro Gly Met Ile Ile Ile Gly Gly Gly Leu Ser Asn Ala Ala Asp

260 265 270

Leu Tyr Leu Asp Arg Ser Val Asn His Tyr Ser Thr Arg Ile Val Gly

275 280 285

Ala Gly Tyr Arg Pro Leu Ala Arg Val Ala Thr Ala Gln Leu Gly Ala

290 295 300

Asp Ala Gly Met Ile Gly Val Ala Asp Leu Ala Arg Arg Ser Val Val

305 310 315 320

Glu Ala Asn

<210>71

<211>883

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(853)

<223>RXA02196

<400>71

gcgtggagtt gtccgaggaa ctccagctgc acccagagca gtccacagac gcgtttgtgc 60

tctaccaccc agaggcaaag tactttaacg tctaacacct ttg aga ggg aaa act 115

Leu Arg Gly Lys Thr

1 5

ttc ccg cac att gca gat cgt gcc act tta act aag gtt gac ggc atg 163

Phe Pro His Ile Ala Asp Arg Ala Thr Leu Thr Lys Val Asp Gly Met

10 15 20

att aag gcg att ttc tgg gac atg gac ggc acg atg gtg gac tct gag 211

Ile Lys Ala Ile Phe Trp Asp Met Asp Gly Thr Met Val Asp Ser Glu

25 30 35

cca cag tgg ggc att gct acc tac gag ctc agc gaa gcc atg ggc cgc 259

Pro Gln Trp Gly Ile Ala Thr Tyr Glu Leu Ser Glu Ala Met Gly Arg

40 45 50

cgc ctc acc ccg gag ctc cgg gaa ctc acc gtc ggc tcg agc ctg ccg 307

Arg Leu Thr Pro Glu Leu Arg Glu Leu Thr Val Gly Ser Ser Leu Pro

55 60 65

cgc acc atg cgc tta tgc gca gag cac gca ggc att aca ttg agc gac 355

Arg Thr Met Arg Leu Cys Ala Glu His Ala Gly Ile Thr Leu Ser Asp

70 75 80 85

gcg gac tac gag cgc tac cgg gct ggc atg ttc gcc cgg gtc cat gag 403

Ala Asp Tyr Glu Arg Tyr Arg Ala Gly Met Phe Ala Arg Val His Glu

90 95 100

ctt ttc gac gaa tcc ctc gtc cca aat cca ggc gtc acc gaa ctc ctg 451

Leu Phe Asp Glu Ser Leu Val Pro Asn Pro Gly Val Thr Glu Leu Leu

105 110 115

aca gag ttg aag gcc ctc gag atc ccc atg ttg gtc acc acc aac aca 499

Thr Glu Leu Lys Ala Leu Glu Ile Pro Met Leu Val Thr Thr Asn Thr

120 125 130

gag cgc gat ctc gcg acc cgt tca gtc gca gcc gtg gga aat gag ttc 547

Glu Arg Asp Leu Ala Thr Arg Ser Val Ala Ala Val Gly Asn Glu Phe

135 140 145

ttc atc ggt tct atc gct ggt gat gaa gtc cca aca gca aag cca gcc 595

Phe Ile Gly Ser Ile Ala Gly Asp Glu Val Pro Thr Ala Lys Pro Ala

150 155 160 165

ccc gac atg tac ctc gaa gca gca cga cgt gtg ggc ttt gac cca tca 643

Pro Asp Met Tyr Leu Glu Ala Ala Arg Arg Val Gly Phe Asp Pro Ser

170 175 180

gag tgc ctc gtg ttc gaa gat tcc tac aac ggc atg ctg ggc gct gtt 691

Glu Cys Leu Val Phe Glu Asp Ser Tyr Asn Gly Met Leu Gly Ala Val

185 190 195

act gca ggt tgc cgc gtc att ggt ctg cac cca gaa gaa gtc caa gcg 739

Thr Ala Gly Cys Arg Val Ile Gly Leu His Pro Glu Glu Val Gln Ala

200 205 210

cca gaa ggt gta gtg cct ttg cgt tcc ctc cac ggt aaa aac tct ttc 787

Pro Glu Gly Val Val Pro Leu Arg Ser Leu His Gly Lys Asn Ser Phe

215 220 225

gaa ggt gtc acc gct gag atg gtc act gcc tgg tac cac cag atc gag 835

Glu Gly Val Thr Ala Glu Met Val Thr Ala Trp Tyr His Gln Ile Glu

230 235 240 245

ccg gca ggt gtc gca aaa taaaaccagg tgggggagtg aaattattcg 883

Pro Ala Gly Val Ala Lys

250

<210>72

<211>251

<212>PRT

<213>谷氨酸棒状杆菌

<400>72

Leu Arg Gly Lys Thr Phe Pro His Ile Ala Asp Arg Ala Thr Leu Thr

1 5 10 15

Lys Val Asp Gly Met Ile Lys Ala Ile Phe Trp Asp Met Asp Gly Thr

20 25 30

Met Val Asp Ser Glu Pro Gln Trp Gly Ile Ala Thr Tyr Glu Leu Ser

35 40 45

Glu Ala Met Gly Arg Arg Leu Thr Pro Glu Leu Arg Glu Leu Thr Val

50 55 60

Gly Ser Ser Leu Pro Arg Thr Met Arg Leu Cys Ala Glu His Ala Gly

65 70 75 80

Ile Thr Leu Ser Asp Ala Asp Tyr Glu Arg Tyr Arg Ala Gly Met Phe

85 90 95

Ala Arg Val His Glu Leu Phe Asp Glu Ser Leu Val Pro Asn Pro Gly

100 105 110

Val Thr Glu Leu Leu Thr Glu Leu Lys Ala Leu Glu Ile Pro Met Leu

115 120 125

Val Thr Thr Asn Thr Glu Arg Asp Leu Ala Thr Arg Ser Val Ala Ala

130 135 140

Val Gly Asn Glu Phe Phe Ile Gly Ser Ile Ala Gly Asp Glu Val Pro

145 150 155 160

Thr Ala Lys Pro Ala Pro Asp Met Tyr Leu Glu Ala Ala Arg Arg Val

165 170 175

Gly Phe Asp Pro Ser Glu Cys Leu Val Phe Glu Asp Ser Tyr Asn Gly

180 185 190

Met Leu Gly Ala Val Thr Ala Gly Cys Arg Val Ile Gly Leu His Pro

195 200 205

Glu Glu Val Gln Ala Pro Glu Gly Val Val Pro Leu Arg Ser Leu His

210 215 220

Gly Lys Asn Ser Phe Glu Gly Val Thr Ala Glu Met Val Thr Ala Trp

225 230 235 240

Tyr His Gln Ile Glu Pro Ala Gly Val Ala Lys

245 250

<210>73

<211>1198

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1168)

<223>RXA02206

<400>73

gccggcatcg gcaccccaga acagctcaga gatgattcgg aggtgtccgc tggagctgat 60

cggcaggaac tctgtgaggc cttgaacgat ggagagcaca atg gtt tgt acc cag 115

Met Val Cys Thr Gln

1 5

cca ata ttt tca gtt gcg gca gga tct gct gct gcg gct agg agg gtt 163

Pro Ile Phe Ser Val Ala Ala Gly Ser Ala Ala Ala Ala Arg Arg Val

10 15 20

atc tct tca ttc acc cga tct acc gta cta cct tat gac ctc agt agt 211

Ile Ser Ser Phe Thr Arg Ser Thr Val Leu Pro Tyr Asp Leu Ser Ser

25 30 35

gtg gtg ggc gtg aaa cag cga atg gtc ggt tca agt ggt ttg cgg gta 259

Val Val Gly Val Lys Gln Arg Met Val Gly Ser Ser Gly Leu Arg Val

40 45 50

tcc agg ctc ggt ttg ggc acc tca aca tgg ggc tcg ggc acc gag ctg 307

Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly Ser Gly Thr Glu Leu

55 60 65

gct gag gca ggc gat atc ttt aag gcg ttc atc aat tct ggt ggc acg 355

Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile Asn Ser Gly Gly Thr

70 75 80 85

ctt atc gac gtc tcc ccc aac tac acc acc ggc gtc gcg gaa gaa atg 403

Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly Val Ala Glu Glu Met

90 95 100

ctc ggc acg atg ttg gat gcg gaa gtc tct cgt tcg gct gtc gtc att 451

Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg Ser Ala Val Val Ile

105 110 115

tcc tcc agc gca ggt gtc aac ccc gct ctg ccg ctc ggc cga cgt gtg 499

Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro Leu Gly Arg Arg Val

120 125 130

gat tgc tcc cgc cgc aat ttg att gcc caa tta gat gtc acc ctg cgg 547

Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu Asp Val Thr Leu Arg

135 140 145

gca tta aac act gac tat ttg gat ttg tgg tct gtg ggc tat tgg gat 595

Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser Val Gly Tyr Trp Asp

150 155 160 165

gag ggc acc cca ccg cat gag gtg gcc gat act ttg gat tac gcc gtg 643

Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr Leu Asp Tyr Ala Val

170 175 180

cgc acc ggc cga gtc cga tat gcc ggt gtc cga gga tat tcc ggt tgg 691

Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg Gly Tyr Ser Gly Trp

185 190 195

cag tta gcg gtc acc cac gct gca tcc aat cat gca gcg gcc tcc gcc 739

Gln Leu Ala Val Thr His Ala Ala Ser Asn His Ala Ala Ala Ser Ala

200 205 210

cgc ccc gtg gtc gtt gca caa aat gaa tac agc ctg ctg gaa cgc cgc 787

Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser Leu Leu Glu Arg Arg

215 220 225

gca gaa caa gaa ctc ctc cct gcc acc caa cac cta ggt gtc gga ttc 835

Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His Leu Gly Val Gly Phe

230 235 240 245

ttt gct ggc gct ccg ctg ggg caa ggc gtg ctg act gct aaa tac cgc 883

Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu Thr Ala Lys Tyr Arg

250 255 260

tcc gaa att ccc cat gat tcc aga gct gca tcc aca gga cgc gac gca 931

Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser Thr Gly Arg Asp Ala

265 270 275

gaa gtc caa agc tac cta gat aat cga ggc cgc atc att gtc gat gct 979

Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg Ile Ile Val Asp Ala

280 285 290

ctt gat act gca gcc aaa gga tta ggc att agc ccc gct gtc aca gcc 1027

Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser Pro Ala Val Thr Ala

295 300 305

acc acc tgg gtg cgt gat cgt ccc gga gtg aca gct gtc atc gtg ggc 1075

Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr Ala Val Ile Val Gly

310 315 320 325

gct cgc aca cat gaa cag ctg tca cat ctt ctc aag gcg gaa tcg gtg 1123

Ala Arg Thr His Glu Gln Leu Ser His Leu Leu Lys Ala Glu Ser Val

330 335 340

act ttg cca aca cca atc aca caa gcc ctt gat gat gtc tcc ctg 1168

Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp Asp Val Ser Leu

345 350 355

tgacttggtc caattacatt cactggtaat 1198

<210>74

<211>356

<212>PRT

<213>谷氨酸棒状杆菌

<400>74

Met Val Cys Thr Gln Pro Ile Phe Ser Val Ala Ala Gly Ser Ala Ala

1 5 10 15

Ala Ala Arg Arg Val Ile Ser Ser Phe Thr Arg Ser Thr Val Leu Pro

20 25 30

Tyr Asp Leu Ser Ser Val Val Gly Val Lys Gln Arg Met Val Gly Ser

35 40 45

Ser Gly Leu Arg Val Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly

50 55 60

Ser Gly Thr Glu Leu Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile

65 70 75 80

Asn Ser Gly Gly Thr Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly

85 90 95

Val Ala Glu Glu Met Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg

100 105 110

Ser Ala Val Val Ile Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro

115 120 125

Leu Gly Arg Arg Val Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu

130 135 140

Asp Val Thr Leu Arg Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser

145 150 155 160

Val Gly Tyr Trp Asp Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr

165 170 175

Leu Asp Tyr Ala Val Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg

180 185 190

Gly Tyr Ser Gly Trp Gln Leu Ala Val Thr His Ala Ala Ser Asn His

195 200 205

Ala Ala Ala Ser Ala Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser

210 215 220

Leu Leu Glu Arg Arg Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His

225 230 235 240

Leu Gly Val Gly Phe Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu

245 250 255

Thr Ala Lys Tyr Arg Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser

260 265 270

Thr Gly Arg Asp Ala Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg

275 280 285

Ile Ile Val Asp Ala Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser

290 295 300

Pro Ala Val Thr Ala Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr

305 310 315 320

Ala Val Ile Val Gly Ala Arg Thr His Glu Gln Leu Ser His Leu Leu

325 330 335

Lys Ala Glu Ser Val Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp

340 345 350

Asp Val Ser Leu

355

<210>75

<211>1474

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1444)

<223>RXA02399

<400>75

ttgacgcacc aatgcccgat ggagcaatgt gtgaaccacg ccaccacgca aaccgatgca 60

catcacgtcg aaacagtgac agtgcattag ctcatacttt gtg gtc ggc acc gcc 115

Val Val Gly Thr Ala

1 5

cat tgc gaa tca gca ctt aag gaa gtg act ttg atg tca aac gtt gga 163

His Cys Glu Ser Ala Leu Lys Glu Val Thr Leu Met Ser Asn Val Gly

10 15 20

aag cca cgt acc gca cag gaa atc cag cag gat tgg gac acc aac cct 211

Lys Pro Arg Thr Ala Gln Glu Ile Gln Gln Asp Trp Asp Thr Asn Pro

25 30 35

cgt tgg aac ggc atc acc cgc gac tac acc gca gac cag gta gct gat 259

Arg Trp Asn Gly Ile Thr Arg Asp Tyr Thr Ala Asp Gln Val Ala Asp

40 45 50

ctg cag ggt tcc gtc atc gag gag cac act ctt gct cgc cgc ggc tca 307

Leu Gln Gly Ser Val Ile Glu Glu His Thr Leu Ala Arg Arg Gly Ser

55 60 65

gag atc ctc tgg gac gca gtc acc cag gaa ggt gac gga tac atc aac 355

Glu Ile Leu Trp Asp Ala Val Thr Gln Glu Gly Asp Gly Tyr Ile Asn

70 75 80 85

gcg ctt ggc gca ctc acc ggt aac cag gct gtt cag cag gtt cgt gca 403

Ala Leu Gly Ala Leu Thr Gly Asn Gln Ala Val Gln Gln Val Arg Ala

90 95 100

ggc ctg aag gct gtc tac ctg tcc ggt tgg cag gtc gca ggt gac gcc 451

Gly Leu Lys Ala Val Tyr Leu Ser Gly Trp Gln Val Ala Gly Asp Ala

105 110 115

aac ctc tcc ggc cac acc tac cct gac cag tcc ctc tac cca gcg aac 499

Asn Leu Ser Gly His Thr Tyr Pro Asp Gln Ser Leu Tyr Pro Ala Asn

120 125 130

tcc gtt cca agc gtc gtt cgt cgc atc aac aac gca ctg ctg cgt tcc 547

Ser Val Pro Ser Val Val Arg Arg Ile Asn Asn Ala Leu Leu Arg Ser

135 140 145

gat gaa atc gca cgc acc gaa ggc gac acc tcc gtt gac aac tgg gtt 595

Asp Glu Ile Ala Arg Thr Glu Gly Asp Thr Ser Val Asp Asn Trp Val

150 155 160 165

gtc cca atc gtc gcg gac ggc gaa gct ggc ttc ggt gga gca ctc aac 643

Val Pro Ile Val Ala Asp Gly Glu Ala Gly Phe Gly Gly Ala Leu Asn

170 175 180

gtc tac gaa ctc cag aag gca atg atc gca gct ggc gct gca ggc acc 691

Val Tyr Glu Leu Gln Lys Ala Met Ile Ala Ala Gly Ala Ala Gly Thr

185 190 195

cac tgg gaa gac cag ctc gct tct gaa aag aag tgt ggc cac ctc ggc 739

His Trp Glu Asp Gln Leu Ala Ser Glu Lys Lys Cys Gly His Leu Gly

200 205 210

ggc aag gtt ctg atc cca acc cag cag cac atc cgc acc ctg aac tct 787

Gly Lys Val Leu Ile Pro Thr Gln Gln His Ile Arg Thr Leu Asn Ser

215 220 225

gcc cgc ctt gca gca gac gtt gca aac acc cca act gtt gtt atc gca 835

Ala Arg Leu Ala Ala Asp Val Ala Asn Thr Pro Thr Val Val Ile Ala

230 235 240 245

cgt acc gac gct gag gca gca acc ctg atc acc tct gac gtt gat gag 883

Arg Thr Asp Ala Glu Ala Ala Thr Leu Ile Thr Ser Asp Val Asp Glu

250 255 260

cgc gac caa cca ttc atc acc ggt gag cgc acc gca gaa ggc tac tac 931

Arg Asp Gln Pro Phe Ile Thr Gly Glu Arg Thr Ala Glu Gly Tyr Tyr

265 270 275

cac gtc aag aat ggt ctc gag cca tgt atc gca cgt gca aag tcc tac 979

His Val Lys Asn Gly Leu Glu Pro Cys Ile Ala Arg Ala Lys Ser Tyr

280 285 290

gca cca tac gca gat atg atc tgg atg gag acc ggc acc cct gac ctg 1027

Ala Pro Tyr Ala Asp Met Ile Trp Met Glu Thr Gly Thr Pro Asp Leu

295 300 305

gag ctc gct aag aag ttc gct gaa ggc gtt cgc tct gag ttc cca gac 1075

Glu Leu Ala Lys Lys Phe Ala Glu Gly Val Arg Ser Glu Phe Pro Asp

310 315 320 325

cag ctg ctg tcc tac aac tgc tcc cca tcc ttc aac tgg tct gca cac 1123

Gln Leu Leu Ser Tyr Asn Cys Ser Pro Ser Phe Asn Trp Ser Ala His

330 335 340

ctc gag gca gat gag atc gct aag ttc cag aag gaa ctc ggc gca atg 1171

Leu Glu Ala Asp Glu Ile Ala Lys Phe Gln Lys Glu Leu Gly Ala Met

345 350 355

ggc ttc aag ttc cag ttc atc acc ctc gca ggc ttc cac tcc ctc aac 1219

Gly Phe Lys Phe Gln Phe Ile Thr Leu Ala Gly Phe His Ser Leu Asn

360 365 370

tac ggc atg ttc gac ctg gct tac gga tac gct cgc gaa ggc atg acc 1267

Tyr Gly Met Phe Asp Leu Ala Tyr Gly Tyr Ala Arg Glu Gly Met Thr

375 380 385

tcc ttc gtt gac ctg cag aac cgt gag ttc aag gca gct gaa gag cgt 1315

Ser Phe Val Asp Leu Gln Asn Arg Glu Phe Lys Ala Ala Glu Glu Arg

390 395 400 405

ggc ttc acc gct gtt aag cac cag cgt gag gtt ggc gca ggc tac ttc 1363

Gly Phe Thr Ala Val Lys His Gln Arg Glu Val Gly Ala Gly Tyr Phe

410 415 420

gac cag atc gca acc acc gtt gac ccg aac tct tct acc acc gct ttg 1411

Asp Gln Ile Ala Thr Thr Val Asp Pro Asn Ser Ser Thr Thr Ala Leu

425 430 435

aag ggt tcc act gaa gaa ggc cag ttc cac aac taggacctac aggttctgac 1464

Lys Gly Ser Thr Glu Glu Gly Gln Phe His Asn

440 445

aatttaaatc 1474

<210>76

<211>448

<212>PRT

<213>谷氨酸棒状杆菌

<400>76

Val Val Gly Thr Ala His Cys Glu Ser Ala Leu Lys Glu Val Thr Leu

1 5 10 15

Met Ser Asn Val Gly Lys Pro Arg Thr Ala Gln Glu Ile Gln Gln Asp

20 25 30

Trp Asp Thr Asn Pro Arg Trp Asn Gly Ile Thr Arg Asp Tyr Thr Ala

35 40 45

Asp Gln Val Ala Asp Leu Gln Gly Ser Val Ile Glu Glu His Thr Leu

50 55 60

Ala Arg Arg Gly Ser Glu Ile Leu Trp Asp Ala Val Thr Gln Glu Gly

65 70 75 80

Asp Gly Tyr Ile Asn Ala Leu Gly Ala Leu Thr Gly Asn Gln Ala Val

85 90 95

Gln Gln Val Arg Ala Gly Leu Lys Ala Val Tyr Leu Ser Gly Trp Gln

100 105 110

Val Ala Gly Asp Ala Asn Leu Ser Gly His Thr Tyr Pro Asp Gln Ser

115 120 125

Leu Tyr Pro Ala Asn Ser Val Pro Ser Val Val Arg Arg Ile Asn Asn

130 135 140

Ala Leu Leu Arg Ser Asp Glu Ile Ala Arg Thr Glu Gly Asp Thr Ser

145 150 155 160

Val Asp Asn Trp Val Val Pro Ile Val Ala Asp Gly Glu Ala Gly Phe

165 170 175

Gly Gly Ala Leu Asn Val Tyr Glu Leu Gln Lys Ala Met Ile Ala Ala

180 185 190

Gly Ala Ala Gly Thr His Trp Glu Asp Gln Leu Ala Ser Glu Lys Lys

195 200 205

Cys Gly His Leu Gly Gly Lys Val Leu Ile Pro Thr Gln Gln His Ile

210 215 220

Arg Thr Leu Asn Ser Ala Arg Leu Ala Ala Asp Val Ala Asn Thr Pro

225 230 235 240

Thr Val Val Ile Ala Arg Thr Asp Ala Glu Ala Ala Thr Leu Ile Thr

245 250 255

Ser Asp Val Asp Glu Arg Asp Gln Pro Phe Ile Thr Gly Glu Arg Thr

260 265 270

Ala Glu Gly Tyr Tyr His Val Lys Asn Gly Leu Glu Pro Cys Ile Ala

275 280 285

Arg Ala Lys Ser Tyr Ala Pro Tyr Ala Asp Met Ile Trp Met Glu Thr

290 295 300

Gly Thr Pro Asp Leu Glu Leu Ala Lys Lys Phe Ala Glu Gly Val Arg

305 310 315 320

Ser Glu Phe Pro Asp Gln Leu Leu Ser Tyr Asn Cys Ser Pro Ser Phe

325 330 335

Asn Trp Ser Ala His Leu Glu Ala Asp Glu Ile Ala Lys Phe Gln Lys

340 345 350

Glu Leu Gly Ala Met Gly Phe Lys Phe Gln Phe Ile Thr Leu Ala Gly

355 360 365

Phe His Ser Leu Asn Tyr Gly Met Phe Asp Leu Ala Tyr Gly Tyr Ala

370 375 380

Arg Glu Gly Met Thr Ser Phe Val Asp Leu Gln Asn Arg Glu Phe Lys

385 390 395 400

Ala Ala Glu Glu Arg Gly Phe Thr Ala Val Lys His Gln Arg Glu Val

405 410 415

Gly Ala Gly Tyr Phe Asp Gln Ile Ala Thr Thr Val Asp Pro Asn Ser

420 425 430

Ser Thr Thr Ala Leu Lys Gly Ser Thr Glu Glu Gly Gln Phe His Asn

435 440 445

<210>77

<21l>2347

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2317)

<223>RXA02404

<400>77

gttttcacaa ccgttaacgg cgtagccaaa caagaaggat tcgcattctt ctggtttagg 60

cacaggtcat ctaaaaccca tgctttaaaa ggagccttca atg act gaa cag gaa 115

Met Thr Glu Gln Glu

1 5

ctg ttg tct gct cag act gcc gac aac gct gga act gac agc acc gaa 163

Leu Leu Ser Ala Gln Thr Ala Asp Asn Ala Gly Thr Asp Ser Thr Glu

10 15 20

cgc gtt gac gcg ggc gga atg cag gtt gca aaa gtt ctc tac gac ttt 211

Arg Val Asp Ala Gly Gly Met Gln Val Ala Lys Val Leu Tyr Asp Phe

25 30 35

gta acc gaa gcg gta ctc cct cgc gtg ggt gtg gat gcg gaa aag ttc 259

Val Thr Glu Ala Val Leu Pro Arg Val Gly Val Asp Ala Glu Lys Phe

40 45 50

tgg tcc gga ttc gcc gcc atc gcc cgg gac ctc acc cca cgc aac cgc 307

Trp Ser Gly Phe Ala Ala Ile Ala Arg Asp Leu Thr Pro Arg Asn Arg

55 60 65

gag ctg ctt gct cgc cgc gat gaa ctg cag atg ctt atc gac gac tac 355

Glu Leu Leu Ala Arg Arg Asp Glu Leu Gln Met Leu Ile Asp Asp Tyr

70 75 80 85

cac cgc aac aac tcc ggc acc atc gac caa gag gcg tac gag gat ttc 403

His Arg Asn Asn Ser Gly Thr Ile Asp Gln Glu Ala Tyr Glu Asp Phe

90 95 100

ctc aaa gaa atc gga tac ttg gtt gag gag cca gaa gct gca gaa atc 451

Leu Lys Glu Ile Gly Tyr Leu Val Glu Glu Pro Glu Ala Ala Glu Ile

105 110 115

cgt acc caa aac gtc gat acg gaa atc tcc agc acc gca gga cct cag 499

Arg Thr Gln Asn Val Asp Thr Glu Ile Ser Ser Thr Ala Gly Pro Gln

120 125 130

ctg gtt gtt cca att ctg aac gca cgc ttc gcg ctg aac gct gcc aat 547

Leu Val Val Pro Ile Leu Asn Ala Arg Phe Ala Leu Asn Ala Ala Asn

135 140 145

gct cgc tgg ggt tcc ctc tac gat gcg ttg tac ggc acc aac gcc atc 595

Ala Arg Trp Gly Ser Leu Tyr Asp Ala Leu Tyr Gly Thr Asn Ala Ile

150 155 160 165

cca gaa act gat ggc gct gaa aag ggc aag gag tac aac ccg gtc cgc 643

Pro Glu Thr Asp Gly Ala Glu Lys Gly Lys Glu Tyr Asn Pro Val Arg

170 175 180

ggc cag aag gtc atc gag tgg ggt cgt gaa ttc ctc gac agc gtt gtc 691

Gly Gln Lys Val Ile Glu Trp Gly Arg Glu Phe Leu Asp Ser Val Val

185 190 195

cca ctg gac ggt gct tcg cat gcc gat gtt gag aag tac aac atc acc 739

Pro Leu Asp Gly Ala Ser His Ala Asp Val Glu Lys Tyr Asn Ile Thr

200 205 210

gat gga aag ctt gca gcc cac att gga gat agc gtc tac cga ctg aaa 787

Asp Gly Lys Leu Ala Ala His Ile Gly Asp Ser Val Tyr Arg Leu Lys

215 220 225

aac cgt gaa tcc tac cgt ggc ttc acc ggc aac ttc ctt gat cca gaa 835

Asn Arg Glu Ser Tyr Arg Gly Phe Thr Gly Asn Phe Leu Asp Pro Glu

230 235 240 245

gca atc ctg ctg gaa acc aac ggc ctg cac atc gag ctg cag atc gat 883

Ala Ile Leu Leu Glu Thr Asn Gly Leu His Ile Glu Leu Gln Ile Asp

250 255 260

cct gtc cac cca atc ggc aag gca gac aag act ggt ctc aaa gac atc 931

Pro Val His Pro Ile Gly Lys Ala Asp Lys Thr Gly Leu Lys Asp Ile

265 270 275

gtt ttg gaa tct gcg atc acc acg atc atg gac ttc gaa gac tcc gtt 979

Val Leu Glu Ser Ala Ile Thr Thr Ile Met Asp Phe Glu Asp Ser Val

280 285 290

gca gct gtt gat gct gaa gac aag acc tta ggt tac tct aac tgg ttc 1027

Ala Ala Val Asp Ala Glu Asp Lys Thr Leu Gly Tyr Ser Asn Trp Phe

295 300 305

gga ctc aac acc ggc gaa ctg aaa gaa gag atg tcc aag aac gga cgc 1075

Gly Leu Asn Thr Gly Glu Leu Lys Glu Glu Met Ser Lys Asn Gly Arg

310 315 320 325

atc ttc acc cgt gag ctc aac aag gac cgc gtc tac att ggc cgc aat 1123

Ile Phe Thr Arg Glu Leu Asn Lys Asp Arg Val Tyr Ile Gly Arg Asn

330 335 340

ggt acc gag ctg gtt ctg cac ggt cgt tcc ctg ctg ttc gtc cgc aac 1171

Gly Thr Glu Leu Val Leu His Gly Arg Ser Leu Leu Phe Val Arg Asn

345 350 355

gtt ggt cac ctc atg caa aac cca tcc atc ttg att gat ggc gag gag 1219

Val Gly His Leu Met Gln Asn Pro Ser Ile Leu Ile Asp Gly Glu Glu

360 365 370

atc ttc gaa ggc atc atg gat gct gtc ttg acc act gtt tgt gcc atc 1267

Ile Phe Glu Gly Ile Met Asp Ala Val Leu Thr Thr Val Cys Ala Ile

375 380 385

cca gga att gct ccg cag aac aag atg cgc aat tcc cgc aag ggc tcc 1315

Pro Gly Ile Ala Pro Gln Asn Lys Met Arg Asn Ser Arg Lys Gly Ser

390 395 400 405

atc tac atc gtg aag cct aag cag cac ggc cct gaa gaa gtc gcg ttc 1363

Ile Tyr Ile Val Lys Pro Lys Gln His Gly Pro Glu Glu Val Ala Phe

410 415 420

acc aac gag ctc ttc ggc cgc gtt gag gat ctg ctt gat ctg cca cgc 1411

Thr Asn Glu Leu Phe Gly Arg Val Glu Asp Leu Leu Asp Leu Pro Arg

425 430 435

cac acc ttg aag gtt ggt gtt atg gat gag gag cgt cgc acg tcc gtg 1459

His Thr Leu Lys Val Gly Val Met Asp Glu Glu Arg Arg Thr Ser Val

440 445 450

aac ctg gat gcc agc atc atg gaa gtt gct gac cgc ttg gca ttc atc 1507

Asn Leu Asp Ala Ser Ile Met Glu Val Ala Asp Arg Leu Ala Phe Ile

455 460 465

aac act ggc ttc ctg gac cgc acc ggc gat gaa atc cac acc tcc atg 1555

Asn Thr Gly Phe Leu Asp Arg Thr Gly Asp Glu Ile His Thr Ser Met

470 475 480 485

gaa gca ggc gcc atg gtg cgc aag gct gat atg cag acc gca ccg tgg 1603

Glu Ala Gly Ala Met Val Arg Lys Ala Asp Met Gln Thr Ala Pro Trp

490 495 500

aag cag gcc tac gag aac aac aac gtt gat gca ggt att cag cgt ggt 1651

Lys Gln Ala Tyr Glu Asn Asn Asn Val Asp Ala Gly Ile Gln Arg Gly

505 510 515

ctt cct ggc aag gct cag atc ggt aag ggc atg tgg gcg atg act gaa 1699

Leu Pro Gly Lys Ala Gln Ile Gly Lys Gly Met Trp Ala Met Thr Glu

520 525 530

ctc atg gca gaa atg ctg gag aag aag atc ggc cag cca cgc gaa ggc 1747

Leu Met Ala Glu Met Leu Glu Lys Lys Ile Gly Gln Pro Arg Glu Gly

535 540 545

gcc aac act gca tgg gtt cct tca cca act ggt gcg acg ctg cac gca 1795

Ala Asn Thr Ala Trp Val Pro Ser Pro Thr Gly Ala Thr Leu His Ala

550 555 560 565

acg cac tac cac ttg gtt gat gtg ttc aag gtt caa gac gaa ctg cgt 1843

Thr His Tyr His Leu Val Asp Val Phe Lys Val Gln Asp Glu Leu Arg

570 575 580

gct gcc ggc cgc cgc gac agc ctg cgc aac att ctc acc att cca acc 1891

Ala Ala Gly Arg Arg Asp Ser Leu Arg Asn Ile Leu Thr Ile Pro Thr

585 590 595

gca cca aac acc aat tgg tct gag gaa gag aag aag gaa gag atg gac 1939

Ala Pro Asn Thr Asn Trp Ser Glu Glu Glu Lys Lys Glu Glu Met Asp

600 605 610

aac aac tgc cag tcc atc ctc gga tac gtt gtg cgc tgg gtt gag cac 1987

Asn Asn Cys Gln Ser Ile Leu Gly Tyr Val Val Arg Trp Val Glu His

615 620 625

ggt gtt ggt tgc tcc aag gtt cca gac atc cat gac atc gac ctc atg 2035

Gly Val Gly Cys Ser Lys Val Pro Asp Ile His Asp Ile Asp Leu Met

630 635 640 645

gaa gac cgc gca acg ctg cgt att tcc tcg cag atg ctg gcc aac tgg 2083

Glu Asp Arg Ala Thr Leu Arg Ile Ser Ser Gln Met Leu Ala Asn Trp

650 655 660

atc cgc cat gat gtt gtc tcg aag gag cag gtc ttg gag tca ctg gaa 2131

Ile Arg His Asp Val Val Ser Lys Glu Gln Val Leu Glu Ser Leu Glu

665 670 675

cga atg gca gtg gtc gtc gac aag caa aat gcg ggc gac gag gcc tac 2179

Arg Met Ala Val Val Val Asp Lys Gln Asn Ala Gly Asp Glu Ala Tyr

680 685 690

cgc gat atg gcg ccg aac tac gac gcc tcc ctc gcc ttc cag gcg gct 2227

Arg Asp Met Ala Pro Asn Tyr Asp Ala Ser Leu Ala Phe Gln Ala Ala

695 700 705

aag gac ttg att ttc gaa ggc acc aag tcc cca tcg ggc tac acc gag 2275

Lys Asp Leu Ile Phe Glu Gly Thr Lys Ser Pro Ser Gly Tyr Thr Glu

710 715 720 725

ccc atc ttg cac gca cgc cgc cgc gag ttc aaa gca aaa aac 2317

Pro Ile Leu His Ala Arg Arg Arg Glu Phe Lys Ala Lys Asn

730 735

taagcacgct tttcgacgct tacctgcatc 2347

<210>78

<211>739

<212>PRT

<213>谷氨酸棒状杆菌

<400>78

Met Thr Glu Gln Glu Leu Leu Ser Ala Gln Thr Ala Asp Asn Ala Gly

1 5 10 15

Thr Asp Ser Thr Glu Arg Val Asp Ala Gly Gly Met Gln Val Ala Lys

20 25 30

Val Leu Tyr Asp Phe Val Thr Glu Ala Val Leu Pro Arg Val Gly Val

35 40 45

Asp Ala Glu Lys Phe Trp Ser Gly Phe Ala Ala Ile Ala Arg Asp Leu

50 55 60

Thr Pro Arg Asn Arg Glu Leu Leu Ala Arg Arg Asp Glu Leu Gln Met

65 70 75 80

Leu Ile Asp Asp Tyr His Arg Asn Asn Ser Gly Thr Ile Asp Gln Glu

85 90 95

Ala Tyr Glu Asp Phe Leu Lys Glu Ile Gly Tyr Leu Val Glu Glu Pro

100 105 110

Glu Ala Ala Glu Ile Arg Thr Gln Asn Val Asp Thr Glu Ile Ser Ser

115 120 125

Thr Ala Gly Pro Gln Leu Val Val Pro Ile Leu Asn Ala Arg Phe Ala

130 135 140

Leu Asn Ala Ala Asn Ala Arg Trp Gly Ser Leu Tyr Asp Ala Leu Tyr

145 150 155 160

Gly Thr Asn Ala Ile Pro Glu Thr Asp Gly Ala Glu Lys Gly Lys Glu

165 170 175

Tyr Asn Pro Val Arg Gly Gln Lys Val Ile Glu Trp Gly Arg Glu Phe

180 185 190

Leu Asp Ser Val Val Pro Leu Asp Gly Ala Ser His Ala Asp Val Glu

195 200 205

Lys Tyr Asn Ile Thr Asp Gly Lys Leu Ala Ala His Ile Gly Asp Ser

210 215 220

Val Tyr Arg Leu Lys Asn Arg Glu Ser Tyr Arg Gly Phe Thr Gly Asn

225 230 235 240

Phe Leu Asp Pro Glu Ala Ile Leu Leu Glu Thr Asn Gly Leu His Ile

245 250 255

Glu Leu Gln Ile Asp Pro Val His Pro Ile Gly Lys Ala Asp Lys Thr

260 265 270

Gly Leu Lys Asp Ile Val Leu Glu Ser Ala Ile Thr Thr Ile Met Asp

275 280 285

Phe Glu Asp Ser Val Ala Ala Val Asp Ala Glu Asp Lys Thr Leu Gly

290 295 300

Tyr Ser Asn Trp Phe Gly Leu Asn Thr Gly Glu Leu Lys Glu Glu Met

305 310 315 320

Ser Lys Asn Gly Arg Ile Phe Thr Arg Glu Leu Asn Lys Asp Arg Val

325 330 335

Tyr Ile Gly Arg Asn Gly Thr Glu Leu Val Leu His Gly Arg Ser Leu

340 345 350

Leu Phe Val Arg Asn Val Gly His Leu Met Gln Asn Pro Ser Ile Leu

355 360 365

Ile Asp Gly Glu Glu Ile Phe Glu Gly Ile Met Asp Ala Val Leu Thr

370 375 380

Thr Val Cys Ala Ile Pro Gly Ile Ala Pro Gln Asn Lys Met Arg Asn

385 390 395 400

Ser Arg Lys Gly Ser Ile Tyr Ile Val Lys Pro Lys Gln His Gly Pro

405 410 415

Glu Glu Val Ala Phe Thr Asn Glu Leu Phe Gly Arg Val Glu Asp Leu

420 425 430

Leu Asp Leu Pro Arg His Thr Leu Lys Val Gly Val Met Asp Glu Glu

435 440 445

Arg Arg Thr Ser Val Asn Leu Asp Ala Ser Ile Met Glu Val Ala Asp

450 455 460

Arg Leu Ala Phe Ile Asn Thr Gly Phe Leu Asp Arg Thr Gly Asp Glu

465 470 475 480

Ile His Thr Ser Met Glu Ala Gly Ala Met Val Arg Lys Ala Asp Met

485 490 495

Gln Thr Ala Pro Trp Lys Gln Ala Tyr Glu Asn Asn Asn Val Asp Ala

500 505 510

Gly Ile Gln Arg Gly Leu Pro Gly Lys Ala Gln Ile Gly Lys Gly Met

515 520 525

Trp Ala Met Thr Glu Leu Met Ala Glu Met Leu Glu Lys Lys Ile Gly

530 535 540

Gln Pro Arg Glu Gly Ala Asn Thr Ala Trp Val Pro Ser Pro Thr Gly

545 550 555 560

Ala Thr Leu His Ala Thr His Tyr His Leu Val Asp Val Phe Lys Val

565 570 575

Gln Asp Glu Leu Arg Ala Ala Gly Arg Arg Asp Ser Leu Arg Asn Ile

580 585 590

Leu Thr Ile Pro Thr Ala Pro Asn Thr Asn Trp Ser Glu Glu Glu Lys

595 600 605

Lys Glu Glu Met Asp Asn Asn Cys Gln Ser Ile Leu Gly Tyr Val Val

610 615 620

Arg Trp Val Glu His Gly Val Gly Cys Ser Lys Val Pro Asp Ile His

625 630 635 640

Asp Ile Asp Leu Met Glu Asp Arg Ala Thr Leu Arg Ile Ser Ser Gln

645 650 655

Met Leu Ala Asn Trp Ile Arg His Asp Val Val Ser Lys Glu Gln Val

660 665 670

Leu Glu Ser Leu Glu Arg Met Ala Val Val Val Asp Lys Gln Asn Ala

675 680 685

Gly Asp Glu Ala Tyr Arg Asp Met Ala Pro Asn Tyr Asp Ala Ser Leu

690 695 700

Ala Phe Gln Ala Ala Lys Asp Leu Ile Phe Glu Gly Thr Lys Ser Pro

705 710 715 720

Ser Gly Tyr Thr Glu Pro Ile Leu His Ala Arg Arg Arg Glu Phe Lys

725 730 735

Ala Lys Asn

<210>79

<211>877

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(847)

<223>RXA02414

<400>79

tgtcggctca tgctgcagat tgcgtacagg acgctatcat cgacgccata cttaccgcga 60

gtccgggact cgggctcaaa agcttcaggg aacttttacc atg agt tac aac agc 115

Met Ser Tyr Asn Ser

1 5

ccg tat aac aac acg aat ttc agc acc act ggc gcg ttc caa cct gct 163

Pro Tyr Asn Asn Thr Asn Phe Ser Thr Thr Gly Ala Phe Gln Pro Ala

10 15 20

ggt ggg ccg gtg aag ccg tgg aat aag ccc gat gcc agc ctg aat cag 211

Gly Gly Pro Val Lys Pro Trp Asn Lys Pro Asp Ala Ser Leu Asn Gln

25 30 35

cag ctg aaa aac aaa tcc cgt gtg cgc aca ggt ctt acc atc gcc atc 259

Gln Leu Lys Asn Lys Ser Arg Val Arg Thr Gly Leu Thr Ile Ala Ile

40 45 50

ggt tat gta gtg gtg att tgg gcg gtg cat ttg gca tcc atc gtc att 307

Gly Tyr Val Val Val Ile Trp Ala Val His Leu Ala Ser Ile Val Ile

55 60 65

gcg ctg ctc act ggc ttc aac ctg acc aac ttt ggt att cat ccg ctg 355

Ala Leu Leu Thr Gly Phe Asn Leu Thr Asn Phe Gly Ile His Pro Leu

70 75 80 85

gat acc agt gca ctg tgg ggt att ttc acc tca ccg ctg ttg cat gga 403

Asp Thr Ser Ala Leu Trp Gly Ile Phe Thr Ser Pro Leu Leu His Gly

90 95 100

agc ttc agc cac ctc att gga aat acc gtt cca ggc ttt ata ttc agt 451

Ser Phe Ser His Leu Ile Gly Asn Thr Val Pro Gly Phe Ile Phe Ser

105 110 115

ttc ctc atc ggt atg agt ggc aag cgc gtg ttc tgg gaa gtc acg att 499

Phe Leu Ile Gly Met Ser Gly Lys Arg Val Phe Trp Glu Val Thr Ile

120 125 130

atc gca ggt ctc atc ggc ggt ctt ggt aca tgg att ttc ggt gga atc 547

Ile Ala Gly Leu Ile Gly Gly Leu Gly Thr Trp Ile Phe Gly Gly Ile

135 140 145

ggc acc aac cac atc ggt gcg tcc ggc ctg att tat ggc tgg ctt ggc 595

Gly Thr Asn His Ile Gly Ala Ser Gly Leu Ile Tyr Gly Trp Leu Gly

150 155 160 165

tac ctg atc gtg cgt gga att ttc aac aag gat att aaa cag ttc ctg 643

Tyr Leu Ile Val Arg Gly Ile Phe Asn Lys Asp Ile Lys Gln Phe Leu

170 175 180

ctt ggc ata gtt ttg gcg ttc att tac tcc ggt ttg ttc tgg ggt ctg 691

Leu Gly Ile Val Leu Ala Phe Ile Tyr Ser Gly Leu Phe Trp Gly Leu

185 190 195

cta cct act caa atc ggt gtg tcc tgg cag ggc cac ctt ttc ggt gca 739

Leu Pro Thr Gln Ile Gly Val Ser Trp Gln Gly His Leu Phe Gly Ala

200 205 210

ctt ggt gga atc ggt gcg ggt gct ttt atc gcc tcg gat gac ccg gca 787

Leu Gly Gly Ile Gly Ala Gly Ala Phe Ile Ala Ser Asp Asp Pro Ala

215 220 225

gcg ttg aaa gcg aag aag caa cag aag aaa tta gaa aag caa caa cgc 835

Ala Leu Lys Ala Lys Lys Gln Gln Lys Lys Leu Glu Lys Gln Gln Arg

230 235 240 245

caa aga ggc tta tagttttcac ctagcgacta cacttaattg 877

Gln Arg Gly Leu

<210>80

<211>249

<212>PRT

<213>谷氨酸棒状杆菌

<400>80

Met Ser Tyr Asn Ser Pro Tyr Asn Asn Thr Asn Phe Ser Thr Thr Gly

1 5 10 15

Ala Phe Gln Pro Ala Gly Gly Pro Val Lys Pro Trp Asn Lys Pro Asp

20 25 30

Ala Ser Leu Asn Gln Gln Leu Lys Asn Lys Ser Arg Val Arg Thr Gly

35 40 45

Leu Thr Ile Ala Ile Gly Tyr Val Val Val Ile Trp Ala Val His Leu

50 55 60

Ala Ser Ile Val Ile Ala Leu Leu Thr Gly Phe Asn Leu Thr Asn Phe

65 70 75 80

Gly Ile His Pro Leu Asp Thr Ser Ala Leu Trp Gly Ile Phe Thr Ser

85 90 95

Pro Leu Leu His Gly Ser Phe Ser His Leu Ile Gly Asn Thr Val Pro

100 105 110

Gly Phe Ile Phe Ser Phe Leu Ile Gly Met Ser Gly Lys Arg Val Phe

115 120 125

Trp Glu Val Thr Ile Ile Ala Gly Leu Ile Gly Gly Leu Gly Thr Trp

130 135 140

Ile Phe Gly Gly Ile Gly Thr Asn His Ile Gly Ala Ser Gly Leu Ile

145 150 155 160

Tyr Gly Trp Leu Gly Tyr Leu Ile Val Arg Gly Ile Phe Asn Lys Asp

165 170 175

Ile Lys Gln Phe Leu Leu Gly Ile Val Leu Ala Phe Ile Tyr Ser Gly

180 185 190

Leu Phe Trp Gly Leu Leu Pro Thr Gln Ile Gly Val Ser Trp Gln Gly

195 200 205

His Leu Phe Gly Ala Leu Gly Gly Ile Gly Ala Gly Ala Phe Ile Ala

210 215 220

Ser Asp Asp Pro Ala Ala Leu Lys Ala Lys Lys Gln Gln Lys Lys Leu

225 230 235 240

Glu Lys Gln Gln Arg Gln Arg Gly Leu

245

<210>81

<211>1780

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1750)

<223>RXA02434

<400>81

cttcaatagt caaaaccagc aaactaattt tttaagtttt acgtaactgg ccccaccgct 60

tgtggcaggc cttgcgtttt gacattgaag gacccttttt atg cgc act ttt gcc 115

Met Arg Thr Phe Ala

1 5

gct tat att gcc att gat ggc ctc agc ttt tcc tac ccc aac acc cac 163

Ala Tyr Ile Ala Ile Asp Gly Leu Ser Phe Ser Tyr Pro Asn Thr His

10 15 20

gtt tta agc gat att tcg ctc acc gtt gcc aat ggc gat atc gcc gga 211

Val Leu Ser Asp Ile Ser Leu Thr Val Ala Asn Gly Asp Ile Ala Gly

25 30 35

ctg att ggt gaa aac ggc gca gga aaa tcc acc ctg ctc agc ctc atc 259

Leu Ile Gly Glu Asn Gly Ala Gly Lys Ser Thr Leu Leu Ser Leu Ile

40 45 50

gct ggc gtc atg gaa ccc gac cag ggc agg att tac ctc ccc gaa cgc 307

Ala Gly Val Met Glu Pro Asp Gln Gly Arg Ile Tyr Leu Pro Glu Arg

55 60 65

acc gga ttc atc gcc caa gaa aca gac tta ccg ttt gaa caa ccc gtg 355

Thr Gly Phe Ile Ala Gln Glu Thr Asp Leu Pro Phe Glu Gln Pro Val

70 75 80 85

cag tcg ctt atc gac gcc gcc gtc gcc cca gtg cgc gcg gtc gat gcc 403

Gln Ser Leu Ile Asp Ala Ala Val Ala Pro Val Arg Ala Val Asp Ala

90 95 100

gcg att aca gat ttg tcc acc aag ctt ggc gac gcc tcc ctc agc gcc 451

Ala Ile Thr Asp Leu Ser Thr Lys Leu Gly Asp Ala Ser Leu Ser Ala

105 110 115

gaa gag cag gcg caa gtc gcc aca gat ttc gat gca gcg cta ggc gct 499

Glu Glu Gln Ala Gln Val Ala Thr Asp Phe Asp Ala Ala Leu Gly Ala

120 125 130

gca gaa gaa ctc gga ctg tgg gaa tta gat gca cgt att gaa acc atc 547

Ala Glu Glu Leu Gly Leu Trp Glu Leu Asp Ala Arg Ile Glu Thr Ile

135 140 145

gtc gcg ggt ctc ggc ctt gcc gag gtg gat cgc agc act ccc att ggt 595

Val Ala Gly Leu Gly Leu Ala Glu Val Asp Arg Ser Thr Pro Ile Gly

150 155 160 165

gag ctt tcc ggc ggt cag cgc cgc aga ttc gca ttg gca gcg ctg ctg 643

Glu Leu Ser Gly Gly Gln Arg Arg Arg Phe Ala Leu Ala Ala Leu Leu

170 175 180

ttg gaa cca cac gat gct ctg att ttc gat gag ccc acc aac cac ctc 691

Leu Glu Pro His Asp Ala Leu Ile Phe Asp Glu Pro Thr Asn His Leu

185 190 195

gac gac aca gcc gta gat ttc ctc atc tcg gag att tcc cgt ttc aaa 739

Asp Asp Thr Ala Val Asp Phe Leu Ile Ser Glu Ile Ser Arg Phe Lys

200 205 210

ggt cca gtg ctg atc gcc agc cac gat cgc ttc ttc ctc gac tcc gtc 787

Gly Pro Val Leu Ile Ala Ser His Asp Arg Phe Phe Leu Asp Ser Val

215 220 225

tgt acc gag tta atc gac ctc gat cct gca ctt gga cct gag ggc gga 835

Cys Thr Glu Leu Ile Asp Leu Asp Pro Ala Leu Gly Pro Glu Gly Gly

230 235 240 245

tcc ggc gaa gaa gta aaa caa gcc gtg tct ttt ggt ggt gga ttt tct 883

Ser Gly Glu Glu Val Lys Gln Ala Val Ser Phe Gly Gly Gly Phe Ser

250 255 260

gaa tac atc aaa gaa cgc gag acc cgc cgc acc cgc tgg gct cag ttg 931

Glu Tyr Ile Lys Glu Arg Glu Thr Arg Arg Thr Arg Trp Ala Gln Leu

265 270 275

tac acc gca caa gaa acc gag cgg gaa aaa ctc gaa gaa acc acc ggc 979

Tyr Thr Ala Gln Glu Thr Glu Arg Glu Lys Leu Glu Glu Thr Thr Gly

280 285 290

acc acc gaa tcg gat att ttc cac agc tcg gtt tcc aaa tcg gaa gct 1027

Thr Thr Glu Ser Asp Ile Phe His Ser Ser Val Ser Lys Ser Glu Ala

295 300 305

aaa atc acc gcg aaa ttt tac gca gac cgg gca gct aaa act caa ggc 1075

Lys Ile Thr Ala Lys Phe Tyr Ala Asp Arg Ala Ala Lys Thr Gln Gly

310 315 320 325

aac cgc gtc cgc tcc gcc aaa aac cgc ctg aag gaa ttg gaa cgc tat 1123

Asn Arg Val Arg Ser Ala Lys Asn Arg Leu Lys Glu Leu Glu Arg Tyr

330 335 340

gaa atc cca gca cct cca aag cca ctg gaa ttc caa ggc atc cca gaa 1171

Glu Ile Pro Ala Pro Pro Lys Pro Leu Glu Phe Gln Gly Ile Pro Glu

345 350 355

gcc tcc gga aac ggt cac ggt gaa aca cta gaa gtg cgg gct att gct 1219

Ala Ser Gly Asn Gly His Gly Glu Thr Leu Glu Val Arg Ala Ile Ala

360 365 370

gtg gaa aac agg ctt caa ccc ttg act ttc cac atc gat ccc ggc gac 1267

Val Glu Asn Arg Leu Gln Pro Leu Thr Phe His Ile Asp Pro Gly Asp

375 380 385

cac atc ctg gtc gaa ggc ccc aac ggt gtc ggt aaa tcc acc ctg ctg 1315

His Ile Leu Val Glu Gly Pro Asn Gly Val Gly Lys Ser Thr Leu Leu

390 395 400 405

agc gtt ctg gaa ggc gtg ctt gaa cca acc gaa ggt gaa ttg atc gtc 1363

Ser Val Leu Glu Gly Val Leu Glu Pro Thr Glu Gly Glu Leu Ile Val

410 415 420

ccc gaa ggg ctg aaa gtt gcg cgc ctg aaa cag gac gat cag tgg acg 1411

Pro Glu Gly Leu Lys Val Ala Arg Leu Lys Gln Asp Asp Gln Trp Thr

425 430 435

gaa aag cag ttg aac acc ccc gtc gac gaa ctg ttc gcc gcc cta tcg 1459

Glu Lys Gln Leu Asn Thr Pro Val Asp Glu Leu Phe Ala Ala Leu Ser

440 445 450

aaa ggt ccg gtc gga ctc aac ctc gtg gag atg ggg ctg ttg agg gag 1507

Lys Gly Pro Val Gly Leu Asn Leu Val Glu Met Gly Leu Leu Arg Glu

455 460 465

acg tcg caa agc agc ccg cta cgg gcc cta tcg ctc ggc caa cgc cgg 1555

Thr Ser Gln Ser Ser Pro Leu Arg Ala Leu Ser Leu Gly Gln Arg Arg

470 475 480 485

cgc gtc tcg ctc ggg ctc atc ctg gcg agc cca cca gat ctt ttg ctt 1603

Arg Val Ser Leu Gly Leu Ile Leu Ala Ser Pro Pro Asp Leu Leu Leu

490 495 500

ctt gac gag ccc acc aac cac ctc tcc ctc gcg ctg agc gaa gaa ctc 1651

Leu Asp Glu Pro Thr Asn His Leu Ser Leu Ala Leu Ser Glu Glu Leu

505 510 515

gag tcg gcg ata gaa aaa ttc ccc ggt cgc gtt att ctg gcc agc cac 1699

Glu Ser Ala Ile Glu Lys Phe Pro Gly Arg Val Ile Leu Ala Ser His

520 525 530

gat agg tgg atc aga aaa cgt tgg acg ggg aag aaa atc agc ctg agc 1747

Asp Arg Trp Ile Arg Lys Arg Trp Thr Gly Lys Lys Ile Ser Leu Ser

535 540 545

cgt taaaccctac tgaacaggaa cctcttcaac 1780

Arg

550

<210>82

<211>550

<212>PRT

<213>谷氨酸棒状杆菌

<400>82

Met Arg Thr Phe Ala Ala Tyr Ile Ala Ile Asp Gly Leu Ser Phe Ser

1 5 10 15

Tyr Pro Asn Thr His Val Leu Ser Asp Ile Ser Leu Thr Val Ala Asn

20 25 30

Gly Asp Ile Ala Gly Leu Ile Gly Glu Asn Gly Ala Gly Lys Ser Thr

35 40 45

Leu Leu Ser Leu Ile Ala Gly Val Met Glu Pro Asp Gln Gly Arg Ile

50 55 60

Tyr Leu Pro Glu Arg Thr Gly Phe Ile Ala Gln Glu Thr Asp Leu Pro

65 70 75 80

Phe Glu Gln Pro Val Gln Ser Leu Ile Asp Ala Ala Val Ala Pro Val

85 90 95

Arg Ala Val Asp Ala Ala Ile Thr Asp Leu Ser Thr Lys Leu Gly Asp

100 105 110

Ala Ser Leu Ser Ala Glu Glu Gln Ala Gln Val Ala Thr Asp Phe Asp

115 120 125

Ala Ala Leu Gly Ala Ala Glu Glu Leu Gly Leu Trp Glu Leu Asp Ala

130 135 140

Arg Ile Glu Thr Ile Val Ala Gly Leu Gly Leu Ala Glu Val Asp Arg

145 150 155 160

Ser Thr Pro Ile Gly Glu Leu Ser Gly Gly Gln Arg Arg Arg Phe Ala

165 170 175

Leu Ala Ala Leu Leu Leu Glu Pro His Asp Ala Leu Ile Phe Asp Glu

180 185 190

Pro Thr Asn His Leu Asp Asp Thr Ala Val Asp Phe Leu Ile Ser Glu

195 200 205

Ile Ser Arg Phe Lys Gly Pro Val Leu Ile Ala Ser His Asp Arg Phe

210 215 220

Phe Leu Asp Ser Val Cys Thr Glu Leu Ile Asp Leu Asp Pro Ala Leu

225 230 235 240

Gly Pro Glu Gly Gly Ser Gly Glu Glu Val Lys Gln Ala Val Ser Phe

245 250 255

Gly Gly Gly Phe Ser Glu Tyr Ile Lys Glu Arg Glu Thr Arg Arg Thr

260 265 270

Arg Trp Ala Gln Leu Tyr Thr Ala Gln Glu Thr Glu Arg Glu Lys Leu

275 280 285

Glu Glu Thr Thr Gly Thr Thr Glu Ser Asp Ile Phe His Ser Ser Val

290 295 300

Ser Lys Ser Glu Ala Lys Ile Thr Ala Lys Phe Tyr Ala Asp Arg Ala

305 310 315 320

Ala Lys Thr Gln Gly Asn Arg Val Arg Ser Ala Lys Asn Arg Leu Lys

325 330 335

Glu Leu Glu Arg Tyr Glu Ile Pro Ala Pro Pro Lys Pro Leu Glu Phe

340 345 350

Gln Gly Ile Pro Glu Ala Ser Gly Asn Gly His Gly Glu Thr Leu Glu

355 360 365

Val Arg Ala Ile Ala Val Glu Asn Arg Leu Gln Pro Leu Thr Phe His

370 375 380

Ile Asp Pro Gly Asp His Ile Leu Val Glu Gly Pro Asn Gly Val Gly

385 390 395 400

Lys Ser Thr Leu Leu Ser Val Leu Glu Gly Val Leu Glu Pro Thr Glu

405 410 415

Gly Glu Leu Ile Val Pro Glu Gly Leu Lys Val Ala Arg Leu Lys Gln

420 425 430

Asp Asp Gln Trp Thr Glu Lys Gln Leu Asn Thr Pro Val Asp Glu Leu

435 440 445

Phe Ala Ala Leu Ser Lys Gly Pro Val Gly Leu Asn Leu Val Glu Met

450 455 460

Gly Leu Leu Arg Glu Thr Ser Gln Ser Ser Pro Leu Arg Ala Leu Ser

465 470 475 480

Leu Gly Gln Arg Arg Arg Val Ser Leu Gly Leu Ile Leu Ala Ser Pro

485 490 495

Pro Asp Leu Leu Leu Leu Asp Glu Pro Thr Asn His Leu Ser Leu Ala

500 505 510

Leu Ser Glu Glu Leu Glu Ser Ala Ile Glu Lys Phe Pro Gly Arg Val

515 520 525

Ile Leu Ala Ser His Asp Arg Trp Ile Arg Lys Arg Trp Thr Gly Lys

530 535 540

Lys Ile Ser Leu Ser Arg

545 550

<210>83

<211>1111

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1081)

<223>RXA02440

<400>83

ggaaaaagga atcataaagc cacagcgcaa agccactgaa tcaataaaga agcgttaata 60

aagtttgact tgtgcctctg acctgcgttg acttgagtaa atg aag cta cca aga 115

Met Lys Leu Pro Arg

1 5

ctc gcc gca gcg ctc att ggt gct gcg ctg tta atc acg ggt tgt tca 163

Leu Ala Ala Ala Leu Ile Gly Ala Ala Leu Leu Ile Thr Gly Cys Ser

10 15 20

gct acc ggg gga gca cca cgg gca aca gat ggg gca tca ggc gga gga 211

Ala Thr Gly Gly Ala Pro Arg Ala Thr Asp Gly Ala Ser Gly Gly Gly

25 30 35

acc gtc gat acg cct cgg ttg gtt gtc gcg atg gtg agc cac ggc gcg 259

Thr Val Asp Thr Pro Arg Leu Val Val Ala Met Val Ser His Gly Ala

40 45 50

ccg ggc gat act ttt tgg gat ttg gtc cga aaa ggt gct gaa gac gcc 307

Pro Gly Asp Thr Phe Trp Asp Leu Val Arg Lys Gly Ala Glu Asp Ala

55 60 65

gcc caa aaa gac aac gtt gaa ctc cgc tat tcc tct aat ccg gaa atc 355

Ala Gln Lys Asp Asn Val Glu Leu Arg Tyr Ser Ser Asn Pro Glu Ile

70 75 80 85

cct gaa caa tcc aac ctc gtg caa aat gcc atc gat tca cgc gtc gac 403

Pro Glu Gln Ser Asn Leu Val Gln Asn Ala Ile Asp Ser Arg Val Asp

90 95 100

ggc atc gcc atg acc atg cct aat gct caa tca cta gga ccg gtc gct 451

Gly Ile Ala Met Thr Met Pro Asn Ala Gln Ser Leu Gly Pro Val Ala

105 110 115

caa aag gcc gtg gat gcg ggc att cct gtg gtt ggt ctc aac gct gga 499

Gln Lys Ala Val Asp Ala Gly Ile Pro Val Val Gly Leu Asn Ala Gly

120 125 130

atg aac gaa tac caa gat tat gga atg aca gga ttc ttt ggt caa gat 547

Met Asn Glu Tyr Gln Asp Tyr Gly Met Thr Gly Phe Phe Gly Gln Asp

135 140 145

gaa tcc gtc gca gga gca tcc gca gga gcg cgc ctt gcc gag gaa aac 595

Glu Ser Val Ala Gly Ala Ser Ala Gly Ala Arg Leu Ala Glu Glu Asn

150 155 160 165

gca caa aaa gtt ttg tgt gtg atc cat gaa cag ggc aac tcc tcc cag 643

Ala Gln Lys Val Leu Cys Val Ile His Glu Gln Gly Asn Ser Ser Gln

170 175 180

gaa gct cgc tgt ggt ggc gtg tct gaa ggt ttg ggc aaa caa gta gaa 691

Glu Ala Arg Cys Gly Gly Val Ser Glu Gly Leu Gly Lys Gln Val Glu

185 190 195

acc ctg tat gtc aac ggc atg gat ctc acc tca gtg aac tcc acc ctg 739

Thr Leu Tyr Val Asn Gly Met Asp Leu Thr Ser Val Asn Ser Thr Leu

200 205 210

cag gca aaa ctt gct caa gac cgc agc att gat tgg gtt gtg gga ctc 787

Gln Ala Lys Leu Ala Gln Asp Arg Ser Ile Asp Trp Val Val Gly Leu

215 220 225

cag gct ggt gta tca atg gct att tct gat gcg gca gac gct gcg aac 835

Gln Ala Gly Val Ser Met Ala Ile Ser Asp Ala Ala Asp Ala Ala Asn

230 235 240 245

tca gaa gta aag atc gcc acc ttt gat aca aac gca cag ctc atg acc 883

Ser Glu Val Lys Ile Ala Thr Phe Asp Thr Asn Ala Gln Leu Met Thr

250 255 260

gct att cgt gat ggc aag atc caa ttc gcc att gat cag caa cca tat 931

Ala Ile Arg Asp Gly Lys Ile Gln Phe Ala Ile Asp Gln Gln Pro Tyr

265 270 275

ctg cag ggc tac atg gcc gtg gat tcg ctg tgg ttg gcg cac cga aac 979

Leu Gln Gly Tyr Met Ala Val Asp Ser Leu Trp Leu Ala His Arg Asn

280 285 290

ggc acc act gtt ggt ggc gga cga ccc gtg tac aca gga cca gcc att 1027

Gly Thr Thr Val Gly Gly Gly Arg Pro Val Tyr Thr Gly Pro Ala Ile

295 300 305

gtg gat gcc acc aac gtt gat gtc att gct gaa gcc gtt ggg gag ggt 1075

Val Asp Ala Thr Asn Val Asp Val Ile Ala Glu Ala Val Gly Glu Gly

310 315 320 325

ctg cga tgacaaaaat caagagtggg gaggcgtcga 1111

Leu Arg

<210>84

<211>327

<212>PRT

<213>谷氨酸棒状杆菌

<400>84

Met Lys Leu Pro Arg Leu Ala Ala Ala Leu Ile Gly Ala Ala Leu Leu

1 5 10 15

Ile Thr Gly Cys Ser Ala Thr Gly Gly Ala Pro Arg Ala Thr Asp Gly

20 25 30

Ala Ser Gly Gly Gly Thr Val Asp Thr Pro Arg Leu Val Val Ala Met

35 40 45

Val Ser His Gly Ala Pro Gly Asp Thr Phe Trp Asp Leu Val Arg Lys

50 55 60

Gly Ala Glu Asp Ala Ala Gln Lys Asp Asn Val Glu Leu Arg Tyr Ser

65 70 75 80

Ser Asn Pro Glu Ile Pro Glu Gln Ser Asn Leu Val Gln Asn Ala Ile

85 90 95

Asp Ser Arg Val Asp Gly Ile Ala Met Thr Met Pro Asn Ala Gln Ser

100 105 110

Leu Gly Pro Val Ala Gln Lys Ala Val Asp Ala Gly Ile Pro Val Val

115 120 125

Gly Leu Asn Ala Gly Met Asn Glu Tyr Gln Asp Tyr Gly Met Thr Gly

130 135 140

Phe Phe Gly Gln Asp Glu Ser Val Ala Gly Ala Ser Ala Gly Ala Arg

145 150 155 160

Leu Ala Glu Glu Asn Ala Gln Lys Val Leu Cys Val Ile His Glu Gln

165 170 175

Gly Asn Ser Ser Gln Glu Ala Arg Cys Gly Gly Val Ser Glu Gly Leu

180 185 190

Gly Lys Gln Val Glu Thr Leu Tyr Val Asn Gly Met Asp Leu Thr Ser

195 200 205

Val Asn Ser Thr Leu Gln Ala Lys Leu Ala Gln Asp Arg Ser Ile Asp

210 215 220

Trp Val Val Gly Leu Gln Ala Gly Val Ser Met Ala Ile Ser Asp Ala

225 230 235 240

Ala Asp Ala Ala Asn Ser Glu Val Lys Ile Ala Thr Phe Asp Thr Asn

245 250 255

Ala Gln Leu Met Thr Ala Ile Arg Asp Gly Lys Ile Gln Phe Ala Ile

260 265 270

Asp Gln Gln Pro Tyr Leu Gln Gly Tyr Met Ala Val Asp Ser Leu Trp

275 280 285

Leu Ala His Arg Asn Gly Thr Thr Val Gly Gly Gly Arg Pro Val Tyr

290 295 300

Thr Gly Pro Ala Ile Val Asp Ala Thr Asn Val Asp Val Ile Ala Glu

305 310 315 320

Ala Val Gly Glu Gly Leu Arg

325

<210>85

<211>940

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(910)

<223>RXA02474

<400>85

ataatcttca tcacatggtg tgtgcagaaa tatttttgct ggtctattgt ggcgaccgag 60

ggcctttgaa ggttcgacaa actgtataag gccttgaatc ttg aga att tat ttt 115

Leu Arg Ile Tyr Phe

1 5

gag gaa gca aga gga agt gtc atg agc aaa gtt gca atg gtt acc ggt 163

Glu Glu Ala Arg Gly Ser Val Met Ser Lys Val Ala Met Val Thr Gly

10 15 20

ggt gca caa ggc atc ggt cgt gga att tca gag aag ctg gca gca gat 211

Gly Ala Gln Gly Ile Gly Arg Gly Ile Ser Glu Lys Leu Ala Ala Asp

25 30 35

ggt ttc gat att gcc gta gcc gac ctg cca caa cag gaa gaa caa gct 259

Gly Phe Asp Ile Ala Val Ala Asp Leu Pro Gln Gln Glu Glu Gln Ala

40 45 50

gca gag acc atc aag ttg att gaa gct gca ggt caa aag gct gta ttc 307

Ala Glu Thr Ile Lys Leu Ile Glu Ala Ala Gly Gln Lys Ala Val Phe

55 60 65

gtt gga tta gat gtc acc gat aag gct aat ttc gac agt gca att gat 355

Val Gly Leu Asp Val Thr Asp Lys Ala Asn Phe Asp Ser Ala Ile Asp

70 75 80 85

gag gca gca gag aaa ctt ggc ggc ttc gat gtg cta gta aac aac gcc 403

Glu Ala Ala Glu Lys Leu Gly Gly Phe Asp Val Leu Val Asn Asn Ala

90 95 100

ggc atc gca caa att aag cca ctt ctg gaa gtc acc gaa gaa gac cta 451

Gly Ile Ala Gln Ile Lys Pro Leu Leu Glu Val Thr Glu Glu Asp Leu

105 110 115

aag cag atc tac tcc gtg aac gtt ttt agc gta ttt ttt ggt att caa 499

Lys Gln Ile Tyr Ser Val Asn Val Phe Ser Val Phe Phe Gly Ile Gln

120 125 130

gca gca tcc cga aag ttc gat gag ctt ggc gta aaa ggc aag atc atc 547

Ala Ala Ser Arg Lys Phe Asp Glu Leu Gly Val Lys Gly Lys Ile Ile

135 140 145

aac gct gca tca atc gct gct atc caa ggt ttc cca atc ttg agc gcc 595

Asn Ala Ala Ser Ile Ala Ala Ile Gln Gly Phe Pro Ile Leu Ser Ala

150 155 160 165

tac tcc acc acc aaa ttc gcg gtt cgt ggc ctc acc cag gct gct gcg 643

Tyr Ser Thr Thr Lys Phe Ala Val Arg Gly Leu Thr Gln Ala Ala Ala

170 175 180

caa gaa ctc gca ccc aag ggt cac acc gtg aat gcc tac gca cct ggc 691

Gln Glu Leu Ala Pro Lys Gly His Thr Val Asn Ala Tyr Ala Pro Gly

185 190 195

atc gtg ggc acc gga atg tgg gag caa atc gat gcc gag ctt tcc aag 739

Ile Val Gly Thr Gly Met Trp Glu Gln Ile Asp Ala Glu Leu Ser Lys

200 205 210

atc aac ggc aag cca atc ggt gag aac ttc aag gag tac tcc tcc tca 787

Ile Asn Gly Lys Pro Ile Gly Glu Asn Phe Lys Glu Tyr Ser Ser Ser

215 220 225

atc gca ttg ggc cga cca tca gta cct gag gat gta gcc ggt ctg gtt 835

Ile Ala Leu Gly Arg Pro Ser Val Pro Glu Asp Val Ala Gly Leu Val

230 235 240 245

tcg ttc ctg gct tct gaa aac tcc aac tac atc acc gga cag gtc atg 883

Ser Phe Leu Ala Ser Glu Asn Ser Asn Tyr Ile Thr Gly Gln Val Met

250 255 260

ctt gtc gac ggc ggc atg ctc tac aac taggggttgc tttcccgcac 930

Leu Val Asp Gly Gly Met Leu Tyr Asn

265 270

tcaacgcagt 940

<210>86

<211>270

<212>PRT

<213>谷氨酸棒状杆菌

<400>86

Leu Arg Ile Tyr Phe Glu Glu Ala Arg Gly Ser Val Met Ser Lys Val

1 5 10 15

Ala Met Val Thr Gly Gly Ala Gln Gly Ile Gly Arg Gly Ile Ser Glu

20 25 30

Lys Leu Ala Ala Asp Gly Phe Asp Ile Ala Val Ala Asp Leu Pro Gln

35 40 45

Gln Glu Glu Gln Ala Ala Glu Thr Ile Lys Leu Ile Glu Ala Ala Gly

50 55 60

Gln Lys Ala Val Phe Val Gly Leu Asp Val Thr Asp Lys Ala Asn Phe

65 70 75 80

Asp Ser Ala Ile Asp Glu Ala Ala Glu Lys Leu Gly Gly Phe Asp Val

85 90 95

Leu Val Asn Asn Ala Gly Ile Ala Gln Ile Lys Pro Leu Leu Glu Val

100 105 110

Thr Glu Glu Asp Leu Lys Gln Ile Tyr Ser Val Asn Val Phe Ser Val

115 120 125

Phe Phe Gly Ile Gln Ala Ala Ser Arg Lys Phe Asp Glu Leu Gly Val

130 135 140

Lys Gly Lys Ile Ile Asn Ala Ala Ser Ile Ala Ala Ile Gln Gly Phe

145 150 155 160

Pro Ile Leu Ser Ala Tyr Ser Thr Thr Lys Phe Ala Val Arg Gly Leu

165 170 175

Thr Gln Ala Ala Ala Gln Glu Leu Ala Pro Lys Gly His Thr Val Asn

180 185 190

Ala Tyr Ala Pro Gly Ile Val Gly Thr Gly Met Trp Glu Gln Ile Asp

195 200 205

Ala Glu Leu Ser Lys Ile Asn Gly Lys Pro Ile Gly Glu Asn Phe Lys

210 215 220

Glu Tyr Ser Ser Ser Ile Ala Leu Gly Arg Pro Ser Val Pro Glu Asp

225 230 235 240

Val Ala Gly Leu Val Ser Phe Leu Ala Ser Glu Asn Ser Asn Tyr Ile

245 250 255

Thr Gly Gln Val Met Leu Val Asp Gly Gly Met Leu Tyr Asn

260 265 270

<210>87

<211>1882

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1852)

<223>RXA02480

<400>87

ttgggttggc atcggatcct gcctgtggcc taagatcagg cagtgttgtt aaaggacgat 60

cggtaatccg aatggatcgt cccgtagtca ggaggaacct atg acc gct gtg gcg 115

Met Thr Ala Val Ala

1 5

cct agg gtc gac ggg cac gtc gcc cct cag agg ccc gag ccg aca ggc 163

Pro Arg Val Asp Gly His Val Ala Pro Gln Arg Pro Glu Pro Thr Gly

10 15 20

cat gca cgc aag ggc agc aaa gca tgg tta atg atg acc acc acc gac 211

His Ala Arg Lys Gly Ser Lys Ala Trp Leu Met Met Thr Thr Thr Asp

25 30 35

cac aag cag ctg ggc att atg tac atc att atg tcc ttc agc ttc ttc 259

His Lys Gln Leu Gly Ile Met Tyr Ile Ile Met Ser Phe Ser Phe Phe

40 45 50

ttc ctc ggt ggc ttg atg gcc ctg ctt atc cga gcg gag ctt ttc acc 307

Phe Leu Gly Gly Leu Met Ala Leu Leu Ile Arg Ala Glu Leu Phe Thr

55 60 65

cct ggt ctg cag ttc ctg tct aat gag cag ttc aac cag ctg ttc acc 355

Pro Gly Leu Gln Phe Leu Ser Asn Glu Gln Phe Asn Gln Leu Phe Thr

70 75 80 85

atg cac gga act gtc atg ctg ctg ctg tac gga act cca att gtt tgg 403

Met His Gly Thr Val Met Leu Leu Leu Tyr Gly Thr Pro Ile Val Trp

90 95 100

ggt ttt gct aac tac gtc ctg cca ctt cag atc ggt gcg cct gac gta 451

Gly Phe Ala Asn Tyr Val Leu Pro Leu Gln Ile Gly Ala Pro Asp Val

105 110 115

gct ttc cca cgt ttg aat gct ttc ggc ttc tgg atc acc acc gtc ggt 499

Ala Phe Pro Arg Leu Asn Ala Phe Gly Phe Trp Ile Thr Thr Val Gly

120 125 130

ggt gtc gcg atg ctg acc ggc ttc ctg acc ccg ggt ggt gct gcc gac 547

Gly Val Ala Met Leu Thr Gly Phe Leu Thr Pro Gly Gly Ala Ala Asp

135 140 145

ttc ggt tgg acc atg tac tcc cca ctg tct gac gca att cac tcc cca 595

Phe Gly Trp Thr Met Tyr Ser Pro Leu Ser Asp Ala Ile His Ser Pro

150 155 160 165

ggc ctt ggc tct gac atg tgg att gtc ggt gtc ggt gca act ggt att 643

Gly Leu Gly Ser Asp Met Trp Ile Val Gly Val Gly Ala Thr Gly Ile

170 175 180

ggc tcc gtt gct tcc gca att aac atg ctc acc acc atc ctc tgc ctc 691

Gly Ser Val Ala Ser Ala Ile Asn Met Leu Thr Thr Ile Leu Cys Leu

185 190 195

cgc gca cct ggt atg acc atg ttc cgt atg cct att ttc acc tgg aat 739

Arg Ala Pro Gly Met Thr Met Phe Arg Met Pro Ile Phe Thr Trp Asn

200 205 210

atc ttc gtt gtt tcc gtt ctt gct ctg ctg atc ttc cca ctg ctg ctc 787

Ile Phe Val Val Ser Val Leu Ala Leu Leu Ile Phe Pro Leu Leu Leu

215 220 225

gct gct gca ctg ggt gtt ctg tat gac cgc aag ctt ggt gga cac ctg 835

Ala Ala Ala Leu Gly Val Leu Tyr Asp Arg Lys Leu Gly Gly His Leu

230 235 240 245

tac gat cca gct aac ggc ggc tcc ctc ctg tgg cag cac ctg ttc tgg 883

Tyr Asp Pro Ala Asn Gly Gly Ser Leu Leu Trp Gln His Leu Phe Trp

250 255 260

ttc ttc gga cac cct gag gtt tac gtt ctg gcg ctg ccg ttc ttc ggc 931

Phe Phe Gly His Pro Glu Val Tyr Val Leu Ala Leu Pro Phe Phe Gly

265 270 275

att gtt tct gag atc att cct gtg ttc tcc cgt aag cca atg ttc ggt 979

Ile Val Ser Glu Ile Ile Pro Val Phe Ser Arg Lys Pro Met Phe Gly

280 285 290

tac gtc ggc ctg atc ttc gca acc ttg tcc att ggt gca ctg tcc atg 1027

Tyr Val Gly Leu Ile Phe Ala Thr Leu Ser Ile Gly Ala Leu Ser Met

295 300 305

gct gtg tgg gct cac cac atg ttc gtt act ggc gca gtt ttg ctt ccg 1075

Ala Val Trp Ala His His Met Phe Val Thr Gly Ala Val Leu Leu Pro

310 315 320 325

ttc ttc tcc ttc atg acg ttc ctg att tcg gtt cct acc ggc gtt aag 1123

Phe Phe Ser Phe Met Thr Phe Leu Ile Ser Val Pro Thr Gly Val Lys

330 335 340

ttc ttc aac tgg gtt gga acc atg tgg aag ggt cac atc act tgg gaa 1171

Phe Phe Asn Trp Val Gly Thr Met Trp Lys Gly His Ile Thr Trp Glu

345 350 355

acc cca atg atc tgg tct gtt ggc ttc atg gct acc ttc ctc ttc ggt 1219

Thr Pro Met Ile Trp Ser Val Gly Phe Met Ala Thr Phe Leu Phe Gly

360 365 370

ggt ctg acc ggc att atg ctg gcg tcc cca cca ctg gac ttc cac ttg 1267

Gly Leu Thr Gly Ile Met Leu Ala Ser Pro Pro Leu Asp Phe His Leu

375 380 385

gct gac tcc tac ttc ctg atc gcg cac ttc cac tac acc ctc ttc ggt 1315

Ala Asp Ser Tyr Phe Leu Ile Ala His Phe His Tyr Thr Leu Phe Gly

390 395 400 405

acc gtg gtg ttc gca tcg tgt gca ggc gtt tac ttc tgg ttc ccg aag 1363

Thr Val Val Phe Ala Ser Cys Ala Gly Val Tyr Phe Trp Phe Pro Lys

410 415 420

atg act ggc cgc atg atg gac gag cgt ctt ggc aag atc cac ttc tgg 1411

Met Thr Gly Arg Met Met Asp Glu Arg Leu Gly Lys Ile His Phe Trp

425 430 435

ttg acc ttc gtc ggt ttc cac gga acc ttc ctc atc cag cac tgg gtg 1459

Leu Thr Phe Val Gly Phe His Gly Thr Phe Leu Ile Gln His Trp Val

440 445 450

ggc aac atg ggt atg cca cgt cgt tac gct gac tac ctg gat tct gat 1507

Gly Asn Met Gly Met Pro Arg Arg Tyr Ala Asp Tyr Leu Asp Ser Asp

455 460 465

ggt ttc acc atc tac aac cag atc tcc acc gtg ttc tcc ttc ctg ctt 1555

Gly Phe Thr Ile Tyr Asn Gln Ile Ser Thr Val Phe Ser Phe Leu Leu

470 475 480 485

ggc ctg tct gtc att cca ttc atc tgg aac gtc ttc aag tcc tgg cgc 1603

Gly Leu Ser Val Ile Pro Phe Ile Trp Asn Val Phe Lys Ser Trp Arg

490 495 500

tac ggt gag ctc gtt acc gtt gat gat cct tgg ggt tac ggc aac tcc 1651

Tyr Gly Glu Leu Val Thr Val Asp Asp Pro Trp Gly Tyr Gly Asn Ser

505 510 515

ctg gag tgg gca acc tcc tgc cct cct cct cgc cac aac ttc gca tcc 1699

Leu Glu Trp Ala Thr Ser Cys Pro Pro Pro Arg His Asn Phe Ala Ser

520 525 530

ttg cct cgt atc cgc tcc gag cgc cct gcg ttc gag ctg cac tac ccg 1747

Leu Pro Arg Ile Arg Ser Glu Arg Pro Ala Phe Glu Leu His Tyr Pro

535 540 545

cac atg att gaa cgc atg cgc gca gag gca cac act gga cat cac gat 1795

His Met Ile Glu Arg Met Arg Ala Glu Ala His Thr Gly His His Asp

550 555 560 565

gat att aat gct cca gaa ttg ggt acc gcc cca gcc ctt gca tct gac 1843

Asp Ile Asn Ala Pro Glu Leu Gly Thr Ala Pro Ala Leu Ala Ser Asp

570 575 580

tcc agc cgc taaaagcgtc tgatttaagt cggtacctga 1882

Ser Ser Arg

<210>88

<211>584

<212>PRT

<213>谷氨酸棒状杆菌

<400>88

Met Thr Ala Val Ala Pro Arg Val Asp Gly His Val Ala Pro Gln Arg

1 5 10 15

Pro Glu Pro Thr Gly His Ala Arg Lys Gly Ser Lys Ala Trp Leu Met

20 25 30

Met Thr Thr Thr Asp His Lys Gln Leu Gly Ile Met Tyr Ile Ile Met

35 40 45

Ser Phe Ser Phe Phe Phe Leu Gly Gly Leu Met Ala Leu Leu Ile Arg

50 55 60

Ala Glu Leu Phe Thr Pro Gly Leu Gln Phe Leu Ser Asn Glu Gln Phe

65 70 75 80

Asn Gln Leu Phe Thr Met His Gly Thr Val Met Leu Leu Leu Tyr Gly

85 90 95

Thr Pro Ile Val Trp Gly Phe Ala Asn Tyr Val Leu Pro Leu Gln Ile

100 105 110

Gly Ala Pro Asp Val Ala Phe Pro Arg Leu Asn Ala Phe Gly Phe Trp

115 120 125

Ile Thr Thr Val Gly Gly Val Ala Met Leu Thr Gly Phe Leu Thr Pro

130 135 140

Gly Gly Ala Ala Asp Phe Gly Trp Thr Met Tyr Ser Pro Leu Ser Asp

145 150 155 160

Ala Ile His Ser Pro Gly Leu Gly Ser Asp Met Trp Ile Val Gly Val

165 170 175

Gly Ala Thr Gly Ile Gly Ser Val Ala Ser Ala Ile Asn Met Leu Thr

180 185 190

Thr Ile Leu Cys Leu Arg Ala Pro Gly Met Thr Met Phe Arg Met Pro

195 200 205

Ile Phe Thr Trp Asn Ile Phe Val Val Ser Val Leu Ala Leu Leu Ile

210 215 220

Phe Pro Leu Leu Leu Ala Ala Ala Leu Gly Val Leu Tyr Asp Arg Lys

225 230 235 240

Leu Gly Gly His Leu Tyr Asp Pro Ala Asn Gly Gly Ser Leu Leu Trp

245 250 255

Gln His Leu Phe Trp Phe Phe Gly His Pro Glu Val Tyr Val Leu Ala

260 265 270

Leu Pro Phe Phe Gly Ile Val Ser Glu Ile Ile Pro Val Phe Ser Arg

275 280 285

Lys Pro Met Phe Gly Tyr Val Gly Leu Ile Phe Ala Thr Leu Ser Ile

290 295 300

Gly Ala Leu Ser Met Ala Val Trp Ala His His Met Phe Val Thr Gly

305 310 315 320

Ala Val Leu Leu Pro Phe Phe Ser Phe Met Thr Phe Leu Ile Ser Val

325 330 335

Pro Thr Gly Val Lys Phe Phe Asn Trp Val Gly Thr Met Trp Lys Gly

340 345 350

His Ile Thr Trp Glu Thr Pro Met Ile Trp Ser Val Gly Phe Met Ala

355 360 365

Thr Phe Leu Phe Gly Gly Leu Thr Gly Ile Met Leu Ala Ser Pro Pro

370 375 380

Leu Asp Phe His Leu Ala Asp Ser Tyr Phe Leu Ile Ala His Phe His

385 390 395 400

Tyr Thr Leu Phe Gly Thr Val Val Phe Ala Ser Cys Ala Gly Val Tyr

405 410 415

Phe Trp Phe Pro Lys Met Thr Gly Arg Met Met Asp Glu Arg Leu Gly

420 425 430

Lys Ile His Phe Trp Leu Thr Phe Val Gly Phe His Gly Thr Phe Leu

435 440 445

Ile Gln His Trp Val Gly Asn Met Gly Met Pro Arg Arg Tyr Ala Asp

450 455 460

Tyr Leu Asp Ser Asp Gly Phe Thr Ile Tyr Asn Gln Ile Ser Thr Val

465 470 475 480

Phe Ser Phe Leu Leu Gly Leu Ser Val Ile Pro Phe Ile Trp Asn Val

485 490 495

Phe Lys Ser Trp Arg Tyr Gly Glu Leu Val Thr Val Asp Asp Pro Trp

500 505 510

Gly Tyr Gly Asn Ser Leu Glu Trp Ala Thr Ser Cys Pro Pro Pro Arg

515 520 525

His Asn Phe Ala Ser Leu Pro Arg Ile Arg Ser Glu Arg Pro Ala Phe

530 535 540

Glu Leu His Tyr Pro His Met Ile Glu Arg Met Arg Ala Glu Ala His

545 550 555 560

Thr Gly His His Asp Asp Ile Asn Ala Pro Glu Leu Gly Thr Ala Pro

565 570 575

Ala Leu Ala Ser Asp Ser Ser Arg

580

<210>89

<211>997

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(967)

<223>RXA02560

<400>89

ttggggcaag ccagctaacg catttcttgt ggaaaccgca gacattgagg ccgcccacgc 60

ggaacttcta agagcagtgg aatgaaataa tccggtgctg atg cag ggc aac tcg 115

Met Gln Gly Asn Ser

1 5

ctt aat ctg gca gac aac agc gag aga aag aag ccc atg ccg tca cca 163

Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys Pro Met Pro Ser Pro

10 15 20

gga gaa ctt tta gcc gcc cgc tac gga caa cct gca acc tgg acg cca 211

Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro Ala Thr Trp Thr Pro

25 30 35

ccg cag tgg aat gag acg ctt gat gtc att cac cag cat cga tca gtt 259

Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His Gln His Arg Ser Val

40 45 50

cgc agg tgg ttg gat aaa ccg gtt gat gat gac acc atc cgc acc att 307

Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp Thr Ile Arg Thr Ile

55 60 65

att tcc gcc gca caa tcg gct gga acc tct tcc aat aag cag gtc att 355

Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser Asn Lys Gln Val Ile

70 75 80 85

tct gtc atc gtg gtt aaa gat cct gag ctg agg aaa ggc ctc gcg ggg 403

Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg Lys Gly Leu Ala Gly

90 95 100

atc act cgc cag atg ttt ccg cac ctt gag cag gtt ccc gcg gtg ctg 451

Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln Val Pro Ala Val Leu

105 110 115

att tgg ttg att gat tat tcc cga atc agt gcg gtg gca gcc aga gaa 499

Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala Val Ala Ala Arg Glu

120 125 130

gat ctc cca aca ggg gct ctt gat tat ctc gat gag gcc gcg ttg ggg 547

Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp Glu Ala Ala Leu Gly

135 140 145

ttc ctc gac gcc gga atc gca gct caa aac gct gca att gct gcg gag 595

Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala Ala Ile Ala Ala Glu

150 155 160 165

tca ctt gga ttg gga acg ctc tat ttg ggt tcg gtg cgc aac gat gcg 643

Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser Val Arg Asn Asp Ala

170 175 180

gaa gcc gtg cac aaa ttg ctt ggc ctt cca cct gag atc gtg cct gtc 691

Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro Glu Ile Val Pro Val

185 190 195

gtg ggc ttg gaa att ggg cat gcg gat ccg tct gaa cct gcc gga att 739

Val Gly Leu Glu Ile Gly His Ala Asp Pro Ser Glu Pro Ala Gly Ile

200 205 210

aaa cct cgc ctg cca caa gaa gcc att gtt cac tgg gat acc tac acc 787

Lys Pro Arg Leu Pro Gln Glu Ala Ile Val His Trp Asp Thr Tyr Thr

215 220 225

gag aaa aac ctc gaa ctt atc gat tcc tac gac cgc gcc ctc gac act 835

Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp Arg Ala Leu Asp Thr

230 235 240 245

tac tat tct cgc tac ggc cag cac cag ctc tgg tcg aag cag acg gcg 883

Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp Ser Lys Gln Thr Ala

250 255 260

cat agg gcg gcg tcg aaa agc ttt tca aaa acc aac agg cag ttc ctt 931

His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr Asn Arg Gln Phe Leu

265 270 275

agg ggc gtg ttt gag cgc gcc ggg ttt ggg ctg aga taaaagcatg 977

Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu Arg

280 285

attatggacg cctccctccc 997

<210>90

<211>289

<212>PRT

<213>谷氨酸棒状杆菌

<400>90

Met Gln Gly Asn Ser Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys

1 5 10 15

Pro Met Pro Ser Pro Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro

20 25 30

Ala Thr Trp Thr Pro Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His

35 40 45

Gln His Arg Ser Val Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp

50 55 60

Thr Ile Arg Thr Ile Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser

65 70 75 80

Asn Lys Gln Val Ile Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg

85 90 95

Lys Gly Leu Ala Gly Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln

100 105 110

Val Pro Ala Val Leu Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala

115 120 125

Val Ala Ala Arg Glu Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp

130 135 140

Glu Ala Ala Leu Gly Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala

145 150 155 160

Ala Ile Ala Ala Glu Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser

165 170 175

Val Arg Asn Asp Ala Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro

180 185 190

Glu Ile Val Pro Val Val Gly Leu Glu Ile Gly His Ala Asp Pro Ser

195 200 205

Glu Pro Ala Gly Ile Lys Pro Arg Leu Pro Gln Glu Ala Ile Val His

210 215 220

Trp Asp Thr Tyr Thr Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp

225 230 235 240

Arg Ala Leu Asp Thr Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp

245 250 255

Ser Lys Gln Thr Ala His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr

260 265 270

Asn Arg Gln Phe Leu Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu

275 280 285

Arg

<210>91

<211>1960

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1930)

<223>RXA02591

<400>91

atgtgtccgt tgtctcacct aaagttttaa ctagttctgt atctgaaagc tacgctaggg 60

ggcgagaact ctgtcgaatg acacaaaatc tggagaagta atg act act gct gca 115

Met Thr Thr Ala Ala

1 5

atc agg ggc ctt cag ggc gag gcg ccg acc aag aat aag gaa ctg ctg 163

Ile Arg Gly Leu Gln Gly Glu Ala Pro Thr Lys Asn Lys Glu Leu Leu

10 15 20

aac tgg atc gca gac gcc gtc gag ctc ttc cag cct gag gct gtt gtg 211

Asn Trp Ile Ala Asp Ala Val Glu Leu Phe Gln Pro Glu Ala Val Val

25 30 35

ttc gtt gat gga tcc cag gct gag tgg gat cgc atg gcg gag gat ctt 259

Phe Val Asp Gly Ser Gln Ala Glu Trp Asp Arg Met Ala Glu Asp Leu

40 45 50

gtt gaa gcc ggt acc ctc atc aag ctc aac gag gaa aag cgt ccg aac 307

Val Glu Ala Gly Thr Leu Ile Lys Leu Asn Glu Glu Lys Arg Pro Asn

55 60 65

agc tac cta gct cgt tcc aac cca tct gac gtt gcg cgc gtt gag tcc 355

Ser Tyr Leu Ala Arg Ser Asn Pro Ser Asp Val Ala Arg Val Glu Ser

70 75 80 85

cgc acc ttc atc tgc tcc gag aag gaa gaa gat gct ggc cca acc aac 403

Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp Ala Gly Pro Thr Asn

90 95 100

aac tgg gct cca cca cag gca atg aag gac gaa atg tcc aag cat tac 451

Asn Trp Ala Pro Pro Gln Ala Met Lys Asp Glu Met Ser Lys His Tyr

105 110 115

gct ggt tcc atg aag ggg cgc acc atg tac gtc gtg cct ttc tgc atg 499

Ala Gly Ser Met Lys Gly Arg Thr Met Tyr Val Val Pro Phe Cys Met

120 125 130

ggt cca atc agc gat ccg gac cct aag ctt ggt gtg cag ctc act gac 547

Gly Pro Ile Ser Asp Pro Asp Pro Lys Leu Gly Val Gln Leu Thr Asp

135 140 145

tcc gag tac gtt gtc atg tcc atg cgc atc atg acc cgc atg ggt att 595

Ser Glu Tyr Val Val Met Ser Met Arg Ile Met Thr Arg Met Gly Ile

150 155 160 165

gaa gcg ctg gac aag atc ggc gcg aac ggc agc ttc gtc agg tgc ctc 643

Glu Ala Leu Asp Lys Ile Gly Ala Asn Gly Ser Phe Val Arg Cys Leu

170 175 180

cac tcc gtt ggt gct cct ttg gag cca ggc cag gaa gac gtt gca tgg 691

His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln Glu Asp Val Ala Trp

185 190 195

cct tgc aac gac acc aag tac atc acc cag ttc cca gag acc aag gaa 739

Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe Pro Glu Thr Lys Glu

200 205 210

att tgg tcc tac ggt tcc ggc tac ggc gga aac gca atc ctg gca aag 787

Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn Ala Ile Leu Ala Lys

215 220 225

aag tgc tac gca ctg cgt atc gca tct gtc atg gct cgc gaa gaa gga 835

Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met Ala Arg Glu Glu Gly

230 235 240 245

tgg atg gct gag cac atg ctc atc ctg aag ctg atc aac cca gag ggc 883

Trp Met Ala Glu His Met Leu Ile Leu Lys Leu Ile Asn Pro Glu Gly

250 255 260

aag gcg tac cac atc gca gca gca ttc cca tct gct tgt ggc aag acc 931

Lys Ala Tyr His Ile Ala Ala Ala Phe Pro Ser Ala Cys Gly Lys Thr

265 270 275

aac ctc gcc atg atc act cca acc atc cca ggc tgg acc gct cag gtt 979

Asn Leu Ala Met Ile Thr Pro Thr Ile Pro Gly Trp Thr Ala Gln Val

280 285 290

gtt ggc gac gac atc gct tgg ctg aag ctg cgc gag gac ggc ctc tac 1027

Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg Glu Asp Gly Leu Tyr

295 300 305

gca gtt aac cca gaa aat ggt ttc ttc ggt gtt gct cca ggc acc aac 1075

Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val Ala Pro Gly Thr Asn

310 315 320 325

tac gca tcc aac cca atc gcg atg aag acc atg gaa cca ggc aac acc 1123

Tyr Ala Ser Asn Pro Ile Ala Met Lys Thr Met Glu Pro Gly Asn Thr

330 335 340

ctg ttc acc aac gtg gca ctc acc gac gac ggc gac atc tgg tgg gaa 1171

Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly Asp Ile Trp Trp Glu

345 350 355

ggc atg gac ggc gac gcc cca gct cac ctc att gac tgg atg ggc aac 1219

Gly Met Asp Gly Asp Ala Pro Ala His Leu Ile Asp Trp Met Gly Asn

360 365 370

gac tgg acc cca gag tcc gac gaa aac gct gct cac cct aac tcc cgt 1267

Asp Trp Thr Pro Glu Ser Asp Glu Asn Ala Ala His Pro Asn Ser Arg

375 380 385

tac tgc gta gca atc gac cag tcc cca gca gca gca cct gag ttc aac 1315

Tyr Cys Val Ala Ile Asp Gln Ser Pro Ala Ala Ala Pro Glu Phe Asn

390 395 400 405

gac tgg gaa ggc gtc aag atc gac gca atc ctc ttc ggt gga cgt cgc 1363

Asp Trp Glu Gly Val Lys Ile Asp Ala Ile Leu Phe Gly Gly Arg Arg

410 415 420

gca gac acc gtc cca ctg gtt acc cag acc tac gac tgg gag cac ggc 1411

Ala Asp Thr Val Pro Leu Val Thr Gln Thr Tyr Asp Trp Glu His Gly

425 430 435

acc atg gtt ggt gca ctg ctc gca tcc ggt cag acc gca gct tcc gca 1459

Thr Met Val Gly Ala Leu Leu Ala Ser Gly Gln Thr Ala Ala Ser Ala

440 445 450

gaa gca aag gtc ggc aca ctc cgc cac gac cca atg gca atg ctc cca 1507

Glu Ala Lys Val Gly Thr Leu Arg His Asp Pro Met Ala Met Leu Pro

455 460 465

ttc att ggc tac aac gct ggt gaa tac ctg cag aac tgg att gac atg 1555

Phe Ile Gly Tyr Asn Ala Gly Glu Tyr Leu Gln Asn Trp Ile Asp Met

470 475 480 485

ggt aac aag ggt ggc gac aag atg cca tcc atc ttc ctg gtc aac tgg 1603

Gly Asn Lys Gly Gly Asp Lys Met Pro Ser Ile Phe Leu Val Asn Trp

490 495 500

ttc cgc cgt ggc gaa gat gga cgc ttc ctg tgg cct ggc ttc ggc gac 1651

Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp Pro Gly Phe Gly Asp

505 510 515

aac tct cgc gtt ctg aag tgg gtc atc gac cgc atc gaa ggc cac gtt 1699

Asn Ser Arg Val Leu Lys Trp Val Ile Asp Arg Ile Glu Gly His Val

520 525 530

ggc gca gac gag acc gtt gtt gga cac acc gct aag gcc gaa gac ctc 1747

Gly Ala Asp Glu Thr Val Val Gly His Thr Ala Lys Ala Glu Asp Leu

535 540 545

gac ctc gac ggc ctc gac acc cca att gag gat gtc aag gaa gca ctg 1795

Asp Leu Asp Gly Leu Asp Thr Pro Ile Glu Asp Val Lys Glu Ala Leu

550 555 560 565

acc gct cct gca gag cag tgg gca aac gac gtt gaa gac aac gcc gag 1843

Thr Ala Pro Ala Glu Gln Trp Ala Asn Asp Val Glu Asp Asn Ala Glu

570 575 580

tac ctc act ttc ctc gga cca cgt gtt cct gca gag gtt cac agc cag 1891

Tyr Leu Thr Phe Leu Gly Pro Arg Val Pro Ala Glu Val His Ser Gln

585 590 595

ttc gat gct ctg aag gcc cgc att tca gca gct cac gct taaagttcac 1940

Phe Asp Ala Leu Lys Ala Arg Ile Ser Ala Ala His Ala

600 605 610

gcttaagaac tgctaaataa 1960

<210>92

<211>610

<212>PRT

<213>谷氨酸棒状杆菌

<400>92

Met Thr Thr Ala Ala Ile Arg Gly Leu Gln Gly Glu Ala Pro Thr Lys

1 5 10 15

Asn Lys Glu Leu Leu Asn Trp Ile Ala Asp Ala Val Glu Leu Phe Gln

20 25 30

Pro Glu Ala Val Val Phe Val Asp Gly Ser Gln Ala Glu Trp Asp Arg

35 40 45

Met Ala Glu Asp Leu Val Glu Ala Gly Thr Leu Ile Lys Leu Asn Glu

50 55 60

Glu Lys Arg Pro Asn Ser Tyr Leu Ala Arg Ser Asn Pro Ser Asp Val

65 70 75 80

Ala Arg Val Glu Ser Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp

85 90 95

Ala Gly Pro Thr Asn Asn Trp Ala Pro Pro Gln Ala Met Lys Asp Glu

100 105 110

Met Ser Lys His Tyr Ala Gly Ser Met Lys Gly Arg Thr Met Tyr Val

115 120 125

Val Pro Phe Cys Met Gly Pro Ile Ser Asp Pro Asp Pro Lys Leu Gly

130 135 140

Val Gln Leu Thr Asp Ser Glu Tyr Val Val Met Ser Met Arg Ile Met

145 150 155 160

Thr Arg Met Gly Ile Glu Ala Leu Asp Lys Ile Gly Ala Asn Gly Ser

165 170 175

Phe Val Arg Cys Leu His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln

180 185 190

Glu Asp Val Ala Trp Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe

195 200 205

Pro Glu Thr Lys Glu Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn

210 215 220

Ala Ile Leu Ala Lys Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met

225 230 235 240

Ala Arg Glu Glu Gly Trp Met Ala Glu His Met Leu Ile Leu Lys Leu

245 250 255

Ile Asn Pro Glu Gly Lys Ala Tyr His Ile Ala Ala Ala Phe Pro Ser

260 265 270

Ala Cys Gly Lys Thr Asn Leu Ala Met Ile Thr Pro Thr Ile Pro Gly

275 280 285

Trp Thr Ala Gln Val Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg

290 295 300

Glu Asp Gly Leu Tyr Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val

305 310 315 320

Ala Pro Gly Thr Asn Tyr Ala Ser Asn Pro Ile Ala Met Lys Thr Met

325 330 335

Glu Pro Gly Asn Thr Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly

340 345 350

Asp Ile Trp Trp Glu Gly Met Asp Gly Asp Ala Pro Ala His Leu Ile

355 360 365

Asp Trp Met Gly Asn Asp Trp Thr Pro Glu Ser Asp Glu Asn Ala Ala

370 375 380

His Pro Asn Ser Arg Tyr Cys Val Ala Ile Asp Gln Ser Pro Ala Ala

385 390 395 400

Ala Pro Glu Phe Asn Asp Trp Glu Gly Val Lys Ile Asp Ala Ile Leu

405 410 415

Phe Gly Gly Arg Arg Ala Asp Thr Val Pro Leu Val Thr Gln Thr Tyr

420 425 430

Asp Trp Glu His Gly Thr Met Val Gly Ala Leu Leu Ala Ser Gly Gln

435 440 445

Thr Ala Ala Ser Ala Glu Ala Lys Val Gly Thr Leu Arg His Asp Pro

450 455 460

Met Ala Met Leu Pro Phe Ile Gly Tyr Asn Ala Gly Glu Tyr Leu Gln

465 470 475 480

Asn Trp Ile Asp Met Gly Asn Lys Gly Gly Asp Lys Met Pro Ser Ile

485 490 495

Phe Leu Val Asn Trp Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp

500 505 510

Pro Gly Phe Gly Asp Asn Ser Arg Val Leu Lys Trp Val Ile Asp Arg

515 520 525

Ile Glu Gly His Val Gly Ala Asp Glu Thr Val Val Gly His Thr Ala

530 535 540

Lys Ala Glu Asp Leu Asp Leu Asp Gly Leu Asp Thr Pro Ile Glu Asp

545 550 555 560

Val Lys Glu Ala Leu Thr Ala Pro Ala Glu Gln Trp Ala Asn Asp Val

565 570 575

Glu Asp Asn Ala Glu Tyr Leu Thr Phe Leu Gly Pro Arg Val Pro Ala

580 585 590

Glu Val His Ser Gln Phe Asp Ala Leu Lys Ala Arg Ile Ser Ala Ala

595 600 605

His Ala

610

<210>93

<211>1015

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(985)

<223>RXA02654

<400>93

tattttcgga aatttataca gcaatcctcg aaatcctaat aaagatccct tatcgtggga 60

gaggtacggt agttcgttcg aggacaacgt cgagaaaggc atg att tca ttg cta 115

Met Ile Ser Leu Leu

1 5

aat gat cca cgt acg cta ttc ccg aaa gtc gat ccc cca aag caa agc 163

Asn Asp Pro Arg Thr Leu Phe Pro Lys Val Asp Pro Pro Lys Gln Ser

10 15 20

cag ccg gaa cca ggc cta gat ata aaa ctt tcc ccc caa gcc gat att 211

Gln Pro Glu Pro Gly Leu Asp Ile Lys Leu Ser Pro Gln Ala Asp Ile

25 30 35

ggt ctc tcc agc tat caa gga agt gga agg ctt aag ggc cgc aag gct 259

Gly Leu Ser Ser Tyr Gln Gly Ser Gly Arg Leu Lys Gly Arg Lys Ala

40 45 50

ctt att act ggt ggc gat tct ggg att gga gct gcc gta gca atc gct 307

Leu Ile Thr Gly Gly Asp Ser Gly Ile Gly Ala Ala Val Ala Ile Ala

55 60 65

tat gct cgc gag ggg gca gat gtt gcg atc gct tac ttg ccc gaa gaa 355

Tyr Ala Arg Glu Gly Ala Asp Val Ala Ile Ala Tyr Leu Pro Glu Glu

70 75 80 85

caa gcc gat gct gac aga gtg ctc caa gca atc gag gaa aca ggt caa 403

Gln Ala Asp Ala Asp Arg Val Leu Gln Ala Ile Glu Glu Thr Gly Gln

90 95 100

aaa gct ttt tct ttc cct ggt gat ctc cgt gat cca gaa tac tgt cgc 451

Lys Ala Phe Ser Phe Pro Gly Asp Leu Arg Asp Pro Glu Tyr Cys Arg

105 110 115

tcg ctg gtc caa gag acg gtg aac gct tta ggt ggc cta gac atc ttg 499

Ser Leu Val Gln Glu Thr Val Asn Ala Leu Gly Gly Leu Asp Ile Leu

120 125 130

gtc aac aac gcg tca cgt cag gtg tgg gca cct ggt ttg acc gaa att 547

Val Asn Asn Ala Ser Arg Gln Val Trp Ala Pro Gly Leu Thr Glu Ile

135 140 145

acc gac gaa aac ttc gac cag act ttg cag gtt aac ctc tat ggt agt 595

Thr Asp Glu Asn Phe Asp Gln Thr Leu Gln Val Asn Leu Tyr Gly Ser

150 155 160 165

ttt cgg gtt acc aaa gca gct ata cct cat ctg aag ccc gga tca tcg 643

Phe Arg Val Thr Lys Ala Ala Ile Pro His Leu Lys Pro Gly Ser Ser

170 175 180

ata atc ttt aca tcg tcc att cag gcg tac caa cct tcg gaa acc ctc 691

Ile Ile Phe Thr Ser Ser Ile Gln Ala Tyr Gln Pro Ser Glu Thr Leu

185 190 195

ttg gat tac gcc atg act aag gcg gca ttg aac aat ttg tca aag ggc 739

Leu Asp Tyr Ala Met Thr Lys Ala Ala Leu Asn Asn Leu Ser Lys Gly

200 205 210

ttg gca agt agt ctg ata ggc gat ggc att cgg gta aat tct gta gcc 787

Leu Ala Ser Ser Leu Ile Gly Asp Gly Ile Arg Val Asn Ser Val Ala

215 220 225

cca ggt cct ttc tgg acg ccg ttg caa ccc agc cat ggt cag cca caa 835

Pro Gly Pro Phe Trp Thr Pro Leu Gln Pro Ser His Gly Gln Pro Gln

230 235 240 245

gag aaa ata gaa gga ttt ggc cag cac gct ccg att gga aga gcg ggt 883

Glu Lys Ile Glu Gly Phe Gly Gln His Ala Pro Ile Gly Arg Ala Gly

250 255 260

cac cct gtt gag ttg gca ggt gcg tac gtt ttt ctc gct tct gac gaa 931

His Pro Val Glu Leu Ala Gly Ala Tyr Val Phe Leu Ala Ser Asp Glu

265 270 275

gcc agc tat gtg gta gga gaa acc ctg gga gtc aca ggt ggg acg ccc 979

Ala Ser Tyr Val Val Gly Glu Thr Leu Gly Val Thr Gly Gly Thr Pro

280 285 290

acc cca tagtcggtac aagcggaatc actttctcag 1015

Thr Pro

295

<210>94

<211>295

<212>PRT

<213>谷氨酸棒状杆菌

<400>94

Met Ile Ser Leu Leu Asn Asp Pro Arg Thr Leu Phe Pro Lys Val Asp

1 5 10 15

Pro Pro Lys Gln Ser Gln Pro Glu Pro Gly Leu Asp Ile Lys Leu Ser

20 25 30

Pro Gln Ala Asp Ile Gly Leu Ser Ser Tyr Gln Gly Ser Gly Arg Leu

35 40 45

Lys Gly Arg Lys Ala Leu Ile Thr Gly Gly Asp Ser Gly Ile Gly Ala

50 55 60

Ala Val Ala Ile Ala Tyr Ala Arg Glu Gly Ala Asp Val Ala Ile Ala

65 70 75 80

Tyr Leu Pro Glu Glu Gln Ala Asp Ala Asp Arg Val Leu Gln Ala Ile

85 90 95

Glu Glu Thr Gly Gln Lys Ala Phe Ser Phe Pro Gly Asp Leu Arg Asp

100 105 110

Pro Glu Tyr Cys Arg Ser Leu Val Gln Glu Thr Val Asn Ala Leu Gly

115 120 125

Gly Leu Asp Ile Leu Val Asn Asn Ala Ser Arg Gln Val Trp Ala Pro

130 135 140

Gly Leu Thr Glu Ile Thr Asp Glu Asn Phe Asp Gln Thr Leu Gln Val

145 150 155 160

Asn Leu Tyr Gly Ser Phe Arg Val Thr Lys Ala Ala Ile Pro His Leu

165 170 175

Lys Pro Gly Ser Ser Ile Ile Phe Thr Ser Ser Ile Gln Ala Tyr Gln

180 185 190

Pro Ser Glu Thr Leu Leu Asp Tyr Ala Met Thr Lys Ala Ala Leu Asn

195 200 205

Asn Leu Ser Lys Gly Leu Ala Ser Ser Leu Ile Gly Asp Gly Ile Arg

210 215 220

Val Asn Ser Val Ala Pro Gly Pro Phe Trp Thr Pro Leu Gln Pro Ser

225 230 235 240

His Gly Gln Pro Gln Glu Lys Ile Glu Gly Phe Gly Gln His Ala Pro

245 250 255

Ile Gly Arg Ala Gly His Pro Val Glu Leu Ala Gly Ala Tyr Val Phe

260 265 270

Leu Ala Ser Asp Glu Ala Ser Tyr Val Val Gly Glu Thr Leu Gly Val

275 280 285

Thr Gly Gly Thr Pro Thr Pro

290 295

<210>95

<211>1084

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1054)

<223>RXA02694

<400>95

taatcgggca taattaaagg tgtaacaaag gaatccgggc acaagctctt gctgattttc 60

tgagctgctt tgtgggttgt ccggttaggg aaatcaggaa gtg gga tcg aaa atg 115

Val Gly Ser Lys Met

1 5

aaa gaa acc gtc ggt aac aag att gtc ctc att ggc gca gga gat gtt 163

Lys Glu Thr Val Gly Asn Lys Ile Val Leu Ile Gly Ala Gly Asp Val

10 15 20

gga gtt gca tac gca tac gca ctg atc aac cag ggc atg gca gat cac 211

Gly Val Ala Tyr Ala Tyr Ala Leu Ile Asn Gln Gly Met Ala Asp His

25 30 35

ctt gcg atc atc gac atc gat gaa aag aaa ctc gaa ggc aac gtc atg 259

Leu Ala Ile Ile Asp Ile Asp Glu Lys Lys Leu Glu Gly Asn Val Met

40 45 50

gac tta aac cat ggt gtt gtg tgg gcc gat tcc cgc acc cgc gtc acc 307

Asp Leu Asn His Gly Val Val Trp Ala Asp Ser Arg Thr Arg Val Thr

55 60 65

aag ggc acc tac gct gac tgc gaa gac gca gcc atg gtt gtc att tgt 355

Lys Gly Thr Tyr Ala Asp Cys Glu Asp Ala Ala Met Val Val Ile Cys

70 75 80 85

gcc ggc gca gcc caa aag cca ggc gag acc cgc ctc cag ctg gtg gac 403

Ala Gly Ala Ala Gln Lys Pro Gly Glu Thr Arg Leu Gln Leu Val Asp

90 95 100

aaa aac gtc aag att atg aaa tcc atc gtc ggc gat gtc atg gac agc 451

Lys Asn Val Lys Ile Met Lys Ser Ile Val Gly Asp Val Met Asp Ser

105 110 115

gga ttc gac ggc atc ttc ctc gtg gcg tcc aac cca gtg gat atc ctg 499

Gly Phe Asp Gly Ile Phe Leu Val Ala Ser Asn Pro Val Asp Ile Leu

120 125 130

acc tac gca gtg tgg aaa ttc tcc ggc ttg gaa tgg aac cgc gtg atc 547

Thr Tyr Ala Val Trp Lys Phe Ser Gly Leu Glu Trp Asn Arg Val Ile

135 140 145

ggc tcc gga act gtc ctg gac tcc gct cga ttc cgc tac atg ctg ggc 595

Gly Ser Gly Thr Val Leu Asp Ser Ala Arg Phe Arg Tyr Met Leu Gly

150 155 160 165

gaa ctc tac gaa gtg gca cca agc tcc gtc cac gcc tac atc atc ggc 643

Glu Leu Tyr Glu Val Ala Pro Ser Ser Val His Ala Tyr Ile Ile Gly

170 175 180

gaa cac ggc gac act gaa ctt cca gtc ctg tcc tcc gcg acc atc gca 691

Glu His Gly Asp Thr Glu Leu Pro Val Leu Ser Ser Ala Thr Ile Ala

185 190 195

ggc gta tcg ctt agc cga atg ctg gac aaa gac cca gag ctt gag ggc 739

Gly Val Ser Leu Ser Arg Met Leu Asp Lys Asp Pro Glu Leu Glu Gly

200 205 210

cgt cta gag aaa att ttc gaa gac acc cgc gac gct gcc tat cac att 787

Arg Leu Glu Lys Ile Phe Glu Asp Thr Arg Asp Ala Ala Tyr His Ile

215 220 225

atc gac gcc aag ggc tcc act tcc tac ggc atc ggc atg ggt ctt gct 835

Ile Asp Ala Lys Gly Ser Thr Ser Tyr Gly Ile Gly Met Gly Leu Ala

230 235 240 245

cgc atc acc cgc gca atc ctg cag aac caa gac gtt gca gtc cca gtc 883

Arg Ile Thr Arg Ala Ile Leu Gln Asn Gln Asp Val Ala Val Pro Val

250 255 260

tct gca ctg ctc cac ggt gaa tac ggt gag gaa gac atc tac atc ggc 931

Ser Ala Leu Leu His Gly Glu Tyr Gly Glu Glu Asp Ile Tyr Ile Gly

265 270 275

acc cca gct gtg gtg aac cgc cga ggc atc cgc cgc gtt gtc gaa cta 979

Thr Pro Ala Val Val Asn Arg Arg Gly Ile Arg Arg Val Val Glu Leu

280 285 290

gaa atc acc gac cac gag atg gaa cgc ttc aag cat tcc gca aat acc 1027

Glu Ile Thr Asp His Glu Met Glu Arg Phe Lys His Ser Ala Asn Thr

295 300 305

ctg cgc gaa att cag aag cag ttc ttc taaatctttg gcgcctagtt 1074

Leu Arg Glu Ile Gln Lys Gln Phe Phe

310 315

ggcgacgcaa 1084

<210>96

<211>318

<212>PRT

<213>谷氨酸棒状杆菌

<400>96

Val Gly Ser Lys Met Lys Glu Thr Val Gly Asn Lys Ile Val Leu Ile

1 5 10 15

Gly Ala Gly Asp Val Gly Val Ala Tyr Ala Tyr Ala Leu Ile Asn Gln

20 25 30

Gly Met Ala Asp His Leu Ala Ile Ile Asp Ile Asp Glu Lys Lys Leu

35 40 45

Glu Gly Asn Val Met Asp Leu Asn His Gly Val Val Trp Ala Asp Ser

50 55 60

Arg Thr Arg Val Thr Lys Gly Thr Tyr Ala Asp Cys Glu Asp Ala Ala

65 70 75 80

Met Val Val Ile Cys Ala Gly Ala Ala Gln Lys Pro Gly Glu Thr Arg

85 90 95

Leu Gln Leu Val Asp Lys Asn Val Lys Ile Met Lys Ser Ile Val Gly

100 105 110

Asp Val Met Asp Ser Gly Phe Asp Gly Ile Phe Leu Val Ala Ser Asn

115 120 125

Pro Val Asp Ile Leu Thr Tyr Ala Val Trp Lys Phe Ser Gly Leu Glu

130 135 140

Trp Asn Arg Val Ile Gly Ser Gly Thr Val Leu Asp Ser Ala Arg Phe

145 150 155 160

Arg Tyr Met Leu Gly Glu Leu Tyr Glu Val Ala Pro Ser Ser Val His

165 170 175

Ala Tyr Ile Ile Gly Glu His Gly Asp Thr Glu Leu Pro Val Leu Ser

180 185 190

Ser Ala Thr Ile Ala Gly Val Ser Leu Ser Arg Met Leu Asp Lys Asp

195 200 205

Pro Glu Leu Glu Gly Arg Leu Glu Lys Ile Phe Glu Asp Thr Arg Asp

210 215 220

Ala Ala Tyr His Ile Ile Asp Ala Lys Gly Ser Thr Ser Tyr Gly Ile

225 230 235 240

Gly Met Gly Leu Ala Arg Ile Thr Arg Ala Ile Leu Gln Asn Gln Asp

245 250 255

Val Ala Val Pro Val Ser Ala Leu Leu His Gly Glu Tyr Gly Glu Glu

260 265 270

Asp Ile Tyr Ile Gly Thr Pro Ala Val Val Asn Arg Arg Gly Ile Arg

275 280 285

Arg Val Val Glu Leu Glu Ile Thr Asp His Glu Met Glu Arg Phe Lys

290 295 300

His Ser Ala Asn Thr Leu Arg Glu Ile Gln Lys Gln Phe Phe

305 310 315

<210>97

<211>955

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(925)

<223>RXA02735

<400>97

accggctcga tcatcatcac catctatgac gctcataccc ttcaggtaga gatgccggaa 60

tccggcaatg ccccatcgct ggtggctatt ggtcgtcgaa gtg agt ccg act gct 115

Val Ser Pro Thr Ala

1 5

tgt ctg agg agc ttc gcc aca tgg atc cag att tgg gct acc agc acg 163

Cys Leu Arg Ser Phe Ala Thr Trp Ile Gln Ile Trp Ala Thr Ser Thr

10 15 20

cac tat ccg gct tgt cca gcg tca agc tgg aaa ccg tct aag gag aaa 211

His Tyr Pro Ala Cys Pro Ala Ser Ser Trp Lys Pro Ser Lys Glu Lys

25 30 35

tac aac act atg gtt gat gta gta cgc gca cgc gat act gaa gat ttg 259

Tyr Asn Thr Met Val Asp Val Val Arg Ala Arg Asp Thr Glu Asp Leu

40 45 50

gtt gca cag gct gcc tcc aaa ttc att gag gtt gtt gaa gca gca act 307

Val Ala Gln Ala Ala Ser Lys Phe Ile Glu Val Val Glu Ala Ala Thr

55 60 65

gcc aat aat ggc acc gca cag gta gtg ctc acc ggt ggt ggc gcc ggc 355

Ala Asn Asn Gly Thr Ala Gln Val Val Leu Thr Gly Gly Gly Ala Gly

70 75 80 85

atc aag ttg ctg gaa aag ctc agc gtt gat gcg gct gac ctt gcc tgg 403

Ile Lys Leu Leu Glu Lys Leu Ser Val Asp Ala Ala Asp Leu Ala Trp

90 95 100

gat cgc att cat gtg ttc ttc ggc gat gag cgc aat gtc cct gtc agt 451

Asp Arg Ile His Val Phe Phe Gly Asp Glu Arg Asn Val Pro Val Ser

105 110 115

gat tct gag tcc aat gag ggc cag gct cgt gag gca ctg ttg tcc aag 499

Asp Ser Glu Ser Asn Glu Gly Gln Ala Arg Glu Ala Leu Leu Ser Lys

120 125 130

gtt tct atc cct gaa gcc aac att cac gga tat ggt ctc ggc gac gta 547

Val Ser Ile Pro Glu Ala Asn Ile His Gly Tyr Gly Leu Gly Asp Val

135 140 145

gat ctt gca gag gca gcc cgc gct tac gaa gct gtg ttg gat gaa ttc 595

Asp Leu Ala Glu Ala Ala Arg Ala Tyr Glu Ala Val Leu Asp Glu Phe

150 155 160 165

gca cca aac ggc ttt gat ctt cac ctg ctc ggc atg ggt ggc gaa ggc 643

Ala Pro Asn Gly Phe Asp Leu His Leu Leu Gly Met Gly Gly Glu Gly

170 175 180

cat atc aac tcc ctg ttc cct cac acc gat gca gtc aag gaa tcc tcc 691

His Ile Asn Ser Leu Phe Pro His Thr Asp Ala Val Lys Glu Ser Ser

185 190 195

gca aag gtc atc gcg gtg ttt gat tcc cct aag cct cct tca gag cgt 739

Ala Lys Val Ile Ala Val Phe Asp Ser Pro Lys Pro Pro Ser Glu Arg

200 205 210

gca act cta acc ctt cct gcg gtt cac tcc gca aag cgc gtg tgg ttg 787

Ala Thr Leu Thr Leu Pro Ala Val His Ser Ala Lys Arg Val Trp Leu

215 220 225

ctg gtt tct ggt gcg gag aag gct gag gca gct gcg gcg atc gtc aac 835

Leu Val Ser Gly Ala Glu Lys Ala Glu Ala Ala Ala Ala Ile Val Asn

230 235 240 245

ggt gag cct gct gtt gag tgg cct gct gct gga gct acc gga tct gag 883

Gly Glu Pro Ala Val Glu Trp Pro Ala Ala Gly Ala Thr Gly Ser Glu

250 255 260

gaa acg gta ttg ttc ttg gct gat gat gct gca gga aat ctc 925

Glu Thr Val Leu Phe Leu Ala Asp Asp Ala Ala Gly Asn Leu

265 270 275

taagcagcgc cagctctaac aagaagcttt 955

<210>98

<211>275

<212>PRT

<213>谷氨酸棒状杆菌

<400>98

Val Ser Pro Thr Ala Cys Leu Arg Ser Phe Ala Thr Trp Ile Gln Ile

1 5 10 15

Trp Ala Thr Ser Thr His Tyr Pro Ala Cys Pro Ala Ser Ser Trp Lys

20 25 30

Pro Ser Lys Glu Lys Tyr Asn Thr Met Val Asp Val Val Arg Ala Arg

35 40 45

Asp Thr Glu Asp Leu Val Ala Gln Ala Ala Ser Lys Phe Ile Glu Val

50 55 60

Val Glu Ala Ala Thr Ala Asn Asn Gly Thr Ala Gln Val Val Leu Thr

65 70 75 80

Gly Gly Gly Ala Gly Ile Lys Leu Leu Glu Lys Leu Ser Val Asp Ala

85 90 95

Ala Asp Leu Ala Trp Asp Arg Ile His Val Phe Phe Gly Asp Glu Arg

100 105 110

Asn Val Pro Val Ser Asp Ser Glu Ser Asn Glu Gly Gln Ala Arg Glu

115 120 125

Ala Leu Leu Ser Lys Val Ser Ile Pro Glu Ala Asn Ile His Gly Tyr

130 135 140

Gly Leu Gly Asp Val Asp Leu Ala Glu Ala Ala Arg Ala Tyr Glu Ala

145 150 155 160

Val Leu Asp Glu Phe Ala Pro Asn Gly Phe Asp Leu His Leu Leu Gly

165 170 175

Met Gly Gly Glu Gly His Ile Asn Ser Leu Phe Pro His Thr Asp Ala

180 185 190

Val Lys Glu Ser Ser Ala Lys Val Ile Ala Val Phe Asp Ser Pro Lys

195 200 205

Pro Pro Ser Glu Arg Ala Thr Leu Thr Leu Pro Ala Val His Ser Ala

210 215 220

Lys Arg Val Trp Leu Leu Val Ser Gly Ala Glu Lys Ala Glu Ala Ala

225 230 235 240

Ala Ala Ile Val Asn Gly Glu Pro Ala Val Glu Trp Pro Ala Ala Gly

245 250 255

Ala Thr Gly Ser Glu Glu Thr Val Leu Phe Leu Ala Asp Asp Ala Ala

260 265 270

Gly Asn Leu

275

<210>99

<211>2230

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2200)

<223>RXA02739

<400>99

cctttgccaa atttgaacca attaacctaa gtcgtagatc tgatcatcgg atctaacgaa 60

aacgaaccaa aactttggtc ccggtttaac ccaggaagga ttg acc acc ttg acg 115

Leu Thr Thr Leu Thr

1 5

ctg tca cct gaa ctt cag gcg ctc act gta cgc aat tac ccc tct gat 163

Leu Ser Pro Glu Leu Gln Ala Leu Thr Val Arg Asn Tyr Pro Ser Asp

10 15 20

tgg tcc gat gtg gac acc aag gct gta gac act gtt cgt gtc ctc gct 211

Trp Ser Asp Val Asp Thr Lys Ala Val Asp Thr Val Arg Val Leu Ala

25 30 35

gca gac gct gta gaa aac tgt ggc tcc ggc cac cca ggc acc gca atg 259

Ala Asp Ala Val Glu Asn Cys Gly Ser Gly His Pro Gly Thr Ala Met

40 45 50

agc ctg gct ccc ctt gca tac acc ttg tac cag cgg gtt atg aac gta 307

Ser Leu Ala Pro Leu Ala Tyr Thr Leu Tyr Gln Arg Val Met Asn Val

55 60 65

gat cca cag gac acc aac tgg gca ggc cgt gac cgc ttc gtt ctt tct 355

Asp Pro Gln Asp Thr Asn Trp Ala Gly Arg Asp Arg Phe Val Leu Ser

70 75 80 85

tgt ggc cac tcc tct ttg acc cag tac atc cag ctt tac ttg ggt gga 403

Cys Gly His Ser Ser Leu Thr Gln Tyr Ile Gln Leu Tyr Leu Gly Gly

90 95 100

ttc ggc ctt gag atg gat gac ctg aag gct ctg cgc acc tgg gat tcc 451

Phe Gly Leu Glu Met Asp Asp Leu Lys Ala Leu Arg Thr Trp Asp Ser

105 110 115

ttg acc cca gga cac cct gag tac cgc cac acc aag ggc gtt gag atc 499

Leu Thr Pro Gly His Pro Glu Tyr Arg His Thr Lys Gly Val Glu Ile

120 125 130

acc act ggc cct ctt ggc cag ggt ctt gca tct gca gtt ggt atg gcc 547

Thr Thr Gly Pro Leu Gly Gln Gly Leu Ala Ser Ala Val Gly Met Ala

135 140 145

atg gct gct cgt cgt gag cgt ggc cta ttc gac cca acc gct gct gag 595

Met Ala Ala Arg Arg Glu Arg Gly Leu Phe Asp Pro Thr Ala Ala Glu

150 155 160 165

ggc gaa tcc cca ttc gac cac cac atc tac gtc att gct tct gat ggt 643

Gly Glu Ser Pro Phe Asp His His Ile Tyr Val Ile Ala Ser Asp Gly

170 175 180

gac ctg cag gaa ggt gtc acc tct gag gca tcc tcc atc gct ggc acc 691

Asp Leu Gln Glu Gly Val Thr Ser Glu Ala Ser Ser Ile Ala Gly Thr

185 190 195

cag cag ctg ggc aac ctc atc gtg ttc tgg gat gac aac cgc atc tcc 739

Gln Gln Leu Gly Asn Leu Ile Val Phe Trp Asp Asp Asn Arg Ile Ser

200 205 210

atc gaa gac aac act gag atc gct ttc aac gag gac gtt gtt gct cgt 787

Ile Glu Asp Asn Thr Glu Ile Ala Phe Asn Glu Asp Val Val Ala Arg

215 220 225

tac aag gct tac ggc tgg cag acc att gag gtt gag gct ggc gag gac 835

Tyr Lys Ala Tyr Gly Trp Gln Thr Ile Glu Val Glu Ala Gly Glu Asp

230 235 240 245

gtt gca gca atc gaa gct gca gtg gct gag gct aag aag gac acc aag 883

Val Ala Ala Ile Glu Ala Ala Val Ala Glu Ala Lys Lys Asp Thr Lys

250 255 260

cga cct acc ttc atc cgc gtt cgc acc atc atc ggc ttc cca gct cca 931

Arg Pro Thr Phe Ile Arg Val Arg Thr Ile Ile Gly Phe Pro Ala Pro

265 270 275

act atg atg aac acc ggt gct gtg cac ggt gct gct ctt ggc gca gct 979

Thr Met Met Asn Thr Gly Ala Val His Gly Ala Ala Leu Gly Ala Ala

280 285 290

gag gtt gca gca acc aag act gag ctt gga ttc gat cct gag gct cac 1027

Glu Val Ala Ala Thr Lys Thr Glu Leu Gly Phe Asp Pro Glu Ala His

295 300 305

ttc gcg atc gac gat gag gtt atc gct cac acc cgc tcc ctc gca gag 1075

Phe Ala Ile Asp Asp Glu Val Ile Ala His Thr Arg Ser Leu Ala Glu

310 315 320 325

cgc gct gca cag aag aag gct gca tgg cag gtc aag ttc gat gag tgg 1123

Arg Ala Ala Gln Lys Lys Ala Ala Trp Gln Val Lys Phe Asp Glu Trp

330 335 340

gca gct gcc aac cct gag aac aag gct ctg ttc gat cgc ctg aac tcc 1171

Ala Ala Ala Asn Pro Glu Asn Lys Ala Leu Phe Asp Arg Leu Asn Ser

345 350 355

cgt gag ctt cca gcg ggc tac gct gac gag ctc cca aca tgg gat gca 1219

Arg Glu Leu Pro Ala Gly Tyr Ala Asp Glu Leu Pro Thr Trp Asp Ala

360 365 370

gat gag aag ggc gtc gca act cgt aag gct tcc gag gct gca ctt cag 1267

Asp Glu Lys Gly Val Ala Thr Arg Lys Ala Ser Glu Ala Ala Leu Gln

375 380 385

gca ctg ggc aag acc ctt cct gag ctg tgg ggc ggt tcc gct gac ctc 1315

Ala Leu Gly Lys Thr Leu Pro Glu Leu Trp Gly Gly Ser Ala Asp Leu

390 395 400 405

gca ggt tcc aac aac acc gtg atc aag ggc tcc cct tcc ttc ggc cct 1363

Ala Gly Ser Asn Asn Thr Val Ile Lys Gly Ser Pro Ser Phe Gly Pro

410 415 420

gag tcc atc tcc acc gag acc tgg tct gct gag cct tac ggc cgt aac 1411

Glu Ser Ile Ser Thr Glu Thr Trp Ser Ala Glu Pro Tyr Gly Arg Asn

425 430 435

ctg cac ttc ggt atc cgt gag cac gct atg gga tcc atc ctc aac ggc 1459

Leu His Phe Gly Ile Arg Glu His Ala Met Gly Ser Ile Leu Asn Gly

440 445 450

att tcc ctc cac ggt ggc acc cgc cca tac ggc gga acc ttc ctc atc 1507

Ile Ser Leu His Gly Gly Thr Arg Pro Tyr Gly Gly Thr Phe Leu Ile

455 460 465

ttc tcc gac tac atg cgt cct gca gtt cgt ctt gca gct ctc atg gag 1555

Phe Ser Asp Tyr Met Arg Pro Ala Val Arg Leu Ala Ala Leu Met Glu

470 475 480 485

acc gac gct tac tac gtc tgg acc cac gac tcc atc ggt ctg ggc gaa 1603

Thr Asp Ala Tyr Tyr Val Trp Thr His Asp Ser Ile Gly Leu Gly Glu

490 495 500

gat ggc cca acc cac cag cct gtt gaa acc ttg gct gca ctg cgc gcc 1651

Asp Gly Pro Thr His Gln Pro Val Glu Thr Leu Ala Ala Leu Arg Ala

505 510 515

atc cca ggt ctg tcc gtc ctg cgt cct gca gat gcg aac gag acc gcc 1699

Ile Pro Gly Leu Ser Val Leu Arg Pro Ala Asp Ala Asn Glu Thr Ala

520 525 530

cag gct tgg gct gca gca ctt gag tac aag gaa ggc cct aag ggt ctt 1747

Gln Ala Trp Ala Ala Ala Leu Glu Tyr Lys Glu Gly Pro Lys Gly Leu

535 540 545

gca ctg acc cgc cag aac gtt cct gtt ctg gaa ggc acc aag gag aag 1795

Ala Leu Thr Arg Gln Asn Val Pro Val Leu Glu Gly Thr Lys Glu Lys

550 555 560 565

gct gct gaa ggc gtt cgc cgc ggt ggc tac gtc ctg gtt gag ggt tcc 1843

Ala Ala Glu Gly Val Arg Arg Gly Gly Tyr Val Leu Val Glu Gly Ser

570 575 580

aag gaa acc cca gat gtg atc ctc atg ggc tcc ggc tcc gag gtt cag 1891

Lys Glu Thr Pro Asp Val Ile Leu Met Gly Ser Gly Ser Glu Val Gln

585 590 595

ctt gca gtt aac gct gcg aag gct ctg gaa gct gag ggc gtt gca gct 1939

Leu Ala Val Asn Ala Ala Lys Ala Leu Glu Ala Glu Gly Val Ala Ala

600 605 610

cgc gtt gtt tcc gtt cct tgc atg gat tgg ttc cag gag cag gac gca 1987

Arg Val Val Ser Val Pro Cys Met Asp Trp Phe Gln Glu Gln Asp Ala

615 620 625

gag tac atc gag tcc gtt ctg cct gca gct gtg acc gct cgt gtg tct 2035

Glu Tyr Ile Glu Ser Val Leu Pro Ala Ala Val Thr Ala Arg Val Ser

630 635 640 645

gtt gaa gct ggc atc gca atg cct tgg tac cgc ttc ttg ggc acc cag 2083

Val Glu Ala Gly Ile Ala Met Pro Trp Tyr Arg Phe Leu Gly Thr Gln

650 655 660

ggc cgt gct gtc tcc ctt gag cacttc ggt gct tct gcg gat tac cag 2131

Gly Arg Ala Val Ser Leu Glu His Phe Gly Ala Ser Ala Asp Tyr Gln

665 670 675

acc ctg ttt gag aag ttc ggc atc acc acc gat gca gtc gtg gca gcg 2179

Thr Leu Phe Glu Lys Phe Gly Ile Thr Thr Asp Ala Val Val Ala Ala

680 685 690

gcc aag gac tcc att aac ggt taattgccct gctgttttta gcttcaaccc 2230

Ala Lys Asp Ser Ile Asn Gly

95 700

<210>100

<211>700

<212>PRT

<213>谷氨酸棒状杆菌

<400>100

Leu Thr Thr Leu Thr Leu Ser Pro Glu Leu Gln Ala Leu Thr Val Arg

1 5 10 15

Asn Tyr Pro Ser Asp Trp Ser Asp Val Asp Thr Lys Ala Val Asp Thr

20 25 30

Val Arg Val Leu Ala Ala Asp Ala Val Glu Asn Cys Gly Ser Gly His

35 40 45

Pro Gly Thr Ala Met Ser Leu Ala Pro Leu Ala Tyr Thr Leu Tyr Gln

50 55 60

Arg Val Met Asn Val Asp Pro Gln Asp Thr Asn Trp Ala Gly Arg Asp

65 70 75 80

Arg Phe Val Leu Ser Cys Gly His Ser Ser Leu Thr Gln Tyr Ile Gln

85 90 95

Leu Tyr Leu Gly Gly Phe Gly Leu Glu Met Asp Asp Leu Lys Ala Leu

100 105 110

Arg Thr Trp Asp Ser Leu Thr Pro Gly His Pro Glu Tyr Arg His Thr

115 120 125

Lys Gly Val Glu Ile Thr Thr Gly Pro Leu Gly Gln Gly Leu Ala Ser

130 135 140

Ala Val Gly Met Ala Met Ala Ala Arg Arg Glu Arg Gly Leu Phe Asp

145 150 155 160

Pro Thr Ala Ala Glu Gly Glu Ser Pro Phe Asp His His Ile Tyr Val

165 170 175

Ile Ala Ser Asp Gly Asp Leu Gln Glu Gly Val Thr Ser Glu Ala Ser

180 185 190

Ser Ile Ala Gly Thr Gln Gln Leu Gly Asn Leu Ile Val Phe Trp Asp

195 200 205

Asp Asn Arg Ile Ser Ile Glu Asp Asn Thr Glu Ile Ala Phe Asn Glu

210 215 220

Asp Val Val Ala Arg Tyr Lys Ala Tyr Gly Trp Gln Thr Ile Glu Val

225 230 235 240

Glu Ala Gly Glu Asp Val Ala Ala Ile Glu Ala Ala Val Ala Glu Ala

245 250 255

Lys Lys Asp Thr Lys Arg Pro Thr Phe Ile Arg Val Arg Thr Ile Ile

260 265 270

Gly Phe Pro Ala Pro Thr Met Met Asn Thr Gly Ala Val His Gly Ala

275 280 285

Ala Leu Gly Ala Ala Glu Val Ala Ala Thr Lys Thr Glu Leu Gly Phe

290 295 300

Asp Pro Glu Ala His Phe Ala Ile Asp Asp Glu Val Ile Ala His Thr

305 310 315 320

Arg Ser Leu Ala Glu Arg Ala Ala Gln Lys Lys Ala Ala Trp Gln Val

325 330 335

Lys Phe Asp Glu Trp Ala Ala Ala Asn Pro Glu Asn Lys Ala Leu Phe

340 345 350

Asp Arg Leu Asn Ser Arg Glu Leu Pro Ala Gly Tyr Ala Asp Glu Leu

355 360 365

Pro Thr Trp Asp Ala Asp Glu Lys Gly Val Ala Thr Arg Lys Ala Ser

370 375 380

Glu Ala Ala Leu Gln Ala Leu Gly Lys Thr Leu Pro Glu Leu Trp Gly

385 390 395 400

Gly Ser Ala Asp Leu Ala Gly Ser Asn Asn Thr Val Ile Lys Gly Ser

405 410 415

Pro Ser Phe Gly Pro Glu Ser Ile Ser Thr Glu Thr Trp Ser Ala Glu

420 425 430

Pro Tyr Gly Arg Asn Leu His Phe Gly Ile Arg Glu His Ala Met Gly

435 440 445

Ser Ile Leu Asn Gly Ile Ser Leu His Gly Gly Thr Arg Pro Tyr Gly

450 455 460

Gly Thr Phe Leu Ile Phe Ser Asp Tyr Met Arg Pro Ala Val Arg Leu

465 470 475 480

Ala Ala Leu Met Glu Thr Asp Ala Tyr Tyr Val Trp Thr His Asp Ser

485 490 495

Ile Gly Leu Gly Glu Asp Gly Pro Thr His Gln Pro Val Glu Thr Leu

500 505 510

Ala Ala Leu Arg Ala Ile Pro Gly Leu Ser Val Leu Arg Pro Ala Asp

515 520 525

Ala Asn Glu Thr Ala Gln Ala Trp Ala Ala Ala Leu Glu Tyr Lys Glu

530 535 540

Gly Pro Lys Gly Leu Ala Leu Thr Arg Gln Asn Val Pro Val Leu Glu

545 550 555 560

Gly Thr Lys Glu Lys Ala Ala Glu Gly Val Arg Arg Gly Gly Tyr Val

565 570 575

Leu Val Glu Gly Ser Lys Glu Thr Pro Asp Val Ile Leu Met Gly Ser

580 585 590

Gly Ser Glu Val Gln Leu Ala Val Asn Ala Ala Lys Ala Leu Glu Ala

595 600 605

Glu Gly Val Ala Ala Arg Val Val Ser Val Pro Cys Met Asp Trp Phe

610 615 620

Gln Glu Gln Asp Ala Glu Tyr Ile Glu Ser Val Leu Pro Ala Ala Val

625 630 635 640

Thr Ala Arg Val Ser Val Glu Ala Gly Ile Ala Met Pro Trp Tyr Arg

645 650 655

Phe Leu Gly Thr Gln Gly Arg Ala Val Ser Leu Glu His Phe Gly Ala

660 665 670

Ser Ala Asp Tyr Gln Thr Leu Phe Glu Lys Phe Gly Ile Thr Thr Asp

675 680 685

Ala Val Val Ala Ala Ala Lys Asp Ser Ile Asn Gly

690 695 700

<210>101

<211>1099

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1069)

<223>RXA02740

<400>101

agtaaacact ctgacgcttc tgtcagagtc tgggccttca acgcaaggtg cacactcacc 60

gcagggatat ttaaataata aggactcaca acttaaatat atg agt gat ttg aaa 115

Met Ser Asp Leu Lys

1 5

atg caa cgt tct gga gga gaa ccc ttg gac acg atc aag gcc tat att 163

Met Gln Arg Ser Gly Gly Glu Pro Leu Asp Thr Ile Lys Ala Tyr Ile

10 15 20

gcg cta acg aag ccc agg gtt att gaa ctc ctc ctt gtc gcc aca atc 211

Ala Leu Thr Lys Pro Arg Val Ile Glu Leu Leu Leu Val Ala Thr Ile

25 30 35

ccc aca atg ctt cag gct gaa cgc ggt gag aac aac att gtg ctc atc 259

Pro Thr Met Leu Gln Ala Glu Arg Gly Glu Asn Asn Ile Val Leu Ile

40 45 50

ttg ctg act gtg ttc ggt ggc tgg atg ggt gcg gcc gcc gcc aac acc 307

Leu Leu Thr Val Phe Gly Gly Trp Met Gly Ala Ala Ala Ala Asn Thr

55 60 65

ttc aac atg gtg gca gac tcc gat att gat cag cgc atg gga cgc act 355

Phe Asn Met Val Ala Asp Ser Asp Ile Asp Gln Arg Met Gly Arg Thr

70 75 80 85

agg gct cgc cct ttg gtg cgc cac acc gtg agt aat cgc gac gcc tcc 403

Arg Ala Arg Pro Leu Val Arg His Thr Val Ser Asn Arg Asp Ala Ser

90 95 100

att ttt gcg tgg gtc ctg aca gtg gcc agc ttc ttg tgg ctg tgg ctg 451

Ile Phe Ala Trp Val Leu Thr Val Ala Ser Phe Leu Trp Leu Trp Leu

105 110 115

ctg tgc gat tcg atg ctc gcc ggc atc ttc gtg ttg atc acg att ttc 499

Leu Cys Asp Ser Met Leu Ala Gly Ile Phe Val Leu Ile Thr Ile Phe

120 125 130

ttc tac att ttt gtc tac acc aag tgg ctg aag cgc cgc acg cac atg 547

Phe Tyr Ile Phe Val Tyr Thr Lys Trp Leu Lys Arg Arg Thr His Met

135 140 145

aat atc gtg tgg ggc gga gcc gca ggt tgt atg cca gtg ctc gtc ggc 595

Asn Ile Val Trp Gly Gly Ala Ala Gly Cys Met Pro Val Leu Val Gly

150 155 160 165

tgg gca gtg atc gtt gat cag ttt gag cca ggc gtt cca cag cag tgg 643

Trp Ala Val Ile Val Asp Gln Phe Glu Pro Gly Val Pro Gln Gln Trp

170 175 180

tgg cag gca att gtc ctg ttc atg gtg att ttc ttc tgg acc cca cct 691

Trp Gln Ala Ile Val Leu Phe Met Val Ile Phe Phe Trp Thr Pro Pro

185 190 195

cac acc tgg gct ctg gcc atg aag tac cgc gaa gac tac aag gcg gct 739

His Thr Trp Ala Leu Ala Met Lys Tyr Arg Glu Asp Tyr Lys Ala Ala

200 205 210

ggc gtc cca atg ctt cct gtc gtg cgc acc cca gtc cag gtc acc gca 787

Gly Val Pro Met Leu Pro Val Val Arg Thr Pro Val Gln Val Thr Ala

215 220 225

caa atc gtg tgg tac tcc gtg gca act gtg ctg acc acc ttc ttg ctc 835

Gln Ile Val Trp Tyr Ser Val Ala Thr Val Leu Thr Thr Phe Leu Leu

230 235 240 245

atc cca gca act ggt tgg atc tac gca gcg atc gcc gtc att tcc ggc 883

Ile Pro Ala Thr Gly Trp Ile Tyr Ala Ala Ile Ala Val Ile Ser Gly

250 255 260

gtc acc ttc ttg ttc atg gcc atc aag ctg cac ctc ggc atc aaa aac 931

Val Thr Phe Leu Phe Met Ala Ile Lys Leu His Leu Gly Ile Lys Asn

265 270 275

ggt ggc aag gtc aag cct ctg aag ctg ttt att ttg tcc aac aac tac 979

Gly Gly Lys Val Lys Pro Leu Lys Leu Phe Ile Leu Ser Asn Asn Tyr

280 285 290

ttg gca gtc ctc ttc gtg gca ttg tcc gtc gac gcg gtc ctc ggc ctt 1027

Leu Ala Val Leu Phe Val Ala Leu Ser Val Asp Ala Val Leu Gly Leu

295 300 305

gag acc atc ggc gag atg ctc ggc tgg acc acc acc ttc ttc 1069

Glu Thr Ile Gly Glu Met Leu Gly Trp Thr Thr Thr Phe Phe

310 315 320

taaaagcttg cttttcgacg aaaagatcct 1099

<210>102

<211>323

<212>PRT

<213>谷氨酸棒状杆菌

<400>102

Met Ser Asp Leu Lys Met Gln Arg Ser Gly Gly Glu Pro Leu Asp Thr

1 5 10 15

Ile Lys Ala Tyr Ile Ala Leu Thr Lys Pro Arg Val Ile Glu Leu Leu

20 25 30

Leu Val Ala Thr Ile Pro Thr Met Leu Gln Ala Glu Arg Gly Glu Asn

35 40 45

Asn Ile Val Leu Ile Leu Leu Thr Val Phe Gly Gly Trp Met Gly Ala

50 55 60

Ala Ala Ala Asn Thr Phe Asn Met Val Ala Asp Ser Asp Ile Asp Gln

65 70 75 80

Arg Met Gly Arg Thr Arg Ala Arg Pro Leu Val Arg His Thr Val Ser

85 90 95

Asn Arg Asp Ala Ser Ile Phe Ala Trp Val Leu Thr Val Ala Ser Phe

100 105 110

Leu Trp Leu Trp Leu Leu Cys Asp Ser Met Leu Ala Gly Ile Phe Val

115 120 125

Leu Ile Thr Ile Phe Phe Tyr Ile Phe Val Tyr Thr Lys Trp Leu Lys

130 135 140

Arg Arg Thr His Met Asn Ile Val Trp Gly Gly Ala Ala Gly Cys Met

145 150 155 160

Pro Val Leu Val Gly Trp Ala Val Ile Val Asp Gln Phe Glu Pro Gly

165 170 175

Val Pro Gln Gln Trp Trp Gln Ala Ile Val Leu Phe Met Val Ile Phe

180 185 190

Phe Trp Thr Pro Pro His Thr Trp Ala Leu Ala Met Lys Tyr Arg Glu

195 200 205

Asp Tyr Lys Ala Ala Gly Val Pro Met Leu Pro Val Val Arg Thr Pro

210 215 220

Val Gln Val Thr Ala Gln Ile Val Trp Tyr Ser Val Ala Thr Val Leu

225 230 235 240

Thr Thr Phe Leu Leu Ile Pro Ala Thr Gly Trp Ile Tyr Ala Ala Ile

245 250 255

Ala Val Ile Ser Gly Val Thr Phe Leu Phe Met Ala Ile Lys Leu His

260 265 270

Leu Gly Ile Lys Asn Gly Gly Lys Val Lys Pro Leu Lys Leu Phe Ile

275 280 285

Leu Ser Asn Asn Tyr Leu Ala Val Leu Phe Val Ala Leu Ser Val Asp

290 295 300

Ala Val Leu Gly Leu Glu Thr Ile Gly Glu Met Leu Gly Trp Thr Thr

305 310 315 320

Thr Phe Phe

<210>103

<211>694

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(664)

<223>RXA03030

<400>103

gaagatgaag cagaaaagat cattggtgcg ccagaggttt ctgcattggg caacaaagca 60

cagcttgatt ccgtcacctt gctgcgtaac aaccccatcc gtg ctg cca ctg gat 115

Val Leu Pro Leu Asp

1 5

cct gca gca agc ctg aag atc tac cca ttg gtt act ggc cgt acc aag 163

Pro Ala Ala Ser Leu Lys Ile Tyr Pro Leu Val Thr Gly Arg Thr Lys

10 15 20

atc gac gag gtt caa cta cag ctg gaa gca gcc att cgc gca gaa ctc 211

Ile Asp Glu Val Gln Leu Gln Leu Glu Ala Ala Ile Arg Ala Glu Leu

25 30 35

cca ggg gta acc ttg gtg tct tcc gag tca gaa gca gat ctt gca atc 259

Pro Gly Val Thr Leu Val Ser Ser Glu Ser Glu Ala Asp Leu Ala Ile

40 45 50

gtg tgg gct cgc cct gaa att gca ctg ttt gaa gat gac ctc gaa ggt 307

Val Trp Ala Arg Pro Glu Ile Ala Leu Phe Glu Asp Asp Leu Glu Gly

55 60 65

gtt tcc ctc tct gtt gac cct cgt gcc aat ggt gtc gat gtg gaa cgc 355

Val Ser Leu Ser Val Asp Pro Arg Ala Asn Gly Val Asp Val Glu Arg

70 75 80 85

gtt cag gct gtg gaa gct gca gtc cca acc atc ttg gct gtg aac ttc 403

Val Gln Ala Val Glu Ala Ala Val Pro Thr Ile Leu Ala Val Asn Phe

90 95 100

acc aac cct tgg gtg ctg tct gag atc gag cct ggt gcc gct gcc gtg 451

Thr Asn Pro Trp Val Leu Ser Glu Ile Glu Pro Gly Ala Ala Ala Val

105 110 115

gtg ggc act ttt gag atc aag cca gag ttc ctt ctc aag gct ttg act 499

Val Gly Thr Phe Glu Ile Lys Pro Glu Phe Leu Leu Lys Ala Leu Thr

120 125 130

ggt caa gag gga gga cca aag ggc aag ctg cca ttg act gtt cct gct 547

Gly Gln Glu Gly Gly Pro Lys Gly Lys Leu Pro Leu Thr Val Pro Ala

135 140 145

tcc atg cag gcg att gct gat tcc cct cgc gat atc cca ggc aag ttc 595

Ser Met Gln Ala Ile Ala Asp Ser Pro Arg Asp Ile Pro Gly Lys Phe

150 155 160 165

ctc gat gag tct tac acc tac gtg gac tcc gca ggg atg gcc tac aag 643

Leu Asp Glu Ser Tyr Thr Tyr Val Asp Ser Ala Gly Met Ala Tyr Lys

170 175 180

tac ggt cac gga ctt aat ttc tagattgtag gtagtctcgt gggcacaact 694

Tyr Gly His Gly Leu Asn Phe

185

<210>104

<211>188

<212>PRT

<213>谷氨酸棒状杆菌

<400>104

Val Leu Pro Leu Asp Pro Ala Ala Ser Leu Lys Ile Tyr Pro Leu Val

1 5 10 15

Thr Gly Arg Thr Lys Ile Asp Glu Val Gln Leu Gln Leu Glu Ala Ala

20 25 30

Ile Arg Ala Glu Leu Pro Gly Val Thr Leu Val Ser Ser Glu Ser Glu

35 40 45

Ala Asp Leu Ala Ile Val Trp Ala Arg Pro Glu Ile Ala Leu Phe Glu

50 55 60

Asp Asp Leu Glu Gly Val Ser Leu Ser Val Asp Pro Arg Ala Asn Gly

65 70 75 80

Val Asp Val Glu Arg Val Gln Ala Val Glu Ala Ala Val Pro Thr Ile

85 90 95

Leu Ala Val Asn Phe Thr Asn Pro Trp Val Leu Ser Glu Ile Glu Pro

100 105 110

Gly Ala Ala Ala Val Val Gly Thr Phe Glu Ile Lys Pro Glu Phe Leu

115 120 125

Leu Lys Ala Leu Thr Gly Gln Glu Gly Gly Pro Lys Gly Lys Leu Pro

130 135 140

Leu Thr Val Pro Ala Ser Met Gln Ala Ile Ala Asp Ser Pro Arg Asp

145 150 155 160

Ile Pro Gly Lys Phe Leu Asp Glu Ser Tyr Thr Tyr Val Asp Ser Ala

165 170 175

Gly Met Ala Tyr Lys Tyr Gly His Gly Leu Asn Phe

180 185

<210>105

<211>1537

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1507)

<223>RXA03083

<400>105

gtcacccagg ctaattcagc agtaatcatt tagacttgga accgcttacc agtggtttca 60

acaatgcatt cacccagctc acacgtgtgg aggtgcctta atg gca aag agg atc 115

Met Ala Lys Arg Ile

1 5

gta att atc ggc ggt gga cct gca ggc tat gaa gcc gca ctc gca ggc 163

Val Ile Ile Gly Gly Gly Pro Ala Gly Tyr Glu Ala Ala Leu Ala Gly

10 15 20

gct aaa tac ggt gca gaa gtt acc gtt att gaa gat gtc gga gtt ggc 211

Ala Lys Tyr Gly Ala Glu Val Thr Val Ile Glu Asp Val Gly Val Gly

25 30 35

gga tcc gca gtc acc atg gac tgt gta cct tca aag tcc ttc atc gct 259

Gly Ser Ala Val Thr Met Asp Cys Val Pro Ser Lys Ser Phe Ile Ala

40 45 50

ggt acc ggt atc aaa acc gac ctc cga cgt gct gat gac atg gga ctt 307

Gly Thr Gly Ile Lys Thr Asp Leu Arg Arg Ala Asp Asp Met Gly Leu

55 60 65

aac cgt ggg ctt gga aaa gca cac cta gaa atc gat gca ctg aac atc 355

Asn Arg Gly Leu Gly Lys Ala His Leu Glu Ile Asp Ala Leu Asn Ile

70 75 80 85

cgt gtg aag gac ctt gcg aaa gca cag tcc gaa gat atc ttg ggc cag 403

Arg Val Lys Asp Leu Ala Lys Ala Gln Ser Glu Asp Ile Leu Gly Gln

90 95 100

ctg cag cgc tca gat gtc cgc atg att aac ggt gtg ggc cgc ttt gat 451

Leu Gln Arg Ser Asp Val Arg Met Ile Asn Gly Val Gly Arg Phe Asp

105 110 115

gat tac aac acc aag caa acc acc cac tac att aaa gtc acc cac agc 499

Asp Tyr Asn Thr Lys Gln Thr Thr His Tyr Ile Lys Val Thr His Ser

120 125 130

gat ggc tcc gaa gaa acc gtt gag tgc gat ctg gtg ctg gtt gca act 547

Asp Gly Ser Glu Glu Thr Val Glu Cys Asp Leu Val Leu Val Ala Thr

135 140 145

ggt gca acc ccc cgc att ctt aaa ggt gca gag cca gac ggc gag cgc 595

Gly Ala Thr Pro Arg Ile Leu Lys Gly Ala Glu Pro Asp Gly Glu Arg

150 155 160 165

atc ttg acc tgg cgt cag gtc tac gac att gaa gaa ctc ccc acc cac 643

Ile Leu Thr Trp Arg Gln Val Tyr Asp Ile Glu Glu Leu Pro Thr His

170 175 180

ctt atc gtg gtt ggt tcc ggt gtg acc ggt gcg gaa ttt gtc tct gcg 691

Leu Ile Val Val Gly Ser Gly Val Thr Gly Ala Glu Phe Val Ser Ala

185 190 195

ttt gct gaa ctc ggc gtc aaa gtc acc atg gtg gca tcc cgt gac cgc 739

Phe Ala Glu Leu Gly Val Lys Val Thr Met Val Ala Ser Arg Asp Arg

200 205 210

att ttg cct cac gat gac gca gat gcc gca gac gtg ctg gaa acc gtt 787

Ile Leu Pro His Asp Asp Ala Asp Ala Ala Asp Val Leu Glu Thr Val

215 220 225

ctg gct gag cgc gga gta tcc ctg gaa aag cat gcc cgc gtg gag tct 835

Leu Ala Glu Arg Gly Val Ser Leu Glu Lys His Ala Arg Val Glu Ser

230 235 240 245

gtc acc cgc acc gaa gac ggt ggc gtg tgt gtt cgc act gct gac gga 883

Val Thr Arg Thr Glu Asp Gly Gly Val Cys Val Arg Thr Ala Asp Gly

250 255 260

cga gaa atc tac ggt tct cac gcg ttg atg act gtt ggt tcc att cca 931

Arg Glu Ile Tyr Gly Ser His Ala Leu Met Thr Val Gly Ser Ile Pro

265 270 275

aac acg gca gat ctt ggc ctg gag aac atc ggt gtt gag ctg gca cca 979

Asn Thr Ala Asp Leu Gly Leu Glu Asn Ile Gly Val Glu Leu Ala Pro

280 285 290

tcc ggc cat atc aag gtt gac cgc gtc tcc cgc acc aac atc ccc ggt 1027

Ser Gly His Ile Lys Val Asp Arg Val Ser Arg Thr Asn Ile Pro Gly

295 300 305

gtg tac gca gca ggt gac tgt act gac cta ttc cca ctg gcg tcc gtt 1075

Val Tyr Ala Ala Gly Asp Cys Thr Asp Leu Phe Pro Leu Ala Ser Val

310 315 320 325

gca gcg atg cag ggc cgt atc gcc atg tat cac gca ctc ggt gaa ggc 1123

Ala Ala Met Gln Gly Arg Ile Ala Met Tyr His Ala Leu Gly Glu Gly

330 335 340

gtg agc ccc atc cgt ttg aag act gtt gcc acc gca gtg ttt acc cgc 1171

Val Ser Pro Ile Arg Leu Lys Thr Val Ala Thr Ala Val Phe Thr Arg

345 350 355

cca gag atc gca gca gta ggt atc acc cat gca caa gtt gat tcc ggc 1219

Pro Glu Ile Ala Ala Val Gly Ile Thr His Ala Gln Val Asp Ser Gly

360 365 370

gaa gtg tct gct cgc gtg att gtg ctt cct ttg gct act aac cca cgc 1267

Glu Val Ser Ala Arg Val Ile Val Leu Pro Leu Ala Thr Asn Pro Arg

375 380 385

gcc aag atg cgt tcc ctg cgc cac ggt ttt gtg aag ctg ttc tgc cgc 1315

Ala Lys Met Arg Ser Leu Arg His Gly Phe Val Lys Leu Phe Cys Arg

390 395 400 405

cgt aac tct ggc ctg atc atc ggt ggt gtc gtg gtg gca ccg acc gcg 1363

Arg Asn Ser Gly Leu Ile Ile Gly Gly Val Val Val Ala Pro Thr Ala

410 415 420

tct gag ctg atc cta ccg atc gct gtg gca gtg acc aac cgt ctg aca 1411

Ser Glu Leu Ile Leu Pro Ile Ala Val Ala Val Thr Asn Arg Leu Thr

425 430 435

gtt gct gat ctg gct gat acc ttc gcg gtg tac cca tca ttg tca ggt 1459

Val Ala Asp Leu Ala Asp Thr Phe Ala Val Tyr Pro Ser Leu Ser Gly

440 445 450

tcg att act gaa gca gca cgt cag ctg gtt caa cat gat gat cta ggc 1507

Ser Ile Thr Glu Ala Ala Arg Gln Leu Val Gln His Asp Asp Leu Gly

455 460 465

taatttttct gagtcttaga ttttgagaaa 1537

<210>106

<211>469

<212>PRT

<213>谷氨酸棒状杆菌

<400>106

Met Ala Lys Arg Ile Val Ile Ile Gly Gly Gly Pro Ala Gly Tyr Glu

1 5 10 15

Ala Ala Leu Ala Gly Ala Lys Tyr Gly Ala Glu Val Thr Val Ile Glu

20 25 30

Asp Val Gly Val Gly Gly Ser Ala Val Thr Met Asp Cys Val Pro Ser

35 40 45

Lys Ser Phe Ile Ala Gly Thr Gly Ile Lys Thr Asp Leu Arg Arg Ala

50 55 60

Asp Asp Met Gly Leu Asn Arg Gly Leu Gly Lys Ala His Leu Glu Ile

65 70 75 80

Asp Ala Leu Asn Ile Arg Val Lys Asp Leu Ala Lys Ala Gln Ser Glu

85 90 95

Asp Ile Leu Gly Gln Leu Gln Arg Ser Asp Val Arg Met Ile Asn Gly

100 105 110

Val Gly Arg Phe Asp Asp Tyr Asn Thr Lys Gln Thr Thr His Tyr Ile

115 120 125

Lys Val Thr His Ser Asp Gly Ser Glu Glu Thr Val Glu Cys Asp Leu

130 135 140

Val Leu Val Ala Thr Gly Ala Thr Pro Arg Ile Leu Lys Gly Ala Glu

145 150 155 160

Pro Asp Gly Glu Arg Ile Leu Thr Trp Arg Gln Val Tyr Asp Ile Glu

165 170 175

Glu Leu Pro Thr His Leu Ile Val Val Gly Ser Gly Val Thr Gly Ala

180 185 190

Glu Phe Val Ser Ala Phe Ala Glu Leu Gly Val Lys Val Thr Met Val

195 200 205

Ala Ser Arg Asp Arg Ile Leu Pro His Asp Asp Ala Asp Ala Ala Asp

210 215 220

Val Leu Glu Thr Val Leu Ala Glu Arg Gly Val Ser Leu Glu Lys His

225 230 235 240

Ala Arg Val Glu Ser Val Thr Arg Thr Glu Asp Gly Gly Val Cys Val

245 250 255

Arg Thr Ala Asp Gly Arg Glu Ile Tyr Gly Ser His Ala Leu Met Thr

260 265 270

Val Gly Ser Ile Pro Asn Thr Ala Asp Leu Gly Leu Glu Asn Ile Gly

275 280 285

Val Glu Leu Ala Pro Ser Gly His Ile Lys Val Asp Arg Val Ser Arg

290 295 300

Thr Asn Ile Pro Gly Val Tyr Ala Ala Gly Asp Cys Thr Asp Leu Phe

305 310 315 320

Pro Leu Ala Ser Val Ala Ala Met Gln Gly Arg Ile Ala Met Tyr His

325 330 335

Ala Leu Gly Glu Gly Val Ser Pro Ile Arg Leu Lys Thr Val Ala Thr

340 345 350

Ala Val Phe Thr Arg Pro Glu Ile Ala Ala Val Gly Ile Thr His Ala

355 360 365

Gln Val Asp Ser Gly Glu Val Ser Ala Arg Val Ile Val Leu Pro Leu

370 375 380

Ala Thr Asn Pro Arg Ala Lys Met Arg Ser Leu Arg His Gly Phe Val

385 390 395 400

Lys Leu Phe Cys Arg Arg Asn Ser Gly Leu Ile Ile Gly Gly Val Val

405 410 415

Val Ala Pro Thr Ala Ser Glu Leu Ile Leu Pro Ile Ala Val Ala Val

420 425 430

Thr Asn Arg Leu Thr Val Ala Asp Leu Ala Asp Thr Phe Ala Val Tyr

435 440 445

Pro Ser Leu Ser Gly Ser Ile Thr Glu Ala Ala Arg Gln Leu Val Gln

450 455 460

His Asp Asp Leu Gly

465

<210>107

<211>919

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(889)

<223>RXA03150

<400>107

acacttcttc gcgggataca gtcatgacag tgcaatctca ctgtccatag aaatgtaact 60

catatggaat cgcagtatca taaattgcaa tctccccggg ttg cag tcg caa atc 115

Leu Gln Ser Gln Ile

1 5

tgg ctc tcc atc ccg ggt gag cac cat ggt ctc gga ttt cag tgc aca 163

Trp Leu Ser Ile Pro Gly Glu His His Gly Leu Gly Phe Gln Cys Thr

10 15 20

tac ttt aac aga gtg cgt ttc aat gcc tgt agt gtt ccg gca att ttg 211

Tyr Phe Asn Arg Val Arg Phe Asn Ala Cys Ser Val Pro Ala Ile Leu

25 30 35

aat gtc gtt acg tta ccc aag gct gaa ttc ctg agc tca cct tgt aca 259

Asn Val Val Thr Leu Pro Lys Ala Glu Phe Leu Ser Ser Pro Cys Thr

40 45 50

aga tca gtg gaa gcc cag ttc acc tct ccc ctg ctc aac aat ggg caa 307

Arg Ser Val Glu Ala Gln Phe Thr Ser Pro Leu Leu Asn Asn Gly Gln

55 60 65

acc tgt ttc ctt ggt acc cga atc ctt gct cca aaa tca cgt tac gcg 355

Thr Cys Phe Leu Gly Thr Arg Ile Leu Ala Pro Lys Ser Arg Tyr Ala

70 75 80 85

gaa gta gtc gat gca ttc acc gct ttc gct ggc agc ctg cag gtt gga 403

Glu Val Val Asp Ala Phe Thr Ala Phe Ala Gly Ser Leu Gln Val Gly

90 95 100

gtc acg tcc tcc cct gac act cag atc gga ccg atg gcg act gcc cgg 451

Val Thr Ser Ser Pro Asp Thr Gln Ile Gly Pro Met Ala Thr Ala Arg

105 110 115

cag cgt gag cgc gtg gaa tcc tac att tcc caa ggc aaa aat gct gga 499

Gln Arg Glu Arg Val Glu Ser Tyr Ile Ser Gln Gly Lys Asn Ala Gly

120 125 130

gcc cgc atc act gtc ggt ggc agc cgt cca cga gat ctt gac gcc gga 547

Ala Arg Ile Thr Val Gly Gly Ser Arg Pro Arg Asp Leu Asp Ala Gly

135 140 145

ttc ttc gtt gag cca aca gtg ttc gcc gat gta gac aat cgc gca gcc 595

Phe Phe Val Glu Pro Thr Val Phe Ala Asp Val Asp Asn Arg Ala Ala

150 155 160 165

att gcc caa gat gaa atc ttc gga ccg gtg ccc tct gtt gtt tcc tac 643

Ile Ala Gln Asp Glu Ile Phe Gly Pro Val Pro Ser Val Val Ser Tyr

170 175 180

caa gac gat gaa cac gcc atc caa cta gcc aac gat tcc gaa ttc ggt 691

Gln Asp Asp Glu His Ala Ile Gln Leu Ala Asn Asp Ser Glu Phe Gly

185 190 195

ctc ggc gga act gtc tgg acg agc gat ccc gag cgc ggc gct gca ttg 739

Leu Gly Gly Thr Val Trp Thr Ser Asp Pro Glu Arg Gly Ala Ala Leu

200 205 210

gcc cgc cga gtt cac aca gga acc att ggc atc aac cgc tat atc cct 787

Ala Arg Arg Val His Thr Gly Thr Ile Gly Ile Asn Arg Tyr Ile Pro

215 220 225

gat ccc gcc gca cca ttt gga ggt gtg aaa aac agt ggc ctt ggc aga 835

Asp Pro Ala Ala Pro Phe Gly Gly Val Lys Asn Ser Gly Leu Gly Arg

230 235 240 245

gaa ctc ggc ccc gaa ggt ctt gct tcc tac caa gaa acc caa acc att 883

Glu Leu Gly Pro Glu Gly Leu Ala Ser Tyr Gln Glu Thr Gln Thr Ile

250 255 260

tat ctc taatccaaac tgcacctata tataaggagt 919

Tyr Leu

<210>108

<211>263

<212>PRT

<213>谷氨酸棒状杆菌

<400>108

Leu Gln Ser Gln Ile Trp Leu Ser Ile Pro Gly Glu His His Gly Leu

1 5 10 15

Gly Phe Gln Cys Thr Tyr Phe Asn Arg Val Arg Phe Asn Ala Cys Ser

20 25 30

Val Pro Ala Ile Leu Asn Val Val Thr Leu Pro Lys Ala Glu Phe Leu

35 40 45

Ser Ser Pro Cys Thr Arg Ser Val Glu Ala Gln Phe Thr Ser Pro Leu

50 55 60

Leu Asn Asn Gly Gln Thr Cys Phe Leu Gly Thr Arg Ile Leu Ala Pro

65 70 75 80

Lys Ser Arg Tyr Ala Glu Val Val Asp Ala Phe Thr Ala Phe Ala Gly

85 90 95

Ser Leu Gln Val Gly Val Thr Ser Ser Pro Asp Thr Gln Ile Gly Pro

100 105 110

Met Ala Thr Ala Arg Gln Arg Glu Arg Val Glu Ser Tyr Ile Ser Gln

115 120 125

Gly Lys Asn Ala Gly Ala Arg Ile Thr Val Gly Gly Ser Arg Pro Arg

130 135 140

Asp Leu Asp Ala Gly Phe Phe Val Glu Pro Thr Val Phe Ala Asp Val

145 150 155 160

Asp Asn Arg Ala Ala Ile Ala Gln Asp Glu Ile Phe Gly Pro Val Pro

165 170 175

Ser Val Val Ser Tyr Gln Asp Asp Glu His Ala Ile Gln Leu Ala Asn

180 185 190

Asp Ser Glu Phe Gly Leu Gly Gly Thr Val Trp Thr Ser Asp Pro Glu

195 200 205

Arg Gly Ala Ala Leu Ala Arg Arg Val His Thr Gly Thr Ile Gly Ile

210 215 220

Asn Arg Tyr Ile Pro Asp Pro Ala Ala Pro Phe Gly Gly Val Lys Asn

225 230 235 240

Ser Gly Leu Gly Arg Glu Leu Gly Pro Glu Gly Leu Ala Ser Tyr Gln

245 250 255

Glu Thr Gln Thr Ile Tyr Leu

260

<210>109

<211>1141

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(1111)

<223>RXA03216

<400>109

gctgtatcac agtcagctgc agcaggtggc gaaaccgttg cggtcccagc ggctgctctg 60

atccctgcaa acaactagaa actattcaga aagcatcacc atg aaa ctc ggt ctc 115

Met Lys Leu Gly Leu

1 5

tac aac gcg atc ttc cac gac cgc acc ctg cca gaa gcg ctc gca gcc 163

Tyr Asn Ala Ile Phe His Asp Arg Thr Leu Pro Glu Ala Leu Ala Ala

10 15 20

atc aaa gct gca ggt ctc acc gga att gaa ctc aac acc ggc gga ttt 211

Ile Lys Ala Ala Gly Leu Thr Gly Ile Glu Leu Asn Thr Gly Gly Phe

25 30 35

ttg cct gca acc cac atc ccg acc atc gat gac atc ctg gtc agc gat 259

Leu Pro Ala Thr His Ile Pro Thr Ile Asp Asp Ile Leu Val Ser Asp

40 45 50

gat gcc cgc gat gaa ttc ctc ggg att ttc gaa ggc acc ggc gtc gac 307

Asp Ala Arg Asp Glu Phe Leu Gly Ile Phe Glu Gly Thr Gly Val Asp

55 60 65

atc tac ggc ctt aac tgc aac ggc aac ccg ctt cac ccc aac aag gcg 355

Ile Tyr Gly Leu Asn Cys Asn Gly Asn Pro Leu His Pro Asn Lys Ala

70 75 80 85

atc ggg gac aag cat gcc gaa gac att cga cgt tcc atc cgc ctc gca 403

Ile Gly Asp Lys His Ala Glu Asp Ile Arg Arg Ser Ile Arg Leu Ala

90 95 100

gag cgc ctc ggc caa aac cgt gtg gtc acc atg tct ggt ctt cct ggt 451

Glu Arg Leu Gly Gln Asn Arg Val Val Thr Met Ser Gly Leu Pro Gly

105 110 115

ggc gaa cca ggc gcg aag tac acc aac tgg gtt gtc aac gcg tgg aac 499

Gly Glu Pro Gly Ala Lys Tyr Thr Asn Trp Val Val Asn Ala Trp Asn

120 125 130

tcc gca gcc ttg gat gtc ctt gat tac caa tgg gat atc gca gct gaa 547

Ser Ala Ala Leu Asp Val Leu Asp Tyr Gln Trp Asp Ile Ala Ala Glu

135 140 145

ttc tgg cgc gag acc gac cgc ttc gcc gca gat cac ggc gtg aaa gtg 595

Phe Trp Arg Glu Thr Asp Arg Phe Ala Ala Asp His Gly Val Lys Val

150 155 160 165

gct ctt gag ctg cac cca cag aac atc gtg ttc aac tcc gct gac gtg 643

Ala Leu Glu Leu His Pro Gln Asn Ile Val Phe Asn Ser Ala Asp Val

170 175 180

cat aag ctc atc gat ctc acc ggc gcc acc cac gtg ggc gtc gaa ctg 691

His Lys Leu Ile Asp Leu Thr Gly Ala Thr His Val Gly Val Glu Leu

185 190 195

gat gca tca cac ctg ttc tgg cag cag atg gac cca atc gct gtg att 739

Asp Ala Ser His Leu Phe Trp Gln Gln Met Asp Pro Ile Ala Val Ile

200 205 210

gat cac ctc ggc gag ctc atc ttc cac gcc gcc gcc aaa gac gtg cga 787

Asp His Leu Gly Glu Leu Ile Phe His Ala Ala Ala Lys Asp Val Arg

215 220 225

gtt aat aag gaa tgg gct cag ctc aac ggt gtg ctg gac aac agc ttc 835

Val Asn Lys Glu Trp Ala Gln Leu Asn Gly Val Leu Asp Asn Ser Phe

230 235 240 245

cga cgc ctt gac cca tcc gaa aac cgc acc aac ttg ggc ggc gac gag 883

Arg Arg Leu Asp Pro Ser Glu Asn Arg Thr Asn Leu Gly Gly Asp Glu

250 255 260

tgg gcg aat gaa tgg cca aag aac tct gct tgg gat ttc gtt gct ctg 931

Trp Ala Asn Glu Trp Pro Lys Asn Ser Ala Trp Asp Phe Val Ala Leu

265 270 275

ggc cgc ggt cat gac gtt gct tac tgg acc gaa ttc ctc cgc gca ctt 979

Gly Arg Gly His Asp Val Ala Tyr Trp Thr Glu Phe Leu Arg Ala Leu

280 285 290

cac cgc gtc gat cca aac atg ctg gtc aac atc gaa cac gag gat gtt 1027

His Arg Val Asp Pro Asn Met Leu Val Asn Ile Glu His Glu Asp Val

295 300 305

tca ctc ggt cgc gaa gaa ggc gtc aac gaa gcc gct aag gtg ctg atc 1075

Ser Leu Gly Arg Glu Glu Gly Val Asn Glu Ala Ala Lys Val Leu Ile

310 315 320 325

gag gcc aac aag gca ctc gaa gag tcc ctg gtt tct taaaaaaact 1121

Glu Ala Asn Lys Ala Leu Glu Glu Ser Leu Val Ser

330 335

acgcctgccc cgcaacgctt 1141

<210>110

<211>337

<212>PRT

<213>谷氨酸棒状杆菌

<400>110

Met Lys Leu Gly Leu Tyr Asn Ala Ile Phe His Asp Arg Thr Leu Pro

1 5 10 15

Glu Ala Leu Ala Ala Ile Lys Ala Ala Gly Leu Thr Gly Ile Glu Leu

20 25 30

Asn Thr Gly Gly Phe Leu Pro Ala Thr His Ile Pro Thr Ile Asp Asp

35 40 45

Ile Leu Val Ser Asp Asp Ala Arg Asp Glu Phe Leu Gly Ile Phe Glu

50 55 60

Gly Thr Gly Val Asp Ile Tyr Gly Leu Asn Cys Asn Gly Asn Pro Leu

65 70 75 80

His Pro Asn Lys Ala Ile Gly Asp Lys His Ala Glu Asp Ile Arg Arg

85 90 95

Ser Ile Arg Leu Ala Glu Arg Leu Gly Gln Asn Arg Val Val Thr Met

100 105 110

Ser Gly Leu Pro Gly Gly Glu Pro Gly Ala Lys Tyr Thr Asn Trp Val

115 120 125

Val Asn Ala Trp Asn Ser Ala Ala Leu Asp Val Leu Asp Tyr Gln Trp

130 135 140

Asp Ile Ala Ala Glu Phe Trp Arg Glu Thr Asp Arg Phe Ala Ala Asp

145 150 155 160

His Gly Val Lys Val Ala Leu Glu Leu His Pro Gln Asn Ile Val Phe

165 170 175

Asn Ser Ala Asp Val His Lys Leu Ile Asp Leu Thr Gly Ala Thr His

180 185 190

Val Gly Val Glu Leu Asp Ala Ser His Leu Phe Trp Gln Gln Met Asp

195 200 205

Pro Ile Ala Val Ile Asp His Leu Gly Glu Leu Ile Phe His Ala Ala

210 215 220

Ala Lys Asp Val Arg Val Asn Lys Glu Trp Ala Gln Leu Asn Gly Val

225 230 235 240

Leu Asp Asn Ser Phe Arg Arg Leu Asp Pro Ser Glu Asn Arg Thr Asn

245 250 255

Leu Gly Gly Asp Glu Trp Ala Asn Glu Trp Pro Lys Asn Ser Ala Trp

260 265 270

Asp Phe Val Ala Leu Gly Arg Gly His Asp Val Ala Tyr Trp Thr Glu

275 280 285

Phe Leu Arg Ala Leu His Arg Val Asp Pro Asn Met Leu Val Asn Ile

290 295 300

Glu His Glu Asp Val Ser Leu Gly Arg Glu Glu Gly Val Asn Glu Ala

305 310 315 320

Ala Lys Val Leu Ile Glu Ala Asn Lys Ala Leu Glu Glu Ser Leu Val

325 330 335

Ser

<210>111

<211>1156

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<22l>CDS

<222>(101)..(1126)

<223>RXA03388

<400>111

aagaaacgtt ggcaatgatg gctgcgtttt ctcgcgtgcg agaagcctca tccatattgg 60

tgcgcgcttc ccgaagcttc gtgcgcagtt cctgcttagt ttg gct cat aaa tct 115

Leu Ala His Lys Ser

1 5

aag gat aac cgt tat ttt cgg agg ggt acg acg att ggg gtt gcg ggg 163

Lys Asp Asn Arg Tyr Phe Arg Arg Gly Thr Thr Ile Gly Val Ala Gly

10 15 20

gca ggt act ctt ggt tcc atg agt ttg cct atc gat gag cac gtg aac 211

Ala Gly Thr Leu Gly Ser Met Ser Leu Pro Ile Asp Glu His Val Asn

25 30 35

gcg gtt aaa acc gtc gta gtg cct gct gca gga ctg gga acc cga ttc 259

Ala Val Lys Thr Val Val Val Pro Ala Ala Gly Leu Gly Thr Arg Phe

40 45 50

ctt ccg gcc acc aaa acc gta ccc aag gag ttg ctg ccg gtt gtc gat 307

Leu Pro Ala Thr Lys Thr Val Pro Lys Glu Leu Leu Pro Val Val Asp

55 60 65

acc cca ggt att gag ctg att gct gct gag gct gcc gaa ctt ggt gcg 355

Thr Pro Gly Ile Glu Leu Ile Ala Ala Glu Ala Ala Glu Leu Gly Ala

70 75 80 85

acc agg ctg gcg atc atc act gcg cca aac aaa gct ggg gta ctt gca 403

Thr Arg Leu Ala Ile Ile Thr Ala Pro Asn Lys Ala Gly Val Leu Ala

90 95 100

cac ttt gag cgt tct tct gaa ttg gaa gaa acg ctg atg gag cgt ggc 451

His Phe Glu Arg Ser Ser Glu Leu Glu Glu Thr Leu Met Glu Arg Gly

105 110 115

aag act gac cag gtg gag ata atc cgc cgc gcc gcc gat tta atc aag 499

Lys Thr Asp Gln Val Glu Ile Ile Arg Arg Ala Ala Asp Leu Ile Lys

120 125 130

gca gtt cca gta acc cag gac aag ccg ctg ggg cta ggt cat gct gtt 547

Ala Val Pro Val Thr Gln Asp Lys Pro Leu Gly Leu Gly His Ala Val

135 140 145

ggt ttg gct gag tct gtg ttg gat gat gat gaa gat gtc gta gcg gtg 595

Gly Leu Ala Glu Ser Val Leu Asp Asp Asp Glu Asp Val Val Ala Val

150 155 160 165

atg ttg ccg gac gat ctc gtg ttg cca acc ggt gtt atg gag cgc atg 643

Met Leu Pro Asp Asp Leu Val Leu Pro Thr Gly Val Met Glu Arg Met

170 175 180

gct cag gtc cga gca gag ttc ggt gga tct gtc ctt tgt gca gtt gag 691

Ala Gln Val Arg Ala Glu Phe Gly Gly Ser Val Leu Cys Ala Val Glu

185 190 195

gtt tct gaa gct gac gtt tct aag tat ggc atc ttt gaa atc gaa gct 739

Val Ser Glu Ala Asp Val Ser Lys Tyr Gly Ile Phe Glu Ile Glu Ala

200 205 210

gac acc aaa gat tcc gat gtc aaa aag gtc aag ggc atg gtg gaa aaa 787

Asp Thr Lys Asp Ser Asp Val Lys Lys Val Lys Gly Met Val Glu Lys

215 220 225

cca gcc atc gaa gac gcg cca tca cgc ctc gcc gcg act ggc cgc tac 835

Pro Ala Ile Glu Asp Ala Pro Ser Arg Leu Ala Ala Thr Gly Arg Tyr

230 235 240 245

ctg ctt gac cga aaa atc ttt gac gcc ctg cgc cgc atc aca cca ggc 883

Leu Leu Asp Arg Lys Ile Phe Asp Ala Leu Arg Arg Ile Thr Pro Gly

250 255 260

gct ggc ggc gag ttg cag ctc acc gac gcg att gat ttg ctt atc gac 931

Ala Gly Gly Glu Leu Gln Leu Thr Asp Ala Ile Asp Leu Leu Ile Asp

265 270 275

gaa ggc cat ccg gtc cat atc gtc att cac cag ggc aaa cgc cac gat 979

Glu Gly His Pro Val His Ile Val Ile His Gln Gly Lys Arg His Asp

280 285 290

ctt ggc aac cct ggc ggg tac att ccg gcg tgt gtt gat ttc ggt ctg 1027

Leu Gly Asn Pro Gly Gly Tyr Ile Pro Ala Cys Val Asp Phe Gly Leu

295 300 305

tcc cac cct gtt tat ggt gcg cag cta aaa gat gca atc aaa cag att 1075

Ser His Pro Val Tyr Gly Ala Gln Leu Lys Asp Ala Ile Lys Gln Ile

310 315 320 325

ttg gct gaa cat gaa gct gcg gaa cgt att gca gac gat tct caa gta 1123

Leu Ala Glu His Glu Ala Ala Glu Arg Ile Ala Asp Asp Ser Gln Val

330 335 340

aaa tagctgcctg gttccgatta ggaggtccgt 1156

Lys

<210>112

<211>342

<212>PRT

<213>谷氨酸棒状杆菌

<400>112

Leu Ala His Lys Ser Lys Asp Asn Arg Tyr Phe Arg Arg Gly Thr Thr

1 5 10 15

Ile Gly Val Ala Gly Ala Gly Thr Leu Gly Ser Met Ser Leu Pro Ile

20 25 30

Asp Glu His Val Asn Ala Val Lys Thr Val Val Val Pro Ala Ala Gly

35 40 45

Leu Gly Thr Arg Phe Leu Pro Ala Thr Lys Thr Val Pro Lys Glu Leu

50 55 60

Leu Pro Val Val Asp Thr Pro Gly Ile Glu Leu Ile Ala Ala Glu Ala

65 70 75 80

Ala Glu Leu Gly Ala Thr Arg Leu Ala Ile Ile Thr Ala Pro Asn Lys

85 90 95

Ala Gly Val Leu Ala His Phe Glu Arg Ser Ser Glu Leu Glu Glu Thr

100 105 110

Leu Met Glu Arg Gly Lys Thr Asp Gln Val Glu Ile Ile Arg Arg Ala

115 120 125

Ala Asp Leu Ile Lys Ala Val Pro Val Thr Gln Asp Lys Pro Leu Gly

130 135 140

Leu Gly His Ala Val Gly Leu Ala Glu Ser Val Leu Asp Asp Asp Glu

145 150 155 160

Asp Val Val Ala Val Met Leu Pro Asp Asp Leu Val Leu Pro Thr Gly

165 170 175

Val Met Glu Arg Met Ala Gln Val Arg Ala Glu Phe Gly Gly Ser Val

180 185 190

Leu Cys Ala Val Glu Val Ser Glu Ala Asp Val Ser Lys Tyr Gly Ile

195 200 205

Phe Glu Ile Glu Ala Asp Thr Lys Asp Ser Asp Val Lys Lys Val Lys

210 215 220

Gly Met Val Glu Lys Pro Ala Ile Glu Asp Ala Pro Ser Arg Leu Ala

225 230 235 240

Ala Thr Gly Arg Tyr Leu Leu Asp Arg Lys Ile Phe Asp Ala Leu Arg

245 250 255

Arg Ile Thr Pro Gly Ala Gly Gly Glu Leu Gln Leu Thr Asp Ala Ile

260 265 270

Asp Leu Leu Ile Asp Glu Gly His Pro Val His Ile Val Ile His Gln

275 280 285

Gly Lys Arg His Asp Leu Gly Asn Pro Gly Gly Tyr Ile Pro Ala Cys

290 295 300

Val Asp Phe Gly Leu Ser His Pro Val Tyr Gly Ala Gln Leu Lys Asp

305 310 315 320

Ala Ile Lys Gln Ile Leu Ala Glu His Glu Ala Ala Glu Arg Ile Ala

325 330 335

Asp Asp Ser Gln Val Lys

340

<210>113

<211>727

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(697)

<223>RXA04088

<400>113

ctctgcacac gttttattga ttgtgcagca ataaatcgag gttcaaaaca taggaccgca 60

ttagcgatta gcgcgcgctg agctttagtt tacagctaac atg agg tgc ata aac 115

Met Arg Cys Ile Asn

1 5

aaa acg gta gat cag caa atc gca ctg ttt ggg caa tcg gga agc gga 163

Lys Thr Val Asp Gln Gln Ile Ala Leu Phe Gly Gln Ser Gly Ser Gly

10 15 20

aaa act gca tta ttg tgc tct ttt tat ggt acc gca cgg gaa tcc tcg 211

Lys Thr Ala Leu Leu Cys Ser Phe Tyr Gly Thr Ala Arg Glu Ser Ser

25 30 35

caa gag gac gtg aaa tta ttc gag atc tct gcc gaa gat gat cga cac 259

Gln Glu Asp Val Lys Leu Phe Glu Ile Ser Ala Glu Asp Asp Arg His

40 45 50

aca gaa ttg atg aga ctg tat ctg gga atg aga gat gat tcg ctt ttc 307

Thr Glu Leu Met Arg Leu Tyr Leu Gly Met Arg Asp Asp Ser Leu Phe

55 60 65

cca cct gcg aat cga ttt gag tca aaa aat aca gtc ttt tct ctg aaa 355

Pro Pro Ala Asn Arg Phe Glu Ser Lys Asn Thr Val Phe Ser Leu Lys

70 75 80 85

caa aaa ggt gtt ccg att aaa gaa gca cgg aag gca gat cag gtc cgg 403

Gln Lys Gly Val Pro Ile Lys Glu Ala Arg Lys Ala Asp Gln Val Arg

90 95 100

gtg acc tgg aat gat tat cct ggc gag tgg ttc gag gga ggg gcc acg 451

Val Thr Trp Asn Asp Tyr Pro Gly Glu Trp Phe Glu Gly Gly Ala Thr

105 110 115

acg gaa tca gaa aaa cag gac aag atc aat act ttt aga aac ctg ttg 499

Thr Glu Ser Glu Lys Gln Asp Lys Ile Asn Thr Phe Arg Asn Leu Leu

120 125 130

ggt tca gac gta gca ctc ttt ttg gtt gat ggg caa agg ctt cat gac 547

Gly Ser Asp Val Ala Leu Phe Leu Val Asp Gly Gln Arg Leu His Asp

135 140 145

tat gcc gat gat gag gag cgc tat ctt acg tac ctt ttt gat agc ttc 595

Tyr Ala Asp Asp Glu Glu Arg Tyr Leu Thr Tyr Leu Phe Asp Ser Phe

150 155 160 165

act gaa tcc ttg agc caa atc aaa gag gcg att ttg gag gat ggt aca 643

Thr Glu Ser Leu Ser Gln Ile Lys Glu Ala Ile Leu Glu Asp Gly Thr

170 175 180

ccg ctc caa cag ttc cct cgc atc tgg gtg att gct ttg tct aaa gct 691

Pro Leu Gln Gln Phe Pro Arg Ile Trp Val Ile Ala Leu Ser Lys Ala

185 190 195

gat tta tagccggatc tcacggttac agaattcgaa 727

Asp Leu

<210>114

<211>199

<212>PRT

<213>谷氨酸棒状杆菌

<400>114

Met Arg Cys Ile Asn Lys Thr Val Asp Gln Gln Ile Ala Leu Phe Gly

1 5 10 15

Gln Ser Gly Ser Gly Lys Thr Ala Leu Leu Cys Ser Phe Tyr Gly Thr

20 25 30

Ala Arg Glu Ser Ser Gln Glu Asp Val Lys Leu Phe Glu Ile Ser Ala

35 40 45

Glu Asp Asp Arg His Thr Glu Leu Met Arg Leu Tyr Leu Gly Met Arg

50 55 60

Asp Asp Ser Leu Phe Pro Pro Ala Asn Arg Phe Glu Ser Lys Asn Thr

65 70 75 80

Val Phe Ser Leu Lys Gln Lys Gly Val Pro Ile Lys Glu Ala Arg Lys

85 90 95

Ala Asp Gln Val Arg Val Thr Trp Asn Asp Tyr Pro Gly Glu Trp Phe

100 105 110

Glu Gly Gly Ala Thr Thr Glu Ser Glu Lys Gln Asp Lys Ile Asn Thr

115 120 125

Phe Arg Asn Leu Leu Gly Ser Asp Val Ala Leu Phe Leu Val Asp Gly

130 135 140

Gln Arg Leu His Asp Tyr Ala Asp Asp Glu Glu Arg Tyr Leu Thr Tyr

145 150 155 160

Leu Phe Asp Ser Phe Thr Glu Ser Leu Ser Gln Ile Lys Glu Ala Ile

165 170 175

Leu Glu Asp Gly Thr Pro Leu Gln Gln Phe Pro Arg Ile Trp Val Ile

180 185 190

Ala Leu Ser Lys Ala Asp Leu

195

<210>115

<211>2155

<212>DNA

<213>谷氨酸棒状杆菌

<220>

<221>CDS

<222>(101)..(2125)

<223>RXA04279

<400>115

cttgatatga cccgaacacc acacatcaca aattgaatcg gtatcctttg gggtattagt 60

ttccgtttta acgacacgac ttgcgaggag tcttaaaata atg gcg ttc tcc gta 115

Met Ala Phe Ser Val

1 5

gag atg ccc gag ctg ggc gaa tca gta acc gaa ggc acg atc acc cag 163

Glu Met Pro Glu Leu Gly Glu Ser Val Thr Glu Gly Thr Ile Thr Gln

10 15 20

tgg ttg aag tct gtt ggt gac act gtt gag gta gat gag ccg ttg ctc 211

Trp Leu Lys Ser Val Gly Asp Thr Val Glu Val Asp Glu Pro Leu Leu

25 30 35

gag gtc tca act gac aag gtc gac acc gag att ccc tct cct gtc gcc 259

Glu Val Ser Thr Asp Lys Val Asp Thr Glu Ile Pro Ser Pro Val Ala

40 45 50

ggt gtc atc cta gag att aag gct gaa gag gat gac acc gtc gac gtc 307

Gly Val Ile Leu Glu Ile Lys Ala Glu Glu Asp Asp Thr Val Asp Val

55 60 65

ggc ggt gtc att gca ata atc ggc gat gct gat gag act cct gcc aac 355

Gly Gly Val Ile Ala Ile Ile Gly Asp Ala Asp Glu Thr Pro Ala Asn

70 75 80 85

gaa gct cct gcc gac gag gca cca gct cct gcc gaa gag gaa gaa cca 403

Glu Ala Pro Ala Asp Glu Ala Pro Ala Pro Ala Glu Glu Glu Glu Pro

90 95 100

gtt aag gaa gag cca aag aag gag gca gct cct gaa gct cca gca gca 451

Val Lys Glu Glu Pro Lys Lys Glu Ala Ala Pro Glu Ala Pro Ala Ala

105 110 115

act ggc gcc gca acc gat gtg gaa atg cca gaa ctc ggc gaa tcc gtc 499

Thr Gly Ala Ala Thr Asp Val Glu Met Pro Glu Leu Gly Glu Ser Val

120 125 130

acc gaa ggc acc att acc cag tgg ctc aag gct gtc ggc gac acc gtc 547

Thr Glu Gly Thr Ile Thr Gln Trp Leu Lys Ala Val Gly Asp Thr Val

135 140 145

gaa gta gac gaa cca ctt ctt gag gtc tcc acc gac aag gtc gac acc 595

Glu Val Asp Glu Pro Leu Leu Glu Val Ser Thr Asp Lys Val Asp Thr

150 155 160 165

gaa atc cca tcc cca gta gca ggc acc atc gtg gag atc ctt gca gac 643

Glu Ile Pro Ser Pro Val Ala Gly Thr Ile Val Glu Ile Leu Ala Asp

170 175 180

gaa gac gac acc gtc gac gtc ggc gca gtc atc gcc cgc atc ggt gac 691

Glu Asp Asp Thr Val Asp Val Gly Ala Val Ile Ala Arg Ile Gly Asp

185 190 195

gca aac gca gct gca gca cct gcc gaa gag gaa gca gct cct gcc gaa 739

Ala Asn Ala Ala Ala Ala Pro Ala Glu Glu Glu Ala Ala Pro Ala Glu

200 205 210

gag gaa gaa cca gtt aag gaa gag cca aag aag gag gca gct cct gaa 787

Glu Glu Glu Pro Val Lys Glu Glu Pro Lys Lys Glu Ala Ala Pro Glu

215 220 225

gct cca gca gca act ggc gcc gca acc gat gtg gaa atg cca gaa ctc 835

Ala Pro Ala Ala Thr Gly Ala Ala Thr Asp Val Glu Met Pro Glu Leu

230 235 240 245

ggc gaa tcc gtc acc gaa ggc acc att acc cag tgg ctc aag gct gtc 883

Gly Glu Ser Val Thr Glu Gly Thr Ile Thr Gln Trp Leu Lys Ala Val

250 255 260

ggc gac acc gtc gaa gta gac gaa cca ctt ctt gag gtc tcc acc gac 931

Gly Asp Thr Val Glu Val Asp Glu Pro Leu Leu Glu Val Ser Thr Asp

265 270 275

aag gtc gac acc gaa atc cca tcc cca gta gca ggc acc atc gtg gag 979

Lys Val Asp Thr Glu Ile Pro Ser Pro Val Ala Gly Thr Ile Val Glu

280 285 290

atc ctt gca gac gaa gac gac acc gtc gac gtc ggc gca gtc atc gcc 1027

Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly Ala Val Ile Ala

295 300 305

cgc atc ggt gac gca aac gca gct gca gca cct gcc gaa gag gaa gca 1075

Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala Glu Glu Glu Ala

310 315 320 325

gct cct gcc gaa gag gag gaa cca gtt aag gaa gag cca aag aag gaa 1123

Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu Pro Lys Lys Glu

330 335 340

gag ccc aag aag gaa gag ccc aag aag gaa gca gct act aca cct gct 1171

Glu Pro Lys Lys Glu Glu Pro Lys Lys Glu Ala Ala Thr Thr Pro Ala

345 350 355

gcg gca tcc gca act gtg tcc gct tct ggc gac aac gtt cca tac gtc 1219

Ala Ala Ser Ala Thr Val Ser Ala Ser Gly Asp Asn Val Pro Tyr Val

360 365 370

acc cca ctg gtg cgc aag ctt gct gaa aag cac ggc gtt gac ttg aac 1267

Thr Pro Leu Val Arg Lys Leu Ala Glu Lys His Gly Val Asp Leu Asn

375 380 385

acc gtg acc ggt acc ggt atc ggt ggc cgt atc cgc aag cag gat gtt 1315

Thr Val Thr Gly Thr Gly Ile Gly Gly Arg Ile Arg Lys Gln Asp Val

390 395 400 405

ttg gct gct gcg aac ggc gag gct gca cct gct gag gct gct gct cct 1363

Leu Ala Ala Ala Asn Gly Glu Ala Ala Pro Ala Glu Ala Ala Ala Pro

410 415 420

gtt tcc gct tgg tcc act aag tct gtt gac cct gag aag gct aag ctc 1411

Val Ser Ala Trp Ser Thr Lys Ser Val Asp Pro Glu Lys Ala Lys Leu

425 430 435

cgt ggt acc act cag aag gtc aac cgc atc cgt gag atc acc gcg atg 1459

Arg Gly Thr Thr Gln Lys Val Asn Arg Ile Arg Glu Ile Thr Ala Met

440 445 450

aag acc gtc gag gct ctg cag att tct gct cag ctc acc cag ctg cac 1507

Lys Thr Val Glu Ala Leu Gln Ile Ser Ala Gln Leu Thr Gln Leu His

455 460 465

gag gtc gat atg act cgc gtt gct gag ctg cgt aag aag aac aag ccc 1555

Glu Val Asp Met Thr Arg Val Ala Glu Leu Arg Lys Lys Asn Lys Pro

470 475 480 485

gcg ttc atc gag aag cac ggt gtg aac ctc act tac ctg cca ttc ttc 1603

Ala Phe Ile Glu Lys His Gly Val Asn Leu Thr Tyr Leu Pro Phe Phe

490 495 500

gtg aag gca gtt gtc gag gct ttg gtt tcc cat cca aac gtc aac gcg 1651

Val Lys Ala Val Val Glu Ala Leu Val Ser His Pro Asn Val Asn Ala

505 510 515

tct ttc aac gcg aag acc aag gag atg acc tac cac tcc tcc gtt aac 1699

Ser Phe Asn Ala Lys Thr Lys Glu Met Thr Tyr His Ser Ser Val Asn

520 525 530

ctc tcc atc gct gtt gat acc cca gct ggt ctg ttg acc cca gtc att 1747

Leu Ser Ile Ala Val Asp Thr Pro Ala Gly Leu Leu Thr Pro Val Ile

535 540 545

cac gat gct cag gat ctc tcc atc cca gag atc gca aag gca att gtt 1795

His Asp Ala Gln Asp Leu Ser Ile Pro Glu Ile Ala Lys Ala Ile Val

550 555 560 565

gac ctg gct gat cgt tca cgc aac aac aag ctg aag cca aac gat ctg 1843

Asp Leu Ala Asp Arg Ser Arg Asn Asn Lys Leu Lys Pro Asn Asp Leu

570 575 580

tcc ggt ggc acc ttc acc atc acc aac att ggt tct gaa ggc gca ctg 1891

Ser Gly Gly Thr Phe Thr Ile Thr Asn Ile Gly Ser Glu Gly Ala Leu

585 590 595

tct gat acc cca atc ctg gtt cca cca cag gct ggc atc ttg ggc acc 1939

Ser Asp Thr Pro Ile Leu Val Pro Pro Gln Ala Gly Ile Leu Gly Thr

600 605 610

ggc gcg atc gtg aag cgt cca gtt gtc atc acc gag gat gga att gat 1987

Gly Ala Ile Val Lys Arg Pro Val Val Ile Thr Glu Asp Gly Ile Asp

615 620 625

tcc atc gcg atc cgt cag atg gtc ttc cta cca ctg acc tac gac cac 2035

Ser Ile Ala Ile Arg Gln Met Val Phe Leu Pro Leu Thr Tyr Asp His

630 635 640 645

cag gtt gta gat ggc gca gat gct ggt cgc ttc ctg acc acc atc aag 2083

Gln Val Val Asp Gly Ala Asp Ala Gly Arg Phe Leu Thr Thr Ile Lys

650 655 660

gac cgc ctt gag acc gct aac ttc gaa ggc gat ctg cag ctc 2125

Asp Arg Leu Glu Thr Ala Asn Phe Glu Gly Asp Leu Gln Leu

665 670 675

taagatctct gcaagttaaa accgccactc 2155

<210>116

<211>675

<212>PRT

<213>谷氨酸棒状杆菌

<400>116

Met Ala Phe Ser Val Glu Met Pro Glu Leu Gly Glu Ser Val Thr Glu

1 5 10 15

Gly Thr Ile Thr Gln Trp Leu Lys Ser Val Gly Asp Thr Val Glu Val

20 25 30

Asp Glu Pro Leu Leu Glu Val Ser Thr Asp Lys Val Asp Thr Glu Ile

35 40 45

Pro Ser Pro Val Ala Gly Val Ile Leu Glu Ile Lys Ala Glu Glu Asp

50 55 60

Asp Thr Val Asp Val Gly Gly Val Ile Ala Ile Ile Gly Asp Ala Asp

65 70 75 80

Glu Thr Pro Ala Asn Glu Ala Pro Ala Asp Glu Ala Pro Ala Pro Ala

85 90 95

Glu Glu Glu Glu Pro Val Lys Glu Glu Pro Lys Lys Glu Ala Ala Pro

100 105 110

Glu Ala Pro Ala Ala Thr Gly Ala Ala Thr Asp Val Glu Met Pro Glu

115 120 125

Leu Gly Glu Ser Val Thr Glu Gly Thr Ile Thr Gln Trp Leu Lys Ala

130 135 140

Val Gly Asp Thr Val Glu Val Asp Glu Pro Leu Leu Glu Val Ser Thr

145 150 155 160

Asp Lys Val Asp Thr Glu Ile Pro Ser Pro Val Ala Gly Thr Ile Val

165 170 175

Glu Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly Ala Val Ile

180 185 190

Ala Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala Glu Glu Glu

195 200 205

Ala Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu Pro Lys Lys

210 215 220

Glu Ala Ala Pro Glu Ala Pro Ala Ala Thr Gly Ala Ala Thr Asp Val

225 230 235 240

Glu Met Pro Glu Leu Gly Glu Ser Val Thr Glu Gly Thr Ile Thr Gln

245 250 255

Trp Leu Lys Ala Val Gly Asp Thr Val Glu Val Asp Glu Pro Leu Leu

260 265 270

Glu Val Ser Thr Asp Lys Val Asp Thr Glu Ile Pro Ser Pro Val Ala

275 280 285

Gly Thr Ile Val Glu Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val

290 295 300

Gly Ala Val Ile Ala Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro

305 310 315 320

Ala Glu Glu Glu Ala Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu

325 330 335

Glu Pro Lys Lys Glu Glu Pro Lys Lys Glu Glu Pro Lys Lys Glu Ala

340 345 350

Ala Thr Thr Pro Ala Ala Ala Ser Ala Thr Val Ser Ala Ser Gly Asp

355 360 365

Asn Val Pro Tyr Val Thr Pro Leu Val Arg Lys Leu Ala Glu Lys His

370 375 380

Gly Val Asp Leu Asn Thr Val Thr Gly Thr Gly Ile Gly Gly Arg Ile

385 390 395 400

Arg Lys Gln Asp Val Leu Ala Ala Ala Asn Gly Glu Ala Ala Pro Ala

405 410 415

Glu Ala Ala Ala Pro Val Ser Ala Trp Ser Thr Lys Ser Val Asp Pro

420 425 430

Glu Lys Ala Lys Leu Arg Gly Thr Thr Gln Lys Val Asn Arg Ile Arg

435 440 445

Glu Ile Thr Ala Met Lys Thr Val Glu Ala Leu Gln Ile Ser Ala Gln

450 455 460

Leu Thr Gln Leu His Glu Val Asp Met Thr Arg Val Ala Glu Leu Arg

465 470 475 480

Lys Lys Asn Lys Pro Ala Phe Ile Glu Lys His Gly Val Asn Leu Thr

485 490 495

Tyr Leu Pro Phe Phe Val Lys Ala Val Val Glu Ala Leu Val Ser His

500 505 510

Pro Asn Val Asn Ala Ser Phe Asn Ala Lys Thr Lys Glu Met Thr Tyr

515 520 525

His Ser Ser Val Asn Leu Ser Ile Ala Val Asp Thr Pro Ala Gly Leu

530 535 540

Leu Thr Pro Val Ile His Asp Ala Gln Asp Leu Ser Ile Pro Glu Ile

545 550 555 560

Ala Lys Ala Ile Val Asp Leu Ala Asp Arg Ser Arg Asn Asn Lys Leu

565 570 575

Lys Pro Asn Asp Leu Ser Gly Gly Thr Phe Thr Ile Thr Asn Ile Gly

580 585 590

Ser Glu Gly Ala Leu Ser Asp Thr Pro Ile Leu Val Pro Pro Gln Ala

595 600 605

Gly Ile Leu Gly Thr Gly Ala Ile Val Lys Arg Pro Val Val Ile Thr

610 615 620

Glu Asp Gly Ile Asp Ser Ile Ala Ile Arg Gln Met Val Phe Leu Pro

625 630 635 640

Leu Thr Tyr Asp His Gln Val Val Asp Gly Ala Asp Ala Gly Arg Phe

645 650 655

Leu Thr Thr Ile Lys Asp Arg Leu Glu Thr Ala Asn Phe Glu Gly Asp

660 665 670

Leu Gln Leu

675

Claims

1.分离的核酸分子，其编码的多肽所具有的氨基酸序列在每种情况下均涉及表1/第2列，其中在表1/第4列所示氨基酸位置处的核酸分子所编码的蛋白原氨基酸与表1/第5列相同行所示的特定氨基酸不同。

2.如权利要求1所述的分离的核酸分子，其中在表1/第4列中所示氨基酸位置处的核酸分子编码表1/第6列相同行所示的氨基酸。

3.载体，其包含至少一个如权利要求1所述的核酸序列。

4.宿主细胞，其用至少一种如权利要求3所述的载体转染。

5.如权利要求4所述的宿主细胞，其中所述核酸分子的表达调节所述细胞精细化学品的生产率。

6.制备精细化学品的方法，其包括培养已用至少一种如权利要求3所述的载体转染的细胞，从而产生精细化学品。

7.如权利要求6所述的方法，其中精细化学品是氨基酸。

8.如权利要求7所述的方法，其中所述氨基酸是赖氨酸。