CN1631210A - Feedstuff and healthy milk containing biological active selenium and production method thereof - Google Patents
Feedstuff and healthy milk containing biological active selenium and production method thereof Download PDFInfo
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Abstract
The invention provides a selenium-rich natural hygienic dairy product, wherein the newly screened bacterial is utilized to ferment the inorganic selenium, so as to transform into safe, highly effective feed addictive containing biologically active selenium. Milk with high selenium content can be produced through feeding the cows in milk with the additive.
Description
Technical field
The present invention relates to a kind of natural selenium-rich health care milk and utilize modern biotechnology to produce the method for natural selenium-rich health-care milk.
Background technology
Selenium is the trace element of needed by human, people's blood selenium level immediate parties's health status.Modern medicine study shows that selenium is a kind of powerful antioxidant in the human body, also has the absorption of regulating vitamin A, C, E etc. in the human body.Scarce selenium and cancer, cardiovascular and cerebrovascular diseases, diabetes, AIDS and people's agings etc. are closely related.In recent years, studies show that in a large number both at home and abroad that selenium has " target inhibitory or killing effect " to cancer cell,, cause research, the great upsurge in production that the whole world contains the selenium medicine and contains the selenium health products therefrom so be called as " anticancer king ".But, show through deep scientific research, be not that all selenium compounds all have effect of drugs and health-care effect, the material (comprising selenoprotein, selenoenzyme and seleno-amino acids) that only is converted into biologically active selenium just has good treatment, health-care effect.
Chinese patent application 94111703.0 discloses a kind of natural minerals feed that is combined by clinoptilolite, montmorillonite, hydromica, dolomite, calcite, opal, quartz, chlorite, sericite, carbonaceous, and selenium content is 9～18mg/kg in the feed.Add 3～8% to becoming in cow or every daily ration of growing cattle; in scarce selenium or low selenium area; can not only promote the metabolism of milk cow; and can make milk yield improve 5～10%; the growing cattle weightening finish improves 15%; and make trace elements such as being rich in selenium, zinc, strontium, calcium in the milk, and safe in utilization, have no side effect.
Chinese patent application 97106199.8 discloses a kind of fresh milk and goods thereof that are rich in organic selenium, it is characterized in that containing by feeding the selenium vinasse and makes mammal produce the fresh milk finished product or be 3-250 μ g/L as selenium content in the fresh milk of dairy produce raw material.Be the pollution-free food that integrates nutrition, health care, health, replenished the organic selenium that is easy to absorb to human body when feed, approach is reasonable, helps the developing ecology industry.
Chinese patent application 98120442.2 discloses a kind of selenium-rich milk product, raw material is dairy produce, pure natural organic selenium, organic selenium content range is 5～100 μ g in the every 100ml milk or in the 100g milk powder, this product contains abundant vitamin and trace elements of selenium, product can significantly improve the activity of interior superoxide dismutase of human body and glutathione peroxidase, has to alleviate oxygen radical to functions such as the infringement of human body and the processes that delays senility.
Chinese patent application 00122955.9 discloses a kind of Bioconverted selenium-enriched milk and production method thereof, it is the selenoaminoacid additive that in the concentrated feed of ox, sheep, adds doses, by ox, sheep domestic animal bulk absorption, the milk selenium content that conversion is produced is that 3～6 times of common milk are called Bioconverted selenium-enriched milk.These milk selenium content 20～30 microgram/kilograms meet in the state food selenium sanitary standard (≤30 microgram/kilogram) of limiting the quantity of.The selenoaminoacid additive is that the hydrochlorate and the chelating amino acids of inorganic selenium element forms.Its production cost is low, and rich selenium milk selenium content is stable.This selenium is to exist with organic selenium form that human body very easily absorbs, and is to mend the dairy produce that selenium is mended other nutrient simultaneously again.
The feed of containing inorganic selenium for the milk cow feeding because the milk cow absorption is limited, is difficult to obtain satisfactory selenium-rich milk product; In the prior art disclosed contain the hydrochlorate of selenium vinasse or inorganic selenium element and the absorption of organic selenium in milk cow that chelating amino acids forms also undesirable, therefore, the exploitation natural selenium enriched milk develops the most popular selenium health beverages that contains and has far-reaching social effect.
Summary of the invention
The object of the present invention is to provide a kind of feed that contains biologically active selenium, this feed is rich in nutrition and biologically active selenium, is easy to behind the animal edible absorb, and can improve immunity, premunition and the output of animal self on the one hand; Be rich in biologically active selenium in the milk of animal, egg or the meat on the other hand, have health care function after the people is edible.
Another object of the present invention is to provide a kind of natural selenium-rich health-care milk that contains biologically active selenium, this health-care milk selenium content height, and this a large amount selenium is organic selenium that human body easily absorbs.As a kind of novel milk product, have nutritious, safe in utilization, have the many-sided superior functions such as splendid health-care effect that improve immunologic function, build up health, reduce the disease invasion concurrently.
A further object of the present invention is to provide the method that adopts the above-mentioned feed that contains biologically active selenium to prepare the natural selenium-rich health-care milk, and this method can access the high natural milk of selenium content.
To achieve these goals, the technical solution used in the present invention is: a kind of feed addictive or feed that contains biologically active selenium, this feed addictive or feed are made up of biologically active selenium and animal feed, the content of biologically active selenium is calculated as 500-1200mg/kg with selenium in the feed addictive, and to be calculated as the described biologically active selenium of 1-1.5mg/kg with selenium be that inorganic selenium carries out the biologically active selenium that bio-transformation obtains through yeast to the content of biologically active selenium in the feed.
Animal feed wherein includes but not limited to the concentrated feed product that animal is commonly used, is mainly that volume is big, fibre composition content low (crude fiber content is less than 18% in the dry), feed that digestible nutrient content is high.The cow concentrate supplement of setting feedstuffs such as real class, amylaceous root, stem tuber melon and fruit class (potato class, beet), industrial by-product category (corn starch residue, DDGS, brewer's grain etc.), yeast class, grease class, cottonseed and preparing by a certain percentage as grass family seed, pulse family seed, puffed soybean, bran, grass-seed by multiple feedstuff; And the cereal crops, cereal crops byproduct and the goods that contain forage grass; And the natural animal byproduct, as fish meal, Bei Fen etc.
Animal concentrated feed of the present invention can also be disclosed other other feeds in the prior art, as:
Disclosed method and the product that utilizes agricultural crop straw to produce the fowl poultry kind concentrated feed as base-material among the CN92109988.6.Mainly utilizing the cellulosic ability of hypha of edible fungus decomposition and inversion, is base-material with stalk class crops, doses certain amount of urea, gypsum and rice bran, through mixing, fermentation, send out bacterium, sweat is produced the feed stuff of the high nutrition of a kind of fowl poultry kind again.Consist of straw 81-95%, rice bran 10-15%, urea 3-4%, gypsum 0.5-1%, edible fungi liquid strain is the 10-20% of above-mentioned total proportioning.
Disclosed method and the product that utilizes roughage to cook major ingredient production concentrated feed among the CN92111863.5.With grass, stalks base-material 81-86.5%, rice bran, wheat bran class auxiliary material 9-15%, urea 3.5-4%, gypsum 0.5%, edible fungi liquid strain amount is the 20-40% pulverizing back batch mixing of above-mentioned compost gross dry weight, through compost fermentation, compost inoculation edible mushroom, compost ferment again, compost inoculates edible mushroom, send out bacterium good technology such as dispatch from the factory, reach the effect of materials such as cellulose, hemicellulose, lignin in decomposition and inversion hay, the stalks roughage, thereby make it to be transformed into high nutritious extract feed.
The method of disclosed processing concentrated feed by using complex enzyme to treat plant straw and corresponding product among the CN95105933.5.The method of disclosed processing concentrated feed by using complex enzyme to treat distillers ' grains and corresponding product among the CN95105932.7.
A kind of preparation method who contains the feed addictive or the feed of biologically active selenium comprises the steps:
(4) photosynthetic bacteria is fermented in culture medium;
(5) adding inorganic selenium salt in zymotic fluid continues to cultivate;
(6) material absorbs with concentrated feed after being converted into peony, obtains containing the feed addictive or the feed of biologically active selenium;
Making the feed addictive of making contain selenium when the addition of inorganic selenium salt is (in Se) 500-1200mg/kg, the time, obtain the Se-enriched feedstuff additive, can be mixed with all feeds as required, make the Se-enriched feedstuff of various Se contents, the addition that also can be concentrated feed is to make the content of final biologically active selenium be calculated as 1-1.5mg/kg with selenium.
Inorganic selenium salt described in the said process is one or more the combination in sodium selenite, calcium selenite, selenous acid cobalt or the selenium dioxide.Concentrated feed as previously mentioned, preferred dregs of beans, wheat bran, the dish dregs of rice, black soya bean, oily chaff, cotton dregs cereal crops or other crops, grain or other farming byproducts and goods, the cereal crops that contain forage grass, cereal crops byproduct and goods; And the natural animal byproduct, as fish meal, Bei Fen etc.
Photosynthetic bacteria of the present invention is red pseudomonas (Rhodopseudomonas) or red spirillum (Rhodospiriliuml), preferred color red pseudomonas (Rhodopseudomonas palustris, Rps.Rutila), have a liking for a kind of or its combination in sulphur red pseudomonas (Rps.Sulfidophila), the Green color rhodopseudomonas (Rps.Viridis).Color red pseudomonas (the Rhodopseudomonas palustris in the red pseudomonas particularly, Rps.Rutila), can be used for feed fermentation with one of bacterial classification for what country announced, the selenium element is had powerfully transform, accumulation ability, and safe in utilization, do not produce environmental pollution.The consumption of fungi is the 5-10wt% of culture medium, and cultivation temperature is 25 ℃-30 ℃.Preferably in heliogreenhouse, cultivate under the illumination.
Fermentation and the time of cultivating are 2-5 days, preferably ferment 2-3 days.
Described culture medium is 0.1-1g yeast extract, 1-10g sodium acetate, 0.1-5g amine-oxides, 0.1-5g potassium dihydrogen phosphate, 0.1-3g magnesia, 1-6g sodium acid carbonate, during use it is dissolved in the 1000ml distilled water.
Preferred culture medium is 0.3g yeast extract, 5g sodium acetate, 1g amine-oxides, 1g potassium dihydrogen phosphate, 0.5g magnesia, when the 3g sodium acid carbonate uses it is dissolved in the 1000ml distilled water.
Yeast wherein is brewer's yeast, candida utili, product protein Saccharomyces globosus or is fit to other commercially available saccharomycete of the present invention.
The culture medium stirring and dissolving is after boiling sterilization, cooling inoculate red pseudomonas.
A kind of natural selenium-rich health-care milk that contains biologically active selenium is that the milk that rose to get milk cow in 10 days can obtain the natural selenium enriched milk that Se content can reach 30-40ng/ml with the direct feeding cow of feed that contains above-mentioned biologically active selenium.It is 31-40ng/ml that preferred natural selenium enriched milk gets Se content.
The preparation method who contains the natural selenium-rich health-care milk of biologically active selenium of the present invention is: earlier inorganic selenium is fermented, make inorganic selenium be converted into a kind of feed addictive that contains biologically active selenium, utilize this kind to contain the selenium additive for microbe feedstuff then and be mixed with a kind of direct feeding cow of milk cow forage that contains biologically active selenium, milk cow is that the output Se content can reach the high selenium composition milk greater than 30-40ng/ml, the natural selenium-rich health-care milk of meaning after absorbing active bio selenium.As a kind of novel milk product, have nutritious, safe in utilization, have many-sided superior functions such as splendid health-care effect concurrently.
Specifically, with containing the direct feeding cow of feed of above-mentioned biologically active selenium, the milk that rose to get milk cow in 10 days can obtain Se content can reach natural selenium enriched milk greater than 30-40ng/ml.The selenium milk that contains of milk cow output is processed, as asepticize, lactic fermentationization and be dried to milk powder, made diversified natural selenium-rich dairy produce.
The present invention utilizes photosynthetic bacterias such as red pseudomonas or red spirillum, through liquid fermentation, make inorganic selenium reach bio-transformation, make efficient, the safe selenium additive for microbe feedstuff that contains, utilize this kind to contain the preparation of selenium additive for microbe feedstuff and animal concentrated feed again and contain the direct feeding cow of selenium milk cow forage, the selenium milk that contains of milk cow output is processed (comprising asepticize, lactic fermentationization, and be dried to milk powder) and made diversified natural selenium-rich dairy produce.
The present invention mainly has the following advantages:
1. the selenium additive for microbe feedstuff that contains that makes after transforming of the inorganic selenium of Jia Ruing is a kind of safety, efficient additive, and is without any side effects to milk cow.
2. the hypertoxicity and the difficult world-famous puzzle that improves of milk selenium of inorganic selenium have been captured.Milk Bos anti-young animal, very low to the absorptivity of inorganic selenium, the reason of the world do not have so far natural selenium enriched milk listing that Here it is.And after adopting the biologically active selenium forage additive of the present invention's preparation, the selenium element can directly be entered in the milk by little intestinal absorption with the composition of selenoprotein, so but the higher natural selenium enriched milk of output Se content.
3. contain not only output natural selenium-rich health care milk of selenium additive for microbe feedstuff but milk cow feeds that the present invention produces, and can effectively prevent mastitis for milk cows, improve immunity, build up health, resist the effect of multiple disease.
Describe the present invention in detail below in conjunction with the drawings and specific embodiments.
Description of drawings:
Fig. 1 is the preparation technology figure of biologically active selenium feed of the present invention.
Be specific embodiments of the invention below, photosynthetic bacteria is provided by Institute of Micro-biology of the Chinese Academy of Sciences, red pseudomonas (Rhodopseudomonas) wherein, color red pseudomonas (Rhodopseudomonas palustris particularly, Rps.Rutila), yeast is the commercially available prod, see that the Ministry of Agriculture announces No. 244, production unit is that Huizhou curry favour scenery with hills and waters is given birth to biological products Co., Ltds (credit number 2002-1256), and microbial product factory of Zhujiang River aquatic products research institute also produces this product.
Embodiment 1
1. the preparation of biologically active selenium
In 1000ml distilled water, add 0.3g candida utili yeast extract, 5g sodium acetate, 1g amine-oxides, 1g potassium dihydrogen phosphate, 0.5g magnesia, 3g sodium acid carbonate, boiling sterilization after the stirring and dissolving, the ratio that is cooled to after 32 ℃ in 5wt% inserts color red pseudomonas (Rhodopseudomonas palustris Rps.Rutila).Put 25 ℃-26 ℃ of heliogreenhouses and cultivate after 3 days, add sodium selenite, its amount is (in Se) 800mg/kg for making the feed addictive of making contain selenium, continues to cultivate 5 days, and material changes into peony gradually, shows ripe.Sampling detects, and viable count is hundred million/ml of 10-11.Promptly make and contain the selenium additive for microbe feedstuff with wheat bran absorption, drying.Admix direct feed milk cow in the lactating cow feed.When Se content in the milk cow forage was 1-1.5mg/kg, selenium content can reach 30-50ng/ml in the milk of output, improved 1-2 doubly than the Se content (15ng/ml) of ordinary milk, was a kind of natural selenium-rich health care milk of preciousness.Can obtain dairy produces such as sour milk, milk powder, milk sheet after the deep processing.
Embodiment 2
In 1000ml distilled water, add 1g yeast extract (saccharomyces cerevisiae Brewer ' Sreast), the 3g sodium acetate, the 5g amine-oxides, the 3g potassium dihydrogen phosphate, 0.1g magnesia, the 1g sodium acid carbonate, boiling sterilization after the stirring and dissolving, fall that wet to insert red spirillum bacterium numbering in the ratio of 10wt% after 32 ℃ be 0192 (Rhodospiriliuml is totally 1 strain), the red pseudomonas bacterial classification, putting 29 ℃-30 ℃ of heliogreenhouses cultivated after 2 days, add the selenous acid cobalt, its amount is 1200mg/kg for making the feed addictive of making contain selenium for (in Se), continue to cultivate 3 days, material changes into peony gradually, shows ripe.Sampling detects, and viable count generally can reach hundred million/ml of 19-20.Promptly make and contain the selenium additive for microbe feedstuff with the absorption of the dish dregs of rice, drying.Admix direct feed milk cow in the lactating cow feed.When Se content in the milk cow forage was 1-1.5mg/kg, selenium content can reach 30-50ng/ml in the milk of output (liquid milk), improved 1-2 doubly than the Se content (15ng/ml) of ordinary milk, was a kind of natural selenium-rich health care milk of preciousness.Can obtain dairy produces such as sour milk, milk powder, milk sheet after the deep processing.
Embodiment 3
In 1000ml distilled water, add 0.1g yeast extract (producing the protein Saccharomyces globosus), the 1g sodium acetate, 0.1g amine-oxides, the 5g potassium dihydrogen phosphate, 3g magnesia, the 10g sodium acid carbonate, boiling sterilization after the stirring and dissolving, fall wet after 32 ℃ the ratio in 8wt% insert red pseudomonas bacterial classification (Rhodopseudomonas spheroides Rps.Globiformis), putting 28 ℃-29 ℃ of daylight moist chambers cultivated after 2.5 days, add calcium selenite or selenium dioxide, its amount contains selenium for (in Se) 500mg/kg concentration for making the feed addictive of making, continue to cultivate 3 days, material changes into peony gradually, shows ripe.Sampling detects, and viable count generally can reach hundred million/ml of 13-15.Promptly make and contain the selenium additive for microbe feedstuff with wheat bran absorption, drying.Admix direct feed milk cow in the lactating cow feed.When Se content in the milk cow forage was 1-1.5mg/kg, selenium content can reach 30-50ng/ml in the milk of output, improved 1-2 doubly than the Se content (15ng/ml) of ordinary milk, was a kind of natural selenium-rich health care milk of preciousness.Can obtain dairy produces such as sour milk, milk powder, milk sheet after the deep processing.
Claims (9)
1. a feed addictive or feed that contains biologically active selenium, this feed addictive or feed are made up of biologically active selenium and animal feed, the content of biologically active selenium is calculated as 500-1200mg/kg with selenium in the feed addictive, the content of biologically active selenium is calculated as 1-1.5mg/kg with selenium in the feed, and described biologically active selenium is that inorganic selenium carries out the biologically active selenium that bio-transformation obtains through yeast.
2. feed addictive according to claim 1 or feed, animal feed wherein is an animal concentrated feed product commonly used, comprising: dregs of beans, the dish dregs of rice, black soya bean, oily chaff, cotton dregs cereal crops or other crops, grain or other farming byproducts and goods, the cereal crops that contain forage grass, cereal crops byproduct and goods; The natural animal byproduct is as disclosed other feeds in fish meal, shellfish powder or the prior art.
3. claim 1 or the 2 described preparation methods that contain the feed addictive or the feed of biologically active selenium comprise the steps:
(1) photosynthetic bacteria is fermented in culture medium;
(2) adding inorganic selenium salt in zymotic fluid continues to cultivate;
(3) material is converted into after the peony and absorbs with concentrated feed, and the content that obtains selenium is calculated as the feed addictive of biologically active selenium of 500-1200mg/kg or selenium with selenium content is calculated as the feed of 1-1.5mg/kg with selenium.
4. the preparation method of feed addictive according to claim 3 or feed, inorganic selenium salt wherein is one or more the combination in sodium selenite, calcium selenite, selenous acid cobalt or the selenium dioxide.
5. the preparation method of feed addictive according to claim 4 or feed, photosynthetic bacteria bacterium wherein is red pseudomonas or red spirillum, preferred color red pseudomonas (Rhodopseudomonas palustris, Rps.Rutila), have a liking for a kind of or its combination in sulphur red pseudomonas (Rps.Sulfidophila), the Green color rhodopseudomonas (Rps.Viridis), preferred especially red pseudomonas, consumption is the 5-10wt% of culture medium.
6. the preparation method of feed addictive according to claim 5 or feed, its cultivation temperature are 25 ℃-30 ℃, and fermentation and the time of cultivating are 2-5 days, preferably cultivate under the illumination in heliogreenhouse, ferment 2-3 days.
7. the preparation method of feed addictive according to claim 3 or feed, described culture medium is 0.1-1g yeast extract, 1-10g sodium acetate, 0.1-5g amine-oxides, 0.1-5g potassium dihydrogen phosphate, 0.1-3g g magnesia, 1-6g sodium acid carbonate, during use it is dissolved in the 1000ml distilled water; Preferred culture medium is 0.3g yeast extract, 5g sodium acetate, 1g amine-oxides, 1g potassium dihydrogen phosphate, 0.5g magnesia, when the 3g sodium acid carbonate uses it is dissolved in the 1000ml distilled water.
8. a natural selenium-rich health-care milk that contains biologically active selenium is that the milk that rose to get milk cow in 10 days can obtain the natural selenium enriched milk that Se content can reach 30-40ng/ml with the direct feeding cow of feed that contains above-mentioned biologically active selenium.Preferred natural selenium enriched milk gets the 31-40ng/ml of Se content committee.
9. adopt claim 1 or 2 described feeds or feed addictive to prepare the method for natural selenium-rich health-care milk: earlier inorganic selenium to be fermented, make inorganic selenium be converted into a kind of feed addictive that contains biologically active selenium, utilize this kind to contain the selenium additive for microbe feedstuff then and be mixed with a kind of direct feeding cow of milk cow forage that contains biologically active selenium, milk cow is that the output Se content can reach the high selenium composition milk greater than 30-40ng/ml after absorbing active bio selenium, and tool is a milk powder.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101313729B (en) * | 2008-06-20 | 2011-03-16 | 田福平 | Organic biological feedstuff additive containing nano-selenium, zinc oxide, preparation and use method thereof |
| CN104171327A (en) * | 2014-07-29 | 2014-12-03 | 余姚市婉珍五金厂 | Selenium-rich Chinese herbal medicine fermented beef cattle feed |
| CN104489246A (en) * | 2014-12-12 | 2015-04-08 | 韦文峰 | Selenium-enriched microbial feed additive for dairy cow and preparation method thereof |
| CN105558353A (en) * | 2016-02-04 | 2016-05-11 | 王植 | Feed additive and preparation method thereof |
| CN105613421B (en) * | 2016-02-04 | 2018-07-06 | 北京康迪源科技有限公司 | Active selenium biological feedstuff, selenium-rich broiler chicken and laying hen and its production method |
| CN109666607A (en) * | 2018-12-29 | 2019-04-23 | 吴云 | A kind of preparation method of biological active selenium bacterium solution, biological active selenium bacterium solution, selenium-rich rice and its application |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107410493A (en) * | 2017-05-08 | 2017-12-01 | 田丙申 | A kind of preparation method of selenium-rich milk |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5726586A (en) * | 1980-07-18 | 1982-02-12 | Toshiaki Murata | Preparation of vegetable multienzyme through propagation in small amount |
| CN1076595C (en) * | 1994-06-21 | 2001-12-26 | 太原康强生物技术开发公司 | Producing animal feed additive using microbe and its manufacturing method |
| TW565432B (en) * | 1999-08-24 | 2003-12-11 | Chang-Yi Jeng | Method for producing Selenium-containing cow or goat milk |
| CN1282421C (en) * | 2002-04-22 | 2006-11-01 | 梁学俭 | Production process of selenium-rich milk and relevant concentrated feed |
| CN100512666C (en) * | 2003-08-18 | 2009-07-15 | 汤珍山 | Selenium containing microopganic additive for forage and its preparation |
-
2003
- 2003-12-24 CN CNB2003101230708A patent/CN1307895C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101313729B (en) * | 2008-06-20 | 2011-03-16 | 田福平 | Organic biological feedstuff additive containing nano-selenium, zinc oxide, preparation and use method thereof |
| CN104171327A (en) * | 2014-07-29 | 2014-12-03 | 余姚市婉珍五金厂 | Selenium-rich Chinese herbal medicine fermented beef cattle feed |
| CN104489246A (en) * | 2014-12-12 | 2015-04-08 | 韦文峰 | Selenium-enriched microbial feed additive for dairy cow and preparation method thereof |
| CN105558353A (en) * | 2016-02-04 | 2016-05-11 | 王植 | Feed additive and preparation method thereof |
| CN105613421B (en) * | 2016-02-04 | 2018-07-06 | 北京康迪源科技有限公司 | Active selenium biological feedstuff, selenium-rich broiler chicken and laying hen and its production method |
| CN109666607A (en) * | 2018-12-29 | 2019-04-23 | 吴云 | A kind of preparation method of biological active selenium bacterium solution, biological active selenium bacterium solution, selenium-rich rice and its application |
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| CN1307895C (en) | 2007-04-04 |
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