CN107410493A - A kind of preparation method of selenium-rich milk - Google Patents

A kind of preparation method of selenium-rich milk Download PDF

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CN107410493A
CN107410493A CN201710316514.1A CN201710316514A CN107410493A CN 107410493 A CN107410493 A CN 107410493A CN 201710316514 A CN201710316514 A CN 201710316514A CN 107410493 A CN107410493 A CN 107410493A
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selenium
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田丙申
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/1522—Inorganic additives, e.g. minerals, trace elements; Chlorination or fluoridation of milk; Organic salts or complexes of metals other than natrium or kalium; Calcium enrichment of milk
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • Food Science & Technology (AREA)
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Abstract

The present invention relates to a kind of preparation method of selenium-rich milk, comprise the following steps：Enrichment culture first is carried out to the bacterium solution containing photosynthetic bacteria, is then isolated and purified to obtain single bacterium colony；RCVBN fluid nutrient mediums are taken, are inoculated with single bacterium colony by 5 8wt% inoculum concentration, and add Na2SeO3Solution, it is (1 4) × 10 that selenium content, which is made,‑5Mol/L selenium-rich culturing base, 28 30 DEG C are subsequently placed in, illumination cultivation is carried out under 2000lx intensities of illumination, bioactivity Se-enriched feedstuff additive is obtained, is compounded with animal feed, obtain Organic Selenium feed；With Organic Selenium feed breeding milk cow, you can obtain selenium-rich milk.The present invention solves the milk cow technical barrier low to selenium element absorptivity, overcomes cow rumen " refusing selenium effect " to inorganic selenium additive salt, effectively increases the Se content in fresh milk, practicable approach is provided for production rich selenium milk.

Description

A kind of preparation method of selenium-rich milk
Technical field
The invention belongs to technical field of bioengineering, and in particular to one kind utilizes modern biotechnology production live body Organic Selenium The method of health care dairy produce.
Background technology
Selenium is one of indispensable trace element of Animal nutrition, and its physiological function is mainly improved body immunity, clear Except free radical, cardioprotection and liver, prevent from producing toxicant etc. in vivo, but the distributed pole of selenium is uneven in nature, There is 72% regional selenium deficiency in China, wherein 29% area belongs to serious selenium deficiency.
Selenium is divided into inorganic selenium, Organic Selenium and active selenium three major types.Inorganic selenium is usually sodium selenite, and price is very cheap, but Larger toxicity, and be not easy to be absorbed, be not suitable for humans and animals and use；Organic Selenium exists typically in the form of selenomethionine, follows Methionine metabolism approach is metabolized, and is participated in the synthesis of albumen, can be stored, absorb in tissue, be absorbed by the body, but due to absorbing Rate is low, and excretion is incomplete, easily remains in internal, harm is brought to body；Active selenium is divided into vegetable active selenium and active animal Selenium, vegetable active selenium be crops from the soil of selenium-rich element by nanometer root absorption technology, selenium is absorbed in vivo, warp The whole growth course of crops is crossed, by photosynthesis and the biotransformation of crops itself, mineral matter and organic shape The selenium element of state, change into selenomethionine for being easily absorbed by the human body and being metabolized etc.；Active animal selenium is that animal edible is raised containing selenium Selenium is absorbed in vivo by material, by the metabolism of animal body, selenium is converted into active selenium, is present in meat, egg, milk.It is living Property selenium is easily absorbed by the human body, and is had no toxic side effect, can be more rapid and better absorbed by the body.
In recent years, although people study crowd selenium-supply of the health food containing selenium to selenium deficiency area, for example high-selenium eggs, to green The research and industrialization of the natural active selenium milk of safety of color are there is not yet report.Milk, it is human consumption's absorptivity highest food One of product, and the food that numerous consumers are conventional.In milk, in addition to dietary fiber, the complete nutrients containing needed by human body Matter, an adult drink 500ml milk, can obtain 15-17g high-quality proteins daily, and 600 obtained ㎎ calcium are equivalent to daily requirement 80%, the 11% of the daily caloric requirement of human body can be met.But Se content is less than 5 μ g/ ㎏ in milk, selenium reinforcing is carried out to milk It is the shortcut of a selenium-supply, not only improves absorption of human body, is also more easy to be received by consumer.
In the prior art, P.Aspila produces selenium-rich milk with the silage grass feeding cow through spraying selenium culture.Gong Wei, field It is glad by adding Na in milk cow forage2SeO3Produce selenium-rich milk.Application No. CN201410212385.8 patent of invention is led to Cross selenium-rich stalk, the cow concentrate containing humic acid, effective microorganisms(EM), greenfeed and feed ox production selenium-rich milk；Application No. CN200710140550.3 patent of invention produces selenium-rich edible fungi residue feed by the leftover bits and pieces of selenium-enriched edible mushroom, and it is rich to feed Cow product Selenium milk.But by the converted Se-enriched feedstuff of plant, Se content is relatively low, and milk cow is to the absorptivity of such selenium element Low, it is atomic to result in Se content in resulting milk, and preferable se complementary effect can not be obtained by quoting such milk containing selenium.
Therefore, how traditional milk is converted to rich in the active animal selenium ox that can be more rapid and better absorbed by the body Milk, production enhancement food are the goals of the invention of the present invention.
The content of the invention
In view of this, it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of preparation of selenium-rich milk Method, the present invention solve that milk cow is low to selenium element absorptivity, the atomic technical barrier of Se content in milk, and the present invention is sharp twice With modern biotechnology, inorganic selenium is changed into Organic Selenium, cow rumen is overcome and inorganic selenium additive salt " is refused selenium to make With ", Organic Selenium, without directly can be absorbed through cud conversion by intestinal wall, can effectively improve fresh milk after milk cow alimentary canal is entered In Se content, provide practicable approach for large-scale production high-quality natural selenium enriched milk.
To realize object above, the present invention adopts the following technical scheme that：
A kind of preparation method of selenium-rich milk, comprises the following steps：
(1) enrichment culture first is carried out to the bacterium solution containing photosynthetic bacteria, enrichment culture thing is obtained, by the enrichment culture thing Isolated and purified to obtain single bacterium colony；
(2) RCVBN fluid nutrient mediums are taken, are inoculated with the single bacterium colony by 5-8wt% inoculum concentration, and add Na2SeO3It is molten Liquid, selenium content is made as 1 × 10-5mol/L-4×10-5Mol/L selenium-rich culturing base, 28-30 DEG C is subsequently placed in, 2000lx light According to illumination cultivation is carried out under intensity, when fluid nutrient medium is changed into peony, bioactivity Se-enriched feedstuff additive is obtained；
(3) the bioactivity Se-enriched feedstuff additive is compounded with animal feed, obtains Organic Selenium feed；
(4) with the Organic Selenium feed breeding milk cow, you can obtain selenium-rich milk.
Preferably：Enrichment culture described in step (1) is to add the water sample containing photosynthetic bacteria in enriched medium, in , 7-10d is cultivated under 2000lx intensities of illumination, is then transferred in new enriched medium, repeat culture 2-3 times, i.e., by 30-32 DEG C Obtain enrichment culture thing.
Further：The photosynthetic bacteria is red pseudomonas or red spirillum.
Further：The enriched medium includes sodium acetate 3.2-4.8g, sodium lactate 2.4-3.6g, ammonium sulfate 0.8- 1.2g, peptone 0.16-0.24g, yeast extract 0.32-0.48g, distilled water 800-1200mL.
Further：The enriched medium includes sodium acetate 4.0g, sodium lactate 3.0g, ammonium sulfate 1.0g, albumen Peptone 0.2g, yeast extract 0.4g, distilled water 1000mL.
Preferably：Isolated and purified described in step (1) to be detested using double-layer plate and the self-control of pyrogallic acid oxygen adsorption method The method that oxygen bag combines carries out pure culture to photosynthetic bacteria.
Further：The pure culture comprises the following steps：
S1：Enrichment culture thing is taken, is coated with plating dilutions, uses double-layer plate, in illumination box, 2000lx illumination Cultivate 7-10d, the red bacterium colony thalline line separation of picking；
S2：Anaerobism bag is made by oneself using pyrogallic acid oxygen adsorption method, and illumination cultivation is to there is red single bacterium colony to come out, then picking Single bacterium colony separates 2-3 times in flat lining out, then carries out aerobic dark culturing as 27-29 DEG C of constant incubator, until going out Now without the single bacterium colony of living contaminants.
Preferably：Na described in step (2)2SeO3The addition manner of solution is batch feeding selenium-supply, last time Na2SeO3The addition time of solution is not later than the exponential phase of the single bacterium colony.
The present inventor is in research process discovery, different plus the photosynthetic strain of selenium time effects growth and the conversion of selenium Rate, if add before the exponential phase (between 12-24h) sodium selenite can obtain it is relatively good turn selenium result, if mistake Early to add, cell concentration is than relatively low, and also than relatively low, growth is easily suppressed vigor, is added too late, decline phase thalline largely declines Die, be also unfavorable for the conversion of selenium.And batch selenium-supply can improve selenium switching-in rate and be transferred to the absolute magnitude of selenium, this is mainly utilized In growth course, bacterial concentration incrementally increases thalline, and selenium also progressively reduces to the coercive of thalline, and it is sub- now to add a part Sodium selenate and culture medium, total selenium amount, but the no too much influence of growth to thalline can be lifted in certain period of time, is advantageous to Turn the raising of selenium amount and cell concentration, by batch feeding selenium-supply, thalline is transferred to the absolute magnitude of selenium and add 39.25%, adopt Selenium-rich finished product organic selenium content is measured with 3,3- diaminobenzidines colorimetric method, total selenium amount is 938ug/g, Organic Selenium Conversion ratio it is stable more than 90%.
Preferably：Selenium content is 800-1200mg/kg in step (3) the Organic Selenium feed.
Preferably：Selenium content is 30-40ng/ml in selenium-rich milk described in step (4).
RCVBN fluid nutrient mediums are mainly used in rich product culture, separation and preserve strain, and its formula is：DL malic acid 4g, phosphorus Sour hydrogen dipotassium and potassium phosphate buffer 10mmoL, epsom salt 120mg, ammonium sulfate 1g, calcium chloride dihydrate 75mg, second two Amine tetraacethyl sodium 20mg, biotin 15ug, vitamin B1 1mg, niacin 1mg, minor metallic element reservoir 1mL, distilled water 1000mL, PH 6.8.
Beneficial effects of the present invention：
The present invention solves that milk cow is low to selenium element absorptivity, the atomic technical barrier of Se content in milk, utilizes twice Modern biotechnology, inorganic selenium is changed into Organic Selenium, overcome cow rumen and inorganic selenium additive salt " is refused selenium to make With ", Organic Selenium, without directly can be absorbed through cud conversion by intestinal wall, can effectively improve fresh milk after milk cow alimentary canal is entered In Se content, provide practicable approach for large-scale production high-quality natural selenium enriched milk.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below Detailed description.Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Base Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned Other embodiment, belong to the scope that the present invention is protected.
Embodiment 1
A kind of preparation method of selenium-rich milk, comprises the following steps：
(1) water sample containing red pseudomonas is added in enriched medium, cultivated under 30 DEG C, 2000lx intensities of illumination 7d, it is then transferred in new enriched medium, (wherein enriched medium includes sodium acetate 3.2g, sodium lactate 3.6g, sulfuric acid Ammonium 0.8g, peptone 0.24g, yeast extract 0.32g, distilled water 1200mL) culture 2 times is repeated, enrichment culture thing is obtained, by described in Enrichment culture thing double-layer plate dilution spread, in illumination box, 2000lx illumination cultivation 7d, the red bacterium colony bacterium of picking Body line separation；Anaerobism bag is made by oneself using pyrogallic acid oxygen adsorption method, and illumination cultivation is to there is red single bacterium colony to come out, then picking Single bacterium colony separates 2 times in flat lining out, then carries out aerobic dark culturing as 27 DEG C of constant incubators, until not having The single bacterium colony of living contaminants；
(2) RCVBN fluid nutrient mediums are taken (containing DL malic acid 4g, dipotassium hydrogen phosphate and potassium phosphate buffer 10mmoL, epsom salt 120mg, ammonium sulfate 1g, calcium chloride dihydrate 75mg, sodium ethylene diamine tetracetate 20mg, biotin 15ug, Vitamin B1 1mg, niacin 1mg, minor metallic element reservoir 1mL, distilled water 1000mL, its PH are 6.8), by 6wt%'s Inoculum concentration is inoculated with the single bacterium colony, and batch adds Na2SeO3Solution carries out selenium-supply, forms final selenium content as 1 × 10- 5Mol/L selenium-rich culturing base, and last time Na2SeO3The addition time of solution is not later than the logarithmic growth of the single bacterium colony Phase, 28 DEG C are placed in, illumination cultivation is carried out under 2000lx intensities of illumination, when fluid nutrient medium is changed into peony, obtains biology Active Se-enriched feedstuff additive；
(3) the bioactivity Se-enriched feedstuff additive is compounded with animal feed, having obtained selenium content is 800mg/kg machine selenium feeds；
(4) with the Organic Selenium feed breeding milk cow, you can obtain the selenium-rich milk that selenium content is 30ng/ml.
Embodiment 2
A kind of preparation method of selenium-rich milk, comprises the following steps：
(1) water sample containing red pseudomonas is added in enriched medium, cultivated under 30 DEG C, 2000lx intensities of illumination 8d, it is then transferred in new enriched medium, (wherein enriched medium includes sodium acetate 4.0g, sodium lactate 3.0g, sulfuric acid Ammonium 1.0g, peptone 0.2g, yeast extract 0.4g, distilled water 1000mL) culture 3 times is repeated, enrichment culture thing is obtained, by the richness Collect culture double-layer plate dilution spread, in illumination box, 2000lx illumination cultivation 7d, the red bacterium colony thalline of picking Line separation；Anaerobism bag is made by oneself using pyrogallic acid oxygen adsorption method, and illumination cultivation is to there is red single bacterium colony to come out, then picking list Bacterium colony separates 3 times in flat lining out, then carries out aerobic dark culturing as 27 DEG C of constant incubators, until occurring not miscellaneous The single bacterium colony of bacterium pollution；
(2) RCVBN fluid nutrient mediums are taken (containing DL malic acid 4g, dipotassium hydrogen phosphate and potassium phosphate buffer 10mmoL, epsom salt 120mg, ammonium sulfate 1g, calcium chloride dihydrate 75mg, sodium ethylene diamine tetracetate 20mg, biotin 15ug, Vitamin B1 1mg, niacin 1mg, minor metallic element reservoir 1mL, distilled water 1000mL, its PH are 6.8), by 5wt%'s Inoculum concentration is inoculated with the single bacterium colony, and batch adds Na2SeO3Solution carries out selenium-supply, forms final selenium content as 4 × 10- 5Mol/L selenium-rich culturing base, and last time Na2SeO3The addition time of solution is not later than the logarithmic growth of the single bacterium colony Phase, 30 DEG C are placed in, illumination cultivation is carried out under 2000lx intensities of illumination, when fluid nutrient medium is changed into peony, obtains biology Active Se-enriched feedstuff additive；
(3) the bioactivity Se-enriched feedstuff additive is compounded with animal feed, having obtained selenium content is 1200mg/kg machine selenium feeds；
(4) with the Organic Selenium feed breeding milk cow, you can obtain the selenium-rich milk that selenium content is 40ng/ml.
Embodiment 3
A kind of preparation method of selenium-rich milk, comprises the following steps：
(1) water sample containing red spirillum is added in enriched medium, 10d is cultivated under 32 DEG C, 2000lx intensities of illumination, It is then transferred in new enriched medium, (wherein enriched medium includes sodium acetate 4.8g, sodium lactate 2.4g, ammonium sulfate 1.2g, peptone 0.16g, yeast extract 0.48g, distilled water 800mL) culture 3 times is repeated, enrichment culture thing is obtained, by the richness Collect culture double-layer plate dilution spread, in illumination box, 2000lx illumination cultivation 10d, the red bacterium colony bacterium of picking Body line separation；Anaerobism bag is made by oneself using pyrogallic acid oxygen adsorption method, and illumination cultivation is to there is red single bacterium colony to come out, then picking Single bacterium colony separates 3 times in flat lining out, then carries out aerobic dark culturing as 29 DEG C of constant incubators, until not having The single bacterium colony of living contaminants；
(2) RCVBN fluid nutrient mediums are taken (containing DL malic acid 4g, dipotassium hydrogen phosphate and potassium phosphate buffer 10mmoL, epsom salt 120mg, ammonium sulfate 1g, calcium chloride dihydrate 75mg, sodium ethylene diamine tetracetate 20mg, biotin 15ug, Vitamin B1 1mg, niacin 1mg, minor metallic element reservoir 1mL, distilled water 1000mL, its PH are 6.8), by 8wt%'s Inoculum concentration is inoculated with the single bacterium colony, and batch adds Na2SeO3Solution carries out selenium-supply, forms final selenium content as 3 × 10- 5Mol/L selenium-rich culturing base, and last time Na2SeO3The addition time of solution is not later than the logarithmic growth of the single bacterium colony Phase, 30 DEG C are placed in, illumination cultivation is carried out under 2000lx intensities of illumination, when fluid nutrient medium is changed into peony, obtains biology Active Se-enriched feedstuff additive；
(3) the bioactivity Se-enriched feedstuff additive is compounded with animal feed, having obtained selenium content is 1000mg/kg Organic Selenium feeds；
(4) with the Organic Selenium feed breeding milk cow, you can obtain the selenium-rich milk that selenium content is 35ng/ml.
Organic Selenium feed effect in the embodiment of the present invention 2 is detected below：
The present invention feeds milk by the Organic Selenium feed and general feeds to milk cow hello various dose, discovery with organic feed Ox, can be decreased obviously its blood, serum and Liver MDA, red blood cell, hepatic tissue SOD vigor, GSH-Px vigor, The indexs such as total antioxidation level increase, and positive effect is better than the experimental group of feeding general feeds, and this is probably due to life Thing activity Se-enriched feedstuff additive plays inhibitory action by carotenoid to lipid peroxidation, and photosynthetic bacteria is as carrier When sodium selenite is converted into Organic Selenium, the active material of thalline in itself is also strengthened, and selenium is GSH-Px enzymes again Required part, therefore Organic Selenium feed just further improves the active energy of internal antioxidase compared with general feeds Power, make the effect of anti-grease peroxidating more notable.
The organic selenium content of bioactivity Se-enriched feedstuff additive is surveyed using 3,3- diaminobenzidines colorimetric method Fixed, total selenium amount is 938ug/g, and the conversion ratio of Organic Selenium is stable more than 90%.The Organic Selenium feed substantially increases milk cow pair The absorptivity of selenium element, Se content has been reached 40ng/ml, people eat such milk can obtain compared with Good se complementary effect.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.

Claims (10)

1. a kind of preparation method of selenium-rich milk, it is characterised in that comprise the following steps：
(1) enrichment culture first is carried out to the bacterium solution containing photosynthetic bacteria, obtains enrichment culture thing, the enrichment culture thing is carried out Isolate and purify to obtain single bacterium colony；
(2) RCVBN fluid nutrient mediums are taken, are inoculated with the single bacterium colony by 5-8wt% inoculum concentration, and add Na2SeO3Solution, system It is 1 × 10 into selenium content-5mol/L-4×10-5Mol/L selenium-rich culturing base, 28-30 DEG C is subsequently placed in, 2000lx intensities of illumination Lower carry out illumination cultivation, when fluid nutrient medium is changed into peony, obtain bioactivity Se-enriched feedstuff additive；
(3) the bioactivity Se-enriched feedstuff additive is compounded with animal feed, obtains Organic Selenium feed；
(4) with the Organic Selenium feed breeding milk cow, you can obtain selenium-rich milk.
2. preparation method according to claim 1, it is characterised in that enrichment culture described in step (1) is that will contain light The water sample for closing bacterium is added in enriched medium, and 7-10d is cultivated under 30-32 DEG C, 2000lx intensities of illumination, is then transferred to new Enriched medium in, repeat culture 2-3 times, produce enrichment culture thing.
3. preparation method according to claim 2, it is characterised in that the photosynthetic bacteria is red pseudomonas or red spiral shell Bacterium.
4. preparation method according to claim 2, it is characterised in that the enriched medium includes sodium acetate 3.2- 4.8g, sodium lactate 2.4-3.6g, ammonium sulfate 0.8-1.2g, peptone 0.16-0.24g, yeast extract 0.32-0.48g, distilled water 800-1200mL。
5. preparation method according to claim 2, it is characterised in that the enriched medium include sodium acetate 4.0g, Sodium lactate 3.0g, ammonium sulfate 1.0g, peptone 0.2g, yeast extract 0.4g, distilled water 1000mL.
6. preparation method according to claim 1, it is characterised in that isolated and purified described in step (1) as using double-deck The method that flat board and pyrogallic acid oxygen adsorption method self-control anaerobism bag combine carries out pure culture to photosynthetic bacteria.
7. preparation method according to claim 6, it is characterised in that the pure culture comprises the following steps：
S1：Enrichment culture thing is taken, is coated with plating dilutions, uses double-layer plate, in illumination box, 2000lx illumination cultivations 7-10d, the red bacterium colony thalline line separation of picking；
S2：Anaerobism bag is made by oneself using pyrogallic acid oxygen adsorption method, and illumination cultivation is to there is red single bacterium colony to come out, then picking single bacterium Fall and separated 2-3 times in flat lining out, then carry out aerobic dark culturing as 27-29 DEG C of constant incubator, until not having There is the single bacterium colony of living contaminants.
8. preparation method according to claim 1, it is characterised in that Na described in step (2)2SeO3The addition side of solution Formula is batch feeding selenium-supply, last time Na2SeO3The addition time of solution is not later than the exponential phase of the single bacterium colony.
9. preparation method according to claim 1, it is characterised in that selenium content is in step (3) the Organic Selenium feed 800-1200mg/kg。
10. preparation method according to claim 1, it is characterised in that selenium content in selenium-rich milk described in step (4) 30-40ng/ml。
CN201710316514.1A 2017-05-08 2017-05-08 A kind of preparation method of selenium-rich milk Pending CN107410493A (en)

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CN101063098A (en) * 2007-04-20 2007-10-31 重庆大学 Culture method for photosynthetic bacterium strain having hydrogen-generation specificity
CN101948768A (en) * 2010-08-20 2011-01-19 沈阳大学 Method for preparing photosynthetic bacteria for degrading 2-chlorophenol
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Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1307895C (en) * 2003-12-24 2007-04-04 北京精硒生物工程有限责任公司 Feedstuff and healthy milk containing biological active selenium and production method thereof
CN101063098A (en) * 2007-04-20 2007-10-31 重庆大学 Culture method for photosynthetic bacterium strain having hydrogen-generation specificity
CN101948768A (en) * 2010-08-20 2011-01-19 沈阳大学 Method for preparing photosynthetic bacteria for degrading 2-chlorophenol
CN103740615A (en) * 2013-12-26 2014-04-23 莱州市顺昌水产有限公司 Photosynthetic bacteria SC01 as well as fast cultivation method and application thereof

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Title
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Application publication date: 20171201