CN1432069A - 可降解的核酸探针和核酸检测方法 - Google Patents

可降解的核酸探针和核酸检测方法 Download PDF

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Publication number
CN1432069A
CN1432069A CN01810638A CN01810638A CN1432069A CN 1432069 A CN1432069 A CN 1432069A CN 01810638 A CN01810638 A CN 01810638A CN 01810638 A CN01810638 A CN 01810638A CN 1432069 A CN1432069 A CN 1432069A
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China
Prior art keywords
probe
nucleic acid
region
target
pair
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Pending
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CN01810638A
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English (en)
Chinese (zh)
Inventor
鲁埃尔·范阿塔
戴维·阿尔巴格里
迈克尔·L·伍德
彼得·C·陈
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Naxcor Inc
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Naxcor Inc
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Publication of CN1432069A publication Critical patent/CN1432069A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN01810638A 2000-04-18 2001-04-18 可降解的核酸探针和核酸检测方法 Pending CN1432069A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/551,305 US6573048B1 (en) 2000-04-18 2000-04-18 Degradable nucleic acid probes and nucleic acid detection methods
US09/551,305 2000-04-18

Publications (1)

Publication Number Publication Date
CN1432069A true CN1432069A (zh) 2003-07-23

Family

ID=24200719

Family Applications (1)

Application Number Title Priority Date Filing Date
CN01810638A Pending CN1432069A (zh) 2000-04-18 2001-04-18 可降解的核酸探针和核酸检测方法

Country Status (11)

Country Link
US (1) US6573048B1 (https=)
EP (1) EP1280938A2 (https=)
JP (1) JP2004501615A (https=)
KR (1) KR20030005289A (https=)
CN (1) CN1432069A (https=)
CA (1) CA2406096A1 (https=)
CZ (1) CZ20023749A3 (https=)
HU (1) HUP0301031A2 (https=)
MX (1) MXPA02010283A (https=)
PL (1) PL362928A1 (https=)
WO (1) WO2001079563A2 (https=)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102703584A (zh) * 2012-04-20 2012-10-03 中国人民解放军第四军医大学 自组装聚合分支dna探针的制备方法
CN106170564A (zh) * 2014-02-04 2016-11-30 欧凌科生物科技公司 基于杂交链式反应(hcr)的检测的邻近试验
CN109983125A (zh) * 2016-08-31 2019-07-05 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
CN110295235A (zh) * 2019-07-26 2019-10-01 合肥欧创基因生物科技有限公司 一种双标记探针及其在snp分型及肿瘤突变的应用
US12098425B2 (en) 2018-10-10 2024-09-24 Readcoor, Llc Three-dimensional spatial molecular indexing
US12203136B2 (en) 2020-08-17 2025-01-21 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US12559790B2 (en) 2020-01-29 2026-02-24 10X Genomics, Inc. Compositions and methods for analyte detection

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WO2002059137A1 (en) * 2000-11-03 2002-08-01 Isis Pharmaceuticals, Inc. Nuclease-based method for detecting and quantitating oligonucleotides
EP1513958B1 (en) 2002-06-14 2011-08-10 Gen-Probe Incorporated Compositions for detecting hepatitis b virus
US8575327B2 (en) * 2003-06-12 2013-11-05 Alnylam Pharmaceuticals, Inc. Conserved HBV and HCV sequences useful for gene silencing
EP2290071B1 (en) * 2004-05-28 2014-12-31 Asuragen, Inc. Methods and compositions involving microRNA
US20060078894A1 (en) * 2004-10-12 2006-04-13 Winkler Matthew M Methods and compositions for analyzing nucleic acids
CA2857881A1 (en) 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
JP4712814B2 (ja) * 2005-11-17 2011-06-29 独立行政法人科学技術振興機構 特定の塩基配列の標的核酸類を検出する方法、及び検出のための核酸類セット
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
EP2104734A2 (en) * 2006-12-08 2009-09-30 Asuragen, INC. Mir-20 regulated genes and pathways as targets for therapeutic intervention
WO2008073915A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Micrornas differentially expressed in leukemia and uses thereof
CA2671270A1 (en) * 2006-12-29 2008-07-17 Asuragen, Inc. Mir-16 regulated genes and pathways as targets for therapeutic intervention
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
JP2010529966A (ja) * 2007-06-08 2010-09-02 アシュラジェン インコーポレイテッド 治療的介入の標的としてｍｉＲ−３４によって調節される遺伝子および経路
US8361714B2 (en) * 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
US20090186015A1 (en) * 2007-10-18 2009-07-23 Latham Gary J Micrornas differentially expressed in lung diseases and uses thereof
US8071562B2 (en) 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
WO2009126726A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
US8258111B2 (en) * 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
WO2010056577A1 (en) * 2008-11-17 2010-05-20 Magic Technologies, Inc. Methods and compositions in particle-based detection of target molecules using linking molecules
US9469868B2 (en) * 2008-11-17 2016-10-18 Headway Technologies, Inc. Methods and compositions in particle-based detection of target molecules using covalent bond forming reactive pairs
WO2012112563A2 (en) 2011-02-16 2012-08-23 Magic Technologies, Inc. Methods and compositions for the target-localized anchoring of detectable label
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
US9738925B2 (en) * 2013-03-15 2017-08-22 Lyle J. Arnold Methods for immobilizing target nucleic acids utilizing combinatorial capture probes
JP6210484B2 (ja) * 2013-06-25 2017-10-11 国立大学法人北陸先端科学技術大学院大学 鎖交換された二重鎖オリゴヌクレオチドの製造方法
TWI710633B (zh) 2014-11-10 2020-11-21 美商阿尼拉製藥公司 Ｂ型肝炎病毒（ＨＢＶ）ｉＲＮＡ組成物及其用途方法
US11324820B2 (en) 2017-04-18 2022-05-10 Alnylam Pharmaceuticals, Inc. Methods for the treatment of subjects having a hepatitis b virus (HBV) infection
CA3106701A1 (en) 2018-08-13 2020-02-20 Alnylam Pharmaceuticals, Inc. Hepatitis b virus (hbv) dsrna agent compositions and methods of use thereof

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599303A (en) * 1983-12-12 1986-07-08 Hri Associates, Inc. Nucleic acid hybridization assay employing probes crosslinkable to target sequences
US4826967A (en) 1987-06-16 1989-05-02 Naxcor Psoralen-nucleoside adducts and method for their preparation
US5403711A (en) * 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
US6004826A (en) * 1988-07-20 1999-12-21 David Segev Repair-mediated process for amplifying and detecting nucleic acid sequences
US5082934A (en) 1989-04-05 1992-01-21 Naxcor Coumarin derivatives for use as nucleotide crosslinking reagents
US5767259A (en) * 1994-12-27 1998-06-16 Naxcor Oligonucleotides containing base-free linking groups with photoactivatable side chains
WO1994024120A1 (en) 1993-04-13 1994-10-27 Naxcor Non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents
US5616464A (en) 1994-12-27 1997-04-01 Naxcor Nucleic acid sequence detection employing amplification probes
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
AU724506B2 (en) 1995-11-03 2000-09-21 Naxcor Double-stranded conformational polymorphism analysis
CN1285879A (zh) 1998-01-09 2001-02-28 美国3M公司 可特异性酶切断裂的多核苷酸底物和测试方法
WO1999042616A1 (en) 1998-02-20 1999-08-26 Dade Behring Inc. Methods for determining concentrations of nucleic acids
US6124099A (en) * 1998-06-22 2000-09-26 The University Of Vermont And State Agricultural College Method for placing a photo-cross-linking agent at specific internal sites within the sequence of synthetic strands of ribonucleic acids
US6303799B1 (en) 1998-11-10 2001-10-16 Naxcor Polynucleotide crosslinking agents

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102703584A (zh) * 2012-04-20 2012-10-03 中国人民解放军第四军医大学 自组装聚合分支dna探针的制备方法
CN102703584B (zh) * 2012-04-20 2013-08-21 中国人民解放军第四军医大学 自组装聚合分支dna探针的制备方法
CN106170564A (zh) * 2014-02-04 2016-11-30 欧凌科生物科技公司 基于杂交链式反应(hcr)的检测的邻近试验
CN106170564B (zh) * 2014-02-04 2020-01-03 欧凌科生物科技公司 基于杂交链式反应(hcr)的检测的邻近试验
CN109983125A (zh) * 2016-08-31 2019-07-05 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
CN109983125B (zh) * 2016-08-31 2024-06-04 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
US12098425B2 (en) 2018-10-10 2024-09-24 Readcoor, Llc Three-dimensional spatial molecular indexing
CN110295235A (zh) * 2019-07-26 2019-10-01 合肥欧创基因生物科技有限公司 一种双标记探针及其在snp分型及肿瘤突变的应用
US12559790B2 (en) 2020-01-29 2026-02-24 10X Genomics, Inc. Compositions and methods for analyte detection
US12203136B2 (en) 2020-08-17 2025-01-21 Readcoor, Llc Methods and systems for spatial mapping of genetic variants

Also Published As

Publication number Publication date
CA2406096A1 (en) 2001-10-25
US6573048B1 (en) 2003-06-03
EP1280938A2 (en) 2003-02-05
KR20030005289A (ko) 2003-01-17
MXPA02010283A (es) 2003-05-23
WO2001079563A2 (en) 2001-10-25
WO2001079563A3 (en) 2002-09-19
PL362928A1 (en) 2004-11-02
CZ20023749A3 (cs) 2003-04-16
HUP0301031A2 (hu) 2003-07-28
JP2004501615A (ja) 2004-01-22

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