CN1426466A - 遗传沉默 - Google Patents

遗传沉默 Download PDF

Info

Publication number
CN1426466A
CN1426466A CN01808640A CN01808640A CN1426466A CN 1426466 A CN1426466 A CN 1426466A CN 01808640 A CN01808640 A CN 01808640A CN 01808640 A CN01808640 A CN 01808640A CN 1426466 A CN1426466 A CN 1426466A
Authority
CN
China
Prior art keywords
nucleotide sequence
cells
cell
gene
endogenous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN01808640A
Other languages
English (en)
Chinese (zh)
Inventor
M·W·格雷海姆
R·N·赖斯
K·M·墨菲
K·C·里德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BENETECH AUSTRALIA Ltd
Original Assignee
BENETECH AUSTRALIA Ltd
Department of Agriculture and Fisheries
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPQ6363A external-priority patent/AUPQ636300A0/en
Priority claimed from AUPR2700A external-priority patent/AUPR270001A0/en
Application filed by BENETECH AUSTRALIA Ltd, Department of Agriculture and Fisheries filed Critical BENETECH AUSTRALIA Ltd
Publication of CN1426466A publication Critical patent/CN1426466A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/18Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with another compound as one donor, and incorporation of one atom of oxygen (1.14.18)
    • C12Y114/18001Tyrosinase (1.14.18.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN01808640A 2000-03-17 2001-03-16 遗传沉默 Pending CN1426466A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
AUPQ6363 2000-03-17
AUPQ6363A AUPQ636300A0 (en) 2000-03-17 2000-03-17 Genetic silencing
AUPR2700 2001-01-24
AUPR2700A AUPR270001A0 (en) 2001-01-24 2001-01-24 Genetic silencing

Publications (1)

Publication Number Publication Date
CN1426466A true CN1426466A (zh) 2003-06-25

Family

ID=25646282

Family Applications (1)

Application Number Title Priority Date Filing Date
CN01808640A Pending CN1426466A (zh) 2000-03-17 2001-03-16 遗传沉默

Country Status (12)

Country Link
US (1) US20030182672A1 (https=)
EP (1) EP1272629A4 (https=)
JP (2) JP2003527856A (https=)
KR (1) KR20020097198A (https=)
CN (1) CN1426466A (https=)
AU (1) AU2001240375A1 (https=)
BR (1) BR0109269A (https=)
CA (1) CA2403162A1 (https=)
GB (1) GB2377221B (https=)
MX (1) MXPA02009069A (https=)
PL (1) PL358230A1 (https=)
WO (1) WO2001070949A1 (https=)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
WO1999049029A1 (en) 1998-03-20 1999-09-30 Benitec Australia Ltd Control of gene expression
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
EP2314700A1 (en) 1999-01-28 2011-04-27 Medical College of Georgia Research Institute, Inc Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
DE19956568A1 (de) 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
CN1378602A (zh) 1999-08-26 2002-11-06 卡尔根尼有限公司 含修饰的多不饱和脂肪酸的植物
US7067722B2 (en) 1999-08-26 2006-06-27 Monsanto Technology Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7531718B2 (en) 1999-08-26 2009-05-12 Monsanto Technology, L.L.C. Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7829693B2 (en) 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
DE10100586C1 (de) 2001-01-09 2002-04-11 Ribopharma Ag Verfahren zur Hemmung der Expression eines Ziegens
WO2003070918A2 (en) 2002-02-20 2003-08-28 Ribozyme Pharmaceuticals, Incorporated Rna interference by modified short interfering nucleic acid
US8273866B2 (en) 2002-02-20 2012-09-25 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SINA)
US8202979B2 (en) 2002-02-20 2012-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
US7423142B2 (en) 2001-01-09 2008-09-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
US7767802B2 (en) 2001-01-09 2010-08-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
US8546143B2 (en) 2001-01-09 2013-10-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
EP1229134A3 (en) 2001-01-31 2004-01-28 Nucleonics, Inc Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
US20050148530A1 (en) 2002-02-20 2005-07-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US7517864B2 (en) 2001-05-18 2009-04-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
WO2005078097A2 (en) 2004-02-10 2005-08-25 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING MULTIFUNCTIONAL SHORT INTERFERING NUCLEIC ACID (Multifunctional siNA)
US9994853B2 (en) 2001-05-18 2018-06-12 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
EP2345720A3 (en) 2001-07-12 2012-01-25 University of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
US10590418B2 (en) 2001-07-23 2020-03-17 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for RNAi mediated inhibition of gene expression in mammals
ES2386775T3 (es) 2001-07-23 2012-08-30 The Board Of Trustees Of The Leland Stanford Junior University Procedimientos y composiciones para la inhibición mediada por ARNi de la expresión génica en mamíferos
US20050037989A1 (en) * 2001-08-27 2005-02-17 Lewis David L. Inhibition of gene function by delivery of polynucleotide-based gene expression inhibitors to mammalian cells in vivo
US7745418B2 (en) 2001-10-12 2010-06-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting viral replication
DE10163098B4 (de) 2001-10-12 2005-06-02 Alnylam Europe Ag Verfahren zur Hemmung der Replikation von Viren
DE10202419A1 (de) 2002-01-22 2003-08-07 Ribopharma Ag Verfahren zur Hemmung der Expression eines durch eine Chromosomen-Aberration entstandenen Zielgens
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
AU2003207708A1 (en) 2002-02-20 2003-09-09 Sirna Therapeutics, Inc. Rna interference mediated inhibition of map kinase genes
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
AR039113A1 (es) 2002-03-21 2005-02-09 Monsanto Technology Llc Construcciones de acidos nucleicos y metodos para producir composiciones de aceites de semillas alteradas
US7566813B2 (en) 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
US7166771B2 (en) 2002-06-21 2007-01-23 Monsanto Technology Llc Coordinated decrease and increase of gene expression of more than one gene using transgenic constructs
US20040172667A1 (en) 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20050042646A1 (en) 2002-08-05 2005-02-24 Davidson Beverly L. RNA interference suppresion of neurodegenerative diseases and methods of use thereof
US20080274989A1 (en) 2002-08-05 2008-11-06 University Of Iowa Research Foundation Rna Interference Suppression of Neurodegenerative Diseases and Methods of Use Thereof
US20040241854A1 (en) 2002-08-05 2004-12-02 Davidson Beverly L. siRNA-mediated gene silencing
AU2003256615A1 (en) 2002-08-12 2004-02-25 New England Biolabs, Inc. Methods and compositions relating to gene silencing
US7956176B2 (en) 2002-09-05 2011-06-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7122361B2 (en) 2002-10-10 2006-10-17 Wyeth Compositions employing a novel human kinase
CA2503491A1 (en) 2002-10-24 2004-05-06 Wyeth Calcineurin-like human phosphoesterase
AU2003298689A1 (en) 2002-11-21 2004-06-18 Wyeth Methods for diagnosing rcc and other solid tumors
US7297525B2 (en) 2002-11-27 2007-11-20 Wyeth Composition employing a novel human kinase
WO2005009346A2 (en) * 2003-06-24 2005-02-03 Mirus Corporation Inhibition of gene function by delivery of polynucleotide-based gene expression inhibitors to mammalian cells in vivo
WO2005019440A1 (en) 2003-08-20 2005-03-03 Northern Sydney And Central Coast Area Health Service Methods for enhancing embryo viability
EP1672064A4 (en) * 2003-09-22 2006-12-20 Riken EFFICIENT METHOD FOR GENERATING AN INVERTED REPEAT DNA STRUCTURE
DE10351149A1 (de) * 2003-11-03 2005-06-30 Beiersdorf Ag Oligoribonukleotide zur Behandlung von unerwünschter Pigmentierung der Haut und der Haare durch RNA-Interferenz
WO2005062881A2 (en) 2003-12-24 2005-07-14 Transgenrx, Inc. Gene therapy using transposon-based vectors
AU2005238034A1 (en) 2004-04-23 2005-11-10 The Trustees Of Columbia University In The City Of New York Inhibition of hairless protein mRNA
US10508277B2 (en) 2004-05-24 2019-12-17 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
CA2576193A1 (en) 2004-08-03 2006-02-16 Biogen Idec Ma Inc. Taj in neuronal function
JP2008515458A (ja) 2004-10-13 2008-05-15 ユニバーシティ オブ ジョージア リサーチ ファウンデーション, インコーポレーテッド 線虫抵抗性の遺伝子組換え植物
CN101213301B (zh) 2005-05-31 2013-02-27 德福根有限公司 用于防治昆虫和蜘蛛类动物的RNAi
EP2980220A1 (en) 2005-09-20 2016-02-03 BASF Plant Science GmbH Improved methods controlling gene expression
US8093369B2 (en) 2005-10-11 2012-01-10 Ben Gurion University Of The Negev Research And Development Authority Ltd. Compositions for silencing the expression of VDAC1 and uses thereof
CA2631621C (en) 2005-11-29 2019-08-06 Cambridge Enterprise Limited Markers for breast cancer including rs2981582
CA2913655A1 (en) 2006-01-27 2007-08-09 Biogen Ma Inc. Nogo receptor antagonists
CN101421406B (zh) 2006-02-13 2016-08-31 孟山都技术有限公司 用于产生改变的种子油组成的核酸构建体和方法
FR2898908A1 (fr) 2006-03-24 2007-09-28 Agronomique Inst Nat Rech Procede de preparation de cellules aviaires differenciees et genes impliques dans le maintien de la pluripotence
AU2007276388A1 (en) 2006-07-21 2008-01-24 Silence Therapeutics Ag Means for inhibiting the expression of protein kinase 3
EP2124522B1 (en) 2007-02-05 2011-11-16 National University of Singapore Putative cytokinin receptor and methods for use thereof
PL2129680T3 (pl) 2007-03-21 2015-10-30 Brookhaven Science Ass Llc Łączone kompozycje cząsteczek antysensownych o strukturze spinki do włosów oraz sposoby modulacji ekspresji
WO2009129558A1 (en) 2008-04-24 2009-10-29 Newsouth Innovations Pty Limited Cyanobacteria saxitoxin gene cluster and detection of cyanotoxic organisms
US8093043B2 (en) 2008-06-04 2012-01-10 New York University β-TrCP1, β-TrCP2 and RSK1 or RSK2 inhibitors and methods for sensitizing target cells to apoptosis
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
WO2010036976A2 (en) 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
AR075549A1 (es) 2008-09-29 2011-04-20 Monsanto Technology Llc Evento transgenico de soja mon87705 y metodos para detectar el mismo
MY159849A (en) 2008-12-22 2017-02-15 Lek Pharmaceuticals Mammalian expression vector
EP2241627A1 (en) * 2009-04-17 2010-10-20 LEK Pharmaceuticals d.d. Mammalian expression vector
EP2417263B1 (en) 2009-04-09 2015-09-23 ProteoVec Holding L.L.C. Production of proteins using transposon-based vectors
WO2010139026A1 (en) 2009-06-05 2010-12-09 Centenary Institute Of Cancer Medicine And Cell Biology Therapeutic and diagnostic molecules
EP3000885B1 (en) 2009-12-18 2018-07-25 Arrowhead Pharmaceuticals, Inc. Organic compositions to treat hsf1-related diseases
JP2013519869A (ja) 2010-02-10 2013-05-30 ノバルティス アーゲー 筋肉成長のための方法および化合物
WO2012058210A1 (en) 2010-10-29 2012-05-03 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACIDS (siNA)
CA2860676A1 (en) 2012-01-09 2013-07-18 Novartis Ag Organic compositions to treat beta-catenin-related diseases
PL3265563T3 (pl) 2015-02-02 2021-09-13 Meiragtx Uk Ii Limited Regulacja ekspresji genów poprzez modulację alternatywnego splicingu za pośrednictwem aptamerów

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5272065A (en) * 1983-10-20 1993-12-21 Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US5208149A (en) * 1983-10-20 1993-05-04 The Research Foundation Of State University Of New York Nucleic acid constructs containing stable stem and loop structures
US5190931A (en) * 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US4766072A (en) * 1985-07-17 1988-08-23 Promega Corporation Vectors for in vitro production of RNA copies of either strand of a cloned DNA sequence
US5231020A (en) * 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5034323A (en) * 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5714323A (en) * 1991-08-30 1998-02-03 The University Of Medecine And Dentistry Of New Jersey Over expression of single-stranded molecules
CA2130454C (en) * 1992-02-19 2008-04-08 William G. Dougherty Production of viral resistant plants via introduction of untranslatable plus sense viral rna
DE4208107A1 (de) * 1992-03-13 1993-09-16 Bayer Ag Pseudorabies-virus (prv)-polynukleotide und ihre verwendung zur herstellung von virusresistenten eukaryotischen zellen
US5624803A (en) * 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
US5578716A (en) * 1993-12-01 1996-11-26 Mcgill University DNA methyltransferase antisense oligonucleotides
US5686649A (en) * 1994-03-22 1997-11-11 The Rockefeller University Suppression of plant gene expression using processing-defective RNA constructs
US6054299A (en) * 1994-04-29 2000-04-25 Conrad; Charles A. Stem-loop cloning vector and method
CA2262776C (en) * 1996-08-02 2005-03-08 Genesense Technologies, Inc. Antitumor antisense sequences directed against r1 and r2 components of ribonucleotide reductase
US5814500A (en) * 1996-10-31 1998-09-29 The Johns Hopkins University School Of Medicine Delivery construct for antisense nucleic acids and methods of use
AU2005299A (en) * 1997-12-20 1999-07-12 Genencor International, Inc. Fluidized bed matrix granule
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AUPP249298A0 (en) * 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
WO1999049029A1 (en) * 1998-03-20 1999-09-30 Benitec Australia Ltd Control of gene expression
NZ507093A (en) * 1998-04-08 2003-08-29 Commw Scient Ind Res Org Methods and means for reducing the phenotypic expression of a nucleic acid of interest in a plant
EP2314700A1 (en) * 1999-01-28 2011-04-27 Medical College of Georgia Research Institute, Inc Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US6423885B1 (en) * 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
GB9927444D0 (en) * 1999-11-19 2000-01-19 Cancer Res Campaign Tech Inhibiting gene expression
PT1309726E (pt) * 2000-03-30 2010-03-08 Whitehead Biomedical Inst Mediadores de interferência por rna específicos de sequência de rna
ATE507300T1 (de) * 2000-06-23 2011-05-15 Du Pont Rekombinante konstrukte und ihre verwendung bei der reduzierung der genexpression
US7109393B2 (en) * 2000-08-15 2006-09-19 Mendel Biotechnology, Inc. Methods of gene silencing using inverted repeat sequences

Also Published As

Publication number Publication date
JP2003527856A (ja) 2003-09-24
AU2001240375A1 (en) 2001-10-03
PL358230A1 (en) 2004-08-09
US20030182672A1 (en) 2003-09-25
JP2011229542A (ja) 2011-11-17
EP1272629A4 (en) 2004-12-22
GB2377221B (en) 2004-08-18
WO2001070949A1 (en) 2001-09-27
GB0224195D0 (en) 2002-11-27
CA2403162A1 (en) 2001-09-27
KR20020097198A (ko) 2002-12-31
GB2377221A (en) 2003-01-08
BR0109269A (pt) 2002-12-17
MXPA02009069A (es) 2004-04-05
EP1272629A1 (en) 2003-01-08

Similar Documents

Publication Publication Date Title
CN1426466A (zh) 遗传沉默
CN1231491C (zh) 哺乳动物端粒酶
CN1617929A (zh) siRNA表达系统和利用该系统制备功能性基因击倒细胞的方法
Liu et al. Generation of a triple‐gene knockout mammalian cell line using engineered zinc‐finger nucleases
CN1189562C (zh) 针对核糖核苷酸还原酶r1和r2组分的抗肿瘤反义序列
CN1167795C (zh) 肽、dna及抗体
CN1620508A (zh) 转基因生物
JP2017513510A (ja) ブタにおける多重遺伝子編集
CN1900319A (zh) 用双链rna抑制鉴定基因功能
CN1796556A (zh) 控制基因表达
CN1334878A (zh) 具有遍在染色质开放表达元件(ucoe)的聚核苷酸
CN1685063A (zh) 与基因沉默相关的方法和组合物
CN1789416A (zh) 含人抗体λ轻链基因的人类人工染色体
CN1612930A (zh) 抑制基因表达的方法
CN1491280A (zh) 滑膜细胞蛋白质
CN1906211A (zh) 新的肌肉生长调节物
EP3352567A1 (en) Genetically modified animals having increased heat tolerance
CN1620503A (zh) 细胞内制备单链dna
CN1665930A (zh) 具有RNAi效应的茎环RNA分子的表达系统
CN1977168A (zh) 治疗肺癌的组合物和方法
CN1276083C (zh) 用体内产生的ssDNA改变基因表达
CN1867672A (zh) 改良的siRNA分子和应用该改良的siRNA分子的抑制基因表达的方法
CN1812797A (zh) 双链核糖核酸在诱导细胞裂解中的应用
CN1602359A (zh) 靶向性载体的制备方法及其应用
CN1780912A (zh) 抑制细胞生长的组合物和方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Owner name: NONE

Free format text: FORMER OWNER: LANZHOU CITY, KUNSHI

Effective date: 20040604

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20040604

Address after: Queensland, Australia

Applicant after: Benetech Australia Ltd.

Address before: Queensland, Australia

Applicant before: Benetech Australia Ltd.

Co-applicant before: The Queensland Department of primary industries

C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication
REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1057061

Country of ref document: HK