CN1325098C - 一种提高免疫力的中药组合物及制备方法和质量控制方法 - Google Patents
一种提高免疫力的中药组合物及制备方法和质量控制方法 Download PDFInfo
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Abstract
Description
水平因素 | 煎煮时间(h) | 煎煮次数 | 煎煮用水量(倍数) |
123 | 0.751.01.5 | 123 | 578 |
方差来源 | 离均平方和 | 自由度 | 方差 | F值 | 显著性 |
ABCD | 16.6319.730.06670.0733 | 2222 | 8.3159.8650.03340.0367 | 226.57268.800.910 | 高度显著高度显著不显著 |
S总=36.50 |
组号 | 1 | 2 | 3 | SA2=1.7344SA3=1.8346S2 A2=3.0082S2 A3=3.3658F=S2 A2/S2 A3=0.8973F1-0.05(2,2)=19.0F<F1-0.05(2,2) |
A2A3 | 9.529.65 | 12.6512.75 | 12.3812.90 |
组号 | 1 | 2 | 3 | SB2=1.7344SB3=1.8346S2 B2=3.0082S2 B3=3.3658F=S2 B2/S2 B3=0.8973F1-0.05(2,2)=19.0F<F1-0.05(2,2) |
B2B3 | 9.6510.05 | 12.6512.38 | 12.7512.90 |
温度(℃) | 40 | 60 | 80 |
黄芪甲苷含量(mg/g) | 0.068 | 0.082 | 0.081 |
批号 | 970102 | 970301 | 970501 |
含量 | 0.404 | 0.408 | 0.407 |
组别 | 动物数 | 剂量(g/kg) | 肉芽肿重量(mg/100g体重) |
空白对照组低剂组高剂组药物对照组 | 8888 | -1.102.203.75 | 64.32±5.4555.43±6.61*△47.00±8.80**56.75±6.90*△ |
组别 | 动物数 | 剂量(g/kg) | 肉芽肿重量(mg/100g体重) |
空白对照组低剂组高剂组药物对照组 | 8888 | -1.102.203.75 | 332.59±22.15309.68 ±32.73*292.10±28.00**302.34±31.84* |
组别 | 动物数 | 剂量(g/kg) | 肿胀率 | ||||
30min | 1h | 2h | 3h | 4h | |||
空白对照组低剂组高剂组药物对照组 | 8888 | -1.102.203.75 | 60.91±23.0363.92±12.1169.22±14.1871.53±23.03 | 62.58±23.7659.79±14.88(4.46)56.99±21.72(8.93)71.96±23.65 | 55.66±21.2252.41±12.07(5.84)37.05±16.55(33.44)51.18±22.63 | 35.35±23.4331.93±16.01(9.07)24.38±16.93(31.03)30.35±11.12 | 28.28±16.8121.95±12.30(22.36)21.35±16.92(24.48)24.10±15.53 |
分组 | n | 剂量(g/kg) | 总抗氧化力(单位/ml) |
空白对照组造模组低剂量组高剂量组药物对照组 | 1010101010 | --2.5g/kg5.0g/kg7.5g/kg | 14.31±2.078.91±1.05△△10.32±1.23*13.08±1.96**12.67±1.73** |
分组 | n | 剂量(g/kg) | T-SOD(NU/mg蛋白) |
空白对照组造模对照组低剂量组高剂量组药物对照组 | 1010101010 | --2.5g/kg5.0g/kg7.5g/kg | 0.90±0.150.67±0.16△0.84±0.14*1.40±0.15**1.26±0.12** |
组别(n=10) | 剂量(g/kg) | 丙二醛含量(nM/ml) |
正常对照组造模组低剂组高剂组参苏丸组 | --2.55.07.5 | 9.35±1.5414.97±2.13△△12.86±1.78*10.77±0.99**11.23±1.46** |
组别 | n | 剂量(g/kg) | Na+,k+-ATP酶活力(μmolpi/mgprot/h) |
空白对照组造模对照组低剂量组高剂量组药物对照组 | 1010101010 | --2.55.07.5 | 40.09±5.8642.95 ±4.29△38.51±3.4436.18±5.80*36.56±4.56* |
组别 | N | 剂量(g/kg) | 存活时间(min) |
正常对照组低剂组高剂组药物对照组 | 10101010 | -2.55.07.5 | 88.50±25.13103.80±53.18113.10±26.16*108.00±41.39 |
组别 | n | 剂量(g/kg) | 生存时间(min) |
正常对照组低剂组高剂组药物对照组 | 991010 | -2.55.07.5 | 4.68±0.595.86±1.978.69±4.05*6.75±2.53 |
组别 | n | 剂量(g/kg) | 存活时间(min) |
正常对照组低剂组高剂组药物对照组 | 10101010 | -2.55.07.5 | 25.00±9.2128.80±10.4038.20±11.23*28.20±9.30 |
组别 | n | 剂量(g/kg) | 血清溶血素OD值 |
正常免疫组环磷酰胺组芪风固表低剂组芪风固表中剂组芪风固表高剂组阳性药物对照组 | 101010101010 | --2.55.010.015.0 | 1.42±0.140.82±0.14△△0.92±0.161.26±0.11*1.36±0.18**1.29±0.14** |
组别 | 动物数(只) | 剂量(g/kg) | C3b受体花环率(%) | C3b免疫复合物花环率(%) |
空白对照组模型组低剂组高剂组参苏丸组 | 1010101010 | --2.55.07.5 | 14.9±5.88.3±3.1△△9.7±4.513.3±4.2**13.4±5.7** | 8.9±3.216.7±5.4△△13.2±6.610.4±3.4**10.5±2.5** |
组别 | n | 剂量(g/kg) | 血中残留刚果红(μg/ml) |
正常对照组低剂组高剂组阳性药对照组 | 99109 | -2.55.07.5 | 5.03±1.414.22±1.123.40±0.68**4.21±0.98 |
组别 | n | 剂量(g/kg) | 溶菌酶含量(μg/ml) |
空白对照组模型组(环磷酰胺)低剂组高剂组阳性药对照组 | 1010101010 | --2.55.07.5 | 6.68±3.504.12±1.64△5.12±2.346.61±3.12*6.55±2.11* |
组别 | 动物数 | 剂量(g/kg) | 胸腺指数 | 脾指数 |
空白对照组模型组低剂组高剂组阳性药对照组 | 1010101010 | --2.55.07.5 | 2.71±0.731.68±0.71△2.00±0.992.77±0.79**2.06±0.54 | 3.72±1.012.98±0.673.02±0.583.83±0.86*3.58±0.63 |
组别 | 动物数 | 剂量(g/kg) | 干扰素效价(log4/ml) |
空白对照组低剂量组高剂量组 | 61111 | 5.0g/kg7.5g/kg | 没检出1.72±0.792.59±0.65 |
标准品 | 6.1 |
组别n=8 | 剂量(g/kg) | 气管酚红排泌量(μg/ml) |
空白对照组低剂量组高剂量组阳性药对照组 | 等体积生理盐水5g/kg7.5g/kg12.5gkg | 0.227±0.0500.362±0.1590.543±0.124**0.320±0.147 |
Claims (16)
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CN104027470A (zh) * | 2013-03-10 | 2014-09-10 | 高见 | 一种增强机体抵抗能力防治感冒的中药 |
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CN104982777A (zh) * | 2015-06-17 | 2015-10-21 | 蚌埠市天星树脂有限责任公司 | 一种健脾益气保健药品原药提取所用树脂及其制备方法 |
CN109077325A (zh) * | 2018-04-24 | 2018-12-25 | 恩施德源健康科技发展有限公司 | 一种高效增强免疫力的富硒植物肽制品及其制备方法和应用 |
CN109010506A (zh) * | 2018-09-18 | 2018-12-18 | 右江民族医学院 | 一种增强免疫力的口服液及其制备方法 |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN1113778A (zh) * | 1994-06-17 | 1995-12-27 | 殷广全 | 老年病制剂 |
CN1310003A (zh) * | 2001-02-19 | 2001-08-29 | 孟庆云 | 中西医治疗肺气肿的药物 |
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CN1113778A (zh) * | 1994-06-17 | 1995-12-27 | 殷广全 | 老年病制剂 |
CN1310003A (zh) * | 2001-02-19 | 2001-08-29 | 孟庆云 | 中西医治疗肺气肿的药物 |
Non-Patent Citations (1)
Title |
---|
补中益气汤在提高机体免疫力方面的应用100例临床观察 何晓凤 等,现代中医药,第2期 2003 * |
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