CN1207553C - 总黄酮含量的测定方法 - Google Patents
总黄酮含量的测定方法 Download PDFInfo
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- CN1207553C CN1207553C CN 03114240 CN03114240A CN1207553C CN 1207553 C CN1207553 C CN 1207553C CN 03114240 CN03114240 CN 03114240 CN 03114240 A CN03114240 A CN 03114240A CN 1207553 C CN1207553 C CN 1207553C
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- measured
- flavone content
- liquid
- total flavone
- light absorption
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- 229930003944 flavone Natural products 0.000 title claims abstract description 50
- 235000011949 flavones Nutrition 0.000 title claims abstract description 50
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 43
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 42
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 17
- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 239000000284 extract Substances 0.000 claims abstract description 13
- 239000012086 standard solution Substances 0.000 claims abstract description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 48
- 230000031700 light absorption Effects 0.000 claims description 32
- 235000001759 Citrus maxima Nutrition 0.000 claims description 30
- 244000276331 Citrus maxima Species 0.000 claims description 30
- 238000003556 assay Methods 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000013558 reference substance Substances 0.000 abstract description 11
- 238000003908 quality control method Methods 0.000 abstract description 6
- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 abstract description 4
- 229930019673 naringin Natural products 0.000 abstract description 4
- 229940052490 naringin Drugs 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000005070 sampling Methods 0.000 abstract description 2
- 240000000560 Citrus x paradisi Species 0.000 abstract 6
- 238000002835 absorbance Methods 0.000 abstract 4
- 239000004615 ingredient Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 11
- 150000002213 flavones Chemical class 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 5
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 5
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 5
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 5
- 235000005493 rutin Nutrition 0.000 description 5
- 229960004555 rutoside Drugs 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000012452 mother liquor Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000012925 reference material Substances 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- QUQPHWDTPGMPEX-UHFFFAOYSA-N Hesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(COC4C(C(O)C(O)C(C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-UHFFFAOYSA-N 0.000 description 1
- RPMNUQRUHXIGHK-PYXJVEIZSA-N apigenin 7-O-neohesperidoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C=C(C=3C=CC(O)=CC=3)OC2=C1 RPMNUQRUHXIGHK-PYXJVEIZSA-N 0.000 description 1
- 229930034861 apigenin-7-O-neohesperidoside Natural products 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- QUQPHWDTPGMPEX-QJBIFVCTSA-N hesperidin Chemical compound C1=C(O)C(OC)=CC=C1[C@H]1OC2=CC(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]4[C@@H]([C@H](O)[C@@H](O)[C@H](C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-QJBIFVCTSA-N 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- RPMNUQRUHXIGHK-SBDOOABHSA-N rhoifolin Natural products O([C@@H]1[C@@H](O)[C@H](O)[C@H](CO)O[C@H]1Oc1cc(O)c2C(=O)C=C(c3ccc(O)cc3)Oc2c1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](C)O1 RPMNUQRUHXIGHK-SBDOOABHSA-N 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
Images
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
方 法 | 对照品 | 检测波长(nm) | 柚皮苷含量(%) | 总黄酮含量(%) |
HPLC | 柚皮苷 | 283 | 10 | - |
芦丁对照法 | 芦丁 | 360 | - | 1 |
Al3+络合法 | 柚皮苷 | 351 | - | 7 |
本发明 | 柚皮苷 | 283 | - | 11 |
检测日期 | 总黄酮含量(%) | RSD(%) |
2002.4.11 | 10.95 | 0.23 |
2002.6.24 | 10.94 | |
2002.7.8 | 10.98 | |
2003.1.6 | 11.01 |
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 03114240 CN1207553C (zh) | 2003-04-14 | 2003-04-14 | 总黄酮含量的测定方法 |
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CN 03114240 CN1207553C (zh) | 2003-04-14 | 2003-04-14 | 总黄酮含量的测定方法 |
Publications (2)
Publication Number | Publication Date |
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CN1442687A CN1442687A (zh) | 2003-09-17 |
CN1207553C true CN1207553C (zh) | 2005-06-22 |
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CN 03114240 Expired - Lifetime CN1207553C (zh) | 2003-04-14 | 2003-04-14 | 总黄酮含量的测定方法 |
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Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102230892B (zh) * | 2011-06-21 | 2013-04-17 | 新疆全安药业有限公司 | 一种检测安胃疡中总黄酮含量的方法 |
CN102426156A (zh) * | 2011-11-22 | 2012-04-25 | 江苏大学 | 松花粉超临界萃取物中总黄酮含量的测定方法 |
CN103822888B (zh) * | 2013-11-21 | 2016-05-25 | 泸州医学院 | 赶黄草的质量检测方法 |
CN105259124A (zh) * | 2015-10-20 | 2016-01-20 | 河北英都气化有限公司 | 一种检测一氧化碳气体中的五羰基铁浓度的方法 |
CN106018297A (zh) * | 2016-05-20 | 2016-10-12 | 大连大学 | 蓝莓中总黄酚含量的测定 |
CN106018296A (zh) * | 2016-05-20 | 2016-10-12 | 大连大学 | 蓝莓中总黄酮含量的测定 |
CN106769930A (zh) * | 2016-12-16 | 2017-05-31 | 四川农业大学 | 盐、干旱和uv‑b胁迫下苦荞总黄酮含量的测定方法 |
CN107179286A (zh) * | 2017-06-29 | 2017-09-19 | 远大医药(中国)有限公司 | 一种测定吲达帕胺含量的方法 |
CN110542664A (zh) * | 2019-09-09 | 2019-12-06 | 湖北省农业科学院中药材研究所 | 一种检测藤茶中总黄酮类化合物含量的方法 |
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2003
- 2003-04-14 CN CN 03114240 patent/CN1207553C/zh not_active Expired - Lifetime
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Owner name: MEIYAN, MEI COUNTY, GUAN DONG CYANOBACTERIA CO., Free format text: FORMER OWNER: MEIYAN INST OF HI-TECH SPIRULINA, MEIZHOU CITY, GUANGDONG Effective date: 20051118 |
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Assignee: Honz Pharmaceutical Co.,Ltd. Assignor: GUANGDONG MEIXIAN MEIYAN CYANOBACTERIA CO.,LTD. Contract fulfillment period: 2008.9.9 to 2013.9.9 Contract record no.: 2008460000009 Denomination of invention: Method for measuring general flavone content in mangrove bruguiera Granted publication date: 20050622 License type: Exclusive license Record date: 20081104 |
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Effective date of registration: 20090123 Address after: No. 168, Haikou Free Trade Zone, Haikou Nanhai Avenue, Hainan, China Patentee after: Honz Pharmaceutical Co.,Ltd. Address before: Wild Goose Town, Guangdong, Meixian Patentee before: GUANGDONG MEIXIAN MEIYAN CYANOBACTERIA CO.,LTD. |
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