CN1207553C - Determination method of total flavone content - Google Patents
Determination method of total flavone content Download PDFInfo
- Publication number
- CN1207553C CN1207553C CN 03114240 CN03114240A CN1207553C CN 1207553 C CN1207553 C CN 1207553C CN 03114240 CN03114240 CN 03114240 CN 03114240 A CN03114240 A CN 03114240A CN 1207553 C CN1207553 C CN 1207553C
- Authority
- CN
- China
- Prior art keywords
- measured
- flavone content
- liquid
- total flavone
- light absorption
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229930003944 flavone Natural products 0.000 title claims abstract description 50
- 235000011949 flavones Nutrition 0.000 title claims abstract description 50
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 43
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 42
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 17
- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 239000000284 extract Substances 0.000 claims abstract description 13
- 239000012086 standard solution Substances 0.000 claims abstract description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 48
- 230000031700 light absorption Effects 0.000 claims description 32
- 235000001759 Citrus maxima Nutrition 0.000 claims description 30
- 244000276331 Citrus maxima Species 0.000 claims description 30
- 238000003556 assay Methods 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000013558 reference substance Substances 0.000 abstract description 11
- 238000003908 quality control method Methods 0.000 abstract description 6
- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 abstract description 4
- 229930019673 naringin Natural products 0.000 abstract description 4
- 229940052490 naringin Drugs 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000005070 sampling Methods 0.000 abstract description 2
- 240000000560 Citrus x paradisi Species 0.000 abstract 6
- 238000002835 absorbance Methods 0.000 abstract 4
- 239000004615 ingredient Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 11
- 150000002213 flavones Chemical class 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 5
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 5
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 5
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 5
- 235000005493 rutin Nutrition 0.000 description 5
- 229960004555 rutoside Drugs 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000012452 mother liquor Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000012925 reference material Substances 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- QUQPHWDTPGMPEX-UHFFFAOYSA-N Hesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(COC4C(C(O)C(O)C(C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-UHFFFAOYSA-N 0.000 description 1
- RPMNUQRUHXIGHK-PYXJVEIZSA-N apigenin 7-O-neohesperidoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C=C(C=3C=CC(O)=CC=3)OC2=C1 RPMNUQRUHXIGHK-PYXJVEIZSA-N 0.000 description 1
- 229930034861 apigenin-7-O-neohesperidoside Natural products 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- QUQPHWDTPGMPEX-QJBIFVCTSA-N hesperidin Chemical compound C1=C(O)C(OC)=CC=C1[C@H]1OC2=CC(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]4[C@@H]([C@H](O)[C@@H](O)[C@H](C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-QJBIFVCTSA-N 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- RPMNUQRUHXIGHK-SBDOOABHSA-N rhoifolin Natural products O([C@@H]1[C@@H](O)[C@H](O)[C@H](CO)O[C@H]1Oc1cc(O)c2C(=O)C=C(c3ccc(O)cc3)Oc2c1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](C)O1 RPMNUQRUHXIGHK-SBDOOABHSA-N 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The present invention relates to a method for measuring the total flavone content in grapefruits or substances with the same ingredients with the total flavone ingredients of grapefruits. In the method, dried grapefruits or extracts thereof are dissolved to be prepared into liquid to be measured, naringin with the standard fineness of more than 99.9% is used as a reference substance to prepare a gradient standard solution, under the wave length of 283 nm, the absorbance values of the standard solution are respectively measured, and a standard curve is established according to the corresponding relation of the absorbance values and concentration; the absorbance values of the liquid to be measured are measured under the wave length of 283 nm, according to the obtained absorbance values, the concentration of the liquid to be measured is checked by the standard curve, the concentration value is the total flavone content of the liquid to be measured, and the total flavone content of grapefruits or extracts thereof is converted according to the extension rate. The present invention has the advantages of accurate detection result, strong pertinence, convenient operation, good stability, easy control and good repeatability. The present invention is applied to the quality control of raw materials, such as the total flavone content of grapefruits, the quality control of semi-products, such as the total flavone content in grapefruit extracts, and the quality control of finished products, such as the total flavone content sampling detection of exfactory products, by the applicant.
Description
Technical field
The present invention relates to a kind of method of measuring content of material, particularly a kind of method of measuring general flavone content in shaddock or the material identical with shaddock general flavone composition.
Background technology
Flavones is the big class material that occurring in nature extensively exists, a lot of contained flavones of plant do not exist with single composition, and exist jointly with the close or similar flavones of several structures, as shaddock, its contained flavones is mainly aurantiin, also contains other flavones such as a small amount of hesperidine, Rhoifolin.The method of measuring general flavone content is a lot, and does not specifically appear in the newspapers so far at the assay method of general flavone content in the shaddock.Many in the method for existing mensuration general flavone content is standard reference material with the rutin, with methyl alcohol is solvent, detect under the wavelength at 360nm, measure its light absorption value, have linear relationship between its light absorption value and the concentration, the production standard curve is according to this typical curve, the concentration of solution to be measured can be drawn from the light absorption value of solution to be measured, the determinand general flavone content can be conversed according to this concentration value.But owing to be standard reference material with the rutin, it detects wavelength is 360nm, and the 360nm place, the light absorption value of aurantiin is very little, and by the UV scanning figure of aurantiin as can be known, the ultraviolet maximum absorption band of aurantiin is 283 ± 1nm, at the 360nm place, therefore the light absorption value of aurantiin adopts this method to measure general flavone content in the shaddock near trough, and its measurement result is seriously less than normal.In addition, also have a kind of method that is usually used in detecting general flavone content, that is dissolved in methyl alcohol with testing sample exactly, adds AlCl under alkali condition
3Solution allows in the flavones
Structure and Al
3+Complexing, the standard items with testing sample are contrast then, according to the ultimate principle of ultraviolet spectrophotometry, serve as to detect wavelength, production standard curve with the wavelength at ultraviolet region maximum absorption band place.In the same way determinand is carried out pre-service, measure liquid light absorption value to be measured, can try to achieve liquid concentration to be measured by typical curve, converse the determinand general flavone content at last, though this method is widely used, but be difficult to control when being used for measuring the shaddock general flavone content, because in testing process, its light absorption value is constantly diminishing, test according to time-coursre, when this method was used for detecting shaddock or its extract general flavone content, less stable can't be used for shaddock or its extract determination of total flavonoids.
Summary of the invention
The purpose of invention just provides the method for general flavone content in a kind of accurate, convenient mensuration shaddock or the material identical with shaddock general flavone composition.
The present invention can be realized by following mode: (1) is earlier with dried shaddock or the dissolving of its extract, be mixed with liquid to be measured, compare product with standard purity 99.9% above aurantiin then, be mixed with the standard solution of gradient, under the 283nm wavelength, measure the light absorption value of this standard solution respectively, set up typical curve according to the corresponding relation between light absorption value and the concentration; (2) liquid to be measured is surveyed light absorption value under the 283nm wavelength, according to the gained light absorption value, check in this liquid concentration to be measured by typical curve, this concentration value is liquid general flavone content to be measured, converses shaddock or its extractive total flavone content according to extension rate then.Dissolving shaddock and extract and the used solvent of preparation aurantiin standard solution are methyl alcohol or absolute ethyl alcohol.
The present invention has the following advantages:
1, testing result is accurate, with strong points.
The present invention is specially at the mensuration of general flavone content in shaddock or its extract, the absorption at 283 ± 1nm wavelength place, for
Structure is at the absorption maximum place of ultraviolet, and contained flavones all has this structure in the shaddock, so the light absorption value at 283nm place, is total light absorption value of multiple flavones, and measurement result is more accurate.With shaddock powder (lot number is 2002-04-09) is example, utilizing HPLC method (Pharmacopoeia of People's Republic of China one one＜2000 editions〉Exocarpium Citri Grandis item under provide) to detect naringin content is 10%, with the rutin is reference substance, 360nm is for detecting wavelength, recording its general flavone content is about 1%, with the aurantiin is reference substance, Al
3+It is 7% that complexometry records general flavone content, and it is 11% that the present invention records general flavone content, and measurement result sees Table 1.As seen the present invention has more plateau confidence level than similar assay method.In addition, show through the application of sample recovery test that this method average recovery can reach 99%, measurement result is reliable.
Table 1: lot number is that flavones content is measured relatively in the 2002-04-09 shaddock
| Method | Reference substance | Detect wavelength (nm) | Naringin content (%) | General flavone content (%) |
| HPLC | Aurantiin | 283 | 10 | - |
| The rutin counter point | Rutin | 360 | - | 1 |
| Al 3+Complexometry | Aurantiin | 351 | - | 7 |
| The present invention | Aurantiin | 283 | - | 11 |
2, easy and simple to handle
Use step of the present invention few, need not add alkali and complexometric reagent, after the testing sample pre-service, can utilize ultraviolet spectrophotometer to detect, easy to operate.
3, good stability is easy to control
The present invention shows through time-coursre test, and no change in its light absorption value 48 hours is seen Fig. 2, Fig. 3.And employing Al
3+When complexometry is measured shaddock and extract thereof, stable extreme difference, its light absorption value constantly diminished in 24 hours.
4, good reproducibility
The present invention is for same sample, and each testing result relative deviation is no more than 0.5%, sees Table 2.
Table 2: lot number is four measurement result comparison sheets of 2002-04-09 shaddock powder general flavone content
| Detect the date | General flavone content (%) | RSD(％) |
| 2002.4.11 | 10.95 | 0.23 |
| 2002.6.24 | 10.94 | |
| 2002.7.8 | 10.98 | |
| 2003.1.6 | 11.01 |
The positive defending party to the application of the present invention uses in raw material quality control, as general flavone content in the shaddock; Semi-manufactured goods quality control is as general flavone content in the shaddock extract; And end product quality control, as the product sampling Detection general flavone content that dispatches from the factory.
Description of drawings
Below in conjunction with accompanying drawing, the invention will be further described.
Fig. 1 is aurantiin UV scanning figure of the present invention.
Fig. 2 is aurantiin reference substance TIME COURSE of the present invention figure as a result, A be the 1d light absorption value over time.
Fig. 3 is aurantiin reference substance TIME COURSE of the present invention figure as a result, B be the 2d light absorption value over time.
Fig. 4 is a typical curve of the present invention.
Embodiment
Concrete operations step of the present invention is as follows:
1, will analyze pure 99.9% above aurantiin reference substance and be heated to constant weight for 110 ℃;
2, accurately take by weighing this reference substance 0.005g, be dissolved in and analyze pure methyl alcohol, and with methanol constant volume to 25ml, as mother liquor;
3, from the good volumetric flask of constant volume, draw in naringin mother liquor 0ml, 0.5ml, 1ml, 2ml, 4ml, 8ml to the 25ml volumetric flask respectively, distinguish constant volume, then obtain the aurantiin standard solution of 0 μ g/ml, 4 μ g/ml, 8 μ g/ml, 16 μ g/ml, 32 μ g/ml, 64 μ g/ml;
4, with methyl alcohol being blank, serves as to detect the wavelength difference light absorption value of above standard solution in order with 283 ± 1nm;
5, being ordinate with the light absorption value, is horizontal ordinate with concentration, sets up the corresponding relation between light absorption value and the concentration, the production standard curve;
6, determinand is dried to constant weight for 110 ℃, accurately take by weighing a certain amount of determinand, dissolving methyl alcohol, fixed molten to certain volume, carry out bigness scale earlier, if its light absorption value is outside the range of linearity, survey again after then should diluting, make its light absorption value within standard curve range, the light absorption value that obtains draws corresponding concentration by typical curve, multiply by extension rate again and promptly gets general flavone content in the determinand.
Its computing formula is:
General flavone content in the A-determinand
C-checks in solution concentration to be measured (μ g/ml) according to typical curve by the determinand light absorption value
V-dissolving and the dilution used methyl alcohol volume of determinand (ml)
M-determinand quality (μ g)
Determination of total flavonoids in example one shaddock
1, shaddock is cleaned, be crushed to meal, drying, randomly draw certain sample.
2, testing sample is dried to constant weight for 110 ℃, accurately takes by weighing the 1g sample and put in the Soxhlet extractor, add boiling range 60-90 ℃ of sherwood oil 80ml, reflux 2-3 hour, discard sherwood oil, the shaddock slag is flung to sherwood oil.
3, add methyl alcohol 80ml, reflux was put cold filtration to extracting liquid colourless in 3 hours, and filtrate is put in the 100ml dissolve measuring bottle, uses the small amount of methanol washing container again, and washing lotion is filtered in the same volumetric flask, adds methyl alcohol to scale, shakes up.
4, accurately draw this solution of 1ml and put in the 25ml volumetric flask, add methyl alcohol to scale, shake up and be liquid to be measured.
5, with the aurantiin reference substance, provided by Nat'l Pharmaceutical ﹠ Biological Products Control Institute, 110 ℃ are dried to constant weight, take by weighing aurantiin reference substance 0.003g, put in the 25ml volumetric flask, add dissolve with methanol, and constant volume, shake up, as mother liquor.
6, accurately draw reference substance solution 0ml, 0.5ml, 1.0ml, 2.0ml, 4.0ml, 8.0ml, 10.0ml respectively in the 25ml volumetric flask, constant volume is to scale respectively, shake up, then its concentration is respectively 0 μ g/ml, 2.4 μ g/ml, 4.8 μ g/ml, 9.6 μ g/ml, 19.2 μ g/ml, 38.4 μ g/ml, 48.0 μ g/ml.
7, surveying light absorption value respectively under the 283nm wavelength is: 0,0.082,0.165,0.326,0.657,1.313,1.637.
8, with the light absorption value be ordinate, concentration is horizontal ordinate, sets up typical curve, sees Fig. 4.
9, liquid to be measured is put under the 283nm wavelength and to be surveyed light absorption value and be: 1.601, getting liquid concentration to be measured by typical curve is 44ug/ml.
10, by computing formula
Try to achieve that general flavone content is 11% in the shaddock dry powder.
Example two shaddock extractive total flavone assays
1, the shaddock extract is dried to constant weight for 110 ℃, accurately takes by weighing this sample 0.005g, put in the 25ml volumetric flask and use dissolve with methanol, and constant volume, shake up.
2, get in 1ml to the 25ml volumetric flask, add methyl alcohol, get liquid to be measured, if testing sample can not dissolve desirable clarified solution fully to scale.
3, standard curve making is with example one.
4, liquid to be measured being surveyed light absorption value down in the 283nm wavelength is 0.262, and can get its concentration by typical curve is 7.6 μ g/ml, by computing formula
Try to achieve this extractive total flavone and contain 95%.
Claims (2)
1, a kind of assay method of general flavone content is characterized in that:
(1) earlier with dried shaddock or the dissolving of its extract, be mixed with liquid to be measured, compare product with standard purity 99.9% above aurantiin then, be mixed with the standard solution of gradient, under the 283nm wavelength, measure the light absorption value of this standard solution respectively, set up typical curve according to the corresponding relation between light absorption value and the concentration;
(2) liquid to be measured is surveyed light absorption value under the 283nm wavelength, according to the gained light absorption value, check in this liquid concentration to be measured by typical curve, this concentration value is liquid general flavone content to be measured, converses shaddock or its extractive total flavone content according to extension rate then.
2, according to the described method of claim 1, it is characterized in that dissolving shaddock and extract and preparing the used solvent of aurantiin standard solution is methyl alcohol or absolute ethyl alcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03114240 CN1207553C (en) | 2003-04-14 | 2003-04-14 | Determination method of total flavone content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03114240 CN1207553C (en) | 2003-04-14 | 2003-04-14 | Determination method of total flavone content |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1442687A CN1442687A (en) | 2003-09-17 |
| CN1207553C true CN1207553C (en) | 2005-06-22 |
Family
ID=27797018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03114240 Expired - Lifetime CN1207553C (en) | 2003-04-14 | 2003-04-14 | Determination method of total flavone content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1207553C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102230892B (en) * | 2011-06-21 | 2013-04-17 | 新疆全安药业有限公司 | Method for detecting general flavone content of gastric ulcer medicament |
| CN102426156A (en) * | 2011-11-22 | 2012-04-25 | 江苏大学 | Determination method for total flavone content in pollen pini supercritical extractant |
| CN103822888B (en) * | 2013-11-21 | 2016-05-25 | 泸州医学院 | Catch up with the quality determining method of yellow grass |
| CN105259124A (en) * | 2015-10-20 | 2016-01-20 | 河北英都气化有限公司 | Method for detecting concentration of pentacarbonyl iron in carbon monoxide gas |
| CN106018297A (en) * | 2016-05-20 | 2016-10-12 | 大连大学 | Method for measuring content of total phenols in blueberries |
| CN106018296A (en) * | 2016-05-20 | 2016-10-12 | 大连大学 | Method for measuring content of total flavonoids in blueberries |
| CN106769930A (en) * | 2016-12-16 | 2017-05-31 | 四川农业大学 | The assay method of the lower bitter buckwheat general flavone content of salt, arid and UV B stress |
| CN107179286A (en) * | 2017-06-29 | 2017-09-19 | 远大医药（中国）有限公司 | A kind of method for determining indapamide content |
| CN110542664A (en) * | 2019-09-09 | 2019-12-06 | 湖北省农业科学院中药材研究所 | Method for detecting content of total flavonoids in vine tea |
-
2003
- 2003-04-14 CN CN 03114240 patent/CN1207553C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1442687A (en) | 2003-09-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102183517B (en) | Method for determining general flavone content | |
| CN1207553C (en) | Determination method of total flavone content | |
| CN103558214B (en) | Lack the assay method of polyphenol content in luxuriant arrow bamboo | |
| CN110015992A (en) | Fluorescence probe and its preparation method and application of the one kind for the detection of sulfur dioxide/sulfurous acid (hydrogen) salt | |
| CN106248607A (en) | Utilize the method that first derivative ultraviolet spectro-photometry measures oligochitosan deacetylation | |
| CN100578216C (en) | Quantitative determination method for fluorescent whitening agent VBL, ABP for tobacco | |
| CN108613952A (en) | A kind of analysis determining method of myricetin | |
| Kulić et al. | A detailed view on the proanthocyanidins in Ginkgo extract EGb 761 | |
| Basavaiah et al. | Spectrophotometric determination of ceterizine hydrochloride with Alizarin Red S | |
| CN102507466B (en) | Improved spectrophotometry method for determining proteins by using Coomassie brilliant blue | |
| CN106290203B (en) | A kind of tetracycline colorimetric detection method based on Catalysis by Hemin reaction | |
| CN103822888A (en) | Quality test method for penthorum Chinense pursh | |
| WO2009155755A1 (en) | Method for determining the contents of oligosaccharides in morinda officinalis | |
| CN103543222A (en) | Reduning injection saccharide content detection method | |
| Erk | Extractive spectrophotometric methods for determination of lercanidipine | |
| CN104198418A (en) | High-flux determination method for aureomycin titer | |
| CN1261749C (en) | Method for rapidly and quantitatively determining the triterpenoid content in ganoderma lucidum | |
| CN102243215A (en) | Detection method for water-soluble glucomannan | |
| CN113009023A (en) | Method for measuring chemical component content in traditional Chinese medicine compound preparation | |
| CN104655602B (en) | A kind of hydrophobic ionic liquid [MBPy] Tf2The fluorescence detection method of N | |
| CN1289908C (en) | Determining method for Chinese yew plant and taxol in its cell culture and homolog | |
| CN101639460A (en) | Method for measuring general flavone content in mangrove bruguiera | |
| CN101303334B (en) | HPLC testing method of Nosiheptide product biological valence content | |
| Antakli et al. | Determination of Amoxicillin Trihydrate by Analytical Spectrophotometry | |
| CN108051379A (en) | A kind of content assaying method of Cimetidine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: MEIYAN, MEI COUNTY, GUAN DONG CYANOBACTERIA CO., Free format text: FORMER OWNER: MEIYAN INST OF HI-TECH SPIRULINA, MEIZHOU CITY, GUANGDONG Effective date: 20051118 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20051118 Address after: 514759, Meixian, Guangdong province goose Yang Town Patentee after: GUANGDONG MEIXIAN MEIYAN CYANOBACTERIA CO.,LTD. Address before: 514700 Li Jinlian, Yan Yang Town, Guangdong, Meixian Patentee before: MEIYAN INST OF SPIRULINA HIGH |
|
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Honz Pharmaceutical Co.,Ltd. Assignor: GUANGDONG MEIXIAN MEIYAN CYANOBACTERIA CO.,LTD. Contract fulfillment period: 2008.9.9 to 2013.9.9 Contract record no.: 2008460000009 Denomination of invention: Method for measuring general flavone content in mangrove bruguiera Granted publication date: 20050622 License type: Exclusive license Record date: 20081104 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20090123 Address after: No. 168, Haikou Free Trade Zone, Haikou Nanhai Avenue, Hainan, China Patentee after: Honz Pharmaceutical Co.,Ltd. Address before: Wild Goose Town, Guangdong, Meixian Patentee before: GUANGDONG MEIXIAN MEIYAN CYANOBACTERIA CO.,LTD. |
|
| ASS | Succession or assignment of patent right |
Owner name: HAINAN KANGZHI PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: GUANGDONG MEI COUNTY MEIYAN CYANOBACTERIA CO., LTD. Effective date: 20090123 |
|
| LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.9.9 TO 2013.9.9; CHANGE OF CONTRACT Name of requester: HAINAN KANGZHI PHARMACEUTICAL CO., LTD. Effective date: 20081104 |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 570216 Hainan South Road, Haikou, No. 168, Haikou Free Trade Zone No. Patentee after: HAINAN HONZ PHARMACEUTICAL Co.,Ltd. Address before: 570216 Hainan South Road, Haikou, No. 168, Haikou Free Trade Zone No. Patentee before: Honz Pharmaceutical Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder | ||
| DD01 | Delivery of document by public notice |
Addressee: Lu Fangmei Document name: Notification to Pay the Fees |
|
| DD01 | Delivery of document by public notice | ||
| CX01 | Expiry of patent term |
Granted publication date: 20050622 |
|
| CX01 | Expiry of patent term | ||
| DD01 | Delivery of document by public notice |
Addressee: Lu Fangmei Document name: Notice of Termination of Patent Rights |
|
| DD01 | Delivery of document by public notice |