CN1190993A - 基因表达的启动子 - Google Patents
基因表达的启动子 Download PDFInfo
- Publication number
- CN1190993A CN1190993A CN96195518A CN96195518A CN1190993A CN 1190993 A CN1190993 A CN 1190993A CN 96195518 A CN96195518 A CN 96195518A CN 96195518 A CN96195518 A CN 96195518A CN 1190993 A CN1190993 A CN 1190993A
- Authority
- CN
- China
- Prior art keywords
- recombinant dna
- dna constructs
- promotor
- sequence
- host
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/635—Parathyroid hormone (parathormone); Parathyroid hormone-related peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Abstract
Description
启动子 | 核蛋白体结合位点 | Max PTH(mg/L)6-8小时 | |||
# | -35区 | 间隔区 | -10区 | ||
1 | trp | lpp | lppP-5 | T5lac | 245148 |
2 | trp | lacUV5 | lppP-5 | T5lac | 12110 |
3 | trp | lacO | lppP-5 | T5lac | 505 |
4 | T7 | lacO | T7 | T5lac | 10010 |
Claims (11)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/445,133 US5629205A (en) | 1995-05-19 | 1995-05-19 | Promoters for gene expression |
US08/445,133 | 1995-05-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1190993A true CN1190993A (zh) | 1998-08-19 |
CN1131313C CN1131313C (zh) | 2003-12-17 |
Family
ID=23767732
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN96195518A Expired - Lifetime CN1131313C (zh) | 1995-05-19 | 1996-05-16 | 基因表达的启动子 |
Country Status (25)
Country | Link |
---|---|
US (1) | US5629205A (zh) |
EP (1) | EP0828844B1 (zh) |
JP (2) | JP3828576B2 (zh) |
KR (1) | KR19990014906A (zh) |
CN (1) | CN1131313C (zh) |
AT (1) | ATE248923T1 (zh) |
AU (1) | AU698656B2 (zh) |
BR (1) | BR9609102A (zh) |
CA (1) | CA2221587C (zh) |
CZ (1) | CZ357997A3 (zh) |
DE (1) | DE69629808T2 (zh) |
DK (1) | DK0828844T3 (zh) |
EE (1) | EE9700355A (zh) |
ES (1) | ES2202436T3 (zh) |
HK (1) | HK1004148A1 (zh) |
HU (1) | HUP9900750A3 (zh) |
IS (1) | IS4609A (zh) |
MX (1) | MX9708689A (zh) |
NO (1) | NO325879B1 (zh) |
NZ (1) | NZ306536A (zh) |
PL (1) | PL324226A1 (zh) |
PT (1) | PT828844E (zh) |
SK (1) | SK154997A3 (zh) |
TR (1) | TR199701394T1 (zh) |
WO (1) | WO1996036721A1 (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108060168A (zh) * | 2017-12-29 | 2018-05-22 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的t载体和应用 |
CN108118059A (zh) * | 2017-12-30 | 2018-06-05 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的载体和应用 |
CN108165551A (zh) * | 2017-12-30 | 2018-06-15 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的t载体和应用 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6194168B1 (en) | 1997-09-30 | 2001-02-27 | Human Genome Sciences, Inc. | Expression control sequences |
EP1572720A4 (en) * | 2002-05-24 | 2008-12-24 | Nps Allelix Corp | PROCESS FOR THE ENZYMATIC PRODUCTION OF GLP-2 (1-34) AND GLP-2 (1-33) PEPTIDES |
US20050119183A1 (en) * | 2003-11-12 | 2005-06-02 | Nps Allelix Corp. | Method for treating bone loss using parathyroid hormone |
DK1704234T3 (da) * | 2003-11-21 | 2012-05-07 | Nps Pharma Inc | Fremstilling af glucagon-lignende peptid 2 og analoger |
WO2005058946A2 (en) * | 2003-12-12 | 2005-06-30 | Zymogenetics, Inc. | Methods for enhancing expression of recombinant proteins |
MA45489A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
KR102606210B1 (ko) | 2017-04-27 | 2023-11-24 | 주노 테라퓨틱스 게엠베하 | 올리고머 입자 시약 및 이의 사용 방법 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8517071D0 (en) * | 1985-07-05 | 1985-08-14 | Hoffmann La Roche | Gram-positive expression control sequence |
ATE123526T1 (de) * | 1987-08-17 | 1995-06-15 | Hoffmann La Roche | Hochreprimierbare expressionskontrollsequenzen. |
US5223407A (en) * | 1988-08-31 | 1993-06-29 | Allelix Inc. | Excretion of heterologous proteins from e. coli |
US5171670A (en) * | 1989-05-12 | 1992-12-15 | The General Hospital Corporation | Recombinant dna method for production of parathyroid hormone |
JPH05509001A (ja) * | 1990-08-28 | 1993-12-16 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | 有効な分泌用ベクター類の迅速選択方法 |
-
1995
- 1995-05-19 US US08/445,133 patent/US5629205A/en not_active Expired - Lifetime
-
1996
- 1996-05-16 EP EP96912181A patent/EP0828844B1/en not_active Expired - Lifetime
- 1996-05-16 DE DE69629808T patent/DE69629808T2/de not_active Expired - Lifetime
- 1996-05-16 CA CA002221587A patent/CA2221587C/en not_active Expired - Lifetime
- 1996-05-16 SK SK1549-97A patent/SK154997A3/sk unknown
- 1996-05-16 PT PT96912181T patent/PT828844E/pt unknown
- 1996-05-16 HU HU9900750A patent/HUP9900750A3/hu unknown
- 1996-05-16 AU AU55116/96A patent/AU698656B2/en not_active Expired
- 1996-05-16 TR TR97/01394T patent/TR199701394T1/xx unknown
- 1996-05-16 ES ES96912181T patent/ES2202436T3/es not_active Expired - Lifetime
- 1996-05-16 CN CN96195518A patent/CN1131313C/zh not_active Expired - Lifetime
- 1996-05-16 PL PL96324226A patent/PL324226A1/xx unknown
- 1996-05-16 KR KR1019970708251A patent/KR19990014906A/ko not_active Application Discontinuation
- 1996-05-16 NZ NZ306536A patent/NZ306536A/en not_active IP Right Cessation
- 1996-05-16 BR BR9609102A patent/BR9609102A/pt unknown
- 1996-05-16 WO PCT/IB1996/000462 patent/WO1996036721A1/en active IP Right Grant
- 1996-05-16 MX MX9708689A patent/MX9708689A/es unknown
- 1996-05-16 CZ CZ973579A patent/CZ357997A3/cs unknown
- 1996-05-16 JP JP53467596A patent/JP3828576B2/ja not_active Expired - Lifetime
- 1996-05-16 AT AT96912181T patent/ATE248923T1/de active
- 1996-05-16 EE EE9700355A patent/EE9700355A/xx unknown
- 1996-05-16 DK DK96912181T patent/DK0828844T3/da active
-
1997
- 1997-11-07 IS IS4609A patent/IS4609A/is unknown
- 1997-11-18 NO NO19975286A patent/NO325879B1/no not_active IP Right Cessation
-
1998
- 1998-04-24 HK HK98103447A patent/HK1004148A1/xx not_active IP Right Cessation
-
2006
- 2006-03-13 JP JP2006066844A patent/JP3837154B2/ja not_active Expired - Lifetime
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108060168A (zh) * | 2017-12-29 | 2018-05-22 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的t载体和应用 |
CN108060168B (zh) * | 2017-12-29 | 2021-06-29 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的t载体和应用 |
CN108118059A (zh) * | 2017-12-30 | 2018-06-05 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的载体和应用 |
CN108165551A (zh) * | 2017-12-30 | 2018-06-15 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的t载体和应用 |
CN108118059B (zh) * | 2017-12-30 | 2021-03-19 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的载体和应用 |
CN108165551B (zh) * | 2017-12-30 | 2021-06-29 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的t载体和应用 |
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C53 | Correction of patent of invention or patent application | ||
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Free format text: CORRECT: APPLICANT; FROM: AKTIEBOLAGET ASTRA TO: ALERICS NEUROSCIENCE CO |
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Address after: Ontario, Canada Applicant after: Alielikesi biological pharmaceutical company Address before: Swedish Sodertalje Applicant before: Astra Aktiebolag |
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Effective date of registration: 20090508 Address after: American Utah Patentee after: NPS Pharmaceuticals, Inc. Address before: Ontario, Canada Patentee before: NPS Alirix Inc. |
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Granted publication date: 20031217 |
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