CN118103494A - 具有生产n-乙酰神经氨酸和/或含n-乙酰神经氨酸的糖质的能力的微生物以及使用该微生物的n-乙酰神经氨酸和/或含n-乙酰神经氨酸的糖质的制造方法 - Google Patents

具有生产n-乙酰神经氨酸和/或含n-乙酰神经氨酸的糖质的能力的微生物以及使用该微生物的n-乙酰神经氨酸和/或含n-乙酰神经氨酸的糖质的制造方法 Download PDF

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Publication number
CN118103494A
CN118103494A CN202280067203.5A CN202280067203A CN118103494A CN 118103494 A CN118103494 A CN 118103494A CN 202280067203 A CN202280067203 A CN 202280067203A CN 118103494 A CN118103494 A CN 118103494A
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neuac
microorganism
strain
dna
activity
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Chinese (zh)
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直江大树
氏原哲朗
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Kyowa Hakko Bio Co Ltd
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Kyowa Hakko Bio Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01336—UDP-N-acetyl-D-mannosamine dehydrogenase (1.1.1.336)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)
    • C12Y207/08033—UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase (2.7.8.33)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03014—UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing) (5.1.3.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pulmonology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202280067203.5A 2021-10-08 2022-10-07 具有生产n-乙酰神经氨酸和/或含n-乙酰神经氨酸的糖质的能力的微生物以及使用该微生物的n-乙酰神经氨酸和/或含n-乙酰神经氨酸的糖质的制造方法 Pending CN118103494A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021166476 2021-10-08
JP2021-166476 2021-10-08
PCT/JP2022/037736 WO2023058772A1 (ja) 2021-10-08 2022-10-07 Ｎ－アセチルノイラミン酸及び／又はｎ－アセチルノイラミン酸含有糖質の生産能を有する微生物及び該微生物を用いたｎ－アセチルノイラミン酸及び／又はｎ－アセチルノイラミン酸含有糖質の製造方法

Publications (1)

Publication Number Publication Date
CN118103494A true CN118103494A (zh) 2024-05-28

Family

ID=85804393

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280067203.5A Pending CN118103494A (zh) 2021-10-08 2022-10-07 具有生产n-乙酰神经氨酸和/或含n-乙酰神经氨酸的糖质的能力的微生物以及使用该微生物的n-乙酰神经氨酸和/或含n-乙酰神经氨酸的糖质的制造方法

Country Status (6)

Country Link
US (1) US20240409974A1 (https=)
EP (1) EP4414447A4 (https=)
JP (1) JPWO2023058772A1 (https=)
CN (1) CN118103494A (https=)
AU (1) AU2022360817A1 (https=)
WO (1) WO2023058772A1 (https=)

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2545078B2 (ja) 1987-04-06 1996-10-16 協和醗酵工業株式会社 核酸関連物質の製造法
JP3131655B2 (ja) 1992-02-03 2001-02-05 マルキン忠勇株式会社 Ｎ−アセチルノイラミン酸の製造法
JP3944866B2 (ja) 1996-06-18 2007-07-18 マルキンバイオ株式会社 Ｎ−アセチルノイラミン酸シンターゼ、及びこれを用いるｎ−アセチルノイラミン酸の製造方法
CA2237849C (en) 1996-09-17 2009-06-16 Kyowa Hakko Kogyo Co., Ltd. Processes for producing sugar nucleotides and complex carbohydrates
JP3939096B2 (ja) 1999-02-09 2007-06-27 協和醗酵工業株式会社 Ｎ−アセチルグルコサミン２−エピメラーゼおよび該酵素をコードするｄｎａ
EP1447447B1 (en) 2001-09-26 2009-11-11 Kyowa Hakko Bio Co., Ltd. Process for producing sialic acid-containing complex sugar
US7329514B2 (en) 2002-02-28 2008-02-12 Kyowa Hakko Kogyo Co., Ltd. Process for producing n-acetylneuraminic acid
KR101035043B1 (ko) 2002-07-01 2011-05-19 아르키온 라이프 사이언씨즈 엘엘씨 글루코사민 및 ｎ-아세틸글루코사민의 제조를 위한 물질및 공정
JP5079272B2 (ja) 2006-06-30 2012-11-21 協和発酵バイオ株式会社 シチジン‐５´‐一リン酸‐ｎ‐アセチルノイラミン酸およびｎ‐アセチルノイラミン酸含有糖質の製造法
CN101809143A (zh) 2006-09-26 2010-08-18 锡拉丘兹大学 用于增加唾液酸产量的代谢工程大肠杆菌
US20110165626A1 (en) 2006-10-03 2011-07-07 Centre National De La Recherche Scientifique (Cnrs High yield production of sialic acid (neu5ac) by fermentation
JP2011067095A (ja) 2008-01-10 2011-04-07 Ajinomoto Co Inc 発酵法による目的物質の製造法
EP2675899A4 (en) 2011-02-16 2014-09-10 Glycosyn LLC BIOSYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES IN MANIPULATED BACTERIA
WO2015037698A1 (ja) 2013-09-12 2015-03-19 協和発酵バイオ株式会社 Ｎ－アセチルノイラミン酸及びｎ－アセチルノイラミン酸含有糖質の製造法
CA3171157A1 (en) * 2020-02-14 2021-08-19 Inbiose N.V. Production of glycosylated product in host cells
JP7483246B2 (ja) 2020-04-09 2024-05-15 株式会社スズテック 用土補給装置および用土補給方法

Also Published As

Publication number Publication date
EP4414447A4 (en) 2025-12-03
AU2022360817A1 (en) 2024-05-02
EP4414447A1 (en) 2024-08-14
WO2023058772A1 (ja) 2023-04-13
US20240409974A1 (en) 2024-12-12
JPWO2023058772A1 (https=) 2023-04-13

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