MX2009003212A - Escherichia coli metabolicamente desarrolla mediante ingenieria genetica para la produccion mejorada de acido sialico. - Google Patents

Escherichia coli metabolicamente desarrolla mediante ingenieria genetica para la produccion mejorada de acido sialico.

Info

Publication number
MX2009003212A
MX2009003212A MX2009003212A MX2009003212A MX2009003212A MX 2009003212 A MX2009003212 A MX 2009003212A MX 2009003212 A MX2009003212 A MX 2009003212A MX 2009003212 A MX2009003212 A MX 2009003212A MX 2009003212 A MX2009003212 A MX 2009003212A
Authority
MX
Mexico
Prior art keywords
sialic acid
coli
enchanced
production
escherichia coli
Prior art date
Application number
MX2009003212A
Other languages
English (en)
Inventor
Christopher N Boddy
Benjamin R Lundgren
Original Assignee
Univ Syracuse
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Syracuse filed Critical Univ Syracuse
Publication of MX2009003212A publication Critical patent/MX2009003212A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Una cepa de E. ccli metabólicamente desarrollada mediante ingeniería genética, la cual produce ácido siálico y un método para producir tal cepa. En las células de E. ccli desarrollada mediante ingeniería, los genes de nanT (transportador de ácido siálico) y nanA (adolasa de ácido siálico) están inactivos, y los genes de neuC y neuB de la biosíntesis del ácido siálico en el grupo de Neisseria meningitidis B se introducen y sobreexpresan en la célula de E. ccli nanT y nanA. Además, el gen de la glucosamina sintasa, glmS, de E. ccli se co-sobreexpresa con neuB y neuC.
MX2009003212A 2006-09-26 2007-09-26 Escherichia coli metabolicamente desarrolla mediante ingenieria genetica para la produccion mejorada de acido sialico. MX2009003212A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82691906P 2006-09-26 2006-09-26
PCT/US2007/079496 WO2008097366A2 (en) 2006-09-26 2007-09-26 Metabolically engineered escherichia coli for enchanced production of sialic acid

Publications (1)

Publication Number Publication Date
MX2009003212A true MX2009003212A (es) 2009-08-12

Family

ID=39682270

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009003212A MX2009003212A (es) 2006-09-26 2007-09-26 Escherichia coli metabolicamente desarrolla mediante ingenieria genetica para la produccion mejorada de acido sialico.

Country Status (10)

Country Link
US (2) US8722365B2 (es)
EP (1) EP2082029A2 (es)
JP (1) JP2010517509A (es)
KR (1) KR20090077782A (es)
CN (1) CN101809143A (es)
AU (1) AU2007346659A1 (es)
BR (1) BRPI0715253A2 (es)
MX (1) MX2009003212A (es)
RU (1) RU2009113700A (es)
WO (1) WO2008097366A2 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011130836A1 (en) * 2010-04-20 2011-10-27 National Research Council Of Canada Cell-based production of nonulosonates
CN102154163A (zh) * 2010-12-31 2011-08-17 朱莉 一种3′-唾液酸乳糖的制备方法
ES2700274T3 (es) 2013-03-14 2019-02-14 Glycosyn LLC Microorganismos y métodos para producir oligosacáridos sialilados y que contienen N-acetilglucosamina
CN103923869A (zh) * 2014-03-19 2014-07-16 武汉中科光谷绿色生物技术有限公司 一种产n-乙酰神经氨酸的枯草芽孢杆菌基因工程菌及其构建方法和应用
US10378034B2 (en) 2014-05-27 2019-08-13 Universitetet I Tromsø—Norges Arktiske Universitet Use of a N-acetylneuraminate lyase derived from the bacterium Aliivibrio salmonicida in the production of neuraminic acid and derivatives thereof
CN104059872B (zh) * 2014-07-16 2017-01-11 华东理工大学 高产n-乙酰氨基葡萄糖代谢工程菌及其构建方法和应用
CN104988108A (zh) * 2015-05-27 2015-10-21 武汉中科光谷绿色生物技术有限公司 一种高产n-乙酰神经氨酸代谢工程菌及构建方法和应用
CA3048521A1 (en) 2016-12-27 2018-07-05 Inbiose N.V. In vivo synthesis of sialylated compounds
CN108265094B (zh) * 2017-01-04 2021-03-19 河南科技学院 一种α-2，3脱氨基唾液酸乳果糖制备方法
EP3473644A1 (en) * 2017-10-17 2019-04-24 Jennewein Biotechnologie GmbH Fermentative production of n-acetylneuraminic acid
EP3486326A1 (en) 2017-11-21 2019-05-22 Jennewein Biotechnologie GmbH Method for the purification of n-acetylneuraminic acid from a fermentation broth
CN110812370A (zh) * 2018-08-14 2020-02-21 皇家燕窝私人有限公司 一种使用定制设备用于燕窝提取物的提取方法
CN112553120B (zh) * 2020-12-25 2021-09-14 华熙生物科技股份有限公司 一株大肠埃希氏菌及其在产聚唾液酸中的应用
CN113122491B (zh) * 2021-03-26 2022-08-02 清华大学 一种产n-乙酰神经氨酸的重组微生物及其应用
CN113249285A (zh) * 2021-05-13 2021-08-13 浙江工业大学 一种高产n-乙酰神经氨酸的重组枯草芽孢杆菌及其构建方法、应用
WO2023058772A1 (ja) 2021-10-08 2023-04-13 協和発酵バイオ株式会社 Ｎ－アセチルノイラミン酸及び／又はｎ－アセチルノイラミン酸含有糖質の生産能を有する微生物及び該微生物を用いたｎ－アセチルノイラミン酸及び／又はｎ－アセチルノイラミン酸含有糖質の製造方法
CN116120380A (zh) * 2021-11-15 2023-05-16 润佳(上海)医药工程有限公司 葡萄糖胺衍生物的晶型、制备方法及用途
CN115125278B (zh) * 2022-08-25 2022-11-18 山东合成远景生物科技有限公司 一种生产聚唾液酸用的补料液及聚唾液酸制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2076601B1 (en) * 2006-10-03 2011-03-30 Centre National De La Recherche Scientifique -Cnrs- High yield production of sialic acid (neu5ac) by fermentation

Also Published As

Publication number Publication date
KR20090077782A (ko) 2009-07-15
JP2010517509A (ja) 2010-05-27
BRPI0715253A2 (pt) 2013-06-04
RU2009113700A (ru) 2010-11-10
US20140273162A1 (en) 2014-09-18
US9243240B2 (en) 2016-01-26
US8722365B2 (en) 2014-05-13
WO2008097366A3 (en) 2009-03-26
US20080153133A1 (en) 2008-06-26
EP2082029A2 (en) 2009-07-29
AU2007346659A1 (en) 2008-08-14
CN101809143A (zh) 2010-08-18
AU2007346659A8 (en) 2009-05-21
WO2008097366A2 (en) 2008-08-14

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