CN117357606A - 提高免疫力的蒙医药浴组合物、制备方法及其使用方法 - Google Patents
提高免疫力的蒙医药浴组合物、制备方法及其使用方法 Download PDFInfo
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- Medicines Containing Plant Substances (AREA)
Abstract
本发明涉及中药保健品领域，公开了一种提高免疫力的蒙医药浴组合物、制备方法及其使用方法，包括以下质量份数的原料：小白蒿10‑30份、刺柏叶5‑20份、照山白3‑15份、水柏枝3‑25份、麻黄1‑10份、黄精1‑6份、麦冬1‑8份、天花粉1‑8份、玉竹1‑8份、紫茉莉粉1‑8份、蒺藜1‑8份。制备方法：称取上述原料分别磨成400目以上的粉末，然后混合在一起，加水混合均匀，大火煮至沸腾，转成小火，煎煮25‑60min，关火，即得蒙医药浴组合物。本发明在主方“五味甘露”的基础上，以黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜为辅，诸药配合，共同奏效，具有安神、补气、升阳的功效，用于提高机体免疫力。
Description
技术领域
本发明涉及中药保健品领域，特别涉及一种提高免疫力的蒙医药浴组合物、制备方法及其使用方法。
背景技术
免疫系统是人体天然的防御机制，免疫系统可帮助人体抵抗外来病毒、细菌的感染，为人体的第一道防线，提高免疫力对人体防病抗病非常关键。现有的提高免疫力的药物大都是口服的，口服的药物长期使用会对人体造成损伤，尤其是对肝肾损伤较大。
药浴是蒙医传统疗法中浸浴疗法的一种，属于现代医学物理疗法的范畴。蒙医药浴是经水为媒介物刺激来改变外界环境，并通过神经一体液将温度、机械及药物刺激人体，影响人体皮肤、心血管系统、肌肉系统、血液成分、泌尿功能、新陈代谢、神经系统来治疗疾病，从而达到治疗疾病的目的。
五味甘露药浴是蒙医浸浴疗法的一种，是在主药方剂“五味甘露”的基础上加减药味进行药浴的方法，又称阿尔山疗法。根据蒙医学理论，五味甘露具有祛寒、除湿、清热解毒、活血化瘀、益肾壮骨等功效，用以治疗四肢僵直或拘挛，胃火衰败，脾血不足，肾脏寒症赫依（气），外症汤疮以及皮肤疾病等症颇有良效。
目前已知的蒙药五味甘露药浴未发现有专门用于提高免疫力的组方，因此亟需开发一种提高免疫力的药浴组合物。
发明内容
本发明的目的在于克服现有技术的不足之处，提供一种提高免疫力的蒙医药浴组合物、制备方法及其使用方法，在主药方剂“五味甘露”的基础上，辅以黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜配伍而成，具有安神、补气、升阳的功效。
本发明解决技术问题所采用的技术方案是：
本发明公开了一种提高免疫力的蒙医药浴组合物，所述蒙医药浴组合物由以下质量份数的原料制成：小白蒿10-30份、刺柏叶5-20份、照山白3-15份、水柏枝3-25份、麻黄1-10份、黄精1-6份、麦冬1-8份、天花粉1-8份、玉竹1-8份、紫茉莉粉1-8份、蒺藜1-8份。
进一步地，所述蒙医药浴组合物由以下质量份数的原料制成：小白蒿15-30份、刺柏叶5-15份、照山白5-10份、水柏枝5-15份、麻黄1-5份、黄精1-2份、麦冬1-2份、天花粉1-2份、玉竹1-2份、紫茉莉粉1-2份、蒺藜1-2份。
进一步地，所述蒙医药浴组合物制备成药浴包、药浴液、熏蒸液或淹渍药包。
提高免疫力的蒙医药浴组合物的制备方法，包括以下步骤：按照比例称取小白蒿、刺柏叶、照山白、水柏枝、麻黄、黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜，分别磨成400目以上的粉末，然后混合在一起，加入总粉末质量5-30倍的水，混合均匀，大火煮至沸腾，转成小火，煎煮25-60min，关火，即得蒙医药浴组合物。
提高免疫力的蒙医药浴组合物的使用方法，将所述蒙医药浴组合物倒入装有200~300斤水的浴缸中，浸泡温度为40~50℃，每次浸泡时间20~30min。
本发明所用组分的来源、性、味及功效：
五味甘露中，小白蒿为菊科艾属植物冷蒿，性温，辛，燥湿，杀虫，用于胆囊炎，驱蛔虫，蛲虫；此为平地甘露；
刺柏叶是柏科植物杜松的嫩枝叶，性凉，味苦、涩，具有清肾热、祛腐生肌、治皮肤病的功效；此为阳山甘露；
照山白为杜鹃花科植物小花杜鹃的枝叶，性温，辛酸，具有温中开胃、止咳祛痰、调元滋补、祛风通络、调经止痛的功效，治，/>，产后/>，/>；此为阴山甘露；
水柏枝为柽柳科植物水柏枝的嫩枝，性凉、钝、重，治麻疹不透，风湿痹痛，癣；此为水甘露；
麻黄为麻黄科草本类小灌木植物，性温，辛，微苦，归肺、膀胱经，具有发汗散寒，宣肺平喘，利水消肿的功效；此为草甸甘露；
黄精为天门冬科黄精属多年生草本，性平、甘，具有补气养阴，健脾润肺，壮筋骨益精髓的作用，临床用于治疗脾胃虚弱，体倦乏力，口干食少，荣气不清，久风入脉的风湿痹痛；本方取其健脾润肺之功效。
麦冬，天门冬科沿阶草属草本植物，味甘、微苦，性微寒，归胃、肺、心经，有养阴润肺、益胃生津、清心除烦的功效，用于肺燥干咳、阴虚痨嗽、喉痹咽痛、津伤口渴、内热消渴、心烦失眠、肠燥便秘等症。本方取其益胃生津之功效。
天花粉为葫芦科植物栝蒌的根，性辛而润，具有清热泻火，生津止渴，排脓消肿的功效。主治：治热病口渴、消渴、黄疸、肺燥咳血、痈肿、痔痿；本方取其排脓消肿之功效。
玉竹是天门冬科黄精属多年生草本植物，补中益气，可用于治疗燥热咳嗽，津伤口渴，阴虚外感，头痛身热等；本方取其补中益气之功效。
紫茉莉是紫茉莉科紫茉莉属草本植物，具有清热解毒、活血调经、杀菌消炎、滋补的功效；本方取其杀菌消炎之功效。
蒺藜：辛微温、味苦，具有平肝阳、活血祛风、明目、止痒的功效，临床常用来治疗皮肤瘙痒、风疹，头晕、头疼等症。本方取其潜阳之功效。
本发明在主方“五味甘露”的基础上，以黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜配伍而成，其中“五味甘露”方中刺柏叶主治肾病，照山白祛除痰邪，水柏枝清毒，麻黄降肝热，小白蒿消肿燥黄水，具有祛痰化湿、清热解毒、活血化瘀益肾壮腰等功效。在主方“五味甘露”的功效基础上辅以黄精健脾润肺之功效，麦冬益胃生津之功效、天花粉排脓消肿之功效、玉竹补中益气之功效、紫茉莉粉杀菌消炎之功效、蒺藜潜阳之功效，诸药配合，使药性更温和，共同奏效，具有安神、补气、升阳的功效，可用于提高机体免疫力。
本发明的优点和积极效果是：
本发明在主方“五味甘露”的基础上，辅以黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜，诸药配合，相辅相成，协同作用，共同奏效，使其具有安神、补气、升阳的功效，可用于提高机体免疫力。
具体实施方式
下面通过具体实施例对本发明作进一步详述，以下实施例只是描述性的，不是限定性的，不能以此限定本发明的保护范围。
实施例1
一种提高免疫力的蒙医药浴组合物，所述蒙医药浴组合物由以下质量份数的原料制成：小白蒿15份、刺柏叶5份、照山白5份、水柏枝5份、麻黄1份、黄精1份、麦冬1份、天花粉1份、玉竹1份、紫茉莉粉1份、蒺藜1份。
所述的提高免疫力的蒙医药浴组合物的制备方法，包括以下步骤：按上述质量份数称取小白蒿、刺柏叶、照山白、水柏枝、麻黄、黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜，分别磨成400目以上的粉末，然后将粉末混合在一起，加入10公斤水，大火煮至沸腾，转成小火，煎煮30min，关火，即得蒙医药浴组合物。
所述的提高免疫力的蒙医药浴组合物的使用方法，将所述蒙医药浴组合物，倒入装有200斤水的浴缸中，浸泡温度为45℃，每次浸泡时间30min，第一个月浸泡7天，第二个月浸泡7天，第三个月浸泡7天，21天一个疗程，每天入浴1～2次，可提高机体免疫力。
实施例2
一种提高免疫力的蒙医药浴组合物，所述蒙医药浴组合物由以下质量份数的原料制成：小白蒿20份、刺柏叶10份、照山白8份、水柏枝10份、麻黄2份、黄精2份、麦冬2份、天花粉2份、玉竹2份、紫茉莉粉2份、蒺藜2份。
所述的提高免疫力的蒙医药浴组合物的制备方法和使用方法同实施例1。
实施例3
一种提高免疫力的蒙医药浴组合物，所述蒙医药浴组合物由以下质量份数的原料制成：小白蒿30份、刺柏叶15份、照山白10份、水柏枝15份、麻黄5份、黄精2份、麦冬2份、天花粉2份、玉竹2份、紫茉莉粉2份、蒺藜2份。
所述的提高免疫力的蒙医药浴组合物的制备方法和使用方法同实施例1。
对比例1
一种提高免疫力的蒙医药浴组合物，组方同实施例1相同，区别仅在于用枸杞代替黄精，制备方法和使用方法同实施例1相同。
对比例2
一种提高免疫力的蒙医药浴组合物，组方同实施例1相同，区别仅在于用白术代替天花粉，制备方法和使用方法同实施例1相同。
对比例3
一种提高免疫力的蒙医药浴组合物，组方同实施例1相同，区别仅在于用铁苋菜粉代替紫茉莉粉，制备方法和使用方法同实施例1相同。
对比例4
一种提高免疫力的蒙医药浴组合物，组方为小白蒿15份、刺柏叶5份、照山白5份、水柏枝5份、麻黄1份，制备方法和使用方法同实施例1相同。
功效验证
在短期内，提升机体免疫力的功效不容易区分出来，本发明的蒙医药浴组合物有效性的实验方法，选取8组小白鼠，每组10只，第1至3组小白鼠分别对应实施例1至3，第4至7组小白鼠分别对应对比例1至4，第8组为空白对照组，为纯水，8组小白鼠分别在上述对应的蒙医药浴组合物中进行泡浴，泡浴中将小白鼠固定好，确保其正常呼吸，连续泡浴45天，每天1次，然后对小白鼠进行增强免疫力功能的检验。检验采用如下两种方法：
1、ConA诱导小鼠脾淋巴细胞转化实验（MTT法）
1.1实验试剂及设备
RPMI1640细胞培养液、小牛血清、2-巯基乙醇（2-ME）、青霉素、链霉素、刀豆蛋白A（ConA）、盐酸、异丙醇、MTT、Hank's液、PBS缓冲液（pH7.2-7.4）纱布、24孔培养板，手术器械、二氧化碳培养箱、酶标仪、721分光光度计、超净工作台、高压灭菌器、无菌滤器等。
1.2实验原理
当T淋巴细胞受ConA刺激后母细胞发生增殖反应，活细胞特别是增殖细胞中的线粒体水解酶可将MTT分解为兰紫色结晶，通过测定其光密度值能反映细胞的增殖情况。每组选取三只小白鼠，取脾，分别制备脾细胞悬液，然后进行淋巴细胞增殖反应，淋巴细胞增殖反应能力越强，免疫力越好，酶标仪测定的淋巴细胞增殖能力实验结果见表1。
2、DNFB诱导小鼠迟发型变态反应实验（耳肿胀法）
2.1实验试剂及设备
DNFB、丙酮、麻油、硫化钡、打孔器
2.2实验原理及方法
二硝基氟苯（DNFB）是常用的致敏剂，二硝基氟苯稀释液可与腹壁皮肤蛋白结合成完全抗原，由此刺激T淋巴细胞增殖成致敏淋巴细胞，将进行泡浴后的小白鼠左耳涂抹二硝基氟苯稀释液，使局部肿胀，右耳不做处理，涂抹48小时后进行左右耳称重，左右耳重量差值越大，免疫增强效果越好，左右耳重量差值实验结果见表1。
表1 淋巴细胞增殖能力及左右耳重量差值实验数据
根据表1所示的ConA诱导小鼠脾淋巴细胞转化实验的淋巴细胞增殖能力测定结果可以看出，对小白鼠进行实施例1至3的蒙医药浴组合物进行泡浴，淋巴细胞增值能力比对比例1至3的增殖能力均高很多，说明在主药五味甘露的基础上辅以黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜后，可以很好的提高小白鼠的免疫力。而对小白鼠进行对比例1至3的药浴组合物进行泡浴，虽然比对比例4的淋巴细胞增值能力有一定程度的提高，但是没有实施例1至3的淋巴细胞增值能力好，也就是说对辅药中的成分进行替换后，不能与主药起到很好的协同作用，因此提高免疫力的效果不是很理想，而空白组的实验结果表明，采用纯水进行泡浴的小白鼠的细胞增殖能力比实施例1至3，对比例1至4的小白鼠的细胞增殖能力差，由此可以表明本发明药方具有很好的增强免疫力的效果。
根据表1所示的DNFB诱导小鼠迟发型变态反应实验（耳肿胀法）的左右耳重量差值实验数据可以看出，对小白鼠进行实施例1至3的蒙医药浴组合物进行泡浴后，左右耳重量差值比对比例1至3的蒙医药浴组合物进行泡浴的差值大，左右耳重量差值越大免疫力越好，说明在主药五味甘露的基础上辅以黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜后，可以很好的提高小白鼠的免疫力。而对小白鼠进行对比例1至3的蒙医药浴组合物进行泡浴，虽然比对比例4的左右耳重量差值大，但是没有实施例1至3的左右耳重量差值大，也就是说对辅药中的成分进行替换后，不能与主药起到很好的协同作用，因此提高免疫力的效果不是很理想，空白组的左右耳重量差值，比实施例1至3，对比例1至4的左右耳重量差值均低，说明免疫增强效果最差，由此可以表明本发明药方具有很好的增强免疫力的效果。
以上所述的仅是本发明的优选实施方式，应当指出，对于本领域的普通技术人员来说，在不脱离发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。
Claims (5)
1.一种提高免疫力的蒙医药浴组合物，其特征在于，所述蒙医药浴组合物由以下质量份数的原料制成：小白蒿10-30份、刺柏叶5-20份、照山白3-15份、水柏枝3-25份、麻黄1-10份、黄精1-6份、麦冬1-8份、天花粉1-8份、玉竹1-8份、紫茉莉粉1-8份、蒺藜1-8份。
2.根据权利要求1所述的提高免疫力的蒙医药浴组合物，其特征在于，所述蒙医药浴组合物由以下质量份数的原料制成：小白蒿15-30份、刺柏叶5-15份、照山白5-10份、水柏枝5-15份、麻黄1-5份、黄精1-2份、麦冬1-2份、天花粉1-2份、玉竹1-2份、紫茉莉粉1-2份、蒺藜1-2份。
3.根据权利要求1或2所述的提高免疫力的蒙医药浴组合物，其特征在于，所述蒙医药浴组合物制备成药浴包、药浴液、熏蒸液或淹渍药包。
4.一种权利要求1或2所述的提高免疫力的蒙医药浴组合物的制备方法，其特征在于，包括以下步骤：按照比例称取小白蒿、刺柏叶、照山白、水柏枝、麻黄、黄精、麦冬、天花粉、玉竹、紫茉莉粉、蒺藜，分别磨成400目以上的粉末，然后混合在一起，加入总粉末质量5-30倍的水，混合均匀，大火煮至沸腾，转成小火，煎煮25-60min，关火，即得蒙医药浴组合物。
5.一种根据权利要求1或2所述的提高免疫力的蒙医药浴组合物的使用方法，其特征在于，将所述蒙医药浴组合物倒入装有200~300斤水的浴缸中，浸泡温度为40~50℃，每次浸泡时间20~30min。
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