CN1167152A - 培养有甲醇代谢途径的微生物的方法 - Google Patents
培养有甲醇代谢途径的微生物的方法 Download PDFInfo
- Publication number
- CN1167152A CN1167152A CN97110036.5A CN97110036A CN1167152A CN 1167152 A CN1167152 A CN 1167152A CN 97110036 A CN97110036 A CN 97110036A CN 1167152 A CN1167152 A CN 1167152A
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- CN
- China
- Prior art keywords
- methyl alcohol
- substratum
- methanol
- speed
- dissolved oxygen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
加入速率(ml/L·h) | OD600 | Kex2活性(kU/ml上清液) |
2.25 | 284 | 1260 |
4.5 | 353 | 2850 |
DCW(g/L) | MeOH消耗速率(ml/g DCW·h) | |||
MeOH加入速率(ml/L.h) | 2.25 | 4.5 | 2.25 | 4.5 |
培养时间(h) | ||||
6 | 0.2 | 0.5 | 4.24 | 1.77 |
12 | 2.4 | 3.3 | 0.10 | 0.05 |
18 | 12. | 13 | 0.11 | 0.09 |
24 | 28 | 31 | 0.09 | 0.16 |
30 | 43 | 48 | 0.05 | 0.09 |
36 | 44 | 59 | 0.05 | 0.08 |
42 | 60 | 71 | 0.04 | 0.06 |
48 | 68 | 85 | 0.03 | 0.05 |
Claims (15)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP70899/1996 | 1996-03-04 | ||
JP70899/96 | 1996-03-04 | ||
JP07089996A JP3794748B2 (ja) | 1996-03-04 | 1996-03-04 | メタノール代謝系を有する微生物の培養法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1167152A true CN1167152A (zh) | 1997-12-10 |
CN1205337C CN1205337C (zh) | 2005-06-08 |
Family
ID=13444848
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN97110036.5A Expired - Fee Related CN1205337C (zh) | 1996-03-04 | 1997-03-03 | 培养有甲醇代谢途径的微生物的方法 |
Country Status (11)
Country | Link |
---|---|
US (1) | US6171828B1 (zh) |
EP (1) | EP0794256B1 (zh) |
JP (1) | JP3794748B2 (zh) |
CN (1) | CN1205337C (zh) |
AT (1) | ATE317442T1 (zh) |
AU (1) | AU733585B2 (zh) |
CA (1) | CA2198965C (zh) |
DE (1) | DE69735224T2 (zh) |
DK (1) | DK0794256T3 (zh) |
ES (1) | ES2253767T3 (zh) |
TW (1) | TW514666B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103060411A (zh) * | 2013-01-05 | 2013-04-24 | 义马煤业集团煤生化高科技工程有限公司 | 一种甲醇蛋白肽的生产方法 |
CN108603213A (zh) * | 2015-12-22 | 2018-09-28 | 合成生物制品公司 | 酵母细胞 |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2686899B1 (fr) * | 1992-01-31 | 1995-09-01 | Rhone Poulenc Rorer Sa | Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant. |
JP3794748B2 (ja) * | 1996-03-04 | 2006-07-12 | 第一アスビオファーマ株式会社 | メタノール代謝系を有する微生物の培養法 |
US20050100991A1 (en) * | 2001-04-12 | 2005-05-12 | Human Genome Sciences, Inc. | Albumin fusion proteins |
JP2003530847A (ja) * | 2000-04-12 | 2003-10-21 | ヒューマン ゲノム サイエンシズ インコーポレイテッド | アルブミン融合タンパク質 |
US20030143191A1 (en) * | 2001-05-25 | 2003-07-31 | Adam Bell | Chemokine beta-1 fusion proteins |
EP1463751B1 (en) | 2001-12-21 | 2013-05-22 | Human Genome Sciences, Inc. | Albumin fusion proteins |
WO2003059934A2 (en) * | 2001-12-21 | 2003-07-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
CN102533910B (zh) | 2003-08-01 | 2014-12-10 | 美国政府(由卫生和人类服务部、国立卫生研究院的部长所代表) | 免疫毒素的表达和纯化方法 |
WO2014071289A1 (en) * | 2012-11-05 | 2014-05-08 | Genomatica, Inc. | Microorganisms for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 3-hydroxyisobutyrate |
CN103045494B (zh) * | 2013-01-05 | 2014-10-29 | 义马煤业集团煤生化高科技工程有限公司 | 一株高效转化甲醇生产单细胞蛋白的毕赤酵母及其应用 |
WO2023184539A1 (zh) * | 2022-04-02 | 2023-10-05 | 武汉远大弘元股份有限公司 | 一种甲醇诱导启动子及其在制备丙谷二肽中的应用 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
JPH0427384A (ja) * | 1990-05-21 | 1992-01-30 | Central Glass Co Ltd | 蟻酸脱水素酵素の製造法 |
US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
WO1992013951A1 (en) * | 1991-02-04 | 1992-08-20 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Production of human serum albumin in methylotrophic yeast cells |
CA2058820C (en) * | 1991-04-25 | 2003-07-15 | Kotikanyad Sreekrishna | Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells |
ES2093294T3 (es) | 1992-02-28 | 1996-12-16 | Suntory Ltd | Nuevo vector que comprende un promotor inducible por el metanol y/o glicerol. |
GB9402832D0 (en) * | 1994-02-15 | 1994-04-06 | Smith Kline Beecham Biolog S A | Novel process |
JP3794748B2 (ja) * | 1996-03-04 | 2006-07-12 | 第一アスビオファーマ株式会社 | メタノール代謝系を有する微生物の培養法 |
-
1996
- 1996-03-04 JP JP07089996A patent/JP3794748B2/ja not_active Expired - Fee Related
-
1997
- 1997-03-03 CN CN97110036.5A patent/CN1205337C/zh not_active Expired - Fee Related
- 1997-03-03 CA CA002198965A patent/CA2198965C/en not_active Expired - Fee Related
- 1997-03-04 DK DK97301428T patent/DK0794256T3/da active
- 1997-03-04 US US08/811,024 patent/US6171828B1/en not_active Expired - Fee Related
- 1997-03-04 AU AU15038/97A patent/AU733585B2/en not_active Ceased
- 1997-03-04 TW TW086102697A patent/TW514666B/zh not_active IP Right Cessation
- 1997-03-04 EP EP97301428A patent/EP0794256B1/en not_active Expired - Lifetime
- 1997-03-04 DE DE69735224T patent/DE69735224T2/de not_active Expired - Fee Related
- 1997-03-04 AT AT97301428T patent/ATE317442T1/de not_active IP Right Cessation
- 1997-03-04 ES ES97301428T patent/ES2253767T3/es not_active Expired - Lifetime
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103060411A (zh) * | 2013-01-05 | 2013-04-24 | 义马煤业集团煤生化高科技工程有限公司 | 一种甲醇蛋白肽的生产方法 |
CN103060411B (zh) * | 2013-01-05 | 2014-02-05 | 义马煤业集团煤生化高科技工程有限公司 | 一种甲醇蛋白肽的生产方法 |
CN108603213A (zh) * | 2015-12-22 | 2018-09-28 | 合成生物制品公司 | 酵母细胞 |
CN108603213B (zh) * | 2015-12-22 | 2022-05-03 | 合成生物制品有限责任公司 | 酵母细胞 |
Also Published As
Publication number | Publication date |
---|---|
DK0794256T3 (da) | 2006-06-12 |
JP3794748B2 (ja) | 2006-07-12 |
ES2253767T3 (es) | 2006-06-01 |
AU1503897A (en) | 1997-09-11 |
TW514666B (en) | 2002-12-21 |
ATE317442T1 (de) | 2006-02-15 |
CA2198965C (en) | 2007-05-15 |
EP0794256A3 (en) | 1998-06-17 |
DE69735224T2 (de) | 2006-08-03 |
EP0794256A2 (en) | 1997-09-10 |
US6171828B1 (en) | 2001-01-09 |
DE69735224D1 (de) | 2006-04-20 |
JPH09234059A (ja) | 1997-09-09 |
CA2198965A1 (en) | 1997-09-04 |
AU733585B2 (en) | 2001-05-17 |
EP0794256B1 (en) | 2006-02-08 |
CN1205337C (zh) | 2005-06-08 |
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Owner name: SUNTORY LTD Free format text: FORMER OWNER: SUNTORY LTD. Effective date: 20030418 |
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Effective date of registration: 20030418 Address after: Tokyo, Japan Applicant after: Daiichi Suntory Pharma Co., Ltd. Address before: Osaka Applicant before: Suntory Ltd. |
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Owner name: THE FIRST ASHBIAO PHARMACEUTICAL CO., LTD. Free format text: FORMER NAME OR ADDRESS: SUNTORY LTD |
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Address after: Tokyo, Japan Patentee after: Daiichi Asubio Pharma Co., Ltd. Address before: Tokyo, Japan Patentee before: Daiichi Suntory Pharma Co., Ltd. |
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C56 | Change in the name or address of the patentee |
Owner name: ASTERIX PHARMACEUTICAL CO., LTD. Free format text: FORMER NAME OR ADDRESS: THE FIRST ASHBIAO PHARMACEUTICAL CO., LTD. |
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CP01 | Change in the name or title of a patent holder |
Address after: Tokyo, Japan Patentee after: Asubio Pharma Co., Ltd. Address before: Tokyo, Japan Patentee before: Daiichi Asubio Pharma Co., Ltd. |
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Granted publication date: 20050608 Termination date: 20100303 |