CN115698300A - 佛司可林诱导型启动子和低氧诱导型启动子 - Google Patents

佛司可林诱导型启动子和低氧诱导型启动子 Download PDF

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Publication number
CN115698300A
CN115698300A CN202180037885.0A CN202180037885A CN115698300A CN 115698300 A CN115698300 A CN 115698300A CN 202180037885 A CN202180037885 A CN 202180037885A CN 115698300 A CN115698300 A CN 115698300A
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tga
tca
seq
tgacgtca
sequence
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Chinese (zh)
Inventor
格雷厄姆·怀特斯德
维多利亚·菲奥纳·托伦斯
迈克尔·L·罗伯茨
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AskBio Inc
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Asklepios Biopharmaceutical Inc
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Priority claimed from GBGB2005473.0A external-priority patent/GB202005473D0/en
Priority claimed from GBGB2005475.5A external-priority patent/GB202005475D0/en
Priority claimed from GBGB2101969.0A external-priority patent/GB202101969D0/en
Priority claimed from GBGB2101972.4A external-priority patent/GB202101972D0/en
Application filed by Asklepios Biopharmaceutical Inc filed Critical Asklepios Biopharmaceutical Inc
Publication of CN115698300A publication Critical patent/CN115698300A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
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  • Molecular Biology (AREA)
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  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180037885.0A 2020-03-26 2021-03-26 佛司可林诱导型启动子和低氧诱导型启动子 Pending CN115698300A (zh)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US202063000155P 2020-03-26 2020-03-26
US63/000,155 2020-03-26
US202063010330P 2020-04-15 2020-04-15
GB2005475.5 2020-04-15
GBGB2005473.0A GB202005473D0 (en) 2020-04-15 2020-04-15 Forskolin-inducible promoters
GBGB2005475.5A GB202005475D0 (en) 2020-04-15 2020-04-15 Hypoxia-inducible promoters
GB2005473.0 2020-04-15
US63/010,330 2020-04-15
GB2101972.4 2021-02-12
GBGB2101969.0A GB202101969D0 (en) 2021-02-12 2021-02-12 Hypoxia inducible promoters
GBGB2101972.4A GB202101972D0 (en) 2021-02-12 2021-02-12 Forskolin inducible promoters
GB2101969.0 2021-02-12
PCT/GB2021/050743 WO2021191628A1 (en) 2020-03-26 2021-03-26 Forskolin-inducible promoters and hypoxia-inducible promoters

Publications (1)

Publication Number Publication Date
CN115698300A true CN115698300A (zh) 2023-02-03

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CN202180037885.0A Pending CN115698300A (zh) 2020-03-26 2021-03-26 佛司可林诱导型启动子和低氧诱导型启动子

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US (1) US20230374526A1 (enExample)
EP (1) EP4127180A1 (enExample)
JP (1) JP2023519925A (enExample)
CN (1) CN115698300A (enExample)
AU (1) AU2021240417A1 (enExample)
CA (1) CA3171636A1 (enExample)
WO (1) WO2021191628A1 (enExample)

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Publication number Priority date Publication date Assignee Title
WO2025015211A1 (en) * 2023-07-12 2025-01-16 Georgia Tech Research Corporation Synthetic genetic constructs that induce gene expression in response to tumor-specific metabolic alterations
WO2025090555A1 (en) * 2023-10-23 2025-05-01 University Of Southern California Ultrasound-controlled car t with sensitive evolved promoter and feedback integration and uses thereof

Citations (2)

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CN107523588A (zh) * 2017-08-18 2017-12-29 深圳大学 一种四环素慢病毒诱导表达载体及其建立方法和应用
CN110869487A (zh) * 2017-02-03 2020-03-06 马努斯生物合成股份有限公司 用于萜类化合物产物的微生物生产的代谢工程化

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GB9206874D0 (en) * 1992-03-30 1992-05-13 Connaught Lab Generation of improved inducible mammalian expression vectors
IT1258959B (it) 1992-06-09 1996-03-11 Impianto a moduli mobili per lo sviluppo e la produzione di prodotti biotecnologici su scala pilota
EP1136567A1 (en) * 2000-03-24 2001-09-26 Advanced Array Technologies S.A. Method and kit for the screening, the detection and /or the quantification of transcriptional factors
US7402406B2 (en) * 2001-10-31 2008-07-22 Astellas Pharma Inc. Methods of evaluating phosphatase inhibitors
US20060127371A1 (en) * 2002-11-25 2006-06-15 Peppelenbosch Maikel P ASB transcription repressor proteins and nucleic acids and their application in expansion of stem cells
JP2007520820A (ja) 2004-02-03 2007-07-26 エクセラーエックス， エルエルシー 製造のためのシステムおよび方法
CN101001945B (zh) 2004-06-04 2012-02-08 艾克塞勒雷克斯公司 一次性生物反应器系统及方法
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CN107523588A (zh) * 2017-08-18 2017-12-29 深圳大学 一种四环素慢病毒诱导表达载体及其建立方法和应用

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Publication number Publication date
CA3171636A1 (en) 2021-09-30
EP4127180A1 (en) 2023-02-08
JP2023519925A (ja) 2023-05-15
WO2021191628A1 (en) 2021-09-30
AU2021240417A1 (en) 2022-10-20
US20230374526A1 (en) 2023-11-23

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