CA3171636A1 - Forskolin-inducible promoters and hypoxia-inducible promoters - Google Patents

Forskolin-inducible promoters and hypoxia-inducible promoters Download PDF

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Publication number
CA3171636A1
CA3171636A1 CA3171636A CA3171636A CA3171636A1 CA 3171636 A1 CA3171636 A1 CA 3171636A1 CA 3171636 A CA3171636 A CA 3171636A CA 3171636 A CA3171636 A CA 3171636A CA 3171636 A1 CA3171636 A1 CA 3171636A1
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Canada
Prior art keywords
seq
tga
tca
tgacgtca
sequence
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Pending
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CA3171636A
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English (en)
French (fr)
Inventor
Graham WHYTESIDE
Victoria Fiona TORRANCE
Michael L. Roberts
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AskBio Inc
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Individual
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Filing date
Publication date
Priority claimed from GBGB2005473.0A external-priority patent/GB202005473D0/en
Priority claimed from GBGB2005475.5A external-priority patent/GB202005475D0/en
Priority claimed from GBGB2101969.0A external-priority patent/GB202101969D0/en
Priority claimed from GBGB2101972.4A external-priority patent/GB202101972D0/en
Application filed by Individual filed Critical Individual
Publication of CA3171636A1 publication Critical patent/CA3171636A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3171636A 2020-03-26 2021-03-26 Forskolin-inducible promoters and hypoxia-inducible promoters Pending CA3171636A1 (en)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US202063000155P 2020-03-26 2020-03-26
US63/000,155 2020-03-26
US202063010330P 2020-04-15 2020-04-15
GB2005475.5 2020-04-15
GBGB2005473.0A GB202005473D0 (en) 2020-04-15 2020-04-15 Forskolin-inducible promoters
GBGB2005475.5A GB202005475D0 (en) 2020-04-15 2020-04-15 Hypoxia-inducible promoters
GB2005473.0 2020-04-15
US63/010,330 2020-04-15
GB2101972.4 2021-02-12
GBGB2101969.0A GB202101969D0 (en) 2021-02-12 2021-02-12 Hypoxia inducible promoters
GBGB2101972.4A GB202101972D0 (en) 2021-02-12 2021-02-12 Forskolin inducible promoters
GB2101969.0 2021-02-12
PCT/GB2021/050743 WO2021191628A1 (en) 2020-03-26 2021-03-26 Forskolin-inducible promoters and hypoxia-inducible promoters

Publications (1)

Publication Number Publication Date
CA3171636A1 true CA3171636A1 (en) 2021-09-30

Family

ID=77890128

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3171636A Pending CA3171636A1 (en) 2020-03-26 2021-03-26 Forskolin-inducible promoters and hypoxia-inducible promoters

Country Status (7)

Country Link
US (1) US20230374526A1 (enExample)
EP (1) EP4127180A1 (enExample)
JP (1) JP2023519925A (enExample)
CN (1) CN115698300A (enExample)
AU (1) AU2021240417A1 (enExample)
CA (1) CA3171636A1 (enExample)
WO (1) WO2021191628A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025015211A1 (en) * 2023-07-12 2025-01-16 Georgia Tech Research Corporation Synthetic genetic constructs that induce gene expression in response to tumor-specific metabolic alterations
WO2025090555A1 (en) * 2023-10-23 2025-05-01 University Of Southern California Ultrasound-controlled car t with sensitive evolved promoter and feedback integration and uses thereof

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
GB9206874D0 (en) * 1992-03-30 1992-05-13 Connaught Lab Generation of improved inducible mammalian expression vectors
IT1258959B (it) 1992-06-09 1996-03-11 Impianto a moduli mobili per lo sviluppo e la produzione di prodotti biotecnologici su scala pilota
EP1136567A1 (en) * 2000-03-24 2001-09-26 Advanced Array Technologies S.A. Method and kit for the screening, the detection and /or the quantification of transcriptional factors
US7402406B2 (en) * 2001-10-31 2008-07-22 Astellas Pharma Inc. Methods of evaluating phosphatase inhibitors
US20060127371A1 (en) * 2002-11-25 2006-06-15 Peppelenbosch Maikel P ASB transcription repressor proteins and nucleic acids and their application in expansion of stem cells
JP2007520820A (ja) 2004-02-03 2007-07-26 エクセラーエックス， エルエルシー 製造のためのシステムおよび方法
CN101001945B (zh) 2004-06-04 2012-02-08 艾克塞勒雷克斯公司 一次性生物反应器系统及方法
US20080206817A1 (en) 2004-12-07 2008-08-28 Johann Brass Rhamnose Promoter Expression System
US20090305626A1 (en) 2005-12-05 2009-12-10 Hope Ernest G Prevalidated, modular good manufacturing practice-compliant facility
EP2207796A2 (en) * 2007-11-02 2010-07-21 Vereniging voor christelijk hoger onderwijs, wetenschappelijk onderzoek en patiëntenzorg Polypeptides involved in neuronal regeneration-associated gene expression
US8815779B2 (en) * 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
WO2011139708A2 (en) 2010-04-26 2011-11-10 Toyota Motor Engineering & Manufacturing North America, Inc. Improved hydrogen release from complex metal hydrides by solvation in ionic liquids
US10371394B2 (en) 2010-09-20 2019-08-06 Biologics Modular Llc Mobile, modular cleanroom facility
US9388373B2 (en) 2011-03-08 2016-07-12 University Of Maryland Baltimore County Microscale bioprocessing system and method for protein manufacturing
KR102128957B1 (ko) 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
MY172311A (en) * 2012-08-02 2019-11-21 Univ Putra Malaysia Hypoxia inducible factor (hif) activity reporter cell line
AU2018215537B2 (en) * 2017-02-03 2023-11-09 Manus Bio, Inc. Metabolic engineering for microbial production of terpenoid products
CN110582574A (zh) * 2017-03-13 2019-12-17 麻省理工学院 合成启动子
CN107523588B (zh) * 2017-08-18 2020-08-07 深圳大学 一种四环素慢病毒诱导表达载体及其建立方法和应用
GB201713545D0 (en) * 2017-08-23 2017-10-04 Synpromics Ltd Expression control using a regulatable intron
WO2021158658A1 (en) * 2020-02-04 2021-08-12 The Board Of Trustees Of The Leland Stanford Junior University Split crispr-cas for biological signal integration

Also Published As

Publication number Publication date
EP4127180A1 (en) 2023-02-08
CN115698300A (zh) 2023-02-03
JP2023519925A (ja) 2023-05-15
WO2021191628A1 (en) 2021-09-30
AU2021240417A1 (en) 2022-10-20
US20230374526A1 (en) 2023-11-23

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