CN115414428B

CN115414428B - Traditional Chinese medicine formula for improving chicken immunity and application thereof

Info

Publication number: CN115414428B
Application number: CN202210659497.2A
Authority: CN
Inventors: 孔庆强; 朱育玮; 吴章治; 姜亚洁; 孔令敏
Original assignee: Shandong Shengyang Bioengineering Co ltd
Current assignee: Shandong Shengyang Bioengineering Co ltd
Priority date: 2022-06-13
Filing date: 2022-06-13
Publication date: 2023-09-12
Anticipated expiration: 2042-06-13
Also published as: CN115414428A

Abstract

The invention provides a traditional Chinese medicine formula for improving chicken immunity, which comprises, by weight, 500-700 parts of astragalus mongholicus, 100-300 parts of radix isatidis, 100-300 parts of radix saposhnikoviae, 100-300 parts of bighead atractylodes rhizome, 100-200 parts of radix scutellariae, 50-150 parts of medlar, 50-150 parts of eucommia ulmoides and 100-200 parts of glossy privet fruit. The traditional Chinese medicine composition is used for improving chicken immunity and is used for preventing and treating newcastle disease.

Description

Traditional Chinese medicine formula for improving chicken immunity and application thereof

Technical Field

The invention belongs to the field of veterinary medicines, and in particular relates to a traditional Chinese medicine formula for improving chicken immunity and application thereof.

Background

Newcastle disease, also known as pseudo-fowl plague or asian fowl plague, is an acute, febrile, highly contagious disease caused by newcastle disease virus, and is mainly characterized by dyspnea and neurological symptoms and serosa and mucosal hemorrhage. The disease incidence and the death rate are high, the threat to the poultry industry is serious, the world health organization is classified as A-class animal infectious diseases (animal infectious diseases needing to be notified), and the world is classified as a class of infectious diseases. Newcastle disease can be classified into a typical newcastle disease and an atypical newcastle disease according to its clinical symptoms. According to the clinical manifestations and the course of the disease, classical newcastle disease can be divided into four categories, namely most acute, subacute and chronic. The typical newcastle disease is mainly characterized by dyspnea, serosal and mucosal bleeding, and associated neurological symptoms, and a significant drop in egg production by the laying hens. Atypical newcastle disease is a special expression form of a virulent infection immune chicken flock, and is mainly characterized in that the chicken flock has a certain antibody level, after virulent invasion, the morbidity and the mortality of the chicken flock are low, and the clinical manifestations of dyspnea are mainly caused. NDV can cause changes in non-suppurative encephalitis through the blood brain barrier in the later stage of infection, causing the chicken to develop neurological symptoms in the later stage of disease. The method of periodic vaccine vaccination is mainly used for preventing and controlling newcastle disease in China, and common vaccines are mainly conventional vaccines and also comprise a few genetic engineering vaccines. Although the chickens are immunized comprehensively, the effect is not ideal, and according to epidemiological investigation results carried out by Chinese animal sanitation and epidemiology centers in recent years, the occurrence of newcastle disease in China is still quite common.

At present, veterinary antiviral drugs are forbidden on economic animals in China, but the veterinarian does not have an antiviral concept, and only has the meaning of strengthening body resistance and eliminating evil. The basic principle of antivirus of traditional Chinese medicine is strengthening vital qi to eliminate pathogenic factor, so-called vital qi internal memory, pathogenic factor can not be dried, when organism immunity is strong, the chance of infection of pathogenic factor is small, but when organism disease resistance is poor, invasion by exogenous pathogenic factor is easy. The medicine principle is to tonify middle-jiao and qi, clear away heat and toxic materials. The traditional Chinese medicine has very complex process of exerting antiviral effect in animal body, and the antiviral mechanism is mainly divided into direct virus inhibition and indirect virus inhibition. Direct inhibition of a virus is the prevention of adsorption of the virus to a host cell, inhibition of replication of the virus within the host cell, and inhibition of maturation and release of the virus. Indirect inhibition of viral proliferation is antiviral by enhancing the immune function of the body or producing interferon.

The university of Shanxi agriculture (CN 113827688A) discloses a traditional Chinese medicine composition for reducing the death rate of newcastle disease, which comprises the following raw materials in parts by mass: 100-200 parts of radix isatidis, 50-100 parts of honeysuckle, 80-150 parts of astragalus membranaceus, 50-80 parts of fructus forsythiae, 50-100 parts of dyers woad leaf, 50-80 parts of folium artemisiae argyi, 30-50 parts of rhizoma acori graminei, 10-50 parts of wrinkled gianthyssop, 10-50 parts of radix curcumae and 30-50 parts of reed rhizome. By utilizing the mutual synergy or resistance effect among the traditional Chinese medicine raw materials, the immunity, disease resistance and external pathogenic factor invasion resistance of the chickens are improved, the effects of preventing and treating the newcastle disease are achieved, the treatment effect on the newcastle disease is improved, and the death rate of the newcastle disease is reduced. The Shandong Muwang pharmaceutical technology Co., ltd (CN 108465098A) discloses a traditional Chinese medicine composition for preventing and treating poultry newcastle disease, which comprises the following raw materials: 8-12 parts of aconite, 8-12 parts of dried ginger, 15-25 parts of cyrtomium rhizome, 15-25 parts of szechuan lovage rhizome, 45-55 parts of semen lepidii, 8-12 parts of honeysuckle flower, 15-25 parts of radix bupleuri, 15-25 parts of radix salviae miltiorrhizae, 15-25 parts of dried rehmannia root, 15-25 parts of rhizoma alismatis, 10-20 parts of rhizoma atractylodis, 10-20 parts of Chinese angelica, 15-25 parts of barbed skullcap herb, 15-25 parts of subprostrate sophora, 15-25 parts of shizandra berry and 10-20 parts of liquorice. The active components or active component groups in the composition are obtained by a proper preparation method, have inactivation and inhibition effects on newcastle disease virus, and can promote the immune function of the organism to achieve the antiviral effect; thereby realizing effective treatment of newcastle disease. The two medicaments have complex component structures and poor effect in practical application.

The compound form is used for treating diseases, is one of the characteristics of traditional Chinese medicine, and the traditional Chinese medicine can be used for making up the defect of the action intensity of single traditional Chinese medicine and enhancing the efficacy on the one hand due to the synergistic effect of the compound form; on the other hand, the toxicity and side effects can be reduced, and the toxicity of certain medicaments can be weakened. The research aims at discussing and developing a traditional Chinese medicine formula for efficiently improving chicken immunity.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine formula for improving chicken immunity, which is used for preventing and treating newcastle disease.

The invention provides the following technical scheme:

a Chinese medicinal composition for improving chicken immunity comprises (by weight parts) radix astragali 500-700, radix Isatidis 100-300, radix Saposhnikoviae 100-300, atractylodis rhizoma 100-300, scutellariae radix 100-200, fructus Lycii 50-150, eucommiae cortex 50-150, and fructus Ligustri Lucidi 100-200.

Preferably, the invention provides a traditional Chinese medicine formula for improving chicken immunity, which comprises 550-650 parts of astragalus mongholicus, 150-250 parts of radix isatidis, 150-250 parts of radix saposhnikoviae, 150-250 parts of bighead atractylodes rhizome, 120-180 parts of radix scutellariae, 80-120 parts of medlar, 80-120 parts of eucommia ulmoides and 120-180 parts of glossy privet fruit.

Preferably, the invention provides a traditional Chinese medicine formula for improving chicken immunity, which comprises 600 parts of astragalus mongholicus, 200 parts of radix isatidis, 200 parts of radix saposhnikoviae, 200 parts of bighead atractylodes rhizome, 150 parts of radix scutellariae, 100 parts of medlar, 100 parts of eucommia ulmoides and 100 parts of glossy privet fruit.

The traditional Chinese medicine formula provided by the invention can be used for preparing traditional Chinese medicine oral liquid or granule forms.

The traditional Chinese medicine formula disclosed by the invention is used for improving chicken immunity. Especially for preventing and treating newcastle disease, and has therapeutic and preventing effects.

Compared with the prior art, the invention has the beneficial technical effects that:

the medicine principle of the invention is to tonify middle-jiao and qi, clear away heat and detoxify. Astragalus mongholicus has the effects of tonifying qi, activating yang, promoting fluid production, nourishing blood and the like; the astragalus contains a plurality of active ingredients such as astragalus polysaccharide, astragalus saponin, astragalus flavonoid and the like, and the astragalus polysaccharide has been widely applied to veterinary clinic, can improve animal immunity so as to enhance organism disease resistance, can obviously inhibit proliferation of newcastle disease virus, and is used as a main functional medicament in a prescription.

Radix Isatidis: has effects in clearing away heat and toxic materials, cooling blood, and relieving sore throat; has effects of resisting pathogenic microorganism, endotoxin and inflammation. The radix isatidis can prevent the virus from adhering to the cell membrane, directly act on the virus, induce the interferon to generate and play an indirect antiviral role.

Radix Saposhnikoviae has effects of relieving exterior syndrome and dispelling pathogenic wind, and can improve heat clearing effect of radix Isatidis, and is also common antimicrobial antiviral drug.

Bighead atractylodes rhizome, having warm nature and spleen and stomach meridians, is most commonly used as a tonic traditional Chinese medicine; the bighead atractylodes rhizome polysaccharide is a main active ingredient in bighead atractylodes rhizome, is also an important material basis for playing a drug effect, and can improve the production performance by improving the immunity and the anti-stress capability of poultry.

The radix Scutellariae has antiinflammatory, antiallergic, and antimicrobial effects, and can inhibit viral replication; the radix Scutellariae has medicinal and nutritional effects, and can be used for preventing livestock and fowl diseases and promoting growth.

The wolfberry fruit contains rich nutrients such as protein, amino acid, mineral substances and the like, and active ingredients such as wolfberry polysaccharide, flavonoid compounds, alkaloids, steroid compounds and the like can improve the disease resistance of livestock and poultry, enhance the immune function of organisms and reduce the influence of external stimulation on chickens.

Eucommia ulmoides contains abundant proteins, vitamins, trace elements and fatty acids, and contains bioactive components such as lignans, iridoids, phenylpropanoids, flavonoids, polysaccharides and the like. The active ingredients of eucommia ulmoides play the roles of promoting growth, improving immunity, improving antioxidant function and inhibiting bacteria in livestock and poultry production, and can also prevent and treat diseases, improve the quality of livestock and poultry products and improve the economic benefit of cultivation.

The glossy privet fruit contains biological active components such as triterpenes, flavonoids, polysaccharides, volatile oils, iridoids, phenethyl alcohol glycosides and the like, and has various biological functions such as improving the production performance of livestock and poultry, improving the antioxidant function, improving the immunity, promoting the lipid metabolism, inhibiting bacteria and the like.

The prescription provided by the invention, in particular to prescription 1.1, not only can accelerate the generation speed of antibodies, but also can obviously improve the level of antibodies generated by organisms and obviously improve the humoral immunity function of organisms.

The prescription provided by the invention, in particular to prescription 1.1, has excellent prevention and treatment effects on newcastle disease.

The mutual synergistic effect among the traditional Chinese medicine raw materials can improve the immunity of chickens, play a role in preventing and treating newcastle disease and reduce the death rate of newcastle disease.

Detailed Description

The invention is further illustrated with reference to specific embodiments.

Example 1: the traditional Chinese medicine preparation comprises the following components in parts by weight,

prescription 1: 500-700 parts of astragalus membranaceus, 100-300 parts of radix isatidis, 100-300 parts of radix sileris, 100-300 parts of bighead atractylodes rhizome, 100-200 parts of radix scutellariae, 50-150 parts of medlar, 50-150 parts of eucommia ulmoides and 100-200 parts of glossy privet fruit;

prescription 2: 500-700 parts of astragalus, 100-300 parts of radix isatidis, 100-300 parts of radix sileris, 100-300 parts of bighead atractylodes rhizome, 100-200 parts of radix scutellariae, 50-150 parts of medlar and 50-150 parts of eucommia ulmoides;

prescription 3: 500-700 parts of astragalus, 100-300 parts of radix isatidis, 100-300 parts of radix sileris, 100-300 parts of bighead atractylodes rhizome, 100-200 parts of radix scutellariae, 50-150 parts of medlar and 100-200 parts of glossy privet fruit;

prescription 4: 500-700 parts of astragalus, 100-300 parts of radix isatidis, 100-300 parts of radix sileris, 100-300 parts of bighead atractylodes rhizome, 100-200 parts of radix scutellariae, 50-150 parts of eucommia ulmoides and 100-200 parts of glossy privet fruit;

the manufacturing method comprises the following steps:

weighing the 4 medicines according to a proportion, soaking the medicines in distilled water with the volume (mL) 10 times of the mass (parts) of the medicines for 60min, boiling with strong fire, keeping boiling with slow fire for 30min, slightly cooling, and filtering with gauze; filtering, adding distilled water 10 times of the original amount, decocting twice according to the above method, mixing the three decoctions, and concentrating into 1mL medicinal liquid containing 1.5 parts crude drug.

Example 2: influence test of Chinese medicinal preparation on Newcastle disease antibody

2.1 materials

The preparation of the traditional Chinese medicine comprises the following steps:

prescription 1.1: 600 parts of astragalus, 200 parts of radix isatidis, 200 parts of divaricate saposhnikovia root, 200 parts of bighead atractylodes rhizome, 150 parts of baical skullcap root, 100 parts of medlar, 100 parts of eucommia ulmoides, and 100 parts of glossy privet fruit;

prescription 2.1: 600 parts of astragalus, 200 parts of radix isatidis, 200 parts of divaricate saposhnikovia root, 200 parts of bighead atractylodes rhizome, 150 parts of baical skullcap root, 100 parts of medlar and 100 parts of eucommia ulmoides;

prescription 3.1: 600 parts of astragalus, 200 parts of radix isatidis, 200 parts of divaricate saposhnikovia root, 200 parts of bighead atractylodes rhizome, 150 parts of baical skullcap root, 200 parts of barbary wolfberry fruit and 100 parts of glossy privet fruit;

prescription 4.1: 600 parts of astragalus, 200 parts of radix isatidis, 200 parts of divaricate saposhnikovia root, 200 parts of bighead atractylodes rhizome, 150 parts of baical skullcap root, 100 parts of eucommia ulmoides and 200 parts of glossy privet fruit;

prescription 5: 180 parts of isatis root, 80 parts of honeysuckle, 80 parts of astragalus, 60 parts of weeping forsythia, 80 parts of dyers woad leaf, 50 parts of mugwort leaf, 35 parts of grassleaf sweelflag rhizome, 20 parts of wrinkled gianthyssop herb, 40 parts of turmeric root-tuber and 40 parts of reed rhizome (a prescription in CN 113827688A).

The preparation method is the same as in example 1.

Newcastle disease LaSota attenuated vaccine: purchasing a biological vaccine factory of a Hara pharmaceutical group; newcastle disease standard antigen and positive serum are purchased from the Chinese veterinary drug institute.

Test animals: 1 day old Hailan brown cocks were purchased from Shenyang Huamei birds Co., ltd; raising in a conventional cage to 14 days old, and maintaining standard temperature, humidity, ventilation and illumination according to a conventional method.

2.2 method

Test design and grouping: the sea-blue brown cock is raised to 14 days old (average parent antibody titer is about 4log2, average weight is about 112 g), 30 animals are randomly grouped, eye and nose are respectively spotted with Newcastle disease LaSota virus vaccine for first immunization, 3 feathers/animal are added into drinking water according to 0.3ml/L, the mixture is continuously used for one week, and a control group is conventional drinking water; each neck at 28 days of age was subcutaneously injected with newcastle disease LaSota attenuated vaccine for secondary immunization.

Determination of antibody titres in newcastle disease after immunization, blood was collected from subwinged veins at 14, 21, 28, 35, 42d for 0.5ml, centrifuged at 2000r/min for 5min to isolate serum, and single factor analysis of variance and Duncan's multiple comparisons were performed using microprombin inhibition statistical software at significant levels of P=0.05. The test results are expressed as mean ± standard deviation.

2.3 results

The effect of the Chinese medicinal preparation on newcastle disease antibody titer is shown in table 1. The test group of the Chinese medicinal preparation produced antibodies faster than the control group. And all continue to be at a higher level. All the test groups using the traditional Chinese medicine had higher antibody titers than the control group, and the test groups had significantly higher contemporaneous antibody levels at 14, 21, 28, 35, 42d after immunization than the control group (P < 0.05). Compared with formulations 2.1, 3.1, 4.1, 5, the antibody levels (Log 2) of formulations 1.1 at 14, 21, 28, 35, 42d after immunization were 7.6±0.5, 10.0±0.4, 11.2±0.4, 11.7±0.2, 11.0±0.3, respectively; and the contemporaneous antibody levels at 14, 21, 28, 35, 42d were significantly higher than other traditional Chinese medicine groups (P < 0.05) after immunization.

The test results show that the prescription provided by the invention, in particular to the prescription 1.1, can not only accelerate the generation speed of antibodies, but also obviously improve the level of antibodies generated by organisms and obviously improve the humoral immunity function of the organisms.

TABLE 1 influence of traditional Chinese medicine on Newcastle disease antibody titer (Log 2)

Group of

Dosage of

14d

21d

28d

35d

42d

Prescription 1.1

0.3ml/L

7.6±0.5

10.0±0.4

11.2±0.4

11.7±0.2

11.4±0.3

Prescription 2.1

0.3ml/L

6.5±0.7

8.4±0.5

10.3±0.6

10.0±0.4

9.8±0.5

Prescription 3.1

0.3ml/L

6.8±0.4

9.2±0.3

10.1±0.2

9.7±0.3

9.5±0.6

Prescription 4.1

0.3ml/L

6.4±0.5

8.6±0.7

10.4±0.6

10.2±0.4

10.0±0.5

Prescription 5

0.3ml/L

6.9±0.3

9.1±0.6

10.5±0.6

10.1±0.5

9.5±0.3

Control group

-

5.5±0.4

7.5±0.5

8.7±0.4

8.3±0.3

7.8±0.5

EXAMPLE 3 test of traditional Chinese medicine preparation for controlling Newcastle disease

3.1 materials

A Chinese medicinal preparation; same as in example 2

Test animals: 1 day old Hailan brown cocks were purchased from Shenyang Huamei birds Co., ltd and raised one month apart without vaccination. When the maternal antibody is reduced to 0, healthy and consistent-growth chicks are selected for testing.

3.2 method

The virus is killed by adopting a mode of injecting virus stock solution subcutaneously by adopting random grouping and 30 virus in each group. The prophylaxis group was administered on the day of challenge and the treatment group was administered after symptoms developed after virus inoculation. The two medicines are taken in the same mode in the morning and at night, the dosage of each medicine is 1.5ml of medicine liquid (oral drenching), the medicine liquid is continuously taken for one week, the number of incidences and the number of survival of each group are counted, and the survival rate is calculated.

3.2 results

The prevention and treatment effects of the traditional Chinese medicine preparation on newcastle disease are shown in table 2. Compared with the treatment effect, the traditional Chinese medicine preparation has better and excellent prevention effect, and the two have obvious difference. Compared with the formulas 2.1, 3.1, 4.1 and 5, the formula 1.1 has excellent prevention and treatment effects on newcastle disease.

Table 2 test of Chinese medicinal preparation for controlling newcastle disease

Example 4: a traditional Chinese medicine formula for improving chicken immunity comprises the following components in parts by weight.

Formula 1: 500 parts of astragalus, 300 parts of radix isatidis, 100 parts of divaricate saposhnikovia root, 100 parts of bighead atractylodes rhizome, 200 parts of baical skullcap root, 50 parts of medlar, 50 parts of eucommia ulmoides, and 200 parts of glossy privet fruit.

And 2, a formula: 700 parts of astragalus, 200 parts of radix isatidis, 200 parts of divaricate saposhnikovia root, 150 parts of bighead atractylodes rhizome, 150 parts of baical skullcap root, 100 parts of medlar, 150 parts of eucommia ulmoides and 150 parts of glossy privet fruit.

And 3, formula 3: 550 parts of astragalus, 250 parts of radix isatidis, 250 parts of divaricate saposhnikovia root, 150 parts of bighead atractylodes rhizome, 180 parts of baical skullcap root, 120 parts of medlar, 80 parts of eucommia ulmoides and 120 parts of glossy privet fruit.

And (4) a formula: 650 parts of astragalus, 180 parts of radix isatidis, 140 parts of divaricate saposhnikovia root, 180 parts of bighead atractylodes rhizome, 180 parts of baical skullcap root, 120 parts of medlar, 80 parts of eucommia ulmoides and 200 parts of glossy privet fruit.

And (5) a formula: 600 parts of astragalus, 200 parts of radix isatidis, 150 parts of divaricate saposhnikovia root, 200 parts of bighead atractylodes rhizome, 80 parts of baical skullcap root, 100 parts of medlar, 50 parts of eucommia ulmoides and 150 parts of glossy privet fruit.

The traditional Chinese medicine of the embodiment can be processed and extracted to prepare oral liquid, or can be prepared into granules, superfine powder and any pharmaceutical preparation for clinical application.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same.

Claims

1. The use of a traditional Chinese medicine formula for improving chicken immunity in preparing a preparation for preventing and treating newcastle disease is characterized by comprising the following components, by weight, 500-700 parts of astragalus mongholicus, 100-300 parts of radix isatidis, 100-300 parts of radix sileris, 100-300 parts of bighead atractylodes rhizome, 100-200 parts of radix scutellariae, 50-150 parts of wolfberry fruit, 50-150 parts of eucommia ulmoides and 100-200 parts of glossy privet fruit.

2. The use of a traditional Chinese medicine formula for improving chicken immunity according to claim 1 for preparing a preparation for preventing and treating newcastle disease, wherein 550-650 parts of astragalus membranaceus, 150-250 parts of radix isatidis, 150-250 parts of radix sileris, 150-250 parts of bighead atractylodes rhizome, 120-180 parts of radix scutellariae, 80-120 parts of medlar, 80-120 parts of eucommia ulmoides and 120-180 parts of glossy privet fruit.

3. The use of a traditional Chinese medicine formula for improving chicken immunity according to claim 2 for preparing a preparation for preventing and treating newcastle disease, wherein the traditional Chinese medicine formula is characterized by 600 parts of astragalus membranaceus, 200 parts of isatis root, 200 parts of radix sileris, 200 parts of bighead atractylodes rhizome, 150 parts of scutellaria baicalensis, 100 parts of medlar, 100 parts of eucommia ulmoides and 100 parts of glossy privet fruit.

4. The use according to any one of claims 1-3, characterized in that it is prepared in the form of a traditional Chinese medicine oral liquid or granules.