CN115176688B - Method for imitating wild planting of dendrobium - Google Patents

Abstract

The invention discloses a method for imitating wild planting of Dendrobium spp., which comprises the following steps: 1) placing the tissue-cultured seedlings of Dendrobium spp. loaded in a glass culture bottle in a shading degree of 55% to 65% and a humidity of 40%. ～70%, the temperature is 20～25 ℃ under the condition of natural light to harden seedlings, first close bottle hardening 12～18 days, open bottle mouth, continue hardening 3～5 days; Transplant to seedling trays equipped with seedling training substrates, place the seedlings under the conditions of 60% to 70% shading, 40% to 70% humidity, and 15 to 25°C for 1 to 6 months of training Obtain the domesticated seedlings; 3) plant the domesticated seedlings on the stone wall of the wild planting site through the seedling raising bag. The method for imitating wild planting of the dendrobium bentuli of the present invention improves the yield and medicinal value of the field colonization of the bent stem dendrobium.

Description

Method for imitating wild planting of dendrobium

technical field

The invention relates to the technical field of dendrobium planting. More specifically, the present invention relates to a method for imitating wild planting of Dendrobium bentuli.

Background technique

Dendrobium dendrobii is an epiphytic herb of the genus Dendrobium orchidaceae, plant height 4-10cm, stem cylindrical, slightly curved, with several nodes, 2-4 leaves, alternate on the upper part of the stem, nearly leathery, 1 flower -2 flowers, petals yellowish green below, nearly lavender above, lip yellowish, apex edge lavender. Dendrobium candidum is mainly produced in Shennongjia, Hubei, Ganluo, Sichuan and other places, and is born on rocks in valleys at an altitude of 1200-2000m. The stem of Dendrobium candidum is used as medicine, which has the functions of nourishing yin and yang, nourishing the heart and generating blood, and nourishing the liver and kidney. Dendrobium curved stem also has a very high ornamental value. The flowers are elegant, exquisite and lovely, with bright colors and fragrant smell. It is known as one of the "four major ornamental foreign flowers". It can be used as cut flowers or potted plants.

Dendrobium candidum grows in the inaccessible, cloud-shrouded cliffs and rock crevices and on towering ancient trees. The distribution range is narrow and the population is rare. It not only has high medicinal value, but also has high ornamental value. Dendrobium nodii was originally scarce in Shennongjia, and its medicinal and ornamental value has led to a sharp decline in the population of wild Dendrobium in Shennongjia, which is on the verge of extinction. Due to the difficult development and low reproduction rate of Dendrobium candidum under natural conditions, great achievements have been made in tissue culture and breeding of Dendrobium candidum. However, if the tissue culture seedlings are directly planted in a greenhouse, it violates its natural growth law. During the planting process, a large amount of pesticides and various chemical additives are often used in order to solve the problems of diseases, insect pests and yields, resulting in the deterioration of the quality of Dendrobium candidum itself, such as pesticide residues, excessive heavy metals, and poor efficacy.

Therefore, in order to effectively restore the wild population, alleviate the pressure from nature, and ensure the utility quality of Dendrobium bentuli, it is imperative to explore a method of imitating wild planting.

Contents of the invention

The purpose of the present invention is to provide a method for imitating wild planting of Dendrobium bentuli, which can improve the yield and medicinal value of Dendrobium benthide field colonization.

In order to realize the purpose and other advantages of the present invention, a kind of method for imitating wild planting of dendrobium nodule is provided, comprising the following steps:

1) Place the dendrobium tissue-cultured seedlings loaded in glass culture bottles under the conditions of 55%-65% shading, 40%-70% humidity, and 20-25°C for hardening, and close the bottle first After 12-18 days of hardening, open the bottle and continue hardening for 3-5 days;

2) After the tissue culture seedlings are taken out of the bottle, they are transplanted into a seedling tray equipped with a seedling training substrate, and placed in a shading degree of 60% to 70%, a humidity of 40% to 70%, and a temperature of 15 to 25°C. The domesticated seedlings are cultivated for 1 to 6 months under certain conditions to obtain domesticated seedlings;

3) The domesticated seedlings are fixedly planted on the stone wall of the field planting site through the seedling raising bag.

Preferably, in the method for imitating wild planting of Dendrobium bentuli, the specific process of transplanting the tissue-cultured seedlings out of the bottle in step 2) is: taking out the seedlings of Dendrobium benthide, washing off the root agar, and placing them in carbendazim After soaking in the solution for 20-30 minutes, take it out, dry the root water until the root system is slightly white, then transplant, and water thoroughly.

Preferably, in the method of imitating wild planting of Dendrobium bentuli, the seedling training substrate is composed of fermented fruit scales, perlite and vermiculite in a mass ratio of 4:1:1, and is used after sterilization and cooling, wherein the fermented fruit scales It is obtained by fermenting the fruit scales.

Preferably, the method of imitating wild planting of Dendrobium bentuli is described, and the field planting site is a damp, ventilated, half-shady and half-sun rock wall under a dense forest;

The process of fixed planting in step 3) is: put the fixed planting substrate into the non-woven fabric seedling bag, put the domesticated seedlings, then add the fixed planting substrate, shake the non-woven seedling bag gently, so that the root system of the tissue cultured seedlings is stretched and compacted , cut off the excess non-woven fabric to expose the domesticated seedling leaves, and then nail the non-woven fabric to the stone wall.

Preferably, in the method of imitating wild planting of Dendrobium bentuli, the preparation method of the fixed planting substrate is: dilute human urine 3 to 5 times with water after fermentation, and then place the fermented fruit scales in the diluted fermented urine Stand in the middle for 5-7 days, take out the fermented fruit scales and mix them with peat soil at a mass ratio of 5:1 to obtain a fixed planting substrate, and the fermented fruit scales are obtained by fermenting the fruit scales.

Preferably, in the method of imitating wild planting of Dendrobium bentuli, the method of fermenting human urine is as follows: fermenting human urine in an environment of 20-30°C, first fermenting naturally for 1 day under non-closed conditions, and then Add kombucha liquid for 2 to 3 days of non-closed fermentation, then seal and ferment for 3 to 4 days, filter and sterilize to obtain fermented urine; the volume ratio of kombucha liquid to human urine is 1:5, kombucha liquid The preparation method is as follows: mix green tea leaves with boiling water, extract for 20-30 minutes, filter to remove slag, add white sugar, mix and dissolve, then sterilize, after cooling, inoculate kombucha fungus according to the inoculation amount of 8%-10%, and place it in fermentation The container is sealed with gauze, and cultured at 25-35° C. for 15 days to obtain the kombucha bacteria liquid. The mass ratio of green tea leaves, boiling water and white sugar is 1:100:10.

Preferably, in the method of imitating wild planting of Dendrobium bentuli, the number of seedlings planted in non-woven seedling bags on the rock surface is 5×5 clumps per square meter, and each clump is 3-5 tissue cultured seedlings.

Preferably, in the method of imitating wild planting of Dendrobium bentuli, after fixed planting, the nutrient solution is regularly sprayed, and the nutrient solution is obtained by diluting 5-7 times of human urine after fermentation.

Preferably, in the method of imitating wild planting of Dendrobium spp., in step 1), after opening the bottle mouth, the tissue-cultured seedlings of Dendrobium spp. are first placed under red and blue light for 10 days, and then continue to harden the seedlings, and cultivate them under red and blue light. The conditions are as follows: 8 hours of blue light illumination, 4 hours of red light illumination, and 12 hours of darkness alternately, the light intensity is 1500-2000 lx, and the culture temperature is 20-25°C.

Preferably, in the method of imitating wild planting of Dendrobium bentuli, after the tissue-cultured seedlings are removed from the bottle, and before transplanting and domesticating the seedlings, the roots of the tissue-cultured seedlings are placed in a glass culture bottle containing an induction solution for 2-3 days. day, and placed the tissue cultured seedlings under white light with a light intensity of 5000lx. The induction solution was composed of far-infrared ceramic powder, salicylic acid and sterile water. Add 1g of far-infrared ceramic powder and 1.5mg of salicylic acid to every 1L of sterile water. Acid; Among them, tissue culture seedlings and seedlings are cultivated for 1 to 2 months.

The present invention at least includes the following beneficial effects:

(1) placing the tissue cultured seedlings in the seedling-training substrate for domestication and cultivation, and then planting them to the stone wall of the field planting site, which can improve the field planting survival rate of the dendrobium dendrobium tissue culture seedlings, and improve the output of the bent stem dendrobium;

(2) use fermented human urine to formulate custom-made planting substrate, and dilute the fermented urine as the nutrient solution applied by subsequent management, which can promote the absorption of minerals by the plants of Dendrobium curved stems, improve the content of trace elements in Dendrobium curved stems, thereby Improve medicinal value;

(3) In the seedling hardening stage before the tissue culture seedlings are taken out of the bottle, red and blue light are used for alternate cultivation, which can internally regulate growth metabolism, promote seedling development, and strengthen the growth adaptability of seedlings, thereby improving the survival of Dendrobium candidum seedlings in the field. Rate;

(4) Before transplanting and domestication, place the tissue cultured seedlings in the induction solution. Under the strong light stimulation and the protection of salicylic acid, the far-infrared ceramic powder promotes the activation of biological macromolecules in plant cells, enhances cell vitality, and accelerates metabolism. Shorten the time for seedling training, and at the same time increase the survival rate and trace element content of Dendrobium officinale in the field.

Other advantages, objectives and features of the present invention will partly be embodied through the following descriptions, and partly will be understood by those skilled in the art through the research and practice of the present invention.

Detailed ways

The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.

It should be noted that the experimental methods described in the following examples, unless otherwise specified, are conventional methods, and the reagents and materials, unless otherwise specified, can be obtained from commercial sources.

A method for imitating wild planting of dendrobium dendrobium comprises the following steps:

1) Place the dendrobium tissue-cultured seedlings loaded in glass culture bottles under the conditions of 55%-65% shading, 40%-70% humidity, and 20-25°C for hardening, and close the bottle first After hardening for 12 to 18 days, open the bottle and continue hardening for 3 to 5 days. The tissue culture seedlings are selected from those with no disease, no mold, strong rhizomes, and no mechanical damage;

2) After the tissue culture seedlings are taken out of the bottle, they are transplanted into a seedling tray equipped with a seedling training substrate, and placed in a shading degree of 60% to 70%, a humidity of 40% to 70%, and a temperature of 15 to 25°C. The domesticated seedlings can be obtained by cultivating the seedlings under certain conditions for 1 to 6 months; the seedling substrate can be mixed with fermented pine bark or fermented fruit scales, perlite and vermiculite in a certain proportion, fermented fruit scales are preferred, and the seedling substrates are used Autoclaved for 30 minutes or exposed to high temperature for 3 to 5 days before use after cooling.

3) The domesticated seedlings are fixedly planted on the stone wall of the field planting site through the seedling raising bag. Seedling hardening and seedling training can be carried out under natural light outdoors or under greenhouse conditions.

More preferably, in the method for imitating wild planting of Dendrobium bentuli, the specific process of transplanting the tissue-cultured seedlings out of the bottle in step 2) is: taking out the seedlings of Dendrobium benthum, washing off the root agar, and placing them in carbendazim After soaking in the solution for 20-30 minutes, take it out, dry the water in the roots, and then transplant until the roots turn slightly white. Put the dry tissue cultured seedlings into the seedling tray, cover the upper part with a layer of seedling training substrate, compact it lightly, and water thoroughly after the entire seedling tray is full.

More preferably, in the method of imitating wild planting of Dendrobium bentuli, the seedling training substrate is composed of fermented fruit scales, perlite and vermiculite in a mass ratio of 4:1:1, and is used after sterilization and cooling, wherein the fermented fruit scales It is obtained by fermenting the fruit scales. The fruit scales refer to the seed scales of Huashan pine. Huashan pine cones fall to the ground naturally after they mature in October to November. The size and shape of the fruit scales are uniform, with drainage and air permeability, and rich in organic matter content, which is very suitable for domestication substrates of aerial root plants such as dendrobium. Before use, fruit scales will be processed through fermentation to remove resins, cellulose, lignin, carbolic acid, tannic acid and other substances harmful to plant growth. The method of fruit scale fermentation uses fermentation aids for outdoor fermentation. The details are as follows: Add appropriate amount of urea and carbendazim to wet the detached fruit scales, then fully stir, cover with plastic film and let it stand for 1 day, then add fermentation aids Fully stir, pile up, cover with plastic film, expose to the sun for 2-3 months at high temperature, turn over once every 5 days or so, spray water when turning, keep the substrate moist, the mass ratio of fruit scales, urea, and carbendazim is 10: 2:1, the mass ratio of the mixture of the three raw materials to the fermentation aid is 10:1. The fermentation aid is purchased from an organic material decomposing agent produced by Henan Shuanghui Agricultural Science and Technology Development Co., Ltd. The main component is Bacillus subtilis , Bacillus licheniformis, lactic acid bacteria, Saccharomyces cerevisiae, Rhizopus, amylase, protease and its metabolites, etc.

More preferably, the method for imitating wild planting of Dendrobium bentuli, the field planting site is damp (humidity between 50% and 80%), ventilated, half shade and half sun (half shade and half sun refers to the forest) under the dense forest. Scattered light, shading at 50% to 70%) rock walls, before transplanting, clean up the weeds and dead branches on the rock walls.

The process of fixed planting in step 3) is: put the fixed planting substrate into the non-woven fabric seedling bag, put the domesticated seedlings, then add the fixed planting substrate, shake the non-woven seedling bag gently, so that the root system of the tissue cultured seedlings is stretched and compacted , cut off the excess non-woven fabric to expose the domesticated seedling leaves, and then nail the non-woven fabric to the stone wall.

More preferably, in the method of imitating wild planting of Dendrobium bentuli, the preparation method of the fixed planting substrate is: dilute human urine 3 to 5 times with water after fermentation, and then place the fermented fruit scales in the diluted fermented urine Stand in the middle for 5-7 days, take out the fermented fruit scale and mix them with peat soil according to the mass ratio of 5:1 to obtain the fixed planting substrate. Ferment for 10-15 days for treatment, for example: put human urine in an environment of 20-30°C for natural fermentation, first for 3-5 days under non-closed conditions, then for 7-10 days in closed fermentation, then filter and sterilize Get fermented urine. More preferably, the method of fermenting human urine is as follows: ferment human urine in an environment of 20-30°C, first ferment naturally for 1 day under non-closed conditions, and then add kombucha bacteria liquid for 2-3 days of non-closed fermentation , then closed and fermented for 3 to 4 days, filtered and sterilized to obtain fermented urine; the volume ratio of the kombucha bacterium liquid to human urine is 1:5, and the preparation method of the kombucha bacterium liquid is: mix green tea leaves with boiling water, soak Extract for 20-30 minutes, filter to remove slag, add white sugar, mix and dissolve, then sterilize, cool and inoculate kombucha fungus according to the inoculation amount of 8%-10%, put it in a fermentation container and seal it with gauze, at 25-35°C Cultivate for 15 days to obtain the kombucha bacteria liquid, and the mass ratio of green tea leaves, boiling water and white sugar is 1:100:10.

More preferably, in the method of imitating wild planting of Dendrobium nodulii, after fixed planting, the nutrient solution is regularly sprayed, and the nutrient solution is obtained by diluting human urine 5-7 times after fermentation.

Use the fermented human urine to prepare the custom-made planting medium, and dilute the fermented urine as the nutrient solution for subsequent management, which can promote the absorption of minerals by the plants of Dendrobium curved stems, increase the content of trace elements in Dendrobium curved stems, and thus improve the medicinal properties. value.

More preferably, in the method of imitating wild planting of Dendrobium nodulii, the number of non-woven seedling-raising bag seedlings planted on the rock surface is 5×5 clumps per square meter, and each clump is 3-5 tissue-cultured seedlings.

More preferably, in the method of imitating wild planting of Dendrobium candidum, in step 1), after opening the bottle mouth, the tissue cultured seedlings of Dendrobium candidum are first placed under red and blue light for 10 days, and then continue to harden the seedlings, and the red and blue light are cultivated. The conditions are as follows: 8 hours of blue light illumination, 4 hours of red light illumination, and 12 hours of darkness alternately, the light intensity is 1500-2000 lx, and the culture temperature is 20-25°C. In the seedling hardening stage before the tissue-cultured seedlings are taken out of the bottle, red and blue light are used to cultivate alternately, which can internally regulate growth metabolism, promote seedling development, and strengthen seedling growth adaptability, thereby improving the survival rate of Dendrobium candidum seedlings in the field for fixed planting.

More preferably, in the method of imitating wild planting of Dendrobium bentuli, after the tissue-cultured seedlings are removed from the bottle, and before transplanting and taming the seedlings, the roots of the tissue-cultured seedlings are placed in a glass culture bottle containing an induction solution for 2-3 days. day, and placed the tissue cultured seedlings under white light with a light intensity of 5000lx. The induction solution was composed of far-infrared ceramic powder, salicylic acid and sterile water. Add 1g of far-infrared ceramic powder and 1.5mg of salicylic acid to every 1L of sterile water. Acid; Among them, tissue culture seedlings and seedlings are cultivated for 1 to 2 months. Before transplanting and domestication, the tissue cultured seedlings are placed in the induction solution. Under the stimulation of strong light and the protection of salicylic acid, the far-infrared ceramic powder promotes the activation of biological macromolecules in plant cells, enhances cell vitality, accelerates metabolism, and shortens the time of domestication. At the same time, the survival rate and trace element content of Dendrobium candidum in field fixed planting were improved.

The technical solution of the present invention may also include the following technical details to better achieve technical effects:

The fermented fruit scales used in the following examples are all obtained by outdoor fermentation using fermentation aids, specifically as follows: add an appropriate amount of urea and carbendazim to wet the detached fruit scales, then fully stir them, cover them with a plastic film and let them stand for 1 day , then add fermentation aids and stir well, pile them up, cover with plastic film, expose to the sun at high temperature (above 30°C) for 2-3 months, turn over once every 5 days or so, spray water when turning, keep the substrate moist, fruit scales, The mass ratio of urea and carbendazim is 10:2:1, the mass ratio of the mixture of the three raw materials to the fermentation aid is 10:1, and the fermentation aid is purchased from Henan Shuanghui Agricultural Science and Technology Development Co., Ltd. Organic material decomposing agent, the main components are Bacillus subtilis, Bacillus licheniformis, lactic acid bacteria, Saccharomyces cerevisiae, rhizopus, amylase, protease and its metabolites, etc.

Example 1:

A method for imitating wild planting of dendrobium dendrobium comprises the following steps:

1) Place the dendrobium tissue-cultured seedlings loaded in glass culture bottles under the conditions of 55%-65% shading, 40%-70% humidity, and 20-25°C for hardening, and close the bottle first Seedling hardening for 12 days, open the bottle, and continue hardening for 3 days;

2) After the tissue culture seedlings are taken out of the bottle, they are transplanted into a seedling tray equipped with a seedling training substrate, and placed in a shading degree of 60% to 70%, a humidity of 40% to 70%, and a temperature of 15 to 25°C. The domesticated seedlings were cultivated for 1 month under the condition of the domesticated seedlings. The seedling substrate was composed of fermented fruit scales, perlite and vermiculite according to the mass ratio of 4:1:1. It was used after sterilization and cooling, and the fermented fruit scales were fruit scales. Obtained by fermentation;

3) The domesticated seedlings are fixedly planted on a damp, ventilated, half shade and half sun rock wall under the dense forest through the seedling bag. The half shade and half sun refer to the scattered light under the forest. Between 50% and 80%.

The specific process of transplanting the tissue cultured seedlings out of the bottle in step 2) is: take out the dendrobium dendrobium seedlings, wash off the root agar, place them in the carbendazim solution and soak them for 20 minutes, then remove them, and dry the root moisture until the roots are slightly Turn white and then transplant, water thoroughly.

The process of fixed planting in step 3) is: put the fixed planting substrate into the non-woven fabric seedling bag, put the domesticated seedlings, then add the fixed planting substrate, shake the non-woven seedling bag gently, so that the root system of the tissue cultured seedlings is stretched and compacted , cut off the excess non-woven fabric to expose the domesticated seedling leaves, and then nail the non-woven fabric to the stone wall.

The preparation method of the fixed planting substrate is as follows: after the human urine is fermented, it is diluted with water 3 times, and then the fermented fruit scales are placed in the diluted fermented urine and left to stand for 5 days, and the fermented fruit scales and peat soil are taken out in a mass ratio of 5 : 1 mixing to obtain the fixed planting substrate, and the fermented fruit scales are obtained through fermentation of the fruit scales;

The method of fermenting human urine is as follows: put the human urine to ferment naturally in an environment of 20-30°C, ferment naturally for 3 days under non-closed conditions, then ferment in closed ferment for 7 days, filter and sterilize to obtain fermented urine.

The number of seedlings planted in the non-woven seedling bag on the rock surface is 5 × 5 clumps per square meter, and each clump is 3 tissue cultured seedlings.

After the fixed planting, the nutrient solution is regularly sprayed, which is obtained by diluting 5 times of human urine after fermentation.

Example 2:

A method for imitating wild planting of dendrobium dendrobium comprises the following steps:

1) Place the dendrobium tissue-cultured seedlings loaded in glass culture bottles under the conditions of 55%-65% shading, 40%-70% humidity, and 20-25°C for hardening, and close the bottle first Seedling hardening for 18 days, open the bottle, and continue hardening for 5 days;

2) After the tissue culture seedlings are taken out of the bottle, they are transplanted into a seedling tray equipped with a seedling training substrate, and placed in a shading degree of 60% to 70%, a humidity of 40% to 70%, and a temperature of 15 to 25°C. The domesticated seedlings were cultivated for 6 months to obtain the domesticated seedlings under the above conditions. The seedling substrate was composed of fermented fruit scales, perlite and vermiculite according to the mass ratio of 4:1:1, and was used after sterilization and cooling;

3) The domesticated seedlings are fixedly planted on a damp, ventilated, half shade and half sun rock wall under the dense forest through the seedling bag. The half shade and half sun refer to the scattered light under the forest. Between 50% and 80%.

The specific process of transplanting the tissue cultured seedlings out of the bottle in step 2) is as follows: take out the dendrobium dendrobium seedlings, wash off the root agar, put them in the carbendazim solution and soak them for 30 minutes, then take them out, and dry the root moisture until the roots are slightly Turn white and then transplant, water thoroughly.

The process of fixed planting in step 3) is: put the fixed planting substrate into the non-woven fabric seedling bag, put the domesticated seedlings, then add the fixed planting substrate, shake the non-woven seedling bag gently, so that the root system of the tissue cultured seedlings is stretched and compacted , cut off the excess non-woven fabric to expose the domesticated seedling leaves, and then nail the non-woven fabric to the stone wall.

The preparation method of the fixed planting substrate is as follows: after the human urine is fermented, it is diluted 5 times with water, then the fermented fruit scales are placed in the diluted fermented urine and left to stand for 7 days, and the fermented fruit scales and peat soil are taken out in a mass ratio of 5 : 1 mixing to obtain the fixed planting substrate;

The method for fermenting human urine is as follows: put the human urine in an environment of 20-30°C for natural fermentation, first ferment naturally for 5 days under non-enclosed conditions, then ferment in closed conditions for 10 days, filter and sterilize to obtain fermented urine.

The planting quantity of non-woven seedling bag seedlings on the rock surface is 5×5 clumps per square meter, and each clump is 5 tissue cultured seedlings.

After fixed planting, the nutrient solution is regularly sprayed, which is obtained by diluting human urine 7 times after fermentation.

Example 3:

A method for imitating wild planting of dendrobium dendrobium comprises the following steps:

1) Place the dendrobium tissue-cultured seedlings loaded in glass culture bottles under the conditions of 55%-65% shading, 40%-70% humidity, and 20-25°C for hardening, and close the bottle first Seedling hardening for 15 days, open the bottle, and continue hardening for 4 days;

2) After the tissue culture seedlings are taken out of the bottle, they are transplanted into a seedling tray equipped with a seedling training substrate, and placed in a shading degree of 60% to 70%, a humidity of 40% to 70%, and a temperature of 15 to 25°C. The domesticated seedlings were cultivated for 4 months to obtain the domesticated seedlings under the above conditions. The seedling substrate was composed of fermented fruit scales, perlite and vermiculite according to the mass ratio of 4:1:1, and was used after sterilization and cooling;

3) The domesticated seedlings are fixedly planted on a damp, ventilated, half shade and half sun rock wall under the dense forest through the seedling bag. The half shade and half sun refer to the scattered light under the forest. Between 50% and 80%.

The specific process of transplanting the tissue cultured seedlings out of the bottle in step 2) is: take out the dendrobium dendrobium seedlings, wash off the root agar, place them in the carbendazim solution and soak them for 25 minutes, then remove them, and dry the root moisture until the roots are slightly Turn white and then transplant, water thoroughly.

The process of fixed planting in step 3) is: put the fixed planting substrate into the non-woven fabric seedling bag, put the domesticated seedlings, then add the fixed planting substrate, shake the non-woven seedling bag gently, so that the root system of the tissue cultured seedlings is stretched and compacted , cut off the excess non-woven fabric to expose the domesticated seedling leaves, and then nail the non-woven fabric to the stone wall.

The preparation method of the fixed planting substrate is as follows: after the human urine is fermented, it is diluted 4 times with water, then the fermented fruit scales are placed in the diluted fermented urine and left to stand for 6 days, and the fermented fruit scales and peat soil are taken out in a mass ratio of 5 : 1 mixing to obtain the fixed planting substrate;

The method for fermenting human urine is as follows: put human urine in an environment of 20-30°C for natural fermentation, firstly ferment naturally for 4 days under non-enclosed conditions, then ferment in closed conditions for 8 days, filter and sterilize to obtain fermented urine.

The number of seedlings planted in the non-woven seedling bag on the rock surface is 5×5 clumps per square meter, and each clump is 4 tissue cultured seedlings.

After fixed planting, the nutrient solution is regularly sprayed, which is obtained by diluting human urine 6 times after fermentation.

Example 4:

On the basis of Example 3, the method of imitating wild planting of Dendrobium bentuli, the method of fermenting human urine is as follows: ferment human urine under an environment of 20-30°C, and first ferment naturally under non-closed conditions 1 day, then add kombucha bacteria liquid for non-airtight fermentation for 3 days, then seal fermentation for 4 days, filter and sterilize to obtain fermented urine; the volume ratio of kombucha bacteria liquid to human urine is 1:5, kombucha bacteria liquid The preparation method is as follows: mix green tea leaves with boiling water, extract for 30 minutes, filter to remove slag, add white granulated sugar, mix and dissolve, sterilize, inoculate kombucha fungus according to the inoculation amount of 10% after cooling, place in a fermentation container and seal with gauze, The kombucha bacteria liquid is obtained by culturing at 25-35°C for 15 days, and the mass ratio of green tea leaves, boiling water and white sugar is 1:100:10.

All the other methods are the same as in Example 3.

Example 5:

On the basis of Example 3, the described method of imitating wild planting of Dendrobium candidum, after opening the bottle mouth in step 1), place the tissue cultured seedlings of Dendrobium candidum under red and blue light to cultivate for 10 days, and then continue to harden the seedlings , the red and blue light culture conditions are as follows: first blue light light for 8 hours, then red light light for 4 hours, and then dark for 12 hours, alternating in sequence, the light intensity is 2000lx, and the culture temperature is 20-25°C. All the other methods are the same as in Example 3.

Embodiment 6:

On the basis of Example 3, in the method of imitating wild planting of Dendrobium bentuli, after the tissue-cultured seedlings are taken off the bottle, before transplanting and domesticating the seedlings, the roots of the tissue-cultured seedlings are placed in a glass culture bottle equipped with an induction solution Treat for 3 days, and place the tissue cultured seedlings under white light with a light intensity of 5000lx. The induction solution is composed of far-infrared ceramic powder, salicylic acid and sterile water. Add 1g of far-infrared ceramic powder and 1.5mg of salicylic acid. Among them, the training period of tissue cultured seedlings and seedlings is 1 month. All the other methods are the same as in Example 3.

Embodiment 7:

On the basis of Example 4, the described method of imitating wild planting of Dendrobium candidum, after opening the bottle mouth in step 1), place the Dendrobium candidum tissue culture seedlings under red and blue light to cultivate for 10 days, and then continue to harden the seedlings , the red and blue light culture conditions are as follows: first blue light light for 8 hours, then red light light for 4 hours, and then dark for 12 hours, alternating in sequence, the light intensity is 2000lx, and the culture temperature is 20-25°C. All the other methods are the same as in Example 4.

Embodiment 8:

On the basis of Example 7, in the method of imitating wild planting of Dendrobium bentuli, after the tissue-cultured seedlings are taken off the bottle, before transplanting and domesticating the seedlings, the roots of the tissue-cultured seedlings are placed in a glass culture bottle equipped with an induction solution Treat for 3 days, and place the tissue cultured seedlings under white light with a light intensity of 5000lx. The induction solution is composed of far-infrared ceramic powder, salicylic acid and sterile water. Add 1g of far-infrared ceramic powder and 1.5mg of salicylic acid. Among them, the training period of tissue cultured seedlings and seedlings is 1 month. All the other methods are the same as in Example 7.

Comparative example 1:

On the basis of Example 3, the tissue-cultured seedlings were taken out of the bottle and planted in the seedling-raising bag on a damp, ventilated, half-shady and half-sun rock wall under a dense forest to remove the domestication and cultivation process. All the other methods are the same as in Example 3.

Comparative example 2:

On the basis of Example 3, the domesticated seedlings are placed in the greenhouse for planting management, and the planting management conditions in the greenhouse include: the shading degree of the greenhouse is 65%; the temperature is controlled at 15-25°C; the humidity is controlled at 40%-70% In continuous high temperature weather, water should be sprayed on the leaves of the domesticated seedlings every morning and afternoon, and the domesticated seedlings should be watered at intervals of 3-4 days to keep the substrate moist. The management before domestication seedling is the same as embodiment 3.

Comparative example 3:

On the basis of Example 3, the fermented fruit scales in the seedling training substrate and the fixed planting substrate were replaced with fermented pine bark. The fermentation method of fermented pine bark is as follows: first sterilize the pine bark, for example, boil it in boiling water for 2-3 hours to kill the bacteria and insect eggs inside, pick it up and put it in a plastic bucket, then pour it into urea solution to stir and moisturize. Then cover the lid and seal it, put it in the sun for about 2-3 months, and stir it every 5 days to make it fully decomposed. The mass ratio of urea solution to pine bark is 3:1.

In order to better highlight the beneficial effect of the imitative wild planting method of Dendrobium spp. of the present invention, the contriver adopts the tissue culture seedling of Dendrobium spp. The method of example and comparative example was used for planting experiments, and the planting site was selected on the stone wall under the dense forest next to a certain Guanmen Mountain laboratory in Shennongjia (conforming to the conditions of damp, ventilated, half shade and half sun under the dense forest, scattered light under the forest, shading The shade is between 50% and 70%, and the humidity is between 50% and 80%. Each group of examples or comparative examples uses 30 culture bottles to count the survival rate of dendrobium dendrobium field colonization and harvest yield, and measure the bent stem For the trace element content of Dendrobium, the natural growth of Dendrobium dendrobii on the stone wall of a Yinyu River gorge in Shennongjia was used as the control group (non-artificial planting). The statistical results are shown in Table 1.

Table 1. Planting test results

Can draw from Table 1, comparative example 1, embodiment 1, embodiment 2, embodiment 3 seedling cultivation time are respectively 0 month, 1 month, 6 months, 4 months, the dendrobium dendrobium that finally obtains The survival rates of fixed planting in the field are 50%, 60%, 80%, 70%, and the annual yields are 110g/m ² , 120g/m ² , 175g/m ² , 160g/m ² , and the trace elements are also correspondingly different. , which shows that the length of time for seedling training has a great influence on the growth of Dendrobium candidum seedlings after they are planted in the wild, but the time for seedling training is too long, which increases the labor of planting management and production costs, and also reduces the annual output. Compared with Example 3, Example 4 uses the human urine prepared by kombucha fermentation, and the human urine is used in the planting substrate and nutrient solution. As can be seen from Example 3 and Example 4, the results of Example 4 The method shows that the content of trace elements in Dendrobium candidum is significantly increased, and the survival rate and annual output of field fixed planting are also slightly increased, which shows that the fermented human urine is used to prepare the fixed planting substrate, and the fermented urine is diluted as the nutrition applied by subsequent management. The liquid can promote the absorption of minerals in the dendrobium dendrobium plant, increase the content of trace elements in the dendrobium dendrobium, thereby improving the medicinal value.

Compared with Example 3 and Example 7, the planting method of Example 7 has more red and blue light alternate cultivation stages. As can be seen from Table 1, in the seedling hardening stage before the tissue culture seedlings are taken off the bottle, red and blue lights are used. Alternate cultivation significantly improved the survival rate and annual output of Dendrobium nodulum seedlings in the field, and slightly increased the trace elements.

Embodiment 6 compares embodiment 3, embodiment 8 compares the planting method of embodiment 7 and all has more inducing solution treatment process, and the tissue culture seedling taming seedling of embodiment 3 and embodiment 7 is cultivated for 4 months, embodiment 6 and the tissue cultured seedlings of Example 8 were cultivated for 1 month. As can be seen from Table 1, before transplanting and domestication, the tissue cultured seedlings were placed in the induction solution for processing, which can shorten the time for seedlings and improve the curvature of the stem simultaneously. The survival rate, annual output and trace element content of Dendrobium in the field indicate that the process of induction solution treatment has a great influence on the field growth of Dendrobium curved stem. For planting Dendrobium curved stem, the planting time is greatly shortened, and the production labor and labor costs are reduced. cost.

Embodiment 3 is the field planting method of the present invention, and comparative example 2 is a greenhouse planting method. As can be seen from Table 1, the fixed planting survival rate and annual output of Dendrobium spp. The content of trace elements is slightly higher, but the cost of field planting is lower than that of greenhouse planting, and quality and safety problems such as pesticide residues and drug efficacy quality reduction that exist in the process of greenhouse planting are avoided. better.

The difference between Example 3 and Comparative Example 3 is that the raw materials of the seedling training substrate and the fixed planting substrate are replaced by fermented pine bark by fermented fruit scales. As can be seen from Table 1, the dendrobium candidum obtained by using fermented fruit scales for planting in the field survived. The rate and annual yield are higher, and the content of trace elements also increases accordingly, which shows that the fermented fruit scale is more suitable for the planting of Dendrobium bentuli than the pine bark which is commonly used for substrate production.

Although the embodiment of the present invention has been disclosed as above, it is not limited to the use listed in the specification and implementation, it can be applied to various fields suitable for the present invention, and it can be easily understood by those skilled in the art Therefore, the invention is not limited to the specific details without departing from the general concept defined by the claims and their equivalents.

Claims (5)

1. the method for imitating wild planting of dendrobium dendrobii is characterized in that, comprises the following steps: 1) Put the dendrobium tissue cultured seedlings loaded in glass culture bottles under the conditions of shading degree of 55%~65%, humidity of 40%~70%, and temperature of 20~25℃ to harden the seedlings, and close the bottle first After 12-18 days of hardening, open the bottle and continue hardening for 3-5 days; 2) After the tissue culture seedlings are taken out of the bottle, they are transplanted into a seedling tray equipped with a seedling training substrate, and placed in a shading degree of 60%~70%, a humidity of 40%~70%, and a temperature of 15~25°C The domesticated seedlings are cultivated for 1 to 2 months to obtain domesticated seedlings under certain conditions; 3) The domesticated seedlings are fixedly planted on the stone wall of the field planting site through seedling bags; the field planting site is a damp, ventilated, half-shady and half-sun rock wall under dense forests; The process of fixed planting in step 3) is: put the fixed planting substrate into the non-woven seedling bag, put the domesticated seedlings, then add the fixed planting substrate, shake the non-woven seedling bag gently, so that the root system of the tissue cultured seedlings can be stretched and compacted , cut off the excess non-woven fabric to expose the domesticated seedling leaves, and then nail the non-woven fabric to the stone wall; The preparation method of the fixed planting substrate is as follows: ferment human urine and dilute it with water 3 to 5 times, then place the fermented fruit scales in the diluted fermented urine and let it stand for 5 to 7 days, take out the fermented fruit scales and press the peat soil The mass ratio is 5:1 and mixed evenly to obtain the fixed planting substrate, and the fermented fruit scales are obtained by fermenting the fruit scales; The method of human urine fermentation is as follows: ferment human urine in an environment of 20-30°C, first ferment naturally for 1 day under non-closed conditions, then add kombucha bacteria liquid for 2-3 days of non-closed fermentation, and then seal the fermentation After 3~4 days, filter and sterilize to obtain fermented urine; the volume ratio of kombucha bacteria solution to human urine is 1:5, and the preparation method of kombucha bacteria solution is: mix green tea leaves with boiling water, and extract for 20~30min , filter to remove slag, add white granulated sugar, mix and dissolve, then sterilize, inoculate kombucha fungus according to the inoculation amount of 8%~10% after cooling, put it in a fermentation container and seal it with gauze, and cultivate it at 25~35°C for 15 days to obtain Kombucha bacterium liquid, the mass ratio of green tea leaves, boiling water and white sugar is 1:100:10; After opening the bottle mouth in step 1), place the dendrobium dendrobium tissue culture seedlings under red and blue light for 10 days, and then continue to harden the seedlings. The red and blue light culture conditions are: first blue light for 8 hours, then red light for 4 hours, and then dark 12h, alternating in turn, the light intensity is 1500~2000lx, and the culture temperature is 20~25℃; After the tissue-cultured seedlings are removed from the bottle, before transplanting and training the seedlings, the roots of the tissue-cultured seedlings are placed in a glass culture bottle filled with an induction solution for 2-3 days, and the tissue-cultured seedlings are placed under a white light with a light intensity of 5000lx , the induction solution is composed of far-infrared ceramic powder, salicylic acid and sterile water, and 1 g of far-infrared ceramic powder and 1.5 mg of salicylic acid are added to each 1 L of sterile water. 2. The method for imitating wild planting of Dendrobium bentuli as claimed in claim 1, characterized in that, in step 2), the specific process of transplanting the tissue-cultured seedlings out of the bottle is: taking out the dendrobium benthock seedlings, washing off the root agar , soaked in carbendazim solution for 20-30 minutes, then fished out, dried the root water until the root system turned slightly white, then transplanted, and watered thoroughly. 3. the method for imitating wild planting of Dendrobium dendrobii as claimed in claim 1, is characterized in that, seedling training substrate is made up of fermented fruit scales, perlite and vermiculite according to mass ratio of 4:1:1, after sterilization and cooling The fermented fruit scales are obtained by fermenting the fruit scales. 4. the method for imitating wild planting of dendrobium dendrobium as claimed in claim 1, is characterized in that, the planting quantity of non-woven seedling-growing bag seedlings on the rock surface is 5 * 5 clumps per square meter, and every clump is a group of 3-5 strains Seedlings. 5. the method for imitating wild planting of dendrobium dendrobium as claimed in claim 1, is characterized in that, after fixed planting, regularly spray nutrient solution, nutrient solution is to be diluted 5-7 times gained after the fermentation of human urine.