CN107360969A - Suitable for the culture medium and method of the wild Dendrobium flexicaule tissue culture of Mt. Funiushan In Henan Province - Google Patents
Suitable for the culture medium and method of the wild Dendrobium flexicaule tissue culture of Mt. Funiushan In Henan Province Download PDFInfo
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- CN107360969A CN107360969A CN201710619051.6A CN201710619051A CN107360969A CN 107360969 A CN107360969 A CN 107360969A CN 201710619051 A CN201710619051 A CN 201710619051A CN 107360969 A CN107360969 A CN 107360969A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention discloses a kind of method of the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province,Simultaneously,Culture medium suitable for the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province is provided,By the way that the capsule seed of the wild Dendrobium flexicaule of Mt. Funiushan In Henan Province is sowed in the wild Dendrobium flexicaule protocorm induction medium of Mt. Funiushan In Henan Province of targeted design formula,Pass through protocorm induction culturing seedling,The Dendrobium flexicaule seedling of Fiber differentiation is timely transferred on the wild Dendrobium flexicaule differentiation strong seedling culture base of Mt. Funiushan In Henan Province of targeted design formula,Directly it can go out with can descending to transplant the production seedling of domestication by fast breeding,Drastically increase tissue culture breeds efficiency and seedling quality,Survival rate significantly improves after kind transplantation of seedlings,The tissue-cultured seedling true leaf bred is plump,Root system is sturdy,Transplanting domestication is significantly improved to more than 98% by traditional 94%.
Description
Technical field
The present invention relates to Dendrobium flexicaule tissue-culturing rapid propagation seedling-raising technique field, and in particular to suitable for the wild song of Mt. Funiushan In Henan Province
The culture medium and method of stem dendrobe tissue culture rapid seedling cultivation.
Background technology
Dendrobium flexicaule（Scientific name：Dendrobium flexicaule Z. H. Tsi, S. C. Sun et L. G.
Xu）：Stem cylinder, slightly inflection sigmoid, branch, does not have several sections of, 2-4 pieces of leaf, and two arrange, and is mutually born in the top of stem, and nearly keratin is long
Round shape lanceolar.Inflorescence is sent from the old stem top for having fallen leaf, has 1-2 flower；Exhibition that the flowers are in blossom, petal bottom yellow green, top are near
Lavender, ellipse, is about 25 millimeters, and middle part is wide 13 millimeters, and tip is blunt, has 5 arteries and veins；Lip is faint yellow, and first end margin is pale purple
Color, middle part are split with lower edge purple, width egg shape, unobvious 3.May at florescence.Nodositas of the stem such as scorpion tail, the micro- green gold of color is yellow, often
Number plant, it is born at uninhabited and back of the body sun cliffs and precipices humidity, property slightly sweet flavor is slightly cold, and return lung, stomach, kidney channel, enriching yin are supported
Stomach, promote the production of body fluid to quench thirst, long term usage is promoted longevity, and widespread among the people has the function of coming back to life, and it is thirsty to cure mainly stomach-heat and dryness, consumptive disease win it is thin,
Wandering arthritis is palpitated with fear, and dysphoria, is had no peace of mind, and is preferably decocted long.
At present, the Dendrobium flexicaule in China is distributed mainly on Henan, Hubei, Hunan east, South Sichuan, the wild bent stem in Henan
In 800 on the moist precipitous cliff of 1200 meters of Funiu Shan Mountain virgin forest height above sea level, Nanyang area occupies the main growth distribution of the stem of noble dendrobium
More, because its growth conditions is very severe harsh, the effect of medicinal material is extremely obvious, and knot is drawn in clinical verification and medical experiment
By：The stem of noble dendrobium effect quality of all parts of the country is higher by, Henan Dendrobium flexicaule has very high fame and prestige in the industry of authentic medicinal herbs.Due to
Henan Dendrobium flexicaule is because its natural growing environment requires harsher, and wild Dendrobium flexicaule natural propagation ability is extremely low, and growth is slow
Slowly, yield poorly, the successive dynasties are all rare rare health-care good products, can only meet that few part population is enjoyed, and ordinary people is even all
Its unpleasant name.
In recent years, under the dint of hard work of countless scientific research personnel, the artificial tissue culture and planting technology of Dendrobium flexicaule obtain one
It is fixed to break through, but the experimental stage is mostly rested on, or excessive defect be present, it is not widely applied and is produced.《Stem of noble dendrobium tissue
Culture and cultivation technique research》In point out, embryo planting percent and embryo seedling using 14 kinds of culture mediums such as N6, MS to Dendrobium flexicaule
Growth is analyzed, and it is the result of optimal medium to have drawn N6 culture mediums.But N6 culture mediums are not suitable for Henan volt
The Seed embryo culture of the wild Dendrobium flexicaule of Ox Mountain, applicant have found in actual production, wild to Mt. Funiushan In Henan Province using N6 culture mediums
Phenomena such as raw Dendrobium flexicaule carries out tissue culture when breeding, and generally existing seedling is yellow, seedling is thin, root system is rare, and seedling development it is of poor quality,
Growth coefficient is low, and transplanting survival rate is low, it is also necessary to is improved according to the Biological characteristics of the wild Dendrobium flexicaule of Mt. Funiushan In Henan Province.
《Cultured in vitro and the regenerating system research of wild Dendrobium flexicaule》In point out, be using wild Dendrobium flexicaule stem section
Material is tried, has inquired into different growth regulators to its adventitious bud inducing, the influence bred and taken root, and establish wild Dendrobium flexicaule
High-efficiency regeneration system.By the use of Dendrobium flexicaule stem section as explant, plant fast propagation regenerating system is established, carries out tissue culture,
Although seedling can be obtained, breeding coefficient is too low, meanwhile, with the increase of subculture cycle, it may appear that seedling is degenerated, under quality
The generation of situations such as drop, vitrifying seedling re-establishes new regenerating system, time consuming, effect is failed to understand, it is necessary to change seedling
It is aobvious.
Applicant passes through substantial amounts of experimental study and comparative analysis, finds a kind of wild Dendrobium flexicaule tissue culture of Mt. Funiushan In Henan Province
The method of rapid seedling cultivation, according to the biological characteristics of the wild Dendrobium flexicaule of Mt. Funiushan In Henan Province, further effectively improve N6 bases
Culture medium prescription, the B family vitamin content and other additives for being advantageous to Dendrobium flexicaule growth are added, and mixed suitable
The hormone combination of Dendrobium flexicaule tissue-cultured seedling growth, the guarantor that this treasures rare plant variety to the wild Dendrobium flexicaule of Mt. Funiushan In Henan Province
A Tiao Xin roads are found in shield and exploitation.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province
Method, simultaneously, there is provided suitable for the culture medium of the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province, and solve to pass
Seedling quality is poor, growth coefficient is low, transplanting survival rate is low existing for type of rearing of uniting and seedling is degenerated, changes seedling, rebuilds again
The problem of raw body system.
In order to solve the above technical problems, the present invention is adopted the following technical scheme that to realize：
A kind of wild Dendrobium flexicaule protocorm induction medium of Mt. Funiushan In Henan Province, its prescription include consisting of point（Nothing is especially said
It is bright, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2750-2850, ammonium sulfate 462-466, potassium dihydrogen phosphate 395-405, magnesium sulfate 180-190,
Calcium chloride 160-168；
2nd, it is micro-：Manganese sulfate 4.0-4.8, zinc sulfate 1.2-1.8, boric acid 1.2-1.8, KI 0.75-0.85；
3rd, molysite：Ferrous sulfate 27.6-27.9, EDTA 37.2-37.4；
4th, organic matter：Inositol 98-104, sulfuric acid thiamine element 45-55, sulfuric acid pyridoxol 0.8-1.2, nicotinic acid 0.4-0.8, glycine
1.8-2.4；
5th, additive：Glycine betaine 490-510, Choline Chloride 290-320, sucrose 28-31g/L.
A kind of wild Dendrobium flexicaule differentiation strong seedling culture base of Mt. Funiushan In Henan Province, its prescription include consisting of point（Without especially
Illustrate, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2750-2850, ammonium sulfate 462-466, potassium dihydrogen phosphate 395-405, magnesium sulfate 180-190,
Calcium chloride 160-168；
2nd, it is micro-：Manganese sulfate 4.0-4.8, zinc sulfate 1.2-1.8, boric acid 1.2-1.8, KI 0.75-0.85；
3rd, molysite：Ferrous sulfate 27.6-27.9, EDTA 37.2-37.4；
4th, organic matter：Inositol 98-104, sulfuric acid thiamine element 45-55, sulfuric acid pyridoxol 0.8-1.2, nicotinic acid 0.4-0.8, glycine
1.8-2.4；
5th, hormone：6-BA 0.08-0.13、NAA 0.17-0.22；
6th, additive：Glycine betaine 490-510, Choline Chloride 290-320, sucrose 28-31g/L, agar powder 5.4-5.8g/L.
The pH value of above two culture medium controls：5.5—5.6.
A kind of method of the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province, comprises the following steps：
1）Dendrobium flexicaule protocorm Fiber differentiation seedling：The Dendrobium flexicaule capsule of maturation is taken to scrub dedusting in bromogeramine solution
Soil, it is placed on superclean bench, 10-20 min is sterilized with 0.2% mercuric chloride, then with aseptic water washing 3-5 time, by it with sterilizing
After filter paper suck dry moisture, cut at the top of fruit, then longitudinally splitted from middle part of fruit, seed is uniformly broadcasted sowing to the river prepared
In the southern wild Dendrobium flexicaule protocorm induction medium of Funiu Shan Mountain, the wild Dendrobium flexicaule protocorm of Mt. Funiushan In Henan Province after planting lures
Culture medium is led to be placed in 22 DEG C, intensity of illumination 1000-1500lux, cultivated under the daily light application time environmental condition of 10 hours
55-65 days；
2）Strong seedling culture：By step 1）The Dendrobium flexicaule seedling of Fiber differentiation is timely transferred on differentiation strong seedling culture base,
Under conditions of 24 DEG C of room temperature, intensity of illumination 1500-1800lux, daily illumination 12 hours, cultivate 90-100 days.
Strong seedling culture after 3 days it is visible transfer seedling start to grow, 7 days rear blades and stem mushroom out, and after 15 days, cane is bright
Aobvious to increase, 40-60 days, plant body progressively tended to ripe, after 90 days, each plant can long 4-6 pieces true leaf, 3-5 bar new roots, now
Tissue-cultured seedling can be used as production seedling under transplant domestication.
The application, which uses, sows the capsule seed of the wild Dendrobium flexicaule of Mt. Funiushan In Henan Province in the river of targeted design formula
In the southern wild Dendrobium flexicaule protocorm induction medium of Funiu Shan Mountain, by protocorm induction culturing seedling, it is noted that dilute during sowing
Broadcast, not broadcast too much, reserve the space for allowing seedling to grow, that broadcasts is too close, and seedling growth is excessively slim and frahile, is unfavorable for later stage training
Support.After induction culturing seedling, the Dendrobium flexicaule seedling of Fiber differentiation is timely transferred to the Henan of targeted design formula
On the wild Dendrobium flexicaule differentiation strong seedling culture base of Funiu Shan Mountain, you can direct fast breeding goes out with can descending to transplant the production seedling of domestication,
Drastically increase tissue culture breeds efficiency and seedling quality, and survival rate significantly improves after planting transplantation of seedlings, meanwhile, also avoid
The trouble that subculture seedling is degenerated, changes seedling, rebuilding regenerating system in existing mating system.
Choline Chloride in the application（Scientific name：2- ethoxys-trimethyl bursine）, one kind of vitamin B complex, it is one
The growth regulator of kind plant photosynthesis promoter, more wide spectrum.In general stem of noble dendrobium kind is different from due to Dendrobium flexicaule,
The epidermal cell of Dendrobium flexicaule blade is than relatively thin, and vascular bundle is more in leaf, and unit area stomata quantity is more.The net light of Dendrobium flexicaule
Conjunction rate, light saturation point, light compensation point are more general, and stem of noble dendrobium kind is all lower.Choline Chloride can be inhaled via the stem, leaf, root of plant
To receive, be then transmitted to the position worked faster, its physiological action promotes the photosynthesis of plant processed, promotes root system development,
It can make photosynthate is as much as possible to run up in stem tuber, root tuber and bulb.In addition, it is mixed with kinetin, class auxin,
Its movement can be accelerated, more effectively play the effect of kinetin, class auxin, reach the purpose for promoting plant growth.
Glycine betaine in the application culture medium is a kind of alkaloid, its molecular structure, application effect and natural glycine betaine without
Significant difference, belong to the natural goods equivalent of chemical synthesis（Scientific name：Betaine）.Glycine betaine effectively can maintain cell to ooze
Pressure thoroughly, avoid the murder by poisoning of cytoplasm high concentration inorganic ion-pair enzyme and metabolism；Lipid peroxidation metabolism degree is effectively reduced, is significantly reduced
Na water ratio in root system, by ca2+Root system is trapped in, the ability of root system resistance salt damage can be effectively improved, meanwhile, glycine betaine is to inverse
There is certain regulating and controlling effect under the conditions of border to stomatal movement, respiration and related gene expression.
Experiment confirms：The wild Dendrobium flexicaule protocorm induction medium Mt. Funiushan In Henan Province of Mt. Funiushan In Henan Province of the application is wild
The sprouting of Dendrobium flexicaule seed and seedling growth are respectively provided with good facilitation, the germination percentage of seed and the growth speed of embryoid
Degree and quality were all foreshortened to 55-60 days apparently higher than other conventional mediums, induction duration by traditional 60-70 days.Differentiation is strong
Seedling culture medium, by appropriate hormone, the fast-growth of seedling cauline leaf is greatly promoted, shortens 10-20 days compared with traditional approach,
The tissue-cultured seedling true leaf bred is plump, and root system is sturdy, and transplanting domestication is significantly improved to more than 98% by traditional 94%.
Embodiment
Embodiment one
The wild Dendrobium flexicaule protocorm induction medium of Mt. Funiushan In Henan Province of the present embodiment, its prescription include consisting of point（Nothing
Special instruction, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2800, ammonium sulfate 463, potassium dihydrogen phosphate 400, magnesium sulfate 185, calcium chloride 166；
2nd, it is micro-：Manganese sulfate 4.4, zinc sulfate 1.5, boric acid 1.6, KI 0.8；
3rd, molysite：Ferrous sulfate 27.8, EDTA 37.3；
4th, organic matter：Inositol 100, sulfuric acid thiamine element 50, sulfuric acid pyridoxol 1.0, nicotinic acid 0.5, glycine 2.0；
5th, additive：Glycine betaine 500, Choline Chloride 300, sucrose 30g/L.
The wild Dendrobium flexicaule differentiation strong seedling culture base of Mt. Funiushan In Henan Province of the present embodiment, its prescription include consisting of point
（Without special instruction, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2800, ammonium sulfate 463, potassium dihydrogen phosphate 400, magnesium sulfate 185, calcium chloride 166；
2nd, it is micro-：Manganese sulfate 4.4, zinc sulfate 1.5, boric acid 1.6, KI 0.8；
3rd, molysite：Ferrous sulfate 27.8, EDTA 37.3；
4th, organic matter：Inositol 100, sulfuric acid thiamine element 50, sulfuric acid pyridoxol 1.0, nicotinic acid 0.5, glycine 2.0；
5th, hormone：6-BA 0.1、NAA 0.2；
6th, additive：Glycine betaine 500, Choline Chloride 300, sucrose 30g/L, agar powder 5.6g/L.
The pH value of above two culture medium controls：5.5-5.6.
The method of the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province of the present embodiment, comprises the following steps：
1）Dendrobium flexicaule protocorm Fiber differentiation seedling：The Dendrobium flexicaule capsule of maturation is taken to scrub dedusting in bromogeramine solution
Soil, it is placed on superclean bench, 10-20 min is sterilized with 0.2% mercuric chloride, then with aseptic water washing 3-5 time, by it with sterilizing
After filter paper suck dry moisture, cut at the top of fruit, then longitudinally splitted from middle part of fruit, seed is uniformly broadcasted sowing to the river prepared
In the southern wild Dendrobium flexicaule protocorm induction medium of Funiu Shan Mountain, the wild Dendrobium flexicaule protocorm of Mt. Funiushan In Henan Province after planting lures
Culture medium is led to be placed in 22 DEG C, intensity of illumination 1300lux, carry out culture 60 days under the daily light application time environmental condition of 10 hours；
2）Strong seedling culture：By step 1）The Dendrobium flexicaule seedling of Fiber differentiation is timely transferred on differentiation strong seedling culture base,
Under conditions of 24 DEG C of room temperature, intensity of illumination 1600lux, daily illumination 12 hours, cultivate 95 days.Strong seedling culture is visible turn after 3 days
Move seedling to start to grow, 7 days rear blades and stem mushroom out, and after 15 days, cane substantially increases, 40-60 days, and plant body progressively becomes
To maturation, after 90 days, each plant can long 4-6 pieces true leaf, 3-5 bar new roots, now transplant domestication as under production seedling.
The Mt. Funiushan In Henan Province wild Dendrobium flexicaule breeding cycle of the present embodiment is 155 days, and the seedling of Fiber differentiation is more traditional
The seedling that culture medium is cultivated averagely weighs 1.2 times, and production seedling averagely weighs 1.4 times compared with the production seedling that conventional medium is cultivated, and transplanting is tamed and dociled
Change survival rate 99.1%.
Embodiment two
The present embodiment and the difference of embodiment one are：
The wild Dendrobium flexicaule protocorm induction medium of Mt. Funiushan In Henan Province, its prescription include consisting of point（Nothing is especially said
It is bright, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2750, ammonium sulfate 462, potassium dihydrogen phosphate 395, magnesium sulfate 180, calcium chloride 160；
2nd, it is micro-：Manganese sulfate 4.0, zinc sulfate 1.2, boric acid 1.2, KI 0.75；
3rd, molysite：Ferrous sulfate 27.6, EDTA 37.2；
4th, organic matter：Inositol 98, sulfuric acid thiamine element 45, sulfuric acid pyridoxol 0.8, nicotinic acid 0.4, glycine 1.8；
5th, additive：Glycine betaine 490, Choline Chloride 290, sucrose 28g/L.
The wild Dendrobium flexicaule differentiation strong seedling culture base of Mt. Funiushan In Henan Province, its prescription include consisting of point（Without especially
Illustrate, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2750, ammonium sulfate 462, potassium dihydrogen phosphate 395, magnesium sulfate 180, calcium chloride 160；
2nd, it is micro-：Manganese sulfate 4.0, zinc sulfate 1.2, boric acid 1.2, KI 0.75；
3rd, molysite：Ferrous sulfate 27.6, EDTA 37.2；
4th, organic matter：Inositol 98, sulfuric acid thiamine element 45, sulfuric acid pyridoxol 0.8, nicotinic acid 0.4, glycine 1.8；
5th, hormone：6-BA 0.08、NAA 0.17；
6th, additive：Glycine betaine 490, Choline Chloride 290, sucrose 28g/L, agar powder 5.4g/L.
In the method for the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province, step 1）Middle intensity of illumination
1000lux, culture 65 days；Step 2）Intensity of illumination 1500lux, cultivate 100 days.
The Mt. Funiushan In Henan Province wild Dendrobium flexicaule breeding cycle of the present embodiment is 165 days, and the seedling of Fiber differentiation is more traditional
The seedling that culture medium is cultivated averagely weighs 1.1 times, and production seedling averagely weighs 1.4 times compared with the production seedling that conventional medium is cultivated, and transplanting is tamed and dociled
Change survival rate 98.4%.
Embodiment three
The present embodiment and the difference of embodiment one are：
The wild Dendrobium flexicaule protocorm induction medium of Mt. Funiushan In Henan Province, its prescription include consisting of point（Nothing is especially said
It is bright, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2850, ammonium sulfate 466, potassium dihydrogen phosphate 405, magnesium sulfate 190, calcium chloride 168；
2nd, it is micro-：Manganese sulfate 4.8, zinc sulfate 1.8, boric acid 1.8, KI 0.85；
3rd, molysite：Ferrous sulfate 27.9, EDTA 37.4；
4th, organic matter：Inositol 104, sulfuric acid thiamine element 55, sulfuric acid pyridoxol 1.2, nicotinic acid 0.8, glycine 2.4；
5th, additive：Glycine betaine 510, Choline Chloride 320, sucrose 31g/L.
The wild Dendrobium flexicaule differentiation strong seedling culture base of Mt. Funiushan In Henan Province, its prescription include consisting of point（Without especially
Illustrate, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2850, ammonium sulfate 466, potassium dihydrogen phosphate 405, magnesium sulfate 190, calcium chloride 168；
2nd, it is micro-：Manganese sulfate 4.8, zinc sulfate 1.8, boric acid 1.8, KI 0.85；
3rd, molysite：Ferrous sulfate 27.9, EDTA 37.4；
4th, organic matter：Inositol 104, sulfuric acid thiamine element 55, sulfuric acid pyridoxol 1.2, nicotinic acid 0.8, glycine 2.4；
5th, hormone：6-BA 0.13、NAA 0.22；
6th, additive：Glycine betaine 510, Choline Chloride 320, sucrose 31g/L, agar powder 5.8g/L.
In the method for the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province, step 1）Middle intensity of illumination
1500lux, culture 55 days；Step 2）Intensity of illumination 1800lux, cultivate 90 days.
The Mt. Funiushan In Henan Province wild Dendrobium flexicaule breeding cycle of the present embodiment is 145 days, and the seedling of Fiber differentiation is more traditional
The seedling that culture medium is cultivated averagely weighs 1.2 times, and production seedling averagely weighs 1.3 times compared with the production seedling that conventional medium is cultivated, and transplanting is tamed and dociled
Change survival rate 98.2%.
Example IV
The present embodiment and the difference of embodiment one are：
The wild Dendrobium flexicaule protocorm induction medium of Mt. Funiushan In Henan Province, its prescription include consisting of point（Nothing is especially said
It is bright, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2800, ammonium sulfate 465, potassium dihydrogen phosphate 400, magnesium sulfate 188, calcium chloride 165；
2nd, it is micro-：Manganese sulfate 4.6, zinc sulfate 1.7, boric acid 1.4, KI 0.82；
3rd, molysite：Ferrous sulfate 27.8, EDTA 37.3；
4th, organic matter：Inositol 100, sulfuric acid thiamine element 50, sulfuric acid pyridoxol 1.0, nicotinic acid 0.6, glycine 2.0；
5th, additive：Glycine betaine 505, Choline Chloride 310, sucrose 30g/L.
The wild Dendrobium flexicaule differentiation strong seedling culture base of Mt. Funiushan In Henan Province, its prescription include consisting of point（Without especially
Illustrate, unit mg/L）：
1st, a great number of elements：Potassium nitrate 2840, ammonium sulfate 463, potassium dihydrogen phosphate 400, magnesium sulfate 188, calcium chloride 166；
2nd, it is micro-：Manganese sulfate 4.5, zinc sulfate 1.6, boric acid 1.6, KI 0.8；
3rd, molysite：Ferrous sulfate 27.7, EDTA 37.3；
4th, organic matter：Inositol 102, sulfuric acid thiamine element 48, sulfuric acid pyridoxol 1.1, nicotinic acid 0.6, glycine 2.1；
5th, hormone：6-BA 0.11、NAA 0.19；
6th, additive：Glycine betaine 500, Choline Chloride 310, sucrose 29g/L, agar powder 5.6g/L.
In the method for the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province, step 1）Middle intensity of illumination
1400lux, culture 60 days；Step 2）Intensity of illumination 1600lux, cultivate 95 days.
The Mt. Funiushan In Henan Province wild Dendrobium flexicaule breeding cycle of the present embodiment is 155 days, and the seedling of Fiber differentiation is more traditional
The seedling that culture medium is cultivated averagely weighs 1.3 times, and production seedling averagely weighs 1.5 times compared with the production seedling that conventional medium is cultivated, and transplanting is tamed and dociled
Change survival rate 98.8%.
The Mt. Funiushan In Henan Province of the present invention is short wild Dendrobium flexicaule breeding cycle, and seedling quality is high, robust plant, transplants lowerly
The survival rate of domestication is generally in 98.2-99%.
Claims (4)
- A kind of 1. wild Dendrobium flexicaule protocorm induction medium of Mt. Funiushan In Henan Province, it is characterised in that：Its prescription is included with the following group Composition（Without special instruction, unit mg/L）：1）A great number of elements：Potassium nitrate 2750-2850, ammonium sulfate 462-466, potassium dihydrogen phosphate 395-405, magnesium sulfate 180-190, Calcium chloride 160-168；2）Trace element：Manganese sulfate 4.0-4.8, zinc sulfate 1.2-1.8, boric acid 1.2-1.8, KI 0.75-0.85；3）Molysite：Ferrous sulfate 27.6-27.9, EDTA 37.2-37.4；4）Organic matter：Inositol 98-104, sulfuric acid thiamine element 45-55, sulfuric acid pyridoxol 0.8-1.2, nicotinic acid 0.4-0.8, glycine 1.8-2.4；5）Additive：Glycine betaine 490-510, Choline Chloride 290-320, sucrose 28-31g/L.
- A kind of 2. wild Dendrobium flexicaule differentiation strong seedling culture base of Mt. Funiushan In Henan Province, it is characterised in that：Its prescription includes consisting of Point（Without special instruction, unit mg/L）：1）A great number of elements：Potassium nitrate 2750-2850, ammonium sulfate 462-466, potassium dihydrogen phosphate 395-405, magnesium sulfate 180-190, Calcium chloride 160-168；2）Trace element：Manganese sulfate 4.0-4.8, zinc sulfate 1.2-1.8, boric acid 1.2-1.8, KI 0.75-0.85；3）Molysite：Ferrous sulfate 27.6-27.9, EDTA 37.2-37.4；4）Organic matter：Inositol 98-104, sulfuric acid thiamine element 45-55, sulfuric acid pyridoxol 0.8-1.2, nicotinic acid 0.4-0.8, glycine 1.8-2.4；5）Hormone：6-BA 0.08-0.13、NAA 0.17-0.22；6）Additive：Glycine betaine 490-510, Choline Chloride 290-320, sucrose 28-31g/L, agar powder 5.4-5.8g/L.
- 3. culture medium as claimed in claim 1 or 2, it is characterised in that its pH value controls：5.5—5.6.
- A kind of 4. method of the wild Dendrobium flexicaule tissue-culturing rapid propagation nursery of Mt. Funiushan In Henan Province, it is characterised in that comprise the following steps：1）Dendrobium flexicaule protocorm Fiber differentiation seedling：The Dendrobium flexicaule capsule of maturation is taken to scrub dedusting in bromogeramine solution Soil, it is placed on superclean bench, 10-20 min is sterilized with 0.2% mercuric chloride, then with aseptic water washing 3-5 time, by it with sterilizing After filter paper suck dry moisture, cut at the top of fruit, then longitudinally splitted from middle part of fruit, seed is uniformly broadcasted sowing to the river prepared In the southern wild Dendrobium flexicaule protocorm induction medium of Funiu Shan Mountain, the wild Dendrobium flexicaule protocorm of Mt. Funiushan In Henan Province after planting lures Culture medium is led to be placed in 22 DEG C, intensity of illumination 1000-1500lux, cultivated under the daily light application time environmental condition of 10 hours 55-65 days；2）Strong seedling culture：By step 1）The Dendrobium flexicaule seedling of Fiber differentiation is timely transferred on differentiation strong seedling culture base, Under conditions of 24 DEG C of room temperature, intensity of illumination 1500-1800lux, daily illumination 12 hours, cultivate 90-100 days.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108522218A (en) * | 2018-05-11 | 2018-09-14 | 安徽霍山县草之灵中药材有限公司 | A kind of cultivation matrix improving dendrobium candidum sprouting upgrowth situation |
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| CN115136891A (en) * | 2022-06-28 | 2022-10-04 | 广东金颖花卉苗木有限公司 | Method for inducing dendrobium nobile pseudoprotocorm |
| CN115176688A (en) * | 2022-07-12 | 2022-10-14 | 神农架国家公园科学研究院 | Wild-imitating planting method for dendrobium kojima |
| CN115176688B (en) * | 2022-07-12 | 2023-08-11 | 神农架国家公园科学研究院 | Wild-imitating planting method for dendrobium candidum |
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