CN115039796A - Acaricidal bacteriostatic agent containing plant essential oil and preparation method and application thereof - Google Patents

Acaricidal bacteriostatic agent containing plant essential oil and preparation method and application thereof Download PDF

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CN115039796A
CN115039796A CN202210528717.8A CN202210528717A CN115039796A CN 115039796 A CN115039796 A CN 115039796A CN 202210528717 A CN202210528717 A CN 202210528717A CN 115039796 A CN115039796 A CN 115039796A
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essential oil
mite
bacteriostatic agent
killing
oil
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李媛媛
赵金红
周鸿铭
滕俏
薛琪琪
邹茗惠
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Wannan Medical College
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A—HUMAN NECESSITIES
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    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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    • A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
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    • A—HUMAN NECESSITIES
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    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P17/00—Pest repellants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/02—Acaricides

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Abstract

The invention discloses a mite-killing bacteriostatic agent containing plant essential oil, which specifically comprises the following components in parts by mass: 0.02-0.04% of rosemary essential oil, 0.04-0.08% of eucalyptus essential oil, 0.01-0.03% of galangal essential oil, 0.01-0.03% of patchouli essential oil, 2-5% of propylene glycol, 2-5% of laurylamine dipropylene diamine, 1-2% of PEG-40 hydrogenated castor oil and 80-90% of deionized water. Adding the prepared essential oil and various raw materials of the solvent into a stirring reaction container one by one, wherein the rotating speed is 200-300rpm, and the stirring time is 25-30 minutes, so as to prepare the composite essential oil. The invention takes PEG-40 hydrogenated castor oil as an emulsifier, and uses laurylamine-diylidene-dipropylamine as a solubilizer to promote the fusion of essential oil and water, so that oily substances such as essential oil and the like are uniformly dispersed in the water to form a more stable and transparent solution system, and the patchouli essential oil and the galangal essential oil are creatively added to prepare the acaricidal bacteriostatic agent, thereby having more efficient killing effect on mites.

Description

Acaricidal bacteriostatic agent containing plant essential oil and preparation method and application thereof
Technical Field
The invention relates to the technical field of cleaning products, in particular to a mite-killing bacteriostatic agent containing plant essential oil and a preparation method thereof, which are mainly used for household environment products such as mite-killing spray, pet cleaning products, air conditioner bactericide and the like, and have mite-killing and bacteriostatic effects.
Background
Dust mites belong to a class of microscopically small macroscopic arthropods of the subclass Acarina, the phylum Arthropoda. Dust mites are everywhere in daily life, and a large number of dust mites exist in bedding mattresses, wardrobe clothing, shoe cabinets, children toys, carpet sofas and all corners of rooms. The dust mites are tiny in body type but harmful, and can cause allergic eczema/dermatitis, urticaria, allergic rhinitis and the like besides asthma, so that the health of a human body is seriously harmed, and the problem of dust mite allergy is increasingly valued by people.
At present, common chemical acaricides (benzyl benzoate, tannic acid and the like) on the market have good control effect on dust mites, but have certain influence on human health, have the defects of harming non-target organisms, polluting the environment and the like, and are not suitable for being applied indoors. Therefore, the research for finding safe, efficient and pollution-free plant source acaricide to replace chemical acaricide is becoming a hot spot.
At present, plant essential oil is widely applied to the fields of spices, medicines, foods and the like. The essential oil has various action modes such as fumigating, contact killing, luring, repelling and the like on pests, and some essential oil is also rich in ideal acaricidal active substances and shows good biological activity on various mites. The plant essential oil is an acaricide which is more green, environment-friendly, safe and efficient than the traditional chemical agents, is friendly to the environment and human health, and can be used for a long time. However, as can be seen from the analysis of the existing plant essential oil, the oily components such as essential oil are difficult to be fused with the solvent, so that the oily components are difficult to be uniformly distributed in the preparation and use processes, and the properties are difficult to be stable, thereby affecting the final mite-killing and bacteriostatic effects.
Disclosure of Invention
The invention aims to provide a miticidal bacteriostatic agent containing plant essential oil, and a preparation method and application thereof, so as to solve the technical problems of uneven distribution and poor stability of an essential oil system in the prior art.
In order to solve the technical problems, the invention specifically provides the following technical scheme:
a mite-killing bacteriostatic agent containing plant essential oil comprises the following components in parts by mass:
0.02-0.04% of rosemary essential oil, 0.04-0.08% of eucalyptus essential oil, 0.01-0.03% of galangal essential oil, 0.01-0.03% of patchouli essential oil, 2-5% of propylene glycol, 2-5% of laurylamine dipropylene diamine, 1-2% of PEG-40 hydrogenated castor oil and 80-90% of deionized water.
In order to solve the above technical problems, the present invention further provides the following technical solutions:
the preparation method based on the acaricidal bacteriostatic agent comprises the following steps:
100, putting propylene glycol into a mixing vessel, adding laurylamine dipropylene diamine and PEG-40 hydrogenated castor oil according to a mass ratio, and performing magnetic stirring and ultrasonic treatment for 20-30min by using an ultrasonic oscillator after primary mixing to obtain an emulsification solubilization system;
200, putting rosemary essential oil, eucalyptus essential oil, patchouli essential oil and galangal essential oil into a stirring vessel in sequence, and performing magnetic stirring and ultrasonic treatment for 20-30min by using an ultrasonic oscillator to obtain a compound essential oil system;
step 300, adding the compound essential oil system in the step 200 into the emulsification solubilization system in the step 100, adding deionized water for multiple times according to the mass ratio, and performing magnetic stirring and ultrasonic treatment for 30-40min by an ultrasonic oscillator to obtain the acaricidal bacteriostatic agent.
Further, the magnetic stirring and ultrasonic oscillator ultrasonic treatment both adopt a repeated cycle staggered operation, specifically:
each cycle period is 5min, wherein the magnetic stirring is carried out for 3min, and the ultrasonic oscillator is used for ultrasonic treatment for 2 min.
Further, between each cycle period, froth is removed by a demister.
Further, in step 200, rosemary essential oil, eucalyptus essential oil, patchouli essential oil and galangal essential oil are put into a stirring vessel in sequence, and deionized water is added for preliminary mixing between each time;
and adding a magnetic composite material as a carrier into the mixture of the essential oil, and carrying out magnetic stirring and ultrasonic treatment by an ultrasonic oscillator to obtain a semi-emulsified compound essential oil system.
Furthermore, the magnetic composite materials are insoluble in water and have different shapes, the particle size of each magnetic composite material is 0.05-0.6 mm, and a hole or a throat is formed in each magnetic composite material.
Furthermore, the invention also provides application of the acaricidal and bacteriostatic agent in preparation of daily necessities.
Wherein:
the daily necessities comprise laundry soap, mite-removing laundry detergent, mite-removing bags, mite-removing spray, pet detergent, air-conditioner bactericide, washing machine cleaning agent and environment cleaning agent.
Compared with the prior art, the invention has the following beneficial effects:
the invention takes PEG-40 hydrogenated castor oil as an emulsifier, and uses laurylamine diylidene dipropylamine as a solubilizer to promote the fusion of essential oil and water, so that oily substances such as the essential oil and the like are uniformly dispersed in the water to form a more stable and transparent solution system, wherein, on the basis of the conventional rosemary essential oil and the eucalyptus essential oil, the acaricidal bacteriostatic agent prepared by adding the patchouli essential oil and the galangal essential oil is creatively used, thereby having more efficient killing effect on acarids, and the whole system is non-toxic and harmless and has no side effect on environment and human body.
The invention is based on the formula and combines the magnetic stirring and the ultrasonic treatment of the ultrasonic oscillator to better mix the components, thereby improving the uniformity of the product.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
Fig. 1 is a schematic flow chart provided in an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in figure 1, the invention provides a miticidal bacteriostatic agent containing plant essential oil, a preparation method and application thereof.
Wherein:
the active ingredients of the mite-killing bacteriostatic agent are mainly a composition of rosemary essential oil, eucalyptus leaf essential oil, galangal essential oil and patchouli essential oil.
The acaricidal bacteriostatic agent containing the plant essential oil comprises an essential oil part and a solvent part, wherein the essential oil part comprises the following components in percentage by mass:
Figure BDA0003645677220000041
the solvent part comprises the following components in percentage by mass according to the total content:
80 to 90 percent of water
2 to 5 percent of propylene glycol
2-5% of laurylamine dipropylene diamine
PEG-40 hydrogenated Castor oil 1-2%
In the embodiment of the invention, the compound essential oil is compounded by natural essential oil, is non-toxic and harmless, has aromatic smell and is environment-friendly, and the higher mite removing effect is realized under the condition of very low addition of the essential oil by accurately adjusting the proportion of various essential oils, so that the compound essential oil can be used for spraying, cleaning agents, household bedding articles, pet articles and the like.
Wherein, the essential oil adopted in the invention has the following functions:
rosemary essential oil:
rosemary, also known as blumea, belongs to Rosmarinus of Labiatae, and is a perennial evergreen shrub which prefers warm climate. The flower and leaf can be extracted to obtain rosemary essential oil, has fragrant smell, and has effects of refreshing brain, tranquilizing mind, relieving and improving memory. The research shows that the rosemary essential oil has multiple effects of oxidation resistance, bacteriostasis, inflammation resistance, tumor resistance, insect killing and the like. Researchers have explored the inhibiting and killing effect of rosemary oil on tetranychus cinnabarinus by a contact killing mortality bioassay method, and explored the inhibiting and killing effect mechanism of rosemary oil on tetranychus cinnabarinus by measuring the activity of three protective enzymes, namely superoxide dismutase (SOD), Peroxidase (POD) and Catalase (CAT), in mites.
The rosemary essential oil in the compound essential oil provided by the embodiment of the invention can play a role in removing mites and also has an antibacterial effect.
Eucalyptus leaf essential oil:
eucalyptus is a evergreen tree of the genus eucalyptus of the family myrtaceae, and a large number of volatile chemical components, mainly terpenes (including monoterpenes, sesquiterpenes, etc.), aliphatic compounds, aromatic compounds and other compounds, have been found in plants of the genus eucalyptus so far. Modern pharmacological research has proved that the chemical components of the eucalyptus essential oil have the effects of resisting bacteria, diminishing inflammation, improving respiratory function, killing insects, resisting oxidation, resisting inflammation, relieving pain and the like.
The eucalyptus leaf essential oil in the composite essential oil provided by the embodiment of the invention can play a role in removing mites and also has an antibacterial effect.
Galangal essential oil:
rhizoma Alpiniae Officinarum refers to rhizome of Alpinia Officinarum of Alpinia of Zingiberaceae, and has effects of warming stomach, relieving vomit, eliminating dampness, and activating spleen. Modern researches show that the main chemical components of the traditional Chinese medicine composition comprise various chemical components such as volatile oil, flavonoid, diphenyl heptane, polysaccharide and the like, and the traditional Chinese medicine composition has the effects of resisting ulcer, tumors, inflammation, pain and bacteria, and the volatile oil is one of the main effective components. Meanwhile, galangal is one of medicinal and edible medicinal materials identified by Ministry of health.
The galangal volatile oil has a relatively obvious inhibition effect on the growth of yeasts, gram-positive bacteria and gram-negative bacteria, and has an inhibition zone of 9-15 mm on staphylococcus aureus, escherichia coli, bacillus subtilis and saccharomyces cerevisiae.
The alpinia galanga essential oil in the composite essential oil provided by the embodiment of the invention can play a role in removing mites and also has an antibacterial effect.
Patchouli essential oil:
pogostemon cablin is an important clinical traditional Chinese medicine in China, and has the effects of eliminating turbid pathogen with aromatics, stimulating the appetite, relieving vomiting, relieving exterior syndrome, relieving summer heat and the like. The volatile oil is also one of the main effective components of patchouli, wherein patchouli alcohol and patchoulenone are the main antibacterial effective components.
The patchouli essential oil in the compound essential oil provided by the embodiment of the invention can play a role in removing mites and also has an antibacterial effect.
According to the above, the rosemary essential oil, the eucalyptus leaf essential oil, the galangal essential oil and the patchouli essential oil in the plant essential oil have good mite removing and bacteriostasis effects, and the searching of mite removing active ingredients which are stable in property, safe, efficient, low in toxicity and suitable for household articles and environment from the plant essential oil is the primary target of product development. In addition, the galangal essential oil and the patchouli essential oil related in the embodiment of the invention are not disclosed to be applied to the field of mite and bacteria inhibition in the prior art.
In addition, the solvent in the embodiment of the invention adopts laurylamine dipropylenediamine as a solubilizer, so that the mixing of water and essential oil can be promoted, and the solvent has the properties of emulsification, lubrication, solubilization, antibiosis, sterilization and corrosion resistance; propylene glycol is adopted as a solvent and a volatilization promoter; the PEG-40 hydrogenated castor oil is used as an emulsifier and can promote the fusion of essential oil and water; the solvent in the embodiment of the invention can keep the optimal solubility and stability of the essential oil.
The components of the composite plant essential oil acaricide disclosed by the invention supplement each other to release special fragrance, so that the effect of efficiently expelling mites is achieved, and the acaricide is non-toxic and harmless and has no side effect on the environment and human bodies.
First, testing process
Example 1
0.04% of rosemary essential oil, 0.06% of eucalyptus leaf essential oil, 0.02% of galangal essential oil and 0.02% of patchouli essential oil.
5% propylene glycol, 3% laurylamine dipropylene diamine, 1% PEG-40 hydrogenated castor oil, and the balance water.
Example 2
0.03% of rosemary essential oil, 0.07% of eucalyptus leaf essential oil, 0.02% of galangal essential oil and 0.02% of patchouli essential oil.
Example 3
0.03% of rosemary essential oil, 0.07% of eucalyptus leaf essential oil, 0.03% of galangal essential oil and 0.01% of patchouli essential oil.
Example 4
0.02% of rosemary essential oil, 0.08% of eucalyptus leaf essential oil, 0.02% of galangal essential oil and 0.02% of patchouli essential oil.
Comparative example 1
Essentially the same as example 1 except that no patchouli essential oil was added; 0.04% of rosemary essential oil, 0.06% of eucalyptus leaf essential oil and 0.02% of galangal essential oil.
Comparative example 2
The same as example 1 except that no galangal oil was added; 0.04% of rosemary essential oil, 0.06% of eucalyptus leaf essential oil and 0.02% of patchouli essential oil.
Comparative example 3
Essentially the same as example 1 except that no eucalyptus essential oil was added; 0.04% of rosemary essential oil, 0.02% of galangal essential oil and 0.02% of patchouli essential oil.
Comparative example 4
Essentially the same as example 1 except that rosemary essential oil was not added; 0.06% of eucalyptus leaf essential oil, 0.02% of galangal rhizome essential oil and 0.02% of patchouli essential oil.
For the above examples and comparative examples, the prepared essential oil and various raw materials of the solvent are added into the stirring reaction vessel one by one, the rotation speed is 200-300rpm, and the stirring time is 25-30 minutes, so as to prepare the composite essential oil.
For the preparation method, the specific mode is as follows:
a preparation method of the acaricidal bacteriostatic agent based on the claim 1, which comprises the following steps:
100, putting propylene glycol into a mixing vessel, adding laurylamine dipropylene diamine and PEG-40 hydrogenated castor oil according to a mass ratio, and performing magnetic stirring and ultrasonic treatment for 20-30min by using an ultrasonic oscillator after primary mixing to obtain an emulsification solubilization system;
200, putting rosemary essential oil, eucalyptus essential oil, patchouli essential oil and galangal essential oil into a stirring vessel in sequence, and performing magnetic stirring and ultrasonic treatment for 20-30min by an ultrasonic oscillator to obtain a compound essential oil system;
step 300, adding the compound essential oil system in the step 200 into the emulsification solubilization system in the step 100, adding deionized water for multiple times according to the mass ratio, and performing magnetic stirring and ultrasonic treatment for 30-40min by an ultrasonic oscillator to obtain the acaricidal bacteriostatic agent.
The magnetic stirring and ultrasonic oscillator ultrasonic treatment adopt repeated cycle staggered operation, and specifically comprise the following steps: each cycle period is 5min, wherein the magnetic stirring is carried out for 3min, and the ultrasonic oscillator is used for ultrasonic treatment for 2 min.
All remove the foam through the demister between every circulation cycle, can eliminate some foam through the effect of demister, can improve the mixed effect, in addition, can also be through foam and the elimination of demister can further improvement mixed effect for each composition can distribute evenly.
Further, in step 200, rosemary essential oil, eucalyptus essential oil, patchouli essential oil and galangal essential oil are put into a stirring vessel in sequence, and deionized water is added for preliminary mixing between each time;
and adding a magnetic composite material as a carrier into the mixture of the essential oil, and performing magnetic stirring and ultrasonic treatment by an ultrasonic oscillator to obtain a semi-emulsified compound essential oil system.
The materials are added in sequence, the aim of mixing can be achieved in a plurality of times through the action of deionized water, and the final integral mixing effect is improved through a plurality of times of addition.
Furthermore, the magnetic composite materials are insoluble in water and have different shapes, the particle size of each magnetic composite material is 0.05-0.6 mm, and a hole or a throat is formed in each magnetic composite material. Through the disturbance of the magnetic composite material under the action of magnetic force, the mixing efficiency and the mixing degree of all the components can be improved.
Second, test results
(1) The complex essential oils of examples 1 to 4 and the complex essential oils of comparative examples 1 to 4 were subjected to mite extermination test.
The mite species were tested: dust mites, male and female adult mites, nymphs;
the test method comprises the following steps: refer to NY/T1151.2-2006 pesticide laboratory test methods for pesticide registration sanitation and evaluation section 2: the acaricidal agent and the acaricidal agent are labeled as a test method, and the test results are shown in table 1.
TABLE 1 Deacaricidal test results
Sample(s) Test mite number (only) Number of dead mites (only) Acarid eradication rate (%) Corrected mite-killing rate (%)
Example 1 226 226 100 100
Example 2 219 219 100 100
Example 3 223 223 100 100
Example 4 221 221 100 100
Comparative example 1 227 227 100 100
Comparative example 2 230 177 76.92 75.23
Comparative example 3 227 147 64.75 62.17
Comparative example 4 217 195 89.86 88.99
Blank control 120 8 6.81 --------
Note: correcting mite-killing rate (experimental mite-killing rate of control group)/(1-mite-killing rate of control group)
(2) The composite essential oils of examples 1-4 and the composite essential oils of comparative examples 1-4 are subjected to sterilization tests according to the evaluation method of antibacterial and bacteriostatic effects of QB/T2738-.
TABLE 2 inhibition results of the three bacteria by the complex essential oils
Figure BDA0003645677220000081
Figure BDA0003645677220000091
In summary, the above analysis of the test data shows that: the mite killing and bacteriostasis effects of the compound essential oil prepared in the comparative examples 1-4 are obviously lower than those of the compound essential oil prepared in the examples 1-4.
According to the combination of the foregoing examples and comparative examples, the experimental results of examples 1-4 and comparative examples 1-4 show that the effects of killing and inhibiting bacteria of four kinds of mites are improved when the mites are mixed together, and the effects are not as good as those after mixing when a single essential oil experiment is performed. Moreover, importantly, the compound essential oil is not reported to be used in mite-killing and bacteria-removing experiments, in particular to galangal essential oil and patchouli essential oil.
In addition, the invention selects the laurylamine-diylidene dipropylamine as the solubilizer instead of the mite-killing and sterilizing active substance disclosed in the prior art, has better effect compared with other solubilizers, and can ensure that the plant essential oil supplements each other to release special fragrance, thereby achieving the effect of more efficiently killing and expelling mites.
Example 5
This example examines the effect of the compound essential oil formulations of examples 1-4 in the use of bacteriostatic detergent products.
The antibacterial activity test is carried out by referring to QB/T2850-2007 antibacterial and bacteriostatic detergent, the content of the compound essential oil is 3 percent in examples 1-4, the corresponding relations are example 1-A, example 2-B, example 3-C and example 4-D, and corresponding letters are used as sample numbers. During the test, the detergent is diluted according to the proportion of 1:100, and the bacteriostatic effect of the detergent is examined.
Test samples: mite-killing and bacteria-inhibiting detergent was prepared according to Table 3
TABLE 3 preparation of mite-killing and bacteriostatic detergent
Figure BDA0003645677220000092
Figure BDA0003645677220000101
The test results are shown in table 4:
TABLE 4 inhibitory Effect of the mite-killing bacteriostatic detergent on three bacteria
Sample (I) Escherichia coli Candida albicans Staphylococcus aureus
A >99.97％ >99.97％ >99.97％
B >99.97％ >99.97％ >99.97％
C >99.97％ >99.97％ >99.97％
D >99.97％ >99.97％ >99.97％
The test result shows that: the compound essential oil has excellent bacteriostatic effect in practical application, the smell is fresh (self smell) when the plant essential oil is added, and the compound essential oil can be used as an excellent bacteriostatic agent for household daily chemical products.
Example 6
This example examines the effect of the compound essential oils of examples 1-4 in several mite-killing products
Refer to NY/T1151.2-2006 pesticide laboratory test methods for pesticide registration sanitation and evaluation section 2: the label test method of mite killing and expelling agent.
1. Test samples were prepared according to tables 5, 6 and 7.
TABLE 5 preparation of mite-killing spray
Composition (A) Content (wt.)
Acrylic lanolin 20％
Sodium cocoate 10％
Sorbitan 3％
Salicylic acid 2％
Compound essential oil (example 1) 2％
Deionized water To 100%
TABLE 6 formulation of mite-killing shampoo for pets
Figure BDA0003645677220000102
Figure BDA0003645677220000111
TABLE 7 formulation of air conditioner cleaners
Composition (I) Content (wt.)
Sodium dodecyl benzene sulfonate 2％
Propylene carbonate 1.5％
Dipropylene glycol monobutyl ether 2.5％
OP-10 0.5％
Triethanolamine 0.2％
Compound essential oil (example 3) 1.5％
Deionized water To 100 percent
2. The mite species were tested: dust mite, adult female and male, and nymph.
3. And (3) testing results: see Table 8
TABLE 8 Deacaricidal test results for several of the Deacaricidal products used
Sample (I) Test mite number (only) Number of dead mites (only) Mite eradication Rate (%) Corrected mite-killing rate (%)
Mite-killing spray 231 231 100 100
Mite-removing shampoo for pets 225 225 100 100
Air conditioner cleaning agent 228 228 100 100
Blank control 221 9 4.10 --------
As can be seen from Table 8, several mite killing products containing the compound essential oil show excellent mite killing effect in various furniture environments and pet products, and have high practical application value.
The synthesis of the above steps:
the invention takes PEG-40 hydrogenated castor oil as an emulsifier, and uses laurylamine diylidene dipropylamine as a solubilizer to promote the fusion of essential oil and water, so that oily substances such as the essential oil and the like are uniformly dispersed in the water to form a more stable and transparent solution system, wherein, on the basis of the conventional rosemary essential oil and the eucalyptus essential oil, the acaricidal bacteriostatic agent prepared by adding the patchouli essential oil and the galangal essential oil is creatively used, thereby having more efficient killing effect on acarids, and the whole system is non-toxic and harmless and has no side effect on environment and human body.
The above embodiments are only exemplary embodiments of the present application, and are not intended to limit the present application, and the protection scope of the present application is defined by the claims. Various modifications and equivalents may be made by those skilled in the art within the spirit and scope of the present application and such modifications and equivalents should also be considered to be within the scope of the present application.

Claims (8)

1. The acaricidal bacteriostatic agent containing plant essential oil is characterized by comprising the following components in percentage by mass:
0.02-0.04% of rosemary essential oil, 0.04-0.08% of eucalyptus leaf essential oil, 0.01-0.03% of galangal essential oil, 0.01-0.03% of patchouli essential oil, 2-5% of propylene glycol, 2-5% of laurylamine dipropylene diamine, 1-2% of PEG-40 hydrogenated castor oil and 80-90% of deionized water.
2. The preparation method of the acaricidal bacteriostatic agent based on the claim 1 is characterized by comprising the following steps:
100, putting propylene glycol into a mixing vessel, adding laurylamine dipropylene diamine and PEG-40 hydrogenated castor oil according to a mass ratio, and performing magnetic stirring and ultrasonic treatment for 20-30min by using an ultrasonic oscillator after primary mixing to obtain an emulsification solubilization system;
200, putting rosemary essential oil, eucalyptus essential oil, patchouli essential oil and galangal essential oil into a stirring vessel in sequence, and performing magnetic stirring and ultrasonic treatment for 20-30min by an ultrasonic oscillator to obtain a compound essential oil system;
step 300, adding the compound essential oil system in the step 200 into the emulsification solubilization system in the step 100, adding deionized water for multiple times according to the mass ratio, and performing magnetic stirring and ultrasonic treatment for 30-40min by an ultrasonic oscillator to obtain the acaricidal bacteriostatic agent.
3. The preparation method of the acaricide bacteriostat according to claim 2, wherein the magnetic stirring and ultrasonic oscillator ultrasonic treatment are both operated in a repeated cycle staggered mode, and specifically comprises the following steps:
each cycle period is 5min, wherein the magnetic stirring is carried out for 3min, and the ultrasonic oscillator is used for ultrasonic treatment for 2 min.
4. The method for preparing a miticidal and bacteriostatic agent according to claim 3, wherein the defoaming is carried out by a demister between each cycle.
5. The method for preparing the acaricide bacteriostat according to claim 2, wherein in step 200, rosemary essential oil, eucalyptus essential oil, patchouli essential oil and galangal essential oil are put into a stirring vessel in sequence, and deionized water is added for preliminary mixing between each time;
and adding a magnetic composite material as a carrier into the mixture of the essential oil, and carrying out magnetic stirring and ultrasonic treatment by an ultrasonic oscillator to obtain a semi-emulsified compound essential oil system.
6. The preparation method of the acaricidal bacteriostatic agent according to claim 5, wherein the magnetic composite materials are insoluble in water and have different shapes, the particle diameter of each magnetic composite material is 0.05-0.6 mm, and a hole or a roar is arranged in each magnetic composite material.
7. Use of the acaricide bacteriostat according to any one of claims 1 to 6 in the preparation of daily necessities.
8. The application of the mite-killing bacteriostatic agent in preparing daily necessities according to claim 7, wherein the daily necessities comprise laundry soap, mite-killing laundry detergent, mite-killing bag, mite-killing spray, pet detergent, air-conditioning bactericide, washing machine detergent and environment detergent.
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CN112335691A (en) * 2020-10-23 2021-02-09 北京茂思商贸有限公司 Antibacterial and acarid-removing composite essential oil
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CN111567575A (en) * 2020-05-16 2020-08-25 无锡市伙伴日化科技有限公司 Environment-friendly sterilizing and mite-killing agent and preparation method thereof
CN112335691A (en) * 2020-10-23 2021-02-09 北京茂思商贸有限公司 Antibacterial and acarid-removing composite essential oil
CN114272296A (en) * 2021-11-23 2022-04-05 广州国色天香生物科技有限公司 Plant compound essential oil for removing mites and acnes and preparation method thereof

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