CN108935536A - A kind of natural drug and preparation method thereof inhibiting wawter bloom - Google Patents

A kind of natural drug and preparation method thereof inhibiting wawter bloom Download PDF

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CN108935536A
CN108935536A CN201810924901.8A CN201810924901A CN108935536A CN 108935536 A CN108935536 A CN 108935536A CN 201810924901 A CN201810924901 A CN 201810924901A CN 108935536 A CN108935536 A CN 108935536A
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water
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wawter bloom
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覃静欣
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash

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Abstract

The present invention provides a kind of natural drug and preparation method thereof for inhibiting wawter bloom, after being prepared into reagent by alginic acid, fruit of glossy privet extracting solution, orange peel extraction, Siberian cocklebur extracting solution, ferulic acid, D-alanine, D-Ser, water, neutralization bamboo vinegar, the hydrodictyon reticulatum measured with half times is mixed with.Hydrodictyon reticulatum is very strong to the inhibiting effect of Bloom-causing Algal, and removal efficiency is very high, inhibits the outburst of wawter bloom using the allelochemical collaboration in the fruit of glossy privet and orange peel extraction, inhibits the photosynthesis of Bloom-causing Algal；The substance mutually inhibited is Secondary Metabolite Production in Plants, there is the suitable pathways into environment, will not influence animal and people in plant interior accumulation.Inhibition wawter bloom natural drug prepared by the present invention is environmentally protective, while having broad-spectrum antibacterial effect, can effectively inhibit wawter bloom, and purifying water body has good economic benefit.

Description

A kind of natural drug and preparation method thereof inhibiting wawter bloom
Technical field
The invention belongs to environmental protection technical field, in particular to a kind of natural drug for inhibiting wawter bloom and its preparation Method.
Background technique
The most common result of water eutrophication is exactly the wawter bloom phenomenon for causing to be formed due to algal bloom.Algae Most of class of class has to form the type of harmful water China, including green alga, dinoflagellate, hidden algae, the chrysophyceae etc. for belonging to Eukaryotic Algae, And belong to the cyanobacteria of prokaryotes.Some generation smell substances of these algae, some generation toxin, but the hair of cyanobacterial bloom Life is widest in area, and harm is maximum, also the most serious to the harm of human health.
Patent of invention CN201710812645.9, it is a kind of to can inhibit and the natural Chinese medicinal herb and its preparation of cyanobacterial bloom of tagging Method, the ingredient including following weight percents: the coptis 10 ~ 30%, radix bupleuri 10 ~ 25%, rheum officinale 5 ~ 20%, Schisandra chinensis 5 ~ 15%, kaladana 5 ~ 15%, Chinese Ixeris 5 ~ 15%, spina gleditsiae 5 ~ 10%, Herba Euphorbiae Humifusae 3 ~ 8%, ginkgo leaf 4 ~ 6%, lotus leaf 2 ~ 5%, Herba seu Fructus Physalis Minimae 2 ~ 5%；Score ratio is weighed, dries, is shredded and mixes by weight after above-mentioned Chinese medicine material is cleaned, is cleaned, is sterilized It is even；Soak in water, be heated to reflux after take filtrate；It adds water, be heated to reflux rear waste and take filtrate, it will be during filtrate be mixed twice Herbal medicine；There are significant Water extract and action of contace poison principle to algae using Plant Secondary Metabolites (allelochemical), in a variety of Herbal medicine presses Chinese pharmacology combination principle, reaches allelochemical low concentration collaboration inhibition and algae effect of tagging, fast with algal control speed, The advantages that safe and non-toxic.
Patent of invention CN201410366191.3, a method of inhibiting Cyanophyta microcystis kutz wawter bloom, including following step Rapid: (1) the nitrogen concentration of Cyanophyta microcystis kutz wawter bloom water body occurs for monitoring；(2) water body that cyanobacterial bloom occurs is continued Aeration, to maintain water body oxyty；(3) while aeration, carbon containing carbohydrate is added, addition concentration is according to water body total nitrogen concentration Depending on；(4) silicon compound is added while adding carbohydrate in water body；(5) after 2-3 days, and cyanobacterial bloom particle is big in water body Small to become smaller, water transparency improves；(6) at the 7-10 days, carbohydrate is added again, and after addition, water bloom of water body particle can become more Small, quantity can be less.After the inventive method, cyanobacterial bloom is effectively suppressed in water body, and water transparency improves, for Environment and ecological protection are all of great significance.
Allelopathy is that the new subdiscipline ecology of phytoecology biochemical (Ecological Biochemistry) is ground Study carefully a part of content.The definition that Allelopathic Effect in Plants most starts refer to plant by the specific secondary substance of Environment release from And on neighbouring other plants (containing microorganism and its own) the beneficial and harmful influence that generates of growth and development.Now, vegetalization Sense Effect study in fact expanded between all organisms and environment centered on plant by chemical substance be medium Chemical interaction.
Condition when algal bloom is mostly hot weather, using inhibit wawter bloom drug action time is short at high temperature, very It is easy to lose drug effect, simultaneously because freshwater microalgae self-protection is strong under high temperature action, so that drug can not hair more efficiently Wave inhibitory effect.
Summary of the invention
For drawbacks described above existing for existing inhibition wawter bloom drug, the present invention provide it is a kind of inhibit wawter bloom natural drug and Preparation method can effectively inhibit wawter bloom at high temperature.
The present invention is achieved by the following technical solutions:
A kind of natural drug inhibiting wawter bloom, the natural drug is prepared by following weight fraction ratio substance: brown alga Sour 5 ~ 10 parts, 3 ~ 5 parts of fruit of glossy privet extracting solution, 2 ~ 6 parts of orange peel extraction, 3 ~ 8 parts of Siberian cocklebur extracting solution, 5 ~ 10 parts of ferulic acid, D- third 3 ~ 8 parts of propylhomoserin, 10 ~ 15 parts of water, neutralizes 8 ~ 19 parts of bamboo vinegar, 16 ~ 54 parts of hydrodictyon reticulatum at 3 ~ 8 parts of D-Ser.
Injury of the present invention using the allelopathy of plant to cell membrane, by the target site on cell membrane, by allelopathic object The signal of matter stress is transmitted into the cell, so that hormone, Ions Absorption etc. are had an impact, and hormone, Ions Absorption and water The variation of point situation etc. necessarily causes the variation of plant cell division, photosynthesis etc., causes to generate inhibiting effect to plant；This Invention utilizes Secondary Metabolite Production in Plants: the n-butanol phase and water phase of the fruit of glossy privet, Siberian cocklebur Aqueous extracts and orange peel extraction inhibit Fresh water bloom micro algae growth resists high temperature capabilities by alignic promotion blade face stomata absorption, citrus peel extract, so that Natural drug of the invention is also able to maintain inhibitory activity under hot weather, persistently and is rapidly achieved the effect for inhibiting algal bloom Fruit；
Hydrodictyon reticulatum algal control is that certain allelopathic anti-algal substance is discharged by hydrodictyon reticulatum to realize, the release of this allelochemical and water Amount of algae is related in body, and in non-outbreak period frustule negligible amounts, the allelochemical of hydrodictyon reticulatum secretion is also relatively fewer, when Alga cells number starts to increase, and when having the tendency that outburst, Bloom-causing Algal will induce hydrodictyon reticulatum to release a large amount of allelochemical, from And contain this growth, prevent blue algae bloom.Hydrodictyon reticulatum is very strong to the inhibiting effect of Bloom-causing Algal, and removal efficiency is very Height, inhibiting rate are up to 98%；In addition to Bloom-causing Algal growth can be effectively suppressed, there are also stronger nitrogen phosphorus ligands abilities for hydrodictyon reticulatum, thus It can inherently solve the problems, such as water eutrophication, play the role for the treatment of both manifestation and root cause of disease to eutrophication water；
Because the Subjective and Objective of allelopathy is Secondary Metabolite Production in Plants, animal and people are not influenced, while being had into environment Suitable pathways, will not be safe and efficient in plant interior accumulation, so that natural drug of the invention is green and healthy；Bamboo vinegar, glossy privet Sub- extracting solution, orange peel extraction and the interaction of Siberian cocklebur extracting solution, can not only enhance allelopathy, while having broad-spectrum antibacterial With the effect for walking quickly and keeping away pest, the multifunctionality of tip killing agent of the present invention is increased.
The present invention also provides the preparation methods of the natural drug of the inhibition wawter bloom, are prepared by following steps :
(1) take 5 ~ 10 parts of alginic acid, 3 ~ 5 parts of fruit of glossy privet extracting solution, 3 ~ 8 parts of Siberian cocklebur extracting solution be uniformly mixed obtain composition 1；
(2) 5 ~ 10 parts of ferulic acid, 3 ~ 8 parts of D-alanine, 3 ~ 8 parts of D-Ser, 2 ~ 6 parts of orange peel extraction, 10 ~ 15 parts of water are mixed It closes uniformly, is added after neutralizing 8 ~ 19 parts of bamboo vinegar, heating water bath adds composition 1 and be uniformly mixed to 30 ~ 40 DEG C, cooling To room temperature, composition 2 is obtained；
(3) composition 2 is mixed with 16 ~ 54 parts of hydrodictyon reticulatum, the natural drug for inhibiting wawter bloom can be obtained.
As a further improvement of the present invention, the hydrodictyon reticulatum additional amount is 0.4 ~ 0.6 times of 2 mass of composition.
As a further improvement of the present invention, the neutralization bamboo vinegar additional amount is fruit of glossy privet extracting solution, tangerine peel extraction The quality summation of liquid and Siberian cocklebur extracting solution.
As a further improvement of the present invention, the fruit of glossy privet extract concentration is 0.4 ~ 0.6g/mL, by following steps It is prepared:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 3 ~ 5 times of mass concentrations are 60% is added, room temperature leaching is sealed in wide-mouth bottle Bubble carries out 1 hour of ultrasound assisted extraction daily, carries out filtering removing raw material powder for soak after impregnating for 2 week, obtain Fruit of glossy privet leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extraction for being prepared into that concentration is 0.4 ~ 0.6g/mL is added in deionized water Liquid.
Effective allelochemical is PEARLITOL 25C in fruit of glossy privet extracting solution, has Thief zone dehydration property, can destroy cell knot Structure reduces cellular esterases vigor, to inhibit the synthesis of photosynthetic pigments；The principle active component oleanolic acid of the fruit of glossy privet is listed in Broad-spectrum antibiotic, to the skilful bacterium of large intestine, typhoid bacillus, hemolytic streptococcus, staphylococcus aureus, not ingeniously silly Shigella has significantly Effect is resisted, chloramphenicol especially is even stronger than to the inhibiting effect of golden yellow Xun grape ball mattress, typhoid bacillus.
As a further improvement of the present invention, the orange peel extraction concentration for 6 ~ 10g/L is prepared by the following steps and :
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition It, filtered filtrate is concentrated into the orange peel extraction that concentration is 6 ~ 10g/L.
Bioactive substance such as flavonoids, terpene, ferulic acid rich in etc. in orange peel extraction, these substances have The characteristics such as antibacterial, antiviral, while these substances have Water extract effect to the growth of microorganism and plant；Research finds tangerine There is the substance inhibited to plant chlorophyll a content in skin can be passed through brown by spraying citrus peel extract to wawter bloom Alginic acid and fruit of glossy privet extracting solution destroy eucaryotic cell structure effect, so that citrus peel extract is inhibited the synthesis of cyanobacteria chlorophyll, to reach Inhibit blue algae growth effect；Citrus peel extract can reduce influence of the high temperature to allelopathic Ingredients Active, so that day prepared by the present invention Right drug can also have good drug effect at high temperature.
As a further improvement of the present invention, the Siberian cocklebur extract concentration is 0.1 ~ 0.3g/mL, by following steps system It is standby and obtain:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 5 ~ 10 times of water enchroachment (invasion)s are added in gained powder Steep and vibrate, filtered after ultrasonic wave extraction 20min after 72h, Siberian cocklebur extracting solution can be obtained, be diluted with water to concentration be 0.1 ~ 0.3g/ml.
The allelochemical of Siberian cocklebur extract mainly inhibits the photosynthesis of tender tip, also has certain suppression to non-photosynthetic effect Production is used, and makes natural drug of the invention that can play a role on daytime and night.
As a further improvement of the present invention, the D-alanine and D-Ser concentration are 1 ~ 3mmol/L.
As a further improvement of the present invention, the neutralization bamboo vinegar is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca (OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
Bamboo vinegar is a kind of mixture being made of various ingredients such as organic acid, phenols, ketone, alcohols and cruel classes, dilution It can inhibit bioactivity at 100 times or less, while have the function of restraining and sterilizing bacteria, walking quickly and keeping away pest；Bamboo vinegar is to sterilization and allelopathic Inhibit that also there is synergistic effect, reduces the raw material usage amount of tip killing agent prepared by the present invention, and inhibitory effect is constant；After neutralization Bamboo vinegar eliminate the harmful components such as organic acid and tar, function and effect are better than raw bamboo vinegar liquid.
Beneficial effects of the present invention:
1, obtained by the present invention is mixed with using natural botanical extraction liquid, amino acid D type isomers and ferulic acid and alginic acid The natural drug for inhibiting wawter bloom, can quickly inhibit blue algae growth, citrus peel extract can make natural drug effective component of the invention It also can normally play a role at high temperature, lasting medicine；Bamboo vinegar can enhance the effect of allelopathic ingredient, use a small amount of component Identical effect can be reached, do not generate medicament residue, it is harmless, it is safe and healthy.
2, the present invention inhibits the life of cyanobacteria using fruit of glossy privet extracting solution, Siberian cocklebur extracting solution to the Water extract effect of tender tip Long, the Subjective and Objective of interaction is plant, interaction be plant secondary metabolite, work is not generated to human body With, while having the suitable pathways into environment；Natural extracting solution described in another aspect has plant pathogen, pest certain Inhibition and avoidance effect can inhibit pathogenic bacteria to infect water body, substantially increase raw material availability.
3, the natural drug prepared by the present invention for inhibiting wawter bloom reduces the usage amount of hydrodictyon reticulatum, the tolerance of the drug of raising Property and snap action performance, can also rapidly enter in Cells of Blue-green Algae to play a role at high temperature and carry out Water extract, have fine Economic benefit.
Specific embodiment
Below with reference to embodiment, the present invention is further described.
Embodiment 1
(1) take 5 parts of alginic acid, 5 parts of fruit of glossy privet extracting solution, 3 parts of Siberian cocklebur extracting solution be uniformly mixed obtain composition 1；
(2) 5 parts of ferulic acid, 3 parts of D-alanine, 3 parts of D-Ser, 2 parts of orange peel extraction, 10 parts of water are uniformly mixed, are added After neutralizing 10 parts of bamboo vinegar, heating water bath adds composition 1 and is uniformly mixed, be cooled to room temperature, obtain composition to 30 DEG C 2；
(3) composition 2 is mixed with 19 parts of hydrodictyon reticulatum, the natural drug for inhibiting wawter bloom can be obtained.
The fruit of glossy privet extract concentration is 0.4g/mL, is prepared by the following steps and obtains:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 3 times of mass concentrations are 60% is added, soak at room temperature is sealed in wide-mouth bottle, 1 hour of ultrasound assisted extraction is carried out daily, is carried out filtering removing raw material powder for soak after impregnating for 2 week, is obtained female Loyal sub- leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extracting solution for being prepared into that concentration is 0.4g/mL is added in deionized water.
The orange peel extraction concentration is 6g/L is prepared by the following steps and obtains:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition It, filtered filtrate is concentrated into the orange peel extraction that concentration is 6g/L.
The Siberian cocklebur extract concentration is 0.1g/mL, is prepared by the following steps and obtains:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 5 times of water enchroachment (invasion) bubbles are added simultaneously in gained powder Oscillation filters after 72h after ultrasonic wave extraction 20min, Siberian cocklebur extracting solution can be obtained, and is diluted with water to concentration as 0.1g/ml i.e. It can.
The D-alanine and D-Ser concentration is 1mmol/L.
The neutralization bamboo vinegar is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca (OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
Embodiment 2
(1) take 10 parts of alginic acid, 3 parts of fruit of glossy privet extracting solution, 8 parts of Siberian cocklebur extracting solution be uniformly mixed obtain composition 1；
(2) 10 parts of ferulic acid, 3 parts of D-alanine, 8 parts of D-Ser, 6 parts of orange peel extraction, 15 parts of water are uniformly mixed, are added After neutralizing 17 parts of bamboo vinegar, heating water bath adds composition 1 and is uniformly mixed, be cooled to room temperature, obtain composition to 40 DEG C 2；
(3) composition 2 is mixed with 48 parts of hydrodictyon reticulatum, the natural drug for inhibiting wawter bloom can be obtained.
The fruit of glossy privet extract concentration is 0.6g/mL, is prepared by the following steps and obtains:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 5 times of mass concentrations are 60% is added, soak at room temperature is sealed in wide-mouth bottle, 1 hour of ultrasound assisted extraction is carried out daily, is carried out filtering removing raw material powder for soak after impregnating for 2 week, is obtained female Loyal sub- leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extracting solution for being prepared into that concentration is 0.6g/mL is added in deionized water.
The orange peel extraction concentration is 10g/L is prepared by the following steps and obtains:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition It, filtered filtrate is concentrated into the orange peel extraction that concentration is 10g/L.
The Siberian cocklebur extract concentration is 0.2g/mL, is prepared by the following steps and obtains:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 10 times of water enchroachment (invasion) bubbles are added in gained powder And vibrate, it is filtered after ultrasonic wave extraction 20min after 72h, Siberian cocklebur extracting solution can be obtained, being diluted with water to concentration is 0.3g/ml ?.
The D-alanine and D-Ser concentration is 3mmol/L.
The neutralization bamboo vinegar is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca (OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
Embodiment 3
(1) take 8 parts of alginic acid, 4 parts of fruit of glossy privet extracting solution, 6 parts of Siberian cocklebur extracting solution be uniformly mixed obtain composition 1；
(2) 8 parts of ferulic acid, 6 parts of D-alanine, 6 parts of D-Ser, 4 parts of orange peel extraction, 12 parts of water are uniformly mixed, are added After neutralizing 14 parts of bamboo vinegar, heating water bath adds composition 1 and is uniformly mixed, be cooled to room temperature, obtain group to 30 ~ 40 DEG C Close object 2；
(3) composition 2 is mixed with 34 parts of hydrodictyon reticulatum, the natural drug for inhibiting wawter bloom can be obtained.
The fruit of glossy privet extract concentration is 0.5g/mL, is prepared by the following steps and obtains:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 4 times of mass concentrations are 60% is added, soak at room temperature is sealed in wide-mouth bottle, 1 hour of ultrasound assisted extraction is carried out daily, is carried out filtering removing raw material powder for soak after impregnating for 2 week, is obtained female Loyal sub- leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extracting solution for being prepared into that concentration is 0.5g/mL is added in deionized water.
The orange peel extraction concentration is 8g/L is prepared by the following steps and obtains:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition It, filtered filtrate is concentrated into the orange peel extraction that concentration is 8g/L.
The Siberian cocklebur extract concentration is 0.2g/mL, is prepared by the following steps and obtains:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 8 times of water enchroachment (invasion) bubbles are added simultaneously in gained powder Oscillation filters after 72h after ultrasonic wave extraction 20min, Siberian cocklebur extracting solution can be obtained, and is diluted with water to concentration as 0.2g/mL i.e. It can.
The D-alanine and D-Ser concentration is 2mmol/L.
The neutralization bamboo vinegar is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca (OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
Test example 1
Water sample is tested when taking certain ditch cyanobacterial bloom, and frustule is in exponential phase of growth at this time, and cyanobacteria quantity is up to after measured 1.68×108Cells/L, water body is dark green, natural drug prepared by above-described embodiment 3 is sprayed onto water body, cyanobacteria Abatement amount changes over time as shown in table 1.
Table 1
As shown in Table 1, cut rate is up to 51.7% after adding medicament 12h, and visually observing water body is in green, for 24 hours rear cyanobacteria cut rate Just reach 75.6%, visually observe water sample in light green color, 48h completes 98% removal, visually observes water sample in white green, cyanobacteria Substantially dead, 72h removal rate 99.9%, water body is completely removed in shallow white, cyanobacteria.Description of test, inhibition prepared by the present invention The natural drug of wawter bloom can inhibit from source and cyanobacterial bloom of tagging, and removal speed is fast, and effect is obvious, low in cost, nothing Poison is harmless, without secondary pollution, the drawbacks of can be used for administering large area breakout of cyanobacteria blooms, make up the prior art.
Above embodiments are only exemplary embodiment of the present invention, are not used in the limitation present invention, protection scope of the present invention It is defined by the claims.Those skilled in the art can make various repair to the present invention within the spirit and scope of the present invention Change or equivalent replacement, this modification or equivalent replacement also should be regarded as being within the scope of the present invention.

Claims (9)

1. a kind of natural drug for inhibiting wawter bloom, which is characterized in that be prepared by following weight fraction ratio substance: alginic acid 5 ~ 10 parts, 3 ~ 5 parts of fruit of glossy privet extracting solution, 2 ~ 6 parts of orange peel extraction, 3 ~ 8 parts of Siberian cocklebur extracting solution, 5 ~ 10 parts of ferulic acid, D-alanine 3 ~ 8 parts, 3 ~ 8 parts of D-Ser, 10 ~ 15 parts of water, neutralization 8 ~ 19 parts of bamboo vinegar, 16 ~ 54 parts of hydrodictyon reticulatum.
2. inhibiting the preparation method of the natural drug of wawter bloom as described in claim 1, which is characterized in that by following steps system It is standby and obtain:
(1) take 5 ~ 10 parts of alginic acid, 3 ~ 5 parts of fruit of glossy privet extracting solution, 3 ~ 8 parts of Siberian cocklebur extracting solution be uniformly mixed obtain composition 1；
(2) 5 ~ 10 parts of ferulic acid, 3 ~ 8 parts of D-alanine, 3 ~ 8 parts of D-Ser, 2 ~ 6 parts of orange peel extraction, 10 ~ 15 parts of water are mixed It closes uniformly, is added after neutralizing 8 ~ 19 parts of bamboo vinegar, heating water bath adds composition 1 and be uniformly mixed to 30 ~ 40 DEG C, cooling To room temperature, composition 2 is obtained；
(3) composition 2 is mixed with 16 ~ 54 parts of hydrodictyon reticulatum, the natural drug for inhibiting wawter bloom can be obtained.
3. the preparation method of the natural drug according to claim 2 for inhibiting wawter bloom, which is characterized in that the neutralization bamboo Vinegar liquid additional amount is the quality summation of fruit of glossy privet extracting solution, orange peel extraction and Siberian cocklebur extracting solution.
4. the preparation method of the natural drug according to claim 2 for inhibiting wawter bloom, which is characterized in that the hydrodictyon reticulatum Additional amount is 0.4 ~ 0.6 times of 2 mass of composition.
5. the preparation method of the natural drug according to claim 2 for inhibiting wawter bloom, which is characterized in that the fruit of glossy privet Extract concentration is 0.4 ~ 0.6g/mL, is prepared by the following steps and obtains:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 3 ~ 5 times of mass concentrations are 60% is added, room temperature leaching is sealed in wide-mouth bottle Bubble carries out 1 hour of ultrasound assisted extraction daily, carries out filtering removing raw material powder for soak after impregnating for 2 week, obtain Fruit of glossy privet leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extraction for being prepared into that concentration is 0.4 ~ 0.6g/mL is added in deionized water Liquid.
6. the preparation method of the natural drug according to claim 2 for inhibiting wawter bloom, which is characterized in that the tangerine peel mentions Take liquid concentration for 6 ~ 10g/L is prepared by the following steps and obtains:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition It, filtered filtrate is concentrated into the orange peel extraction that concentration is 6 ~ 10g/L.
7. the preparation method of the natural drug according to claim 2 for inhibiting wawter bloom, which is characterized in that the Siberian cocklebur mentions Taking liquid concentration is 0.1 ~ 0.3g/mL, is prepared by the following steps and obtains:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 5 ~ 10 times of water enchroachment (invasion)s are added in gained powder Steep and vibrate, filtered after ultrasonic wave extraction 20min after 72h, Siberian cocklebur extracting solution can be obtained, be diluted with water to concentration be 0.1 ~ 0.3g/ml.
8. the preparation method of the natural drug according to claim 2 for inhibiting wawter bloom, which is characterized in that third ammonia of D- Acid and D-Ser concentration are 1 ~ 3mmol/L.
9. the preparation method of the natural drug according to claim 2 for inhibiting wawter bloom, which is characterized in that the neutralization bamboo Vinegar liquid is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca (OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
CN201810924901.8A 2018-08-14 2018-08-14 A kind of natural drug and preparation method thereof inhibiting wawter bloom Withdrawn CN108935536A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109896601A (en) * 2019-03-06 2019-06-18 上海盛安消防工程有限公司 A kind of fire demand water inorganic agent and its preparation process
CN110558346A (en) * 2019-09-18 2019-12-13 南京泽生环保科技有限公司 algae inhibitor containing terrestrial plant capable of being hydroponically cultured

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109896601A (en) * 2019-03-06 2019-06-18 上海盛安消防工程有限公司 A kind of fire demand water inorganic agent and its preparation process
CN110558346A (en) * 2019-09-18 2019-12-13 南京泽生环保科技有限公司 algae inhibitor containing terrestrial plant capable of being hydroponically cultured

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Application publication date: 20181207