CN109090132A - A kind of natural weeding bacteriostatic agent and preparation method thereof - Google Patents
A kind of natural weeding bacteriostatic agent and preparation method thereof Download PDFInfo
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- CN109090132A CN109090132A CN201810923990.4A CN201810923990A CN109090132A CN 109090132 A CN109090132 A CN 109090132A CN 201810923990 A CN201810923990 A CN 201810923990A CN 109090132 A CN109090132 A CN 109090132A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
Abstract
The present invention provides a kind of natural weeding bacteriostatic agent and preparation method thereof, and the weeding bacteriostatic agent is prepared by following substance: glufosinate-ammonium, fruit of glossy privet extracting solution, orange peel extraction, Siberian cocklebur extracting solution, ferulic acid, D-alanine, D-Ser, water, neutralizes bamboo vinegar, organosilicon cream, ethylhydroxyethylcellulose at alginic acid.The photosynthesis of weeds and cell is inhibited to increase to inhibit its growth using the allelochemical in the fruit of glossy privet and orange peel extraction；The substance of weeding is Secondary Metabolite Production in Plants, there is the suitable pathways into environment, will not influence animal and the absorption of people in plant interior accumulation.It is environmentally protective that the present invention prepares herbicide agent, while having the function of broad-spectrum antibacterial and walking quickly and keeping away pest, has good economic benefit.
Description
Technical field
The invention belongs to herbicide technology field, in particular to a kind of natural weeding bacteriostatic agent and preparation method thereof.
Background technique
Weeds can win honour for crops, strive fertilizer, strive water, therefore farmland weed causes very big damage to production estimation every year
It loses.On the other hand to ensure that ornamental plant from insect pest, makes ornamental plant quickly grow up healthy and sound, mostly use the mode of spraying insecticide anti-
Control ornamental plant insect pest.But pesticide contains toxicity more, easily pollutes to surrounding enviroment such as air, and passes through prevention and treatment of spraying insecticide
Ornamental plant insect pest can not only cause insect pest dead, will also kill useful animals, be unfavorable for environmental protection and the ecological balance.In addition, pesticide
It is volatile after being sprayed on ornamental plant, so that pesticide only has drug effect over a period to come, it is unable to reach long-term, thorough prevention and treatment
The effect of insect pest.Chemical synthetic pesticide gains great popularity due to the features such as efficient, quick-acting, insecticidal spectrum is wide, this makes botanical pesticide
It studies and uses and once fall into low ebb.However, with the use of chemical pesticide, drawback is also gradually revealed, such as " 3R "
Problem (residual, drug resistance, the wildness again of pest).Botanical pesticide because of it efficiently, low toxicity, low-residual, selectivity is high, does harm to object not
The features such as being also easy to produce drug resistance and the concern for causing people again, become the hot spot of current pesticide research.
Patent of invention CN201810269538.0, a kind of antibacterial particle of the efficient herbicide pesticide of safety and environmental protection and its preparation side
Method, the raw material including following parts by weight: 10 ~ 14 parts of vegetalitas weeding additive, sustained release banket 40 ~ 50 parts, 5 ~ 7 parts of vegetable oil,
1 ~ 2 part of sodium humate, 7 ~ 11 parts of desinsection auxiliary agent, weeding increase property 0.1 ~ 0.4 part of agent, 1 ~ 3 part of white carbon black, 4 ~ 6 parts of modified bamboo powdered carbon,
3 ~ 5 parts of swelling binder.The present invention has antibacterial desinsection, weeding, adjustment soil multiple function, and effect is lasting, is effectively prevented and treated
Frequently seen plants disease, Environmental Safety are efficient, environmentally friendly and be not easy to produce resistance；Preparation method provided by the invention simultaneously,
Its lower production costs, technique are concise, practical value with higher and good application prospect.
Patent of invention CN201610864489.6, a kind of warehouse with can antibacterial herbicide and preparation method thereof, described removes
Careless agent includes each component of following mass fraction: 10 ~ 25 parts of glufosinate-ammonium, 10 ~ 30 parts of Semen Strychni extract, Fructus Kochiae extract 1 ~
10 parts, 0.8 ~ 3.5 part of surfactant, 0.4 ~ 3 part of thickener, 0.4 ~ 3 part of solvent, 1 ~ 2 part of defoaming agent, 0.8 ~ 4 part of bentonite,
1 ~ 5 part of copper sulfate monohydrate.The present invention also provides a kind of warehouse with can antibacterial herbicide preparation method, herbicidal effect of the present invention
Good, preparation method is simple, easy to spread.
In the prior art, herbicide use condition is mostly hot weather, and action time is short at high temperature for herbicide, it is easy to
Drug effect is lost, simultaneously because high temperature action blade face stomata is closed, herbicide can not performance herbicidal effect more efficiently.
Summary of the invention
For drawbacks described above existing for existing herbicide, the present invention provides a kind of natural bacteriostatic herbicide and its preparation side
Method.
The present invention is achieved by the following technical solutions:
A kind of natural weeding bacteriostatic agent, the weeding is antibacterial and is prepared by following weight fraction ratio substance: glufosinate-ammonium 5
~ 8 parts, 5 ~ 10 parts of alginic acid, 3 ~ 5 parts of fruit of glossy privet extracting solution, 2 ~ 6 parts of orange peel extraction, 3 ~ 8 parts of Siberian cocklebur extracting solution, ferulic acid 5 ~
10 parts, 3 ~ 8 parts of D-alanine, 3 ~ 8 parts of D-Ser, 10 ~ 15 parts of water, neutralization 13 ~ 27 parts of bamboo vinegar, 1 ~ 2 part of organosilicon cream, second
1 ~ 3 part of ethylhydroxyethylcellulose.
Injury of the present invention using the allelopathy of plant to cell membrane, by the target site on cell membrane, by allelopathic object
The signal of matter stress is transmitted into the cell, so that hormone, Ions Absorption etc. are had an impact, and hormone, Ions Absorption and water
The variation of point situation etc. necessarily causes the variation of plant cell division, photosynthesis etc., causes to generate inhibiting effect to plant；This
Invention utilizes Secondary Metabolite Production in Plants: the n-butanol phase and water phase of the fruit of glossy privet, Siberian cocklebur Aqueous extracts and orange peel extraction inhibit
Weed growth resists high temperature capabilities by alignic promotion blade face stomata absorption, citrus peel extract, so that of the invention
Tip killing agent is also able to maintain under hot weather kills tip activity, persistently and is rapidly achieved inhibition weed growth effect；Because allelopathic is made
Subjective and Objective is Secondary Metabolite Production in Plants, does not influence animal and people, while having the suitable pathways into environment, Bu Hui
Plant interior accumulation, so that tip killing agent of the invention is green and healthy, it is safe and efficient；Bamboo vinegar, fruit of glossy privet extracting solution, tangerine peel extract
Liquid and the interaction of Siberian cocklebur extracting solution, can not only enhance allelopathy, while having the function of broad-spectrum antibacterial and walking quickly and keeping away pest,
To Lan Shi achorion, Microsporon audouini, microsporum ferrugineum, ulotrichy microsporum, star nocar
The fungies such as bacterium have different degrees of inhibiting effect, increase the multifunctionality of tip killing agent of the present invention.
The present invention also provides the preparation methods of the natural weeding bacteriostatic agent, are prepared by following steps:
(1) by ratio of weight and the number of copies, take 5 ~ 8 parts of glufosinate-ammonium, 5 ~ 10 parts of alginic acid, 3 ~ 5 parts of fruit of glossy privet extracting solution, Siberian cocklebur extracting solution 3 ~
8 parts and 1 ~ 2 part of organosilicon cream are uniformly mixed and obtain herbicidal constituents；
(2) by ratio of weight and the number of copies, by 5 ~ 10 parts of ferulic acid, 3 ~ 8 parts of D-alanine, 3 ~ 8 parts of D-Ser, orange peel extraction 2 ~ 6
Part, 10 ~ 15 parts of water be uniformly mixed, be added after neutralizing 13 ~ 27 parts of bamboo vinegar and 1 ~ 3 part of ethylhydroxyethylcellulose, heating water bath
It to 30 ~ 40 DEG C, adds herbicidal constituents and is uniformly mixed, weeding bacteriostatic agent can be obtained after being cooled to room temperature.
As a further improvement of the present invention, the neutralization bamboo vinegar additional amount is fruit of glossy privet extracting solution, tangerine peel extraction
The quality summation of liquid and Siberian cocklebur extracting solution.
As a further improvement of the present invention, the fruit of glossy privet extract concentration is 0.4 ~ 0.6g/mL, by following steps
It is prepared:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 3 ~ 5 times of mass concentrations are 60% is added, room temperature leaching is sealed in wide-mouth bottle
Bubble carries out 1 hour of ultrasound assisted extraction daily, carries out filtering removing raw material powder for soak after impregnating for 2 week, obtain
Fruit of glossy privet leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often
Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens
It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extraction for being prepared into that concentration is 0.4 ~ 0.6g/mL is added in deionized water
Liquid.
Effective allelochemical is PEARLITOL 25C in fruit of glossy privet extracting solution, has Thief zone dehydration property, can destroy cell knot
Structure reduces cellular esterases vigor, to inhibit the synthesis of photosynthetic pigments；The principle active component oleanolic acid of the fruit of glossy privet is listed in
Broad-spectrum antibiotic, to the skilful bacterium of large intestine, typhoid bacillus, hemolytic streptococcus, staphylococcus aureus, not ingeniously silly Shigella has significantly
Effect is resisted, chloramphenicol especially is even stronger than to the inhibiting effect of golden yellow Xun grape ball mattress, typhoid bacillus.
As a further improvement of the present invention, the orange peel extraction concentration for 6 ~ 10g/L is prepared by the following steps and
:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition
It, filtered filtrate is concentrated into the orange peel extraction that concentration is 6 ~ 10g/L.
Bioactive substance such as flavonoids, terpene, ferulic acid rich in etc. in orange peel extraction, these substances have
The characteristics such as antibacterial, antiviral, while these substances have Water extract effect to the growth of microorganism and plant；Research finds tangerine
There is the substance inhibited to plant chlorophyll a content in skin can be passed through brown by spraying citrus peel extract to tender tip
Alginic acid and fruit of glossy privet extracting solution destroy eucaryotic cell structure effect, so that citrus peel extract is inhibited the synthesis of weeds chlorophyll, to reach
Kill tip effect；Citrus peel extract can reduce influence of the high temperature to allelopathic Ingredients Active, so that herbicide prepared by the present invention is in height
Also there can be good drug effect under temperature.
As a further improvement of the present invention, the Siberian cocklebur extract concentration is 0.1 ~ 0.3g/mL, by following steps system
It is standby and obtain:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 5 ~ 10 times of water enchroachment (invasion)s are added in gained powder
Steep and vibrate, filtered after ultrasonic wave extraction 20min after 72h, Siberian cocklebur extracting solution can be obtained, be diluted with water to concentration be 0.1 ~
0.3g/ml.
The allelochemical of Siberian cocklebur extract mainly inhibits the photosynthesis of tender tip, also has certain suppression to non-photosynthetic effect
Production is used, and makes tip killing agent of the invention that can play a role on daytime and night.
As a further improvement of the present invention, the D-alanine and D-Ser concentration are 1 ~ 3mmol/L.
As a further improvement of the present invention, the neutralization bamboo vinegar is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to 2 powder of a small amount of Ca (OH), is filtered after standing 1h, adds a small amount of Ca
(OH) 2 powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
Bamboo vinegar is a kind of mixture being made of various ingredients such as organic acid, phenols, ketone, alcohols and cruel classes, dilution
It can inhibit bioactivity at 100 times or less, while have the function of restraining and sterilizing bacteria, walking quickly and keeping away pest；Bamboo vinegar is to desinsection and allelopathic
Inhibit that also there is synergistic effect, reduces the raw material usage amount of herbicide prepared by the present invention, and herbicidal effect is constant；After neutralization
Bamboo vinegar eliminate the harmful components such as organic acid and tar, function and effect are better than raw bamboo vinegar liquid.
Beneficial effects of the present invention:
1, obtained by the present invention is mixed with using natural botanical extraction liquid, amino acid D type isomers and ferulic acid and alginic acid
Herbicide, can quickly inhibit weed growth, and citrus peel extract can make herbicide effective component of the invention at high temperature also can be just
Often play a role, lasting medicine；Bamboo vinegar can enhance the effect of herbicidal constituents, can also reach identical effect using a small amount of component
Fruit does not generate medicament residue, harmless, safe and healthy.
2, the present invention inhibits the life of weeds using fruit of glossy privet extracting solution, Siberian cocklebur extracting solution to the Water extract effect of tender tip
Long, the Subjective and Objective of interaction is plant, interaction be plant secondary metabolite, work is not generated to human body
With, while having the suitable pathways into environment；Natural extracting solution described in another aspect has plant pathogen, pest certain
Inhibition and avoidance effect can inhibit pathogenic bacteria to infect fruit tree and vegetable, reduce pest, substantially increase raw material availability.
Specific embodiment
Below with reference to embodiment, the present invention is further described.
Embodiment 1
(1) by ratio of weight and the number of copies, 8 parts of glufosinate-ammonium, 5 parts of alginic acid, 3 parts of fruit of glossy privet extracting solution, 3 parts of Siberian cocklebur extracting solution and organic are taken
1 part of silicon cream, which is uniformly mixed, obtains herbicidal constituents；
(2) by ratio of weight and the number of copies, by 5 parts of ferulic acid, 3 parts of D-alanine, 3 parts of D-Ser, 2 parts of orange peel extraction, 10 parts of water
It is uniformly mixed, is added after neutralizing 16 parts and 1 part of ethylhydroxyethylcellulose of bamboo vinegar, heating water bath adds weeding to 30 DEG C
Ingredient is simultaneously uniformly mixed, and weeding bacteriostatic agent can be obtained after being cooled to room temperature.
The fruit of glossy privet extract concentration is 0.4g/mL, is prepared by the following steps and obtains:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 3 times of mass concentrations are 60% is added, soak at room temperature is sealed in wide-mouth bottle,
1 hour of ultrasound assisted extraction is carried out daily, is carried out filtering removing raw material powder for soak after impregnating for 2 week, is obtained female
Loyal sub- leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often
Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens
It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extracting solution for being prepared into that concentration is 0.4g/mL is added in deionized water.
The orange peel extraction concentration is 6g/L is prepared by the following steps and obtains:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition
It, filtered filtrate is concentrated into the orange peel extraction that concentration is 6g/L.
The Siberian cocklebur extract concentration is 0.1g/mL, is prepared by the following steps and obtains:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 5 times of water enchroachment (invasion) bubbles are added simultaneously in gained powder
Oscillation filters after 72h after ultrasonic wave extraction 20min, Siberian cocklebur extracting solution can be obtained, and is diluted with water to concentration as 0.1g/ml i.e.
It can.
The D-alanine and D-Ser concentration is 1mmol/L.
The neutralization bamboo vinegar is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca
(OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
Embodiment 2
(1) by ratio of weight and the number of copies, 5 parts of glufosinate-ammonium, 10 parts of alginic acid, 5 parts of fruit of glossy privet extracting solution, 8 parts of Siberian cocklebur extracting solution are taken and is had
2 parts of machine silicon cream, which is uniformly mixed, obtains herbicidal constituents；
(2) by ratio of weight and the number of copies, by 10 parts of ferulic acid, 8 parts of D-alanine, 8 parts of D-Ser, 6 parts of orange peel extraction, 15 parts of water
It is uniformly mixed, is added after neutralizing 24 parts and 3 parts of ethylhydroxyethylcellulose of bamboo vinegar, heating water bath adds weeding to 40 DEG C
Ingredient is simultaneously uniformly mixed, and weeding bacteriostatic agent can be obtained after being cooled to room temperature.
The fruit of glossy privet extract concentration is 0.6g/mL, is prepared by the following steps and obtains:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 5 times of mass concentrations are 60% is added, soak at room temperature is sealed in wide-mouth bottle,
1 hour of ultrasound assisted extraction is carried out daily, is carried out filtering removing raw material powder for soak after impregnating for 2 week, is obtained female
Loyal sub- leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often
Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens
It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extracting solution for being prepared into that concentration is 0.6g/mL is added in deionized water.
The orange peel extraction concentration is 10g/L is prepared by the following steps and obtains:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition
It, filtered filtrate is concentrated into the orange peel extraction that concentration is 10g/L.
The Siberian cocklebur extract concentration is 0.2g/mL, is prepared by the following steps and obtains:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 10 times of water enchroachment (invasion) bubbles are added in gained powder
And vibrate, it is filtered after ultrasonic wave extraction 20min after 72h, Siberian cocklebur extracting solution can be obtained, being diluted with water to concentration is 0.3g/ml
?.
The D-alanine and D-Ser concentration is 3mmol/L.
The neutralization bamboo vinegar is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca
(OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
Embodiment 3
(1) by ratio of weight and the number of copies, 7 parts of glufosinate-ammonium, 6 parts of alginic acid, 4 parts of fruit of glossy privet extracting solution, 5 parts of Siberian cocklebur extracting solution and organic are taken
2 parts of silicon cream, which is uniformly mixed, obtains herbicidal constituents；
(2) by ratio of weight and the number of copies, by 8 parts of ferulic acid, 5 parts of D-alanine, 4 parts of D-Ser, 4 parts of orange peel extraction, 11 parts of water
It is uniformly mixed, is added after neutralizing 20 parts and 2 parts of ethylhydroxyethylcellulose of bamboo vinegar, heating water bath adds weeding to 38 DEG C
Ingredient is simultaneously uniformly mixed, and weeding bacteriostatic agent can be obtained after being cooled to room temperature.
The fruit of glossy privet extract concentration is 0.5g/mL, is prepared by the following steps and obtains:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 4 times of mass concentrations are 60% is added, soak at room temperature is sealed in wide-mouth bottle,
1 hour of ultrasound assisted extraction is carried out daily, is carried out filtering removing raw material powder for soak after impregnating for 2 week, is obtained female
Loyal sub- leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often
Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens
It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extracting solution for being prepared into that concentration is 0.5g/mL is added in deionized water.
The orange peel extraction concentration is 8g/L is prepared by the following steps and obtains:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition
It, filtered filtrate is concentrated into the orange peel extraction that concentration is 8g/L.
The Siberian cocklebur extract concentration is 0.2g/mL, is prepared by the following steps and obtains:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 8 times of water enchroachment (invasion) bubbles are added simultaneously in gained powder
Oscillation filters after 72h after ultrasonic wave extraction 20min, Siberian cocklebur extracting solution can be obtained, and is diluted with water to concentration as 0.2g/mL i.e.
It can.
The D-alanine and D-Ser concentration is 2mmol/L.
The neutralization bamboo vinegar is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca
(OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
Bacteriostatic test
It is compareed using common glufosinate-ammonium herbicide, 3 gained herbicide of embodiment is that experimental group carries out antibacterial inspection.
The preparation of culture medium: weighing beef extract 5g and be dissolved in water 800mL, and peptone 10g dissolution is added, casein is added
34g dissolution adds NaCl 5g dissolution, is adjusted to pH7.6 ~ 7.8, is diluted to 1000mL.Agar powder 18g, high pressure sterilization is added
15min, when being cooled to 40 DEG C ~ 50 DEG C, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices solidifies, and 4 DEG C of refrigerators for placing no bacteria pollution are spare.
Strain: bacillus anthracis.
Control group antibacterial liquid is prepared: 1 part of common glufosinate-ammonium herbicide is dissolved in respectively in 10ml aseptic deionized water, it will
Liquor strength is diluted respectively, is 1:2,1:4,1:8,1:16,1:32 with stoste ratio.
Experimental group antibacterial liquid is prepared: a herbicide of the present invention is dissolved in respectively in 10ml aseptic deionized water, by medicine
Liquid concentration is diluted respectively, is 1:2,1:4,1:8,1:16,1:32 with stoste ratio.Clump count is shown in 1 after culture.
Table 1
As shown in Table 1, there is good fungistatic effect using the obtained product of the present invention.
Field trial
Reagent agent: 3 kinds of herbicides in embodiment 1 ~ 3.
Comparison medicament: 25% flazasulfuron water dispersible granules (commercially available), 48% trifluralin ec (commercially available), 30% diformazan penta
Happy spirit suspending agent (commercially available).
For trying lawn: Bermuda grass.Bermuda grass was 3~5 leaf phases, was grown fine, robust plant, without other pest and disease damages.
Control object: rhizoma alismatis, nutgrass flatsedge, apple, bog pondweed, arrowhead, difformed galingale herb, herba stellariae mediae, herba digitariae, herba setariae viridis, barnyard grass, Cowhells
The lawns such as grass gesture weeds.
Test method: according to the area of experimental plot, good various medicaments of precise, and after being watered dilution, using bearing
Formula sprayer carries out even spraying, the dedicated Fan spray head of sprinkler selection herbicide.When spraying, it should be noted that uniformly spray medical fluid
The phenomenon that being applied in experimental plot, accomplishing no drain spray, more sprays.
20d, 40d observe weeds death condition, and the activity of weeding of more various medicaments after medicine respectively after test.This
Outside, whether the growing state that also observe lawn in 1 ~ 15d after medicine, have phytotoxicity to investigate medicament to lawn.
The embodiment of the present invention preparation Herbicidal Testing Trials effect see the table below 2 (20d after medicine) and table 3 (40d after medicine).
2 field control effectiveness test result of table (20d after medicine)
3 field control effectiveness test result of table (40d after medicine)
As can be seen that the present invention has significantly gramineae weed, sedge weed and broadleaf weeds from table 2 and table 3
Preventive effect, it is building performance significant, and also the lasting period is long.
Above embodiments are only exemplary embodiment of the present invention, are not used in the limitation present invention, protection scope of the present invention
It is defined by the claims.Those skilled in the art can make various repair to the present invention within the spirit and scope of the present invention
Change or equivalent replacement, this modification or equivalent replacement also should be regarded as being within the scope of the present invention.
Claims (8)
1. a kind of natural weeding bacteriostatic agent, which is characterized in that the natural weeding bacteriostatic agent is by following weight fraction ratio object
Matter is prepared: 5 ~ 8 parts of glufosinate-ammonium, 5 ~ 10 parts of alginic acid, 3 ~ 5 parts of fruit of glossy privet extracting solution, 2 ~ 6 parts of orange peel extraction, Siberian cocklebur mention
It takes 3 ~ 8 parts of liquid, 5 ~ 10 parts of ferulic acid, 3 ~ 8 parts of D-alanine, 3 ~ 8 parts of D-Ser, 10 ~ 15 parts of water, neutralize bamboo vinegar 13 ~ 27
Part, 1 ~ 2 part of organosilicon cream, 1 ~ 3 part of ethylhydroxyethylcellulose.
2. the preparation method of natural weeding bacteriostatic agent as described in claim 1, which is characterized in that prepared by following steps
:
(1) by ratio of weight and the number of copies, take 5 ~ 8 parts of glufosinate-ammonium, 5 ~ 10 parts of alginic acid, 3 ~ 5 parts of fruit of glossy privet extracting solution, Siberian cocklebur extracting solution 3 ~
8 parts and 1 ~ 2 part of organosilicon cream are uniformly mixed and obtain herbicidal constituents；
(2) by ratio of weight and the number of copies, by 5 ~ 10 parts of ferulic acid, 3 ~ 8 parts of D-alanine, 3 ~ 8 parts of D-Ser, orange peel extraction 2 ~ 6
Part, 10 ~ 15 parts of water be uniformly mixed, be added after neutralizing 13 ~ 27 parts of bamboo vinegar and 1 ~ 3 part of ethylhydroxyethylcellulose, heating water bath
It to 30 ~ 40 DEG C, adds herbicidal constituents and is uniformly mixed, weeding bacteriostatic agent can be obtained after being cooled to room temperature.
3. the preparation method of natural weeding bacteriostatic agent according to claim 2, which is characterized in that the neutralization bamboo vinegar
Additional amount is glufosinate-ammonium, the quality summation of fruit of glossy privet extracting solution, orange peel extraction and Siberian cocklebur extracting solution.
4. the preparation method of natural weeding bacteriostatic agent according to claim 2, which is characterized in that the fruit of glossy privet extracts
Liquid concentration is 0.4 ~ 0.6g/mL, is prepared by the following steps and obtains:
(1) the smashed fruit of glossy privet is taken, the ethyl alcohol that its 3 ~ 5 times of mass concentrations are 60% is added, room temperature leaching is sealed in wide-mouth bottle
Bubble carries out 1 hour of ultrasound assisted extraction daily, carries out filtering removing raw material powder for soak after impregnating for 2 week, obtain
Fruit of glossy privet leaching liquor；
(2) by fruit of glossy privet leaching liquor rotary evaporation to no alcohol, medicinal extract is obtained, after water redissolves, carries out extraction 4 times with n-butanol, often
Secondary solvent volume is 1.5 times of water volume, obtains n-butanol phase, water phase；
(3) n-butanol phase and water phase are subjected to rotary evaporation, remove organic reagent, obtained medicinal extract and solution is in 60 DEG C of baking ovens
It dries to constant weight, the powder after drying is scraped, the fruit of glossy privet extraction for being prepared into that concentration is 0.4 ~ 0.6g/mL is added in deionized water
Liquid.
5. the preparation method of natural weeding bacteriostatic agent according to claim 2, which is characterized in that the orange peel extraction
Concentration is 6 ~ 10g/L is prepared by the following steps and obtains:
It is dried after tangerine peel is cut into small pieces, is mixed in the ratio that tangerine peel does 0.05kg/L deionized water, extract 4 under dark condition
It, filtered filtrate is concentrated into the orange peel extraction that concentration is 6 ~ 10g/L.
6. the preparation method of natural weeding bacteriostatic agent according to claim 2, which is characterized in that the Siberian cocklebur extracting solution
Concentration is 0.1 ~ 0.3g/mL, is prepared by the following steps and obtains:
It takes Siberian cocklebur complete stool to clean soil with clear water, crushed 40 meshes after natural drying, 5 ~ 10 times of water enchroachment (invasion)s are added in gained powder
Steep and vibrate, filtered after ultrasonic wave extraction 20min after 72h, Siberian cocklebur extracting solution can be obtained, be diluted with water to concentration be 0.1 ~
0.3g/ml.
7. the preparation method of natural weeding bacteriostatic agent according to claim 2, which is characterized in that the D-alanine and
D-Ser concentration is 1 ~ 3mmol/L.
8. the preparation method of natural weeding bacteriostatic agent according to claim 2, which is characterized in that the neutralization bamboo vinegar
It is prepared by the following steps and obtains:
Raw bamboo vinegar liquid is taken, is stirred continuously down and is slowly added to a small amount of Ca (OH)2Powder filters after standing 1h, adds a small amount of Ca
(OH)2Powder repeats above step until not having precipitating to generate, as neutralization bamboo vinegar.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113907069A (en) * | 2020-11-30 | 2022-01-11 | 南开沧州渤海新区绿色化工研究有限公司 | Glufosinate-ammonium moisturizing synergistic composition and preparation method thereof |
| CN116210721A (en) * | 2022-12-30 | 2023-06-06 | 盐城工学院 | Biomass charcoal seed coating agent and preparation method thereof |
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2018
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113907069A (en) * | 2020-11-30 | 2022-01-11 | 南开沧州渤海新区绿色化工研究有限公司 | Glufosinate-ammonium moisturizing synergistic composition and preparation method thereof |
| CN116210721A (en) * | 2022-12-30 | 2023-06-06 | 盐城工学院 | Biomass charcoal seed coating agent and preparation method thereof |
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Application publication date: 20181228 |