CN114424757B - Two-stage hatching method for improving hatching rate of Chinese softshell turtle eggs - Google Patents
Two-stage hatching method for improving hatching rate of Chinese softshell turtle eggs Download PDFInfo
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Abstract
The invention relates to a two-stage incubation method for improving the incubation rate of Chinese soft-shelled turtle eggs, which relates to the technical field of Chinese soft-shelled turtle breeding. When the soft-shelled turtle eggs are incubated for about 10 days in the first stage, a small amount of dry river sand is covered and the fresh-keeping film is used for keeping the water content of the soft-shelled turtle eggs, so that the water content requirement of the soft-shelled turtle eggs in the stage can be completely guaranteed, the soft-shelled turtle eggs are effectively prevented from directly entering the incubation room environment with the higher humidity in the second stage, and the early-stage embryo development stability is promoted. The selected hatching material is simple and easy to obtain, bad eggs are removed without a back-boiled medium in the hatching process, and labor and force are saved. The hatching area is clean, the operation is safe, and the environment is protected and the energy is saved.
Description
Technical Field
The invention relates to the technical field of Chinese softshell turtle breeding, in particular to a two-section hatching method for improving the hatching rate of Chinese softshell turtle eggs.
Background
The Chinese soft-shelled turtle belongs to the phylum of chordata, class of reptiles, order of Testudinate, family of Trionychidae, genus of Trionycis, species of Chinese soft-shelled turtle, commonly known as soft-shelled turtle and soft-shelled turtle, has delicate meat quality, delicious taste and unique medicinal and nutritional values. Turtle meat is rich in turtle shell polysaccharide, EPA + DHA, collagen and vitamin B17, has health care effects of resisting tumor, improving immunity, delaying aging and the like, is popular with consumers, is one of traditional aquatic products treasure in China, and currently, the breeding area in China reaches more than 170 mu, the total output in China reaches 32 million tons, and the total economic benefit reaches billions yuan. With the rapid development of the industry, the industrial scale is larger and larger, and the demand of the industry for high-quality seedlings is increasing day by day. At present, the Chinese soft-shelled turtle breeding method in China mainly comprises the steps of building parent soft-shelled turtle ponds, breeding 1-2 parent soft-shelled turtles per square meter, carrying out intensive culture, building spawning rooms on ridges of the parent soft-shelled turtles, paving 20cm of fine sand in the spawning rooms, and arranging slopes for the parent soft-shelled turtles to enter and lay eggs conveniently; by simulating natural incubation conditions, the eggs of the soft-shelled turtles are covered by media such as sand, vermiculite and the like, a certain humidity is maintained, and the young soft-shelled turtles are incubated.
However, with the expansion of hatching scale, the problems of the traditional hatching mode are continuously revealed, which is mainly shown in that (1) whether the newly produced turtle egg is fertilized or not and whether the fertilization is good or not are not easy to distinguish; (2) The fertilized egg embryo which is just produced is unstable, and the embryo is easily damaged by excessive manual operation at the stage, so that the embryo is damaged and damaged; (3) The newly collected turtle eggs have partial unfertilized eggs and eggs with poor fertilization, dead eggs, rotting and mildewing easily occur in the hatching process, and infected bacteria infected by other good fertilized eggs often cause a large amount of bad eggs; (4) The newly produced turtle eggs contain a large amount of water, the requirement on the water content of an incubation medium is not high, if the turtle eggs are directly placed in an incubation room with high humidity, the humidity of the incubation medium is easy to be overlarge in the initial incubation period, the air permeability of the medium is poor, water layers are easy to adhere to the surfaces of the eggs, and the turtle eggs are often choked to die, the incubation rate is low, and the like.
Based on the technical problems, the hatchability of the traditional Chinese soft-shelled turtle one-step hatching mode is only 70-85%, even lower. Therefore, innovative hatching methods are needed to increase hatchability and improve farmer production.
Disclosure of Invention
The invention aims to provide a two-stage hatching method for improving the hatching rate of Chinese soft-shelled turtles, which can obviously improve the hatching rate of the soft-shelled turtles and effectively improve the output value of farmers.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a two-stage hatching method for improving the hatching rate of Chinese softshell turtle eggs comprises the following steps:
In the parent turtle egg laying season, in the morning, turtle eggs are collected in an egg laying room in a sand scraping mode and transported to an incubation room;
step 2, hatching in the first stage
(1) First stage hatching house
Arranging a simple hatching room, and regulating the temperature of the hatching room at the first stage to be 28-33 ℃;
(2) Turtle egg hatching package
Firstly, laying a preservative film at the bottom of an incubation frame, and laying a layer of dry river sand on the preservative film; laying turtle eggs on the dried river sand in the hatching frame layer by layer until the turtle eggs are full; secondly, paving a layer of dry river sand on the turtle eggs in the hatching frame, covering the turtle eggs and the dry river sand by turning the preservative film, and compacting the preservative film by using a small amount of dry river sand; finally, labeling the hatching frame, and indicating the source, the acquisition time, the time of putting the eggs into a hatching room and other information of the soft-shelled turtle;
(3) Hatching
Stacking the packaged hatching frames layer by layer, placing the hatching frames in a first stage hatching room for hatching for 8-12 days, checking the color of embryo in soft-shelled turtle eggs, screening soft-shelled turtle eggs with normal embryo development when the embryo in the soft-shelled turtle eggs is full of blood filaments and the eggs are red, and entering a second stage hatching room for hatching;
(1) Second stage hatching room
Platform steps which are arranged in the second stage hatching room and are higher than the ground by a certain distance are used for placing hatching frames, seedling collecting ditches are formed among the platform steps, and a proper amount of water level is stored in the seedling collecting ditches;
(2) Turtle egg hatching package
Firstly, selecting an incubation frame to load turtle eggs for second-stage incubation, laying 2 layers of sponge at the bottom of the incubation frame, wherein the upper layer of sponge is provided with turtle egg positioning holes for placing the turtle eggs; secondly, after the first stage incubation, selecting turtle eggs with blood streak filled embryos and red eggs, removing white and damaged turtle eggs, and arranging the turtle eggs in positioning holes of the sponge according to the animal polar upward; vermiculite is selected as a hatching medium, and laid on the discharged turtle eggs; finally, stacking the hatching frames in a pile layer by layer, and placing the stacked hatching frames on the platform step for 10-13 layers of each pile;
(3) Hatching
In the second stage, the hatching temperature of the hatching room is controlled to be 28-33 ℃, the relative humidity of air in the hatching room is controlled to be 70-85% by utilizing a sprayer, and the humidity of a hatching medium vermiculite is controlled to be 8-12%; after incubation for 33-36 days, the young soft-shelled turtles are incubated and taken out of shells, enter a seedling collecting ditch, are fished out every day in batches, and are placed in a greenhouse for large-size young soft-shelled turtle cultivation, so that the incubation of the eggs of the Chinese soft-shelled turtles is completed.
As the preferred technical scheme of the invention, in the two-stage incubation method of the Chinese soft-shelled turtle eggs:
the size of the hatching frame is 60cm by 40cm by 10cm by length by width by height.
And 2, paving dry river sand with the thickness of 1-2cm on the fresh-keeping film, and paving dry river sand with the thickness of 1-2cm after paving turtle eggs layer by layer.
In the step 3, the flat steps for placing the hatching frames are 15-20cm higher than the ground and 1-1.5m wide, seedling collecting ditches with the depth of 15-20cm and the width of 20-50cm are formed between the flat steps, and the water level in the seedling collecting ditches is controlled to be 3-8cm. The thickness of the two layers of sponge is 0.8-1cm, and turtle egg positioning holes with 12-18mm diameter are arranged in the upper layer of sponge for placing turtle eggs. Vermiculite with the particle size of 2-4mm is selected as a hatching medium, and vermiculite with the thickness of 1-2cm is laid on the discharged turtle eggs.
According to the embryo development habit of the soft-shelled turtle eggs, the two-stage incubation method for the Chinese soft-shelled turtle eggs is adopted, so that early development damage of the embryos is reduced, unfertilized eggs and eggs with poor fertilization are effectively removed, the incubation humidity control of the soft-shelled turtle eggs at different periods is effectively controlled, the broken eggs and dead eggs in the incubation process are reduced, and the incubation rate is improved. Compared with the prior art, the invention has the beneficial effects that:
1. after the turtle eggs are hatched for about 10 days in the first stage, the turtle egg embryos are full of blood filaments, the eggs are red, the development of the embryos is easy to judge, normal-development fertilized eggs can be effectively and preferably selected, unfertilized eggs and fertilized eggs with poor embryo development are removed, the phenomenon that the unfertilized eggs and fertilized eggs with poor embryo development are necrotic and rotten in the hatching process is avoided to a great extent, the nearby normally-hatched fertilized eggs are infected with bacteria, a large number of dead eggs and bad eggs are caused, the hatching rate is greatly improved, the operation link of checking the bad eggs in the hatching process is omitted, and the labor intensity is reduced.
2. According to the invention, the eggs which are just collected are quickly framed and enter the first stage for incubation, so that the unstable fertilized egg embryo at the early development stage is stabilized, excessive manual operation at the stage is effectively avoided, the embryo damage at the early development stage is reduced, and the normal development of the fertilized egg embryo is promoted.
3. The method adopts the first stage of incubation for about 10 days, uses a small amount of dry river sand to cover and the preservative film to keep the water content of the turtle eggs, can completely ensure the water content requirement of the turtle egg incubation at the stage, effectively avoids directly entering the incubation room environment with higher humidity at the second stage, and promotes the stable development of early embryos.
4. The hatching materials selected by the invention mainly comprise a hatching room, an air conditioner, a heater, a hatching frame, sponge, dry river sand, vermiculite and the like, and are simple and easy to obtain; bad eggs are removed without a pouring medium in the hatching process, so that labor and force are saved. The hatching area is clean, the operation is safe, and the environment is protected and the energy is saved.
Drawings
FIG. 1 is a schematic structural diagram of a hatching frame used in the first and second stages of hatching.
Fig. 2 is a plan layout view of the hatching chamber in the second stage.
Fig. 3 isbase:Sub>A view frombase:Sub>A-base:Sub>A in fig. 2.
Fig. 4 is a top view of a sponge-loaded hatching frame.
Detailed Description
Referring to fig. 1-4, the present invention provides a two-stage hatching method for increasing the hatching rate of the eggs of trionyx sinensis, comprising the following steps:
In 5-9 months every year, the temperature reaches above 25 ℃, which is the spawning season of parent turtles, in the spawning room every morning, turtle eggs are collected in a sand-raking mode and are transported to an incubation room.
Step 2, hatching in the first stage
The embryo of the newly-produced turtle egg is unstable, the jolt, vibration and excessive manual operation of the turtle egg are required to be reduced as much as possible, the embryo is prevented from being damaged, and the newly-produced turtle egg has low requirement on the hatching humidity and cannot directly enter the hatching environment with overlarge humidity.
(1) First stage hatching room
The setting area is 15-20m 2 The simple hatching room is internally provided with 1 heater with the power of 2kw and 1 air conditioner with the power of 1.5, and the temperature of the hatching room in the first stage is regulated and controlled to be about 31 ℃.
(2) Turtle egg hatching package
The first stage incubation was performed by loading eggs in an incubation frame 1 (shown in fig. 1) having a length, width, and height of 60cm, 40cm, and 10cm. Firstly, a piece of preservative film with the length and the width of 1.5m and 1m is paved at the bottom of an incubation frame, and dry river sand with the thickness of about 1cm is paved on the preservative film. Laying turtle eggs layer by layer on dry river sand in an incubation frame until the incubation frame is full, wherein 600-700 turtle eggs can be contained in each frame. Secondly, paving dry river sand with the thickness of 1-2cm on the turtle eggs in the hatching frame, turning over the preservative film to cover the turtle eggs and the dry river sand, and compacting the preservative film by using a small amount of the dry river sand. Finally, labeling on the hatching frame, and noting the source, the collecting time, the time of placing the turtle eggs in a hatching room and other information of the turtle eggs.
(3) Hatching
Stacking the packaged incubation frames layer by layer, placing the incubation frames in a first stage incubation room, combining a heater and an air conditioner, controlling the temperature in the first stage incubation room to be about 31 ℃, incubating for about 10 days, checking the color of the embryo in the soft-shelled turtle egg, screening the soft-shelled turtle egg with the embryo developing normally when the embryo in the egg is found to be full of blood filaments and the egg is red, and entering a second stage incubation room for incubation.
(1) Second stage hatching room
As shown in FIGS. 2 and 3, the second stage hatching chamber 6 has an area of 40-50m 2 Installing 1 heater 9 with the power of 2kw, 2 air conditioners 10 with the power of 1.5 and 1 fan, and controlling the temperature of the hatching room to be about 31 ℃; platform steps 7 which are 15-20cm higher than the ground and 1-1.5m wide are arranged in the hatching room and used for placing hatching frames, seedling collecting ditches 8 which are 15-20cm deep and 20-50cm wide are formed between the platform steps, and the water level in the seedling collecting ditches is controlled to be about 5 cm.
(2) Turtle egg hatching package
First, selecting an incubation frame with the length, width, height of 60cm, 40cm and 10cm to load turtle eggs, carrying out second-stage incubation on the turtle eggs, laying 2 layers of sponges with the thickness of 0.8-1cm (as shown in figure 4) at the bottom of the incubation frame, wherein the area of the upper layer of sponges 4 is properly smaller than that of the lower layer of sponges 3, and 300 turtle egg positioning holes 5 with the diameter of about 15mm are formed in the upper layer of sponges 4 and are used for placing the turtle eggs. Secondly, after the first stage of incubation, turtle eggs with blood streak filled embryos and red eggs are screened out, the white and damaged turtle eggs are removed, and the turtle eggs are arranged in positioning holes of the sponge according to the upward direction of the animal. Vermiculite with the particle size of 2-4mm is selected as a hatching medium, and vermiculite with the thickness of 1-2cm is laid on the discharged turtle eggs; spraying vermiculite by a sprayer to ensure that the humidity of the incubated vermiculite is 8-12%. Finally, the hatching frames are stacked one on top of the other, 10-13 layers per stack (as shown in fig. 3), and the stacked hatching frames are placed on the platform step.
(3) Hatching
A temperature and humidity meter is arranged in the second stage hatching room, the temperature of the hatching room is controlled to be about 31 ℃ by utilizing a heater, an air conditioner and a fan, the relative humidity of air in the hatching room is controlled to be 70-85% by utilizing a sprayer, and the humidity of a hatching medium vermiculite is controlled to be 8-12%. After incubation for 33-36 days, the young soft-shelled turtles are incubated and taken out of shells, enter a seedling collecting ditch, are fished out every day in batches, and are placed in a greenhouse for large-size young soft-shelled turtle cultivation, so that the incubation of the eggs of the Chinese soft-shelled turtles is completed.
The two-stage hatching method of the Chinese soft shell turtle egg of the present invention is further described in detail with reference to the following examples.
Example 1:
in 2020, 13 parent turtle ponds with the area of 17.5 mu are arranged in a culture base of Xijia agricultural development Limited company in Anhui province, and a first-stage hatching room with the area of 20m is arranged 2 Second stage hatching room 50m 2 。
1. Parent turtle pool
Each parent turtle pool is 60m long, 15m wide and 900m in single mouth area 2 1350 parent turtles of 5 years old are bred in each pond, wherein 1200 female turtles and 150 male turtles are bred, and the ratio of male to female is 8: 1.
2. Spawning room
A spawning room is built between the two parent turtle ponds, the width of the spawning room is 2m, the length of the spawning room is 30m, and the total length is 60m 2 Each parent turtle pond spawning room is 30m 2 2 parent turtle entrances and exits of 1.5m x 0.2m are arranged at the bottom of each spawning room, and 1 slope of which the entrance and the exit of each spawning room are provided with 2m x 1.5m and the mesh size is 3cm so that the parent turtles can conveniently enter and exit the spawning rooms.
3. First stage incubation
The first stage hatching room is a brick wall tile roof with an area of 20m 2 1 heater with the power of 2kw and 1 air conditioner with the power of 1.5 are installed, and the indoor temperature of the hatching room is regulated and controlled to be about 31 ℃. Collecting ovum Trionycis from spawning room in the year 2020, 5/15/monthPackaging preservative film, dry river sand and the like, loading 600-700 turtle eggs in each hatching frame, labeling, noting information such as source, acquisition time, time of placing in a hatching room and the like, stacking 10 frames in each stack, allowing the turtle eggs to enter a first stage hatching room for 10 days, checking hatching conditions of the eggs, and allowing the turtle eggs to start to enter a second stage hatching after embryo in the eggs are full of red blood filaments.
4. Second stage incubation
The second stage hatching room is a brick wall tile roof with an area of 50m 2 And 1 heater with the power of 2kw, 2 air conditioners with the power of 1.5 and 1 fan are installed. The flat step can contain 1170 hatching frames at one time, 300 fertilized eggs with normal embryo development are arranged in each hatching frame, and 35.1 million fertilized eggs can be contained in total. Selecting normal-developing fertilized eggs from the first-stage hatching room in 25 days, 5 months and 2020, arranging the fertilized eggs on upper sponge positioning holes in a hatching frame according to the condition that the animal is arranged upwards, covering the fertilized eggs with vermiculite, stacking the fertilized eggs in the hatching room for hatching, regulating and controlling the indoor temperature of the hatching room to be about 31 ℃ through a heater and an air conditioner, spraying the vermiculite by using a sprayer to ensure that the humidity of the vermiculite is 8-12%, and controlling the relative air humidity of the hatching room to be 70-85% by using the sprayer; and (5) in 27 days 6 and 6 in 2020, the hatched young turtles enter seedling collecting grooves, and the hatched young turtles are collected every day, put in a greenhouse for cultivation and counted.
5. Hatchability calculation
Through statistics, 70 ten thousand fertilized eggs which enter the second stage of incubation in 2020 are accumulated, 68 ten thousand fertilized eggs are accumulated, and the incubation rate reaches 97.1%.
Comparative example
(1) Design of experiments
In 2019, in a breeding base of Xijia agricultural development limited company in Anhui province, 2 stacks of test hatching frames are arranged, and each stack is 10 frames. Wherein 10 hatching frames are selected, and the two-stage hatching method of the Chinese soft shell turtle eggs is adopted as a test group; the other 10 hatching frames are used as a control group by adopting a traditional Chinese soft-shelled turtle egg one-step hatching method.
7000 turtle eggs were collected from the same egg laying room in the morning of 6.2019 and 1.8.M. For the test group, collected turtle eggs are packed by 3100 eggs through an incubation frame, a preservative film and fine river sand, stacked in an incubation room at the first stage for incubation, and the temperature of the incubation room is controlled by a heater and an air conditioner to be about 31 ℃. After incubation for 10 days, the embryo of turtle egg is checked to be full of blood silk, the egg is red, 3000 fertilized eggs are screened out, the turtle egg is arranged on the positioning hole of sponge according to the animal's polar upward, and 2cm vermiculite is covered. The hatching frames are stacked in the second stage hatching room for 10 frames. The temperature of the hatching room is controlled to be about 31 ℃ by utilizing a heater, an air conditioner and a fan, vermiculite is sprayed by utilizing a sprayer every 10-15 days according to the humidity condition of the vermiculite, and the humidity is kept to be 8-12 percent.
And the control group adopts a traditional one-step hatching method, 3000 fertilized eggs are screened after the collected turtle eggs are placed for 1 day, the turtle eggs are directly arranged on positioning holes of sponge according to the animal polar direction, 2cm vermiculite is covered, the total frame is 10, and other hatching conditions (temperature and humidity of a hatching room and vermiculite humidity) and a hatching method are kept consistent with the second section hatching method of the test group.
(2) Results
After a hatching control test for 46 days, research results show that 2958 young turtles are hatched in the test group in total, the hatching rate is 98.6%, and the hatching time is 43-46 days; 2556 young turtles are hatched in the control group, the hatching rate is 85.2%, and the hatching time is 43-47 days.
By comparison it can be seen that: the hatching time difference is not obvious, but the hatching rate difference is larger, and the hatching rate of a test group is 13.4 percent higher than that of a control group, so that the two-stage hatching method for the Chinese soft-shelled turtle eggs is an innovative hatching method, the hatching rate can be obviously improved, and the output value of farmers is improved.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.
Claims (7)
1. A two-stage hatching method for improving the hatching rate of Chinese softshell turtle eggs is characterized by comprising the following steps:
step 1, collecting turtle eggs
In the parent turtle spawning season, turtle eggs are collected in a spawning room in a sand-raking mode every morning and are transported to an incubation room;
step 2, hatching in the first stage
(1) First stage hatching house
Arranging a simple hatching room, and regulating the temperature of the hatching room at the first stage to be 28-33 ℃;
(2) Turtle egg hatching package
Firstly, laying a preservative film at the bottom of an incubation frame, and laying a layer of dry river sand on the preservative film; laying turtle eggs on the dried river sand in the hatching frame layer by layer until the turtle eggs are full; secondly, laying a layer of dry river sand on the turtle eggs in the hatching frame, covering the turtle eggs and the dry river sand by using a preservative film in a turnover mode, and compacting the preservative film by using a small amount of dry river sand; finally, labeling the hatching frame, and noting three information of turtle egg source, turtle egg collection time and turtle egg placing time in a hatching room;
(3) Hatching
Stacking the packaged hatching frames layer by layer, placing in a first stage hatching room for hatching for 8-12 days, checking the color of embryo in turtle egg, when the embryo is full of blood silk and the egg is red, screening turtle egg with normal embryo development, and placing in a second stage hatching room for hatching;
step 3, second stage incubation
(1) Second stage hatching room
Platform steps which are arranged in the second stage hatching room and are higher than the ground by a certain distance are used for placing hatching frames, seedling collecting ditches are formed among the platform steps, and a proper amount of water level is stored in the seedling collecting ditches;
(2) Turtle egg hatching package
Firstly, selecting an incubation frame to load turtle eggs for second-stage incubation, laying 2 layers of sponge at the bottom of the incubation frame, wherein the upper layer of sponge is provided with turtle egg positioning holes for placing the turtle eggs; secondly, after the turtle eggs are hatched in the first stage, turtle eggs with blood strips and red eggs filled with embryos are screened out, the white and damaged turtle eggs are removed, and the turtle eggs are arranged in positioning holes of the sponge upwards according to the animal grade; vermiculite is selected as a hatching medium, and laid on the discharged turtle eggs; finally, stacking the hatching frames in a pile layer by layer, and placing the stacked hatching frames on the platform step for 10-13 layers of each pile;
(3) Hatching
In the second stage, the hatching temperature of the hatching room is controlled to be 28-33 ℃, the relative humidity of air in the hatching room is controlled to be 70-85% by utilizing a sprayer, and the humidity of a hatching medium vermiculite is controlled to be 8-12%; after incubation for 33-36 days, the young soft-shelled turtles are incubated out of shells in succession, enter a seedling collecting ditch, are fished out every day in batches, and are placed in a greenhouse for large-size young soft-shelled turtle cultivation, so that the incubation of the eggs of the Chinese soft-shelled turtles is completed.
2. The two-stage hatching method for increasing the hatching rate of eggs of trionyx sinensis according to claim 1, wherein the size of the hatching frame is 60cm by 40cm by 10cm by width by height.
3. The two-stage hatching method for improving the hatching rate of the eggs of Chinese soft-shelled turtles according to claim 1, wherein in the step 2, dry river sand with the thickness of 1-2cm is paved on the preservative film, and after the eggs of the soft-shelled turtles are paved layer by layer, dry river sand with the thickness of 1-2cm is paved on the eggs of the soft-shelled turtles.
4. The two-stage hatching method for improving the hatching rate of Chinese softshell turtle eggs according to claim 1, wherein the flat step for placing the hatching frame in the step 3 is 15-20cm higher than the ground and 1-1.5m wide.
5. The two-stage hatching method for improving the hatching rate of the eggs of Chinese soft-shelled turtles according to claim 1, wherein seedling collecting ditches with the depth of 15-20cm and the width of 20-50cm are formed between the platform steps in the step 3, and the water level in the seedling collecting ditches is controlled to be 3-8cm.
6. The two-stage hatching method for improving the hatching rate of Chinese softshell turtle eggs according to claim 1, wherein the thickness of the two layers of sponge laid in the step 3 is 0.8-1cm, and the upper layer of sponge is provided with turtle egg positioning holes with the aperture of 12-18mm for placing turtle eggs.
7. The two-stage hatching method for improving the hatching rate of Chinese softshell turtle eggs according to claim 1, wherein vermiculite with the grain diameter of 2-4mm is selected as a hatching medium in step 3, and the vermiculite with the thickness of 1-2cm is laid on the arranged turtle eggs.
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