CN114424757A - Two-stage hatching method for improving hatchability of Chinese softshell turtle eggs - Google Patents
Two-stage hatching method for improving hatchability of Chinese softshell turtle eggs Download PDFInfo
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Abstract
The invention relates to a two-stage incubation method for improving the incubation rate of Chinese soft-shelled turtle eggs, which relates to the technical field of Chinese soft-shelled turtle breeding. When the soft-shelled turtle eggs are incubated for about 10 days in the first stage, a small amount of dry river sand is covered and the fresh-keeping film is used for keeping the water content of the soft-shelled turtle eggs, so that the water content requirement of the soft-shelled turtle eggs in the stage can be completely guaranteed, the soft-shelled turtle eggs are effectively prevented from directly entering the incubation room environment with the higher humidity in the second stage, and the early-stage embryo development stability is promoted. The selected hatching material is simple and easy to obtain, bad eggs are removed without a rising medium in the hatching process, and labor and force are saved. The hatching area is clean, the operation is safe, and the environment is protected and the energy is saved.
Description
Technical Field
The invention relates to the technical field of Chinese softshell turtle breeding, in particular to a two-section hatching method for improving the hatching rate of Chinese softshell turtle eggs.
Background
The Chinese soft-shelled turtle belongs to the phylum chordata, class reptilia, order terrapin, family terrapin, genus trionyx, species of Chinese soft-shelled turtle, commonly known as soft-shelled turtle and soft-shelled turtle, has fine and smooth meat quality, delicious taste and unique medicinal and nutritional values. Turtle meat is rich in turtle shell polysaccharide, EPA + DHA, collagen and vitamin B17, has health care effects of resisting tumor, improving immunity, delaying aging and the like, is popular with consumers, is one of traditional aquatic products precious goods in China, has the national culture area of more than 170 mu and total national yield of 32 million tons at present, and has total economic benefit of billions of yuan. With the rapid development of the industry, the industrial scale is larger and larger, and the demand of the industry for high-quality seedlings is increasing day by day. At present, the Chinese soft-shelled turtle breeding method in China mainly comprises the steps of building parent soft-shelled turtle ponds, breeding 1-2 parent soft-shelled turtles per square meter, carrying out intensive culture, building spawning rooms on ridges of the parent soft-shelled turtles, paving 20cm of fine sand in the spawning rooms, and arranging slopes for the parent soft-shelled turtles to enter and lay eggs conveniently; by simulating natural incubation conditions, the eggs of the soft-shelled turtles are covered by media such as sand, vermiculite and the like, a certain humidity is maintained, and the young soft-shelled turtles are incubated.
However, with the expansion of hatching scale, the problems of the traditional hatching mode are continuously revealed, which is mainly shown in that (1) whether the newly produced turtle egg is fertilized or not and whether the fertilization is good or not are not easy to distinguish; (2) the embryo of the fertilized egg which is just produced is unstable, and the embryo is easily damaged by excessive manual operation at the stage, so that the embryo is damaged and damaged; (3) the newly collected turtle eggs have partial unfertilized eggs and eggs with poor fertilization, dead eggs, rotting and mildewing easily occur in the hatching process, and infected bacteria infected by other good fertilized eggs often cause a large amount of bad eggs; (4) the newly produced turtle eggs contain a large amount of water, the requirement on the water content of an incubation medium is not high, if the turtle eggs are directly placed in an incubation room with high humidity, the humidity of the incubation medium is easy to be overlarge in the initial incubation period, the air permeability of the medium is poor, water layers are easy to adhere to the surfaces of the eggs, and the turtle eggs are often suffocated to die, the incubation rate is low, and the like.
Based on the technical problems, the hatchability of the traditional Chinese soft-shelled turtle one-step hatching mode is only 70-85%, even lower. Therefore, innovative hatching methods are needed to increase hatchability and improve farmer productivity.
Disclosure of Invention
The invention aims to provide a two-stage hatching method for improving the hatching rate of Chinese soft-shelled turtles, which can obviously improve the hatching rate of the soft-shelled turtles and effectively improve the output value of farmers.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a two-stage hatching method for improving the hatching rate of Chinese softshell turtle eggs comprises the following steps:
In the spawning season of the young soft-shelled turtles, soft-shelled turtle eggs are collected in a spawning room in a sand-scraping mode every morning and transported to an incubation room;
step 2, hatching in the first stage
(1) First stage hatching house
Arranging a simple hatching room, and regulating the temperature of the hatching room at the first stage to be 28-33 ℃;
(2) turtle egg hatching package
Firstly, laying a preservative film at the bottom of an incubation frame, and laying a layer of dry river sand on the preservative film; laying turtle eggs on the dried river sand in the hatching frame layer by layer until the turtle eggs are full; secondly, laying a layer of dry river sand on the eggs of the soft-shelled turtles in the hatching frame, covering the soft-shelled turtle eggs and the dry river sand by using a preservative film in a turnover mode, and compacting the preservative film by using a small amount of dry river sand; finally, labeling the hatching frame, and noting the source, the acquisition time, the time of putting the turtle eggs into a hatching room and other information of the turtle eggs;
(3) hatching
Stacking the packaged hatching frames layer by layer, placing the hatching frames in a first stage hatching room for hatching for 8-12 days, checking the color of embryo in soft-shelled turtle eggs, screening soft-shelled turtle eggs with normal embryo development when the embryo in the soft-shelled turtle eggs is full of blood filaments and the eggs are red, and entering a second stage hatching room for hatching;
(1) Second stage hatching room
Platform steps which are arranged in the second stage hatching room and are higher than the ground by a certain distance are used for placing hatching frames, seedling collecting ditches are formed among the platform steps, and a proper amount of water level is stored in the seedling collecting ditches;
(2) turtle egg hatching package
Firstly, selecting an incubation frame to load turtle eggs for second-stage incubation, laying 2 layers of sponges at the bottom of the incubation frame, wherein turtle egg positioning holes are formed in the upper layer of sponges and used for placing the turtle eggs; secondly, after the turtle eggs are hatched in the first stage, turtle eggs with blood filaments and red eggs filled with embryos are screened out, white and damaged turtle eggs are removed, and the turtle eggs are arranged in positioning holes of sponges according to the animal polar upward direction; vermiculite is selected as a hatching medium, and laid on the well-arranged turtle eggs; finally, stacking the hatching frames in a pile layer by layer, and placing the stacked hatching frames on the platform step for 10-13 layers of each pile;
(3) hatching
Controlling the hatching temperature of the hatching room at 28-33 ℃ in the second stage, controlling the air relative humidity of the hatching room at 70-85% by using a sprayer, and controlling the vermiculite humidity of a hatching medium at 8-12%; after incubation for 33-36 days, the young soft-shelled turtles are incubated out of shells in succession, enter a seedling collecting ditch, are fished out every day in batches, and are placed in a greenhouse for large-size young soft-shelled turtle cultivation, so that the incubation of the eggs of the Chinese soft-shelled turtles is completed.
As the preferred technical scheme of the invention, in the two-stage incubation method of the Chinese soft-shelled turtle eggs:
the size of the hatching frame is 60cm by 40cm by 10 cm.
In the step 2, dry river sand with the thickness of 1-2cm is paved on the preservative film, and dry river sand with the thickness of 1-2cm is paved on turtle eggs layer by layer.
In the step 3, the flat steps for placing the hatching frames are 15-20cm higher than the ground and 1-1.5m wide, seedling collecting ditches with the depth of 15-20cm and the width of 20-50cm are formed between the flat steps, and the water level in the seedling collecting ditches is controlled to be 3-8 cm. The thickness of the two layers of sponge is 0.8-1cm, and turtle egg positioning holes with 12-18mm diameter are arranged in the upper layer of sponge for placing turtle eggs. Vermiculite with the particle size of 2-4mm is selected as a hatching medium, and vermiculite with the thickness of 1-2cm is laid on the discharged turtle eggs.
According to the embryo development habit of the soft-shelled turtle eggs, the two-stage incubation method for the Chinese soft-shelled turtle eggs is adopted, so that early development damage of the embryos is reduced, unfertilized eggs and eggs with poor fertilization are effectively removed, the incubation humidity control of the soft-shelled turtle eggs at different periods is effectively controlled, the broken eggs and dead eggs in the incubation process are reduced, and the incubation rate is improved. Compared with the prior art, the invention has the beneficial effects that:
1. after the turtle eggs are hatched for about 10 days in the first stage, the turtle egg embryos are full of blood filaments, the eggs are red, the development of the embryos is easy to judge, normal-development fertilized eggs can be effectively and preferably selected, unfertilized eggs and fertilized eggs with poor embryo development are removed, the phenomenon that the unfertilized eggs and fertilized eggs with poor embryo development are necrotic and rotten in the hatching process is avoided to a great extent, the nearby normally-hatched fertilized eggs are infected with bacteria, a large number of dead eggs and bad eggs are caused, the hatching rate is greatly improved, the operation link of checking the bad eggs in the hatching process is omitted, and the labor intensity is reduced.
2. According to the invention, the eggs which are just collected are quickly framed and enter the first stage for incubation, so that the unstable fertilized egg embryo at the early development stage is stabilized, excessive manual operation at the stage is effectively avoided, the embryo damage at the early development stage is reduced, and the normal development of the fertilized egg embryo is promoted.
3. The method adopts the first stage of incubation for about 10 days, uses a small amount of dry river sand to cover and the preservative film to keep the water content of the turtle eggs, can completely ensure the water content requirement of the turtle egg incubation at the stage, effectively avoids directly entering the incubation room environment with higher humidity at the second stage, and promotes the stable development of early embryos.
4. The hatching materials selected by the invention mainly comprise a hatching room, an air conditioner, a heater, a hatching frame, sponge, dry river sand, vermiculite and the like, and are simple and easy to obtain; bad eggs are removed without a pouring medium in the hatching process, so that labor and force are saved. The hatching area is clean, the operation is safe, and the environment is protected and the energy is saved.
Drawings
FIG. 1 is a schematic structural diagram of a hatching frame used in the first and second stages of hatching.
Fig. 2 is a plan layout view of the hatching chamber in the second stage.
Fig. 3 is a view from a-a in fig. 2.
Fig. 4 is a top view of a sponge-loaded hatching frame.
Detailed Description
Referring to fig. 1-4, the present invention provides a two-stage hatching method for increasing the hatching rate of the eggs of trionyx sinensis, comprising the following steps:
Collecting turtle eggs in a sand-raking mode in a spawning room every morning in 5-9 months at the temperature of over 25 ℃ every year, wherein the spawning season of the young turtles is the spawning season of the young turtles, and transporting the eggs to an incubation room.
Step 2, hatching in the first stage
The embryo of the newly-produced turtle egg is unstable, the jolt, vibration and excessive manual operation of the turtle egg are required to be reduced as much as possible, the embryo is prevented from being damaged, and the newly-produced turtle egg has low requirement on the hatching humidity and cannot directly enter the hatching environment with overlarge humidity.
(1) First stage hatching house
The setting area is 15-20m2The simple hatching room is internally provided with 1 heater with the power of 2kw and 1 air conditioner with the power of 1.5 p, and the temperature of the hatching room in the first stage is regulated and controlled to be about 31 ℃.
(2) Turtle egg hatching package
Turtle eggs are loaded in an incubation frame 1 (shown in figure 1) with the length, width and height of 60cm, 40cm and 10cm, and the first stage of incubation is carried out. Firstly, a piece of preservative film with the length and width of 1.5m by 1m is paved at the bottom of an incubation frame, and dry river sand with the thickness of about 1cm is paved on the preservative film. Laying turtle eggs layer by layer on dry river sand in an incubation frame until the incubation frame is full, and 600 eggs and 700 eggs can be filled in each frame. Secondly, paving a layer of dry river sand with the thickness of 1-2cm on the turtle eggs in the hatching frame, covering the turtle eggs and the dry river sand by using a preservative film in a turnover mode, and compacting the preservative film by using a small amount of dry river sand. Finally, labeling on the hatching frame, and noting the source, the collecting time, the time of placing the turtle eggs in a hatching room and other information of the turtle eggs.
(3) Hatching
Stacking the packaged incubation frames layer by layer, placing the incubation frames in a first stage incubation room, combining a heater and an air conditioner, controlling the temperature in the first stage incubation room to be about 31 ℃, incubating for about 10 days, checking the color of the embryo in the soft-shelled turtle egg, screening the soft-shelled turtle egg with the embryo developing normally when the embryo in the egg is found to be full of blood filaments and the egg is red, and entering a second stage incubation room for incubation.
(1) Second stage hatching room
As shown in FIGS. 2 and 3, the second stage hatching chamber 6 has an area of 40-50m2Installing 1 heater 9 with the power of 2kw, 2 air conditioners 10 with the power of 1.5 and 1 fan, and controlling the temperature of the hatching room to be about 31 ℃; platform steps 7 with a height of 15-20cm and a width of 1-1.5m are arranged in the hatching room and used for placing hatching frames, seedling collecting ditches 8 with a depth of 15-20cm and a width of 20-50cm are formed between the platform steps, and the water level in the seedling collecting ditches is controlled to be about 5cm。
(2) Turtle egg hatching package
Firstly, selecting an incubation frame with length, width and height of 60cm, 40cm and 10cm to load turtle eggs, carrying out second-stage incubation on the turtle eggs, laying 2 layers of sponges with thickness of 0.8-1cm (shown in figure 4) at the bottom of the incubation frame, wherein the area of an upper layer of the sponges 4 is properly smaller than that of a lower layer of the sponges 3, and 300 turtle egg positioning holes 5 with the diameter of about 15mm are arranged in the upper layer of the sponges 4 and used for placing the turtle eggs. Secondly, after the turtle eggs are hatched in the first stage, turtle eggs with blood filaments and red embryos are screened out, white embryos and damaged turtle eggs are removed, and the turtle eggs are arranged in positioning holes of sponges according to the condition that the animals are in an upward direction. Vermiculite with the particle size of 2-4mm is selected as a hatching medium, and vermiculite with the thickness of 1-2cm is laid on the discharged turtle eggs; spraying vermiculite by a sprayer to ensure that the humidity of the incubated vermiculite is 8-12%. Finally, the hatching frames are stacked one on top of the other, 10-13 layers per stack (as shown in fig. 3), and the stacked hatching frames are placed on the platform step.
(3) Hatching
A temperature and hygrometer is arranged in the second stage hatching room, the temperature of the hatching room is controlled to be about 31 ℃ by a heater, an air conditioner and a fan, the relative humidity of air in the hatching room is controlled to be 70-85% by a sprayer, and the humidity of the hatching medium vermiculite is controlled to be 8-12%. After incubation for 33-36 days, the young soft-shelled turtles are incubated out of shells in succession, enter a seedling collecting ditch, are fished out every day in batches, and are placed in a greenhouse for large-size young soft-shelled turtle cultivation, so that the incubation of the eggs of the Chinese soft-shelled turtles is completed.
The two-stage incubation method of trionyx sinensis eggs according to the present invention will be described in detail with reference to the following examples.
Example 1:
in 2020, 13 parent turtle ponds with the area of 17.5 mu are arranged in a culture base of Xijia agricultural development Limited company in Anhui province, and a first-stage hatching room with the area of 20m is arranged2Second stage hatching room 50m2。
1. Parent turtle pool
Each parent turtle pool is 60m long, 15m wide and 900m in single mouth area21350 parent turtles of 5 years old are bred in each pond, wherein 1200 female turtles and 150 male turtles are bred, and the ratio of male to female is 8: 1.
2. Spawning room
A spawning room is built between the two parent turtle ponds, the width of the spawning room is 2m, the length of the spawning room is 30m, and the total length is 60m2Each parent turtle pond spawning room is 30m2The bottom of each spawning room is provided with 2 parent turtle entrances and exits of 1.5m x 0.2m, and each spawning room entrance and exit is provided with 1 slope of 2m x 1.5m and 3cm mesh size for facilitating the parent turtles to enter and exit the spawning rooms.
3. First stage incubation
The first stage hatching room is a brick wall tile roof with an area of 20m 21 heater with the power of 2kw and 1 air conditioner with the power of 1.5 are installed, and the indoor temperature of the hatching room is regulated and controlled to be about 31 ℃. And 5, 15 days in 2020, 5 months, turtle eggs are collected from an egg laying room successively, the eggs are packaged by using preservative films, dry river sand and the like, 600 plus 700 turtle eggs are loaded in each hatching frame, labels are pasted, the source, the collection time, the time of putting the eggs into the hatching room and other information are noted, each pile of eggs is stacked for 10 frames, the turtle eggs enter the hatching room of the first stage for 10 days, the hatching condition of the eggs is checked, and when embryo blood filaments in the eggs are full of red, the eggs begin to enter the second stage for hatching in frames.
4. Second stage incubation
The second stage hatching room is a brick wall tile roof with an area of 50m2And 1 heater with the power of 2kw, 2 air conditioners with the power of 1.5 and 1 fan are installed. The flat step can contain 1170 hatching frames at one time, 300 fertilized eggs with normal embryo development are arranged in each hatching frame, and 35.1 million fertilized eggs can be contained in total. Selecting normal-developing fertilized eggs from the first-stage hatching room in 25 days, 5 months and 2020, arranging the fertilized eggs on upper sponge positioning holes in a hatching frame according to the condition that the animal is arranged upwards, covering the fertilized eggs with vermiculite, stacking the fertilized eggs in the hatching room for hatching, regulating and controlling the indoor temperature of the hatching room to be about 31 ℃ through a heater and an air conditioner, spraying the vermiculite by using a sprayer to ensure that the humidity of the vermiculite is 8-12%, and controlling the relative air humidity of the hatching room to be 70-85% by using the sprayer; and 5, in 27 days 6 and 27 in 2020, the hatched young soft-shelled turtles enter seedling collecting grooves, and the hatched young soft-shelled turtles are collected every day, put into a greenhouse for cultivation and counted.
5. Hatchability calculation
Through statistics, 70 ten thousand fertilized eggs which are accumulated to enter the second stage of incubation in 2020 are counted, 68 ten thousand fertilized eggs are accumulated, and the incubation rate reaches 97.1%.
Comparative example
(1) Design of experiments
In 2019, in a breeding base of Xijia agricultural development limited company in Anhui province, 2 stacks of test hatching frames are arranged, and each stack is 10 frames. Wherein 10 hatching frames are selected, and the two-stage hatching method of the Chinese soft shell turtle eggs is adopted as a test group; the other 10 hatching frames are used as a control group by adopting a traditional Chinese soft-shelled turtle egg one-step hatching method.
7000 turtle eggs were collected from the same spawning room in the morning of 6.1.2019. For the experimental group, collected turtle eggs are packed with 3100 eggs through an incubation frame, a preservative film and fine river sand, stacked in an incubation room at the first stage for incubation, and the temperature of the incubation room is controlled by a heater and an air conditioner to be about 31 ℃. After incubation for 10 days, the embryo of turtle egg is checked to be full of blood silk, the egg is red, 3000 fertilized eggs are screened out, the turtle egg is arranged on the positioning hole of the sponge according to the animal's polar upward, and 2cm vermiculite is covered. The hatching frames are stacked in the second stage hatching room for 10 frames. The temperature of the hatching room is controlled to be about 31 ℃ by a heater, an air conditioner and a fan, vermiculite is sprayed by a sprayer every 10 to 15 days according to the humidity of the vermiculite, and the humidity is kept to be 8 to 12 percent.
And the contrast group adopts a traditional one-step hatching method, 3000 fertilized eggs are screened out after the collected turtle eggs are placed for 1 day, the turtle eggs are directly arranged on the positioning holes of the sponge according to the animal polar direction, 2cm of vermiculite is covered, 10 frames are totally formed, and other hatching conditions (temperature and humidity of a hatching room and vermiculite humidity) and a hatching method are consistent with the second section hatching method of the test group.
(2) Results
After a hatching control test for 46 days, research results show that 2958 young turtles are hatched in the test group in total, the hatching rate is 98.6%, and the hatching time is 43-46 days; 2556 young turtles are hatched in the control group, the hatching rate is 85.2%, and the hatching time is 43-47 days.
By comparison it can be seen that: the hatching time difference is not obvious, but the hatching rate difference is large, and the hatching rate of a test group is 13.4% higher than that of a control group, so that the two-stage hatching method for the eggs of the Chinese soft-shelled turtles is an innovative hatching method, the hatching rate can be obviously improved, and the output value of farmers is improved.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.
Claims (7)
1. A two-stage hatching method for improving the hatching rate of Chinese softshell turtle eggs is characterized by comprising the following steps:
step 1, collecting turtle eggs
In the spawning season of the young soft-shelled turtles, soft-shelled turtle eggs are collected in a spawning room in a sand-scraping mode every morning and transported to an incubation room;
step 2, hatching in the first stage
(1) First stage hatching house
Arranging a simple hatching room, and regulating the temperature of the hatching room at the first stage to be 28-33 ℃;
(2) turtle egg hatching package
Firstly, laying a preservative film at the bottom of an incubation frame, and laying a layer of dry river sand on the preservative film; laying a layer of turtle eggs on the dry river sand in the hatching frame until the turtle eggs are full; secondly, laying a layer of dry river sand on the eggs of the soft-shelled turtles in the hatching frame, covering the soft-shelled turtle eggs and the dry river sand by using a preservative film in a turnover mode, and compacting the preservative film by using a small amount of dry river sand; finally, labeling the hatching frame, and indicating the source, the acquisition time, the time of putting the eggs into a hatching room and other information of the soft-shelled turtle;
(3) hatching
Stacking the packaged hatching frames layer by layer, placing the hatching frames in a first stage hatching room for hatching for 8-12 days, checking the color of embryo in soft-shelled turtle eggs, screening soft-shelled turtle eggs with normal embryo development when the embryo in the soft-shelled turtle eggs is full of blood filaments and the eggs are red, and entering a second stage hatching room for hatching;
step 3, second stage incubation
(1) Second stage hatching room
Platform steps which are arranged in the second stage hatching room and are higher than the ground by a certain distance are used for placing hatching frames, seedling collecting ditches are formed among the platform steps, and a proper amount of water level is stored in the seedling collecting ditches;
(2) turtle egg hatching package
Firstly, selecting an incubation frame to load turtle eggs for second-stage incubation, laying 2 layers of sponges at the bottom of the incubation frame, wherein turtle egg positioning holes are formed in the upper layer of sponges and used for placing the turtle eggs; secondly, after the turtle eggs are hatched in the first stage, turtle eggs with blood strips and red eggs filled with embryos are screened out, the white and damaged turtle eggs are removed, and the turtle eggs are arranged in positioning holes of the sponge upwards according to the animal grade; vermiculite is selected as a hatching medium, and laid on the discharged turtle eggs; finally, the hatching frames are stacked one on another in a stacking manner, and the stacked hatching frames are placed on the platform step every 10-13 stacks;
(3) hatching
Controlling the hatching temperature of the hatching room at 28-33 ℃ in the second stage, controlling the air relative humidity of the hatching room at 70-85% by using a sprayer, and controlling the vermiculite humidity of a hatching medium at 8-12%; after incubation for 33-36 days, the young soft-shelled turtles are incubated out of shells in succession, enter a seedling collecting ditch, are fished out every day in batches, and are placed in a greenhouse for large-size young soft-shelled turtle cultivation, so that the incubation of the eggs of the Chinese soft-shelled turtles is completed.
2. The two-stage hatching method for increasing hatchability of trionyx sinensis eggs according to claim 1, wherein the size of the hatching frame is 60cm by 40cm by 10 cm.
3. The two-stage hatching method for improving the hatching rate of the eggs of Chinese soft-shelled turtles according to claim 1, wherein in the step 2, dry river sand with the thickness of 1-2cm is paved on the fresh-keeping film, and after the eggs of the soft-shelled turtles are paved layer by layer, dry river sand with the thickness of 1-2cm is paved on the fresh-keeping film.
4. The two-stage hatching method for improving the hatching rate of the eggs of Chinese soft-shelled turtles according to claim 1, wherein the flat step for placing the hatching frame in the step 3 is 15-20cm higher than the ground and 1-1.5m wide.
5. The two-stage hatching method for improving the hatching rate of the eggs of Chinese soft-shelled turtles according to claim 1, wherein seedling collecting ditches with the depth of 15-20cm and the width of 20-50cm are formed between the platform steps in the step 3, and the water level in the seedling collecting ditches is controlled to be 3-8 cm.
6. The two-stage hatching method for improving the hatching rate of Chinese softshell turtle eggs according to claim 1, wherein the thickness of the two layers of sponge laid in the step 3 is 0.8-1cm, and the upper layer of sponge is provided with turtle egg positioning holes with the aperture of 12-18mm for placing turtle eggs.
7. The two-stage hatching method for improving the hatching rate of the eggs of Chinese soft-shelled turtles according to claim 1, wherein vermiculite with the particle size of 2-4mm is selected as a hatching medium in step 3, and the vermiculite with the thickness of 1-2cm is laid on the arranged eggs of the soft-shelled turtles.
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