CN114395584B - 表达方法 - Google Patents
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- CN114395584B CN114395584B CN202111508425.XA CN202111508425A CN114395584B CN 114395584 B CN114395584 B CN 114395584B CN 202111508425 A CN202111508425 A CN 202111508425A CN 114395584 B CN114395584 B CN 114395584B
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/3076—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties
- C07K16/3092—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties against tumour-associated mucins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
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| JP7032438B2 (ja) | 2017-12-11 | 2022-03-08 | 富士フイルム株式会社 | 動物細胞、動物細胞の製造方法および目的タンパク質の製造方法 |
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| CN109837277A (zh) * | 2019-03-01 | 2019-06-04 | 深圳生生凡非基因技术有限公司 | 一种cpgk嵌合型启动子、腺相关病毒载体及其应用 |
| WO2021107123A1 (ja) | 2019-11-29 | 2021-06-03 | 富士フイルム株式会社 | 細胞培養方法、抗体製造方法、有機酸除去方法、及び、抗体 |
| EP4276167A1 (en) | 2021-01-07 | 2023-11-15 | FUJIFILM Corporation | Cell selection method, device and device system |
| WO2022196710A1 (ja) | 2021-03-17 | 2022-09-22 | 富士フイルム株式会社 | 細胞培養方法、及び有用物質の製造方法 |
| CN112980852B (zh) * | 2021-05-18 | 2021-08-13 | 北京华芢生物技术有限公司 | 新型冠状病毒b.1.351南非突变株rbd的基因及其应用 |
| JP2024521784A (ja) | 2021-05-27 | 2024-06-04 | ヤンセン バイオテツク，インコーポレーテツド | 前立腺がんの治療のための組成物及び方法 |
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| WO2003093295A2 (en) * | 2002-04-30 | 2003-11-13 | University Of North Carolina At Chapel Hill | Secretion signal vectors |
| WO2012173344A2 (ko) * | 2011-06-13 | 2012-12-20 | 주식회사종근당 | Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법 |
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| US8029783B2 (en) * | 2005-02-02 | 2011-10-04 | Genentech, Inc. | DR5 antibodies and articles of manufacture containing same |
| EP1739179A1 (en) * | 2005-06-30 | 2007-01-03 | Octapharma AG | Serum-free stable transfection and production of recombinant human proteins in human cell lines |
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| CN101646775B (zh) * | 2006-12-28 | 2016-08-24 | 森托科尔奥索生物科技公司 | 用于产生脱唾液酸化免疫球蛋白的方法和载体 |
| KR101618767B1 (ko) * | 2008-09-02 | 2016-05-09 | 한국생명공학연구원 | 외래단백질을 고효율로 생산하는 리더서열 |
| CN102272160A (zh) * | 2008-12-31 | 2011-12-07 | 印度血清及疫苗有限公司 | 抗rhd单克隆抗体 |
| PE20160653A1 (es) | 2009-03-05 | 2016-07-24 | Abbvie Inc | Proteinas de union a il-17 |
| GB0905331D0 (en) * | 2009-03-27 | 2009-05-13 | Avecia Biolog Ltd | Expression process |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2003093295A2 (en) * | 2002-04-30 | 2003-11-13 | University Of North Carolina At Chapel Hill | Secretion signal vectors |
| WO2012173344A2 (ko) * | 2011-06-13 | 2012-12-20 | 주식회사종근당 | Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법 |
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| US11306323B2 (en) | 2022-04-19 |
| SG11201508447TA (en) | 2015-11-27 |
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| WO2014177826A1 (en) | 2014-11-06 |
| KR20160003705A (ko) | 2016-01-11 |
| US20180127777A1 (en) | 2018-05-10 |
| BR112015027373A2 (pt) | 2017-08-29 |
| EP2992104B1 (en) | 2019-04-17 |
| RU2015151626A (ru) | 2017-06-08 |
| US9873891B2 (en) | 2018-01-23 |
| JP2016517691A (ja) | 2016-06-20 |
| DK2992104T3 (da) | 2019-07-15 |
| US20160076051A1 (en) | 2016-03-17 |
| CA2910877A1 (en) | 2014-11-06 |
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