CN114375642A - Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition - Google Patents
Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition Download PDFInfo
- Publication number
- CN114375642A CN114375642A CN202111440145.XA CN202111440145A CN114375642A CN 114375642 A CN114375642 A CN 114375642A CN 202111440145 A CN202111440145 A CN 202111440145A CN 114375642 A CN114375642 A CN 114375642A
- Authority
- CN
- China
- Prior art keywords
- rice seeds
- seeds
- agent
- rice
- coating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 122
- 235000009566 rice Nutrition 0.000 title claims abstract description 122
- 230000035784 germination Effects 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000001301 oxygen Substances 0.000 title claims abstract description 23
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 23
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 121
- 239000011248 coating agent Substances 0.000 claims abstract description 101
- 238000000576 coating method Methods 0.000 claims abstract description 48
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000002156 mixing Methods 0.000 claims abstract description 26
- 238000003756 stirring Methods 0.000 claims abstract description 23
- 238000011282 treatment Methods 0.000 claims abstract description 17
- 238000001035 drying Methods 0.000 claims abstract description 8
- 238000004806 packaging method and process Methods 0.000 claims abstract description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 36
- 239000000706 filtrate Substances 0.000 claims description 35
- 238000001914 filtration Methods 0.000 claims description 20
- 238000002791 soaking Methods 0.000 claims description 20
- 239000000047 product Substances 0.000 claims description 14
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical group OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 12
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 238000003892 spreading Methods 0.000 claims description 7
- 230000007480 spreading Effects 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 239000003623 enhancer Substances 0.000 claims description 6
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 4
- 239000004343 Calcium peroxide Substances 0.000 claims description 4
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical group NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 claims description 4
- 240000003183 Manihot esculenta Species 0.000 claims description 4
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 230000000844 anti-bacterial effect Effects 0.000 claims description 4
- 239000003899 bactericide agent Substances 0.000 claims description 4
- LHJQIRIGXXHNLA-UHFFFAOYSA-N calcium peroxide Chemical group [Ca+2].[O-][O-] LHJQIRIGXXHNLA-UHFFFAOYSA-N 0.000 claims description 4
- 235000019402 calcium peroxide Nutrition 0.000 claims description 4
- YKBZOVFACRVRJN-UHFFFAOYSA-N dinotefuran Chemical group [O-][N+](=O)\N=C(/NC)NCC1CCOC1 YKBZOVFACRVRJN-UHFFFAOYSA-N 0.000 claims description 4
- 239000007888 film coating Substances 0.000 claims description 4
- 238000009501 film coating Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 4
- 239000011347 resin Substances 0.000 claims description 4
- 229920005989 resin Polymers 0.000 claims description 4
- 238000001223 reverse osmosis Methods 0.000 claims description 4
- 239000002893 slag Substances 0.000 claims description 4
- 235000010413 sodium alginate Nutrition 0.000 claims description 4
- 229940005550 sodium alginate Drugs 0.000 claims description 4
- 239000000661 sodium alginate Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 239000010902 straw Substances 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 239000002917 insecticide Substances 0.000 claims description 2
- 239000000575 pesticide Substances 0.000 claims description 2
- 239000008399 tap water Substances 0.000 claims description 2
- 235000020679 tap water Nutrition 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 5
- 206010021143 Hypoxia Diseases 0.000 abstract description 4
- 239000002689 soil Substances 0.000 abstract description 3
- 238000009825 accumulation Methods 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 24
- 238000010899 nucleation Methods 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 238000004131 Bayer process Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
Abstract
The invention discloses a method for improving the germination rate of direct-seeded rice seeds under a low-oxygen condition, which comprises the steps of respectively adding water into an inner wrapping film and an outer wrapping film and uniformly stirring to obtain an inner wrapping film agent and an outer wrapping film agent for later use; mixing and coating the rice seeds subjected to special pretreatment and an inner coating agent, naturally placing for 2-3 hours, mixing and coating the rice seeds and an outer coating agent after finishing, and placing the rice seeds and the outer coating agent in a low-temperature dryer for drying treatment after finishing; and (4) determining the water content of the dried coated rice seeds, and performing waterproof packaging and storage after the water content is qualified. The special rice seed pretreatment process is adopted, the mass ratio of the coating agent to the rice seeds is strictly limited to 1:1 when the coating is carried out, and the specially prepared coating agent is combined with the special rice seed pretreatment process, so that the germination rate, the germination index and the vigor index of the rice seeds under the low-oxygen condition can be effectively improved, and the mildew rate of the rice seeds is reduced. Solves the problems of oxygen deficiency caused by overlarge rainfall, water accumulation in the pits on the field surface and overlarge covering soil, and provides reliable guarantee for increasing the income and increasing the yield of the rice.
Description
Technical Field
The invention belongs to the technical field of agriculture, and discloses a method for improving the germination rate of direct-seeded rice seeds under a low-oxygen condition.
Background
Direct seeding of rice is a simple cultivation technique which appears from the last 80 th century, and is a technique for directly seeding rice seeds in a field to cultivate rice by omitting the operations of seedling raising and transplanting in the rice cultivation process. Compared with transplanted rice, the direct seeding technology of rice is popular with farmers because of the advantages of reducing labor intensity and saving production cost. According to incomplete statistics, the area of direct-seeded rice in China currently exceeds 200 ten thousand mu. According to the rule that foreign rice cropping technology develops along with industrialization and marketization, along with the improvement of the rice production scale degree in China and the change of rural labor force structure, the direct seeding technology is one of the important directions of rice cultivation in China in future.
Although the direct seeding cultivation of rice has many advantages, it is increasingly paid attention. However, the direct seeding cultivation of rice has corresponding problems, wherein uneven emergence caused by low germination rate is one of the serious obstacles influencing the high and stable yield of the direct seeding rice. The direct seeding rice seed germination rate is caused by various reasons, and the most common reasons are that rainfall is too large, water is accumulated in a pit on the field surface, and the covering soil is too thick to cause oxygen deficiency. Therefore, the method has important significance for improving the direct-seeded rice yield and reducing the rice production cost by improving the germination and seedling rate of the rice seeds under the condition of hypoxia stress.
Based on the above, the application provides a simple, convenient and efficient coating agent formula for improving the germination rate of the direct-seeded rice seeds under the condition of low oxygen stress.
Disclosure of Invention
In order to overcome the defects of the prior art, the application aims at providing a coating agent for rice seeds firstly and then providing a method for improving the germination rate of the direct-seeded rice seeds under the low-oxygen condition by applying the coating agent. After the seed coating agent prepared by the invention is used for coating rice seeds, the rice seeds are gradually decomposed when meeting water and slowly release oxygen, so that the germination rate of direct seeding of the seeds under the stress of low oxygen can be effectively improved. The method is realized by the following means:
a rice seed coating agent comprising:
an inner wrapping film and an outer covering film;
wherein the inner wrapping film comprises: 2.3-3.8 parts of a nutrient enhancer; 0.2-0.5 parts of an oxygen increasing agent; and 0.35-0.80 parts of water-retaining agent;
the outer coating film includes: 0.5-1.3 parts of an insecticide; 2-7 parts of a bactericide; 2-6 parts of an antifreezing agent; and 1-6 parts of a film forming agent.
Further, the nutrient enhancer is prepared by mixing straw powder and enzyme bacteria (Tamarie, manufacturers are Tamarie microbiological research institute) in a mass ratio of (85-95) to (5-15);
the oxygen increasing agent is calcium peroxide, and the water-retaining agent is cassava starch resin.
Further, the pesticide is dinotefuran, the bactericide is carbamate, and the antifreezing agent is ethylene glycol;
the film forming agent is prepared by mixing polyvinyl alcohol, sodium alginate and styrene according to the mass ratio of (2-5) to (1-2) to (0.1-0.5).
The invention also discloses an application of any one of the direct-seeded rice seed coating agents in improving the germination rate of direct-seeded rice seeds under a low-oxygen condition, which comprises the following steps:
(1) respectively adding water into the inner coating film and the outer coating film and uniformly stirring to obtain an inner coating agent and an outer coating film agent for later use;
(2) mixing and coating the pretreated rice seeds and the inner coating agent, naturally placing for 2-3 hours, mixing and coating the rice seeds and the outer coating agent after finishing, and placing the rice seeds and the outer coating agent in a low-temperature dryer for drying treatment after finishing;
(3) measuring the water content of the dried coated rice seeds;
(4) and (5) waterproof packaging and storing the qualified seeds.
Further, the mass ratio of the inner wrapping film to water in the step (1) is 1:1.5, and the mass ratio of the outer covering film to water is 1: 2.
Further, the environment temperature of the inner wrapping film coating in the step (2) is not lower than 10 ℃, and the low-temperature drying treatment temperature is 35 ℃.
Further, the mass ratio of the coating agent in the step (2) to the rice seeds is (1.8-2.2): 1.
Further, the rice seeds pretreated in the step (2) are obtained by the following method:
(1) mixing rape pollen and RO reverse osmosis treated tap water according to a weight ratio of 1:2, heating to 60-70 ℃, stirring at a high speed for 15-20 min, wherein the rpm is 8000-10000 r/min, keeping the constant temperature and standing for 2h after the completion, and filtering to obtain a first filtrate for later use;
(2) pouring shell powder with the particle size of less than 180 mu m into 50 times of 0.1-0.2 wt% sodium hydroxide solution, stirring for 5-10 min at the rpm of 3000-5000 r/min, standing for 24h after the stirring is finished, and filtering to obtain first filter residue for later use;
(3) soaking the rice seeds in a hydrochloric acid solution, and filtering out floating slag from the soaking solution to obtain a second filtrate for later use;
(4) pouring the first filter residue into the second filtrate, stirring for 5-10 min at the rpm of 3000-5000 r/min, standing for 24h after the end, and filtering to obtain a third filtrate for later use;
(5) mixing the first filtrate and the third filtrate according to the mass ratio of 1:50, heating to 30-40 ℃, preserving heat for 24 hours, and filtering after the heating is finished to obtain a fourth filtrate for later use;
(6) and (5) placing the rice seeds which are spread and aired into the fourth filtrate to be soaked for 12 hours, thus obtaining the rice seed soaking agent.
Further, the rice seeds are soaked in hydrochloric acid and the method comprises the following steps:
adding 10 times of 0.4-0.6 wt% hydrochloric acid solution into the rice seeds, soaking the seeds for 24 hours, taking out the seeds after the soaking, washing the seeds for 2 times by using clear water, and spreading and airing for 3-4 hours to obtain the rice seeds.
And (3) further, the water content of the product obtained in the step (3) is lower than 13%, and the product is a qualified finished product.
The invention has the beneficial effects that:
according to the invention, the rice seeds are pretreated by adopting specific process steps, and the rice seeds are soaked by combining the filtrate extracted for multiple times in the treatment process, wherein the components in the first filtrate can effectively promote the growth of substances for germination of the rice seeds. After the components in the fourth filtrate are superposed, the purity of substances which are beneficial to germination in the rice seeds can be higher, and the activity of the rice seeds in a pretreatment stage is ensured.
The invention adopts a special coating agent to coat the pretreated rice seeds, and strictly limits the mass ratio of the coating agent to the rice seeds to 1:1 in the coating process. If the seeds have awns and are large in length and width, the mass ratio of the coating agent to the rice seeds can be properly improved; for awnless round-grained seeds, the mass ratio of the coating agent can be properly reduced. The coating agent prepared by the specific formula can effectively improve the germination rate, germination index and vitality index of rice seeds under the low-oxygen condition and reduce the mildew rate of the rice seeds. Solves the problems of oxygen deficiency caused by overlarge rainfall, water accumulation in the pits on the field surface and overlarge covering soil, and provides reliable guarantee for increasing the income and increasing the yield of the rice.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
Example 1
Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition
(1) Preparing a coating agent:
firstly, adding 1.5 times of water into an inner wrapping film, and uniformly stirring to prepare an inner wrapping film agent for later use; the inner wrapping film includes: 2.4 parts of nutrient reinforcer; 0.3 part of calcium peroxide; and 0.5 parts of tapioca starch resin; the nutrient enhancer is prepared by mixing 2.28 parts of straw powder and 0.12 part of ferment bacteria (Islands, manufacturers are Islands' microbiological research institute);
adding 2 times of water into the outer coating film, and uniformly stirring to prepare the outer coating film agent for later use; the outer coating film includes: 0.9 part of dinotefuran; 5 parts of carbamate; 3 parts of ethylene glycol; and 5.5 parts of a film forming agent; the film forming agent is prepared by mixing 3 parts of polyvinyl alcohol, 2 parts of sodium alginate and 0.5 part of styrene.
(2) Preparation of pretreated rice seeds:
mixing rape pollen and running water subjected to RO reverse osmosis treatment according to a weight ratio of 1:2, heating to 60-70 ℃, stirring at a high speed for 15-20 min at an rpm of 8000-10000 r/min, standing for 2h at a constant temperature after finishing, and filtering to obtain a first filtrate for later use;
pouring the shell powder with the particle size of 80 meshes into 50 times of 0.1-0.2 wt% of sodium hydroxide solution, stirring for 5-10 min at the rpm of 3000-5000 r/min, standing for 24h after the stirring is finished, and filtering to obtain first filter residue for later use;
thirdly, adding 10 times of 0.4-0.6 wt% hydrochloric acid solution into the rice seeds to soak the seeds for 24 hours, taking out the seeds after the soaking for washing for 2 times by using clear water, spreading and airing for 3-4 hours, and filtering floating slag from the soaking solution to obtain a second filtrate for later use;
pouring the first filter residue into the second filtrate, stirring for 5-10 min at the rpm of 3000-5000 r/min, standing for 24h after the end, and filtering to obtain a third filtrate for later use;
fifthly, mixing the first filtrate and the third filtrate according to the mass ratio of 1:50, heating to 30-40 ℃, preserving heat for 24 hours, and filtering after the heating to obtain a fourth filtrate for later use;
sixthly, putting the rice seeds which are spread and dried in the air into the fourth filtrate to be soaked for 12 hours, and obtaining the pretreated rice seeds.
(3) Seed coating treatment:
firstly, mixing the pretreated rice seeds with an inner coating agent, placing the mixture in a seed coating machine for coating, wherein the environmental temperature in the coating process is not lower than 10 ℃, placing the seeds in a cool and dry environment for 2-3 hours after the inner coating film coating is finished,
secondly, mixing the rice seeds coated by the inner coating agent with the outer coating agent, placing the mixture into a seed coating machine for coating, placing the coated rice seeds into a low-temperature dryer, and drying the rice seeds at the temperature below 35 ℃;
testing the moisture content of the dried coated rice seeds, and if the moisture content is lower than 13%, obtaining a qualified finished product, and storing the finished product in a waterproof package.
When coating, the mass ratio of the coating agent to the rice seeds is (1.8-2.2): 1. If the seeds have awns and are large in length and width, the mass ratio of the coating agent to the rice seeds can be properly improved; for awnless round-grained seeds, the mass ratio of the coating agent can be properly reduced. If the adding amount of the coating agent is too large, the cost of the agent is increased, and if the adding amount of the coating agent is too small, the coating is not uniform, and the coating quality is influenced.
Example 2
Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition
(1) Preparing a coating agent:
firstly, adding 1.5 times of water into an inner wrapping film, and uniformly stirring to prepare an inner wrapping film agent for later use; the inner wrapping film includes: 2.4 parts of nutrient reinforcer; 0.2 part of calcium peroxide; and 0.35 parts of tapioca starch resin; the nutrient enhancer is prepared by mixing 2.28 parts of straw powder and 0.12 part of ferment bacteria (Islands, manufacturers are Islands' microbiological research institute);
adding 2 times of water into the outer coating film, and uniformly stirring to prepare the outer coating film agent for later use; the outer coating film includes: 0.5 part of dinotefuran; 2 parts of carbamate; 2 parts of ethylene glycol; and 3.1 parts of a film-forming agent; the film forming agent is prepared by mixing 2 parts of polyvinyl alcohol, 1 part of sodium alginate and 0.1 part of styrene.
(2) Preparation of pretreated rice seeds:
mixing rape pollen and running water subjected to RO reverse osmosis treatment according to a weight ratio of 1:2, heating to 60-70 ℃, stirring at a high speed for 15-20 min at an rpm of 8000-10000 r/min, standing for 2h at a constant temperature after finishing, and filtering to obtain a first filtrate for later use;
pouring the shell powder with the particle size of 80 meshes into 50 times of 0.1-0.2 wt% of sodium hydroxide solution, stirring for 5-10 min at the rpm of 3000-5000 r/min, standing for 24h after the stirring is finished, and filtering to obtain first filter residue for later use;
thirdly, adding 10 times of 0.4-0.6 wt% hydrochloric acid solution into the rice seeds to soak the seeds for 24 hours, taking out the seeds after the soaking for washing for 2 times by using clear water, spreading and airing for 3-4 hours, and filtering floating slag from the soaking solution to obtain a second filtrate for later use;
pouring the first filter residue into the second filtrate, stirring for 5-10 min at the rpm of 3000-5000 r/min, standing for 24h after the end, and filtering to obtain a third filtrate for later use;
fifthly, mixing the first filtrate and the third filtrate according to the mass ratio of 1:50, heating to 30-40 ℃, preserving heat for 24 hours, and filtering after the heating to obtain a fourth filtrate for later use;
sixthly, putting the rice seeds which are spread and dried in the air into the fourth filtrate to be soaked for 12 hours, and obtaining the pretreated rice seeds.
(3) Seed coating treatment:
firstly, mixing the pretreated rice seeds with an inner coating agent, placing the mixture in a seed coating machine for coating, wherein the environmental temperature in the coating process is not lower than 10 ℃, placing the seeds in a cool and dry environment for 2-3 hours after the inner coating film coating is finished,
secondly, mixing the rice seeds coated by the inner coating agent with the outer coating agent, placing the mixture into a seed coating machine for coating, placing the coated rice seeds into a low-temperature dryer, and drying the rice seeds at the temperature below 35 ℃;
testing the moisture content of the dried coated rice seeds, and if the moisture content is lower than 13%, obtaining a qualified finished product, and storing the finished product in a waterproof package.
When coating, the mass ratio of the coating agent to the rice seeds is (1.8-2.2): 1. If the seeds have awns and are large in length and width, the mass ratio of the coating agent to the rice seeds can be properly improved; for awnless round-grained seeds, the mass ratio of the coating agent can be properly reduced. If the adding amount of the coating agent is too large, the cost of the agent is increased, and if the adding amount of the coating agent is too small, the coating is not uniform, and the coating quality is influenced.
Comparative example 1
Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition
Comparative example 1 the same procedure as in example 1 was followed except that only the disinfection treatment with a hydrochloric acid solution was maintained during the pretreatment of rice seeds, namely:
adding 10 times of 0.4-0.6 wt% hydrochloric acid solution into the rice seeds, soaking the seeds for 24 hours, taking out the seeds after the soaking, washing the seeds for 2 times by using clear water, and spreading and airing for 3-4 hours.
Comparative example 2
Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition
Comparative example 2 the same procedure as in example 2 was followed except that only the disinfection treatment with the hydrochloric acid solution was maintained during the pretreatment of rice seeds, namely:
adding 10 times of 0.4-0.6 wt% hydrochloric acid solution into the rice seeds, soaking the seeds for 24 hours, taking out the seeds after the soaking, washing the seeds for 2 times by using clear water, and spreading and airing for 3-4 hours.
Comparative example 3
Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition
Comparative example 3 the same pretreatment of seeds as in example 1 was carried out, and a commercially available Bayer income field product coating agent was used as the coating agent.
Comparative example 4
Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition
Comparative example 4 the pretreatment of the seeds was only carried out with the hydrochloric acid solution for sterilization, and the coating agent was a commercially available bayer income field product coating agent.
Adding 10 times of 0.4-0.6 wt% hydrochloric acid solution into the rice seeds, soaking the seeds for 24 hours, taking out the seeds after the soaking, washing the seeds for 2 times by using clear water, and spreading and airing for 3-4 hours.
Statistical observation of the status of each group of rice seeds
(1) And (3) counting the mildew rate: 600 full rice seeds are selected and averagely divided into 6 groups, and the treatments are respectively carried out in the above examples 1-2 and comparative examples 1-4. Storing the coated seeds at the temperature of 25 +/-3 ℃ and the humidity of 50 +/-10%, judging that the seeds are mildewed according to yellow or white moulds appearing on the surfaces of the seeds, and counting the mildewing rate of the seeds after 14 days.
(2) And (3) counting the germination rate, the germination index and the vitality index: 600 full rice seeds were selected and evenly divided into 6 groups, and the above examples 1 to 4 and comparative examples 1 and 2 were performed, respectively. The seeds after coating are placed in a germination box with the size of 50cm multiplied by 20cm, and 2 layers of special germination paper are filled in the germination box. Adding 5cm of deep distilled water into the box, uniformly placing the seeds on germinating paper, and culturing at constant temperature of 30 + -1 deg.C with the water depth of 5 + -0.5 cm. Placing in an incubator, recording the number of sprouts (normal number of seedlings) every day as 0d, counting the number of sprouts till 7 days, cutting off the seedlings, drying and weighing. Germination rate, Germination Index (GI) and Vigor Index (VI) were calculated according to the following formulas:
the germination number is the number of normal seedlings/tested seeds multiplied by 100%
The germination potential is equal to the number of normal seedlings/tested seeds in the first 3 days, and is multiplied by 100 percent
GI＝∑(Gt/Dt)
VI＝GI×S
In the formula, Dt is the number of germination days, and Gt is the number of germination seeds on the day corresponding to Dt; s is the dry weight of the seedling in g in a certain period (7 d).
The statistical results of the above experiments are shown in table 1.
TABLE 1 germination of seeds treated with different coatings
| Examples/comparative examples | Mold growth rate (%) | Percentage of germination (%) | Germination vigor (%) | Index of germination | Vitality index |
| Example 1 | 1.45 | 76.45 | 74.34 | 36.32 | 8.82 |
| Example 2 | 1.67 | 78.32 | 79.23 | 39.05 | 9.03 |
| Comparative example 1 | 1.76 | 63.21 | 60.30 | 30.32 | 7.42 |
| Comparative example 2 | 2.02 | 61.03 | 62.23 | 31.67 | 7.24 |
| Comparative example 3 | 1.69 | 62.29 | 60.04 | 28.87 | 7.82 |
| Comparative example 4 | 1.92 | 58.12 | 51.18 | 22.67 | 6.24 |
According to the data in table 1, the coating agents prepared in the examples 1 and 2 according to the present invention can effectively reduce the mildew rate of rice seeds and improve the germination rate and germination vigor of rice seeds by coating the pretreated rice seeds, and the germination index and vitality index of the seeds are also significantly higher than those of the other four control groups.
Comparative example 1 the same procedure as in example 1 was conducted except that only the sterilization treatment with a hydrochloric acid solution was maintained during the pretreatment of rice seeds. Comparative example 2 the same procedure as in example 2 was followed except that only the disinfection treatment with a hydrochloric acid solution was maintained during the pretreatment of rice seeds. Comparative example 3 the same procedure as in example 1 was conducted except that a commercially available Bayer Process product coating agent was used for coating rice seeds. Comparative example 4 the same procedure as in example 1 was conducted except that a commercially available Bayer Process product coating agent was used in the coating treatment of rice seeds. Meanwhile, only the hydrochloric acid solution is reserved for disinfection treatment in the rice seed pretreatment process. The comparison between the example 1 and the comparative example 1 and the comparison between the example 2 and the comparative example 2 show that the seed pretreatment method provided by the invention can reduce the mildew rate of the seeds compared with the method only adopting hydrochloric acid for disinfection, and the germination rate, the germination vigor, the germination index and the vitality index of the seeds under the flooding condition are better. Comparison of examples 1 and 2 with comparative example 3 and comparison of comparative examples 1 and 2 with comparative example 4 shows that the germination rate, germination vigor, germination index and vigor index of seeds under flooding conditions of the coating agent are better than those of the commercial products compared with the commercial representative products. Therefore, the combination of the seed pretreatment method and the coating agent can obviously improve the germination rate of the direct-seeded rice seeds under the low-oxygen condition.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. A rice seed coating agent comprising:
an inner wrapping film and an outer covering film;
wherein the inner wrapping film comprises: 2.3-3.8 parts of a nutrient enhancer; 0.2-0.5 parts of an oxygen increasing agent; and 0.35-0.80 parts of water-retaining agent;
the outer coating film includes: 0.5-1.3 parts of an insecticide; 2-7 parts of a bactericide; 2-6 parts of an antifreezing agent; and 1-6 parts of a film forming agent.
2. The coating agent according to claim 1, wherein:
the nutrient enhancer is prepared by mixing straw powder and enzyme bacteria in a mass ratio of (85-95) to (5-15);
the oxygen increasing agent is calcium peroxide, and the water-retaining agent is cassava starch resin.
3. The coating agent according to claim 1, wherein:
the pesticide is dinotefuran, the bactericide is carbamate, and the antifreezing agent is ethylene glycol;
the film forming agent is prepared by mixing polyvinyl alcohol, sodium alginate and styrene according to the mass ratio of (2-5) to (1-2) to (0.1-0.5).
4. Use of the direct-seeded rice seed coating agent according to any one of claims 1 to 3 for increasing the germination rate of direct-seeded rice seeds under low-oxygen conditions, comprising:
(1) respectively adding water into the inner coating film and the outer coating film and uniformly stirring to obtain an inner coating agent and an outer coating film agent for later use;
(2) mixing and coating the pretreated rice seeds and the inner coating agent, naturally placing for 2-3 hours, mixing and coating the rice seeds and the outer coating agent after finishing, and placing the rice seeds and the outer coating agent in a low-temperature dryer for drying treatment after finishing;
(3) measuring the water content of the dried coated rice seeds;
(4) and (5) waterproof packaging and storing the qualified seeds.
5. The use of claim 4, wherein:
the mass ratio of the inner wrapping film to water in the step (1) is 1:1.5, and the mass ratio of the outer wrapping film to water is 1: 2.
6. The use of claim 4, wherein:
the environment temperature of the inner wrapping film coating in the step (2) is not lower than 10 ℃, and the low-temperature drying treatment temperature is 35 ℃.
7. The use of claim 4, wherein:
the mass ratio of the coating agent in the step (2) to the rice seeds is (1.8-2.2): 1.
8. The use of claim 4, wherein:
the rice seeds pretreated in the step (2) are obtained by the following method:
(1) mixing rape pollen and RO reverse osmosis treated tap water according to a weight ratio of 1:2, heating to 60-70 ℃, stirring at a high speed for 15-20 min, wherein the rpm is 8000-10000 r/min, keeping the constant temperature and standing for 2h after the completion, and filtering to obtain a first filtrate for later use;
(2) pouring shell powder with the particle size of less than 180 mu m into 50 times of 0.1-0.2 wt% sodium hydroxide solution, stirring for 5-10 min at the rpm of 3000-5000 r/min, standing for 24h after the stirring is finished, and filtering to obtain first filter residue for later use;
(3) soaking the rice seeds in a hydrochloric acid solution, and filtering out floating slag from the soaking solution to obtain a second filtrate for later use;
(4) pouring the first filter residue into the second filtrate, stirring for 5-10 min at the rpm of 3000-5000 r/min, standing for 24h after the end, and filtering to obtain a third filtrate for later use;
(5) mixing the first filtrate and the third filtrate according to the mass ratio of 1:50, heating to 30-40 ℃, preserving heat for 24 hours, and filtering after the heating is finished to obtain a fourth filtrate for later use;
(6) and (5) placing the rice seeds which are spread and aired into the fourth filtrate to be soaked for 12 hours, thus obtaining the rice seed soaking agent.
9. The use of claim 8, wherein:
the rice seeds are soaked by hydrochloric acid and the method comprises the following steps:
adding 10 times of 0.4-0.6 wt% hydrochloric acid solution into the rice seeds, soaking the seeds for 24 hours, taking out the seeds after the soaking, washing the seeds for 2 times by using clear water, and spreading and airing for 3-4 hours to obtain the rice seeds.
10. The use of claim 3, wherein:
and (4) obtaining a qualified finished product when the water content is lower than 13% in the step (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111440145.XA CN114375642A (en) | 2021-11-29 | 2021-11-29 | Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111440145.XA CN114375642A (en) | 2021-11-29 | 2021-11-29 | Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114375642A true CN114375642A (en) | 2022-04-22 |
Family
ID=81195060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111440145.XA Pending CN114375642A (en) | 2021-11-29 | 2021-11-29 | Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114375642A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314954A (en) * | 2013-07-04 | 2013-09-25 | 江苏省绿盾植保农药实验有限公司 | Seed coating agent for preventing rice sheath blight diseases, preparing method and application method thereof |
| CN103787769A (en) * | 2014-01-26 | 2014-05-14 | 微港生物科技江苏有限公司 | Special calcium fertilizer for paddy, preparation of special calcium fertilizer and method for producing calcium-rich rice |
| CN105612864A (en) * | 2016-01-28 | 2016-06-01 | 华中农业大学 | Seed pelleting method for promoting one-time seeding of full seedlings under wet direct seeding paddy flooding conditions |
| CN105660719A (en) * | 2016-01-11 | 2016-06-15 | 中国水稻研究所 | Oxygen-evolving seed coating agent for promoting direct seeding rice seed germination and seedling development and coating method |
| CN106612728A (en) * | 2016-11-29 | 2017-05-10 | 颍上县锡安山水稻种植专业合作社 | Method for treating rice seeds |
| CN108640762A (en) * | 2018-07-19 | 2018-10-12 | 山东祥维斯生物科技股份有限公司 | Composite regulator for the conditioning of rice in saline-alkali field seedling medium and its preparation |
| CN108934851A (en) * | 2018-07-19 | 2018-12-07 | 山东祥维斯生物科技股份有限公司 | A kind of saline-alkali tolerant degeneration-resistant paddy seedling culture method coldly |
| CN110558180A (en) * | 2019-09-26 | 2019-12-13 | 贵州黔西南喀斯特区域发展研究院 | Planting method for high-yield rice in karst region |
| CN111109029A (en) * | 2020-02-14 | 2020-05-08 | 段秀梅 | High-calcium rice planting method |
| CN111903715A (en) * | 2020-08-17 | 2020-11-10 | 江西农业大学 | Direct-seeding rice cold-resistant and flood-resistant seed coating agent and preparation method and use method thereof |
| AU2020103185A4 (en) * | 2020-11-02 | 2021-01-14 | Sichuan Agricultural University | Rice seed coating agent and coating method |
-
2021
- 2021-11-29 CN CN202111440145.XA patent/CN114375642A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314954A (en) * | 2013-07-04 | 2013-09-25 | 江苏省绿盾植保农药实验有限公司 | Seed coating agent for preventing rice sheath blight diseases, preparing method and application method thereof |
| CN103787769A (en) * | 2014-01-26 | 2014-05-14 | 微港生物科技江苏有限公司 | Special calcium fertilizer for paddy, preparation of special calcium fertilizer and method for producing calcium-rich rice |
| CN105660719A (en) * | 2016-01-11 | 2016-06-15 | 中国水稻研究所 | Oxygen-evolving seed coating agent for promoting direct seeding rice seed germination and seedling development and coating method |
| CN105612864A (en) * | 2016-01-28 | 2016-06-01 | 华中农业大学 | Seed pelleting method for promoting one-time seeding of full seedlings under wet direct seeding paddy flooding conditions |
| CN106612728A (en) * | 2016-11-29 | 2017-05-10 | 颍上县锡安山水稻种植专业合作社 | Method for treating rice seeds |
| CN108640762A (en) * | 2018-07-19 | 2018-10-12 | 山东祥维斯生物科技股份有限公司 | Composite regulator for the conditioning of rice in saline-alkali field seedling medium and its preparation |
| CN108934851A (en) * | 2018-07-19 | 2018-12-07 | 山东祥维斯生物科技股份有限公司 | A kind of saline-alkali tolerant degeneration-resistant paddy seedling culture method coldly |
| CN110558180A (en) * | 2019-09-26 | 2019-12-13 | 贵州黔西南喀斯特区域发展研究院 | Planting method for high-yield rice in karst region |
| CN111109029A (en) * | 2020-02-14 | 2020-05-08 | 段秀梅 | High-calcium rice planting method |
| CN111903715A (en) * | 2020-08-17 | 2020-11-10 | 江西农业大学 | Direct-seeding rice cold-resistant and flood-resistant seed coating agent and preparation method and use method thereof |
| AU2020103185A4 (en) * | 2020-11-02 | 2021-01-14 | Sichuan Agricultural University | Rice seed coating agent and coating method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2020103168A4 (en) | Method for planting berry seeds | |
| CN105638025A (en) | Anthocephalus chinensiss seedling culture method | |
| CN103858771B (en) | A kind of transplanting method of corn gene plantlet in vitro | |
| CN101828446B (en) | Cold-humid adversity resisting tobacco coated seed and preparation method thereof | |
| CN111280003A (en) | Asparagus cochinchinensis seed breeding method | |
| JP7454587B2 (en) | Plant growth regulators and their applications | |
| CN107494230A (en) | A kind of spring method for culturing seedlings of Acer palmatum seed | |
| CN107155782A (en) | A kind of sweet osmanthus cuttage and seedling culture method | |
| CN111492905A (en) | Sowing method of summer squash | |
| CN101268734B (en) | Root-protecting seedling raising cultivation method | |
| CN114375642A (en) | Method for improving germination rate of direct-seeded rice seeds under low-oxygen condition | |
| CN101828443A (en) | Solid matrix initiating method for improving germination rate and vitality of watermelon seeds | |
| CN113142060B (en) | Method for rapid and efficient propagation of Japanese beautyberry | |
| CN115093975A (en) | Arbuscular mycorrhizal fungi microbial inoculum and preparation method and application thereof | |
| CN114128713A (en) | Plateau elymus nutans seed coating agent and using method thereof | |
| CN113498724A (en) | Seedling growing method for tropical water lily | |
| CN110946054A (en) | Tea tree culture medium and preparation method thereof | |
| CN114451096B (en) | Efficient wild jujube seedling cultivation method | |
| CN107810801A (en) | A kind of high yield eggplant implantation methods | |
| CN108094453A (en) | A kind of paddy rice seed soaking agent and its preparation method and application | |
| CN113439656B (en) | Microbial inoculation and wheat breeding method | |
| CN107873460A (en) | A kind of method for improving silver-colored side bracketplant transplanting survival rate | |
| CN116034667A (en) | Cultivation method of space-induced konjak seed | |
| CN112219648A (en) | Strawberry seedling culture method | |
| CN114982560A (en) | Method for raising seedlings by utilizing sunflower young seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220422 |
|
| RJ01 | Rejection of invention patent application after publication |