CN108094453A - A kind of paddy rice seed soaking agent and its preparation method and application - Google Patents
A kind of paddy rice seed soaking agent and its preparation method and application Download PDFInfo
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- CN108094453A CN108094453A CN201810057657.XA CN201810057657A CN108094453A CN 108094453 A CN108094453 A CN 108094453A CN 201810057657 A CN201810057657 A CN 201810057657A CN 108094453 A CN108094453 A CN 108094453A
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- seed soaking
- soaking agent
- paddy rice
- rice seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
Abstract
The present invention provides a kind of paddy rice seed soaking agents and its preparation method and application, belong to field of agricultural microbial technology.Seed soaking agent is made using natural material, is had no adverse effects to human body and vegetable seeds, while the seedling germination percentage after using is high, and Resistance of Seedling is strong, and seedling growth is good.Seed soaking agent can be degradable under microorganism species effect in the soil, and unharmful substance residual, the seed for realizing green high-efficient inspires bud, growth-promoting effect.The preparation method of seed soaking agent is by rational parameter setting, and raw material is simple and easy to get, can largely prepare efficient paddy rice seed soaking agent, above-mentioned paddy rice seed soaking agent is applied in Rice Cropping, can obtain preferable germination percentage and growth result.
Description
Technical field
The present invention relates to field of agricultural microbial technology, in particular to a kind of paddy rice seed soaking agent and preparation method thereof
And application.
Background technology
With the development of agricultural, the fast seedling growing of seed becomes an important act for improving Agriculture, forestry And Animal Husbandry yield of commercial crops
Arrange, and seed because external environment be not suitable for or own growth is immature, cause seed dormancy phase is long, germination percentage is low, budding not
The situations such as neat and germination quality is low, have seriously affected the growth of later stage crop, and result in that the production cycle is long, product quality
A series of problems, such as not high, influences economic benefit.Further, since seeds self carry disease germs and soil in phytopathogen generally deposit
The phenomenon that plant seedling stage disease is caused to occur, is also more universal.The plantation of rice in north china is using after elder generation's nursery by the way of rice transplanting.
In the northern area of China, ground rice region of especially trembling with fear, rice paddy seed is temperature sensitive since germinating time is long, and potential difference of germinateing often goes out
The inconsistent situation of existing rice shoot growing way.It is bakanae disease during raising rice seedlings, vertical further, since rice paddy seed and seedbed soil carry disease germs
The diseases such as blight happen occasionally.South rice plantation meets the variation of the weather conditions such as cold spell in later spring mostly by the way of live streaming,
Building up for seedling can be impacted.
The content of the invention
The first object of the present invention is to provide a kind of paddy rice seed soaking agent, which has preferable selection and sieve
Select the effect of culture.
The second object of the present invention is to provide a kind of preparation method of paddy rice seed soaking agent, by the way that a process for preparing favourable
In the seed soaking agent of Seed Germination of Rice.
The third object of the present invention is in the application in the above-mentioned paddy rice seed soaking agent of offer in Rice Cropping.
In order to realize the above-mentioned purpose of the present invention, using following technical scheme：
A kind of paddy rice seed soaking agent, paddy rice seed soaking agent include S. ahygroscopicus Gongzhuling new variant and corn quarrel matrix.
A kind of preparation method of paddy rice seed soaking agent, comprises the following steps：
The quarrel matrix of the corn by immersion that grain size is 0.5-0.7cm is subjected to sterilization treatment；
S. ahygroscopicus Gongzhuling new variant of the inoculation Jing Guo activation culture is cultivated after cooling, obtains solid culture
Object；
Dry and comminuted solids culture, obtains culture powder, with flooding culture powder, collected 100-200
Paddy rice seed soaking agent is made in water extract by the water extract of mesh sieve.
Application of the above-mentioned paddy rice seed soaking agent in Rice Cropping.
Compared with prior art, beneficial effects of the present invention are：Seed soaking agent is made using natural material, to human body and plant
Seed has no adverse effects, while the seedling germination percentage after using is high, and Resistance of Seedling is strong, and seedling growth is good.Seed soaking agent can be
Degradable under the effect of Soil Microorganism flora, unharmful substance residual, the seed for realizing green high-efficient inspires bud, growth-promoting
Long effect.The preparation method of seed soaking agent is by rational parameter setting, and raw material is simple and easy to get, can largely prepare high efficiency
Paddy rice seed soaking agent, above-mentioned paddy rice seed soaking agent is applied in Rice Cropping, preferable germination percentage and growth result can be obtained.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person, the condition suggested according to normal condition or manufacturer carry out.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
A kind of paddy rice seed soaking agent of the embodiment of the present invention and its preparation method and application is specifically described below.
A kind of paddy rice seed soaking agent, paddy rice seed soaking agent is mainly by S. ahygroscopicus Gongzhuling new variant and corn quarrel matrix.
Further, in preferred embodiments of the present invention, the grain size of corn quarrel matrix is 0.5-0.7cm.
The corn quarrel matrix of 0.5-0.7cm is selected, can provide and fill for the growth of S. ahygroscopicus Gongzhuling new variant
The space of foot and throughput.The processing equipment of 0.5-0.7cm grain sizes is ripe, and processing cost is relatively low.
The fibrillae of spores long spire shape of S. ahygroscopicus Gongzhuling new variant, minimum 3 circle, general 5-8 circles, up to 12 circles,
Pine is spacious or close.Spore ellipse, lemon shape or semilune, the slightly longer spinelet of surface band.In sucrose nitrate agar medium
Gas silk litchi meat white；Base silk is light yellow；It can not lysochrome in synthetic agar；The shallow pine soot ash of gas silk in glucose asparagus fern element agar
Color, monkey hair grey, local canescence, suede powdery.Base silk ivory yellow, sail yellow.
Corn quarrel matrix provides the nutriment needed for growth, amount reproduction, the metabolism production of microorganism secretion for microorganism
Object is conducive to the sprouting of seed.
A kind of preparation method of paddy rice seed soaking agent, comprises the following steps：
The quarrel matrix of the corn by immersion that grain size is 0.5-0.7cm is subjected to sterilization treatment；
S. ahygroscopicus Gongzhuling new variant of the inoculation Jing Guo activation culture is cultivated after cooling, obtains solid culture
Object；
Dry and comminuted solids culture, obtains culture powder, with flooding culture powder, collected 100-200
Paddy rice seed soaking agent is made in water extract by the water extract of mesh sieve.
Further, in preferred embodiments of the present invention, the time of the immersion of corn quarrel matrix is 2h-4h.
Further, in preferred embodiments of the present invention, sterilization treatment is high pressure steam sterilization, high pressure steam sterilization
Temperature is 121 DEG C, time 20min.
Further, in preferred embodiments of the present invention, the temperature strip of S. ahygroscopicus Gongzhuling new variant culture
Part is 24.3 DEG C -30.6 DEG C, and the time is 5-7 days.
Further, in preferred embodiments of the present invention, extraction uses the solid-liquid ratio of culture powder and water as 1:2-4
It carries out.
Application of the above-mentioned screening and culturing medium in microbe to screen culture.
Further, in preferred embodiments of the present invention, paddy rice seed soaking agent is diluted to seed soaking agent dilution, by rice
Seed is impregnated 3-5 days with seed soaking agent dilution, and 20-30 DEG C of soaking temperature, accumulated temperature are 100 DEG C/day, pull out to drain and move to 28-33
The germination of DEG C seedbed.
Further, diluted multiple is 100-1000 times.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of paddy rice seed soaking agents.
The paddy rice seed soaking agent includes S. ahygroscopicus Gongzhuling new variant and corn quarrel matrix, wherein corn quarrel matrix
Grain size is 0.5cm.
The present embodiment also provides a kind of preparation method of paddy rice seed soaking agent；Specific preparation method comprises the following steps：
The corn quarrel matrix clear water that grain size is 0.5cm is impregnated 110min by 1.1；
1.2 taking-up corn quarrel matrix drain, and sterilization treatment is carried out using high pressure steam sterilization method；121 DEG C of sterilising temp,
Sterilization time 20min；
The strain of S. ahygroscopicus Gongzhuling new variant is inoculated in culture plate by 1.3 to be cultivated；
1.4 after bacterial plaque grows up to, and takes 1 square centimeter or so of fungus block 4, be inoculated in 300 cubic centimetres it is sterilized
Corn quarrel matrix on；
1.5 cultivate 5 days under 26.3 DEG C of temperature conditionss, obtain solid culture；
1.6 drying solid culture and crush under normal temperature condition, obtain culture powder；
1.7 by obtained culture powder and according to 1:2 solid-liquid ratio is extracted；
The water extract of 100 mesh sieves was collected after 1.8 extractions, paddy rice seed soaking agent is made in water extract.
After paddy rice seed soaking agent is made, paddy rice seed soaking agent can be applied in Seed Germination of Rice, be conducive to seed rice sprouting and
Seedling establishment.
The present embodiment also provides a kind of application of above-mentioned paddy rice seed soaking agent in Rice Cropping, including applying in detail below
Step：
2.1 100 times of the rice seed soaking dilution agents that will be obtained in step 1.8, are diluted to seed soaking agent dilution；
2.2 impregnate rice paddy seed seed soaking agent dilution, are totally submerged seed；
2.3 impregnated 5 days under 20 DEG C of temperature conditionss after pull seed out and drain；
It germinates in 2.4 seedbeds that the seed impregnated is moved to 28 DEG C；
2.5 maintain normal germination condition to sowing during germination.
Embodiment 2
The present embodiment provides a kind of paddy rice seed soaking agents.
The paddy rice seed soaking agent includes S. ahygroscopicus Gongzhuling new variant and corn quarrel matrix, wherein corn quarrel matrix
Grain size is 0.7cm.
The present embodiment also provides a kind of preparation method of paddy rice seed soaking agent；Specific preparation method comprises the following steps：
The corn quarrel matrix clear water that grain size is 0.7cm is impregnated 135min by 1.1；
1.2 taking-up corn quarrel matrix drain, and sterilization treatment is carried out using high pressure steam sterilization method；121 DEG C of sterilising temp,
Sterilization time 20min；
The strain of S. ahygroscopicus Gongzhuling new variant is inoculated in culture plate by 1.3 to be cultivated；
1.4 after bacterial plaque grows up to, and takes 1 square centimeter or so of fungus block 8, be inoculated in 300 cubic centimetres it is sterilized
Corn quarrel matrix on；
1.5 cultivate 7 days under 30.6 DEG C of temperature conditionss, obtain solid culture；
1.6 drying solid culture and crush under normal temperature condition, obtain culture powder；
Obtained culture is powder and according to 1 by 1.7:4 solid-liquid ratio is extracted；
The water extract of 200 mesh sieves was collected after 1.8 extractions, paddy rice seed soaking agent is made in water extract.
After paddy rice seed soaking agent is made, paddy rice seed soaking agent can be applied in Seed Germination of Rice, be conducive to the sprouting of rice.
The present embodiment also provides a kind of application of paddy rice seed soaking agent stated in Rice Cropping, including application step in detail below
Suddenly：
2.1 1000 times of the rice seed soaking dilution agents that will be obtained in step 1.8, are diluted to seed soaking agent dilution；
2.2 impregnate rice paddy seed seed soaking agent dilution, are totally submerged seed；
2.3 impregnated 3 days under 30 DEG C of temperature conditionss after pull seed out and drain；
It germinates in 2.4 seedbeds that the seed impregnated is moved to 33 DEG C；
2.5 maintain normal germination condition to sowing during germination.
Embodiment 3
The present embodiment provides a kind of paddy rice seed soaking agents.
The paddy rice seed soaking agent includes S. ahygroscopicus Gongzhuling new variant and corn quarrel matrix, wherein corn quarrel matrix
Grain size is 0.6cm.
The present embodiment also provides a kind of preparation method of paddy rice seed soaking agent；Specific preparation method comprises the following steps：
The corn quarrel matrix clear water that grain size is 0.6cm is impregnated 120min by 1.1；
1.2 taking-up corn quarrel matrix drain, and sterilization treatment is carried out using high pressure steam sterilization method；121 DEG C of sterilising temp,
Sterilization time 20min；
The strain of S. ahygroscopicus Gongzhuling new variant is inoculated in culture plate by 1.3 to be cultivated；
1.4 after bacterial plaque grows up to, and takes 1 square centimeter or so of fungus block 6, be inoculated in 300 cubic centimetres it is sterilized
Corn quarrel matrix on；
1.5 cultivate 6 days under 28 DEG C of temperature conditionss, obtain solid culture；
1.6 drying solid culture and crush under normal temperature condition, obtain culture powder；
Obtained culture is powder and according to 1 by 1.7:3 solid-liquid ratio is extracted；
The water extract of 150 mesh sieves was collected after 1.8 extractions, paddy rice seed soaking agent is made in water extract.
After paddy rice seed soaking agent is made, paddy rice seed soaking agent can be applied in Seed Germination of Rice, be conducive to the sprouting of rice.
The present embodiment also provides a kind of application of paddy rice seed soaking agent stated in Rice Cropping, including application step in detail below
Suddenly：
2.1 500 times of the rice seed soaking dilution agents that will be obtained in step 1.8, are diluted to seed soaking agent dilution；
2.2 impregnate rice paddy seed seed soaking agent dilution, are totally submerged seed；
2.3 impregnated 4 days under 25 DEG C of temperature conditionss after pull seed out and drain；
It germinates in 2.4 seedbeds that the seed impregnated is moved to 30 DEG C；
2.5 maintain normal germination condition to sowing during germination.
Experimental example 1
This experimental example provides the different dilution ratio adding proportions of paddy rice seed soaking agent prepared by embodiment 3 to rice seedling rate
Influence.
Target crops are chosen rice Ji round-grained rice 88 and are carried out in embodiment, and method of administration is broadcast live using seed soaking；In incubator culture
(25 DEG C) are broadcast live in ware, specific germinating method reference implementation example 3.Specific dilution ratio and emergence situation are shown in Table 1.
The emergence rate situation of 1 rice of table
| Dilution ratio | Seed number | Number of emerging | Emergence rate % | Relative seedling ratio % |
| ck | 50 | 26 | 52 | 100.00 |
| 1:50 | 50 | 27 | 54 | 103.85 |
| 1:70 | 50 | 34 | 68 | 130.77 |
| 1:90 | 50 | 34 | 68 | 130.77 |
| 1:100 | 50 | 35 | 70 | 134.62 |
| 1:200 | 50 | 31 | 62 | 119.23 |
| 1:300 | 50 | 30 | 60 | 115.38 |
| 1:800 | 50 | 35 | 70 | 114.62 |
| 1:1000 | 50 | 28 | 56 | 107.69 |
As it can be seen from table 1 using control group CK as benchmark, it is found that when sowing, soaked with paddy rice seed soaking agent
After kind, emergence rate has different degrees of raising；In terms of 50 times to 1000 times of result of dilution, substantially decline afterwards into first rising
Trend, wherein most suitable extension rate be 70-100 times.
Experimental example 2
The seed soaking agent Artest spirit of paddy rice seed soaking agent and conventional commercial that this experimental example is provided by using embodiment 3 is to rice
The influence of growth of seedling.
Target crops are chosen rice Ji round-grained rice 88 and are carried out in embodiment, and method of administration is broadcast live using seed soaking；In outdoor seedlings nursing plate
In carry out growth 64 days, specific germinating method reference implementation example 3.Measure the physiological parameters such as plant height, the thick root long of stem of seedling.Specifically
Result of the test be shown in Table 2.
Influence of 2 seed soaking agent of table to young rice seedlings growth
From table 2 it can be seen that after using seed soaking agent, the growth of rice seedling can be promoted, plant height is above blank control CK
Group, paddy rice seed soaking agent especially provided by the invention is in the case of 100 times of dilution, plant height highest；Facilitation is apparent.Equally
Paddy rice seed soaking agent provided by the invention is long to rice main root, fresh weight and dry weight slightly have facilitation；And conventional seed soaking agent is bright
The parameters such as the aobvious fresh weight for inhibiting rice and dry weight.
Experimental example 3
This experimental example is by verifying that the different extension rate of paddy rice seed soaking agent prepared by embodiment 3 acts on different growth times
The interior influence to rice seedling plant height；And the comparison with Normal seed coating seedling shield.
Target crops are chosen rice dragon round-grained rice 44 and are carried out in embodiment, and method of administration is broadcast live using seed soaking；The nursery in greenhouse,
25 DEG C of temperature, specific germinating method reference implementation example 3.Specific result of the test is shown in Table 3.
3 seed soaking agent of table difference action time influences rice seedling plant height
| Processing | 7 days | 14 days | 24 days |
| Paddy rice seed soaking agent 200X | 8.073 | 9.89 | 13.4075 |
| Paddy rice seed soaking agent 500X | 7.9465 | 9.7635 | 13.7825 |
| Paddy rice seed soaking agent 1000X | 7.3715 | 8.8435 | 13.5875 |
| Seedling shield | 7.469 | 9.5565 | 12.025 |
| CK | 6.693 | 8.9355 | 12.025 |
From table 3 it can be seen that in the case of growing 7 days, paddy rice seed soaking agent provided in an embodiment of the present invention and conventional kind of clothing
The bright shield of agent can promote the growth of rice seedling plant height, and the facilitation of 200 times and 500 times of dilution is most apparent；When growing into 14
My god, the paddy rice seed soaking agent of 10 times of dilution shows a degree of inhibition；When growing into 24 days, rice provided by the invention
Seed soaking agent is in the case of 200 times, 500 times and 1000 times of dilution, hence it is evident that promote paddy growth, and better than Normal seed coating and right
According to group.
Experimental example 4
This experimental example goes out the rice of sowing in the field after providing verification paddy rice seed soaking agent prepared by the embodiment of the present invention 3 effect
The influence of seedling rate；And compared with the seed coat agent of conventional commercial.
Target crops choose rice and praise colored No.1 progress in embodiment, and method of administration is broadcast live using seed soaking；It is straight using crop field
It broadcasts, specific germinating method reference implementation example 3.Specific result of the test is shown in Table 4.
4 sowing in the field rice seedling rate of table
From table 4, it can be seen that paddy rice seed soaking agent provided by the invention has no adverse effect the emergence rate of rice, have smaller
Facilitation；And the emergence rate that the seed coat agent of conventional commercial plants rice field has a degree of inhibitory action；It can be with
Find out, paddy rice seed soaking agent provided by the invention is no inhibitory action to the emergence rate of rice.
In conclusion paddy rice seed soaking agent provided in an embodiment of the present invention can be formulated brief, can quickly be made by preparation method
It is standby go out substantial amounts of paddy rice seed soaking agent；Paddy rice seed soaking agent prepared by the present invention can improve the emergence rate of rice to a certain extent again, and
Promote building up for rice seedling.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of paddy rice seed soaking agent, which is characterized in that the paddy rice seed soaking agent is mainly by S. ahygroscopicus Gongzhuling new variant
It is made with corn quarrel matrix.
2. paddy rice seed soaking agent according to claim 1, which is characterized in that the grain size of the corn quarrel matrix is 0.5-
0.7cm。
3. a kind of preparation method of paddy rice seed soaking agent, which is characterized in that comprise the following steps：
The quarrel matrix of the corn by immersion that grain size is 0.5-0.7cm is subjected to sterilization treatment；
S. ahygroscopicus Gongzhuling new variant of the inoculation Jing Guo activation culture is cultivated after cooling, obtains solid culture；
It dries and crushes the solid culture, obtain culture powder, the culture powder described in flooding collected 100-
The paddy rice seed soaking agent is made in the water extract by the water extract of 200 mesh sieves.
4. the preparation method of paddy rice seed soaking agent according to claim 3, which is characterized in that the corn quarrel matrix was impregnated
Time is 2h-4h.
5. the preparation method of paddy rice seed soaking agent according to claim 3, which is characterized in that the sterilization treatment is steamed for high pressure
Vapour sterilizes, and the temperature of the high pressure steam sterilization is 121 DEG C, time 20min.
6. the preparation method of paddy rice seed soaking agent according to claim 3, which is characterized in that the S. ahygroscopicus princess
The temperature conditionss of ridge new variant culture are 24.3 DEG C -30.6 DEG C, and the time is 5-7 days.
7. the preparation method of paddy rice seed soaking agent according to claim 6, which is characterized in that the extraction is using culture powder
The solid-liquid ratio of end and water is 1:2-4 is carried out.
8. such as application of the 1 or 2 any one of them paddy rice seed soaking agent of claim in Rice Cropping.
9. application of the paddy rice seed soaking agent according to claim 8 in Rice Cropping, which is characterized in that soak the rice
Kind of dilution agent is into seed soaking agent dilution, by rice paddy seed seed soaking agent dilution immersion 3-5 days, 20-30 DEG C of soaking temperature,
Accumulated temperature is 100 DEG C/day, pulls out to drain and moves to the germination of 28-33 DEG C of seedbed.
10. application of the paddy rice seed soaking agent according to claim 9 in Rice Cropping, which is characterized in that described diluted
Multiple is 100-1000 times.
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|---|---|---|---|---|
| CN113321554A (en) * | 2021-06-29 | 2021-08-31 | 吉林省农业科学院 | Disease control agent and application thereof, rice seedbed conditioner and preparation and use methods thereof |
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