CN113973827A - Rice seed coating agent for comprehensive planting and breeding of rice and shrimps and preparation method thereof - Google Patents
Rice seed coating agent for comprehensive planting and breeding of rice and shrimps and preparation method thereof Download PDFInfo
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- CN113973827A CN113973827A CN202111431252.6A CN202111431252A CN113973827A CN 113973827 A CN113973827 A CN 113973827A CN 202111431252 A CN202111431252 A CN 202111431252A CN 113973827 A CN113973827 A CN 113973827A
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- 241000209094 Oryza Species 0.000 title claims abstract description 96
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 96
- 235000009566 rice Nutrition 0.000 title claims abstract description 96
- 239000011248 coating agent Substances 0.000 title claims abstract description 76
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 238000009395 breeding Methods 0.000 title claims description 30
- 230000001488 breeding effect Effects 0.000 title claims description 30
- 241000143060 Americamysis bahia Species 0.000 title claims 2
- 238000002156 mixing Methods 0.000 claims abstract description 53
- 238000000855 fermentation Methods 0.000 claims abstract description 50
- 230000004151 fermentation Effects 0.000 claims abstract description 50
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 39
- 241000238557 Decapoda Species 0.000 claims abstract description 37
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 claims abstract description 23
- 240000003259 Brassica oleracea var. botrytis Species 0.000 claims abstract description 23
- 235000013580 sausages Nutrition 0.000 claims abstract description 23
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 15
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 14
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 14
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 11
- 239000004310 lactic acid Substances 0.000 claims abstract description 11
- 244000064622 Physalis edulis Species 0.000 claims abstract description 7
- 235000001982 Physalis edulis Nutrition 0.000 claims abstract description 7
- 239000003337 fertilizer Substances 0.000 claims description 68
- 239000000203 mixture Substances 0.000 claims description 63
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 claims description 28
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 claims description 28
- 235000010081 allicin Nutrition 0.000 claims description 28
- 239000012752 auxiliary agent Substances 0.000 claims description 21
- 239000011573 trace mineral Substances 0.000 claims description 21
- 235000013619 trace mineral Nutrition 0.000 claims description 21
- 240000004584 Tamarindus indica Species 0.000 claims description 14
- 235000004298 Tamarindus indica Nutrition 0.000 claims description 14
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 11
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 11
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 11
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 11
- 229910052796 boron Inorganic materials 0.000 claims description 11
- 229910052802 copper Inorganic materials 0.000 claims description 11
- 239000010949 copper Substances 0.000 claims description 11
- 239000011669 selenium Substances 0.000 claims description 11
- 229910052711 selenium Inorganic materials 0.000 claims description 11
- 238000007873 sieving Methods 0.000 claims description 11
- 239000011701 zinc Substances 0.000 claims description 11
- 229910052725 zinc Inorganic materials 0.000 claims description 11
- 238000000576 coating method Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 9
- 238000000227 grinding Methods 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 244000045232 Canavalia ensiformis Species 0.000 claims description 6
- 235000010617 Phaseolus lunatus Nutrition 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 3
- 239000002270 dispersing agent Substances 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 2
- 239000002671 adjuvant Substances 0.000 claims 1
- 241000238017 Astacoidea Species 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 8
- 230000035784 germination Effects 0.000 abstract description 8
- 241000607479 Yersinia pestis Species 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 239000002689 soil Substances 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 244000046052 Phaseolus vulgaris Species 0.000 abstract description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 239000003630 growth substance Substances 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 10
- 229920002401 polyacrylamide Polymers 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 229920001661 Chitosan Polymers 0.000 description 7
- 229920001732 Lignosulfonate Polymers 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 7
- 239000002253 acid Substances 0.000 description 7
- 229940051841 polyoxyethylene ether Drugs 0.000 description 7
- 229920000056 polyoxyethylene ether Polymers 0.000 description 7
- 238000011282 treatment Methods 0.000 description 3
- 241000193755 Bacillus cereus Species 0.000 description 2
- 241000194103 Bacillus pumilus Species 0.000 description 2
- 229920000858 Cyclodextrin Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000194105 Paenibacillus polymyxa Species 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 241000220317 Rosa Species 0.000 description 2
- 238000003501 co-culture Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- -1 polyoxyethylene Polymers 0.000 description 2
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 241000863030 Lysobacter enzymogenes Species 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- TUXJTJITXCHUEL-UHFFFAOYSA-N disperse red 11 Chemical compound C1=CC=C2C(=O)C3=C(N)C(OC)=CC(N)=C3C(=O)C2=C1 TUXJTJITXCHUEL-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 230000035929 gnawing Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 241000238565 lobster Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001610 polycaprolactone Polymers 0.000 description 1
- 239000004632 polycaprolactone Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- AJTPODXVRWXEMS-UHFFFAOYSA-M potassium;4-(1h-indol-2-yl)butanoate Chemical compound [K+].C1=CC=C2NC(CCCC(=O)[O-])=CC2=C1 AJTPODXVRWXEMS-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Inorganic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a rice seed coating agent for comprehensive planting of rice and shrimp and a preparation method thereof, fermentation liquor obtained by mixing and fermenting sausage fruit, cape gooseberry, cauliflower and sour bean leaves can emit unpleasant smell of crayfish and pests, prevents crayfish and pests from approaching seeds, has a growth promoting effect on rice, can improve the germination effect of rice seeds, can reduce the use of growth regulators, adopts lactic acid bacteria, bacillus coagulans and bacillus subtilis to ferment sausage fruit, cape gooseberry, cauliflower and sour bean leaves, can improve the micro-ecological environment of rice field soil, improves the root activity of seedlings, promotes the growth of the seedlings, and can effectively reduce the incidence of water bakanae disease and bacterial brown spot disease by matching with jinggangmycin and garlicin.
Description
Technical Field
The invention relates to the technical field of rice seed coating agents, in particular to a rice seed coating agent for comprehensive breeding of rice and shrimps and a preparation method thereof.
Background
The comprehensive breeding of the rice and the shrimps is to breed the lobsters in the rice field, promote the virtuous cycle of energy and substances in an ecological system of the rice field, remove weeds and pests in the rice field by utilizing the activities of the crayfishes and loosen soil, on the contrary, the weeds and the pests in the rice field can be used as baits of the crayfishes to provide nutrients for the growth and development of the crayfishes, and the excrement of the crayfishes can also be used as fertilizers of the rice to realize the double harvest of the rice and the shrimps. The comprehensive breeding of rice and shrimp is a high-efficiency three-dimensional ecological system for realizing the co-culture of rice and shrimp by comprehensively utilizing the ecological characteristics of rice and lobster.
The crayfish inhabits and eats in the field, can loosen soil, improve the ventilation condition of the soil and accelerate the decomposition of fertilizer, but the crayfish has complicated feeding property and is easy to damage the seeds and seedlings of rice. Therefore, when the rice is planted, the seeds need to be cultivated into seedlings in other environments to be transplanted into the rice field, and in the initial stage of planting and seedling transplanting, the crayfishes cannot be cultivated in the rice field, the cultivation period of the crayfishes is short, and the rice field resources cannot be fully utilized.
The seed coating agent is an agent coated on the seed body, and can form a protective layer with certain functions and coating strength outside the seed. The seed coating agent has obvious effect on improving the emergence rate of seeds and preventing and treating the diseases and pests of crops in the seedling stage, and has the effects of preventing nutrient deficiency, stimulating growth, increasing the yield of crops, improving quality and the like. Seed coating liquids, also in solid powder form, such as: patent CN106577772A "a new biological seed coating agent and its application" discloses a seed coating agent prepared by using a mixed fermentation broth of specific Paenibacillus polymyxa (Paenibacillus polymyxa), Bacillus cereus (Bacillus cereus) and Bacillus pumilus (Bacillus pumilus); CN109645028A patent "seed coating agent containing OH 11" discloses seed coating agent consisting of lysobacter enzymogenes OH11 and potassium indolebutyrate ".
At present, the probability of coating rice seeds in China is extremely low, the main reasons are that the proper seed coating agent is few, the coating and using technology is not proper, and the like, and the existing seed coating agent contains insecticide or bactericide and cannot be used as the seed coating agent of the rice seeds in the rice shrimp co-culture. Therefore, there is a need to develop a rice seed coating agent and a coating method thereof, which can be used for the comprehensive breeding of rice and shrimp.
Disclosure of Invention
In order to solve the problems, the invention provides a rice seed coating agent for comprehensive planting of rice and shrimps and a preparation method thereof.
The invention relates to a rice seed coating agent for comprehensive planting and breeding of rice and shrimps, which comprises the following components: according to the weight parts, 0.02-0.03 part of jinggangmycin, 0.01-0.02 part of allicin, 28-36 parts of fermentation liquor, 15.5-19 parts of trace element fertilizer and 5-10 parts of auxiliary agent;
the fermentation liquor is obtained by fermenting sausage fruit, lantern fruit, cauliflower and lima bean leaves.
Further, the mass ratio of the sausage fruit, the lantern fruit, the cauliflower and the tamarind leaf is (0.4-0.5): 1 (0.8-1.2): (0.2-0.5).
Further, the fermentation is carried out by using lactic acid bacteria, bacillus coagulans and bacillus subtilis.
Further, the trace element fertilizer comprises a zinc fertilizer, a selenium fertilizer, a copper fertilizer and a boron fertilizer.
Furthermore, the mass ratio of the zinc fertilizer, the selenium fertilizer, the copper fertilizer and the boron fertilizer is 1 (0.2-0.3) to 0.8-1.1 to 0.6-0.9.
Further, the auxiliary agent comprises: film forming agent, dispersant, slow release agent and warning color.
Further, the film forming agent is at least one of polyacrylamide, chitosan, polyvinyl alcohol and polycaprolactone.
Further, the dispersing agent is at least one of lignosulfonate, alkylphenol polyoxyethylene and fatty alcohol polyoxyethylene.
Further, the slow release agent is at least one of cyclodextrin, diatomite and bentonite.
Further, the warning color is at least one of acid red, disperse red and rose essence.
Furthermore, the preparation method of the rice seed coating agent for the comprehensive breeding of rice and shrimps comprises the following steps:
a. pretreatment of raw materials: respectively grinding and sieving jinggangmycin, allicin, trace element fertilizer and auxiliary agent; then respectively cleaning sausage fruit, cape gooseberry, cauliflower and lima bean leaves, airing, mixing and crushing to obtain a mixture 1;
b. preparing fermentation liquor: adding mixed bacteria of lactobacillus, bacillus coagulans and bacillus subtilis into the mixture 1, fermenting for 5-7 d, and filtering to obtain fermentation liquor;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
Further, in the step a, the sieving is performed by sieving with a 100-200-mesh sieve.
Further, in the step b, the using amount of the mixed bacteria is 0.7-0.8% of the weight of the mixture 1; the number of the mixed bacteria per 1g is 1 × 108～9CFU/g。
Further, in the step b, the mixed bacteria are obtained by mixing lactic acid bacteria, bacillus coagulans and bacillus subtilis according to the quantity ratio of 1 (0.3-0.6) to (1.2-1.8).
Further, in the step b, the fermentation temperature is 25-30 ℃, and the humidity is 60-80%.
Furthermore, the using amount of the seed coating agent is 0.3-0.45 kg of the seed coating agent added into every 1kg of seeds.
Compared with the prior art, the invention has the beneficial effects that:
the rice seed coating agent for comprehensively planting rice and shrimps, disclosed by the invention, is a fermentation liquid obtained by mixing and fermenting sausage fruit, cape gooseberry, cauliflower and sour bean leaves, can give out unpleasant odor of crayfish and pests, prevents the crayfish and the pests from approaching seeds, has a growth promoting effect on rice, can improve the germination effect of rice seeds, can reduce the use of a growth regulator, and can effectively reduce the morbidity of water bakanae disease and bacterial brown spot by matching with jinggangmycin and allicin.
According to the invention, the lactobacillus, the bacillus coagulans and the bacillus subtilis are used for fermenting the sausage fruit, the lantern fruit, the cauliflower and the tamarind leaf, and the fermentation liquor obtained after fermentation can improve the micro-ecological environment of the rice field soil, improve the root activity of seedlings and promote the growth of the seedlings.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the present invention can be obtained commercially without specific description.
Example 1
A rice seed coating agent for comprehensive breeding of rice and shrimp comprises the following components: 0.02g of jinggangmycin, 0.01g of allicin, 28g of fermentation liquor, 6.55g of zinc fertilizer, 1.31g of selenium fertilizer, 7.21g of copper fertilizer, 3.93g of boron fertilizer, 5.2g of polyacrylamide, 3.4g of fatty alcohol-polyoxyethylene ether, 1.3g of cyclodextrin and 0.1g of rose essence;
a preparation method of a rice seed coating agent for comprehensive breeding of rice and shrimps comprises the following steps:
a. pretreatment of raw materials: respectively grinding the jinggangmycin, the allicin, the trace element fertilizer and the auxiliary agent, and then sieving the ground materials by a 100-mesh sieve; then respectively cleaning sausage fruit, lantern fruit, cauliflower and tamarind leaf, airing, and then mixing according to the weight ratio of 0.4: 1:1.2: 0.2, mixing the sausage fruit, the lantern fruit, the cauliflower and the tamarind leaf, and crushing to obtain a mixture 1;
b. preparing fermentation liquor: adding mixed bacteria accounting for 0.7 percent of the weight of the mixture 1 into the mixture 1, fermenting for 5-7 days at the temperature of 25-30 ℃ and the humidity of 60-80 percent, and filtering to obtain fermentation liquor; the mixed bacteria are obtained by mixing lactic acid bacteria, bacillus coagulans and bacillus subtilis according to the quantity ratio of 1:0.5: 1.8;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
Example 2
A rice seed coating agent for comprehensive breeding of rice and shrimp comprises the following components: 0.03g of jinggangmycin, 0.02g of allicin, 32g of fermentation liquor, 5.97g of zinc fertilizer, 1.79g of selenium fertilizer, 5.97g of copper fertilizer, 4.77g of boron fertilizer, 2.6g of polyacrylamide, 1.7g of chitosan, 1.6g of lignosulfonate, 1g of fatty alcohol-polyoxyethylene ether, 1.1g of diatomite and 0.2g of acid red;
a preparation method of a rice seed coating agent for comprehensive breeding of rice and shrimps comprises the following steps:
a. pretreatment of raw materials: respectively grinding the jinggangmycin, the allicin, the trace element fertilizer and the auxiliary agent, and then sieving by a 200-mesh sieve; then respectively cleaning sausage fruit, lantern fruit, cauliflower and tamarind leaf, airing, and then mixing according to the weight ratio of 0.5: 1:1.1: 0.3, mixing the sausage fruit, the lantern fruit, the cauliflower and the tamarind leaf, and crushing to obtain a mixture 1;
b. preparing fermentation liquor: adding mixed bacteria accounting for 0.8 percent of the weight of the mixture 1 into the mixture 1, fermenting for 5-7 days at the temperature of 25-30 ℃ and the humidity of 60-80 percent, and filtering to obtain fermentation liquor; the mixed bacteria are obtained by mixing lactic acid bacteria, bacillus coagulans and bacillus subtilis according to the quantity ratio of 1:0.3: 1.5;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
Example 3
A rice seed coating agent for comprehensive breeding of rice and shrimp comprises the following components: 0.03g of jinggangmycin, 0.02g of allicin, 32g of fermentation liquor, 5.97g of zinc fertilizer, 1.79g of selenium fertilizer, 5.97g of copper fertilizer, 4.77g of boron fertilizer, 2.6g of polyacrylamide, 1.7g of chitosan, 1.6g of lignosulfonate, 1g of fatty alcohol-polyoxyethylene ether, 1.1g of diatomite and 0.2g of acid red;
a preparation method of a rice seed coating agent for comprehensive breeding of rice and shrimps comprises the following steps:
a. pretreatment of raw materials: respectively grinding the jinggangmycin, the allicin, the trace element fertilizer and the auxiliary agent, and then sieving by a 200-mesh sieve; then respectively cleaning sausage fruit, lantern fruit, cauliflower and tamarind leaf, airing, and then mixing according to the weight ratio of 0.5: 1:1.1: 0.3, mixing the sausage fruit, the lantern fruit, the cauliflower and the tamarind leaf, and crushing to obtain a mixture 1;
b. preparing fermentation liquor: adding bacillus subtilis accounting for 0.8% of the weight of the mixture 1 into the mixture 1, fermenting for 5-7 days at the temperature of 25-30 ℃ and under the humidity of 60-80%, and filtering to obtain fermentation liquor;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
Comparative example 1
A rice seed coating agent for comprehensive breeding of rice and shrimp comprises the following components: 0.03g of jinggangmycin, 0.02g of allicin, 32g of fermentation liquor, 5.97g of zinc fertilizer, 1.79g of selenium fertilizer, 5.97g of copper fertilizer, 4.77g of boron fertilizer, 2.6g of polyacrylamide, 1.7g of chitosan, 1.6g of lignosulfonate, 1g of fatty alcohol-polyoxyethylene ether, 1.1g of diatomite and 0.2g of acid red;
a preparation method of a rice seed coating agent for comprehensive breeding of rice and shrimps comprises the following steps:
a. pretreatment of raw materials: respectively grinding the jinggangmycin, the allicin, the trace element fertilizer and the auxiliary agent, and then sieving by a 200-mesh sieve; then, washing the cape gooseberry, the cauliflower and the tamarind tree leaves respectively, airing, and mixing according to a ratio of 1: 1.1: mixing and crushing cape gooseberry, cauliflower and lima bean leaves according to the mass ratio of 0.3 to obtain a mixture 1;
b. preparing fermentation liquor: adding mixed bacteria accounting for 0.8 percent of the weight of the mixture 1 into the mixture 1, fermenting for 5-7 days at the temperature of 25-30 ℃ and the humidity of 60-80 percent, and filtering to obtain fermentation liquor; the mixed bacteria are obtained by mixing lactic acid bacteria, bacillus coagulans and bacillus subtilis according to the quantity ratio of 1:0.3: 1.5;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
Comparative example 2
A rice seed coating agent for comprehensive breeding of rice and shrimp comprises the following components: 0.03g of jinggangmycin, 0.02g of allicin, 32g of fermentation liquor, 5.97g of zinc fertilizer, 1.79g of selenium fertilizer, 5.97g of copper fertilizer, 4.77g of boron fertilizer, 2.6g of polyacrylamide, 1.7g of chitosan, 1.6g of lignosulfonate, 1g of fatty alcohol-polyoxyethylene ether, 1.1g of diatomite and 0.2g of acid red;
a preparation method of a rice seed coating agent for comprehensive breeding of rice and shrimps comprises the following steps:
a. pretreatment of raw materials: respectively grinding the jinggangmycin, the allicin, the trace element fertilizer and the auxiliary agent, and then sieving by a 200-mesh sieve; then respectively cleaning sausage fruit, cauliflower and lima bean leaves, airing, and mixing according to the weight ratio of 0.5: 1.1: 0.3, mixing the sausage fruit, the cauliflower and the tamarind leaf, and crushing to obtain a mixture 1;
b. preparing fermentation liquor: adding mixed bacteria accounting for 0.8 percent of the weight of the mixture 1 into the mixture 1, fermenting for 5-7 days at the temperature of 25-30 ℃ and the humidity of 60-80 percent, and filtering to obtain fermentation liquor; the mixed bacteria are obtained by mixing lactic acid bacteria, bacillus coagulans and bacillus subtilis according to the quantity ratio of 1:0.3: 1.5;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
Comparative example 3
A rice seed coating agent for comprehensive breeding of rice and shrimp comprises the following components: 0.03g of jinggangmycin, 0.02g of allicin, 32g of fermentation liquor, 5.97g of zinc fertilizer, 1.79g of selenium fertilizer, 5.97g of copper fertilizer, 4.77g of boron fertilizer, 2.6g of polyacrylamide, 1.7g of chitosan, 1.6g of lignosulfonate, 1g of fatty alcohol-polyoxyethylene ether, 1.1g of diatomite and 0.2g of acid red;
a preparation method of a rice seed coating agent for comprehensive breeding of rice and shrimps comprises the following steps:
a. pretreatment of raw materials: respectively grinding the jinggangmycin, the allicin, the trace element fertilizer and the auxiliary agent, and then sieving by a 200-mesh sieve; then respectively cleaning sausage fruit, lantern fruit and tamarind leaf, airing, and then mixing according to the weight ratio of 0.5: 1:0.3, mixing the sausage fruit, the lantern fruit and the tamarind tree leaves, and crushing to obtain a mixture 1;
b. preparing fermentation liquor: adding mixed bacteria accounting for 0.8 percent of the weight of the mixture 1 into the mixture 1, fermenting for 5-7 days at the temperature of 25-30 ℃ and the humidity of 60-80 percent, and filtering to obtain fermentation liquor; the mixed bacteria are obtained by mixing lactic acid bacteria, bacillus coagulans and bacillus subtilis according to the quantity ratio of 1:0.3: 1.5;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
Comparative example 4
A rice seed coating agent for comprehensive breeding of rice and shrimp comprises the following components: 0.03g of jinggangmycin, 0.02g of allicin, 32g of fermentation liquor, 5.97g of zinc fertilizer, 1.79g of selenium fertilizer, 5.97g of copper fertilizer, 4.77g of boron fertilizer, 2.6g of polyacrylamide, 1.7g of chitosan, 1.6g of lignosulfonate, 1g of fatty alcohol-polyoxyethylene ether, 1.1g of diatomite and 0.2g of acid red;
a preparation method of a rice seed coating agent for comprehensive breeding of rice and shrimps comprises the following steps:
a. pretreatment of raw materials: respectively grinding the jinggangmycin, the allicin, the trace element fertilizer and the auxiliary agent, and then sieving by a 200-mesh sieve; then respectively cleaning sausage fruit, lantern fruit and cauliflower, airing, and mixing according to the weight ratio of 0.5: mixing sausage fruit, lantern fruit and cauliflower in a mass ratio of 1:1.1, and crushing to obtain a mixture 1;
b. preparing fermentation liquor: adding mixed bacteria accounting for 0.8 percent of the weight of the mixture 1 into the mixture 1, fermenting for 5-7 days at the temperature of 25-30 ℃ and the humidity of 60-80 percent, and filtering to obtain fermentation liquor; the mixed bacteria are obtained by mixing lactic acid bacteria, bacillus coagulans and bacillus subtilis according to the quantity ratio of 1:0.3: 1.5;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
Test examples
1.1 design of the experiment
The test varieties are: boyou 225;
test time: 7 months in 2018 to 11 months in 2018;
test site: a Jones region of Haikou City, Hainan province;
grouping tests: the test is totally set with 8 treatments, wherein the treatments 1 to 7 are respectively to coat the rice seeds by using the seed coating agents of the examples 1 to 3 and the comparative examples 1 to 4, the dosage of the seed coating agent is 0.3kg of the seed coating agent per 1kg of the seeds, the treatment 8 is a blank control, no seed coating agent is used, 500 seeds are sown in each group, and the test area is 0.2 mu;
the test method comprises the following steps: uniformly mixing seeds and a seed coating agent, airing, sowing in a seedling raising tray, placing the seedling raising tray in a constant-temperature illumination condition for culture, calculating the germination vigor after the seeds germinate in a standard manner, and investigating the height and the root length of the seedlings 10 days after sowing, wherein the results are shown in table 1; then transferring the crayfish to a rice field for seedling raising, counting the eating rate and the seedling rate of the crayfish after 30d, and investigating the incidence rate of bakanae disease and bacterial brown spot by adopting a 5-point sampling method when the seedlings are in a three-leaf period, wherein the results are shown in a table 2, and the crayfish is normally raised in the rice field in the period.
Germination vigor (%) — (number of germinated seeds in 3 days ÷ number of test seeds) × 100%;
TABLE 1
| Germination vigor (%) | Miao height (cm) | Root length (cm) | |
| Example 1 | 87.6 | 10.7 | 7.1 |
| Example 2 | 90.8 | 11.5 | 7.3 |
| Example 3 | 83.2 | 10.6 | 6.9 |
| Comparative example 1 | 76.2 | 9.2 | 6.5 |
| Comparative example 2 | 72.6 | 9.3 | 6.1 |
| Comparative example 3 | 61.2 | 8.7 | 5.6 |
| Comparative example 4 | 68.0 | 8.9 | 5.9 |
| Blank control | 78.2 | 9.4 | 6.7 |
As can be seen from Table 1, the seed coating agent provided by the invention can significantly improve the germination vigor of rice seeds, can also promote the growth of seedlings and promote the development of root systems, and compared with a blank control group, the germination vigor is improved by 6.39-16.11%, the seedling height is 12.77-22.34%, and the root length is 2.99-8.94%, wherein the seed coating agent in example 3 has the worst effect; compared with comparative examples 1-4, the germination vigor of the rice seeds is obviously improved, the seedling height and the root length are obviously increased, and the growth promoting effect of the fermentation liquor obtained by performing mixed fermentation on the sausage fruit, the lantern fruit, the cauliflower and the tamarind leaf is good.
TABLE 2
As can be seen from Table 2, the seed coating agent of the invention can effectively avoid the gnawing of crayfish, reduce the incidence of bakanae disease and bacterial brown spot and improve the seedling rate.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A rice seed coating agent for comprehensive planting and breeding of rice and shrimps is characterized by comprising the following components: according to the weight parts, 0.02-0.03 part of jinggangmycin, 0.01-0.02 part of allicin, 28-36 parts of fermentation liquor, 15.5-19 parts of trace element fertilizer and 5-10 parts of auxiliary agent;
the fermentation liquor is obtained by fermenting sausage fruit, lantern fruit, cauliflower and lima bean leaves.
2. The rice seed coating for the comprehensive planting and breeding of rice and shrimp as claimed in claim 1, wherein the mass ratio of the sausage fruit, the lantern fruit, the cauliflower and the tamarind leaf is (0.4-0.5): 1 (0.8-1.2): (0.2-0.5).
3. The rice seed coating for integrated breeding of rice and shrimp according to claim 1, wherein said fermentation is a fermentation using lactic acid bacteria, bacillus coagulans and bacillus subtilis.
4. The rice seed coating for the comprehensive planting and breeding of rice and shrimp as claimed in claim 1, wherein said microelement fertilizer comprises zinc fertilizer, selenium fertilizer, copper fertilizer and boron fertilizer; the mass ratio of the zinc fertilizer, the selenium fertilizer, the copper fertilizer and the boron fertilizer is 1 (0.2-0.3) to 0.8-1.1) to 0.6-0.9.
5. The rice seed coating agent for the comprehensive breeding of rice and shrimp as claimed in claim 1, wherein said adjuvant comprises: film forming agent, dispersant, slow release agent and warning color.
6. The preparation method of the rice seed coating agent for the comprehensive breeding of rice and shrimp as claimed in claim 3, comprising the steps of:
a. pretreatment of raw materials: respectively grinding and sieving jinggangmycin, allicin, trace element fertilizer and auxiliary agent; then respectively cleaning sausage fruit, cape gooseberry, cauliflower and lima bean leaves, airing, mixing and crushing to obtain a mixture 1;
b. preparing fermentation liquor: adding mixed bacteria of lactobacillus, bacillus coagulans and bacillus subtilis into the mixture 1, fermenting for 5-7 d, and filtering to obtain fermentation liquor;
c. preparing a seed coating agent: uniformly mixing the fermentation liquor and the auxiliary agent to obtain a mixture 2, uniformly mixing the trace element fertilizer, the jinggangmycin and the allicin to obtain a mixture 3, and uniformly mixing the mixture 2 and the mixture 3 to obtain the seed coating agent.
7. The preparation method of the rice seed coating for the comprehensive planting of rice and shrimp as claimed in claim 6, wherein in step b, the amount of the mixed bacteria is 0.7-0.8% of 1 weight of the mixture; the number of the mixed bacteria per 1g is 1 × 108～9。
8. The method for preparing a rice seed coating agent for the comprehensive planting of rice and shrimp as claimed in claim 7, wherein in the step b, the mixed bacteria are lactic acid bacteria, bacillus coagulans and bacillus subtilis which are mixed according to the quantity ratio of 1 (0.3-0.6) to (1.2-1.8).
9. The preparation method of the rice seed coating for the comprehensive planting and breeding of rice and shrimp as claimed in claim 6, wherein in the step b, the fermentation temperature is 25-30 ℃ and the humidity is 60-80%.
10. The rice seed coating agent for the comprehensive planting and breeding of rice and shrimp as claimed in any one of claims 1-9, wherein the amount of the seed coating agent added is 0.3-0.45 kg per 1kg of seeds.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461386A (en) * | 2013-08-23 | 2013-12-25 | 河南省农业科学院植物保护研究所 | Composite sesame seed coating agent |
| CN107926998A (en) * | 2017-11-14 | 2018-04-20 | 张静 | A kind of preparation method of seed coat agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103461386A (en) * | 2013-08-23 | 2013-12-25 | 河南省农业科学院植物保护研究所 | Composite sesame seed coating agent |
| CN107926998A (en) * | 2017-11-14 | 2018-04-20 | 张静 | A kind of preparation method of seed coat agent |
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