CN113711907A - Method for producing seeds by muskmelon hybridization - Google Patents

Method for producing seeds by muskmelon hybridization Download PDF

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CN113711907A
CN113711907A CN202110876779.3A CN202110876779A CN113711907A CN 113711907 A CN113711907 A CN 113711907A CN 202110876779 A CN202110876779 A CN 202110876779A CN 113711907 A CN113711907 A CN 113711907A
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male
parent
female
female parent
stigma
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朱忠厚
朱学杰
朱江锋
朱艳强
左宜赞
朱学民
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Luoyang City Agricultural Development Technology Co ltd
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Luoyang City Agricultural Development Technology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12Processes for modifying agronomic input traits, e.g. crop yield

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  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention belongs to the field of biotechnology, and particularly relates to a muskmelon hybrid seed production method, which adopts sandy soil with deep coating, good permeability and difficult water accumulation as muskmelon hybrid seed production land, transplanting the germinated female parent and the male parent in a field planting mode respectively to buckle a small arched shed, removing the small arched shed after the female parent and the male parent are planted for one month, pruning two vines of the female parent and pruning three vines of the male parent; removing all male flowers on the female parent plant before pollination, sleeving an isolated melon cap to ensure that the female parent has no male flowers, reserving all male flowers on the male parent, and picking all male flowers and young fruits; carrying out hybrid pollination after 4-5 female flowers grow on the lateral vines; coating the stigma of the male parent male flower on the stigma of the female parent female flower, and then sheathing an isolating melon cap; the flour is added into the pollen, so that the pollen waste is avoided; the reasonable planting proportion is set, so that the pollination efficiency of the male parent seeds is ensured under the condition of using the minimum number of the male parent seeds, the muskmelon fruiting rate is also obviously improved compared with other methods, and the vitality of the stigma is maintained by adopting a topping mode.

Description

Method for producing seeds by muskmelon hybridization
Technical Field
The invention belongs to the field of biotechnology, and particularly relates to a method for producing seeds by muskmelon hybridization.
Background
The muskmelon is a fruit melon which is widely planted in various parts of China, has different tastes, shapes and colors, and is mature and marketed in summer. The whole melon plant also has certain medicinal value and has the effects of emetic, dehumidification, jaundice elimination and the like.
The muskmelon is generally bred by adopting seeds during planting, the variety is more, and plastic films and glass which are high in transparency and free of water drops are used as much as possible during planting; in order to improve the planting benefit of farmers, the fruit bearing rate of the melons is improved by carrying out hybridization research on the melons which are high-efficiency economic crops with river characteristics, the new variety obtained by hybridization can overcome the climate defects of surplus maturity in part of regions and insufficient maturity of two crops, the yield and the quality are improved, and the high benefit of two crops in one year is realized.
The existing hybrid seed production of melons still has the problem of low seed production rate, the main reason is that the yield of hybrid melons is low, and therefore, a new method needs to be developed to improve the hybrid seed production rate of melons.
Disclosure of Invention
The invention aims to provide a method for producing hybrid seeds of melons, which solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for producing seeds by muskmelon hybridization comprises the following steps:
selecting a sandy soil with a deep coating, good permeability and low water accumulation tendency as a muskmelon hybridization seed production field, selecting a male parent and a female parent with the purity of 95%, separately soaking the male parent and the female parent in potassium permanganate solution for 0.5 hour, then soaking in warm water at 50 ℃ for 10 minutes, accelerating germination at 27 ℃ for 18 hours, and then sowing and raising seedlings;
step two, transplanting management, namely transplanting the germinated female parent and the germinated male parent in small arched sheds respectively, wherein the transplanting plant spacing is 0.5 m; watering and topdressing when the seedlings grow to 6-8 true leaves, opening the small arched shed in high-temperature weather, and removing the small arched shed after field planting for one month, wherein the female parent carries out two-vine pruning and the male parent carries out three-vine pruning;
step three, cross pollination, wherein all male flowers on the female parent plant are removed and the melon cap is isolated in a sleeving manner before pollination, so that the female parent is ensured to have no male flowers, all male flowers on the male parent are reserved, and all male flowers and young fruits are picked off; carrying out hybrid pollination after 4-5 female flowers grow on the lateral vines;
coating the stigma of the male parent male flower on the stigma of the female parent female flower, and then sheathing an isolating melon cap;
step four, seed picking, namely picking the fully mature fruits and placing for half a month when the fruits begin to be colored and mature, cutting the outer edges of the peels and picking the seeds to avoid scratching the seeds; cleaning, rinsing and removing shriveled seeds after seed picking, and drying; and after the water content of the seeds is reduced to below 7%, further selecting, packaging and warehousing.
As a further scheme of the invention: in the first step, the male parent and the female parent are planted according to a ratio of 1:5, imidacloprid missible oil is sprayed to prevent and control aphids after planting, the planting of the male parent is half a month earlier than that of the female parent, film covering treatment is carried out after seeding and seedling raising, and after accelerating germination for 18 hours, the seeds are sowed and seedling raised after exposing the cuspids.
As a still further scheme of the invention: in the second step, after the young bud cotyledon is completely unfolded after field planting and transplanting, timely releasing air, and spraying 45% dimethoate missible oil to control pests; the top dressing mainly comprises compound fertilizer, phosphorus fertilizer, potassium fertilizer and farmyard manure.
As a still further scheme of the invention: in the third step, when the female parent plant is in the full-bloom stage, the stigma from 1 day before blooming to 1 day after blooming is selected, and the stigma which is not cracked is used as the stigma of the female parent female flower for pollination; the fruit bearing on the female parent plant is cut off, the flower and the flower bud are left, and the flowering corolla is torn off to expose the stigma.
As a still further scheme of the invention: in the third step, the male parent and the male parent are anthers collected in the initial flowering period of the male parent, pollen on the anther wall is removed through a 80-mesh sieve after the anthers are dried and cracked, the sieved pollen and the white surface are mixed in a ratio of 1:3, female parent selected by pollination is female flower corolla reddish until the corolla is cracked, and the stigma is 2 rows or 3 rows.
As a still further scheme of the invention: in the third step, the male flowers on the female parent plants are hit out and torn off by pinching the joints of the petals and the sepals with fingers, and the male flowers are completely removed; when pollinating, the eraser at the tail of the pencil is used for dipping pollen of the male parent and coating the pollen on the stigma of the female parent; a multi-male flower can pollinate 3-5 female flowers.
As a still further scheme of the invention: in the third step, topping treatment is carried out after pollination, all growing points are knocked off, water and fertilizer supply is increased, watering is carried out once a week, top dressing is carried out once every half month, the types of top dressing mainly comprise urea and monopotassium phosphate, and the proportion of the urea to the monopotassium phosphate is 2: 1; and the pesticide is sprayed every 10 days, and the pesticide comprises 1000 times of liquid of 1.5 percent of carbendazim and 500 times of liquid of 40 percent of virobuzin wettable powder for preventing and treating virus diseases.
Compared with the prior art, the invention has the beneficial effects that: the method of the invention has low cost for crossbreeding melon, and the waste of pollen is avoided by adding flour into pollen because the amount of pollen required for pollination is small; the reasonable planting proportion is set, so that the pollination efficiency of the male parent seeds is ensured under the condition of using the minimum number of the male parent seeds, the muskmelon fruiting rate is also obviously improved compared with other methods, and the vitality of the stigma is maintained by adopting a topping mode.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
In the embodiment of the invention, the method for producing seeds by crossbreeding melons comprises the following steps:
selecting a sandy soil with a deep coating, good permeability and low water accumulation tendency as a muskmelon hybridization seed production field, selecting a male parent and a female parent with the purity of 95%, separately soaking the male parent and the female parent in potassium permanganate solution for 0.5 hour, then soaking in warm water at 50 ℃ for 10 minutes, accelerating germination at 27 ℃ for 18 hours, and then sowing and raising seedlings;
wherein, the germination rate can be ensured by adopting the male parent and the female parent with higher purity, and the centralized pollination is convenient;
step two, transplanting management, namely transplanting the germinated female parent and the germinated male parent in small arched sheds respectively, wherein the transplanting plant spacing is 0.5 m; watering and topdressing when the seedlings grow to 6-8 true leaves, opening the small arched shed in high-temperature weather, and removing the small arched shed after field planting for one month, wherein the female parent carries out two-vine pruning and the male parent carries out three-vine pruning;
step three, cross pollination, wherein all male flowers on the female parent plant are removed and the melon cap is isolated in a sleeving manner before pollination, so that the female parent is ensured to have no male flowers, all male flowers on the male parent are reserved, and all male flowers and young fruits are picked off; carrying out hybrid pollination after 4-5 female flowers grow on the lateral vines;
coating the stigma of the male parent male flower on the stigma of the female parent female flower, and then sheathing an isolating melon cap;
step four, seed picking, namely picking the fully mature fruits and placing for half a month when the fruits begin to be colored and mature, cutting the outer edges of the peels and picking the seeds to avoid scratching the seeds; cleaning, rinsing and removing shriveled seeds after seed picking, and drying; and after the water content of the seeds is reduced to below 7%, further selecting, packaging and warehousing.
Example 2
In the embodiment of the invention, the method for producing seeds by crossbreeding melons comprises the following steps:
selecting a sandy soil with a deep coating, good permeability and low water accumulation tendency as a muskmelon hybridization seed production field, selecting a male parent and a female parent with the purity of 95%, separately soaking the male parent and the female parent in potassium permanganate solution for 0.5 hour, then soaking in warm water at 50 ℃ for 10 minutes, accelerating germination at 27 ℃ for 18 hours, and then sowing and raising seedlings;
wherein, the germination rate can be ensured by adopting the male parent and the female parent with higher purity, and the centralized pollination is convenient;
in the first step, the male parent and the female parent are planted according to a ratio of 1:5, imidacloprid missible oil is sprayed to prevent and control aphids after planting, the planting of the male parent is half a month earlier than that of the female parent, film covering treatment is carried out after seeding and seedling raising, and after accelerating germination for 18 hours, the seeds are sowed and seedling raised after exposing the cuspids;
the purpose of planting the male parents in advance is to ensure that the flowering phases of the male parents and the female parents can meet, and the purpose of higher number of the male parents is to ensure that the number of male flowers is sufficient and the pollen is more in the later period;
step two, transplanting management, namely transplanting the germinated female parent and the germinated male parent in small arched sheds respectively, wherein the transplanting plant spacing is 0.5 m; watering and topdressing when the seedlings grow to 6-8 true leaves, opening the small arched shed in high-temperature weather, and removing the small arched shed after field planting for one month, wherein the female parent carries out two-vine pruning and the male parent carries out three-vine pruning;
step three, cross pollination, wherein all male flowers on the female parent plant are removed and the melon cap is isolated in a sleeving manner before pollination, so that the female parent is ensured to have no male flowers, all male flowers on the male parent are reserved, and all male flowers and young fruits are picked off; carrying out hybrid pollination after 4-5 female flowers grow on the lateral vines;
coating the stigma of the male parent male flower on the stigma of the female parent female flower, and then sheathing an isolating melon cap;
step four, seed picking, namely picking the fully mature fruits and placing for half a month when the fruits begin to be colored and mature, cutting the outer edges of the peels and picking the seeds to avoid scratching the seeds; cleaning, rinsing and removing shriveled seeds after seed picking, and drying; and after the water content of the seeds is reduced to below 7%, further selecting, packaging and warehousing.
Example 3
In the embodiment of the invention, the method for producing seeds by crossbreeding melons comprises the following steps:
selecting a sandy soil with a deep coating, good permeability and low water accumulation tendency as a muskmelon hybridization seed production field, selecting a male parent and a female parent with the purity of 95%, separately soaking the male parent and the female parent in potassium permanganate solution for 0.5 hour, then soaking in warm water at 50 ℃ for 10 minutes, accelerating germination at 27 ℃ for 18 hours, and then sowing and raising seedlings;
wherein, the germination rate can be ensured by adopting the male parent and the female parent with higher purity, and the centralized pollination is convenient;
step two, transplanting management, namely transplanting the germinated female parent and the germinated male parent in small arched sheds respectively, wherein the transplanting plant spacing is 0.5 m; watering and topdressing when the seedlings grow to 6-8 true leaves, opening the small arched shed in high-temperature weather, and removing the small arched shed after field planting for one month, wherein the female parent carries out two-vine pruning and the male parent carries out three-vine pruning;
in the second step, after the young bud cotyledon is completely unfolded after field planting and transplanting, timely releasing air, and spraying 45% dimethoate missible oil to control pests; the top dressing mainly comprises compound fertilizer, phosphorus fertilizer, potassium fertilizer and farmyard manure; the small arched shed is opened in high temperature to prevent seedling burning;
step three, cross pollination, wherein all male flowers on the female parent plant are removed and the melon cap is isolated in a sleeving manner before pollination, so that the female parent is ensured to have no male flowers, all male flowers on the male parent are reserved, and all male flowers and young fruits are picked off; carrying out hybrid pollination after 4-5 female flowers grow on the lateral vines;
coating the stigma of the male parent male flower on the stigma of the female parent female flower, and then sheathing an isolating melon cap;
step four, seed picking, namely picking the fully mature fruits and placing for half a month when the fruits begin to be colored and mature, cutting the outer edges of the peels and picking the seeds to avoid scratching the seeds; cleaning, rinsing and removing shriveled seeds after seed picking, and drying; and after the water content of the seeds is reduced to below 7%, further selecting, packaging and warehousing.
Example 4
In the embodiment of the invention, the method for producing seeds by crossbreeding melons comprises the following steps:
selecting a sandy soil with a deep coating, good permeability and low water accumulation tendency as a muskmelon hybridization seed production field, selecting a male parent and a female parent with the purity of 95%, separately soaking the male parent and the female parent in potassium permanganate solution for 0.5 hour, then soaking in warm water at 50 ℃ for 10 minutes, accelerating germination at 27 ℃ for 18 hours, and then sowing and raising seedlings;
wherein, the germination rate can be ensured by adopting the male parent and the female parent with higher purity, and the centralized pollination is convenient;
in the first step, the male parent and the female parent are planted according to a ratio of 1:5, imidacloprid missible oil is sprayed to prevent and control aphids after planting, the planting of the male parent is half a month earlier than that of the female parent, film covering treatment is carried out after seeding and seedling raising, and after accelerating germination for 18 hours, the seeds are sowed and seedling raised after exposing the cuspids;
the purpose of planting the male parents in advance is to ensure that the flowering phases of the male parents and the female parents can meet, and the purpose of higher number of the male parents is to ensure that the number of male flowers is sufficient and the pollen is more in the later period;
step two, transplanting management, namely transplanting the germinated female parent and the germinated male parent in small arched sheds respectively, wherein the transplanting plant spacing is 0.5 m; watering and topdressing when the seedlings grow to 6-8 true leaves, opening the small arched shed in high-temperature weather, and removing the small arched shed after field planting for one month, wherein the female parent carries out two-vine pruning and the male parent carries out three-vine pruning;
in the second step, after the young bud cotyledon is completely unfolded after field planting and transplanting, timely releasing air, and spraying 45% dimethoate missible oil to control pests; the top dressing mainly comprises compound fertilizer, phosphorus fertilizer, potassium fertilizer and farmyard manure; the small arched shed is opened in high temperature to prevent seedling burning;
step three, cross pollination, wherein all male flowers on the female parent plant are removed and the melon cap is isolated in a sleeving manner before pollination, so that the female parent is ensured to have no male flowers, all male flowers on the male parent are reserved, and all male flowers and young fruits are picked off; carrying out hybrid pollination after 4-5 female flowers grow on the lateral vines;
coating the stigma of the male parent male flower on the stigma of the female parent female flower, and then sheathing an isolating melon cap;
wherein, when the female parent plant is in the full-bloom stage, the stigma from 1 day before blooming to 1 day after blooming is selected, and the stigma which is not cracked is taken as the stigma of the female parent female flower for pollination; cutting off the fruits on the female parent plant, leaving flowers and buds, and tearing off the bloomed corolla to expose the stigma;
beating the male flowers on the female parent plant completely, pinching the joints of the petals and the sepals by fingers, and tearing off the male flowers, so that all the male flowers are removed; when pollinating, the eraser at the tail of the pencil is used for dipping pollen of the male parent and coating the pollen on the stigma of the female parent; a multi-male flower can pollinate 3-5 female flowers;
step four, seed picking, namely picking the fully mature fruits and placing for half a month when the fruits begin to be colored and mature, cutting the outer edges of the peels and picking the seeds to avoid scratching the seeds; cleaning, rinsing and removing shriveled seeds after seed picking, and drying; and after the water content of the seeds is reduced to below 7%, further selecting, packaging and warehousing.
Example 5
In the embodiment of the invention, the method for producing seeds by crossbreeding melons comprises the following steps:
selecting a sandy soil with a deep coating, good permeability and low water accumulation tendency as a muskmelon hybridization seed production field, selecting a male parent and a female parent with the purity of 95%, separately soaking the male parent and the female parent in potassium permanganate solution for 0.5 hour, then soaking in warm water at 50 ℃ for 10 minutes, accelerating germination at 27 ℃ for 18 hours, and then sowing and raising seedlings;
wherein, the germination rate can be ensured by adopting the male parent and the female parent with higher purity, and the centralized pollination is convenient;
in the first step, the male parent and the female parent are planted according to a ratio of 1:5, imidacloprid missible oil is sprayed to prevent and control aphids after planting, the planting of the male parent is half a month earlier than that of the female parent, film covering treatment is carried out after seeding and seedling raising, and after accelerating germination for 18 hours, the seeds are sowed and seedling raised after exposing the cuspids;
the purpose of planting the male parents in advance is to ensure that the flowering phases of the male parents and the female parents can meet, and the purpose of higher number of the male parents is to ensure that the number of male flowers is sufficient and the pollen is more in the later period;
step two, transplanting management, namely transplanting the germinated female parent and the germinated male parent in small arched sheds respectively, wherein the transplanting plant spacing is 0.5 m; watering and topdressing when the seedlings grow to 6-8 true leaves, opening the small arched shed in high-temperature weather, and removing the small arched shed after field planting for one month, wherein the female parent carries out two-vine pruning and the male parent carries out three-vine pruning;
in the second step, after the young bud cotyledon is completely unfolded after field planting and transplanting, timely releasing air, and spraying 45% dimethoate missible oil to control pests; the top dressing mainly comprises compound fertilizer, phosphorus fertilizer, potassium fertilizer and farmyard manure; the small arched shed is opened in high temperature to prevent seedling burning;
step three, cross pollination, wherein all male flowers on the female parent plant are removed and the melon cap is isolated in a sleeving manner before pollination, so that the female parent is ensured to have no male flowers, all male flowers on the male parent are reserved, and all male flowers and young fruits are picked off; carrying out hybrid pollination after 4-5 female flowers grow on the lateral vines;
coating the stigma of the male parent male flower on the stigma of the female parent female flower, and then sheathing an isolating melon cap;
in the third step, male parent and male parent are anthers collected at the initial flowering stage of the male parent, pollen on the anther wall is removed by using a 80-mesh sieve after drying and cracking, the sieved pollen is mixed with white flour in a ratio of 1:3, female parent selected by pollination is female flower corolla reddish until the corolla cracks, and the stigma is 2 rows or 3 rows;
topping after pollination, topping off all growing points, adding water and fertilizer supply, watering once a week, and topdressing once every half month, wherein the topdressing mainly comprises urea and monopotassium phosphate, and the proportion of the urea to the monopotassium phosphate is 2: 1; and spraying a pesticide once every 10 days, wherein the pesticide comprises 1000 times of liquid of 1.5 percent of carbendazim and 500 times of liquid of 40 percent of virobuzin wettable powder for preventing and treating virus diseases;
step four, seed picking, namely picking the fully mature fruits and placing for half a month when the fruits begin to be colored and mature, cutting the outer edges of the peels and picking the seeds to avoid scratching the seeds; cleaning, rinsing and removing shriveled seeds after seed picking, and drying; and after the water content of the seeds is reduced to below 7%, further selecting, packaging and warehousing.
The invention is compared with the prior art:
comparative example 1
Selecting four female parent plants in four different periods as samples, wherein stigmas of female flowers in the four periods are respectively used as female flower stigmas of the pollination female parent after the female flower crowns are reddish to the extent that the flower crowns crack and the flower crowns crack completely for 3 days, 5 days and 7 days;
TABLE 1
Figure BDA0003190610100000081
Number of results and rate of results
As can be seen from the table 1, in the process of producing seeds of melons, selecting female parent plants pollinated in different periods has a great influence on the seed production rate, and female parent plants reddish to the stage of crown dehiscence are preferably selected as pollination female flower stigma.
Comparative example 2
Selecting three groups of samples of the male parent and the female parent with different planting ratios as comparison, wherein each group of samples comprises three independent plants, the planting ratios of the three samples are respectively 1:1, 1:5 and 1:10, and observing the germination rate.
TABLE 2
Figure BDA0003190610100000091
Germination rate
As can be seen from Table 2, the planting ratio is 1:1, which means that the number of male flowers is insufficient; when the planting ratio is 1:5, the germination rate is higher; and when the planting ratio is 1:10, the germination ratio is slightly improved compared with 1:5, and the planting ratio is preferably 1:5 to avoid seed waste.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (7)

1. The method for producing the hybrid seeds of the melons is characterized by comprising the following steps of:
selecting a sandy soil with a deep coating, good permeability and low water accumulation tendency as a muskmelon hybridization seed production field, selecting a male parent and a female parent with the purity of 95%, separately soaking the male parent and the female parent in potassium permanganate solution for 0.5 hour, then soaking in warm water at 50 ℃ for 10 minutes, accelerating germination at 27 ℃ for 18 hours, and then sowing and raising seedlings;
step two, transplanting management, namely transplanting the germinated female parent and the germinated male parent in small arched sheds respectively, wherein the transplanting plant spacing is 0.5 m; watering and topdressing when the seedlings grow to 6-8 true leaves, opening the small arched shed in high-temperature weather, and removing the small arched shed after field planting for one month, wherein the female parent carries out two-vine pruning and the male parent carries out three-vine pruning;
step three, cross pollination, wherein all male flowers on the female parent plant are removed and the melon cap is isolated in a sleeving manner before pollination, so that the female parent is ensured to have no male flowers, all male flowers on the male parent are reserved, and all male flowers and young fruits are picked off; carrying out hybrid pollination after 4-5 female flowers grow on the lateral vines;
coating the stigma of the male parent male flower on the stigma of the female parent female flower, and then sheathing an isolating melon cap;
step four, seed picking, namely picking the fully mature fruits and placing for half a month when the fruits begin to be colored and mature, cutting the outer edges of the peels and picking the seeds to avoid scratching the seeds; cleaning, rinsing and removing shriveled seeds after seed picking, and drying; and after the water content of the seeds is reduced to below 7%, further selecting, packaging and warehousing.
2. A method for crossbreeding melon as claimed in claim 1, wherein in step one, male parent and female parent are planted according to a ratio of 1:5, imidacloprid missible oil is sprayed after field planting to prevent aphids, male parent planting is half a month earlier than female parent planting, mulching treatment is carried out after seeding and seedling raising, and seeds are sown and seedling raised after 18 hours of pregermination and sharp teeth are exposed.
3. A muskmelon hybrid seed production method according to claim 2, characterized in that in the second step, after the young bud cotyledon is completely unfolded after field planting and transplanting, the wind is released in time, and 45% dimethoate missible oil is sprayed to control pests; the top dressing mainly comprises compound fertilizer, phosphorus fertilizer, potassium fertilizer and farmyard manure.
4. A method for cross breeding melon as claimed in claim 3, wherein in the third step, when the female parent plant is in full-bloom stage, the stigma from 1 day before blossom to 1 day after blossom is selected, and the stigma which is not cracked is used as the stigma of the female parent for pollination; the fruit bearing on the female parent plant is cut off, the flower and the flower bud are left, and the flowering corolla is torn off to expose the stigma.
5. A method for crossbreeding melon as claimed in claim 3, wherein in the third step, male parent flower is anther collected from the first flowering period of male parent, pollen on the anther wall is removed by 80 mesh sieve after drying and cracking, the sieved pollen is mixed with white flour in the ratio of 1:3, female parent selected by pollination is female crown reddish until the crown cracks and the stigma is 2 or 3.
6. The method for the hybrid seed production of melons according to claim 3, wherein in the third step, male flowers on the female parent plant are removed by pinching the joints between petals and sepals with fingers and tearing off the joints, and the male flowers are completely removed; when pollinating, the eraser at the tail of the pencil is used for dipping pollen of the male parent and coating the pollen on the stigma of the female parent; a multi-male flower can pollinate 3-5 female flowers.
7. A method for crossbreeding melon as claimed in any one of claims 11-6, wherein in step three, topping treatment is carried out after pollination, all growing points are knocked off, water and fertilizer supply is increased, watering is carried out once a week, top dressing is carried out once a half month, the types of top dressing are mainly urea and potassium dihydrogen phosphate, and the ratio of the two is 2: 1; and the pesticide is sprayed every 10 days, and the pesticide comprises 1000 times of liquid of 1.5 percent of carbendazim and 500 times of liquid of 40 percent of virobuzin wettable powder for preventing and treating virus diseases.
CN202110876779.3A 2021-07-31 2021-07-31 Method for producing seeds by muskmelon hybridization Withdrawn CN113711907A (en)

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Application publication date: 20211130